AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0645470

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0645470 MtrunA17_Chr4g0033401 78.571 182 12 2 1 158 1 179 1.50e-93 281
Msa0645470 MtrunA17_Chr4g0033321 79.670 182 9 3 1 158 1 178 2.34e-93 280
Msa0645470 MtrunA17_Chr4g0033331 80.769 182 9 2 1 158 5 184 2.92e-89 270
Msa0645470 MtrunA17_Chr4g0033311 85.484 124 18 0 35 158 61 184 1.00e-76 238
Msa0645470 MtrunA17_Chr3g0087191 77.049 122 28 0 35 156 44 165 3.92e-72 214
Msa0645470 MtrunA17_Chr3g0088441 81.452 124 23 0 35 158 60 183 7.28e-72 225
Msa0645470 MtrunA17_Chr6g0482131 82.258 124 22 0 35 158 58 181 6.43e-71 223
Msa0645470 MtrunA17_Chr4g0033341 75.000 136 34 0 35 170 91 226 9.35e-71 223
Msa0645470 MtrunA17_Chr2g0329191 81.452 124 22 1 35 158 56 178 1.25e-70 221
Msa0645470 MtrunA17_Chr2g0329201 82.258 124 22 0 35 158 53 176 1.97e-70 221
Msa0645470 MtrunA17_Chr4g0033191 72.059 136 38 0 35 170 65 200 8.64e-70 220
Msa0645470 MtrunA17_Chr6g0482081 73.529 136 36 0 35 170 58 193 8.29e-69 217
Msa0645470 MtrunA17_Chr4g0033201 70.896 134 39 0 35 168 58 191 1.31e-67 214
Msa0645470 MtrunA17_Chr4g0033271 70.588 136 40 0 35 170 57 192 3.66e-67 213
Msa0645470 MtrunA17_Chr6g0482071 71.324 136 39 0 35 170 59 194 1.52e-65 209
Msa0645470 MtrunA17_Chr4g0033261 77.419 124 28 0 35 158 63 186 1.89e-64 205
Msa0645470 MtrunA17_Chr4g0033231 76.613 124 29 0 35 158 63 186 2.39e-64 205
Msa0645470 MtrunA17_Chr4g0010501 72.581 124 34 0 35 158 46 169 4.47e-62 199
Msa0645470 MtrunA17_Chr4g0006111 70.968 124 35 1 35 158 87 209 4.03e-58 189
Msa0645470 MtrunA17_Chr5g0418871 67.717 127 38 2 35 161 5 128 7.90e-57 181
Msa0645470 MtrunA17_Chr3g0087271 48.421 190 74 3 1 168 4 191 1.21e-55 182
Msa0645470 MtrunA17_Chr4g0006131 61.029 136 52 1 35 170 64 198 2.93e-52 174
Msa0645470 MtrunA17_Chr4g0043091 62.992 127 44 2 35 161 50 173 4.34e-52 173
Msa0645470 MtrunA17_Chr2g0329321 66.935 124 15 1 35 158 60 157 1.82e-51 171
Msa0645470 MtrunA17_Chr3g0087261 58.042 143 57 2 27 168 50 190 4.47e-51 171
Msa0645470 MtrunA17_Chr1g0195611 64.516 124 39 2 35 158 49 167 6.55e-49 165
Msa0645470 MtrunA17_Chr7g0242681 58.824 136 44 4 28 158 52 180 7.83e-49 165
Msa0645470 MtrunA17_Chr1g0195641 62.097 124 42 2 35 158 49 167 1.23e-47 159
Msa0645470 MtrunA17_Chr5g0428831 62.097 124 42 2 35 158 49 167 3.55e-47 160
Msa0645470 MtrunA17_Chr4g0042171 61.290 124 45 2 35 158 46 166 4.76e-47 160
Msa0645470 MtrunA17_Chr7g0242691 60.484 124 46 2 35 158 53 173 2.09e-46 158
Msa0645470 MtrunA17_Chr5g0430451 57.353 136 32 1 35 170 61 170 2.41e-46 158
Msa0645470 MtrunA17_Chr4g0006971 59.091 132 50 3 28 158 41 169 9.77e-46 156
Msa0645470 MtrunA17_Chr3g0090131 56.061 132 55 3 27 157 34 163 1.22e-43 150
Msa0645470 MtrunA17_Chr2g0288071 47.239 163 71 3 5 153 45 206 2.50e-42 148
Msa0645470 MtrunA17_Chr4g0074551 58.065 124 50 2 35 158 72 193 6.42e-42 147
Msa0645470 MtrunA17_Chr3g0090111 54.545 132 57 3 27 157 32 161 1.41e-41 145
Msa0645470 MtrunA17_Chr4g0074561 55.462 119 52 1 35 153 57 174 1.59e-41 145
Msa0645470 MtrunA17_Chr2g0317821 55.303 132 56 3 28 158 46 175 8.83e-41 143
Msa0645470 MtrunA17_Chr2g0288021 50.735 136 65 2 35 170 57 190 1.47e-40 143
Msa0645470 MtrunA17_Chr6g0482941 40.984 183 87 4 4 168 16 195 5.97e-40 141
Msa0645470 MtrunA17_Chr2g0317841 53.788 132 58 3 28 158 46 175 1.19e-39 140
Msa0645470 MtrunA17_Chr6g0482951 40.437 183 88 4 4 168 16 195 1.66e-39 140
Msa0645470 MtrunA17_Chr2g0301231 67.442 86 24 1 81 162 1 86 1.24e-31 117
Msa0645470 MtrunA17_Chr7g0230651 44.037 109 61 0 60 168 18 126 9.16e-29 109
Msa0645470 MtrunA17_Chr7g0234111 45.385 130 63 2 29 157 90 212 1.66e-26 104
Msa0645470 MtrunA17_Chr7g0234131 45.385 130 62 2 29 157 91 212 1.73e-26 104
Msa0645470 MtrunA17_Chr7g0234091 45.902 122 59 1 29 150 91 205 4.52e-26 103
