AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0548740

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0548740 MtrunA17_Chr4g0032451 68.939 132 41 0 1 132 23 154 4.22e-61 184
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 55 sgRNAs with CRISPR-Local

Find 158 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAAACGGTAAACTTGAATT+TGG 0.210105 4_1:-43020770 Msa0548740:five_prime_UTR
AACTTGAATTTGGCTGTAAA+TGG 0.254716 4_1:-43020760 Msa0548740:five_prime_UTR
AGCTTCTAGGTTATGTTGTT+AGG 0.284467 4_1:+43022275 None:intergenic
CAAAGCAATAATCATCTATT+AGG 0.291589 4_1:+43019810 None:intergenic
GCCTAATTTCCCCATATCTA+TGG 0.308925 4_1:+43020176 None:intergenic
GAAACAAGCTAAAACAATTT+AGG 0.314281 4_1:+43019882 None:intergenic
TTCAGAGGCGCAGAGCTTCT+AGG 0.315260 4_1:+43022262 None:intergenic
ACAAGAACATATTCTTTAGA+AGG 0.321927 4_1:-43020060 Msa0548740:three_prime_UTR
TTCACAGTTACTTTCTAAAT+AGG 0.323544 4_1:+43020083 None:intergenic
GCCATAGATATGGGGAAATT+AGG 0.328183 4_1:-43020177 Msa0548740:exon
GGAAGAGGATATGTCTCTTC+AGG 0.339040 4_1:-43020659 Msa0548740:exon
TTCCGAGAAGTCCAAGAAAA+AGG 0.340935 4_1:-43020896 Msa0548740:intron
AACATGAGAGGACGTGATTT+AGG 0.341494 4_1:+43020004 None:intergenic
TTAGTGACCTCTTTACATAA+TGG 0.375165 4_1:+43020514 None:intergenic
GAACAACCGGAGCATCTTGC+AGG 0.385771 4_1:-43020538 Msa0548740:CDS
TGCATTAATTGCGGAACAAC+CGG 0.397610 4_1:-43020551 Msa0548740:CDS
AAGACAGAAAGCCATAGATA+TGG 0.404902 4_1:-43020187 Msa0548740:exon
GAAGAGACGGATCATATTCC+AGG 0.409810 4_1:-43020430 Msa0548740:CDS
GCTTCTAGGTTATGTTGTTA+GGG 0.420739 4_1:+43022276 None:intergenic
TGAATTGATTGCAGCCGAAT+TGG 0.427925 4_1:-43020680 Msa0548740:five_prime_UTR
TCTTCAGGATTCATTGAGAA+AGG 0.440370 4_1:-43020644 Msa0548740:CDS
TGCTTACTAGAAACTGTTTG+TGG 0.447204 4_1:-43020717 Msa0548740:five_prime_UTR
TTAGGGATTACCAGTTAAAA+CGG 0.449452 4_1:+43022293 None:intergenic
GGTTCTCTGAGATATTTGTT+TGG 0.454082 4_1:-43020039 Msa0548740:three_prime_UTR
TGAGATATTTGTTTGGAAAG+AGG 0.461787 4_1:-43020032 Msa0548740:three_prime_UTR
AGACATATCCTCTTCCAATT+CGG 0.468675 4_1:+43020666 None:intergenic
AGACAGAAAGCCATAGATAT+GGG 0.478143 4_1:-43020186 Msa0548740:exon
TAGGGATTACCAGTTAAAAC+GGG 0.499942 4_1:+43022294 None:intergenic
AATAGATAGTGGTTAAATTG+TGG 0.507370 4_1:-43020134 Msa0548740:three_prime_UTR
AAGGTTTGCTGCTTTGTATG+TGG 0.524131 4_1:-43020329 Msa0548740:CDS
TGAATTTGGCTGTAAATGGA+TGG 0.525329 4_1:-43020756 Msa0548740:five_prime_UTR
TGCAGGTCCATTATGTAAAG+AGG 0.525512 4_1:-43020521 Msa0548740:CDS
AATGGACCTGCAAGATGCTC+CGG 0.528367 4_1:+43020532 None:intergenic
GCGAGTCGAAGTGTTGGACT+TGG 0.531624 4_1:-43019908 Msa0548740:three_prime_UTR
