AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0604250

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0604250 MtrunA17_Chr4g0033401 88.719 523 16 2 1 483 1 520 0.0 939
Msa0604250 MtrunA17_Chr4g0033321 87.954 523 19 3 1 483 1 519 0.0 929
Msa0604250 MtrunA17_Chr4g0033331 82.792 523 47 3 1 483 5 524 0.0 860
Msa0604250 MtrunA17_Chr4g0033311 69.320 515 114 3 6 480 12 522 0.0 750
Msa0604250 MtrunA17_Chr3g0088441 69.215 497 112 2 25 480 25 521 0.0 730
Msa0604250 MtrunA17_Chr2g0329201 64.892 507 130 3 5 471 7 505 0.0 672
Msa0604250 MtrunA17_Chr6g0482131 63.905 507 140 3 7 471 5 510 0.0 669
Msa0604250 MtrunA17_Chr2g0329191 66.250 480 121 2 32 471 29 507 0.0 663
Msa0604250 MtrunA17_Chr4g0033191 63.883 479 133 1 33 471 39 517 0.0 653
Msa0604250 MtrunA17_Chr4g0033201 60.665 511 160 2 1 471 1 510 0.0 648
Msa0604250 MtrunA17_Chr6g0482071 62.353 510 147 3 12 478 9 516 0.0 643
Msa0604250 MtrunA17_Chr4g0033341 60.672 506 153 3 7 471 41 541 0.0 640
Msa0604250 MtrunA17_Chr4g0033271 61.111 504 155 2 9 471 6 509 0.0 640
Msa0604250 MtrunA17_Chr6g0482081 62.327 507 144 3 6 471 11 511 0.0 629
Msa0604250 MtrunA17_Chr2g0329321 59.100 511 140 4 1 471 5 486 0.0 615
Msa0604250 MtrunA17_Chr4g0033261 56.725 513 151 5 2 471 7 491 0.0 573
Msa0604250 MtrunA17_Chr4g0033231 56.287 501 149 4 13 471 19 491 0.0 561
Msa0604250 MtrunA17_Chr5g0430451 56.867 517 147 4 4 471 7 496 0.0 558
Msa0604250 MtrunA17_Chr4g0010501 56.723 476 160 4 36 471 23 492 0.0 552
Msa0604250 MtrunA17_Chr4g0006111 51.793 502 162 8 22 471 50 523 1.97e-173 498
Msa0604250 MtrunA17_Chr3g0087261 50.000 506 206 6 7 471 8 507 5.58e-173 496
Msa0604250 MtrunA17_Chr3g0087271 47.945 511 220 5 1 471 4 508 2.57e-165 477
Msa0604250 MtrunA17_Chr4g0043091 47.826 506 215 6 10 471 1 501 5.49e-164 473
Msa0604250 MtrunA17_Chr4g0006131 52.263 486 153 10 37 471 42 499 3.57e-163 471
Msa0604250 MtrunA17_Chr4g0042171 46.365 509 222 7 13 478 5 505 2.98e-149 436
Msa0604250 MtrunA17_Chr6g0482951 44.618 511 230 8 3 472 13 511 1.04e-148 434
Msa0604250 MtrunA17_Chr6g0482941 44.814 511 229 8 3 472 13 511 1.95e-148 434
Msa0604250 MtrunA17_Chr3g0090131 44.581 489 217 6 30 477 14 489 1.32e-139 410
Msa0604250 MtrunA17_Chr3g0090111 44.906 481 211 6 37 477 19 485 2.91e-139 409
Msa0604250 MtrunA17_Chr2g0301231 52.278 417 122 7 105 471 1 390 2.72e-137 401
Msa0604250 MtrunA17_Chr4g0074561 44.699 481 209 6 42 473 40 512 4.50e-136 402
Msa0604250 MtrunA17_Chr2g0288071 45.168 476 216 5 46 479 76 548 1.39e-133 397
Msa0604250 MtrunA17_Chr4g0074551 44.280 472 217 6 42 471 55 522 2.08e-131 392
Msa0604250 MtrunA17_Chr2g0317821 42.536 489 226 9 37 478 32 512 1.38e-128 384
Msa0604250 MtrunA17_Chr2g0317841 39.695 524 253 11 2 478 5 512 6.93e-128 382
Msa0604250 MtrunA17_Chr7g0242681 43.595 484 202 10 37 471 38 499 2.86e-124 372
Msa0604250 MtrunA17_Chr2g0288021 40.117 511 257 8 4 472 5 508 6.85e-123 369
Msa0604250 MtrunA17_Chr4g0006971 42.708 480 219 8 36 473 26 491 1.40e-122 368
Msa0604250 MtrunA17_Chr7g0242691 41.632 478 223 7 36 471 30 493 8.35e-122 366
Msa0604250 MtrunA17_Chr1g0195611 41.650 509 222 11 10 471 7 487 3.25e-120 362
Msa0604250 MtrunA17_Chr5g0428831 41.373 510 223 11 10 471 7 488 1.17e-118 357
Msa0604250 MtrunA17_Chr3g0087201 49.245 331 123 3 181 471 17 342 1.05e-108 326
Msa0604250 MtrunA17_Chr5g0418871 53.846 299 95 3 56 314 2 297 3.62e-108 324
Msa0604250 MtrunA17_Chr7g0234111 40.377 478 225 7 38 474 76 534 6.08e-107 328
Msa0604250 MtrunA17_Chr7g0234091 39.212 482 231 6 38 477 76 537 7.37e-106 325
Msa0604250 MtrunA17_Chr7g0234131 39.167 480 231 7 38 475 76 536 1.62e-103 319
Msa0604250 MtrunA17_Chr1g0195641 44.246 391 157 7 39 383 29 404 6.57e-100 306
Msa0604250 MtrunA17_Chr3g0087191 77.241 145 33 0 36 180 21 165 7.66e-82 251
Msa0604250 MtrunA17_Chr7g0230651 38.158 380 171 6 83 418 17 376 2.45e-79 252
Msa0604250 MtrunA17_Chr7g0227561 39.916 238 102 5 39 272 48 248 3.27e-51 176
Msa0604250 MtrunA17_Chr7g0236731 40.800 250 101 3 125 333 27 270 2.07e-44 157
Msa0604250 MtrunA17_Chr4g0068871 26.396 394 227 11 101 473 183 534 1.42e-27 116
Msa0604250 MtrunA17_Chr5g0418861 50.549 91 44 1 383 472 1 91 4.77e-24 95.9
Msa0604250 MtrunA17_Chr8g0352881 55.556 54 23 1 46 99 7 59 1.29e-13 68.6
Msa0604250 MtrunA17_Chr7g0227591 52.727 55 26 0 420 474 14 68 1.97e-13 65.5
Msa0604250 MtrunA17_Chr2g0288031 48.276 58 29 1 189 246 4 60 1.54e-11 59.7