Msa0645470 MtrunA17_Chr7g0227561 35.115 131 58 2 27 156 59 163 1.50e-22 91.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0645470 AT2G44480.4 67.742 124 40 0 35 158 88 211 6.07e-60 191
Msa0645470 AT5G42260.1 61.538 143 54 1 29 170 46 188 3.19e-59 192
Msa0645470 AT5G24550.1 62.121 132 49 1 35 166 54 184 5.52e-59 192
Msa0645470 AT5G44640.1 60.839 143 55 1 29 170 46 188 8.39e-59 191
Msa0645470 AT5G24540.2 62.121 132 49 1 35 166 54 184 1.04e-58 189
Msa0645470 AT2G44480.1 67.742 124 40 0 35 158 57 180 1.47e-58 191
Msa0645470 AT2G44480.3 67.742 124 40 0 35 158 88 211 2.12e-58 191
Msa0645470 AT2G25630.1 61.702 141 53 1 29 168 45 185 2.40e-58 189
Msa0645470 AT5G24540.1 62.121 132 49 1 35 166 54 184 7.44e-58 189
Msa0645470 AT2G44450.1 59.441 143 57 1 29 170 46 188 1.62e-56 185
Msa0645470 AT2G44470.2 57.664 137 54 2 35 171 51 183 2.46e-55 179
Msa0645470 AT2G44470.1 56.115 139 55 2 35 168 51 188 1.43e-54 179
Msa0645470 AT3G60140.2 60.317 126 49 1 35 160 48 172 1.92e-54 179
Msa0645470 AT2G44460.1 61.111 126 48 1 35 160 51 175 3.11e-54 181
Msa0645470 AT2G44470.3 56.115 139 55 2 35 168 51 188 1.66e-53 179
Msa0645470 AT3G60130.1 62.698 126 47 0 35 160 52 177 2.08e-53 177
Msa0645470 AT3G60140.1 60.317 126 49 1 35 160 48 172 3.61e-53 177
Msa0645470 AT1G75940.1 57.246 138 57 2 29 165 52 188 3.31e-52 174
Msa0645470 AT2G44490.1 60.317 126 50 0 35 160 36 161 6.21e-52 174
Msa0645470 AT1G47600.1 59.231 130 51 1 29 158 60 187 1.56e-51 172
Msa0645470 AT1G47600.2 59.231 130 51 1 29 158 60 187 1.70e-51 172
Msa0645470 AT3G09260.1 55.725 131 57 1 35 165 57 186 3.50e-51 171
Msa0645470 AT1G51470.1 58.462 130 52 1 29 158 60 187 8.58e-51 170
Msa0645470 AT1G52400.2 55.797 138 59 2 29 165 54 190 9.97e-51 169
Msa0645470 AT1G52400.3 55.396 139 60 2 29 166 54 191 2.27e-50 169
Msa0645470 AT1G52400.1 55.396 139 60 2 29 166 54 191 2.27e-50 169
Msa0645470 AT1G66280.1 55.072 138 60 2 29 165 51 187 4.39e-50 169
Msa0645470 AT1G66270.1 54.962 131 58 1 35 165 58 187 1.81e-49 167
Msa0645470 AT3G03640.1 56.818 132 57 0 35 166 56 187 4.11e-49 166
Msa0645470 AT1G26560.1 62.097 124 44 2 35 158 54 174 7.40e-49 165
Msa0645470 AT5G28510.1 53.731 134 61 1 35 168 61 193 3.05e-48 164
Msa0645470 AT1G02850.3 62.097 124 42 2 35 158 48 166 1.06e-47 160
Msa0645470 AT5G54570.3 58.065 124 50 1 35 158 52 173 2.03e-47 160
Msa0645470 AT3G21370.1 52.174 138 64 2 29 165 48 184 2.71e-47 161
Msa0645470 AT1G02850.1 62.097 124 42 2 35 158 48 166 2.95e-47 160
Msa0645470 AT1G02850.4 62.097 124 42 2 35 158 48 166 3.57e-47 160
Msa0645470 AT1G02850.5 62.097 124 42 2 35 158 48 166 3.95e-47 160
Msa0645470 AT1G02850.2 62.097 124 42 2 35 158 48 166 4.36e-47 160
Msa0645470 AT5G54570.1 58.065 124 50 1 35 158 52 173 6.52e-47 160
Msa0645470 AT2G32860.2 52.206 136 65 0 35 170 116 251 1.50e-46 160
Msa0645470 AT2G32860.1 52.206 136 65 0 35 170 116 251 1.61e-46 160
Msa0645470 AT3G60120.1 54.887 133 56 2 35 167 36 164 2.24e-46 159
Msa0645470 AT1G66270.2 52.899 138 61 3 29 165 51 185 5.87e-46 157
Msa0645470 AT1G60090.1 55.303 132 52 2 35 166 46 170 9.67e-46 157
Msa0645470 AT1G60090.2 55.303 132 52 2 35 166 46 170 1.12e-45 156
Msa0645470 AT3G62750.7 56.452 124 47 2 35 158 45 161 1.71e-45 153
Msa0645470 AT3G62750.8 56.452 124 47 2 35 158 45 161 1.71e-45 153
Msa0645470 AT3G62750.3 56.452 124 47 2 35 158 71 187 6.09e-45 153
Msa0645470 AT3G62750.4 56.452 124 47 2 35 158 71 187 2.31e-44 152
Msa0645470 AT3G62750.1 56.452 124 47 2 35 158 45 161 3.20e-44 152
Msa0645470 AT3G62750.2 56.452 124 47 2 35 158 71 187 5.54e-44 152
Msa0645470 AT5G26000.2 57.143 119 50 1 41 158 63 181 1.53e-43 150
Msa0645470 AT5G25980.3 57.143 119 50 1 41 158 75 193 2.09e-43 150
Msa0645470 AT5G25980.1 57.143 119 50 1 41 158 75 193 2.09e-43 150
