AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0645070

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0645070 MtrunA17_Chr4g0032451 70.345 145 43 0 13 157 10 154 5.76e-68 203
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 48 sgRNAs with CRISPR-Local

Find 209 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCTAATTTCCCCATATCTA+TGG 0.308925 4_3:+49452431 None:intergenic
ACAAGAACATATTCTTTAGA+AGG 0.321927 4_3:-49452315 Msa0645070:three_prime_UTR
TTCACAATTACTTTCTAAAT+AGG 0.322641 4_3:+49452338 None:intergenic
AACATGAGAGGACGCGATTT+AGG 0.338073 4_3:+49452259 None:intergenic
GGAAGAGGATATGTCTCTTC+AGG 0.339040 4_3:-49452914 Msa0645070:CDS
GCGAGATAGAGGTAGCAAAA+TGG 0.340749 4_3:-49452163 Msa0645070:three_prime_UTR
GCCATAGATATGGGGAAATT+AGG 0.345577 4_3:-49452432 Msa0645070:exon
AAGACATGAAGCCATAGATA+TGG 0.384347 4_3:-49452442 Msa0645070:exon
GAACAACCGGAGCATCTTGC+AGG 0.385771 4_3:-49452793 Msa0645070:CDS
TTAGTGACCACTTTACATAA+TGG 0.388432 4_3:+49452769 None:intergenic
TGCATTAATTGCGGAACAAC+CGG 0.397610 4_3:-49452806 Msa0645070:CDS
GAAGAAACGGATCATATTCC+AGG 0.409810 4_3:-49452685 Msa0645070:CDS
GCAAAATGGGTCTGACTTGT+CGG 0.426581 4_3:-49452149 Msa0645070:three_prime_UTR
TGAATTGATTGCAGCCGAAT+TGG 0.427925 4_3:-49452935 Msa0645070:CDS
TCTTCAGGATTCATTGAGAA+AGG 0.440370 4_3:-49452899 Msa0645070:CDS
CGAGATAGAGGTAGCAAAAT+GGG 0.447215 4_3:-49452162 Msa0645070:three_prime_UTR
TTAGGGATTACCAGTTAAAA+CGG 0.449452 4_3:+49455366 None:intergenic
GGTTCTCTGAGATATTTGTT+TGG 0.454082 4_3:-49452294 Msa0645070:three_prime_UTR
TGAGATATTTGTTTGGAAAG+AGG 0.461787 4_3:-49452287 Msa0645070:three_prime_UTR
AGACATGAAGCCATAGATAT+GGG 0.462291 4_3:-49452441 Msa0645070:exon
AGACATATCCTCTTCCAATT+CGG 0.468675 4_3:+49452921 None:intergenic
TGAATTTATTGAGGAAGAAA+CGG 0.471592 4_3:-49452698 Msa0645070:CDS
AATAAATAGTGGTTAAATTG+TGG 0.476337 4_3:-49452389 Msa0645070:three_prime_UTR
CAAATATGCATGCGAGATAG+AGG 0.488140 4_3:-49452174 Msa0645070:three_prime_UTR
CAATATTGATGAATTTATTG+AGG 0.493374 4_3:-49452707 Msa0645070:CDS
TAGGGATTACCAGTTAAAAC+GGG 0.499942 4_3:+49455367 None:intergenic
CAAAATGGGTCTGACTTGTC+GGG 0.501229 4_3:-49452148 Msa0645070:three_prime_UTR
TTCAGAGGCGCAGAGCTACT+AGG 0.504061 4_3:+49455335 None:intergenic
TTCCGAGAAGTCCATGGAAA+AGG 0.505472 4_3:-49453177 Msa0645070:intron
TGCAGGTCCATTATGTAAAG+TGG 0.516700 4_3:-49452776 Msa0645070:CDS
AATGGACCTGCAAGATGCTC+CGG 0.528367 4_3:+49452787 None:intergenic
ATTTGTCAATGAATTGTACA+TGG 0.532546 4_3:-49452617 Msa0645070:CDS
GATTGCAGCCGAATTGGAAG+AGG 0.533785 4_3:-49452929 Msa0645070:CDS
CAACATTTCCGAGAAGTCCA+TGG 0.547363 4_3:-49453183 Msa0645070:CDS
GTCAATGAATTGTACATGGA+TGG 0.549535 4_3:-49452613 Msa0645070:CDS
