AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0650760

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0650760 MtrunA17_Chr4g0043001 94.343 548 31 0 1 548 1 548 0.0 1083
Msa0650760 MtrunA17_Chr4g0043021 83.789 549 87 2 1 548 1 548 0.0 936
Msa0650760 MtrunA17_Chr4g0022251 36.062 452 228 16 85 518 173 581 1.43e-68 233
Msa0650760 MtrunA17_Chr1g0173511 33.118 465 236 16 85 518 36 456 3.87e-64 217
Msa0650760 MtrunA17_Chr8g0364481 32.210 534 281 21 25 518 46 538 7.85e-64 219
Msa0650760 MtrunA17_Chr5g0419621 34.279 458 236 18 83 518 131 545 1.40e-61 213
Msa0650760 MtrunA17_Chr4g0057551 30.755 556 293 21 20 529 55 564 8.90e-58 203
Msa0650760 MtrunA17_Chr3g0116051 29.541 545 294 20 20 518 49 549 7.63e-57 201
Msa0650760 MtrunA17_Chr3g0116091 27.273 407 214 15 128 518 5 345 4.42e-36 139
Msa0650760 MtrunA17_Chr7g0266151 27.456 397 220 11 144 537 47 378 1.52e-31 126
Msa0650760 MtrunA17_Chr7g0266131 30.649 385 202 17 118 496 30 355 2.94e-31 126
Msa0650760 MtrunA17_Chr5g0443881 28.818 406 211 16 144 546 49 379 1.46e-28 118
Msa0650760 MtrunA17_Chr1g0178831 28.045 353 190 12 140 487 42 335 2.37e-26 111
Msa0650760 MtrunA17_Chr3g0098721 27.586 406 216 15 144 546 43 373 3.15e-26 111
Msa0650760 MtrunA17_Chr3g0121411 28.000 425 216 16 139 519 66 444 4.39e-24 105
Msa0650760 MtrunA17_Chr3g0121431 27.934 426 215 17 139 519 121 499 1.60e-23 104
Msa0650760 MtrunA17_Chr7g0236041 25.386 453 238 17 139 548 65 460 4.16e-21 97.1
Msa0650760 MtrunA17_Chr4g0019091 25.243 412 246 12 143 546 54 411 6.92e-21 95.9
Msa0650760 MtrunA17_Chr3g0116071 32.558 172 91 7 192 343 48 214 7.73e-20 89.4
Msa0650760 MtrunA17_Chr6g0453731 26.266 316 163 9 212 496 146 422 3.25e-19 91.3
Msa0650760 MtrunA17_Chr4g0064541 24.408 422 236 16 143 546 54 410 1.45e-18 88.6
Msa0650760 MtrunA17_Chr1g0211041 24.943 441 250 16 125 546 35 413 6.98e-18 86.7
Msa0650760 MtrunA17_Chr4g0019071 25.564 266 164 6 287 546 51 288 3.11e-13 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0650760 AT1G13900.1 63.553 546 188 4 6 548 8 545 0.0 745
Msa0650760 AT2G03450.1 63.570 549 185 6 5 548 7 545 0.0 726
Msa0650760 AT1G13750.1 33.462 520 256 21 20 495 42 515 1.92e-66 227
Msa0650760 AT5G50400.1 32.353 510 282 18 44 529 78 548 1.56e-63 219
Msa0650760 AT5G50400.2 32.985 479 263 17 70 529 37 476 6.89e-63 215
Msa0650760 AT4G24890.1 29.616 547 306 21 11 518 35 541 4.48e-57 201
Msa0650760 AT3G52820.1 30.548 383 195 14 140 518 43 358 2.69e-32 129
Msa0650760 AT3G52780.1 30.556 396 212 13 144 537 44 378 1.48e-31 127
Msa0650760 AT3G52780.2 31.412 347 184 12 144 488 44 338 2.57e-31 125
Msa0650760 AT3G52810.1 30.618 356 179 13 144 492 51 345 3.68e-28 117
Msa0650760 AT3G20500.1 26.500 400 226 12 140 537 43 376 8.91e-26 110
Msa0650760 AT4G13700.1 29.716 387 213 14 139 496 60 416 2.41e-25 109
Msa0650760 AT3G07130.1 30.409 342 160 13 212 524 140 432 1.52e-21 98.6
Msa0650760 AT4G13700.2 28.726 369 207 13 139 481 60 398 6.06e-21 95.9
Msa0650760 AT2G27190.1 27.841 352 184 15 212 546 118 416 1.03e-19 92.4
Msa0650760 AT2G32770.3 26.817 399 214 16 144 496 69 435 9.02e-19 90.1
Msa0650760 AT3G07130.2 29.582 311 151 11 212 494 140 410 1.78e-18 89.4
Msa0650760 AT2G32770.2 28.803 309 174 11 212 496 68 354 3.33e-18 87.8
Msa0650760 AT4G36350.1 28.116 345 191 13 212 546 114 411 1.00e-17 86.3
Msa0650760 AT1G56360.1 26.979 341 200 10 212 546 114 411 1.61e-17 85.9
Msa0650760 AT5G34850.1 24.819 415 243 16 143 546 53 409 1.06e-16 83.2
Msa0650760 AT2G18130.1 25.882 255 145 7 303 546 171 392 8.09e-15 77.4
Msa0650760 AT2G32770.1 26.087 391 198 18 144 496 69 406 8.44e-11 65.1

Find 199 sgRNAs with CRISPR-Local

Find 293 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTGGTGCTTGGGGCCTTTA+TGG 0.124406 4_3:+58007956 Msa0650760:three_prime_UTR
GGAAACCTTCTCGCCATTTC+TGG 0.134192 4_3:+58005316 Msa0650760:CDS
GCATGACACAATGGAAATTT+TGG 0.186747 4_3:+58007824 Msa0650760:three_prime_UTR
TCTTTATTACTCATTTGATA+TGG 0.193459 4_3:+58007271 Msa0650760:CDS
TACAGCCATGGAAACCTAAT+TGG 0.237477 4_3:+58007135 Msa0650760:CDS
AAACCTAATTGGACTAATTA+TGG 0.245800 4_3:+58007146 Msa0650760:CDS
CAATGTGAACCTTGCCCTTT+GGG 0.254495 4_3:+58007514 Msa0650760:CDS
GCCTTTATGGGTTACATTCT+CGG 0.263370 4_3:+58007969 Msa0650760:three_prime_UTR
GGAGAGGTTTCGTTTGTTTC+GGG 0.266915 4_3:+58005337 Msa0650760:CDS
TGGAGAGGTTTCGTTTGTTT+CGG 0.270883 4_3:+58005336 Msa0650760:CDS
TAACTTCTTGAAGCATGATT+TGG 0.286113 4_3:+58007355 Msa0650760:CDS
CGCTTCCTCCCTGGACATAC+TGG 0.298280 4_3:-58007932 None:intergenic
GAGGAAGCGTACTGGTGCTT+GGG 0.309443 4_3:+58007946 Msa0650760:three_prime_UTR
TTTCCATAATTAGTCCAATT+AGG 0.311578 4_3:-58007149 None:intergenic
CTGGTGCTTGGGGCCTTTAT+GGG 0.316117 4_3:+58007957 Msa0650760:three_prime_UTR
AGAAATGGCGAGAAGGTTTC+CGG 0.335298 4_3:-58005314 None:intergenic