Msa0645470 AT4G27830.1 55.556 126 48 3 35 158 45 164 3.71e-43 150
Msa0645470 AT5G26000.1 57.143 119 50 1 41 158 63 181 4.37e-43 150
Msa0645470 AT5G25980.2 57.143 119 50 1 41 158 75 193 6.28e-43 150
Msa0645470 AT1G51490.1 52.800 125 57 1 29 153 35 157 1.08e-42 147
Msa0645470 AT1G60270.1 54.032 124 51 2 35 158 46 163 1.74e-42 145
Msa0645470 AT4G27820.1 55.645 124 48 2 35 158 45 161 3.37e-42 147
Msa0645470 AT3G62740.2 54.839 124 50 2 35 158 45 162 1.27e-41 144
Msa0645470 AT5G36890.2 53.472 144 61 4 14 156 19 157 1.39e-41 145
Msa0645470 AT5G36890.1 53.472 144 61 4 14 156 19 157 1.44e-41 145
Msa0645470 AT3G62740.1 54.839 124 50 2 35 158 45 162 4.26e-41 144
Msa0645470 AT4G22100.1 51.515 132 57 2 35 166 44 168 1.82e-40 142
Msa0645470 AT3G18080.1 50.000 134 64 2 35 168 61 191 1.90e-40 142
Msa0645470 AT3G18070.4 54.237 118 51 2 35 152 52 166 1.35e-39 138
Msa0645470 AT1G61820.1 50.000 136 67 1 35 170 52 186 2.73e-39 140
Msa0645470 AT3G18070.3 52.459 122 55 2 35 156 52 170 4.50e-39 139
Msa0645470 AT1G45191.5 52.419 124 52 2 35 158 51 167 6.31e-39 137
Msa0645470 AT1G45191.3 52.419 124 52 2 35 158 51 167 7.79e-39 137
Msa0645470 AT3G18070.1 54.237 118 51 2 35 152 52 166 1.76e-38 137
Msa0645470 AT1G45191.2 52.419 124 52 2 35 158 51 167 2.61e-38 137
Msa0645470 AT1G45191.6 52.419 124 52 2 35 158 51 167 2.65e-38 137
Msa0645470 AT1G45191.4 52.419 124 52 2 35 158 51 167 3.12e-38 137
Msa0645470 AT5G48375.1 50.388 129 58 2 41 168 54 177 1.08e-37 134
Msa0645470 AT1G45191.1 50.000 134 52 3 35 158 51 179 4.91e-37 134
Msa0645470 AT1G61810.2 47.059 136 71 1 35 170 55 189 7.33e-37 129
Msa0645470 AT1G61810.4 47.059 136 71 1 35 170 55 189 1.34e-35 128
Msa0645470 AT4G21760.1 45.588 136 72 2 35 170 76 209 2.33e-35 129
Msa0645470 AT4G21760.2 45.588 136 72 2 35 170 76 209 2.80e-35 129
Msa0645470 AT1G61810.3 47.059 136 71 1 35 170 55 189 3.93e-35 129
Msa0645470 AT1G61810.1 47.059 136 71 1 35 170 55 189 4.32e-35 128
Msa0645470 AT2G44480.2 67.949 78 25 0 81 158 1 78 4.46e-35 127
Msa0645470 AT3G60130.3 61.628 86 33 0 75 160 40 125 1.03e-32 121
Msa0645470 AT3G60130.2 61.628 86 33 0 75 160 40 125 1.28e-32 121
Msa0645470 AT5G54570.2 61.538 78 28 1 81 158 1 76 1.11e-28 110
Msa0645470 AT1G60270.2 57.692 78 31 1 81 158 1 76 5.55e-25 98.2
Msa0645470 AT4G27830.2 56.410 78 32 1 81 158 1 76 7.48e-25 99.8
Msa0645470 AT3G62750.6 56.410 78 32 1 81 158 1 76 1.79e-24 98.6
Msa0645470 AT3G62750.5 56.410 78 32 1 81 158 1 76 1.79e-24 98.6
Msa0645470 AT4G27820.2 56.410 78 32 1 81 158 1 76 4.62e-24 97.4
Msa0645470 AT4G27820.3 56.410 78 32 1 81 158 1 76 4.62e-24 97.4
Msa0645470 AT1G61810.5 46.667 90 47 1 81 170 1 89 1.33e-19 85.1

Find 88 sgRNAs with CRISPR-Local

Find 136 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTATTTAATTATAGGTTTA+TGG 0.116590 4_3:-50766411 Msa0645470:intron
TTAATTTATATTACTGAAAA+TGG 0.152248 4_3:-50765857 Msa0645470:intron
GAATCACCGCCGGTGCAATT+TGG 0.245604 4_3:+50766743 None:intergenic
CACCAGGTCGATGCTCTTCT+TGG 0.247168 4_3:-50766772 Msa0645470:three_prime_UTR
AATTGCACCGGCGGTGATTC+TGG 0.263758 4_3:-50766740 Msa0645470:three_prime_UTR
CAACAACCTCATTGATGAAT+TGG 0.285597 4_3:-50767106 Msa0645470:CDS
TTTCACAGGTTGCTTCAAAT+TGG 0.288843 4_3:-50765946 Msa0645470:intron
GTGGAAGAGGACCAAGTATA+TGG 0.292452 4_3:-50767443 Msa0645470:CDS
GAACTTTGCTTCAAAACATT+TGG 0.292593 4_3:-50766872 Msa0645470:CDS
ATCACTTGAGGAATCACTTA+TGG 0.300722 4_3:-50765636 Msa0645470:three_prime_UTR
TGATAATGTGCAACTAAATA+AGG 0.310787 4_3:+50766710 None:intergenic
AAGGGTTATACCGTAAGATT+TGG 0.337309 4_3:-50765399 Msa0645470:three_prime_UTR
ATATATTATCTATATGTAAT+TGG 0.344365 4_3:-50765151 Msa0645470:three_prime_UTR
TTTCAGTAATATAAATTAAA+GGG 0.356229 4_3:+50765861 None:intergenic
TTGGATAATCTCCATTGCTT+AGG 0.366307 4_3:+50766384 None:intergenic