ATGGGTCTGACTTGTCGGGT+TGG 0.574302 4_3:-49452144 Msa0645070:three_prime_UTR
AAGGTATGCTGCTTTGTATG+TGG 0.584948 4_3:-49452584 Msa0645070:CDS
TGGGTGTAGGAACAAAATTG+AGG 0.593531 4_3:-49453229 Msa0645070:intron
TGTACATGGATGGACAAGAA+AGG 0.594997 4_3:-49452603 Msa0645070:CDS
ATAGTGGTTAAATTGTGGAC+TGG 0.599291 4_3:-49452384 Msa0645070:three_prime_UTR
GTACATGAAAGCGAGAAAAG+AGG 0.599519 4_3:-49452743 Msa0645070:CDS
AGTATCAAATTAAACATGAG+AGG 0.614190 4_3:+49452247 None:intergenic
GCTACTTGTTCAATGTTCAG+AGG 0.617456 4_3:+49455320 None:intergenic
AAGTAGCAGATACAACAAGT+TGG 0.636568 4_3:-49455304 Msa0645070:five_prime_UTR
GGAGATGGATGCATTAATTG+CGG 0.639286 4_3:-49452815 Msa0645070:CDS
TTGTATGTGGAGAAGTCTAG+TGG 0.643539 4_3:-49452571 Msa0645070:CDS
GACATGAAGCCATAGATATG+GGG 0.660560 4_3:-49452440 Msa0645070:exon
TGTATGTGGAGAAGTCTAGT+GGG 0.662396 4_3:-49452570 Msa0645070:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTATTAATTAAAGTACAA+AGG + chr4_3:49452595-49452614 None:intergenic 10.0%
!! ATGATTTATATAATTTATGA+TGG - chr4_3:49452982-49453001 Msa0645070:intron 10.0%
!!! TTTATATAATTTATGATGGA+TGG - chr4_3:49452986-49453005 Msa0645070:intron 15.0%
!! CAAATCTTCTATTTAAATTA+TGG + chr4_3:49453590-49453609 None:intergenic 15.0%
!! ATTAATAAAAGAGACATTTA+TGG + chr4_3:49453884-49453903 None:intergenic 15.0%
!!! CTCATTTTTGCTTATATTTT+AGG - chr4_3:49452845-49452864 Msa0645070:CDS 20.0%
!!! TTTTTGCTTATATTTTAGGA+CGG - chr4_3:49452849-49452868 Msa0645070:CDS 20.0%
!! TGACTCTTTCAAATTATTTA+AGG + chr4_3:49453130-49453149 None:intergenic 20.0%
!! TGAAAGAAACACAAAATAAT+AGG - chr4_3:49453262-49453281 Msa0645070:intron 20.0%
!! AAATAATAGGTGTAAATCAA+TGG - chr4_3:49453275-49453294 Msa0645070:intron 20.0%
!! AATAATAGGTGTAAATCAAT+GGG - chr4_3:49453276-49453295 Msa0645070:intron 20.0%
!! TATGCTATAAGCTTAAAATT+AGG - chr4_3:49453382-49453401 Msa0645070:intron 20.0%
!! AAATGAAATTCTTGAAAATG+TGG - chr4_3:49453456-49453475 Msa0645070:intron 20.0%
!!! ATCATCTAAATGATCATTTT+GGG + chr4_3:49453518-49453537 None:intergenic 20.0%
!!! TATCATCTAAATGATCATTT+TGG + chr4_3:49453519-49453538 None:intergenic 20.0%
!!! TTTTGCGTTGTTATTTTATT+TGG - chr4_3:49453623-49453642 Msa0645070:intron 20.0%
!!! TTTGCGTTGTTATTTTATTT+GGG - chr4_3:49453624-49453643 Msa0645070:intron 20.0%
!!! GTCTCTTTTATTAATCATTT+CGG - chr4_3:49453889-49453908 Msa0645070:intron 20.0%
!! TCAAATCACTATTTAGATTT+AGG - chr4_3:49453915-49453934 Msa0645070:intron 20.0%
!!! ACTATTTAGATTTAGGATAA+TGG - chr4_3:49453922-49453941 Msa0645070:intron 20.0%
!!! CTATTTAGATTTAGGATAAT+GGG - chr4_3:49453923-49453942 Msa0645070:intron 20.0%
!! AAATATTTATTATTGGGTGT+AGG - chr4_3:49454253-49454272 Msa0645070:intron 20.0%
!!! AAACATTTTTTTTCAAAAGC+AGG + chr4_3:49454392-49454411 None:intergenic 20.0%