ATTTGTCAATGAATTGTACA+TGG 0.532546 4_1:-43020362 Msa0548740:CDS
GATTGCAGCCGAATTGGAAG+AGG 0.533785 4_1:-43020674 Msa0548740:five_prime_UTR
TGTACATGGATGGACAAGAA+AGG 0.548031 4_1:-43020348 Msa0548740:CDS
GTCAATGAATTGTACATGGA+TGG 0.549535 4_1:-43020358 Msa0548740:CDS
ATCTAAACAAAGAACGTCGC+AGG 0.557222 4_1:+43019957 None:intergenic
ATGCATGCGAGTCGAAGTGT+TGG 0.568333 4_1:-43019914 Msa0548740:three_prime_UTR
GCGGGCTTAAGCGTGGTGGA+CGG 0.572339 4_1:-43019846 Msa0548740:three_prime_UTR
TGTAGAAGAGAATAAGCAAA+CGG 0.577143 4_1:-43020785 Msa0548740:intron
TGTATCTGCTACTTGTTCAG+AGG 0.584728 4_1:+43022247 None:intergenic
GAATTTGGCTGTAAATGGAT+GGG 0.589101 4_1:-43020755 Msa0548740:five_prime_UTR
TGGGTGTAGGAACAAAATTG+AGG 0.593531 4_1:-43020948 Msa0548740:intron
GTACATGAAAGCGAGAAAAG+AGG 0.599519 4_1:-43020488 Msa0548740:CDS
CATCTATTAGGATAGTAAAG+TGG 0.601538 4_1:+43019822 None:intergenic
ATAGTGGTTAAATTGTGGAC+TGG 0.612000 4_1:-43020129 Msa0548740:three_prime_UTR
AGTATCAAATTAAACATGAG+AGG 0.614190 4_1:+43019992 None:intergenic
TTTGGCGGGCTTAAGCGTGG+TGG 0.622449 4_1:-43019850 Msa0548740:three_prime_UTR
AAGTAGCAGATACAACAAGT+TGG 0.636568 4_1:-43022238 Msa0548740:five_prime_UTR
GGAGATGGATGCATTAATTG+CGG 0.639286 4_1:-43020560 Msa0548740:CDS
TTGTATGTGGAGAAGTCTAG+TGG 0.643539 4_1:-43020316 Msa0548740:CDS
GACAGAAAGCCATAGATATG+GGG 0.651329 4_1:-43020185 Msa0548740:exon
TGTATGTGGAGAAGTCTAGT+GGG 0.662396 4_1:-43020315 Msa0548740:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAATCTTCTATTTAAATTA+TGG + chr4_1:43020453-43020472 None:intergenic 15.0%
!!! TTTTGCATTGTTATTTTATT+TGG - chr4_1:43020486-43020505 Msa0548740:CDS 15.0%
!!! TTTGCATTGTTATTTTATTT+GGG - chr4_1:43020487-43020506 Msa0548740:CDS 15.0%
!! ATTAATAAAAGAGACATTTA+TGG + chr4_1:43020729-43020748 None:intergenic 15.0%
!! TTTGAAAAAAAATGTTTCTT+AGG - chr4_1:43021287-43021306 Msa0548740:intron 15.0%
!!! CAATATTGATAAATTTTTTG+AGG - chr4_1:43021656-43021675 Msa0548740:intron 15.0%
!! TATGCTATAAGCTTAAAATT+AGG - chr4_1:43020245-43020264 Msa0548740:intron 20.0%
!! AAATGAAATTCTTGAAAATG+TGG - chr4_1:43020319-43020338 Msa0548740:CDS 20.0%
!!! ATCATCTAAATGATCATTTT+CGG + chr4_1:43020381-43020400 None:intergenic 20.0%
!!! TTGCATTGTTATTTTATTTG+GGG - chr4_1:43020488-43020507 Msa0548740:CDS 20.0%
!! ATTAAATCTAAATATGACAG+CGG + chr4_1:43020692-43020711 None:intergenic 20.0%
!!! GTCTCTTTTATTAATCATTT+TGG - chr4_1:43020734-43020753 Msa0548740:five_prime_UTR 20.0%
!! TCAAATCACTATTTAGATTT+AGG - chr4_1:43020760-43020779 Msa0548740:five_prime_UTR 20.0%
!! AATTAATCATTAGCAATCAT+TGG + chr4_1:43020851-43020870 None:intergenic 20.0%