TGGATAATCTCCATTGCTTA+GGG 0.369513 4_3:+50766385 None:intergenic
AGATAACAAACTTGATATAA+AGG 0.370220 4_3:-50766579 Msa0645470:three_prime_UTR
TAAACTGCAGAAAAGATTCC+AGG 0.375034 4_3:-50766903 Msa0645470:intron
CAAACTCTAGAAGATGAATA+TGG 0.381482 4_3:-50767033 Msa0645470:CDS
AACACTGAACCACTGCAAAT+AGG 0.392837 4_3:-50767552 Msa0645470:intron
TATTCATCTTCTAGAGTTTG+AGG 0.401353 4_3:+50767036 None:intergenic
AGAGCTACAGACTTCATGTT+TGG 0.408860 4_3:-50766548 Msa0645470:three_prime_UTR
GTCTCTCAAAAGGGTGTGAT+AGG 0.412344 4_3:-50766638 Msa0645470:three_prime_UTR
AAAGTATCAAGTCTCTCAAA+AGG 0.412668 4_3:-50766648 Msa0645470:three_prime_UTR
ATACTTTGCATGGTCTTTGT+TGG 0.413214 4_3:-50765439 Msa0645470:three_prime_UTR
AGAAAGTCTCGAATTGCTCT+TGG 0.442143 4_3:+50765911 None:intergenic
TGACTCGTATCATCGATATA+AGG 0.447189 4_3:-50767358 Msa0645470:CDS
ATTGATGAATTGGTGGCAAA+TGG 0.451177 4_3:-50767096 Msa0645470:CDS
CAGGGCTGGTGTAAATGTAA+AGG 0.460153 4_3:-50765463 Msa0645470:three_prime_UTR
AGTGAATCTGTTAGGTAGCT+AGG 0.464690 4_3:+50766215 None:intergenic
AAAAGATATCAAAAGCTCTC+AGG 0.470702 4_3:-50765345 Msa0645470:three_prime_UTR
TATTTACATACAATTTCAAA+TGG 0.472751 4_3:+50765173 None:intergenic
AACGAGAAAGTGAATCTGTT+AGG 0.479548 4_3:+50766207 None:intergenic
AGGGCTGGTGTAAATGTAAA+GGG 0.484456 4_3:-50765462 Msa0645470:three_prime_UTR
GCAGCAAGTGAAGGTGGAAG+AGG 0.487766 4_3:-50767456 Msa0645470:CDS
AACACTTACATTTAGACCAA+TGG 0.493905 4_3:+50766052 None:intergenic
TCATTTATTCTATCTTAATG+AGG 0.494555 4_3:-50765585 Msa0645470:three_prime_UTR
TGGGTATGCAAATGGTGAAA+TGG 0.498834 4_3:-50766795 Msa0645470:three_prime_UTR
AAAACATGCGAGCCTTGGTT+AGG 0.500945 4_3:-50766361 Msa0645470:three_prime_UTR
AGTTTACAAAAGAGCAATCA+AGG 0.505341 4_3:-50766325 Msa0645470:three_prime_UTR
AGTCAAGATGGGTATGCAAA+TGG 0.507686 4_3:-50766803 Msa0645470:three_prime_UTR
AAAGGTCATTCCAAATCTTA+CGG 0.508922 4_3:+50765389 None:intergenic
CATCGATATAAGGAAGATGT+TGG 0.511755 4_3:-50767348 Msa0645470:CDS
AGCAAAGTTCTGCATAGTCC+TGG 0.513255 4_3:+50766885 None:intergenic
CAACCTCATTGATGAATTGG+TGG 0.519451 4_3:-50767103 Msa0645470:CDS
TTCATCATTACTTTAACAGA+AGG 0.528077 4_3:-50767588 Msa0645470:CDS
TCATGGACTTACAGTCAAGA+TGG 0.530161 4_3:-50766815 Msa0645470:three_prime_UTR
TGGAAGAGGACCAAGTATAT+GGG 0.531156 4_3:-50767442 Msa0645470:CDS
CTACAGACTTCATGTTTGGA+TGG 0.540670 4_3:-50766544 Msa0645470:intron
TTCAATGAACCTATTTGCAG+TGG 0.547607 4_3:+50767543 None:intergenic
TTGGAGATAGAGTAAAACAT+TGG 0.559088 4_3:-50766853 Msa0645470:exon
TATCCTCAAAAGATCAGTGA+TGG 0.559832 4_3:-50767402 Msa0645470:CDS
AAGTATCAAGTCTCTCAAAA+GGG 0.564962 4_3:-50766647 Msa0645470:three_prime_UTR
ATCAAAAGCTCTCAGGATTG+TGG 0.567379 4_3:-50765338 Msa0645470:three_prime_UTR
GGATAACTTTGAATGAGTCA+TGG 0.567920 4_3:-50766832 Msa0645470:three_prime_UTR
GTGAAAGTATCCCATATACT+TGG 0.569026 4_3:+50767432 None:intergenic
GCAAATGGTGAAATGGCACC+AGG 0.576425 4_3:-50766788 Msa0645470:three_prime_UTR
AAGATCAGTGATGGAAACAA+TGG 0.577375 4_3:-50767393 Msa0645470:CDS
TTGCCACCAATTCATCAATG+AGG 0.580311 4_3:+50767100 None:intergenic
TGGTTGAATCCAAATTGCAC+CGG 0.581033 4_3:-50766752 Msa0645470:three_prime_UTR
GTATCACTCACATGATGAGT+GGG 0.583322 4_3:+50766998 None:intergenic
CATGGACTTACAGTCAAGAT+GGG 0.583758 4_3:-50766814 Msa0645470:three_prime_UTR
TCAAGTTTGTTATCTGAGAG+TGG 0.587930 4_3:+50766587 None:intergenic
AATCATGAAGGATATGAACT+TGG 0.589040 4_3:-50767325 Msa0645470:CDS
GGAAGATGTTGGAATCATGA+AGG 0.590169 4_3:-50767337 Msa0645470:CDS
AACCAAGAAGAGCATCGACC+TGG 0.592148 4_3:+50766770 None:intergenic
TGATCCATCGTTATCACTTG+AGG 0.594621 4_3:-50765648 Msa0645470:three_prime_UTR
AGTGGAGGAATTAATCAAGA+AGG 0.596031 4_3:-50767141 Msa0645470:CDS
GAAGGTGCAGCAAGTGAAGG+TGG 0.597164 4_3:-50767462 Msa0645470:intron
TATGCAGATGGAAAACTAAG+TGG 0.598010 4_3:-50767159 Msa0645470:intron