!! CAATATTGATGAATTTATTG+AGG - chr4_3:49454788-49454807 Msa0645070:intron 20.0%
!! AAAACTGTAAGAAGTTAAAA+GGG + chr4_3:49454993-49455012 None:intergenic 20.0%
!! AAAAACTGTAAGAAGTTAAA+AGG + chr4_3:49454994-49455013 None:intergenic 20.0%
!!! GTGTTTTTTCAAATAAATAG+TGG - chr4_3:49455095-49455114 Msa0645070:intron 20.0%
!! AATAAATAGTGGTTAAATTG+TGG - chr4_3:49455106-49455125 Msa0645070:intron 20.0%
!! TTCACAATTACTTTCTAAAT+AGG + chr4_3:49455160-49455179 None:intergenic 20.0%
!!! GCTGTTTTAATAAGCTATAT+TGG + chr4_3:49452496-49452515 None:intergenic 25.0%
! TAAGCAAAAATGAGTCATAT+GGG + chr4_3:49452839-49452858 None:intergenic 25.0%
! ATAAGCAAAAATGAGTCATA+TGG + chr4_3:49452840-49452859 None:intergenic 25.0%
! AGTAACATTCAATATGTGAT+TGG + chr4_3:49452898-49452917 None:intergenic 25.0%
! TTATCATCAACTTATAGTCA+GGG + chr4_3:49453424-49453443 None:intergenic 25.0%
! ATTATCATCAACTTATAGTC+AGG + chr4_3:49453425-49453444 None:intergenic 25.0%
! GGTTAATACTGAAAATATAG+AGG - chr4_3:49453477-49453496 Msa0645070:intron 25.0%
!!! TTGCGTTGTTATTTTATTTG+GGG - chr4_3:49453625-49453644 Msa0645070:intron 25.0%
! GGTGTAAATTTAAAAGTTGA+GGG - chr4_3:49453944-49453963 Msa0645070:intron 25.0%
! AATTAATCATTAGCAATCAG+TGG + chr4_3:49454006-49454025 None:intergenic 25.0%
! TTTATGTTAAAATAGATCCC+AGG + chr4_3:49454072-49454091 None:intergenic 25.0%
! AAAAAAAATGTTTCTTGCTG+TGG - chr4_3:49454399-49454418 Msa0645070:intron 25.0%
!! AAAAAAATGTTTCTTGCTGT+GGG - chr4_3:49454400-49454419 Msa0645070:intron 25.0%
! TGAATTTATTGAGGAAGAAA+CGG - chr4_3:49454797-49454816 Msa0645070:intron 25.0%
! ATTTGTCAATGAATTGTACA+TGG - chr4_3:49454878-49454897 Msa0645070:intron 25.0%
! ACAAGAACATATTCTTTAGA+AGG - chr4_3:49455180-49455199 Msa0645070:intron 25.0%
! AGTATCAAATTAAACATGAG+AGG + chr4_3:49455251-49455270 None:intergenic 25.0%
TTAGGGATTACCAGTTAAAA+CGG + chr4_3:49452132-49452151 None:intergenic 30.0%
CATAGATCAGGAAAGTTAAA+CGG - chr4_3:49452386-49452405 Msa0645070:three_prime_UTR 30.0%
GTTAAATGCATAGGTTCTAT+GGG + chr4_3:49452534-49452553 None:intergenic 30.0%
TGTTAAATGCATAGGTTCTA+TGG + chr4_3:49452535-49452554 None:intergenic 30.0%
AAGCAAAAATGAGTCATATG+GGG + chr4_3:49452838-49452857 None:intergenic 30.0%
ATTTAAGCAAGCAAAAAGCT+AGG - chr4_3:49453057-49453076 Msa0645070:intron 30.0%
!! TGTTGATGATATTGAAGATG+TGG - chr4_3:49453561-49453580 Msa0645070:intron 30.0%
!! GGCATATTTTTACTTAGAGT+CGG - chr4_3:49453646-49453665 Msa0645070:intron 30.0%
!! GCATATTTTTACTTAGAGTC+GGG - chr4_3:49453647-49453666 Msa0645070:intron 30.0%
GGGTGTAAATTTAAAAGTTG+AGG - chr4_3:49453943-49453962 Msa0645070:intron 30.0%
! CTTGTGGATGATTATTGATT+AGG - chr4_3:49454131-49454150 Msa0645070:intron 30.0%
! GGATGATTATTGATTAGGAT+CGG - chr4_3:49454136-49454155 Msa0645070:intron 30.0%
