AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0645080

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0645080 MtrunA17_Chr4g0032451 78.472 144 31 0 10 153 10 153 3.96e-77 226
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 59 sgRNAs with CRISPR-Local

Find 163 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTCAGAGGCGCCGAGCTAC+TGG 0.271882 4_3:+49461086 None:intergenic
AGACATATCCTCTTCCAATT+TGG 0.292212 4_3:+49459464 None:intergenic
CATGAGAGAGGACGTGATTT+AGG 0.304402 4_3:+49458792 None:intergenic
CGCGAACCTCGTTCTCAAAT+AGG 0.305472 4_3:+49459397 None:intergenic
AAAATGAGCTGAATCAATTT+AGG 0.306772 4_3:+49458609 None:intergenic
ACAAGAACATATTCTTTAGA+AGG 0.321927 4_3:-49458847 Msa0645080:three_prime_UTR
CAGAGATAGAGGTAGCAAAA+TGG 0.323011 4_3:-49458704 Msa0645080:three_prime_UTR
TTACTGACCTCTTTACATAA+TGG 0.325529 4_3:+49459312 None:intergenic
GGAAGAGGATATGTCTCTTC+AGG 0.339040 4_3:-49459457 Msa0645080:CDS
ACCTCTATTGTCAATGCAAC+TGG 0.362982 4_3:+49459210 None:intergenic
TGAATTGATTGCAGCCAAAT+TGG 0.366887 4_3:-49459478 Msa0645080:CDS
CTCAGACATTGTTTAGAAAG+AGG 0.416468 4_3:-49458820 Msa0645080:three_prime_UTR
GCGAACCTCGTTCTCAAATA+GGG 0.423289 4_3:+49459398 None:intergenic
TCTTCAGGATTCATTGAGAA+AGG 0.435632 4_3:-49459442 Msa0645080:CDS
TGATATAATTGAGGAACAAT+CGG 0.438496 4_3:-49459241 Msa0645080:CDS
TTCAGAGGCGCCGAGCTACT+GGG 0.438574 4_3:+49461087 None:intergenic
TGTATGTCGCACCTCATTCT+CGG 0.460727 4_3:+49459648 None:intergenic
AAACAACCGGAGCATATTGC+AGG 0.464852 4_3:-49459336 Msa0645080:CDS
GATATAATTGAGGAACAATC+GGG 0.473104 4_3:-49459240 Msa0645080:CDS
TGCAATAATTGAGAAACAAC+CGG 0.476453 4_3:-49459349 Msa0645080:CDS
AGAGATAGAGGTAGCAAAAT+GGG 0.478511 4_3:-49458703 Msa0645080:three_prime_UTR
CAATCCACTGAAGATATGTA+AGG 0.478649 4_3:-49458934 Msa0645080:three_prime_UTR
CAATATTGATGATATAATTG+AGG 0.489275 4_3:-49459250 Msa0645080:CDS
CATCTATTAGGATAGTAAAG+TGG 0.499249 4_3:+49458547 None:intergenic
ATTTATCGATGAATTGTACA+TGG 0.501762 4_3:-49459160 Msa0645080:CDS
AAATATGCATGCAGAGATAG+AGG 0.505997 4_3:-49458715 Msa0645080:three_prime_UTR
GTTCAACATTTCCGAGAATG+AGG 0.508451 4_3:-49459659 Msa0645080:intron
CATAGATTGCGGTCAAATTG+TGG 0.513642 4_3:-49458973 Msa0645080:exon
ATCGATGAATTGTACATGGA+TGG 0.519577 4_3:-49459156 Msa0645080:CDS
GATTGCAGCCAAATTGGAAG+AGG 0.525218 4_3:-49459472 Msa0645080:CDS
TGCAGGTCCATTATGTAAAG+AGG 0.525512 4_3:-49459319 Msa0645080:CDS