TTTATGGATCCCCTAAGCAA+TGG 0.602529 4_3:-50766395 Msa0645470:three_prime_UTR
TATGAAGGTGCAGCAAGTGA+AGG 0.604375 4_3:-50767465 Msa0645470:intron
GTGATTGCAGTTGAACGTGA+TGG 0.608566 4_3:-50766080 Msa0645470:intron
TGTATCACTCACATGATGAG+TGG 0.613126 4_3:+50766997 None:intergenic
TTCCGTTCCAGAATCACCGC+CGG 0.613932 4_3:+50766733 None:intergenic
CACCGGCGGTGATTCTGGAA+CGG 0.616221 4_3:-50766735 Msa0645470:three_prime_UTR
GGATAATCTCCATTGCTTAG+GGG 0.619619 4_3:+50766386 None:intergenic
GGGTGTGATAGGTATAACAT+TGG 0.623641 4_3:-50766627 Msa0645470:three_prime_UTR
ATGTAAAGGGATACTTTGCA+TGG 0.626161 4_3:-50765449 Msa0645470:three_prime_UTR
TTGAATCCAAATTGCACCGG+CGG 0.626765 4_3:-50766749 Msa0645470:three_prime_UTR
GGTTTGCCATTGCTCAACTG+AGG 0.628802 4_3:+50766236 None:intergenic
GATGCACCTCAGTTGAGCAA+TGG 0.634368 4_3:-50766242 Msa0645470:three_prime_UTR
GCAGATGGAAAACTAAGTGG+AGG 0.645764 4_3:-50767156 Msa0645470:intron
GAGCAATCAAGGCTAGTGAG+TGG 0.679451 4_3:-50766314 Msa0645470:three_prime_UTR
GATTCCTCAAGTGATAACGA+TGG 0.682607 4_3:+50765644 None:intergenic
ACTCTAGAAGATGAATATGG+CGG 0.692578 4_3:-50767030 Msa0645470:CDS
TGGAAACAATGGAGACGTTG+CGG 0.708998 4_3:-50767382 Msa0645470:CDS
TGGTAATCGTGACCTAACCA+AGG 0.710047 4_3:+50766349 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTATAAATTTAAATTAA+TGG - chr4_3:50766655-50766674 Msa0645470:three_prime_UTR 0.0%
!!! CTCTTTTTTTATTTAATTAT+AGG - chr4_3:50766363-50766382 Msa0645470:three_prime_UTR 10.0%
!! TTTATTTAATTATAGGTTTA+TGG - chr4_3:50766370-50766389 Msa0645470:three_prime_UTR 10.0%
!! TTTCAGTAATATAAATTAAA+GGG + chr4_3:50766923-50766942 None:intergenic 10.0%
!!! TTTTCAGTAATATAAATTAA+AGG + chr4_3:50766924-50766943 None:intergenic 10.0%
!! TTAATTTATATTACTGAAAA+TGG - chr4_3:50766924-50766943 Msa0645470:intron 10.0%
!! ATATATTATCTATATGTAAT+TGG - chr4_3:50767630-50767649 Msa0645470:CDS 10.0%
!! AGATAAACATATAATTGATA+GGG + chr4_3:50765554-50765573 None:intergenic 15.0%
!! TAACTTAGTTTAAATATTTG+TGG - chr4_3:50765826-50765845 Msa0645470:intron 15.0%
!!! AAATTGGTAATTAATGAATT+TGG - chr4_3:50766803-50766822 Msa0645470:three_prime_UTR 15.0%
!!! TTTCTTTTAATCTAAATACA+GGG - chr4_3:50767300-50767319 Msa0645470:CDS 15.0%
!!! AATAATTGTTTTTACAACTA+TGG + chr4_3:50767550-50767569 None:intergenic 15.0%
!! TATTTACATACAATTTCAAA+TGG + chr4_3:50767611-50767630 None:intergenic 15.0%
!! GAGATAAACATATAATTGAT+AGG + chr4_3:50765555-50765574 None:intergenic 20.0%
!!! ATTTGTAACACTTTTTCATT+GGG - chr4_3:50765717-50765736 Msa0645470:intron 20.0%
!! AGATAACAAACTTGATATAA+AGG - chr4_3:50766202-50766221 Msa0645470:three_prime_UTR 20.0%
!!! TTTTCATGTAAGAACATTTT+TGG - chr4_3:50766596-50766615 Msa0645470:three_prime_UTR 20.0%
!! TCAATCAATAGATATAAAGT+AGG + chr4_3:50766622-50766641 None:intergenic 20.0%
!!! TATTTGGTTTTTGTCTAAAT+TGG - chr4_3:50766787-50766806 Msa0645470:three_prime_UTR 20.0%
!! TCATTTATTCTATCTTAATG+AGG - chr4_3:50767196-50767215 Msa0645470:intron 20.0%
!!! CTTTCTTTTAATCTAAATAC+AGG - chr4_3:50767299-50767318 Msa0645470:CDS 20.0%
!! ACCTTTGTAGATTATAAAAA+TGG - chr4_3:50767409-50767428 Msa0645470:CDS 20.0%
!!! ACCATTTTTATAATCTACAA+AGG + chr4_3:50767413-50767432 None:intergenic 20.0%
! AAGACAAGAAGAGAAAAAAT+AGG + chr4_3:50765162-50765181 None:intergenic 25.0%
! TTCATCATTACTTTAACAGA+AGG - chr4_3:50765193-50765212 Msa0645470:three_prime_UTR 25.0%
!! CATACAGATTTTCTATATCT+TGG - chr4_3:50765482-50765501 Msa0645470:intron 25.0%
! ACTTCATTAATTTATGCAGA+TGG - chr4_3:50765610-50765629 Msa0645470:three_prime_UTR 25.0%
!!! CAATGAAAAAGTGTTACAAA+TGG + chr4_3:50765718-50765737 None:intergenic 25.0%
!!! CATTTGTAACACTTTTTCAT+TGG - chr4_3:50765716-50765735 Msa0645470:intron 25.0%
!! TGATAATGTGCAACTAAATA+AGG + chr4_3:50766074-50766093 None:intergenic 25.0%