CCACGATACAAAGACTGTTT+TGG 0.338175 4_3:-58007719 None:intergenic
GGTAATGTTAATGTTGATAT+TGG 0.343693 4_3:+58007867 Msa0650760:three_prime_UTR
GCTTCTTCAATCAAACCTTT+AGG 0.347032 4_3:-58007900 None:intergenic
AATAACTACACTTGTGGAAA+TGG 0.347897 4_3:+58007572 Msa0650760:CDS
ATCAGGGTTGATTTCGGATT+GGG 0.356573 4_3:-58005257 None:intergenic
GCGGTGTACCCTATAGTTTA+AGG 0.356975 4_3:+58007189 Msa0650760:CDS
TGAGCACTTGGAACCTCTAT+TGG 0.358489 4_3:+58007484 Msa0650760:CDS
TCCACCTGGCAATCCGGTTC+CGG 0.362173 4_3:+58005172 Msa0650760:CDS
ACAATGTGAACCTTGCCCTT+TGG 0.362789 4_3:+58007513 Msa0650760:CDS
TAATAAAGATTTCTAGTTGC+TGG 0.363307 4_3:-58007257 None:intergenic
GGAGGAAGCGTACTGGTGCT+TGG 0.371645 4_3:+58007945 Msa0650760:three_prime_UTR
GTAGGTTCTGACGAATTTCC+AGG 0.373251 4_3:-58007221 None:intergenic
TGAAGATTATTGAAAGATAG+AGG 0.374624 4_3:-58004961 None:intergenic
TCCATTGATTGTTGTGTACA+TGG 0.378076 4_3:-58007429 None:intergenic
AATGGCGTGGGCCGGAGAGC+AGG 0.380124 4_3:+58007590 Msa0650760:CDS
CATGCTTCAAGAAGTTATAC+TGG 0.380553 4_3:-58007349 None:intergenic
ATAACGATCAGGGTTGATTT+CGG 0.381601 4_3:-58005263 None:intergenic
CTTTATTACTCATTTGATAT+GGG 0.382012 4_3:+58007272 Msa0650760:CDS
GTTGCTCCCAAGAAGAAAAT+TGG 0.386841 4_3:-58007327 None:intergenic
GAGGGAGAGTGTGCCGGAAC+CGG 0.388793 4_3:-58005185 None:intergenic
TGGGGAGAGAAAATGTCATA+TGG 0.392406 4_3:+58005459 Msa0650760:CDS
AGGAAGAAGATACTACTATA+AGG 0.392651 4_3:+58005609 Msa0650760:CDS
GTTTCGTTTGTTTCGGGTAA+AGG 0.396676 4_3:+58005343 Msa0650760:CDS
CATCTTGTGATTGGCATGGC+AGG 0.399832 4_3:+58007632 Msa0650760:CDS
CATGTTGAACCTTAAACTAT+AGG 0.406709 4_3:-58007198 None:intergenic
CCCACAACTCTATCAAAATC+CGG 0.407569 4_3:+58005025 Msa0650760:CDS
TTGAGGAAAGATCGAGTCAT+TGG 0.409792 4_3:-58007696 None:intergenic
TTCTCTCAACGCAACATCCT+TGG 0.412679 4_3:-58007453 None:intergenic
TTGTCATGGTCATAACGATC+AGG 0.414015 4_3:-58005274 None:intergenic
TATACCGTTCATCTTGTGAT+TGG 0.415416 4_3:+58007623 Msa0650760:CDS
AGAAAAGATGCTTGAGCACT+TGG 0.415912 4_3:+58007472 Msa0650760:CDS
TGGACTAATTATGGAAAAGA+TGG 0.416929 4_3:+58007155 Msa0650760:CDS
GCGAGAAGGTTTCCGGTTTG+TGG 0.419688 4_3:-58005307 None:intergenic
TGGATGATTTAGAGAGGAAG+AGG 0.425118 4_3:-58005153 None:intergenic
ACCGAGAATGTAACCCATAA+AGG 0.427471 4_3:-58007970 None:intergenic
AGTCTAGCTCTGAGGGTGAT+TGG 0.427544 4_3:-58005078 None:intergenic
AAACGAAACCTCTCCAGAAA+TGG 0.431051 4_3:-58005329 None:intergenic
GAAGTTGTCGTAGGAGGAGG+TGG 0.439677 4_3:-58005122 None:intergenic
TGTGCCGGAACCGGATTGCC+AGG 0.440070 4_3:-58005176 None:intergenic
TCCTCCTACGACAACTTCAT+TGG 0.441288 4_3:+58005127 Msa0650760:CDS
TATGGGTTACATTCTCGGTT+GGG 0.441433 4_3:+58007974 Msa0650760:three_prime_UTR
ATTGGTAATGCTAAACCTAA+AGG 0.441714 4_3:+58007885 Msa0650760:three_prime_UTR
ATGTTGAACCTTAAACTATA+GGG 0.442633 4_3:-58007197 None:intergenic
AAGCTCGTGGTTTCTTATGT+TGG 0.444757 4_3:+58007783 Msa0650760:three_prime_UTR
TTAGGTTTCCATGGCTGTAA+AGG 0.446726 4_3:-58007131 None:intergenic
CTCCGTTCCAACTACATCTT+CGG 0.446774 4_3:+58005220 Msa0650760:CDS
AGGTTTCCGGTTTGTGGCAA+GGG 0.449186 4_3:-58005301 None:intergenic
TTATGGGTTACATTCTCGGT+TGG 0.451487 4_3:+58007973 Msa0650760:three_prime_UTR
ACATTGTTGTTCACCAATAG+AGG 0.452089 4_3:-58007497 None:intergenic
TTGATCACTAGTTTAAAGAA+AGG 0.454168 4_3:+58005589 Msa0650760:CDS
AGTGTTGGTTGGAGGGATCC+AGG 0.454568 4_3:+58005550 Msa0650760:CDS
ATGGCGTGGGCCGGAGAGCA+GGG 0.457953 4_3:+58007591 Msa0650760:CDS
TGCCCTTGAACTACTACAAA+AGG 0.467528 4_3:-58007401 None:intergenic
AGTTTAAGGTTCAACATGCC+TGG 0.468330 4_3:+58007203 Msa0650760:CDS
AAGGAGACGTATGTGAGGTA+TGG 0.469112 4_3:+58005439 Msa0650760:CDS
AATAACCCCTTGCCACAAAC+CGG 0.470128 4_3:+58005295 Msa0650760:CDS
CCTTAGTGTGACGGTGTCCC+CGG 0.471605 4_3:-58005044 None:intergenic
CCCATGTACACAACAATCAA+TGG 0.475166 4_3:+58007428 Msa0650760:CDS
CATGATTTCCAATGCATACA+TGG 0.476850 4_3:-58007097 None:intergenic
CAACCAACACTCTCGTTAGC+AGG 0.479587 4_3:-58005538 None:intergenic
TACATTATCCCAGTATGTCC+AGG 0.482507 4_3:+58007923 Msa0650760:three_prime_UTR
GTGGCAAGGGGTTATTGTCA+TGG 0.483320 4_3:-58005288 None:intergenic
GGCAAGACAAGCAATCCATC+TGG 0.484465 4_3:+58007654 Msa0650760:CDS
GTTGGAACGGAGGTTGATGA+GGG 0.484503 4_3:-58005209 None:intergenic
AAATCATCCACCTGGCAATC+CGG 0.487160 4_3:+58005166 Msa0650760:CDS
TTGGAAATCATGAGTATGAT+TGG 0.491026 4_3:+58007108 Msa0650760:CDS
TACCTCACATACGTCTCCTT+CGG 0.495897 4_3:-58005436 None:intergenic
AAGGTTTCCGGTTTGTGGCA+AGG 0.496203 4_3:-58005302 None:intergenic
AGGAGACGTATGTGAGGTAT+GGG 0.496851 4_3:+58005440 Msa0650760:CDS
ACATCTTCGGTATCTTCCAT+TGG 0.497800 4_3:+58005233 Msa0650760:CDS
TGAGGAAAGATCGAGTCATT+GGG 0.499179 4_3:-58007695 None:intergenic
TTAGTCCAATTAGGTTTCCA+TGG 0.501745 4_3:-58007140 None:intergenic