ATCTATTAGGATAGTAAAGT+GGG 0.548839 4_3:+49458548 None:intergenic
AACAACAAAACCCAGTAGCT+CGG 0.550766 4_3:-49461097 Msa0645080:five_prime_UTR
TCCAGTTGCATTGACAATAG+AGG 0.563787 4_3:-49459211 Msa0645080:CDS
GCAAAATGGGTCTGATTCGT+CGG 0.564076 4_3:-49458690 Msa0645080:three_prime_UTR
TTGAGTCGAAGTGTTGGACT+CGG 0.572709 4_3:-49458635 Msa0645080:three_prime_UTR
AATGGACCTGCAATATGCTC+CGG 0.574719 4_3:+49459330 None:intergenic
TGCTATGTTGAAAGAGGAGA+CGG 0.574952 4_3:-49459373 Msa0645080:CDS
TACAGATTGAGTCGAAGTGT+TGG 0.577991 4_3:-49458641 Msa0645080:three_prime_UTR
TCGCGCTGCTATGTTGAAAG+AGG 0.578051 4_3:-49459379 Msa0645080:CDS
ACTTCATACATCTCATGACC+AGG 0.583348 4_3:+49458898 None:intergenic
TGTATCTGCTACTTGTTCAG+AGG 0.584728 4_3:+49461072 None:intergenic
AAGGTATGCTGCTTTGTATG+TGG 0.584948 4_3:-49459127 Msa0645080:CDS
TGTACATGGATGGACAAGAA+AGG 0.594997 4_3:-49459146 Msa0645080:CDS
GGGCTTAAACATGTGGCTGA+CGG 0.596336 4_3:-49458571 Msa0645080:three_prime_UTR
AATCCACTGAAGATATGTAA+GGG 0.600170 4_3:-49458933 Msa0645080:three_prime_UTR
AACTGCCCTATTTGAGAACG+AGG 0.600432 4_3:-49459403 Msa0645080:CDS
TGGGTGCAGGAACAAAATTG+AGG 0.605697 4_3:-49459702 Msa0645080:intron
ATCCACTGAAGATATGTAAG+GGG 0.622846 4_3:-49458932 Msa0645080:three_prime_UTR
TATCAAATTAAACATGAGAG+AGG 0.633734 4_3:+49458780 None:intergenic
AAGTAGCAGATACAACAAGT+TGG 0.636568 4_3:-49461063 Msa0645080:five_prime_UTR
GTTAGGTTTACGATTTAACA+CGG 0.641261 4_3:+49461117 None:intergenic
AGACAGAAAGTCATAGATTG+CGG 0.660115 4_3:-49458984 Msa0645080:exon
TTAGGTTTACGATTTAACAC+GGG 0.665939 4_3:+49461118 None:intergenic
CTTCATACATCTCATGACCA+GGG 0.674119 4_3:+49458899 None:intergenic
TCCACTGAAGATATGTAAGG+GGG 0.683717 4_3:-49458931 Msa0645080:three_prime_UTR
TGTATGTGGAGAAGTCTAGT+CGG 0.687076 4_3:-49459113 Msa0645080:intron
ATACATGAAACTGAGAACAG+AGG 0.729964 4_3:-49459286 Msa0645080:CDS
ATATAATTGAGGAACAATCG+GGG 0.746906 4_3:-49459239 Msa0645080:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATAATTTTTAAACAAAAAG+AGG + chr4_3:49459339-49459358 None:intergenic 10.0%
!! CAAATCTTCTATTTAAATTA+TGG + chr4_3:49459206-49459225 None:intergenic 15.0%
!! ATTAATAAAAGAGACATTTA+TGG + chr4_3:49459558-49459577 None:intergenic 15.0%
!! TATGCTATAAGCTTAAAATT+AGG - chr4_3:49458998-49459017 Msa0645080:CDS 20.0%
!! AAATGAAATTCTTGAAAATG+TGG - chr4_3:49459072-49459091 Msa0645080:intron 20.0%