!! CATAAACTAATTCATATGTT+AGG - chr4_1:43020931-43020950 Msa0548740:five_prime_UTR 20.0%
!! ATTTATTTATTATTGGGTGT+AGG - chr4_1:43021147-43021166 Msa0548740:intron 20.0%
!!! AAACATTTTTTTTCAAAAGC+AGG + chr4_1:43021284-43021303 None:intergenic 20.0%
!!! GCTGTTTTAATAAGCTATAT+TGG + chr4_1:43020167-43020186 None:intergenic 25.0%
! TTATCATCAACTTATAGTCA+GGG + chr4_1:43020287-43020306 None:intergenic 25.0%
! ATTATCATCAACTTATAGTC+AGG + chr4_1:43020288-43020307 None:intergenic 25.0%
! GGTTAATACTGAAAATATAG+AGG - chr4_1:43020340-43020359 Msa0548740:CDS 25.0%
! CGTGTAAATTTAAAAGTTGA+GGG - chr4_1:43020789-43020808 Msa0548740:intron 25.0%
! GTGTAAATTTAAAAGTTGAG+GGG - chr4_1:43020790-43020809 Msa0548740:intron 25.0%
! TGTAAATTTAAAAGTTGAGG+GGG - chr4_1:43020791-43020810 Msa0548740:intron 25.0%
!!! TTTATGTTAAAAAGTATCCC+AGG + chr4_1:43020917-43020936 None:intergenic 25.0%
!!! TTTTAAGTTTGATTAGTCTG+TGG - chr4_1:43020993-43021012 Msa0548740:intron 25.0%
!!! TAAATTTTTTGAGGAAGAGA+CGG - chr4_1:43021665-43021684 Msa0548740:intron 25.0%
! ATTTGTCAATGAATTGTACA+TGG - chr4_1:43021746-43021765 Msa0548740:intron 25.0%
! AAAACTGTAAGAAGTCAAAA+GGG + chr4_1:43021861-43021880 None:intergenic 25.0%
! AAAAACTGTAAGAAGTCAAA+AGG + chr4_1:43021862-43021881 None:intergenic 25.0%
!!! GTGTTTTTTCAAATAGATAG+TGG - chr4_1:43021963-43021982 Msa0548740:intron 25.0%
!! AATAGATAGTGGTTAAATTG+TGG - chr4_1:43021974-43021993 Msa0548740:intron 25.0%
!! TTCACAGTTACTTTCTAAAT+AGG + chr4_1:43022028-43022047 None:intergenic 25.0%
! ACAAGAACATATTCTTTAGA+AGG - chr4_1:43022048-43022067 Msa0548740:intron 25.0%
! AGTATCAAATTAAACATGAG+AGG + chr4_1:43022119-43022138 None:intergenic 25.0%
! GAAACAAGCTAAAACAATTT+AGG + chr4_1:43022229-43022248 None:intergenic 25.0%
! CAAAGCAATAATCATCTATT+AGG + chr4_1:43022301-43022320 None:intergenic 25.0%
TTAGGGATTACCAGTTAAAA+CGG + chr4_1:43019818-43019837 None:intergenic 30.0%
ATTCACAACAAACATAGATC+AGG - chr4_1:43020050-43020069 Msa0548740:three_prime_UTR 30.0%
CATAGATCAGGAAAGTTAAA+CGG - chr4_1:43020062-43020081 Msa0548740:three_prime_UTR 30.0%
ACATAAGTTGTCAATAGCAT+AGG + chr4_1:43020214-43020233 None:intergenic 30.0%
GCTATTGACAACTTATGTTT+AGG - chr4_1:43020216-43020235 Msa0548740:CDS 30.0%
!! TGTTGATGATATTGAAGATG+TGG - chr4_1:43020424-43020443 Msa0548740:CDS 30.0%
!! GGCATATTTTTACTTAGAGT+CGG - chr4_1:43020509-43020528 Msa0548740:CDS 30.0%
!! GCATATTTTTACTTAGAGTC+GGG - chr4_1:43020510-43020529 Msa0548740:CDS 30.0%
GCGTGTAAATTTAAAAGTTG+AGG - chr4_1:43020788-43020807 Msa0548740:intron 30.0%
! CTTGTGGATGATTATTGATT+AGG - chr4_1:43021021-43021040 Msa0548740:intron 30.0%