Msa0604250 MtrunA17_Chr2g0301221 71.429 35 10 0 42 76 70 104 5.29e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0604250 AT5G44640.1 50.491 509 204 6 9 476 5 506 0.0 523
Msa0604250 AT5G42260.1 51.297 501 196 6 8 467 4 497 0.0 519
Msa0604250 AT2G44450.1 51.129 487 193 3 25 471 19 500 0.0 518
Msa0604250 AT2G44480.1 50.833 480 195 2 36 475 34 512 7.77e-177 506
Msa0604250 AT3G60130.1 51.471 476 185 4 37 471 30 500 1.18e-176 506
Msa0604250 AT2G44480.3 50.833 480 195 2 36 475 65 543 2.75e-176 506
Msa0604250 AT5G24550.1 47.500 520 226 6 8 481 1 519 3.60e-171 493
Msa0604250 AT2G25630.1 48.532 511 191 6 2 471 4 483 1.12e-170 490
Msa0604250 AT5G24540.1 47.885 520 224 6 8 481 1 519 1.92e-168 486
Msa0604250 AT3G60140.1 46.322 503 219 7 12 471 8 502 5.04e-163 473
Msa0604250 AT2G44460.1 45.489 521 231 6 1 479 3 512 1.20e-160 468
Msa0604250 AT2G44470.3 43.689 515 236 5 1 472 3 506 7.19e-159 463
Msa0604250 AT1G51470.1 49.061 479 190 7 36 472 44 510 2.46e-156 454
Msa0604250 AT1G26560.1 48.340 482 199 7 37 473 32 508 4.56e-156 453
Msa0604250 AT2G44490.1 47.010 485 213 5 36 476 13 497 6.20e-155 452
Msa0604250 AT3G60120.1 47.609 481 203 7 36 471 13 489 1.41e-154 451
Msa0604250 AT1G47600.1 48.643 479 192 7 36 472 44 510 3.42e-154 449
Msa0604250 AT1G47600.2 48.643 479 191 7 36 472 44 509 6.50e-154 448
Msa0604250 AT3G18080.1 46.307 501 217 9 20 476 20 512 2.43e-151 441
Msa0604250 AT5G54570.1 45.155 485 209 5 36 471 29 505 2.59e-147 432
Msa0604250 AT5G25980.2 45.591 533 232 12 1 483 12 536 2.26e-146 430
Msa0604250 AT3G09260.1 45.816 478 216 5 37 472 35 511 1.98e-145 427
Msa0604250 AT3G18070.3 46.107 488 203 10 32 476 25 495 2.29e-145 426
Msa0604250 AT3G18070.1 46.311 488 208 10 32 476 25 501 1.26e-144 424
Msa0604250 AT3G60130.2 49.517 414 163 4 99 471 40 448 1.73e-143 419
Msa0604250 AT1G75940.1 44.990 489 217 8 37 476 37 522 4.62e-143 421
Msa0604250 AT2G44480.2 48.418 411 171 2 105 475 1 410 1.99e-142 415
Msa0604250 AT5G36890.2 46.042 480 206 5 38 476 17 484 5.64e-140 411
Msa0604250 AT5G36890.1 46.042 480 206 5 38 476 17 484 5.95e-140 411
Msa0604250 AT5G24540.2 46.374 455 198 5 8 417 1 454 4.47e-139 408
Msa0604250 AT1G52400.3 43.053 511 242 10 6 471 9 515 7.01e-139 410
Msa0604250 AT1G52400.1 43.053 511 242 10 6 471 9 515 7.01e-139 410
Msa0604250 AT5G28510.1 43.595 484 225 6 37 473 39 521 6.73e-138 408
Msa0604250 AT3G03640.1 42.074 511 246 6 7 471 6 512 7.27e-138 407
Msa0604250 AT3G21370.1 43.776 482 223 8 36 471 32 511 9.07e-138 407
Msa0604250 AT5G26000.1 43.907 517 230 10 7 471 3 511 8.61e-137 405
Msa0604250 AT3G60130.3 48.551 414 156 5 99 471 40 437 1.44e-136 401
Msa0604250 AT2G44470.1 42.048 459 213 4 1 417 3 450 1.85e-135 399
Msa0604250 AT2G32860.2 41.372 481 239 3 36 473 93 573 3.49e-135 404
Msa0604250 AT1G66280.1 43.000 500 239 7 16 471 13 510 3.53e-135 400
Msa0604250 AT2G32860.1 41.250 480 238 4 37 473 94 572 4.79e-134 400
Msa0604250 AT1G66270.1 43.515 478 226 6 37 472 36 511 2.66e-133 395
Msa0604250 AT1G02850.1 47.098 448 207 9 38 471 27 458 5.87e-131 388
Msa0604250 AT1G66270.2 43.305 478 225 7 37 472 36 509 3.26e-130 388
Msa0604250 AT1G61820.1 43.265 490 230 8 29 474 22 507 3.45e-128 382
Msa0604250 AT1G02850.2 44.421 475 207 8 38 471 27 485 3.98e-128 382
Msa0604250 AT3G60140.2 44.552 413 179 6 12 382 8 412 1.61e-126 376
Msa0604250 AT5G25980.3 45.043 464 201 9 1 418 12 467 1.67e-125 374
Msa0604250 AT5G25980.1 45.043 464 201 9 1 418 12 467 1.67e-125 374
Msa0604250 AT5G54570.3 43.619 431 187 4 36 418 29 451 2.91e-124 370
Msa0604250 AT4G21760.2 39.726 511 253 9 6 471 21 521 2.66e-120 363
Msa0604250 AT4G27830.1 40.119 506 232 10 11 471 5 484 6.77e-120 361
Msa0604250 AT1G60090.2 40.612 490 225 11 37 482 24 491 9.00e-118 355
Msa0604250 AT1G02850.5 42.000 500 206 12 38 471 27 508 1.96e-117 355
Msa0604250 AT5G26000.2 42.641 462 207 8 7 418 3 456 5.23e-117 352
Msa0604250 AT1G61810.1 41.975 486 233 9 42 482 38 519 6.72e-117 353
Msa0604250 AT1G02850.4 42.116 501 205 13 38 471 27 509 6.75e-116 351
Msa0604250 AT2G44480.4 54.487 312 100 3 36 307 65 374 8.27e-116 346
Msa0604250 AT2G44470.2 41.235 405 185 4 1 363 3 396 3.27e-115 345
Msa0604250 AT2G44450.2 47.175 354 142 3 158 471 1 349 1.74e-114 342
Msa0604250 AT5G54570.2 43.029 416 180 5 105 471 1 408 4.55e-114 343
Msa0604250 AT4G22100.1 41.213 478 219 10 38 471 23 482 5.01e-114 346
Msa0604250 AT1G52400.2 41.447 456 219 9 6 417 9 460 6.13e-114 344