!!! ATCATCTAAATGATCATTTT+GGG + chr4_3:49459134-49459153 None:intergenic 20.0%
!!! TATCATCTAAATGATCATTT+TGG + chr4_3:49459135-49459154 None:intergenic 20.0%
!!! TTTTGCGTTGTTATTTTATT+TGG - chr4_3:49459239-49459258 Msa0645080:CDS 20.0%
!!! TTTGCGTTGTTATTTTATTT+GGG - chr4_3:49459240-49459259 Msa0645080:CDS 20.0%
!!! GTCTCTTTTATTAATCATTT+TGG - chr4_3:49459563-49459582 Msa0645080:intron 20.0%
!! TCAAATCACTATTTAGATTT+AGG - chr4_3:49459589-49459608 Msa0645080:intron 20.0%
!!! ACTATTTAGATTTAGGATAA+TGG - chr4_3:49459596-49459615 Msa0645080:intron 20.0%
!!! CTATTTAGATTTAGGATAAT+GGG - chr4_3:49459597-49459616 Msa0645080:intron 20.0%
!! CATAAACTAATTCATATGTT+AGG - chr4_3:49459751-49459770 Msa0645080:intron 20.0%
!!! CAATATTGATGATATAATTG+AGG - chr4_3:49460428-49460447 Msa0645080:intron 20.0%
!! AAAACTGTAAGAAGTTAAAA+AGG + chr4_3:49460633-49460652 None:intergenic 20.0%
!!! GCTGTTTTAATAAGCTATAT+TGG + chr4_3:49458920-49458939 None:intergenic 25.0%
! TTATCATCAACTTATAGTCA+GGG + chr4_3:49459040-49459059 None:intergenic 25.0%
! ATTATCATCAACTTATAGTC+AGG + chr4_3:49459041-49459060 None:intergenic 25.0%
! GGTTAATACTGAAAATATAG+AGG - chr4_3:49459093-49459112 Msa0645080:intron 25.0%
!!! TTGCGTTGTTATTTTATTTG+GGG - chr4_3:49459241-49459260 Msa0645080:CDS 25.0%
!!! TTATGTTAAAAAAGTATCCC+AGG + chr4_3:49459736-49459755 None:intergenic 25.0%
!!! TTTTAAGTTTGATTAGTCTG+TGG - chr4_3:49459813-49459832 Msa0645080:intron 25.0%
! AAATATTTATTATTGGGTGC+AGG - chr4_3:49459963-49459982 Msa0645080:intron 25.0%
! TGATATAATTGAGGAACAAT+CGG - chr4_3:49460437-49460456 Msa0645080:intron 25.0%
! ATTTATCGATGAATTGTACA+TGG - chr4_3:49460518-49460537 Msa0645080:intron 25.0%
! ACAAGAACATATTCTTTAGA+AGG - chr4_3:49460831-49460850 Msa0645080:intron 25.0%
! TATCAAATTAAACATGAGAG+AGG + chr4_3:49460901-49460920 None:intergenic 25.0%
! TAAAAAATAGAGGACTAAAC+GGG + chr4_3:49461019-49461038 None:intergenic 25.0%
! GTAAAAAATAGAGGACTAAA+CGG + chr4_3:49461020-49461039 None:intergenic 25.0%
! ACTCAATCTGTAAAAAATAG+AGG + chr4_3:49461029-49461048 None:intergenic 25.0%
! AAAATGAGCTGAATCAATTT+AGG + chr4_3:49461072-49461091 None:intergenic 25.0%
TTAGGTTTACGATTTAACAC+GGG + chr4_3:49458563-49458582 None:intergenic 30.0%
GTTAGGTTTACGATTTAACA+CGG + chr4_3:49458564-49458583 None:intergenic 30.0%
ATTCACAACAAACATAGATC+AGG - chr4_3:49458798-49458817 Msa0645080:three_prime_UTR 30.0%