ATCTATGAACATGGCCCCAA+AGG 0.501753 4_3:-58007529 None:intergenic
GATCAGGGTTGATTTCGGAT+TGG 0.501872 4_3:-58005258 None:intergenic
GTGGCATACCATGTATGCAT+TGG 0.503990 4_3:+58007089 Msa0650760:CDS
ATCCATCTGGAAAACAAGAC+CGG 0.506758 4_3:+58007667 Msa0650760:CDS
ATCCTTTGTCCCCTGCTCTC+CGG 0.507689 4_3:-58007601 None:intergenic
GCACCAGTACGCTTCCTCCC+TGG 0.509555 4_3:-58007941 None:intergenic
TTGCGGTGGCGCGTGTGAAG+AGG 0.510510 4_3:+58005488 Msa0650760:CDS
CTCTGAGGGTGATTGGATAC+CGG 0.512218 4_3:-58005071 None:intergenic
GGAAATTCGTCAGAACCTAC+TGG 0.514003 4_3:+58007224 Msa0650760:CDS
CAAGTGGTTATGCGTGGTTG+TGG 0.514084 4_3:+58007027 Msa0650760:CDS
CCTTCTCGCCATTTCTGGAG+AGG 0.514435 4_3:+58005321 Msa0650760:CDS
GGGGAGAGAAAATGTCATAT+GGG 0.518872 4_3:+58005460 Msa0650760:CDS
ACCGTCACACTAAGGTGGTC+CGG 0.520460 4_3:+58005052 Msa0650760:CDS
TCTATGAACATGGCCCCAAA+GGG 0.523434 4_3:-58007528 None:intergenic
CTAACGAGAGTGTTGGTTGG+AGG 0.524469 4_3:+58005542 Msa0650760:CDS
CCACAACTCTATCAAAATCC+GGG 0.525087 4_3:+58005026 Msa0650760:CDS
GTTTGTTTCGGGTAAAGGAC+CGG 0.527184 4_3:+58005348 Msa0650760:CDS
ATGATTTGGAATCAGTTGAC+AGG 0.528504 4_3:+58007369 Msa0650760:CDS
TGAACCTGTTGCAACAAAAG+TGG 0.530909 4_3:+58007070 Msa0650760:CDS
AAGTGGTTATGCGTGGTTGT+GGG 0.532637 4_3:+58007028 Msa0650760:CDS
TCTTATGTTGGAAATCATGA+TGG 0.533637 4_3:+58007795 Msa0650760:three_prime_UTR
AGTTGGAACGGAGGTTGATG+AGG 0.534951 4_3:-58005210 None:intergenic
CAAAACAGTCTTTGTATCGT+GGG 0.537627 4_3:+58007720 Msa0650760:CDS
AATGAAGTTGTCGTAGGAGG+AGG 0.537994 4_3:-58005125 None:intergenic
GAAGATACCGAAGATGTAGT+TGG 0.538252 4_3:-58005227 None:intergenic
TGGCGTGGGCCGGAGAGCAG+GGG 0.538311 4_3:+58007592 Msa0650760:CDS
ATGATTGGCCTTTACAGCCA+TGG 0.542059 4_3:+58007123 Msa0650760:CDS
CTCCGTGATGTTGAAGCTCT+AGG 0.548924 4_3:+58006960 Msa0650760:CDS
GGCCGGAGAGCAGGGGACAA+AGG 0.549021 4_3:+58007599 Msa0650760:CDS
CCATTGATTGTTGTGTACAT+GGG 0.550156 4_3:-58007428 None:intergenic
ATCTTGTGATTGGCATGGCA+GGG 0.555722 4_3:+58007633 Msa0650760:CDS
TGATTTCGGATTGGGTCCAA+TGG 0.556134 4_3:-58005249 None:intergenic
AAAGCATATCAACAGTGAAG+TGG 0.556245 4_3:+58006934 Msa0650760:CDS
GCCAATGAAGTTGTCGTAGG+AGG 0.560684 4_3:-58005128 None:intergenic
AAAGCAAGATGAATCTGCTC+CGG 0.560908 4_3:-58005367 None:intergenic
CTGCTAACGAGAGTGTTGGT+TGG 0.561825 4_3:+58005539 Msa0650760:CDS
TAACGAGAGTGTTGGTTGGA+GGG 0.568916 4_3:+58005543 Msa0650760:CDS
CCACAAGTGTAGTTATTCAG+TGG 0.569232 4_3:-58007566 None:intergenic
CCGGGGACACCGTCACACTA+AGG 0.569874 4_3:+58005044 Msa0650760:CDS
ATCGAGTCATTGGGATGACC+CGG 0.570194 4_3:-58007686 None:intergenic
TGTCCAGGGAGGAAGCGTAC+TGG 0.570210 4_3:+58007938 Msa0650760:three_prime_UTR
ATCTCTCGTATCTATGAACA+TGG 0.572154 4_3:-58007538 None:intergenic
CCTCTCCAGAAATGGCGAGA+AGG 0.575084 4_3:-58005321 None:intergenic
TGTCATGGTCATAACGATCA+GGG 0.575204 4_3:-58005273 None:intergenic
CAACTCATAGCTTTGTGTCA+AGG 0.575696 4_3:+58006823 Msa0650760:CDS
CCACTGAATAACTACACTTG+TGG 0.577944 4_3:+58007566 Msa0650760:CDS
CTTGTGGAAATGGCGTGGGC+CGG 0.578247 4_3:+58007582 Msa0650760:CDS
TCGGTTGGGTTATACATGCA+AGG 0.580242 4_3:+58007988 Msa0650760:three_prime_UTR
TTCCTAGAGCTTCAACATCA+CGG 0.580505 4_3:-58006962 None:intergenic
CGTTCATCTTGTGATTGGCA+TGG 0.581543 4_3:+58007628 Msa0650760:CDS
ATGTCATATGGGAGGTCTTG+CGG 0.583227 4_3:+58005471 Msa0650760:CDS
TAGAGGAAGAGTATTGTGCA+AGG 0.584858 4_3:-58004944 None:intergenic
GAACATGGCCCCAAAGGGCA+AGG 0.584999 4_3:-58007523 None:intergenic
GGATGAAGAAGATGCTATGC+GGG 0.585911 4_3:+58005393 Msa0650760:CDS
GTAGAAACACTCCTGGACTC+TGG 0.586121 4_3:-58008017 None:intergenic
GCTTCCTCCCTGGACATACT+GGG 0.586632 4_3:-58007931 None:intergenic
ACTAATTATGGAAAAGATGG+AGG 0.587578 4_3:+58007158 Msa0650760:CDS
TACACTTGTGGAAATGGCGT+GGG 0.587976 4_3:+58007578 Msa0650760:CDS
CCAAAACAGTCTTTGTATCG+TGG 0.588649 4_3:+58007719 Msa0650760:CDS
GCTACGAAGTCTAGCTCTGA+GGG 0.589676 4_3:-58005085 None:intergenic
CCGATGTGTGACACAAAAGC+GGG 0.592792 4_3:-58006990 None:intergenic
TACCGAAGATGTAGTTGGAA+CGG 0.595020 4_3:-58005222 None:intergenic
AATTATGGAAAAGATGGAGG+TGG 0.595238 4_3:+58007161 Msa0650760:CDS
CTACACTTGTGGAAATGGCG+TGG 0.595264 4_3:+58007577 Msa0650760:CDS
AAAGCATCATGTATATAACC+TGG 0.595486 4_3:-58005568 None:intergenic
TCCATCTGGAAAACAAGACC+GGG 0.597952 4_3:+58007668 Msa0650760:CDS
GGTTTCCGGTTTGTGGCAAG+GGG 0.598797 4_3:-58005300 None:intergenic
CGTGTGAGTAGAAACACTCC+TGG 0.600020 4_3:-58008024 None:intergenic
AGGAAGCGTACTGGTGCTTG+GGG 0.600088 4_3:+58007947 Msa0650760:three_prime_UTR
GTATGTGACGAGGGATCCGA+AGG 0.606007 4_3:+58005420 Msa0650760:CDS
CACAACAATCAATGGAACCA+AGG 0.611304 4_3:+58007436 Msa0650760:CDS
TCCTCTCTAAATCATCCACC+TGG 0.615171 4_3:+58005158 Msa0650760:CDS
TCCGATGTGTGACACAAAAG+CGG 0.615605 4_3:-58006991 None:intergenic