Msa0604250 AT1G60090.1 39.400 500 224 12 37 482 24 498 9.66e-114 345
Msa0604250 AT4G21760.1 38.748 511 248 10 6 471 21 511 2.70e-113 345
Msa0604250 AT1G61810.3 42.733 461 216 8 42 458 38 494 1.68e-112 343
Msa0604250 AT4G27820.1 41.042 480 220 8 36 471 22 482 1.58e-111 339
Msa0604250 AT3G62750.1 39.184 490 226 9 26 471 12 473 2.74e-111 338
Msa0604250 AT3G62750.2 39.184 490 226 9 26 471 38 499 7.35e-111 338
Msa0604250 AT1G45191.4 40.586 478 216 10 38 471 30 483 1.08e-109 335
Msa0604250 AT1G45191.6 39.220 487 221 10 38 471 30 494 5.84e-106 325
Msa0604250 AT1G45191.2 39.331 478 228 10 38 471 30 489 1.27e-105 324
Msa0604250 AT1G45191.1 38.833 497 221 11 38 471 30 506 1.28e-104 322
Msa0604250 AT3G62740.1 37.838 481 234 11 34 471 20 478 6.75e-104 320
Msa0604250 AT5G48375.1 42.282 447 146 10 33 436 34 411 1.92e-99 306
Msa0604250 AT2G44460.2 43.989 366 163 4 156 479 1 366 3.38e-98 303
Msa0604250 AT1G51490.1 46.110 347 140 6 44 352 27 364 6.92e-98 301
Msa0604250 AT3G18070.4 46.970 330 128 6 32 321 25 347 1.00e-97 300
Msa0604250 AT1G02850.3 42.683 410 158 10 38 382 27 424 3.47e-97 300
Msa0604250 AT1G60270.1 44.477 344 140 6 37 337 24 359 8.73e-95 292
Msa0604250 AT3G62750.4 38.356 438 199 8 26 420 38 447 1.06e-93 292
Msa0604250 AT4G27830.2 38.780 410 194 6 105 471 1 396 2.53e-90 282
Msa0604250 AT3G62740.2 37.793 426 201 10 34 417 20 423 1.34e-89 280
Msa0604250 AT1G61810.5 39.716 423 206 9 105 482 1 419 1.59e-89 280
Msa0604250 AT1G61810.4 41.299 385 179 7 42 382 38 419 4.60e-89 279
Msa0604250 AT1G45191.5 44.048 336 136 6 38 330 30 356 4.21e-88 276
Msa0604250 AT1G45191.3 44.048 336 136 6 38 330 30 356 4.34e-88 276
Msa0604250 AT3G62750.7 42.042 333 137 5 26 318 12 328 4.26e-86 269
Msa0604250 AT3G62750.8 42.042 333 137 5 26 318 12 328 4.26e-86 269
Msa0604250 AT3G62750.3 42.042 333 137 5 26 318 38 354 3.65e-85 269
Msa0604250 AT4G27820.2 39.173 411 192 7 105 471 1 397 1.33e-82 262
Msa0604250 AT4G27820.3 39.173 411 192 7 105 471 1 397 1.33e-82 262
Msa0604250 AT3G18070.2 42.470 332 142 7 188 476 99 424 7.50e-81 257
Msa0604250 AT3G18070.2 58.824 68 27 1 32 99 25 91 3.60e-18 87.0
Msa0604250 AT2G44480.5 42.715 302 132 2 214 475 6 306 1.21e-80 253
Msa0604250 AT1G61810.2 54.113 231 104 2 42 272 38 266 3.62e-80 252
Msa0604250 AT3G62750.6 36.740 411 193 8 105 471 1 388 8.78e-80 254
Msa0604250 AT3G62750.5 36.740 411 193 8 105 471 1 388 8.78e-80 254
Msa0604250 AT1G61820.3 39.946 368 174 7 151 474 4 368 1.80e-79 252
Msa0604250 AT3G18070.7 41.509 318 137 7 202 476 1 312 1.11e-73 235
Msa0604250 AT1G60270.2 42.391 276 112 5 105 337 1 272 2.85e-65 213
Msa0604250 AT3G18070.5 38.397 237 102 4 189 383 100 334 7.46e-47 166
Msa0604250 AT3G18070.5 58.824 68 27 1 32 99 25 91 1.70e-18 87.0
Msa0604250 AT3G18070.6 36.607 224 98 4 202 383 1 222 4.46e-40 144
Msa0604250 AT5G16580.1 30.745 322 154 7 152 428 2 299 4.08e-31 122
Msa0604250 AT3G06510.2 26.471 408 245 13 100 473 159 545 4.85e-30 124
Msa0604250 AT3G06510.1 26.582 395 227 13 100 473 159 511 3.86e-29 121

Find 88 sgRNAs with CRISPR-Local

Find 177 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGATTTATATTACTGAAAA+TGG 0.156629 4_2:-49703615 Msa0604250:intron
TTCCCAGAAGGCTTTATATT+TGG 0.203107 4_2:-49705537 Msa0604250:CDS
GAATCACCGCCGGTGCAATT+TGG 0.245604 4_2:+49704769 None:intergenic
CACCAGGTCGATGCTCTTCT+TGG 0.247168 4_2:-49704798 Msa0604250:CDS
AATTGCACCGGCGGTGATTC+TGG 0.263758 4_2:-49704766 Msa0604250:CDS
CAACAACCTCATTGATGAAT+TGG 0.285597 4_2:-49705133 Msa0604250:CDS
GTGGAAGAGGACCAAGTATA+TGG 0.292452 4_2:-49705470 Msa0604250:CDS
GAACTTTGCTTCAAAACATT+TGG 0.292593 4_2:-49704898 Msa0604250:CDS
ATCACTTGAGGAATCACTTA+TGG 0.300722 4_2:-49703428 Msa0604250:CDS
ATTTCCAGGTTGCTTCAAAT+TGG 0.303894 4_2:-49703704 Msa0604250:intron
TGATAATGTGCAACTAAATA+AGG 0.310787 4_2:+49704736 None:intergenic
GTGCCAAATATAAAGCCTTC+TGG 0.324054 4_2:+49705534 None:intergenic
AATAATTGTTATTACAACTA+TGG 0.341149 4_2:+49703026 None:intergenic
AAGGGTTATACTGTCAGATT+TGG 0.344341 4_2:-49703191 Msa0604250:CDS
AATGTTGTAGCACTGTTAAA+TGG 0.349883 4_2:+49703061 None:intergenic
AAACTGCAGAAAAGATTTCC+AGG 0.361097 4_2:-49704929 Msa0604250:intron
CAAACTCTGGAAGATGAATA+TGG 0.365046 4_2:-49705060 Msa0604250:CDS
ATATAATCTCTATATGTAAT+TGG 0.368988 4_2:-49702943 Msa0604250:three_prime_UTR
AAGACAAGAAGAGAATAAAT+AGG 0.370429 4_2:+49705649 None:intergenic
AACACTGAATCACTGCAAAT+AGG 0.401235 4_2:-49705579 Msa0604250:CDS