CATAGATCAGGAAAGTTAAA+CGG - chr4_3:49458810-49458829 Msa0645080:three_prime_UTR 30.0%
GTCAATAGCATAGATTCTAT+GGG + chr4_3:49458958-49458977 None:intergenic 30.0%
TGTCAATAGCATAGATTCTA+TGG + chr4_3:49458959-49458978 None:intergenic 30.0%
!! TGTTGATGATATTGAAGATG+TGG - chr4_3:49459177-49459196 Msa0645080:CDS 30.0%
!! GGCATATTTTTACTGAGAAT+CGG - chr4_3:49459262-49459281 Msa0645080:CDS 30.0%
!! GCATATTTTTACTGAGAATC+GGG - chr4_3:49459263-49459282 Msa0645080:CDS 30.0%
GGCGTAAATTTAAAAGTTGA+GGG - chr4_3:49459618-49459637 Msa0645080:intron 30.0%
GCGTAAATTTAAAAGTTGAG+GGG - chr4_3:49459619-49459638 Msa0645080:intron 30.0%
CGTAAATTTAAAAGTTGAGG+GGG - chr4_3:49459620-49459639 Msa0645080:intron 30.0%
ATACAGAGGGAATTAATCAT+TGG + chr4_3:49459680-49459699 None:intergenic 30.0%
! CTTGTGGATGATTATTGATT+AGG - chr4_3:49459841-49459860 Msa0645080:intron 30.0%
! GGATGATTATTGATTAGGAT+CGG - chr4_3:49459846-49459865 Msa0645080:intron 30.0%
CTGATTTATGCACAATCTAT+TGG + chr4_3:49459935-49459954 None:intergenic 30.0%
GTGTATAAGAGAATAAGCAA+CGG - chr4_3:49460091-49460110 Msa0645080:intron 30.0%
AACTTGAATTTGGCTGTAAA+TGG - chr4_3:49460116-49460135 Msa0645080:intron 30.0%
TGCAATAATTGAGAAACAAC+CGG - chr4_3:49460329-49460348 Msa0645080:intron 30.0%
TTACTGACCTCTTTACATAA+TGG + chr4_3:49460369-49460388 None:intergenic 30.0%
GATATAATTGAGGAACAATC+GGG - chr4_3:49460438-49460457 Msa0645080:intron 30.0%
ATATAATTGAGGAACAATCG+GGG - chr4_3:49460439-49460458 Msa0645080:intron 30.0%
AATCCACTGAAGATATGTAA+GGG - chr4_3:49460745-49460764 Msa0645080:intron 30.0%
!!! GCTCATTTTGTCTTGTTTTT+TGG - chr4_3:49461083-49461102 Msa0645080:five_prime_UTR 30.0%
!!! CATTTTGTCTTGTTTTTTGG+CGG - chr4_3:49461086-49461105 Msa0645080:five_prime_UTR 30.0%
!!! ATTTTGTCTTGTTTTTTGGC+GGG - chr4_3:49461087-49461106 Msa0645080:five_prime_UTR 30.0%
AAGTAGCAGATACAACAAGT+TGG - chr4_3:49458615-49458634 Msa0645080:three_prime_UTR 35.0%
AAGGAAAATCCGAACAGAAA+AGG + chr4_3:49458701-49458720 None:intergenic 35.0%
GCTATTGACAGCTTATGTTT+AGG - chr4_3:49458969-49458988 Msa0645080:three_prime_UTR 35.0%
TTAAACAAAAAGAGGCAGCA+AGG + chr4_3:49459331-49459350 None:intergenic 35.0%
TCCATAAATCAGTATGACAG+CGG + chr4_3:49459503-49459522 None:intergenic 35.0%
GGGCGTAAATTTAAAAGTTG+AGG - chr4_3:49459617-49459636 Msa0645080:intron 35.0%
AGTGACTACTTCAATACAGA+GGG + chr4_3:49459693-49459712 None:intergenic 35.0%
AAGTGACTACTTCAATACAG+AGG + chr4_3:49459694-49459713 None:intergenic 35.0%