CTGATTTATGCACAATCTAT+TGG + chr4_3:49454225-49454244 None:intergenic 30.0%
AACTTGAATTTGGCTGTAAA+TGG - chr4_3:49454476-49454495 Msa0645070:intron 30.0%
!! TTACGCTACTATTTTGAAAG+AGG - chr4_3:49454659-49454678 Msa0645070:intron 30.0%
!! TACTATTTTGAAAGAGGAGA+TGG - chr4_3:49454665-49454684 Msa0645070:intron 30.0%
TTAGTGACCACTTTACATAA+TGG + chr4_3:49454729-49454748 None:intergenic 30.0%
! ACAGTTTTTCTTGTCACTAT+AGG - chr4_3:49455005-49455024 Msa0645070:intron 30.0%
TGAGATATTTGTTTGGAAAG+AGG - chr4_3:49455208-49455227 Msa0645070:intron 30.0%
TAGGGATTACCAGTTAAAAC+GGG + chr4_3:49452131-49452150 None:intergenic 35.0%
!!! GCTACTAGGTTTTGTTGTTA+GGG + chr4_3:49452149-49452168 None:intergenic 35.0%
!!! AGCTACTAGGTTTTGTTGTT+AGG + chr4_3:49452150-49452169 None:intergenic 35.0%
AAGTAGCAGATACAACAAGT+TGG - chr4_3:49452191-49452210 Msa0645070:three_prime_UTR 35.0%
AGAACCTATGCATTTAACAG+TGG - chr4_3:49452536-49452555 Msa0645070:intron 35.0%
AGCAAAAATGAGTCATATGG+GGG + chr4_3:49452837-49452856 None:intergenic 35.0%
!!! TTGCTTATATTTTAGGACGG+AGG - chr4_3:49452852-49452871 Msa0645070:CDS 35.0%
!!! TGCTTATATTTTAGGACGGA+GGG - chr4_3:49452853-49452872 Msa0645070:CDS 35.0%
!! TGGATGGAGTACTAAGTTTT+AGG - chr4_3:49453002-49453021 Msa0645070:intron 35.0%
TGTACATTACGCCATGTTAT+TGG - chr4_3:49453157-49453176 Msa0645070:intron 35.0%
AACATTGCTGTCCAATAACA+TGG + chr4_3:49453171-49453190 None:intergenic 35.0%
GTTCAATGTGTGCAACTTAA+CGG - chr4_3:49453188-49453207 Msa0645070:CDS 35.0%
! TGGTGCAAGCCTTATATTTA+AGG - chr4_3:49453229-49453248 Msa0645070:intron 35.0%
! GGTGCAAGCCTTATATTTAA+GGG - chr4_3:49453230-49453249 Msa0645070:intron 35.0%
! GTGCAAGCCTTATATTTAAG+GGG - chr4_3:49453231-49453250 Msa0645070:intron 35.0%
! TGCAAGCCTTATATTTAAGG+GGG - chr4_3:49453232-49453251 Msa0645070:intron 35.0%
CTTGAACCCCCTTAAATATA+AGG + chr4_3:49453241-49453260 None:intergenic 35.0%
ACATAAGCTGTCAATAGCAT+AGG + chr4_3:49453351-49453370 None:intergenic 35.0%
GCTATTGACAGCTTATGTTT+AGG - chr4_3:49453353-49453372 Msa0645070:intron 35.0%
TCCATAAATCAGTATGACAG+CGG + chr4_3:49453829-49453848 None:intergenic 35.0%
AGTGACTACTTCAATACAGA+GGG + chr4_3:49454029-49454048 None:intergenic 35.0%
AAGTGACTACTTCAATACAG+AGG + chr4_3:49454030-49454049 None:intergenic 35.0%
TCCACAGACTAATCAAACTT+AGG + chr4_3:49454107-49454126 None:intergenic 35.0%
! TGATATCCAATAGTGTTGCT+AGG - chr4_3:49454184-49454203 Msa0645070:intron 35.0%
AGAAGAGAAGAATAAGCAAC+CGG - chr4_3:49454451-49454470 Msa0645070:intron 35.0%
! ACAGCCAAATTCAAGTTTAC+CGG + chr4_3:49454473-49454492 None:intergenic 35.0%
TGAATTTGGCTGTAAATGGA+TGG - chr4_3:49454480-49454499 Msa0645070:intron 35.0%
GAATTTGGCTGTAAATGGAT+GGG - chr4_3:49454481-49454500 Msa0645070:intron 35.0%