! GGATGATTATTGATTAGGAT+CGG - chr4_1:43021026-43021045 Msa0548740:intron 30.0%
CTGATTTATGCACAATCAAT+TGG + chr4_1:43021115-43021134 None:intergenic 30.0%
TGTAGAAGAGAATAAGCAAA+CGG - chr4_1:43021323-43021342 Msa0548740:intron 30.0%
AACTTGAATTTGGCTGTAAA+TGG - chr4_1:43021348-43021367 Msa0548740:intron 30.0%
!! TTACGCTACTATTTTGAAAG+AGG - chr4_1:43021527-43021546 Msa0548740:intron 30.0%
!! TACTATTTTGAAAGAGGAGA+TGG - chr4_1:43021533-43021552 Msa0548740:intron 30.0%
TTAGTGACCTCTTTACATAA+TGG + chr4_1:43021597-43021616 None:intergenic 30.0%
TGAGATATTTGTTTGGAAAG+AGG - chr4_1:43022076-43022095 Msa0548740:intron 30.0%
CATCTATTAGGATAGTAAAG+TGG + chr4_1:43022289-43022308 None:intergenic 30.0%
! ACCAGTTAAAACGGGATTTT+TGG + chr4_1:43019809-43019828 None:intergenic 35.0%
TAGGGATTACCAGTTAAAAC+GGG + chr4_1:43019817-43019836 None:intergenic 35.0%
GCTTCTAGGTTATGTTGTTA+GGG + chr4_1:43019835-43019854 None:intergenic 35.0%
AGCTTCTAGGTTATGTTGTT+AGG + chr4_1:43019836-43019855 None:intergenic 35.0%
AAGTAGCAGATACAACAAGT+TGG - chr4_1:43019870-43019889 Msa0548740:three_prime_UTR 35.0%
GTCAATAGCATAGGTTCTAT+GGG + chr4_1:43020205-43020224 None:intergenic 35.0%
TGTCAATAGCATAGGTTCTA+TGG + chr4_1:43020206-43020225 None:intergenic 35.0%
AGTGACTACTTCAATACAGA+GGG + chr4_1:43020874-43020893 None:intergenic 35.0%
AAGTGACTACTTCAATACAG+AGG + chr4_1:43020875-43020894 None:intergenic 35.0%
! TGTTAGGTCACTTGTTGAAT+AGG - chr4_1:43020947-43020966 Msa0548740:intron 35.0%
! TGATATCCAATAGTGTTGCT+AGG - chr4_1:43021074-43021093 Msa0548740:intron 35.0%
GCAAACGGTAAACTTGAATT+TGG - chr4_1:43021338-43021357 Msa0548740:intron 35.0%
TGAATTTGGCTGTAAATGGA+TGG - chr4_1:43021352-43021371 Msa0548740:intron 35.0%
GAATTTGGCTGTAAATGGAT+GGG - chr4_1:43021353-43021372 Msa0548740:intron 35.0%
TGCTTACTAGAAACTGTTTG+TGG - chr4_1:43021391-43021410 Msa0548740:intron 35.0%
AGACATATCCTCTTCCAATT+CGG + chr4_1:43021445-43021464 None:intergenic 35.0%
TCTTCAGGATTCATTGAGAA+AGG - chr4_1:43021464-43021483 Msa0548740:intron 35.0%
AAAAACCCTAAAAGAGAACG+AGG - chr4_1:43021503-43021522 Msa0548740:intron 35.0%
! GTAAACCTCGTTCTCTTTTA+GGG + chr4_1:43021511-43021530 None:intergenic 35.0%
! ATCTCTATTTTCAATGCACC+TGG + chr4_1:43021699-43021718 None:intergenic 35.0%
GTCAATGAATTGTACATGGA+TGG - chr4_1:43021750-43021769 Msa0548740:intron 35.0%
! ACAGTTTTTCTTGTCACTGT+AGG - chr4_1:43021873-43021892 Msa0548740:intron 35.0%
AAGACAGAAAGCCATAGATA+TGG - chr4_1:43021921-43021940 Msa0548740:intron 35.0%
AGACAGAAAGCCATAGATAT+GGG - chr4_1:43021922-43021941 Msa0548740:intron 35.0%
ATAGTGGTTAAATTGTGGAC+TGG - chr4_1:43021979-43021998 Msa0548740:intron 35.0%
!! GGTTCTCTGAGATATTTGTT+TGG - chr4_1:43022069-43022088 Msa0548740:intron 35.0%