TTTATACAAACCTATGCTCC+TGG - chr4_3:49459715-49459734 Msa0645080:intron 35.0%
TTATACAAACCTATGCTCCT+GGG - chr4_3:49459716-49459735 Msa0645080:intron 35.0%
! TGTTAGGTCACTTGTTGAAT+AGG - chr4_3:49459767-49459786 Msa0645080:intron 35.0%
! TGATATCCAATAGTGTTGCT+AGG - chr4_3:49459894-49459913 Msa0645080:intron 35.0%
AGCAACGGTAAACTTGAATT+TGG - chr4_3:49460106-49460125 Msa0645080:intron 35.0%
TGAATTTGGCTGTAAATGGA+TGG - chr4_3:49460120-49460139 Msa0645080:intron 35.0%
GAATTTGGCTGTAAATGGAT+GGG - chr4_3:49460121-49460140 Msa0645080:intron 35.0%
TGCTTACTAGAAACTGTTTG+TGG - chr4_3:49460163-49460182 Msa0645080:intron 35.0%
TGAATTGATTGCAGCCAAAT+TGG - chr4_3:49460200-49460219 Msa0645080:intron 35.0%
AGACATATCCTCTTCCAATT+TGG + chr4_3:49460217-49460236 None:intergenic 35.0%
TCTTCAGGATTCATTGAGAA+AGG - chr4_3:49460236-49460255 Msa0645080:intron 35.0%
ATACATGAAACTGAGAACAG+AGG - chr4_3:49460392-49460411 Msa0645080:intron 35.0%
ATCGATGAATTGTACATGGA+TGG - chr4_3:49460522-49460541 Msa0645080:intron 35.0%
! ACAGTTTTTCTTGTCACTGT+AGG - chr4_3:49460645-49460664 Msa0645080:intron 35.0%
AGACAGAAAGTCATAGATTG+CGG - chr4_3:49460694-49460713 Msa0645080:intron 35.0%
CAATCCACTGAAGATATGTA+AGG - chr4_3:49460744-49460763 Msa0645080:intron 35.0%
ATCCACTGAAGATATGTAAG+GGG - chr4_3:49460746-49460765 Msa0645080:intron 35.0%
CTCAGACATTGTTTAGAAAG+AGG - chr4_3:49460858-49460877 Msa0645080:intron 35.0%
AAATATGCATGCAGAGATAG+AGG - chr4_3:49460963-49460982 Msa0645080:intron 35.0%
AGAGATAGAGGTAGCAAAAT+GGG - chr4_3:49460975-49460994 Msa0645080:intron 35.0%
!!! AGCTACTGGGTTTTGTTGTT+AGG + chr4_3:49458581-49458600 None:intergenic 40.0%
AACAACAAAACCCAGTAGCT+CGG - chr4_3:49458581-49458600 Msa0645080:three_prime_UTR 40.0%
! TGTATCTGCTACTTGTTCAG+AGG + chr4_3:49458609-49458628 None:intergenic 40.0%
CATCATCATCATCATCATCC+AGG - chr4_3:49458658-49458677 Msa0645080:three_prime_UTR 40.0%
ACTTATAGTCAGGGTGTGTA+TGG + chr4_3:49459031-49459050 None:intergenic 40.0%
AATCGGGTTTCCAGTTTGAT+AGG - chr4_3:49459279-49459298 Msa0645080:CDS 40.0%
ATCGGGTTTCCAGTTTGATA+GGG - chr4_3:49459280-49459299 Msa0645080:CDS 40.0%
!! TCCGCTGTCATACTGATTTA+TGG - chr4_3:49459499-49459518 Msa0645080:intron 40.0%
!! AAAAAGTATCCCAGGAGCAT+AGG + chr4_3:49459728-49459747 None:intergenic 40.0%
TTAGTCTGTGGATGTTCTTG+TGG - chr4_3:49459825-49459844 Msa0645080:intron 40.0%