TGCTTACTAGAAACTGTTTG+TGG - chr4_3:49454523-49454542 Msa0645070:intron 35.0%
AGACATATCCTCTTCCAATT+CGG + chr4_3:49454577-49454596 None:intergenic 35.0%
TCTTCAGGATTCATTGAGAA+AGG - chr4_3:49454596-49454615 Msa0645070:intron 35.0%
AAAAACCCTAAAAGAGAACG+AGG - chr4_3:49454635-49454654 Msa0645070:intron 35.0%
! GTAAACCTCGTTCTCTTTTA+GGG + chr4_3:49454643-49454662 None:intergenic 35.0%
! ATCTCTATTTTCAATGCACC+TGG + chr4_3:49454831-49454850 None:intergenic 35.0%
GTCAATGAATTGTACATGGA+TGG - chr4_3:49454882-49454901 Msa0645070:intron 35.0%
AAGACATGAAGCCATAGATA+TGG - chr4_3:49455053-49455072 Msa0645070:intron 35.0%
AGACATGAAGCCATAGATAT+GGG - chr4_3:49455054-49455073 Msa0645070:intron 35.0%
ATAGTGGTTAAATTGTGGAC+TGG - chr4_3:49455111-49455130 Msa0645070:intron 35.0%
!! GGTTCTCTGAGATATTTGTT+TGG - chr4_3:49455201-49455220 Msa0645070:intron 35.0%
TCAAAAAAAGAGGGCTAAAC+GGG + chr4_3:49455377-49455396 None:intergenic 35.0%
GTCAAAAAAAGAGGGCTAAA+CGG + chr4_3:49455378-49455397 None:intergenic 35.0%
GCTACTTGTTCAATGTTCAG+AGG + chr4_3:49452178-49452197 None:intergenic 40.0%
CATCATCATCATCATCATCC+AGG - chr4_3:49452234-49452253 Msa0645070:three_prime_UTR 40.0%
CGAACAGAAAAAGAGAGAAG+GGG + chr4_3:49452267-49452286 None:intergenic 40.0%
CCGAACAGAAAAAGAGAGAA+GGG + chr4_3:49452268-49452287 None:intergenic 40.0%
! CCCTTCTCTCTTTTTCTGTT+CGG - chr4_3:49452265-49452284 Msa0645070:three_prime_UTR 40.0%
TCCGAACAGAAAAAGAGAGA+AGG + chr4_3:49452269-49452288 None:intergenic 40.0%
AACGCCACTGTTAAATGCAT+AGG + chr4_3:49452543-49452562 None:intergenic 40.0%
AAGCAAGCAAAAAGCTAGGT+AGG - chr4_3:49453061-49453080 Msa0645070:intron 40.0%
!! AGGTGTAAATCAATGGGTTG+AGG - chr4_3:49453282-49453301 Msa0645070:intron 40.0%
!! GGTGTAAATCAATGGGTTGA+GGG - chr4_3:49453283-49453302 Msa0645070:intron 40.0%
!! GTGTAAATCAATGGGTTGAG+GGG - chr4_3:49453284-49453303 Msa0645070:intron 40.0%
!! TGTAAATCAATGGGTTGAGG+GGG - chr4_3:49453285-49453304 Msa0645070:intron 40.0%
ACTTATAGTCAGGGTGTGTA+TGG + chr4_3:49453415-49453434 None:intergenic 40.0%
!! TCCGCTGTCATACTGATTTA+TGG - chr4_3:49453825-49453844 Msa0645070:intron 40.0%
CAGAGCTAGCTAATAACTAG+GGG + chr4_3:49453983-49454002 None:intergenic 40.0%
GCAGAGCTAGCTAATAACTA+GGG + chr4_3:49453984-49454003 None:intergenic 40.0%
TTTATACAAGCCTATGCTCC+TGG - chr4_3:49454051-49454070 Msa0645070:intron 40.0%
TTATACAAGCCTATGCTCCT+GGG - chr4_3:49454052-49454071 Msa0645070:intron 40.0%
! AAAATAGATCCCAGGAGCAT+AGG + chr4_3:49454064-49454083 None:intergenic 40.0%
!! GCCTAAGTTTGATTAGTCTG+TGG - chr4_3:49454103-49454122 Msa0645070:intron 40.0%
TTAGTCTGTGGATGTTCTTG+TGG - chr4_3:49454115-49454134 Msa0645070:intron 40.0%
CAAAAGCCTAGCAACACTAT+TGG + chr4_3:49454193-49454212 None:intergenic 40.0%
!! CAATAGTGTTGCTAGGCTTT+TGG - chr4_3:49454191-49454210 Msa0645070:intron 40.0%