! TAATCATCCAAATTAACACA+AGG - chr4_3:50766279-50766298 Msa0645470:three_prime_UTR 25.0%
! TATATCTCCTTGTGTTAATT+TGG + chr4_3:50766289-50766308 None:intergenic 25.0%
!!! AGTGGTTCATTTGATTTTAT+TGG - chr4_3:50766485-50766504 Msa0645470:intron 25.0%
!!! TTTGGTTATTTTACTTTCAC+AGG - chr4_3:50766821-50766840 Msa0645470:three_prime_UTR 25.0%
!!! AACTTTTTCATAATGTGTGT+AGG - chr4_3:50767095-50767114 Msa0645470:CDS 25.0%
!! AATTTTGTAGCACTGTTAAA+TGG + chr4_3:50767515-50767534 None:intergenic 25.0%
AATCATGAAGGATATGAACT+TGG - chr4_3:50765456-50765475 Msa0645470:three_prime_UTR 30.0%
TCAATTGACTAGACATACTT+GGG + chr4_3:50765519-50765538 None:intergenic 30.0%
TTCAATTGACTAGACATACT+TGG + chr4_3:50765520-50765539 None:intergenic 30.0%
!! TATTCATCTTCTAGAGTTTG+AGG + chr4_3:50765748-50765767 None:intergenic 30.0%
! CAAACTCTAGAAGATGAATA+TGG - chr4_3:50765748-50765767 Msa0645470:intron 30.0%
GAACTTTGCTTCAAAACATT+TGG - chr4_3:50765909-50765928 Msa0645470:three_prime_UTR 30.0%
TTGGAGATAGAGTAAAACAT+TGG - chr4_3:50765928-50765947 Msa0645470:three_prime_UTR 30.0%
! AAAGTATCAAGTCTCTCAAA+AGG - chr4_3:50766133-50766152 Msa0645470:intron 30.0%
AAGTATCAAGTCTCTCAAAA+GGG - chr4_3:50766134-50766153 Msa0645470:intron 30.0%
! GATTGCTCTTTTGTAAACTT+TGG + chr4_3:50766455-50766474 None:intergenic 30.0%
AGTTTACAAAAGAGCAATCA+AGG - chr4_3:50766456-50766475 Msa0645470:intron 30.0%
AACACTTACATTTAGACCAA+TGG + chr4_3:50766732-50766751 None:intergenic 30.0%
TGAGATATTGGAACATTTCA+TGG + chr4_3:50766762-50766781 None:intergenic 30.0%
CCAAATAACAGATGAGATAT+TGG + chr4_3:50766774-50766793 None:intergenic 30.0%
CCAATATCTCATCTGTTATT+TGG - chr4_3:50766771-50766790 Msa0645470:three_prime_UTR 30.0%
GTCTATTTCACTTTCTTTCA+TGG - chr4_3:50767257-50767276 Msa0645470:intron 30.0%
! TTTTAATCTAAATACAGGGC+TGG - chr4_3:50767304-50767323 Msa0645470:CDS 30.0%
! CTTTGTTGGACAATTTTGAA+TGG - chr4_3:50767356-50767375 Msa0645470:CDS 30.0%
! GACAATTTTGAATGGCATAA+GGG - chr4_3:50767364-50767383 Msa0645470:CDS 30.0%
AAAGGTCATTCCAAATCTTA+CGG + chr4_3:50767395-50767414 None:intergenic 30.0%
AAAAGATATCAAAAGCTCTC+AGG - chr4_3:50767436-50767455 Msa0645470:CDS 30.0%
TTCAATGAACCTATTTGCAG+TGG + chr4_3:50765241-50765260 None:intergenic 35.0%
AATAGAAAAGACTTCCCAGA+AGG - chr4_3:50765259-50765278 Msa0645470:three_prime_UTR 35.0%
! TTCCCAGAAGGCTTTATATT+TGG - chr4_3:50765271-50765290 Msa0645470:three_prime_UTR 35.0%
TGCCAAATATAAAGCCTTCT+GGG + chr4_3:50765276-50765295 None:intergenic 35.0%
TCTTCTGCATATCAGTATGA+AGG - chr4_3:50765301-50765320 Msa0645470:three_prime_UTR 35.0%
!! GTGAAAGTATCCCATATACT+TGG + chr4_3:50765352-50765371 None:intergenic 35.0%
TATCCTCAAAAGATCAGTGA+TGG - chr4_3:50765379-50765398 Msa0645470:three_prime_UTR 35.0%
! TTTCCATCACTGATCTTTTG+AGG + chr4_3:50765385-50765404 None:intergenic 35.0%
! AAGATCAGTGATGGAAACAA+TGG - chr4_3:50765388-50765407 Msa0645470:three_prime_UTR 35.0%
TGACTCGTATCATCGATATA+AGG - chr4_3:50765423-50765442 Msa0645470:three_prime_UTR 35.0%
CATCGATATAAGGAAGATGT+TGG - chr4_3:50765433-50765452 Msa0645470:three_prime_UTR 35.0%
TATGCAGATGGAAAACTAAG+TGG - chr4_3:50765622-50765641 Msa0645470:three_prime_UTR 35.0%
AGTGGAGGAATTAATCAAGA+AGG - chr4_3:50765640-50765659 Msa0645470:three_prime_UTR 35.0%
CAACAACCTCATTGATGAAT+TGG - chr4_3:50765675-50765694 Msa0645470:intron 35.0%
! ATTGATGAATTGGTGGCAAA+TGG - chr4_3:50765685-50765704 Msa0645470:intron 35.0%
! ACTCTAGAAGATGAATATGG+CGG - chr4_3:50765751-50765770 Msa0645470:intron 35.0%
TAAACTGCAGAAAAGATTCC+AGG - chr4_3:50765878-50765897 Msa0645470:three_prime_UTR 35.0%
GGATAACTTTGAATGAGTCA+TGG - chr4_3:50765949-50765968 Msa0645470:intron 35.0%
!! TCAAGTTTGTTATCTGAGAG+TGG + chr4_3:50766197-50766216 None:intergenic 35.0%
TGGATAATCTCCATTGCTTA+GGG + chr4_3:50766399-50766418 None:intergenic 35.0%
TTGGATAATCTCCATTGCTT+AGG + chr4_3:50766400-50766419 None:intergenic 35.0%