CAAAAGCCTAGCAACACTAT+TGG + chr4_3:49459903-49459922 None:intergenic 40.0%
!! CAATAGTGTTGCTAGGCTTT+TGG - chr4_3:49459901-49459920 Msa0645080:intron 40.0%
GTTCAACATTTCCGAGAATG+AGG - chr4_3:49460019-49460038 Msa0645080:intron 40.0%
TGCTATGTTGAAAGAGGAGA+CGG - chr4_3:49460305-49460324 Msa0645080:intron 40.0%
TGCAGGTCCATTATGTAAAG+AGG - chr4_3:49460359-49460378 Msa0645080:intron 40.0%
! TCCAGTTGCATTGACAATAG+AGG - chr4_3:49460467-49460486 Msa0645080:intron 40.0%
ACCTCTATTGTCAATGCAAC+TGG + chr4_3:49460471-49460490 None:intergenic 40.0%
TGTACATGGATGGACAAGAA+AGG - chr4_3:49460532-49460551 Msa0645080:intron 40.0%
!! AAGGTATGCTGCTTTGTATG+TGG - chr4_3:49460551-49460570 Msa0645080:intron 40.0%
TGTATGTGGAGAAGTCTAGT+CGG - chr4_3:49460565-49460584 Msa0645080:intron 40.0%
CTAGTCGGTAAGTTATACAC+TGG - chr4_3:49460580-49460599 Msa0645080:intron 40.0%
CATAGATTGCGGTCAAATTG+TGG - chr4_3:49460705-49460724 Msa0645080:intron 40.0%
TCCACTGAAGATATGTAAGG+GGG - chr4_3:49460747-49460766 Msa0645080:intron 40.0%
TAAGGGGGTAAAAAAAACCC+TGG - chr4_3:49460762-49460781 Msa0645080:intron 40.0%
CTTCATACATCTCATGACCA+GGG + chr4_3:49460782-49460801 None:intergenic 40.0%
ACTTCATACATCTCATGACC+AGG + chr4_3:49460783-49460802 None:intergenic 40.0%
CAGAGATAGAGGTAGCAAAA+TGG - chr4_3:49460974-49460993 Msa0645080:intron 40.0%
TACAGATTGAGTCGAAGTGT+TGG - chr4_3:49461037-49461056 Msa0645080:five_prime_UTR 40.0%
CGAACAGAAAAGGAGAGAAG+GGG + chr4_3:49458691-49458710 None:intergenic 45.0%
CCGAACAGAAAAGGAGAGAA+GGG + chr4_3:49458692-49458711 None:intergenic 45.0%
! CCCTTCTCTCCTTTTCTGTT+CGG - chr4_3:49458689-49458708 Msa0645080:three_prime_UTR 45.0%
TCCGAACAGAAAAGGAGAGA+AGG + chr4_3:49458693-49458712 None:intergenic 45.0%
TTCCTTCCCCTTTCTCGATA+CGG - chr4_3:49458715-49458734 Msa0645080:three_prime_UTR 45.0%
AAGAGTCGACCCTATCAAAC+TGG + chr4_3:49459292-49459311 None:intergenic 45.0%
!! GATAGGGTCGACTCTTTTGA+AGG - chr4_3:49459296-49459315 Msa0645080:CDS 45.0%
GACAGCGGAGTATACAATCA+GGG + chr4_3:49459488-49459507 None:intergenic 45.0%
TGACAGCGGAGTATACAATC+AGG + chr4_3:49459489-49459508 None:intergenic 45.0%
TGAATCCCCTGGTTAGTAGA+TGG - chr4_3:49459649-49459668 Msa0645080:intron 45.0%
TGGGTGCAGGAACAAAATTG+AGG - chr4_3:49459976-49459995 Msa0645080:intron 45.0%
TGTATGTCGCACCTCATTCT+CGG + chr4_3:49460033-49460052 None:intergenic 45.0%
GATTGCAGCCAAATTGGAAG+AGG - chr4_3:49460206-49460225 Msa0645080:intron 45.0%