TGGGTGTAGGAACAAAATTG+AGG - chr4_3:49454266-49454285 Msa0645070:intron 40.0%
GCAACCGGTAAACTTGAATT+TGG - chr4_3:49454466-49454485 Msa0645070:intron 40.0%
TGAATTGATTGCAGCCGAAT+TGG - chr4_3:49454560-49454579 Msa0645070:intron 40.0%
! CGTAAACCTCGTTCTCTTTT+AGG + chr4_3:49454644-49454663 None:intergenic 40.0%
GGAGATGGATGCATTAATTG+CGG - chr4_3:49454680-49454699 Msa0645070:intron 40.0%
TGCATTAATTGCGGAACAAC+CGG - chr4_3:49454689-49454708 Msa0645070:intron 40.0%
TGCAGGTCCATTATGTAAAG+TGG - chr4_3:49454719-49454738 Msa0645070:intron 40.0%
GTACATGAAAGCGAGAAAAG+AGG - chr4_3:49454752-49454771 Msa0645070:intron 40.0%
GAAGAAACGGATCATATTCC+AGG - chr4_3:49454810-49454829 Msa0645070:intron 40.0%
TGTACATGGATGGACAAGAA+AGG - chr4_3:49454892-49454911 Msa0645070:intron 40.0%
!! AAGGTATGCTGCTTTGTATG+TGG - chr4_3:49454911-49454930 Msa0645070:intron 40.0%
TTGTATGTGGAGAAGTCTAG+TGG - chr4_3:49454924-49454943 Msa0645070:intron 40.0%
TGTATGTGGAGAAGTCTAGT+GGG - chr4_3:49454925-49454944 Msa0645070:intron 40.0%
! CTAGTGGGTAAGTTATACAC+TGG - chr4_3:49454940-49454959 Msa0645070:intron 40.0%
GACATGAAGCCATAGATATG+GGG - chr4_3:49455055-49455074 Msa0645070:intron 40.0%
GCCATAGATATGGGGAAATT+AGG - chr4_3:49455063-49455082 Msa0645070:intron 40.0%
GCCTAATTTCCCCATATCTA+TGG + chr4_3:49455067-49455086 None:intergenic 40.0%
CAAATATGCATGCGAGATAG+AGG - chr4_3:49455321-49455340 Msa0645070:five_prime_UTR 40.0%
CGAGATAGAGGTAGCAAAAT+GGG - chr4_3:49455333-49455352 Msa0645070:five_prime_UTR 40.0%
TTCCTTCCCCTTTCTCGATA+CGG - chr4_3:49452291-49452310 Msa0645070:three_prime_UTR 45.0%
TATGTATGCGATTCCATCGC+TGG - chr4_3:49452325-49452344 Msa0645070:three_prime_UTR 45.0%
CGCGATTCACAACATAGATC+AGG - chr4_3:49452374-49452393 Msa0645070:three_prime_UTR 45.0%
AGCTGTCAATAGCATAGGCA+GGG + chr4_3:49453346-49453365 None:intergenic 45.0%
AAGCTGTCAATAGCATAGGC+AGG + chr4_3:49453347-49453366 None:intergenic 45.0%
GACAGCGGAGTATACAATCA+GGG + chr4_3:49453814-49453833 None:intergenic 45.0%
TGACAGCGGAGTATACAATC+AGG + chr4_3:49453815-49453834 None:intergenic 45.0%
GGCAGAGCTAGCTAATAACT+AGG + chr4_3:49453985-49454004 None:intergenic 45.0%
CAACATTTCCGAGAAGTCCA+TGG - chr4_3:49454312-49454331 Msa0645070:intron 45.0%
TTCCGAGAAGTCCATGGAAA+AGG - chr4_3:49454318-49454337 Msa0645070:intron 45.0%
! CACCTTTTCCATGGACTTCT+CGG + chr4_3:49454323-49454342 None:intergenic 45.0%
! TGTATGTCGCACCTTTTCCA+TGG + chr4_3:49454332-49454351 None:intergenic 45.0%
GGAAGAGGATATGTCTCTTC+AGG - chr4_3:49454581-49454600 Msa0645070:intron 45.0%
AACATGAGAGGACGCGATTT+AGG + chr4_3:49455239-49455258 None:intergenic 45.0%
GCGAGATAGAGGTAGCAAAA+TGG - chr4_3:49455332-49455351 Msa0645070:five_prime_UTR 45.0%
! GCAAAATGGGTCTGACTTGT+CGG - chr4_3:49455346-49455365 Msa0645070:five_prime_UTR 45.0%