CGCGTGTGAAGAGGTATGAG+AGG 0.617971 4_3:+58005497 Msa0650760:CDS
AAAACAGTCTTTGTATCGTG+GGG 0.618337 4_3:+58007721 Msa0650760:CDS
TGGAACGGAGGTTGATGAGG+GGG 0.618753 4_3:-58005207 None:intergenic
GTTTGGGTACATTAGACTAG+TGG 0.624624 4_3:+58007749 Msa0650760:three_prime_UTR
GTAGCCAATGAAGTTGTCGT+AGG 0.625292 4_3:-58005131 None:intergenic
ACCGGACCACCTTAGTGTGA+CGG 0.627721 4_3:-58005053 None:intergenic
TTGTCGTAGGAGGAGGTGGG+TGG 0.632479 4_3:-58005118 None:intergenic
AAAGATGGAGGTGGTGAGTG+CGG 0.636261 4_3:+58007170 Msa0650760:CDS
GCCAGGTGGATGATTTAGAG+AGG 0.636449 4_3:-58005159 None:intergenic
GCTGGAGCTATAGTTCCAGT+AGG 0.636710 4_3:-58007239 None:intergenic
ACATTATCCCAGTATGTCCA+GGG 0.637529 4_3:+58007924 Msa0650760:three_prime_UTR
AAGTTGTCGTAGGAGGAGGT+GGG 0.638967 4_3:-58005121 None:intergenic
GCGGGTTATGTATGTGACGA+GGG 0.644902 4_3:+58005411 Msa0650760:CDS
TTGGAACGGAGGTTGATGAG+GGG 0.645080 4_3:-58005208 None:intergenic
GGAGACATTAGTTACGCAAG+TGG 0.646032 4_3:+58007011 Msa0650760:CDS
AAACAGTCTTTGTATCGTGG+GGG 0.648873 4_3:+58007722 Msa0650760:CDS
AATGTGAACCTTGCCCTTTG+GGG 0.650716 4_3:+58007515 Msa0650760:CDS
TGCGGGTTATGTATGTGACG+AGG 0.651449 4_3:+58005410 Msa0650760:CDS
GGGACACCGTCACACTAAGG+TGG 0.653790 4_3:+58005047 Msa0650760:CDS
AACAGTCTTTGTATCGTGGG+GGG 0.656017 4_3:+58007723 Msa0650760:CDS
TGCTACGAAGTCTAGCTCTG+AGG 0.656528 4_3:-58005086 None:intergenic
GTTACGCAAGTGGTTATGCG+TGG 0.658494 4_3:+58007021 Msa0650760:CDS
CATGCCAATCACAAGATGAA+CGG 0.659360 4_3:-58007627 None:intergenic
GCGTGTGAAGAGGTATGAGA+GGG 0.660850 4_3:+58005498 Msa0650760:CDS
GGCTACAAAGCAGAAGCTCG+TGG 0.661109 4_3:+58007770 Msa0650760:three_prime_UTR
GCTCCTGCTAACGAGAGTGT+TGG 0.663854 4_3:+58005535 Msa0650760:CDS
CACAACTCTATCAAAATCCG+GGG 0.669749 4_3:+58005027 Msa0650760:CDS
CGGATGAAGAAGATGCTATG+CGG 0.672413 4_3:+58005392 Msa0650760:CDS
ATCCGAAGGAGACGTATGTG+AGG 0.673765 4_3:+58005434 Msa0650760:CDS
GAGAGAAAATGTCATATGGG+AGG 0.677448 4_3:+58005463 Msa0650760:CDS
CGAAGATGTAGTTGGAACGG+AGG 0.680198 4_3:-58005219 None:intergenic
TCATATGGGAGGTCTTGCGG+TGG 0.686577 4_3:+58005474 Msa0650760:CDS
TGTTGGAAATCATGATGGTG+AGG 0.687796 4_3:+58007800 Msa0650760:three_prime_UTR
GCCGGAACCGGATTGCCAGG+TGG 0.689201 4_3:-58005173 None:intergenic
GGAGACGTATGTGAGGTATG+GGG 0.695565 4_3:+58005441 Msa0650760:CDS
TGGTGAGGTGCATGACACAA+TGG 0.697263 4_3:+58007815 Msa0650760:three_prime_UTR
CTAGAGCTTCAACATCACGG+AGG 0.777257 4_3:-58006959 None:intergenic
TTATCCCAGTATGTCCAGGG+AGG 0.778942 4_3:+58007927 Msa0650760:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTATTTTTTAATAATA+AGG + chr4_3:58006560-58006579 Msa0650760:intron 0.0%
!! AATAATGAAAGTAAAAAACA+AGG - chr4_3:58005709-58005728 None:intergenic 15.0%
!!! TTTTGCTTAATTTTATTGAA+TGG + chr4_3:58006329-58006348 Msa0650760:intron 15.0%
!!! TTTGCTTAATTTTATTGAAT+GGG + chr4_3:58006330-58006349 Msa0650760:intron 15.0%
!! AAAATTATGATAGAAAACAA+AGG + chr4_3:58006620-58006639 Msa0650760:intron 15.0%
!!! AAAACAAAATCATTGTTTTA+AGG + chr4_3:58006683-58006702 Msa0650760:intron 15.0%
!! TTTCATTATTACTTTGTGTT+TGG + chr4_3:58005719-58005738 Msa0650760:intron 20.0%
!!! AAAATACCTCGATATTTTTT+TGG + chr4_3:58005960-58005979 Msa0650760:intron 20.0%
!!! AAATACCTCGATATTTTTTT+GGG + chr4_3:58005961-58005980 Msa0650760:intron 20.0%
!!! TGGAAAAGATTTTTAAGTAT+TGG + chr4_3:58006413-58006432 Msa0650760:intron 20.0%
!! TTGTATATCCAAATATAGTT+GGG - chr4_3:58006473-58006492 None:intergenic 20.0%
!! ATTGTATATCCAAATATAGT+TGG - chr4_3:58006474-58006493 None:intergenic 20.0%
!! TTGTATATCCAAATATAGTT+GGG - chr4_3:58006473-58006492 None:intergenic 20.0%
!! ATTGTATATCCAAATATAGT+TGG - chr4_3:58006474-58006493 None:intergenic 20.0%
!! TCTTTATTACTCATTTGATA+TGG + chr4_3:58007271-58007290 Msa0650760:CDS 20.0%
!! CTTTATTACTCATTTGATAT+GGG + chr4_3:58007272-58007291 Msa0650760:CDS 20.0%
! TGAAGATTATTGAAAGATAG+AGG - chr4_3:58004964-58004983 None:intergenic 25.0%
!! TTGATCACTAGTTTAAAGAA+AGG + chr4_3:58005589-58005608 Msa0650760:CDS 25.0%
! ATGAAAGTAAAAAACAAGGT+AGG - chr4_3:58005705-58005724 None:intergenic 25.0%
! ATTATTACTTTGTGTTTGGT+TGG + chr4_3:58005723-58005742 Msa0650760:intron 25.0%
!! GGTAATGGTTTAAAGTTTAA+AGG + chr4_3:58005795-58005814 Msa0650760:intron 25.0%
!!! TTTGATTTGATAAAGAGACA+AGG + chr4_3:58005989-58006008 Msa0650760:intron 25.0%
! CACTAAATCATAAGAAAACT+TGG + chr4_3:58006113-58006132 Msa0650760:intron 25.0%
!!! ATTTTTGATAGTTTGCGTTA+TGG - chr4_3:58006223-58006242 None:intergenic 25.0%
!!! AAGATTTTTAAGTATTGGTC+TGG + chr4_3:58006418-58006437 Msa0650760:intron 25.0%
! TCATGTAATCGTTTAGTATA+TGG + chr4_3:58006732-58006751 Msa0650760:intron 25.0%
!! GAAACAATAGCTTTCATTTT+TGG + chr4_3:58006861-58006880 Msa0650760:CDS 25.0%
! GTACGCAAAAATGTATTATA+TGG - chr4_3:58006906-58006925 None:intergenic 25.0%
