AlfalfaGEDB | Msa0645070

M. sativa cultivar zhongmu-4 / Msa0645070

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0645070	XP_024637040.1	68.212	151	48	0	7	157	16	166	3.28e-64	204

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0645070	A0A072UKD9	70.345	145	43	0	13	157	10	154	2.99e-64	203

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0603670	Msa0645070	0.800048	1.730067e-48	-8.615850e-47
Msa0645070	Msa0764690	0.808164	3.543319e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0645070	MtrunA17_Chr4g0032451	70.345	145	43	0	13	157	10	154	5.76e-68	203

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 48 sgRNAs with CRISPR-Local

Find 209 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACATGAGAGGACGCGATTT+AGG	0.338073	4_3:+49452259	None:intergenic
AAGACATGAAGCCATAGATA+TGG	0.384347	4_3:-49452442	Msa0645070:exon
AAGGTATGCTGCTTTGTATG+TGG	0.584948	4_3:-49452584	Msa0645070:CDS
AAGTAGCAGATACAACAAGT+TGG	0.636568	4_3:-49455304	Msa0645070:five_prime_UTR
AATAAATAGTGGTTAAATTG+TGG	0.476337	4_3:-49452389	Msa0645070:three_prime_UTR
AATGGACCTGCAAGATGCTC+CGG	0.528367	4_3:+49452787	None:intergenic
ACAAGAACATATTCTTTAGA+AGG	0.321927	4_3:-49452315	Msa0645070:three_prime_UTR
AGACATATCCTCTTCCAATT+CGG	0.468675	4_3:+49452921	None:intergenic
AGACATGAAGCCATAGATAT+GGG	0.462291	4_3:-49452441	Msa0645070:exon
AGTATCAAATTAAACATGAG+AGG	0.614190	4_3:+49452247	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTAGGGATTACCAGTTAAAA+CGG	+	chr4_3:49452132-49452151	None:intergenic	30.0%
CATAGATCAGGAAAGTTAAA+CGG	-	chr4_3:49452386-49452405	Msa0645070:three_prime_UTR	30.0%
GTTAAATGCATAGGTTCTAT+GGG	+	chr4_3:49452534-49452553	None:intergenic	30.0%
TGTTAAATGCATAGGTTCTA+TGG	+	chr4_3:49452535-49452554	None:intergenic	30.0%
AAGCAAAAATGAGTCATATG+GGG	+	chr4_3:49452838-49452857	None:intergenic	30.0%
ATTTAAGCAAGCAAAAAGCT+AGG	-	chr4_3:49453057-49453076	Msa0645070:intron	30.0%
GGGTGTAAATTTAAAAGTTG+AGG	-	chr4_3:49453943-49453962	Msa0645070:intron	30.0%
CTGATTTATGCACAATCTAT+TGG	+	chr4_3:49454225-49454244	None:intergenic	30.0%
AACTTGAATTTGGCTGTAAA+TGG	-	chr4_3:49454476-49454495	Msa0645070:intron	30.0%
TTAGTGACCACTTTACATAA+TGG	+	chr4_3:49454729-49454748	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_3	gene	49452120	49455397	49452120	ID=Msa0645070;Name=Msa0645070
chr4_3	mRNA	49452120	49455397	49452120	ID=Msa0645070-mRNA-1;Parent=Msa0645070;Name=Msa0645070-mRNA-1;_AED=0.29;_eAED=0.29;_QI=165\|1\|1\|1\|0.66\|0.75\|4\|328\|157
chr4_3	exon	49452120	49452490	49452120	ID=Msa0645070-mRNA-1:exon:11835;Parent=Msa0645070-mRNA-1
chr4_3	exon	49452571	49452965	49452571	ID=Msa0645070-mRNA-1:exon:11834;Parent=Msa0645070-mRNA-1
chr4_3	exon	49453178	49453242	49453178	ID=Msa0645070-mRNA-1:exon:11833;Parent=Msa0645070-mRNA-1
chr4_3	exon	49455262	49455397	49455262	ID=Msa0645070-mRNA-1:exon:11832;Parent=Msa0645070-mRNA-1
chr4_3	five_prime_UTR	49455262	49455397	49455262	ID=Msa0645070-mRNA-1:five_prime_utr;Parent=Msa0645070-mRNA-1
chr4_3	five_prime_UTR	49453214	49453242	49453214	ID=Msa0645070-mRNA-1:five_prime_utr;Parent=Msa0645070-mRNA-1
chr4_3	CDS	49453178	49453213	49453178	ID=Msa0645070-mRNA-1:cds;Parent=Msa0645070-mRNA-1
chr4_3	CDS	49452571	49452965	49452571	ID=Msa0645070-mRNA-1:cds;Parent=Msa0645070-mRNA-1
chr4_3	CDS	49452448	49452490	49452448	ID=Msa0645070-mRNA-1:cds;Parent=Msa0645070-mRNA-1
chr4_3	three_prime_UTR	49452120	49452447	49452120	ID=Msa0645070-mRNA-1:three_prime_utr;Parent=Msa0645070-mRNA-1

Gene Sequence

>Msa0645070

ATGAAGTTCAACATTTCCGAGAAGTCCATGGAAAAGCAAATTGATGAATTGATTGCAGCCGAATTGGAAGAGGATATGTCTCTTCAGGATTCATTGAGAAAGGTTAATGACTTGTTTATAAAAACCCTAAAAGAGAACGAGGTTTACGCTACTATTTTGAAAGAGGAGATGGATGCATTAATTGCGGAACAACCGGAGCATCTTGCAGGTCCATTATGTAAAGTGGTCACTAAAATGTACATGAAAGCGAGAAAAGAGGTCAGTGAATTGTACAATATTGATGAATTTATTGAGGAAGAAACGGATCATATTCCAGGTGCATTGAAAATAGAGATGATTAAATCGTACATGAAATTGCAAAAATTTGTCAATGAATTGTACATGGATGGACAAGAAAGGTATGCTGCTTTGTATGTGGAGAAGTCTAGTGGGCTAATCAAAGACAAAACAGAAAAGCAAGACATGAAGCCATAG

Protein sequence

>Msa0645070

MKFNISEKSMEKQIDELIAAELEEDMSLQDSLRKVNDLFIKTLKENEVYATILKEEMDALIAEQPEHLAGPLCKVVTKMYMKARKEVSELYNIDEFIEEETDHIPGALKIEMIKSYMKLQKFVNELYMDGQERYAALYVEKSSGLIKDKTEKQDMKP*