GGAAGAGGATATGTCTCTTC+AGG - chr4_3:49460221-49460240 Msa0645080:intron 45.0%
AACTGCCCTATTTGAGAACG+AGG - chr4_3:49460275-49460294 Msa0645080:intron 45.0%
GCGAACCTCGTTCTCAAATA+GGG + chr4_3:49460283-49460302 None:intergenic 45.0%
AAACAACCGGAGCATATTGC+AGG - chr4_3:49460342-49460361 Msa0645080:intron 45.0%
AATGGACCTGCAATATGCTC+CGG + chr4_3:49460351-49460370 None:intergenic 45.0%
ACCCCCTTACATATCTTCAG+TGG + chr4_3:49460751-49460770 None:intergenic 45.0%
CATGAGAGAGGACGTGATTT+AGG + chr4_3:49460889-49460908 None:intergenic 45.0%
! GCAAAATGGGTCTGATTCGT+CGG - chr4_3:49460988-49461007 Msa0645080:intron 45.0%
!! TTGAGTCGAAGTGTTGGACT+CGG - chr4_3:49461043-49461062 Msa0645080:five_prime_UTR 45.0%
!!! TTTTGGCGGGCTTAAACATG+TGG - chr4_3:49461100-49461119 Msa0645080:five_prime_UTR 45.0%
!! TTATTCTAAATATTTATTAT+TGG - chr4_3:49459956-49459975 Msa0645080:intron 5.0%
!! TATTCTAAATATTTATTATT+GGG - chr4_3:49459957-49459976 Msa0645080:intron 5.0%
GAGAGAAGGGGAAACAAACC+TGG + chr4_3:49458679-49458698 None:intergenic 50.0%
CTAGTATGCGATTCCATCGC+TGG - chr4_3:49458745-49458764 Msa0645080:three_prime_UTR 50.0%
CGGTTGAGTAGACGACTGTT+TGG - chr4_3:49458830-49458849 Msa0645080:three_prime_UTR 50.0%
!! AGGGTCGACTCTTTTGAAGG+AGG - chr4_3:49459299-49459318 Msa0645080:CDS 50.0%
CAGAGCCATCTACTAACCAG+GGG + chr4_3:49459657-49459676 None:intergenic 50.0%
GCAGAGCCATCTACTAACCA+GGG + chr4_3:49459658-49459677 None:intergenic 50.0%
CGCGAACCTCGTTCTCAAAT+AGG + chr4_3:49460284-49460303 None:intergenic 50.0%
TCGCGCTGCTATGTTGAAAG+AGG - chr4_3:49460299-49460318 Msa0645080:intron 50.0%
GGGCTTAAACATGTGGCTGA+CGG - chr4_3:49461107-49461126 Msa0645080:five_prime_UTR 50.0%
CTTCCCCTTTCTCGATACGG+CGG - chr4_3:49458718-49458737 Msa0645080:three_prime_UTR 55.0%
CCGCATTGAAGGGACGGATA+CGG + chr4_3:49458774-49458793 None:intergenic 55.0%
CCGTATCCGTCCCTTCAATG+CGG - chr4_3:49458771-49458790 Msa0645080:three_prime_UTR 55.0%
GTGAATCGCGCCGCATTGAA+GGG + chr4_3:49458784-49458803 None:intergenic 55.0%
TGTGAATCGCGCCGCATTGA+AGG + chr4_3:49458785-49458804 None:intergenic 55.0%
GGCAGAGCCATCTACTAACC+AGG + chr4_3:49459659-49459678 None:intergenic 55.0%
!! TTCAGAGGCGCCGAGCTACT+GGG + chr4_3:49458594-49458613 None:intergenic 60.0%
CGCCGTATCGAGAAAGGGGA+AGG + chr4_3:49458720-49458739 None:intergenic 60.0%
TGCGCCGCCGTATCGAGAAA+GGG + chr4_3:49458725-49458744 None:intergenic 60.0%