AAAAGATATCAAAAGCTCTC+AGG 0.405939 4_2:-49703137 Msa0604250:CDS
TGACTCGTATCATCGCTATA+AGG 0.410464 4_2:-49705385 Msa0604250:CDS
TGCCAAATATAAAGCCTTCT+GGG 0.411107 4_2:+49705535 None:intergenic
GTCTCTCAAAAGGGTGTGAT+AGG 0.412344 4_2:-49704664 Msa0604250:intron
AAAGTATCAAGTCTCTCAAA+AGG 0.412668 4_2:-49704674 Msa0604250:CDS
ATACTTTGCATGGTCTTTGT+TGG 0.413214 4_2:-49703231 Msa0604250:CDS
TTGGGATCTTCCTCAAACTC+TGG 0.415319 4_2:-49705073 Msa0604250:CDS
AGAAAGTCTCGAATTGCTCT+TGG 0.442143 4_2:+49703669 None:intergenic
ATTGATGAATTGGTGGCAAA+TGG 0.451177 4_2:-49705123 Msa0604250:CDS
TGTTCTTACCAAGGCTCTCA+TGG 0.452605 4_2:+49704109 None:intergenic
CAGGGCTGGTGTAAATGTAA+AGG 0.460153 4_2:-49703255 Msa0604250:CDS
TTTCAGTAATATAAATCAAA+GGG 0.466034 4_2:+49703619 None:intergenic
AGAGAATCTGTAAGGTAGCT+AGG 0.466863 4_2:+49703966 None:intergenic
TATTTACATACAATTTCAAA+TGG 0.472751 4_2:+49702963 None:intergenic
TATTCATCTTCCAGAGTTTG+AGG 0.478589 4_2:+49705063 None:intergenic
AGGGCTGGTGTAAATGTAAA+GGG 0.484456 4_2:-49703254 Msa0604250:CDS
GCAGCAAGTGAAGGTGGAAG+AGG 0.487766 4_2:-49705483 Msa0604250:CDS
AACACTTACATTTAGACCAA+TGG 0.493905 4_2:+49703808 None:intergenic
TCATTTATTCTATCTTAATG+AGG 0.494555 4_2:-49703377 Msa0604250:CDS
TGGGTATGCAAATGGTGAAA+TGG 0.498834 4_2:-49704821 Msa0604250:CDS
CCATGGACTTACAGTCAAGA+TGG 0.501018 4_2:-49704841 Msa0604250:CDS
ATCAAAAGCTCTCAGGGTTG+TGG 0.504177 4_2:-49703130 Msa0604250:CDS
AGTTTACAAAAGAGCAATCA+AGG 0.505341 4_2:-49704076 Msa0604250:CDS
AGCAAAGTTCTGCATAGTCC+TGG 0.506435 4_2:+49704911 None:intergenic
AGTCAAGATGGGTATGCAAA+TGG 0.507686 4_2:-49704829 Msa0604250:CDS
CAACCTCATTGATGAATTGG+TGG 0.519451 4_2:-49705130 Msa0604250:CDS
CATCGCTATAAGGAAGATGT+TGG 0.525320 4_2:-49705375 Msa0604250:CDS
TTCATCATTACTTTAACAGA+AGG 0.528077 4_2:-49705615 Msa0604250:CDS
AGAATGAATGAATGAATGAA+TGG 0.530411 4_2:+49705780 None:intergenic
TGGAAGAGGACCAAGTATAT+GGG 0.531156 4_2:-49705469 Msa0604250:CDS
AACGAGAAAGAGAATCTGTA+AGG 0.533045 4_2:+49703958 None:intergenic
AAAATCACTGATGGAAACAA+TGG 0.550597 4_2:-49705420 Msa0604250:CDS
TTGGAGATAGAGTAAAACAT+TGG 0.559088 4_2:-49704879 Msa0604250:CDS
TCTTCTGCATATCAGTATGA+AGG 0.560527 4_2:-49705507 Msa0604250:CDS
AAGTATCAAGTCTCTCAAAA+GGG 0.564962 4_2:-49704673 Msa0604250:CDS
GTGAAAGTATCCCATATACT+TGG 0.569026 4_2:+49705459 None:intergenic
GCAAATGGTGAAATGGCACC+AGG 0.576425 4_2:-49704814 Msa0604250:CDS
CCATCTTGACTGTAAGTCCA+TGG 0.578921 4_2:+49704841 None:intergenic
TTGCCACCAATTCATCAATG+AGG 0.580311 4_2:+49705127 None:intergenic
TGGTTGAATCCAAATTGCAC+CGG 0.581033 4_2:-49704778 Msa0604250:CDS
AAAGATATCAAAAGCTCTCA+GGG 0.581366 4_2:-49703136 Msa0604250:CDS
GTATCACTCACATGATGAGT+GGG 0.583322 4_2:+49705025 None:intergenic
AATCATGAAGGATATGAACT+TGG 0.589040 4_2:-49705352 Msa0604250:CDS
GGAAGATGTTGGAATCATGA+AGG 0.590169 4_2:-49705364 Msa0604250:CDS
AACCAAGAAGAGCATCGACC+TGG 0.592148 4_2:+49704796 None:intergenic
GGATAACTTTGAATGAGCCA+TGG 0.594373 4_2:-49704858 Msa0604250:CDS
TGATCCATCGTTATCACTTG+AGG 0.594621 4_2:-49703440 Msa0604250:CDS
AGTGGAGGAATTAATCAAGA+AGG 0.596031 4_2:-49705168 Msa0604250:CDS
GAAGGTGCAGCAAGTGAAGG+TGG 0.597164 4_2:-49705489 Msa0604250:CDS
CATGGACTTACAGTCAAGAT+GGG 0.597238 4_2:-49704840 Msa0604250:CDS
TATGCAGATGGAAAACTAAG+TGG 0.598010 4_2:-49705186 Msa0604250:intron
TATGAAGGTGCAGCAAGTGA+AGG 0.604375 4_2:-49705492 Msa0604250:CDS
AATAGAAAAGACTTCCCAGA+AGG 0.610702 4_2:-49705549 Msa0604250:CDS
TGTATCACTCACATGATGAG+TGG 0.613126 4_2:+49705024 None:intergenic
TTCCGTTCCAGAATCACCGC+CGG 0.613932 4_2:+49704759 None:intergenic
CACCGGCGGTGATTCTGGAA+CGG 0.616221 4_2:-49704761 Msa0604250:CDS
GTGATTGCAGTTGAACGTGA+TGG 0.617225 4_2:-49703836 Msa0604250:intron
TGGTAACCTTGTTCTTACCA+AGG 0.618332 4_2:+49704100 None:intergenic
ATGTAAAGGGATACTTTGCA+TGG 0.626161 4_2:-49703241 Msa0604250:CDS
TTGAATCCAAATTGCACCGG+CGG 0.626765 4_2:-49704775 Msa0604250:CDS
GGTTTGCCATTGCTCAACTG+AGG 0.628802 4_2:+49703987 None:intergenic
GATGCACCTCAGTTGAGCAA+TGG 0.634368 4_2:-49703993 Msa0604250:CDS
GCAGATGGAAAACTAAGTGG+AGG 0.645764 4_2:-49705183 Msa0604250:intron
GAGCAATCAAGGCTAGTGAG+TGG 0.679451 4_2:-49704065 Msa0604250:CDS
GATTCCTCAAGTGATAACGA+TGG 0.682607 4_2:+49703436 None:intergenic
TGAGAGCCTTGGTAAGAACA+AGG 0.686788 4_2:-49704106 Msa0604250:CDS
TGGAAACAATGGAGACGTTG+CGG 0.721837 4_2:-49705409 Msa0604250:CDS
ACTCTGGAAGATGAATATGG+CGG 0.785553 4_2:-49705057 Msa0604250:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATCATATATATATATATAG+GGG + chr4_2:49704530-49704549 None:intergenic 10.0%