! CAAAATGGGTCTGACTTGTC+GGG - chr4_3:49455347-49455366 Msa0645070:five_prime_UTR 45.0%
!! TTATTCTAAATATTTATTAT+TGG - chr4_3:49454246-49454265 Msa0645070:intron 5.0%
!! TATTCTAAATATTTATTATT+GGG - chr4_3:49454247-49454266 Msa0645070:intron 5.0%
GAGAGAAGGGGAAACAAACC+TGG + chr4_3:49452255-49452274 None:intergenic 50.0%
CGGTTGAGTAGACGACTGTT+TGG - chr4_3:49452406-49452425 Msa0645070:three_prime_UTR 50.0%
CATTTAACAGTGGCGTTGCC+AGG - chr4_3:49452546-49452565 Msa0645070:intron 50.0%
GGTTACAGATGAGAGAGAGC+TGG - chr4_3:49453209-49453228 Msa0645070:exon 50.0%
GCTGTCAATAGCATAGGCAG+GGG + chr4_3:49453345-49453364 None:intergenic 50.0%
TAGAGTCGACCCTAGCAAAC+TGG + chr4_3:49453676-49453695 None:intergenic 50.0%
! CTTGCTGTGGGCAAAACTGA+AGG - chr4_3:49454412-49454431 Msa0645070:intron 50.0%
GATTGCAGCCGAATTGGAAG+AGG - chr4_3:49454566-49454585 Msa0645070:intron 50.0%
AATGGACCTGCAAGATGCTC+CGG + chr4_3:49454711-49454730 None:intergenic 50.0%
! TTCAGAGGCGCAGAGCTACT+AGG + chr4_3:49452163-49452182 None:intergenic 55.0%
CTTCCCCTTTCTCGATACGG+CGG - chr4_3:49452294-49452313 Msa0645070:three_prime_UTR 55.0%
TGAATCGCGCCGCATTGAAG+GGG + chr4_3:49452363-49452382 None:intergenic 55.0%
GTGAATCGCGCCGCATTGAA+GGG + chr4_3:49452364-49452383 None:intergenic 55.0%
TGTGAATCGCGCCGCATTGA+AGG + chr4_3:49452365-49452384 None:intergenic 55.0%
TGCATCCCCTGGATACAAGC+TGG - chr4_3:49453314-49453333 Msa0645070:intron 55.0%
GTCAATAGCATAGGCAGGGG+CGG + chr4_3:49453342-49453361 None:intergenic 55.0%
! AGTCGGGTCTCCAGTTTGCT+AGG - chr4_3:49453663-49453682 Msa0645070:intron 55.0%
!! GTCGGGTCTCCAGTTTGCTA+GGG - chr4_3:49453664-49453683 Msa0645070:intron 55.0%
! GAACAACCGGAGCATCTTGC+AGG - chr4_3:49454702-49454721 Msa0645070:intron 55.0%
! ATGGGTCTGACTTGTCGGGT+TGG - chr4_3:49455351-49455370 Msa0645070:five_prime_UTR 55.0%
CGCCGTATCGAGAAAGGGGA+AGG + chr4_3:49452296-49452315 None:intergenic 60.0%
TGCGCCGCCGTATCGAGAAA+GGG + chr4_3:49452301-49452320 None:intergenic 60.0%
CCGCATTGAAGGGGCGGATA+CGG + chr4_3:49452354-49452373 None:intergenic 60.0%
CCGTATCCGCCCCTTCAATG+CGG - chr4_3:49452351-49452370 Msa0645070:three_prime_UTR 60.0%
CGGAGCCAGCTTGTATCCAG+GGG + chr4_3:49453322-49453341 None:intergenic 60.0%
GCGGAGCCAGCTTGTATCCA+GGG + chr4_3:49453323-49453342 None:intergenic 60.0%
GCGCCGCCGTATCGAGAAAG+GGG + chr4_3:49452300-49452319 None:intergenic 65.0%
GTGCGCCGCCGTATCGAGAA+AGG + chr4_3:49452302-49452321 None:intergenic 65.0%
ATCGCGCCGCATTGAAGGGG+CGG + chr4_3:49452360-49452379 None:intergenic 65.0%
AGTGGCGTTGCCAGGACCCT+GGG - chr4_3:49452554-49452573 Msa0645070:intron 65.0%
GTTGCCAGGACCCTGGGTCA+GGG - chr4_3:49452560-49452579 Msa0645070:intron 65.0%
TTGCCAGGACCCTGGGTCAG+GGG - chr4_3:49452561-49452580 Msa0645070:intron 65.0%