! AAACCTAATTGGACTAATTA+TGG + chr4_3:58007146-58007165 Msa0650760:CDS 25.0%
! TTTCCATAATTAGTCCAATT+AGG - chr4_3:58007152-58007171 None:intergenic 25.0%
! ATGTTGAACCTTAAACTATA+GGG - chr4_3:58007200-58007219 None:intergenic 25.0%
!!! TAATAAAGATTTCTAGTTGC+TGG - chr4_3:58007260-58007279 None:intergenic 25.0%
! GGTAATGTTAATGTTGATAT+TGG + chr4_3:58007867-58007886 Msa0650760:three_prime_UTR 25.0%
! AAAGCATCATGTATATAACC+TGG - chr4_3:58005571-58005590 None:intergenic 30.0%
AGGAAGAAGATACTACTATA+AGG + chr4_3:58005609-58005628 Msa0650760:CDS 30.0%
CAAGGTAATCAAAATTAGAC+CGG + chr4_3:58005750-58005769 Msa0650760:intron 30.0%
! CATTAAACATGTGTTGGTAA+TGG + chr4_3:58005774-58005793 Msa0650760:intron 30.0%
GGAGAACTAAAATCGTATTT+CGG - chr4_3:58006247-58006266 None:intergenic 30.0%
!! ATATAGTTGGGTTCTAAACA+TGG - chr4_3:58006513-58006532 None:intergenic 30.0%
! GTTTAGAACCCAACTATATT+TGG + chr4_3:58006514-58006533 Msa0650760:intron 30.0%
GATTACATGAACCAGATTTA+GGG - chr4_3:58006722-58006741 None:intergenic 30.0%
! TACCAGGTTGGAAATAAAAA+TGG + chr4_3:58006789-58006808 Msa0650760:intron 30.0%
TTGGAAATAAAAATGGAGGT+TGG + chr4_3:58006796-58006815 Msa0650760:CDS 30.0%
TTGGAAATCATGAGTATGAT+TGG + chr4_3:58007108-58007127 Msa0650760:CDS 30.0%
TGGACTAATTATGGAAAAGA+TGG + chr4_3:58007155-58007174 Msa0650760:CDS 30.0%
ACTAATTATGGAAAAGATGG+AGG + chr4_3:58007158-58007177 Msa0650760:CDS 30.0%
CATGTTGAACCTTAAACTAT+AGG - chr4_3:58007201-58007220 None:intergenic 30.0%
TAACTTCTTGAAGCATGATT+TGG + chr4_3:58007355-58007374 Msa0650760:CDS 30.0%
AATAACTACACTTGTGGAAA+TGG + chr4_3:58007572-58007591 Msa0650760:CDS 30.0%
TCTTATGTTGGAAATCATGA+TGG + chr4_3:58007795-58007814 Msa0650760:three_prime_UTR 30.0%
!! ATTGGTAATGCTAAACCTAA+AGG + chr4_3:58007885-58007904 Msa0650760:three_prime_UTR 30.0%
! ATAACGATCAGGGTTGATTT+CGG - chr4_3:58005266-58005285 None:intergenic 35.0%
TTGTGTTTGGTTGGAAAACA+AGG + chr4_3:58005732-58005751 Msa0650760:intron 35.0%
ACCAACACATGTTTAATGTC+CGG - chr4_3:58005772-58005791 None:intergenic 35.0%
!! ACATGTGTTGGTAATGGTAA+TGG + chr4_3:58005780-58005799 Msa0650760:intron 35.0%
ACACACCCAAAAAAATATCG+AGG - chr4_3:58005969-58005988 None:intergenic 35.0%
! ACCCTGTTTGATTTACAAAC+TGG + chr4_3:58006032-58006051 Msa0650760:intron 35.0%
GCCAGTTTGTAAATCAAACA+GGG - chr4_3:58006036-58006055 None:intergenic 35.0%
TTGATTTACAAACTGGCCAT+TGG + chr4_3:58006039-58006058 Msa0650760:intron 35.0%
!!! ACTTGTTTTGTCTAGTCCAA+TGG - chr4_3:58006058-58006077 None:intergenic 35.0%
TCATAAGAAAACTTGGCCAT+TGG + chr4_3:58006120-58006139 Msa0650760:intron 35.0%
!!! ACTTGTTTTGTCTAGTCCAA+TGG - chr4_3:58006058-58006077 None:intergenic 35.0%
CCAAATATAGTTGGGTTCTA+CGG - chr4_3:58006465-58006484 None:intergenic 35.0%
CGATTACATGAACCAGATTT+AGG - chr4_3:58006723-58006742 None:intergenic 35.0%
CATCACTCAAGTTAAGTTTC+TGG - chr4_3:58006761-58006780 None:intergenic 35.0%
! CAGAAACTTAACTTGAGTGA+TGG + chr4_3:58006759-58006778 Msa0650760:intron 35.0%
!! CTCCATTTTTATTTCCAACC+TGG - chr4_3:58006794-58006813 None:intergenic 35.0%
! CAGGTTGGAAATAAAAATGG+AGG + chr4_3:58006792-58006811 Msa0650760:intron 35.0%
!!! AGCTTTCATTTTTGGTGACA+TGG + chr4_3:58006869-58006888 Msa0650760:CDS 35.0%
!!! GCTTTCATTTTTGGTGACAT+GGG + chr4_3:58006870-58006889 Msa0650760:CDS 35.0%
AAAGCATATCAACAGTGAAG+TGG + chr4_3:58006934-58006953 Msa0650760:CDS 35.0%
CATGATTTCCAATGCATACA+TGG - chr4_3:58007100-58007119 None:intergenic 35.0%
TTAGTCCAATTAGGTTTCCA+TGG - chr4_3:58007143-58007162 None:intergenic 35.0%
AATTATGGAAAAGATGGAGG+TGG + chr4_3:58007161-58007180 Msa0650760:CDS 35.0%
! ACAGAGACCAATTTTCTTCT+TGG + chr4_3:58007320-58007339 Msa0650760:CDS 35.0%
! CAGAGACCAATTTTCTTCTT+GGG + chr4_3:58007321-58007340 Msa0650760:CDS 35.0%
CATGCTTCAAGAAGTTATAC+TGG - chr4_3:58007352-58007371 None:intergenic 35.0%
! ATGATTTGGAATCAGTTGAC+AGG + chr4_3:58007369-58007388 Msa0650760:CDS 35.0%
! ACTCCTTTTGTAGTAGTTCA+AGG + chr4_3:58007398-58007417 Msa0650760:CDS 35.0%
! CTCCTTTTGTAGTAGTTCAA+GGG + chr4_3:58007399-58007418 Msa0650760:CDS 35.0%
! CCATTGATTGTTGTGTACAT+GGG - chr4_3:58007431-58007450 None:intergenic 35.0%
! TCCATTGATTGTTGTGTACA+TGG - chr4_3:58007432-58007451 None:intergenic 35.0%
! ACATTGTTGTTCACCAATAG+AGG - chr4_3:58007500-58007519 None:intergenic 35.0%
ATCTCTCGTATCTATGAACA+TGG - chr4_3:58007541-58007560 None:intergenic 35.0%
TATACCGTTCATCTTGTGAT+TGG + chr4_3:58007623-58007642 Msa0650760:CDS 35.0%
!!! TACAAAGACTGTTTTGGTTG+AGG - chr4_3:58007716-58007735 None:intergenic 35.0%
CAAAACAGTCTTTGTATCGT+GGG + chr4_3:58007720-58007739 Msa0650760:CDS 35.0%
AAAACAGTCTTTGTATCGTG+GGG + chr4_3:58007721-58007740 Msa0650760:CDS 35.0%
! GCATGACACAATGGAAATTT+TGG + chr4_3:58007824-58007843 Msa0650760:three_prime_UTR 35.0%
!! ACAATGGAAATTTTGGCATC+TGG + chr4_3:58007831-58007850 Msa0650760:three_prime_UTR 35.0%