!! GTTATCATATATATATATAT+AGG + chr4_2:49704532-49704551 None:intergenic 10.0%
!!! CTCTTTTTTTATTTAATTAT+AGG - chr4_2:49704557-49704576 Msa0604250:intron 10.0%
!! TTTATTTAATTATAGGTTTA+TGG - chr4_2:49704564-49704583 Msa0604250:intron 10.0%
!! TCAAACAAAAAATAATTAAA+TGG + chr4_2:49705726-49705745 None:intergenic 10.0%
!!! ATTTTTTGTTTGAATATAAA+AGG - chr4_2:49705733-49705752 Msa0604250:five_prime_UTR 10.0%
!! AGATAAACATATAATTGATA+GGG + chr4_2:49703472-49703491 None:intergenic 15.0%
!! TAACTTAGTTTAAATATTTG+TGG - chr4_2:49703744-49703763 Msa0604250:intron 15.0%
!! AAGGAGATATATATATATAT+GGG - chr4_2:49704217-49704236 Msa0604250:intron 15.0%
!!! AAATTGGTAATTAATGAATT+TGG - chr4_2:49704992-49705011 Msa0604250:intron 15.0%
!! TTTCAGTAATATAAATCAAA+GGG + chr4_2:49705110-49705129 None:intergenic 15.0%
!!! TTTTCAGTAATATAAATCAA+AGG + chr4_2:49705111-49705130 None:intergenic 15.0%
!! TTGATTTATATTACTGAAAA+TGG - chr4_2:49705111-49705130 Msa0604250:CDS 15.0%
!!! TTTCTTTTAATGTAAATACA+GGG - chr4_2:49705453-49705472 Msa0604250:CDS 15.0%
!!! AATAATTGTTATTACAACTA+TGG + chr4_2:49705703-49705722 None:intergenic 15.0%
!! TATTTACATACAATTTCAAA+TGG + chr4_2:49705766-49705785 None:intergenic 15.0%
!! ATATAATCTCTATATGTAAT+TGG - chr4_2:49705783-49705802 Msa0604250:five_prime_UTR 15.0%
!! GAGATAAACATATAATTGAT+AGG + chr4_2:49703473-49703492 None:intergenic 20.0%
!!! ATTTGTAACACTTTTTCATT+GGG - chr4_2:49703635-49703654 Msa0604250:CDS 20.0%
!! AGATAACAAACTTGATATAA+AGG - chr4_2:49704121-49704140 Msa0604250:CDS 20.0%
!! CAAGGAGATATATATATATA+TGG - chr4_2:49704216-49704235 Msa0604250:intron 20.0%
!! AGGAGATATATATATATATG+GGG - chr4_2:49704218-49704237 Msa0604250:intron 20.0%
!! ATCAATAGATCATATAAAGT+AGG + chr4_2:49704816-49704835 None:intergenic 20.0%
!!! TAATTGGTTTTTGTCTAAAT+TGG - chr4_2:49704976-49704995 Msa0604250:intron 20.0%
!!! AATTTGGTTATTTTATTTCC+AGG - chr4_2:49705008-49705027 Msa0604250:intron 20.0%
!! TCATTTATTCTATCTTAATG+AGG - chr4_2:49705349-49705368 Msa0604250:CDS 20.0%
!!! CTTTCTTTTAATGTAAATAC+AGG - chr4_2:49705452-49705471 Msa0604250:CDS 20.0%
! AGAATGAATGAATGAATGAA+TGG + chr4_2:49702949-49702968 None:intergenic 25.0%
! AAGACAAGAAGAGAATAAAT+AGG + chr4_2:49703080-49703099 None:intergenic 25.0%
! TTCATCATTACTTTAACAGA+AGG - chr4_2:49703111-49703130 Msa0604250:CDS 25.0%
!! CATACAGATTTTCTATATCT+TGG - chr4_2:49703400-49703419 Msa0604250:CDS 25.0%
! ACTTCATTAATTTATGCAGA+TGG - chr4_2:49703528-49703547 Msa0604250:intron 25.0%
!!! CAATGAAAAAGTGTTACAAA+TGG + chr4_2:49703636-49703655 None:intergenic 25.0%
!!! CATTTGTAACACTTTTTCAT+TGG - chr4_2:49703634-49703653 Msa0604250:CDS 25.0%
!! TGATAATGTGCAACTAAATA+AGG + chr4_2:49703993-49704012 None:intergenic 25.0%
! TAATCATCCAAATTAACACA+AGG - chr4_2:49704198-49704217 Msa0604250:intron 25.0%
! TATATCTCCTTGTGTTAATT+TGG + chr4_2:49704208-49704227 None:intergenic 25.0%
! GCAAGTAAATAACTAAAAAG+TGG + chr4_2:49704315-49704334 None:intergenic 25.0%
! GCAAGTAAATAACTAAAAAG+TGG + chr4_2:49704315-49704334 None:intergenic 25.0%
!!! AGTGGTTCATTTGATTTTAT+TGG - chr4_2:49704679-49704698 Msa0604250:CDS 25.0%
!!! AACTTTTTCATAATGTGTGT+AGG - chr4_2:49705248-49705267 Msa0604250:intron 25.0%
! GATGATGATGACAAAAACAA+AGG + chr4_2:49703016-49703035 None:intergenic 30.0%
TATCCTCAAAAAATCACTGA+TGG - chr4_2:49703297-49703316 Msa0604250:intron 30.0%
!!! TTTCCATCAGTGATTTTTTG+AGG + chr4_2:49703303-49703322 None:intergenic 30.0%
AAAATCACTGATGGAAACAA+TGG - chr4_2:49703306-49703325 Msa0604250:intron 30.0%
AATCATGAAGGATATGAACT+TGG - chr4_2:49703374-49703393 Msa0604250:CDS 30.0%
TCAATTGACTAGACATACTT+GGG + chr4_2:49703437-49703456 None:intergenic 30.0%
TTCAATTGACTAGACATACT+TGG + chr4_2:49703438-49703457 None:intergenic 30.0%
GAACTTTGCTTCAAAACATT+TGG - chr4_2:49703828-49703847 Msa0604250:CDS 30.0%
TTGGAGATAGAGTAAAACAT+TGG - chr4_2:49703847-49703866 Msa0604250:intron 30.0%
! AAAGTATCAAGTCTCTCAAA+AGG - chr4_2:49704052-49704071 Msa0604250:CDS 30.0%
AAGTATCAAGTCTCTCAAAA+GGG - chr4_2:49704053-49704072 Msa0604250:CDS 30.0%
!!! ACACCCACTATTTTTTATGT+GGG - chr4_2:49704252-49704271 Msa0604250:intron 30.0%
ACTCCCACATAAAAAATAGT+GGG + chr4_2:49704258-49704277 None:intergenic 30.0%
ACCTTCTAACAATAACAAGT+GGG + chr4_2:49704406-49704425 None:intergenic 30.0%
TTATAGCAACATACACCTTA+GGG - chr4_2:49704429-49704448 Msa0604250:intron 30.0%