AACGAGAAAGTGAATCTGTT+AGG + chr4_3:50766577-50766596 None:intergenic 35.0%
TTTCACAGGTTGCTTCAAAT+TGG - chr4_3:50766835-50766854 Msa0645470:three_prime_UTR 35.0%
ATCACTTGAGGAATCACTTA+TGG - chr4_3:50767145-50767164 Msa0645470:CDS 35.0%
ATGTAAAGGGATACTTTGCA+TGG - chr4_3:50767332-50767351 Msa0645470:CDS 35.0%
ATACTTTGCATGGTCTTTGT+TGG - chr4_3:50767342-50767361 Msa0645470:CDS 35.0%
! GGACAATTTTGAATGGCATA+AGG - chr4_3:50767363-50767382 Msa0645470:CDS 35.0%
AAGGGTTATACCGTAAGATT+TGG - chr4_3:50767382-50767401 Msa0645470:CDS 35.0%
AACACTGAACCACTGCAAAT+AGG - chr4_3:50765229-50765248 Msa0645470:three_prime_UTR 40.0%
GTGCCAAATATAAAGCCTTC+TGG + chr4_3:50765277-50765296 None:intergenic 40.0%
TGGAAGAGGACCAAGTATAT+GGG - chr4_3:50765339-50765358 Msa0645470:three_prime_UTR 40.0%
GGAAGATGTTGGAATCATGA+AGG - chr4_3:50765444-50765463 Msa0645470:three_prime_UTR 40.0%
CAACCTCATTGATGAATTGG+TGG - chr4_3:50765678-50765697 Msa0645470:intron 40.0%
TTGCCACCAATTCATCAATG+AGG + chr4_3:50765684-50765703 None:intergenic 40.0%
GTATCACTCACATGATGAGT+GGG + chr4_3:50765786-50765805 None:intergenic 40.0%
TGTATCACTCACATGATGAG+TGG + chr4_3:50765787-50765806 None:intergenic 40.0%
TCATGGACTTACAGTCAAGA+TGG - chr4_3:50765966-50765985 Msa0645470:intron 40.0%
CATGGACTTACAGTCAAGAT+GGG - chr4_3:50765967-50765986 Msa0645470:intron 40.0%
AGTCAAGATGGGTATGCAAA+TGG - chr4_3:50765978-50765997 Msa0645470:intron 40.0%
TGGGTATGCAAATGGTGAAA+TGG - chr4_3:50765986-50766005 Msa0645470:intron 40.0%
TGGTTGAATCCAAATTGCAC+CGG - chr4_3:50766029-50766048 Msa0645470:intron 40.0%
GGGTGTGATAGGTATAACAT+TGG - chr4_3:50766154-50766173 Msa0645470:intron 40.0%
AGAGCTACAGACTTCATGTT+TGG - chr4_3:50766233-50766252 Msa0645470:three_prime_UTR 40.0%
CTACAGACTTCATGTTTGGA+TGG - chr4_3:50766237-50766256 Msa0645470:three_prime_UTR 40.0%
TTTATGGATCCCCTAAGCAA+TGG - chr4_3:50766386-50766405 Msa0645470:three_prime_UTR 40.0%
GGATAATCTCCATTGCTTAG+GGG + chr4_3:50766398-50766417 None:intergenic 40.0%
AGTGAATCTGTTAGGTAGCT+AGG + chr4_3:50766569-50766588 None:intergenic 40.0%
! GAACGTGATGGAAAAACCAT+TGG - chr4_3:50766713-50766732 Msa0645470:three_prime_UTR 40.0%
AGAAAGTCTCGAATTGCTCT+TGG + chr4_3:50766873-50766892 None:intergenic 40.0%
TGATCCATCGTTATCACTTG+AGG - chr4_3:50767133-50767152 Msa0645470:CDS 40.0%
! GATTCCTCAAGTGATAACGA+TGG + chr4_3:50767140-50767159 None:intergenic 40.0%
! AGGGCTGGTGTAAATGTAAA+GGG - chr4_3:50767319-50767338 Msa0645470:CDS 40.0%
ATCAAAAGCTCTCAGGATTG+TGG - chr4_3:50767443-50767462 Msa0645470:CDS 40.0%
! TATGAAGGTGCAGCAAGTGA+AGG - chr4_3:50765316-50765335 Msa0645470:three_prime_UTR 45.0%
GTGGAAGAGGACCAAGTATA+TGG - chr4_3:50765338-50765357 Msa0645470:three_prime_UTR 45.0%
TGGAAACAATGGAGACGTTG+CGG - chr4_3:50765399-50765418 Msa0645470:three_prime_UTR 45.0%
GCAGATGGAAAACTAAGTGG+AGG - chr4_3:50765625-50765644 Msa0645470:three_prime_UTR 45.0%
AGCAAAGTTCTGCATAGTCC+TGG + chr4_3:50765899-50765918 None:intergenic 45.0%
TTGAATCCAAATTGCACCGG+CGG - chr4_3:50766032-50766051 Msa0645470:intron 45.0%
! GTCTCTCAAAAGGGTGTGAT+AGG - chr4_3:50766143-50766162 Msa0645470:intron 45.0%
TCCAAAAAACATGCGAGCCT+TGG - chr4_3:50766415-50766434 Msa0645470:intron 45.0%
!!! ACCAAGGCTCGCATGTTTTT+TGG + chr4_3:50766419-50766438 None:intergenic 45.0%
AAAACATGCGAGCCTTGGTT+AGG - chr4_3:50766420-50766439 Msa0645470:intron 45.0%
TGGTAATCGTGACCTAACCA+AGG + chr4_3:50766435-50766454 None:intergenic 45.0%
GTGATTGCAGTTGAACGTGA+TGG - chr4_3:50766701-50766720 Msa0645470:three_prime_UTR 45.0%
! CAGGGCTGGTGTAAATGTAA+AGG - chr4_3:50767318-50767337 Msa0645470:CDS 45.0%
!! TAATTAAAAATAATGAAATA+TGG + chr4_3:50765586-50765605 None:intergenic 5.0%
GCAAATGGTGAAATGGCACC+AGG - chr4_3:50765993-50766012 Msa0645470:intron 50.0%
!! AACCAAGAAGAGCATCGACC+TGG + chr4_3:50766014-50766033 None:intergenic 50.0%