! TGTATCTGCTACTTGTTCAG+AGG + chr4_1:43019864-43019883 None:intergenic 40.0%
CATCATCATCATCATCATCC+AGG - chr4_1:43019910-43019929 Msa0548740:three_prime_UTR 40.0%
CGAACAGAAAAAGAGAGAAG+GGG + chr4_1:43019943-43019962 None:intergenic 40.0%
CCGAACAGAAAAAGAGAGAA+GGG + chr4_1:43019944-43019963 None:intergenic 40.0%
! CCCTTCTCTCTTTTTCTGTT+CGG - chr4_1:43019941-43019960 Msa0548740:three_prime_UTR 40.0%
TCCGAACAGAAAAAGAGAGA+AGG + chr4_1:43019945-43019964 None:intergenic 40.0%
ACTTATAGTCAGGGTGTGTA+TGG + chr4_1:43020278-43020297 None:intergenic 40.0%
TAGAGTTGACCCTATCAAAC+TGG + chr4_1:43020539-43020558 None:intergenic 40.0%
TTAGTCTGTGGATGTTCTTG+TGG - chr4_1:43021005-43021024 Msa0548740:intron 40.0%
CAAAAGCCTAGCAACACTAT+TGG + chr4_1:43021083-43021102 None:intergenic 40.0%
!! CAATAGTGTTGCTAGGCTTT+TGG - chr4_1:43021081-43021100 Msa0548740:intron 40.0%
TGGGTGTAGGAACAAAATTG+AGG - chr4_1:43021160-43021179 Msa0548740:intron 40.0%
TTCCGAGAAGTCCAAGAAAA+AGG - chr4_1:43021212-43021231 Msa0548740:intron 40.0%
! CACCTTTTTCTTGGACTTCT+CGG + chr4_1:43021217-43021236 None:intergenic 40.0%
! TGTATGTCGCACCTTTTTCT+TGG + chr4_1:43021226-43021245 None:intergenic 40.0%
TGAATTGATTGCAGCCGAAT+TGG - chr4_1:43021428-43021447 Msa0548740:intron 40.0%
! CGTAAACCTCGTTCTCTTTT+AGG + chr4_1:43021512-43021531 None:intergenic 40.0%
GGAGATGGATGCATTAATTG+CGG - chr4_1:43021548-43021567 Msa0548740:intron 40.0%
TGCATTAATTGCGGAACAAC+CGG - chr4_1:43021557-43021576 Msa0548740:intron 40.0%
TGCAGGTCCATTATGTAAAG+AGG - chr4_1:43021587-43021606 Msa0548740:intron 40.0%
GTACATGAAAGCGAGAAAAG+AGG - chr4_1:43021620-43021639 Msa0548740:intron 40.0%
TGTACATGGATGGACAAGAA+AGG - chr4_1:43021760-43021779 Msa0548740:intron 40.0%
!! AAGGTTTGCTGCTTTGTATG+TGG - chr4_1:43021779-43021798 Msa0548740:intron 40.0%
TTGTATGTGGAGAAGTCTAG+TGG - chr4_1:43021792-43021811 Msa0548740:intron 40.0%
TGTATGTGGAGAAGTCTAGT+GGG - chr4_1:43021793-43021812 Msa0548740:intron 40.0%
! CTAGTGGGTAAGTTATACAC+TGG - chr4_1:43021808-43021827 Msa0548740:intron 40.0%
GACAGAAAGCCATAGATATG+GGG - chr4_1:43021923-43021942 Msa0548740:intron 40.0%
GCCATAGATATGGGGAAATT+AGG - chr4_1:43021931-43021950 Msa0548740:intron 40.0%
GCCTAATTTCCCCATATCTA+TGG + chr4_1:43021935-43021954 None:intergenic 40.0%
AACATGAGAGGACGTGATTT+AGG + chr4_1:43022107-43022126 None:intergenic 40.0%
ATCTAAACAAAGAACGTCGC+AGG + chr4_1:43022154-43022173 None:intergenic 40.0%
!!! GCTTGTTTCGTCTCGTTTTT+TGG - chr4_1:43022240-43022259 Msa0548740:five_prime_UTR 40.0%
!!! TGTTTCGTCTCGTTTTTTGG+CGG - chr4_1:43022243-43022262 Msa0548740:five_prime_UTR 40.0%