! GATTGCTCTTTTGTAAACTT+TGG + chr4_2:49704649-49704668 None:intergenic 30.0%
AGTTTACAAAAGAGCAATCA+AGG - chr4_2:49704650-49704669 Msa0604250:intron 30.0%
TCATATAAAGTAGGCCAAAA+GGG + chr4_2:49704807-49704826 None:intergenic 30.0%
ATCATATAAAGTAGGCCAAA+AGG + chr4_2:49704808-49704827 None:intergenic 30.0%
AACACTTACATTTAGACCAA+TGG + chr4_2:49704921-49704940 None:intergenic 30.0%
TGAGATATTGCAACATTTCA+TGG + chr4_2:49704951-49704970 None:intergenic 30.0%
GCAATATCTCATCTGTTAAT+TGG - chr4_2:49704960-49704979 Msa0604250:intron 30.0%
GTCTATTTCACTTTCTTTCA+TGG - chr4_2:49705410-49705429 Msa0604250:CDS 30.0%
! TTTTAATGTAAATACAGGGC+TGG - chr4_2:49705457-49705476 Msa0604250:CDS 30.0%
! CTTTGTTGGACAATTTTGAA+TGG - chr4_2:49705509-49705528 Msa0604250:CDS 30.0%
ACCTTCGTAGATTACAAAAA+TGG - chr4_2:49705562-49705581 Msa0604250:CDS 30.0%
!! ACCATTTTTGTAATCTACGA+AGG + chr4_2:49705566-49705585 None:intergenic 30.0%
AAAAGATATCAAAAGCTCTC+AGG - chr4_2:49705589-49705608 Msa0604250:CDS 30.0%
AAAGATATCAAAAGCTCTCA+GGG - chr4_2:49705590-49705609 Msa0604250:CDS 30.0%
AATGTTGTAGCACTGTTAAA+TGG + chr4_2:49705668-49705687 None:intergenic 30.0%
TGATGACAAAAACAAAGGTG+TGG + chr4_2:49703011-49703030 None:intergenic 35.0%
AACACTGAATCACTGCAAAT+AGG - chr4_2:49703147-49703166 Msa0604250:CDS 35.0%
AATAGAAAAGACTTCCCAGA+AGG - chr4_2:49703177-49703196 Msa0604250:CDS 35.0%
! TTCCCAGAAGGCTTTATATT+TGG - chr4_2:49703189-49703208 Msa0604250:CDS 35.0%
TGCCAAATATAAAGCCTTCT+GGG + chr4_2:49703194-49703213 None:intergenic 35.0%
TCTTCTGCATATCAGTATGA+AGG - chr4_2:49703219-49703238 Msa0604250:CDS 35.0%
!! GTGAAAGTATCCCATATACT+TGG + chr4_2:49703270-49703289 None:intergenic 35.0%
TATGCAGATGGAAAACTAAG+TGG - chr4_2:49703540-49703559 Msa0604250:intron 35.0%
AGTGGAGGAATTAATCAAGA+AGG - chr4_2:49703558-49703577 Msa0604250:intron 35.0%
CAACAACCTCATTGATGAAT+TGG - chr4_2:49703593-49703612 Msa0604250:intron 35.0%
! ATTGATGAATTGGTGGCAAA+TGG - chr4_2:49703603-49703622 Msa0604250:intron 35.0%
TATTCATCTTCCAGAGTTTG+AGG + chr4_2:49703666-49703685 None:intergenic 35.0%
! CAAACTCTGGAAGATGAATA+TGG - chr4_2:49703666-49703685 Msa0604250:CDS 35.0%
AAACTGCAGAAAAGATTTCC+AGG - chr4_2:49703797-49703816 Msa0604250:intron 35.0%
!! TCAAGTTTGTTATCTGAGAG+TGG + chr4_2:49704116-49704135 None:intergenic 35.0%
!!! CACACCCACTATTTTTTATG+TGG - chr4_2:49704251-49704270 Msa0604250:intron 35.0%
CACTCCCACATAAAAAATAG+TGG + chr4_2:49704259-49704278 None:intergenic 35.0%
! TTTTAGCAAAGCTACACCTT+AGG - chr4_2:49704279-49704298 Msa0604250:intron 35.0%
TTTAGCAAAGCTACACCTTA+GGG - chr4_2:49704280-49704299 Msa0604250:intron 35.0%
AAGTGGTTAATTACACCCTA+AGG + chr4_2:49704298-49704317 None:intergenic 35.0%
ACTCCCACATAAAAACTAGT+GGG - chr4_2:49704341-49704360 Msa0604250:intron 35.0%
!!! ACACCCACTAGTTTTTATGT+GGG + chr4_2:49704347-49704366 None:intergenic 35.0%
TGTGTTCTAGCAAATCCTAT+AGG + chr4_2:49704383-49704402 None:intergenic 35.0%
!! ACCCACTTGTTATTGTTAGA+AGG - chr4_2:49704402-49704421 Msa0604250:intron 35.0%
CACCTTCTAACAATAACAAG+TGG + chr4_2:49704407-49704426 None:intergenic 35.0%
GTTATAGCAACATACACCTT+AGG - chr4_2:49704428-49704447 Msa0604250:intron 35.0%
AAGTGGTTAAATACACCCTA+AGG + chr4_2:49704447-49704466 None:intergenic 35.0%
ACTCCCACATAAAAACTAGT+GGG - chr4_2:49704341-49704360 Msa0604250:intron 35.0%
!!! ACACCCACTAGTTTTTATGT+GGG + chr4_2:49704347-49704366 None:intergenic 35.0%
TTATAGGTTTATGGATCCCT+TGG - chr4_2:49704573-49704592 Msa0604250:intron 35.0%
!! GGTTTTTGGATAATCTCCAT+TGG + chr4_2:49704599-49704618 None:intergenic 35.0%
AACGAGAAAGAGAATCTGTA+AGG + chr4_2:49704771-49704790 None:intergenic 35.0%
! TTTTCATGTAAGCACCCTTT+TGG - chr4_2:49704790-49704809 Msa0604250:CDS 35.0%
ATTTCCAGGTTGCTTCAAAT+TGG - chr4_2:49705022-49705041 Msa0604250:intron 35.0%
ATCACTTGAGGAATCACTTA+TGG - chr4_2:49705298-49705317 Msa0604250:intron 35.0%
ATGTAAAGGGATACTTTGCA+TGG - chr4_2:49705485-49705504 Msa0604250:CDS 35.0%
ATACTTTGCATGGTCTTTGT+TGG - chr4_2:49705495-49705514 Msa0604250:CDS 35.0%
! GACAATTTTGAATGGCACAA+GGG - chr4_2:49705517-49705536 Msa0604250:CDS 35.0%
AAGGGTTATACTGTCAGATT+TGG - chr4_2:49705535-49705554 Msa0604250:CDS 35.0%
GTGCCAAATATAAAGCCTTC+TGG + chr4_2:49703195-49703214 None:intergenic 40.0%
TGGAAGAGGACCAAGTATAT+GGG - chr4_2:49703257-49703276 Msa0604250:intron 40.0%
TGACTCGTATCATCGCTATA+AGG - chr4_2:49703341-49703360 Msa0604250:intron 40.0%
CATCGCTATAAGGAAGATGT+TGG - chr4_2:49703351-49703370 Msa0604250:intron 40.0%