GTGTGAACCCCCCTGACCCA+GGG + chr4_3:49452573-49452592 None:intergenic 65.0%
GGCGGAGCCAGCTTGTATCC+AGG + chr4_3:49453324-49453343 None:intergenic 65.0%
GCGGATACGGCAGCCAGCGA+TGG + chr4_3:49452341-49452360 None:intergenic 70.0%
CAGTGGCGTTGCCAGGACCC+TGG - chr4_3:49452553-49452572 Msa0645070:intron 70.0%
CGTTGCCAGGACCCTGGGTC+AGG - chr4_3:49452559-49452578 Msa0645070:intron 70.0%
TGCCAGGACCCTGGGTCAGG+GGG - chr4_3:49452562-49452581 Msa0645070:intron 70.0%
GGTGTGAACCCCCCTGACCC+AGG + chr4_3:49452574-49452593 None:intergenic 70.0%
GGGGGTTCAGCTGCATCCCC+TGG - chr4_3:49453303-49453322 Msa0645070:intron 70.0%
GCCAGGACCCTGGGTCAGGG+GGG - chr4_3:49452563-49452582 Msa0645070:intron 75.0%
ACCCCCCTGACCCAGGGTCC+TGG + chr4_3:49452567-49452586 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 49452120 49455397 49452120 ID=Msa0645070;Name=Msa0645070
chr4_3 mRNA 49452120 49455397 49452120 ID=Msa0645070-mRNA-1;Parent=Msa0645070;Name=Msa0645070-mRNA-1;_AED=0.29;_eAED=0.29;_QI=165|1|1|1|0.66|0.75|4|328|157
chr4_3 exon 49452120 49452490 49452120 ID=Msa0645070-mRNA-1:exon:11835;Parent=Msa0645070-mRNA-1
chr4_3 exon 49452571 49452965 49452571 ID=Msa0645070-mRNA-1:exon:11834;Parent=Msa0645070-mRNA-1
chr4_3 exon 49453178 49453242 49453178 ID=Msa0645070-mRNA-1:exon:11833;Parent=Msa0645070-mRNA-1
chr4_3 exon 49455262 49455397 49455262 ID=Msa0645070-mRNA-1:exon:11832;Parent=Msa0645070-mRNA-1
chr4_3 five_prime_UTR 49455262 49455397 49455262 ID=Msa0645070-mRNA-1:five_prime_utr;Parent=Msa0645070-mRNA-1
chr4_3 five_prime_UTR 49453214 49453242 49453214 ID=Msa0645070-mRNA-1:five_prime_utr;Parent=Msa0645070-mRNA-1
chr4_3 CDS 49453178 49453213 49453178 ID=Msa0645070-mRNA-1:cds;Parent=Msa0645070-mRNA-1
chr4_3 CDS 49452571 49452965 49452571 ID=Msa0645070-mRNA-1:cds;Parent=Msa0645070-mRNA-1
chr4_3 CDS 49452448 49452490 49452448 ID=Msa0645070-mRNA-1:cds;Parent=Msa0645070-mRNA-1
chr4_3 three_prime_UTR 49452120 49452447 49452120 ID=Msa0645070-mRNA-1:three_prime_utr;Parent=Msa0645070-mRNA-1
Gene Sequence

>Msa0645070

ATGAAGTTCAACATTTCCGAGAAGTCCATGGAAAAGCAAATTGATGAATTGATTGCAGCCGAATTGGAAGAGGATATGTCTCTTCAGGATTCATTGAGAAAGGTTAATGACTTGTTTATAAAAACCCTAAAAGAGAACGAGGTTTACGCTACTATTTTGAAAGAGGAGATGGATGCATTAATTGCGGAACAACCGGAGCATCTTGCAGGTCCATTATGTAAAGTGGTCACTAAAATGTACATGAAAGCGAGAAAAGAGGTCAGTGAATTGTACAATATTGATGAATTTATTGAGGAAGAAACGGATCATATTCCAGGTGCATTGAAAATAGAGATGATTAAATCGTACATGAAATTGCAAAAATTTGTCAATGAATTGTACATGGATGGACAAGAAAGGTATGCTGCTTTGTATGTGGAGAAGTCTAGTGGGCTAATCAAAGACAAAACAGAAAAGCAAGACATGAAGCCATAG
Protein sequence

>Msa0645070

MKFNISEKSMEKQIDELIAAELEEDMSLQDSLRKVNDLFIKTLKENEVYATILKEEMDALIAEQPEHLAGPLCKVVTKMYMKARKEVSELYNIDEFIEEETDHIPGALKIEMIKSYMKLQKFVNELYMDGQERYAALYVEKSSGLIKDKTEKQDMKP*