TTCCTTCCCCTTTCTCGATA+CGG - chr4_1:43019967-43019986 Msa0548740:three_prime_UTR 45.0%
ACGGTTGAGTAGACGATGTT+TGG - chr4_1:43020081-43020100 Msa0548740:three_prime_UTR 45.0%
CGGTTGAGTAGACGATGTTT+GGG - chr4_1:43020082-43020101 Msa0548740:three_prime_UTR 45.0%
GACAGCGGAGTATACAATCA+GGG + chr4_1:43020677-43020696 None:intergenic 45.0%
TGACAGCGGAGTATACAATC+AGG + chr4_1:43020678-43020697 None:intergenic 45.0%
TGAATCCCCTGGTTAGTAGA+TGG - chr4_1:43020820-43020839 Msa0548740:intron 45.0%
TTTATACAAGCCTGTGCTCC+TGG - chr4_1:43020896-43020915 Msa0548740:intron 45.0%
TTATACAAGCCTGTGCTCCT+GGG - chr4_1:43020897-43020916 Msa0548740:five_prime_UTR 45.0%
!! AAAAAGTATCCCAGGAGCAC+AGG + chr4_1:43020909-43020928 None:intergenic 45.0%
GGAAGAGGATATGTCTCTTC+AGG - chr4_1:43021449-43021468 Msa0548740:intron 45.0%
GAAGAGACGGATCATATTCC+AGG - chr4_1:43021678-43021697 Msa0548740:intron 45.0%
!!! GTTTCGTCTCGTTTTTTGGC+GGG - chr4_1:43022244-43022263 Msa0548740:five_prime_UTR 45.0%
!! TCTAAATATTTATTTATTAT+TGG - chr4_1:43021140-43021159 Msa0548740:intron 5.0%
!! CTAAATATTTATTTATTATT+GGG - chr4_1:43021141-43021160 Msa0548740:intron 5.0%
GAGAGAAGGGGAAACAAACC+TGG + chr4_1:43019931-43019950 None:intergenic 50.0%
TGAATTGCGCCGCATTGAAG+GGG + chr4_1:43020035-43020054 None:intergenic 50.0%
GTGAATTGCGCCGCATTGAA+GGG + chr4_1:43020036-43020055 None:intergenic 50.0%
TGTGAATTGCGCCGCATTGA+AGG + chr4_1:43020037-43020056 None:intergenic 50.0%
AGTCGGGTCTCCAGTTTGAT+AGG - chr4_1:43020526-43020545 Msa0548740:CDS 50.0%
GTCGGGTCTCCAGTTTGATA+GGG - chr4_1:43020527-43020546 Msa0548740:CDS 50.0%
CAGAGCCATCTACTAACCAG+GGG + chr4_1:43020828-43020847 None:intergenic 50.0%
GCAGAGCCATCTACTAACCA+GGG + chr4_1:43020829-43020848 None:intergenic 50.0%
GATTGCAGCCGAATTGGAAG+AGG - chr4_1:43021434-43021453 Msa0548740:intron 50.0%
AATGGACCTGCAAGATGCTC+CGG + chr4_1:43021579-43021598 None:intergenic 50.0%
ATGCATGCGAGTCGAAGTGT+TGG - chr4_1:43022194-43022213 Msa0548740:intron 50.0%
!!! TTTTTTGGCGGGCTTAAGCG+TGG - chr4_1:43022255-43022274 Msa0548740:five_prime_UTR 50.0%
! TTCAGAGGCGCAGAGCTTCT+AGG + chr4_1:43019849-43019868 None:intergenic 55.0%
CTTCCCCTTTCTCGATACGG+CGG - chr4_1:43019970-43019989 Msa0548740:three_prime_UTR 55.0%
CGAGTATGCGATTCCATCGC+TGG - chr4_1:43019997-43020016 Msa0548740:three_prime_UTR 55.0%
GGCAGAGCCATCTACTAACC+AGG + chr4_1:43020830-43020849 None:intergenic 55.0%
! GAACAACCGGAGCATCTTGC+AGG - chr4_1:43021570-43021589 Msa0548740:intron 55.0%
!! GCGAGTCGAAGTGTTGGACT+TGG - chr4_1:43022200-43022219 Msa0548740:five_prime_UTR 55.0%
CGCCGTATCGAGAAAGGGGA+AGG + chr4_1:43019972-43019991 None:intergenic 60.0%