GGAAGATGTTGGAATCATGA+AGG - chr4_2:49703362-49703381 Msa0604250:intron 40.0%
CAACCTCATTGATGAATTGG+TGG - chr4_2:49703596-49703615 Msa0604250:intron 40.0%
TTGCCACCAATTCATCAATG+AGG + chr4_2:49703602-49703621 None:intergenic 40.0%
! ACTCTGGAAGATGAATATGG+CGG - chr4_2:49703669-49703688 Msa0604250:CDS 40.0%
GTATCACTCACATGATGAGT+GGG + chr4_2:49703704-49703723 None:intergenic 40.0%
TGTATCACTCACATGATGAG+TGG + chr4_2:49703705-49703724 None:intergenic 40.0%
GGATAACTTTGAATGAGCCA+TGG - chr4_2:49703868-49703887 Msa0604250:intron 40.0%
CATGGACTTACAGTCAAGAT+GGG - chr4_2:49703886-49703905 Msa0604250:intron 40.0%
AGTCAAGATGGGTATGCAAA+TGG - chr4_2:49703897-49703916 Msa0604250:intron 40.0%
TGGGTATGCAAATGGTGAAA+TGG - chr4_2:49703905-49703924 Msa0604250:intron 40.0%
TGGTTGAATCCAAATTGCAC+CGG - chr4_2:49703948-49703967 Msa0604250:intron 40.0%
GGGTGTGATAGGTATAACAT+TGG - chr4_2:49704073-49704092 Msa0604250:CDS 40.0%
AGAGCTACAGACTTCATGTT+TGG - chr4_2:49704152-49704171 Msa0604250:intron 40.0%
CTACAGACTTCATGTTTGGA+TGG - chr4_2:49704156-49704175 Msa0604250:intron 40.0%
CACTCCCACATAAAAACTAG+TGG - chr4_2:49704340-49704359 Msa0604250:intron 40.0%
!!! CACACCCACTAGTTTTTATG+TGG + chr4_2:49704348-49704367 None:intergenic 40.0%
GTGTTCTAGCAAATGCCTAT+AGG - chr4_2:49704365-49704384 Msa0604250:intron 40.0%
CACTCCCACATAAAAACTAG+TGG - chr4_2:49704340-49704359 Msa0604250:intron 40.0%
!!! CACACCCACTAGTTTTTATG+TGG + chr4_2:49704348-49704367 None:intergenic 40.0%
TGGTAACCTTGTTCTTACCA+AGG + chr4_2:49704629-49704648 None:intergenic 40.0%
AGAGAATCTGTAAGGTAGCT+AGG + chr4_2:49704763-49704782 None:intergenic 40.0%
! GAACGTGATGGAAAAACCAT+TGG - chr4_2:49704902-49704921 Msa0604250:CDS 40.0%
! ACAACCAATTTGAAGCAACC+TGG + chr4_2:49705029-49705048 None:intergenic 40.0%
AGAAAGTCTCGAATTGCTCT+TGG + chr4_2:49705060-49705079 None:intergenic 40.0%
TGATCCATCGTTATCACTTG+AGG - chr4_2:49705286-49705305 Msa0604250:intron 40.0%
! GATTCCTCAAGTGATAACGA+TGG + chr4_2:49705293-49705312 None:intergenic 40.0%
! AGGGCTGGTGTAAATGTAAA+GGG - chr4_2:49705472-49705491 Msa0604250:CDS 40.0%
! GGACAATTTTGAATGGCACA+AGG - chr4_2:49705516-49705535 Msa0604250:CDS 40.0%
! TATGAAGGTGCAGCAAGTGA+AGG - chr4_2:49703234-49703253 Msa0604250:CDS 45.0%
GTGGAAGAGGACCAAGTATA+TGG - chr4_2:49703256-49703275 Msa0604250:intron 45.0%
TGGAAACAATGGAGACGTTG+CGG - chr4_2:49703317-49703336 Msa0604250:intron 45.0%
GCAGATGGAAAACTAAGTGG+AGG - chr4_2:49703543-49703562 Msa0604250:intron 45.0%
TTGGGATCTTCCTCAAACTC+TGG - chr4_2:49703653-49703672 Msa0604250:CDS 45.0%
AGCAAAGTTCTGCATAGTCC+TGG + chr4_2:49703818-49703837 None:intergenic 45.0%
CCATCTTGACTGTAAGTCCA+TGG + chr4_2:49703888-49703907 None:intergenic 45.0%
CCATGGACTTACAGTCAAGA+TGG - chr4_2:49703885-49703904 Msa0604250:intron 45.0%
TTGAATCCAAATTGCACCGG+CGG - chr4_2:49703951-49703970 Msa0604250:intron 45.0%
! GTCTCTCAAAAGGGTGTGAT+AGG - chr4_2:49704062-49704081 Msa0604250:CDS 45.0%
TTTATGGATCCCTTGGCCAA+TGG - chr4_2:49704580-49704599 Msa0604250:intron 45.0%
GGATAATCTCCATTGGCCAA+GGG + chr4_2:49704592-49704611 None:intergenic 45.0%
TGGATAATCTCCATTGGCCA+AGG + chr4_2:49704593-49704612 None:intergenic 45.0%
TCCAAAAACCATGAGAGCCT+TGG - chr4_2:49704609-49704628 Msa0604250:intron 45.0%
! ACCAAGGCTCTCATGGTTTT+TGG + chr4_2:49704613-49704632 None:intergenic 45.0%
TGTTCTTACCAAGGCTCTCA+TGG + chr4_2:49704620-49704639 None:intergenic 45.0%
TGAGAGCCTTGGTAAGAACA+AGG - chr4_2:49704620-49704639 Msa0604250:intron 45.0%
GTGATTGCAGTTGAACGTGA+TGG - chr4_2:49704890-49704909 Msa0604250:CDS 45.0%
! CAGGGCTGGTGTAAATGTAA+AGG - chr4_2:49705471-49705490 Msa0604250:CDS 45.0%
ATCAAAAGCTCTCAGGGTTG+TGG - chr4_2:49705596-49705615 Msa0604250:CDS 45.0%
!! TAATTAAAAATAATGAAATA+TGG + chr4_2:49703504-49703523 None:intergenic 5.0%
!! TTATCATATATATATATATA+GGG + chr4_2:49704531-49704550 None:intergenic 5.0%
!! CTATTAAAAATTTAAATTAA+TGG - chr4_2:49704847-49704866 Msa0604250:CDS 5.0%
GCAAATGGTGAAATGGCACC+AGG - chr4_2:49703912-49703931 Msa0604250:intron 50.0%
!! AACCAAGAAGAGCATCGACC+TGG + chr4_2:49703933-49703952 None:intergenic 50.0%
GAGCAATCAAGGCTAGTGAG+TGG - chr4_2:49704661-49704680 Msa0604250:intron 50.0%
GATGCACCTCAGTTGAGCAA+TGG - chr4_2:49704733-49704752 Msa0604250:CDS 50.0%
GGTTTGCCATTGCTCAACTG+AGG + chr4_2:49704742-49704761 None:intergenic 50.0%
! GAAGGTGCAGCAAGTGAAGG+TGG - chr4_2:49703237-49703256 Msa0604250:CDS 55.0%
GCAGCAAGTGAAGGTGGAAG+AGG - chr4_2:49703243-49703262 Msa0604250:CDS 55.0%