ATCGCGCCGCATTGAAGGGA+CGG + chr4_3:49458780-49458799 None:intergenic 60.0%
!! GTTCAGAGGCGCCGAGCTAC+TGG + chr4_3:49458595-49458614 None:intergenic 65.0%
GCGCCGCCGTATCGAGAAAG+GGG + chr4_3:49458724-49458743 None:intergenic 65.0%
GTGCGCCGCCGTATCGAGAA+AGG + chr4_3:49458726-49458745 None:intergenic 65.0%
ACGGATACGGCAGCCAGCGA+TGG + chr4_3:49458761-49458780 None:intergenic 65.0%
GGGGGTTCGACTGAATCCCC+TGG - chr4_3:49459638-49459657 Msa0645080:intron 65.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 49458554 49461146 49458554 ID=Msa0645080;Name=Msa0645080
chr4_3 mRNA 49458554 49461146 49458554 ID=Msa0645080-mRNA-1;Parent=Msa0645080;Name=Msa0645080-mRNA-1;_AED=0.27;_eAED=0.27;_QI=155|1|1|1|0.66|0.75|4|437|154
chr4_3 exon 49458554 49459033 49458554 ID=Msa0645080-mRNA-1:exon:11831;Parent=Msa0645080-mRNA-1
chr4_3 exon 49459114 49459508 49459114 ID=Msa0645080-mRNA-1:exon:11830;Parent=Msa0645080-mRNA-1
chr4_3 exon 49459660 49459715 49459660 ID=Msa0645080-mRNA-1:exon:11829;Parent=Msa0645080-mRNA-1
chr4_3 exon 49461021 49461146 49461021 ID=Msa0645080-mRNA-1:exon:11828;Parent=Msa0645080-mRNA-1
chr4_3 five_prime_UTR 49461021 49461146 49461021 ID=Msa0645080-mRNA-1:five_prime_utr;Parent=Msa0645080-mRNA-1
chr4_3 five_prime_UTR 49459687 49459715 49459687 ID=Msa0645080-mRNA-1:five_prime_utr;Parent=Msa0645080-mRNA-1
chr4_3 CDS 49459660 49459686 49459660 ID=Msa0645080-mRNA-1:cds;Parent=Msa0645080-mRNA-1
chr4_3 CDS 49459114 49459508 49459114 ID=Msa0645080-mRNA-1:cds;Parent=Msa0645080-mRNA-1
chr4_3 CDS 49458991 49459033 49458991 ID=Msa0645080-mRNA-1:cds;Parent=Msa0645080-mRNA-1
chr4_3 three_prime_UTR 49458554 49458990 49458554 ID=Msa0645080-mRNA-1:three_prime_utr;Parent=Msa0645080-mRNA-1
Gene Sequence

>Msa0645080

ATGAAGTTCAACATTTCCGAGAATGAGCAAATTGATGAATTGATTGCAGCCAAATTGGAAGAGGATATGTCTCTTCAGGATTCATTGAGAAAGGTTCATGACTTGTTTATAACTGCCCTATTTGAGAACGAGGTTCGCGCTGCTATGTTGAAAGAGGAGACGGATGCAATAATTGAGAAACAACCGGAGCATATTGCAGGTCCATTATGTAAAGAGGTCAGTAAAATATACATGAAACTGAGAACAGAGGTCAGTGAATTCTTCAATATTGATGATATAATTGAGGAACAATCGGGGCGTGTTCCAGTTGCATTGACAATAGAGGTCATTAAAACGAACATGAAATTGCGAAAATTTATCGATGAATTGTACATGGATGGACAAGAAAGGTATGCTGCTTTGTATGTGGAGAAGTCTAGTCGGTTAATCAAAGACAAAACAGAAAAGCAAGACAGAAAGTCATAG
Protein sequence

>Msa0645080

MKFNISENEQIDELIAAKLEEDMSLQDSLRKVHDLFITALFENEVRAAMLKEETDAIIEKQPEHIAGPLCKEVSKIYMKLRTEVSEFFNIDDIIEEQSGRVPVALTIEVIKTNMKLRKFIDELYMDGQERYAALYVEKSSRLIKDKTEKQDRKS*