TGCGCCGCCGTATCGAGAAA+GGG + chr4_1:43019977-43019996 None:intergenic 60.0%
CCGCATTGAAGGGGCGGATA+CGG + chr4_1:43020026-43020045 None:intergenic 60.0%
CCGTATCCGCCCCTTCAATG+CGG - chr4_1:43020023-43020042 Msa0548740:three_prime_UTR 60.0%
ATTGCGCCGCATTGAAGGGG+CGG + chr4_1:43020032-43020051 None:intergenic 60.0%
!! TTTGGCGGGCTTAAGCGTGG+TGG - chr4_1:43022258-43022277 Msa0548740:five_prime_UTR 60.0%
GCGCCGCCGTATCGAGAAAG+GGG + chr4_1:43019976-43019995 None:intergenic 65.0%
GTGCGCCGCCGTATCGAGAA+AGG + chr4_1:43019978-43019997 None:intergenic 65.0%
GGGGGTTCGACTGAATCCCC+TGG - chr4_1:43020809-43020828 Msa0548740:intron 65.0%
! GCGGGCTTAAGCGTGGTGGA+CGG - chr4_1:43022262-43022281 Msa0548740:five_prime_UTR 65.0%
GCGGATACGGCAGCCAGCGA+TGG + chr4_1:43020013-43020032 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 43019806 43022324 43019806 ID=Msa0548740;Name=Msa0548740
chr4_1 mRNA 43019806 43022324 43019806 ID=Msa0548740-mRNA-1;Parent=Msa0548740;Name=Msa0548740-mRNA-1;_AED=0.10;_eAED=0.10;_QI=322|0.66|0.75|1|0.33|0.5|4|387|132
chr4_1 exon 43019806 43020235 43019806 ID=Msa0548740-mRNA-1:exon:11161;Parent=Msa0548740-mRNA-1
chr4_1 exon 43020316 43020802 43020316 ID=Msa0548740-mRNA-1:exon:11160;Parent=Msa0548740-mRNA-1
chr4_1 exon 43020897 43020961 43020897 ID=Msa0548740-mRNA-1:exon:11159;Parent=Msa0548740-mRNA-1
chr4_1 exon 43022199 43022324 43022199 ID=Msa0548740-mRNA-1:exon:11158;Parent=Msa0548740-mRNA-1
chr4_1 five_prime_UTR 43022199 43022324 43022199 ID=Msa0548740-mRNA-1:five_prime_utr;Parent=Msa0548740-mRNA-1
chr4_1 five_prime_UTR 43020897 43020961 43020897 ID=Msa0548740-mRNA-1:five_prime_utr;Parent=Msa0548740-mRNA-1
chr4_1 five_prime_UTR 43020672 43020802 43020672 ID=Msa0548740-mRNA-1:five_prime_utr;Parent=Msa0548740-mRNA-1
chr4_1 CDS 43020316 43020671 43020316 ID=Msa0548740-mRNA-1:cds;Parent=Msa0548740-mRNA-1
chr4_1 CDS 43020193 43020235 43020193 ID=Msa0548740-mRNA-1:cds;Parent=Msa0548740-mRNA-1
chr4_1 three_prime_UTR 43019806 43020192 43019806 ID=Msa0548740-mRNA-1:three_prime_utr;Parent=Msa0548740-mRNA-1
Gene Sequence

>Msa0548740

ATGTCTCTTCAGGATTCATTGAGAAAGGTTAATGACTTGTTTATAAAAACCCTAAAAGAGAACGAGGTTTACGCTACTATTTTGAAAGAGGAGATGGATGCATTAATTGCGGAACAACCGGAGCATCTTGCAGGTCCATTATGTAAAGAGGTCACTAAAATGTACATGAAAGCGAGAAAAGAGGTCAGTGAATTGTACAATATTGATAAATTTTTTGAGGAAGAGACGGATCATATTCCAGGTGCATTGAAAATAGAGATCATTAAATCGTACATGAAATTGCAAAAATTTGTCAATGAATTGTACATGGATGGACAAGAAAGGTTTGCTGCTTTGTATGTGGAGAAGTCTAGTGGGCTAATCAAAGACAAAACAGAAAAGCAAGACAGAAAGCCATAG
Protein sequence

>Msa0548740

MSLQDSLRKVNDLFIKTLKENEVYATILKEEMDALIAEQPEHLAGPLCKEVTKMYMKARKEVSELYNIDKFFEEETDHIPGALKIEIIKSYMKLQKFVNELYMDGQERFAALYVEKSSGLIKDKTEKQDRKP*