GCTTCTTCAATCAAACCTTT+AGG - chr4_3:58007903-58007922 None:intergenic 35.0%
!! TAGAGGAAGAGTATTGTGCA+AGG - chr4_3:58004947-58004966 None:intergenic 40.0%
!! GGATTTTGATAGAGTTGTGG+GGG - chr4_3:58005026-58005045 None:intergenic 40.0%
!! CGGATTTTGATAGAGTTGTG+GGG - chr4_3:58005027-58005046 None:intergenic 40.0%
!! CCGGATTTTGATAGAGTTGT+GGG - chr4_3:58005028-58005047 None:intergenic 40.0%
CCCACAACTCTATCAAAATC+CGG + chr4_3:58005025-58005044 Msa0650760:CDS 40.0%
CCACAACTCTATCAAAATCC+GGG + chr4_3:58005026-58005045 Msa0650760:CDS 40.0%
CACAACTCTATCAAAATCCG+GGG + chr4_3:58005027-58005046 Msa0650760:CDS 40.0%
TGGATGATTTAGAGAGGAAG+AGG - chr4_3:58005156-58005175 None:intergenic 40.0%
TACCGAAGATGTAGTTGGAA+CGG - chr4_3:58005225-58005244 None:intergenic 40.0%
GAAGATACCGAAGATGTAGT+TGG - chr4_3:58005230-58005249 None:intergenic 40.0%
ACATCTTCGGTATCTTCCAT+TGG + chr4_3:58005233-58005252 Msa0650760:CDS 40.0%
! ATCAGGGTTGATTTCGGATT+GGG - chr4_3:58005260-58005279 None:intergenic 40.0%
TGTCATGGTCATAACGATCA+GGG - chr4_3:58005276-58005295 None:intergenic 40.0%
TTGTCATGGTCATAACGATC+AGG - chr4_3:58005277-58005296 None:intergenic 40.0%
AAACGAAACCTCTCCAGAAA+TGG - chr4_3:58005332-58005351 None:intergenic 40.0%
TGGAGAGGTTTCGTTTGTTT+CGG + chr4_3:58005336-58005355 Msa0650760:CDS 40.0%
! GTTTCGTTTGTTTCGGGTAA+AGG + chr4_3:58005343-58005362 Msa0650760:CDS 40.0%
AAAGCAAGATGAATCTGCTC+CGG - chr4_3:58005370-58005389 None:intergenic 40.0%
!! GCAGATTCATCTTGCTTTTG+CGG + chr4_3:58005372-58005391 Msa0650760:CDS 40.0%
TGGGGAGAGAAAATGTCATA+TGG + chr4_3:58005459-58005478 Msa0650760:CDS 40.0%
GGGGAGAGAAAATGTCATAT+GGG + chr4_3:58005460-58005479 Msa0650760:CDS 40.0%
GAGAGAAAATGTCATATGGG+AGG + chr4_3:58005463-58005482 Msa0650760:CDS 40.0%
ACCGGACATTAAACATGTGT+TGG + chr4_3:58005768-58005787 Msa0650760:intron 40.0%
GGCCAGTTTGTAAATCAAAC+AGG - chr4_3:58006037-58006056 None:intergenic 40.0%
ATCATCGTCTAGTGAGAAAG+AGG + chr4_3:58006354-58006373 Msa0650760:intron 40.0%
TGAGAAAGAGGATCATGAGT+TGG + chr4_3:58006366-58006385 Msa0650760:intron 40.0%
GAGAAAGAGGATCATGAGTT+GGG + chr4_3:58006367-58006386 Msa0650760:intron 40.0%
!! TCATCGATTGCTGTTTGAGT+TGG + chr4_3:58006393-58006412 Msa0650760:intron 40.0%
CCGTAGAACCCAACTATATT+TGG + chr4_3:58006462-58006481 Msa0650760:intron 40.0%
AAACAAACACGCCCTAAATC+TGG + chr4_3:58006708-58006727 Msa0650760:intron 40.0%
!! CAACTCATAGCTTTGTGTCA+AGG + chr4_3:58006823-58006842 Msa0650760:CDS 40.0%
TTCCTAGAGCTTCAACATCA+CGG - chr4_3:58006965-58006984 None:intergenic 40.0%
TGAACCTGTTGCAACAAAAG+TGG + chr4_3:58007070-58007089 Msa0650760:CDS 40.0%
!!! TATGCCACTTTTGTTGCAAC+AGG - chr4_3:58007077-58007096 None:intergenic 40.0%
TTAGGTTTCCATGGCTGTAA+AGG - chr4_3:58007134-58007153 None:intergenic 40.0%
TACAGCCATGGAAACCTAAT+TGG + chr4_3:58007135-58007154 Msa0650760:CDS 40.0%
! AGTTTAAGGTTCAACATGCC+TGG + chr4_3:58007203-58007222 Msa0650760:CDS 40.0%
GTTGCTCCCAAGAAGAAAAT+TGG - chr4_3:58007330-58007349 None:intergenic 40.0%
TGCCCTTGAACTACTACAAA+AGG - chr4_3:58007404-58007423 None:intergenic 40.0%
CCCATGTACACAACAATCAA+TGG + chr4_3:58007428-58007447 Msa0650760:CDS 40.0%
CACAACAATCAATGGAACCA+AGG + chr4_3:58007436-58007455 Msa0650760:CDS 40.0%
AGAAAAGATGCTTGAGCACT+TGG + chr4_3:58007472-58007491 Msa0650760:CDS 40.0%
CCACAAGTGTAGTTATTCAG+TGG - chr4_3:58007569-58007588 None:intergenic 40.0%
CCACTGAATAACTACACTTG+TGG + chr4_3:58007566-58007585 Msa0650760:CDS 40.0%
CATGCCAATCACAAGATGAA+CGG - chr4_3:58007630-58007649 None:intergenic 40.0%
ATCCATCTGGAAAACAAGAC+CGG + chr4_3:58007667-58007686 Msa0650760:CDS 40.0%
TGAGGAAAGATCGAGTCATT+GGG - chr4_3:58007698-58007717 None:intergenic 40.0%
TTGAGGAAAGATCGAGTCAT+TGG - chr4_3:58007699-58007718 None:intergenic 40.0%
! CCACGATACAAAGACTGTTT+TGG - chr4_3:58007722-58007741 None:intergenic 40.0%
CCAAAACAGTCTTTGTATCG+TGG + chr4_3:58007719-58007738 Msa0650760:CDS 40.0%
AAACAGTCTTTGTATCGTGG+GGG + chr4_3:58007722-58007741 Msa0650760:CDS 40.0%
GTTTGGGTACATTAGACTAG+TGG + chr4_3:58007749-58007768 Msa0650760:three_prime_UTR 40.0%
AAGCTCGTGGTTTCTTATGT+TGG + chr4_3:58007783-58007802 Msa0650760:three_prime_UTR 40.0%
! TGTTGGAAATCATGATGGTG+AGG + chr4_3:58007800-58007819 Msa0650760:three_prime_UTR 40.0%
! GCATCTGGAGAACTTTTCAA+TGG + chr4_3:58007846-58007865 Msa0650760:three_prime_UTR 40.0%
TACATTATCCCAGTATGTCC+AGG + chr4_3:58007923-58007942 Msa0650760:three_prime_UTR 40.0%
ACATTATCCCAGTATGTCCA+GGG + chr4_3:58007924-58007943 Msa0650760:three_prime_UTR 40.0%
! GCCTTTATGGGTTACATTCT+CGG + chr4_3:58007969-58007988 Msa0650760:three_prime_UTR 40.0%
ACCGAGAATGTAACCCATAA+AGG - chr4_3:58007973-58007992 None:intergenic 40.0%
! TTATGGGTTACATTCTCGGT+TGG + chr4_3:58007973-58007992 Msa0650760:three_prime_UTR 40.0%