CACCAGGTCGATGCTCTTCT+TGG - chr4_2:49703928-49703947 Msa0604250:intron 55.0%
GAATCACCGCCGGTGCAATT+TGG + chr4_2:49703960-49703979 None:intergenic 55.0%
! AATTGCACCGGCGGTGATTC+TGG - chr4_2:49703960-49703979 Msa0604250:CDS 55.0%
TTCCGTTCCAGAATCACCGC+CGG + chr4_2:49703970-49703989 None:intergenic 55.0%
! CACCGGCGGTGATTCTGGAA+CGG - chr4_2:49703965-49703984 Msa0604250:CDS 60.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 49702941 49705807 49702941 ID=Msa0604250;Name=Msa0604250
chr4_2 mRNA 49702941 49705807 49702941 ID=Msa0604250-mRNA-1;Parent=Msa0604250;Name=Msa0604250-mRNA-1;_AED=0.12;_eAED=0.12;_QI=125|0.85|0.75|1|1|1|8|135|483
chr4_2 exon 49702941 49703274 49702941 ID=Msa0604250-mRNA-1:exon:11547;Parent=Msa0604250-mRNA-1
chr4_2 exon 49703370 49703478 49703370 ID=Msa0604250-mRNA-1:exon:11546;Parent=Msa0604250-mRNA-1
chr4_2 exon 49703616 49703718 49703616 ID=Msa0604250-mRNA-1:exon:11545;Parent=Msa0604250-mRNA-1
chr4_2 exon 49703817 49703848 49703817 ID=Msa0604250-mRNA-1:exon:11544;Parent=Msa0604250-mRNA-1
chr4_2 exon 49703952 49704143 49703952 ID=Msa0604250-mRNA-1:exon:11543;Parent=Msa0604250-mRNA-1
chr4_2 exon 49704665 49704942 49704665 ID=Msa0604250-mRNA-1:exon:11542;Parent=Msa0604250-mRNA-1
chr4_2 exon 49705036 49705201 49705036 ID=Msa0604250-mRNA-1:exon:11541;Parent=Msa0604250-mRNA-1
chr4_2 exon 49705310 49705807 49705310 ID=Msa0604250-mRNA-1:exon:11540;Parent=Msa0604250-mRNA-1
chr4_2 five_prime_UTR 49705683 49705807 49705683 ID=Msa0604250-mRNA-1:five_prime_utr;Parent=Msa0604250-mRNA-1
chr4_2 CDS 49705310 49705682 49705310 ID=Msa0604250-mRNA-1:cds;Parent=Msa0604250-mRNA-1
chr4_2 CDS 49705036 49705201 49705036 ID=Msa0604250-mRNA-1:cds;Parent=Msa0604250-mRNA-1
chr4_2 CDS 49704665 49704942 49704665 ID=Msa0604250-mRNA-1:cds;Parent=Msa0604250-mRNA-1
chr4_2 CDS 49703952 49704143 49703952 ID=Msa0604250-mRNA-1:cds;Parent=Msa0604250-mRNA-1
chr4_2 CDS 49703817 49703848 49703817 ID=Msa0604250-mRNA-1:cds;Parent=Msa0604250-mRNA-1
chr4_2 CDS 49703616 49703718 49703616 ID=Msa0604250-mRNA-1:cds;Parent=Msa0604250-mRNA-1
chr4_2 CDS 49703370 49703478 49703370 ID=Msa0604250-mRNA-1:cds;Parent=Msa0604250-mRNA-1
chr4_2 CDS 49703076 49703274 49703076 ID=Msa0604250-mRNA-1:cds;Parent=Msa0604250-mRNA-1
chr4_2 three_prime_UTR 49702941 49703075 49702941 ID=Msa0604250-mRNA-1:three_prime_utr;Parent=Msa0604250-mRNA-1
Gene Sequence

>Msa0604250

ATGAGATATTTCCTATTTATTCTCTTCTTGTCTTTAGTTTCAACATTCATCATTACTTTAACAGAAGGTCAAGAAATAATTAACACTGAATCACTGCAAATAGGTTCATTGAATAGAAAAGACTTCCCAGAAGGCTTTATATTTGGCACAGCATCTTCTGCATATCAGTATGAAGGTGCAGCAAGTGAAGGTGGAAGAGGACCAAGTATATGGGATACTTTCACTCATAGATATCCTCAAAAAATCACTGATGGAAACAATGGAGACGTTGCGGTTGACTCGTATCATCGCTATAAGGAAGATGTTGGAATCATGAAGGATATGAACTTGGATGCATACAGATTTTCTATATCTTGGTCTAGAATACTCCCAAATGGAAAACTAAGTGGAGGAATTAATCAAGAAGGAATCGACTATTACAACAACCTCATTGATGAATTGGTGGCAAATGGATTACAACCATTTGTAACACTTTTTCATTGGGATCTTCCTCAAACTCTGGAAGATGAATATGGCGGTTTCTTAAGCCCACTCATCATAAAAGATTTCCAGGACTATGCAGAACTTTGCTTCAAAACATTTGGAGATAGAGTAAAACATTGGATAACTTTGAATGAGCCATGGACTTACAGTCAAGATGGGTATGCAAATGGTGAAATGGCACCAGGTCGATGCTCTTCTTGGTTGAATCCAAATTGCACCGGCGGTGATTCTGGAACGGAACCTTATTTAGTTGCACATTATCAACTTCTAGCTCATGCAACAGTTGTTAATTTATACAAGACAAAGTATCAAGTCTCTCAAAAGGGTGTGATAGATTATCCAAAAACCATGAGAGCCTTGGTAAGAACAAGGTTACCAAAGTTTACAAAAGAGCAATCAAGGCTAGTGAGTGGTTCATTTGATTTTATTGGCATAAATTACTACTCTTCATGCTATGCTTCTGATGCACCTCAGTTGAGCAATGGCAAACCTAGCTACCTTACAGATTCTCTTTCTCGTTTTTCATTTGAACGTGATGGAAAAACCATTGGTCTAAATGTTGCTTCAAATTGGTTGTATGTTTATCCAAGAGCAATTCGAGACTTTCTGATACAAGTTAAAGAAAAATACAACAACCCTTTGATTTATATTACTGAAAATGGTATTAATGAGTACGATGATCCATCGTTATCACTTGAGGAATCACTTATGGATACGTATAGAGTTGATTATCATTATCGTCATTTATTCTATCTTAATGAGGCAATCAAGGCTGGTGTAAATGTAAAGGGATACTTTGCATGGTCTTTGTTGGACAATTTTGAATGGCACAAGGGTTATACTGTCAGATTTGGAATGACCTTCGTAGATTACAAAAATGGTTTGAAAAGATATCAAAAGCTCTCAGGGTTGTGGTTCAAGAATTTTCTCACATCAGACAACAAAAAAGTTTACATAGATTCCATTTAA
Protein sequence

>Msa0604250

MRYFLFILFLSLVSTFIITLTEGQEIINTESLQIGSLNRKDFPEGFIFGTASSAYQYEGAASEGGRGPSIWDTFTHRYPQKITDGNNGDVAVDSYHRYKEDVGIMKDMNLDAYRFSISWSRILPNGKLSGGINQEGIDYYNNLIDELVANGLQPFVTLFHWDLPQTLEDEYGGFLSPLIIKDFQDYAELCFKTFGDRVKHWITLNEPWTYSQDGYANGEMAPGRCSSWLNPNCTGGDSGTEPYLVAHYQLLAHATVVNLYKTKYQVSQKGVIDYPKTMRALVRTRLPKFTKEQSRLVSGSFDFIGINYYSSCYASDAPQLSNGKPSYLTDSLSRFSFERDGKTIGLNVASNWLYVYPRAIRDFLIQVKEKYNNPLIYITENGINEYDDPSLSLEESLMDTYRVDYHYRHLFYLNEAIKAGVNVKGYFAWSLLDNFEWHKGYTVRFGMTFVDYKNGLKRYQKLSGLWFKNFLTSDNKKVYIDSI*