GAGCAATCAAGGCTAGTGAG+TGG - chr4_3:50766467-50766486 Msa0645470:intron 50.0%
GATGCACCTCAGTTGAGCAA+TGG - chr4_3:50766539-50766558 Msa0645470:intron 50.0%
GGTTTGCCATTGCTCAACTG+AGG + chr4_3:50766548-50766567 None:intergenic 50.0%
! GAAGGTGCAGCAAGTGAAGG+TGG - chr4_3:50765319-50765338 Msa0645470:three_prime_UTR 55.0%
GCAGCAAGTGAAGGTGGAAG+AGG - chr4_3:50765325-50765344 Msa0645470:three_prime_UTR 55.0%
CACCAGGTCGATGCTCTTCT+TGG - chr4_3:50766009-50766028 Msa0645470:intron 55.0%
GAATCACCGCCGGTGCAATT+TGG + chr4_3:50766041-50766060 None:intergenic 55.0%
! AATTGCACCGGCGGTGATTC+TGG - chr4_3:50766041-50766060 Msa0645470:intron 55.0%
TTCCGTTCCAGAATCACCGC+CGG + chr4_3:50766051-50766070 None:intergenic 55.0%
! CACCGGCGGTGATTCTGGAA+CGG - chr4_3:50766046-50766065 Msa0645470:intron 60.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 50765148 50767655 50765148 ID=Msa0645470;Name=Msa0645470
chr4_3 mRNA 50765148 50767655 50765148 ID=Msa0645470-mRNA-1;Parent=Msa0645470;Name=Msa0645470-mRNA-1;_AED=0.18;_eAED=0.18;_QI=0|0.87|0.77|1|0.75|0.66|9|1113|173
chr4_3 exon 50765148 50765482 50765148 ID=Msa0645470-mRNA-1:exon:12109;Parent=Msa0645470-mRNA-1
chr4_3 exon 50765578 50765686 50765578 ID=Msa0645470-mRNA-1:exon:12108;Parent=Msa0645470-mRNA-1
chr4_3 exon 50765858 50765960 50765858 ID=Msa0645470-mRNA-1:exon:12107;Parent=Msa0645470-mRNA-1
chr4_3 exon 50766061 50766092 50766061 ID=Msa0645470-mRNA-1:exon:12106;Parent=Msa0645470-mRNA-1
chr4_3 exon 50766201 50766418 50766201 ID=Msa0645470-mRNA-1:exon:12105;Parent=Msa0645470-mRNA-1
chr4_3 exon 50766545 50766915 50766545 ID=Msa0645470-mRNA-1:exon:12104;Parent=Msa0645470-mRNA-1
chr4_3 exon 50767009 50767174 50767009 ID=Msa0645470-mRNA-1:exon:12103;Parent=Msa0645470-mRNA-1
chr4_3 exon 50767283 50767480 50767283 ID=Msa0645470-mRNA-1:exon:12102;Parent=Msa0645470-mRNA-1
chr4_3 exon 50767553 50767655 50767553 ID=Msa0645470-mRNA-1:exon:12101;Parent=Msa0645470-mRNA-1
chr4_3 CDS 50767553 50767655 50767553 ID=Msa0645470-mRNA-1:cds;Parent=Msa0645470-mRNA-1
chr4_3 CDS 50767283 50767480 50767283 ID=Msa0645470-mRNA-1:cds;Parent=Msa0645470-mRNA-1
chr4_3 CDS 50767009 50767174 50767009 ID=Msa0645470-mRNA-1:cds;Parent=Msa0645470-mRNA-1
chr4_3 CDS 50766861 50766915 50766861 ID=Msa0645470-mRNA-1:cds;Parent=Msa0645470-mRNA-1
chr4_3 three_prime_UTR 50766545 50766860 50766545 ID=Msa0645470-mRNA-1:three_prime_utr;Parent=Msa0645470-mRNA-1
chr4_3 three_prime_UTR 50766201 50766418 50766201 ID=Msa0645470-mRNA-1:three_prime_utr;Parent=Msa0645470-mRNA-1
chr4_3 three_prime_UTR 50766061 50766092 50766061 ID=Msa0645470-mRNA-1:three_prime_utr;Parent=Msa0645470-mRNA-1
chr4_3 three_prime_UTR 50765858 50765960 50765858 ID=Msa0645470-mRNA-1:three_prime_utr;Parent=Msa0645470-mRNA-1
chr4_3 three_prime_UTR 50765578 50765686 50765578 ID=Msa0645470-mRNA-1:three_prime_utr;Parent=Msa0645470-mRNA-1
chr4_3 three_prime_UTR 50765148 50765482 50765148 ID=Msa0645470-mRNA-1:three_prime_utr;Parent=Msa0645470-mRNA-1
Gene Sequence

>Msa0645470

ATGAGATATTTCCTATTTTTTCTCTTCTTGTCTTTAGTTTCAACATTCATCATTACTTTAACAGAAGGTCAAGAAATAATTAACACTGAACCACTGCAAATAGGTGCAGCAAGTGAAGGTGGAAGAGGACCAAGTATATGGGATACTTTCACTCATAGATATCCTCAAAAGATCAGTGATGGAAACAATGGAGACGTTGCGGTTGACTCGTATCATCGATATAAGGAAGATGTTGGAATCATGAAGGATATGAACTTGGATGCATACAGATTTTCTATATCTTGGTCTAGAATACTCCCAAATGGAAAACTAAGTGGAGGAATTAATCAAGAAGGAATCGACTATTACAACAACCTCATTGATGAATTGGTGGCAAATGGATTACAACCATTTGTAACACTTTTTCATTGGGATCTTCCTCAAACTCTAGAAGATGAATATGGCGGTTTCTTAAGCCCACTCATCATAAAAGATTCCAGGACTATGCAGAACTTTGCTTCAAAACATTTGGAGATAGAGTAA
Protein sequence

>Msa0645470

MRYFLFFLFLSLVSTFIITLTEGQEIINTEPLQIGAASEGGRGPSIWDTFTHRYPQKISDGNNGDVAVDSYHRYKEDVGIMKDMNLDAYRFSISWSRILPNGKLSGGINQEGIDYYNNLIDELVANGLQPFVTLFHWDLPQTLEDEYGGFLSPLIIKDSRTMQNFASKHLEIE*