! TATGGGTTACATTCTCGGTT+GGG + chr4_3:58007974-58007993 Msa0650760:three_prime_UTR 40.0%
!!! GGGTTTTGATTGGACAAGGT+TGG - chr4_3:58004990-58005009 None:intergenic 45.0%
TGATAGAGTTGTGGGGGTTA+CGG - chr4_3:58005020-58005039 None:intergenic 45.0%
!! CCCGGATTTTGATAGAGTTG+TGG - chr4_3:58005029-58005048 None:intergenic 45.0%
AATGAAGTTGTCGTAGGAGG+AGG - chr4_3:58005128-58005147 None:intergenic 45.0%
TCCTCCTACGACAACTTCAT+TGG + chr4_3:58005127-58005146 Msa0650760:CDS 45.0%
GTAGCCAATGAAGTTGTCGT+AGG - chr4_3:58005134-58005153 None:intergenic 45.0%
TCCTCTCTAAATCATCCACC+TGG + chr4_3:58005158-58005177 Msa0650760:CDS 45.0%
AAATCATCCACCTGGCAATC+CGG + chr4_3:58005166-58005185 Msa0650760:CDS 45.0%
CTCCGTTCCAACTACATCTT+CGG + chr4_3:58005220-58005239 Msa0650760:CDS 45.0%
! TGATTTCGGATTGGGTCCAA+TGG - chr4_3:58005252-58005271 None:intergenic 45.0%
! GATCAGGGTTGATTTCGGAT+TGG - chr4_3:58005261-58005280 None:intergenic 45.0%
AATAACCCCTTGCCACAAAC+CGG + chr4_3:58005295-58005314 Msa0650760:CDS 45.0%
AGAAATGGCGAGAAGGTTTC+CGG - chr4_3:58005317-58005336 None:intergenic 45.0%
GGAGAGGTTTCGTTTGTTTC+GGG + chr4_3:58005337-58005356 Msa0650760:CDS 45.0%
! GTTTGTTTCGGGTAAAGGAC+CGG + chr4_3:58005348-58005367 Msa0650760:CDS 45.0%
CGGATGAAGAAGATGCTATG+CGG + chr4_3:58005392-58005411 Msa0650760:CDS 45.0%
GGATGAAGAAGATGCTATGC+GGG + chr4_3:58005393-58005412 Msa0650760:CDS 45.0%
TACCTCACATACGTCTCCTT+CGG - chr4_3:58005439-58005458 None:intergenic 45.0%
AAGGAGACGTATGTGAGGTA+TGG + chr4_3:58005439-58005458 Msa0650760:CDS 45.0%
AGGAGACGTATGTGAGGTAT+GGG + chr4_3:58005440-58005459 Msa0650760:CDS 45.0%
ATGTCATATGGGAGGTCTTG+CGG + chr4_3:58005471-58005490 Msa0650760:CDS 45.0%
!! TAACGAGAGTGTTGGTTGGA+GGG + chr4_3:58005543-58005562 Msa0650760:CDS 45.0%
! TACGGTTGACTAGCTGACAA+GGG - chr4_3:58006447-58006466 None:intergenic 45.0%
TCCGATGTGTGACACAAAAG+CGG - chr4_3:58006994-58007013 None:intergenic 45.0%
GGAGACATTAGTTACGCAAG+TGG + chr4_3:58007011-58007030 Msa0650760:CDS 45.0%
AAGTGGTTATGCGTGGTTGT+GGG + chr4_3:58007028-58007047 Msa0650760:CDS 45.0%
GTGGCATACCATGTATGCAT+TGG + chr4_3:58007089-58007108 Msa0650760:CDS 45.0%
!! ATGATTGGCCTTTACAGCCA+TGG + chr4_3:58007123-58007142 Msa0650760:CDS 45.0%
GCGGTGTACCCTATAGTTTA+AGG + chr4_3:58007189-58007208 Msa0650760:CDS 45.0%
! GTAGGTTCTGACGAATTTCC+AGG - chr4_3:58007224-58007243 None:intergenic 45.0%
GGAAATTCGTCAGAACCTAC+TGG + chr4_3:58007224-58007243 Msa0650760:CDS 45.0%
TTCTCTCAACGCAACATCCT+TGG - chr4_3:58007456-58007475 None:intergenic 45.0%
TGAGCACTTGGAACCTCTAT+TGG + chr4_3:58007484-58007503 Msa0650760:CDS 45.0%
ACAATGTGAACCTTGCCCTT+TGG + chr4_3:58007513-58007532 Msa0650760:CDS 45.0%
CAATGTGAACCTTGCCCTTT+GGG + chr4_3:58007514-58007533 Msa0650760:CDS 45.0%
AATGTGAACCTTGCCCTTTG+GGG + chr4_3:58007515-58007534 Msa0650760:CDS 45.0%
TCTATGAACATGGCCCCAAA+GGG - chr4_3:58007531-58007550 None:intergenic 45.0%
ATCTATGAACATGGCCCCAA+AGG - chr4_3:58007532-58007551 None:intergenic 45.0%
TACACTTGTGGAAATGGCGT+GGG + chr4_3:58007578-58007597 Msa0650760:CDS 45.0%
CGTTCATCTTGTGATTGGCA+TGG + chr4_3:58007628-58007647 Msa0650760:CDS 45.0%
ATCTTGTGATTGGCATGGCA+GGG + chr4_3:58007633-58007652 Msa0650760:CDS 45.0%
TCCATCTGGAAAACAAGACC+GGG + chr4_3:58007668-58007687 Msa0650760:CDS 45.0%
AACAGTCTTTGTATCGTGGG+GGG + chr4_3:58007723-58007742 Msa0650760:CDS 45.0%
TCGGTTGGGTTATACATGCA+AGG + chr4_3:58007988-58008007 Msa0650760:three_prime_UTR 45.0%
CAAGGAAAAAACCAGAGTCC+AGG + chr4_3:58008006-58008025 Msa0650760:three_prime_UTR 45.0%
!!! ATATACATTTTAAAAATTAT+AGG - chr4_3:58005823-58005842 None:intergenic 5.0%
!! ATAAAAAAAAAATATATTTG+AGG + chr4_3:58005911-58005930 Msa0650760:intron 5.0%
!!! ATTTATAATATTTTTTATGT+TGG - chr4_3:58006268-58006287 None:intergenic 5.0%
!!! GGGAGGGTTTTGATTGGACA+AGG - chr4_3:58004994-58005013 None:intergenic 50.0%
!! CGGTTAGGGAGGGTTTTGAT+TGG - chr4_3:58005000-58005019 None:intergenic 50.0%
AGTTGTGGGGGTTACGGTTA+GGG - chr4_3:58005014-58005033 None:intergenic 50.0%
! CTCTGAGGGTGATTGGATAC+CGG - chr4_3:58005074-58005093 None:intergenic 50.0%
!! AGTCTAGCTCTGAGGGTGAT+TGG - chr4_3:58005081-58005100 None:intergenic 50.0%
!! GCTACGAAGTCTAGCTCTGA+GGG - chr4_3:58005088-58005107 None:intergenic 50.0%
!! TGCTACGAAGTCTAGCTCTG+AGG - chr4_3:58005089-58005108 None:intergenic 50.0%
AAGTTGTCGTAGGAGGAGGT+GGG - chr4_3:58005124-58005143 None:intergenic 50.0%
GCCAATGAAGTTGTCGTAGG+AGG - chr4_3:58005131-58005150 None:intergenic 50.0%
GCCAGGTGGATGATTTAGAG+AGG - chr4_3:58005162-58005181 None:intergenic 50.0%
!! TTGGAACGGAGGTTGATGAG+GGG - chr4_3:58005211-58005230 None:intergenic 50.0%
!! GTTGGAACGGAGGTTGATGA+GGG - chr4_3:58005212-58005231 None:intergenic 50.0%
!! AGTTGGAACGGAGGTTGATG+AGG - chr4_3:58005213-58005232 None:intergenic 50.0%
CGAAGATGTAGTTGGAACGG+AGG - chr4_3:58005222-58005241 None:intergenic 50.0%
GTGGCAAGGGGTTATTGTCA+TGG - chr4_3:58005291-58005310 None:intergenic 50.0%
! AGGTTTCCGGTTTGTGGCAA+GGG - chr4_3:58005304-58005323 None:intergenic 50.0%
! AAGGTTTCCGGTTTGTGGCA+AGG - chr4_3:58005305-58005324 None:intergenic 50.0%
GGAAACCTTCTCGCCATTTC+TGG + chr4_3:58005316-58005335 Msa0650760:CDS 50.0%
! TGCGGGTTATGTATGTGACG+AGG + chr4_3:58005410-58005429 Msa0650760:CDS 50.0%
! GCGGGTTATGTATGTGACGA+GGG + chr4_3:58005411-58005430 Msa0650760:CDS 50.0%
ATCCGAAGGAGACGTATGTG+AGG + chr4_3:58005434-58005453 Msa0650760:CDS 50.0%
GGAGACGTATGTGAGGTATG+GGG + chr4_3:58005441-58005460 Msa0650760:CDS 50.0%
! GCGTGTGAAGAGGTATGAGA+GGG + chr4_3:58005498-58005517 Msa0650760:CDS 50.0%
CAACCAACACTCTCGTTAGC+AGG - chr4_3:58005541-58005560 None:intergenic 50.0%
!! CTGCTAACGAGAGTGTTGGT+TGG + chr4_3:58005539-58005558 Msa0650760:CDS 50.0%
!! CTAACGAGAGTGTTGGTTGG+AGG + chr4_3:58005542-58005561 Msa0650760:CDS 50.0%
! CTACGGTTGACTAGCTGACA+AGG - chr4_3:58006448-58006467 None:intergenic 50.0%
!! GAGTGATGGTGTTGTGTACC+AGG + chr4_3:58006773-58006792 Msa0650760:intron 50.0%
!! GATGGTGTTGTGTACCAGGT+TGG + chr4_3:58006777-58006796 Msa0650760:intron 50.0%
CTAGAGCTTCAACATCACGG+AGG - chr4_3:58006962-58006981 None:intergenic 50.0%
CTCCGTGATGTTGAAGCTCT+AGG + chr4_3:58006960-58006979 Msa0650760:CDS 50.0%
CCGATGTGTGACACAAAAGC+GGG - chr4_3:58006993-58007012 None:intergenic 50.0%
!! CCCGCTTTTGTGTCACACAT+CGG + chr4_3:58006990-58007009 Msa0650760:CDS 50.0%
GTTACGCAAGTGGTTATGCG+TGG + chr4_3:58007021-58007040 Msa0650760:CDS 50.0%
CAAGTGGTTATGCGTGGTTG+TGG + chr4_3:58007027-58007046 Msa0650760:CDS 50.0%
AAAGATGGAGGTGGTGAGTG+CGG + chr4_3:58007170-58007189 Msa0650760:CDS 50.0%
GCTGGAGCTATAGTTCCAGT+AGG - chr4_3:58007242-58007261 None:intergenic 50.0%
CTACACTTGTGGAAATGGCG+TGG + chr4_3:58007577-58007596 Msa0650760:CDS 50.0%
CATCTTGTGATTGGCATGGC+AGG + chr4_3:58007632-58007651 Msa0650760:CDS 50.0%
GGCAAGACAAGCAATCCATC+TGG + chr4_3:58007654-58007673 Msa0650760:CDS 50.0%
!!! ACCCGGTCTTGTTTTCCAGA+TGG - chr4_3:58007672-58007691 None:intergenic 50.0%
ATCGAGTCATTGGGATGACC+CGG - chr4_3:58007689-58007708 None:intergenic 50.0%
TGGTGAGGTGCATGACACAA+TGG + chr4_3:58007815-58007834 Msa0650760:three_prime_UTR 50.0%
TTATCCCAGTATGTCCAGGG+AGG + chr4_3:58007927-58007946 Msa0650760:three_prime_UTR 50.0%
GTAGAAACACTCCTGGACTC+TGG - chr4_3:58008020-58008039 None:intergenic 50.0%
CGTGTGAGTAGAAACACTCC+TGG - chr4_3:58008027-58008046 None:intergenic 50.0%
GAGTTGTGGGGGTTACGGTT+AGG - chr4_3:58005015-58005034 None:intergenic 55.0%
ACCGTCACACTAAGGTGGTC+CGG + chr4_3:58005052-58005071 Msa0650760:CDS 55.0%
ACCGGACCACCTTAGTGTGA+CGG - chr4_3:58005056-58005075 None:intergenic 55.0%
GAAGTTGTCGTAGGAGGAGG+TGG - chr4_3:58005125-58005144 None:intergenic 55.0%
!! TGGAACGGAGGTTGATGAGG+GGG - chr4_3:58005210-58005229 None:intergenic 55.0%
! GGTTTCCGGTTTGTGGCAAG+GGG - chr4_3:58005303-58005322 None:intergenic 55.0%
! GCGAGAAGGTTTCCGGTTTG+TGG - chr4_3:58005310-58005329 None:intergenic 55.0%
CCTCTCCAGAAATGGCGAGA+AGG - chr4_3:58005324-58005343 None:intergenic 55.0%
! CCTTCTCGCCATTTCTGGAG+AGG + chr4_3:58005321-58005340 Msa0650760:CDS 55.0%
GTATGTGACGAGGGATCCGA+AGG + chr4_3:58005420-58005439 Msa0650760:CDS 55.0%
TCATATGGGAGGTCTTGCGG+TGG + chr4_3:58005474-58005493 Msa0650760:CDS 55.0%
! CGCGTGTGAAGAGGTATGAG+AGG + chr4_3:58005497-58005516 Msa0650760:CDS 55.0%
! GCTCCTGCTAACGAGAGTGT+TGG + chr4_3:58005535-58005554 Msa0650760:CDS 55.0%
!! AGTGTTGGTTGGAGGGATCC+AGG + chr4_3:58005550-58005569 Msa0650760:CDS 55.0%
ATCCTTTGTCCCCTGCTCTC+CGG - chr4_3:58007604-58007623 None:intergenic 55.0%
TGTATCGTGGGGGGTGAGTT+TGG + chr4_3:58007732-58007751 Msa0650760:exon 55.0%
GTATCGTGGGGGGTGAGTTT+GGG + chr4_3:58007733-58007752 Msa0650760:exon 55.0%
GGCTACAAAGCAGAAGCTCG+TGG + chr4_3:58007770-58007789 Msa0650760:three_prime_UTR 55.0%
GCTTCCTCCCTGGACATACT+GGG - chr4_3:58007934-58007953 None:intergenic 55.0%
! GAGGAAGCGTACTGGTGCTT+GGG + chr4_3:58007946-58007965 Msa0650760:three_prime_UTR 55.0%
! AGGAAGCGTACTGGTGCTTG+GGG + chr4_3:58007947-58007966 Msa0650760:three_prime_UTR 55.0%
ACTGGTGCTTGGGGCCTTTA+TGG + chr4_3:58007956-58007975 Msa0650760:three_prime_UTR 55.0%
CTGGTGCTTGGGGCCTTTAT+GGG + chr4_3:58007957-58007976 Msa0650760:three_prime_UTR 55.0%
GTGGGGGTTACGGTTAGGGA+GGG - chr4_3:58005010-58005029 None:intergenic 60.0%
TGTGGGGGTTACGGTTAGGG+AGG - chr4_3:58005011-58005030 None:intergenic 60.0%
CCTTAGTGTGACGGTGTCCC+CGG - chr4_3:58005047-58005066 None:intergenic 60.0%
GGGACACCGTCACACTAAGG+TGG + chr4_3:58005047-58005066 Msa0650760:CDS 60.0%
TTGTCGTAGGAGGAGGTGGG+TGG - chr4_3:58005121-58005140 None:intergenic 60.0%
TCCACCTGGCAATCCGGTTC+CGG + chr4_3:58005172-58005191 Msa0650760:CDS 60.0%
!! ACGGAGGTTGATGAGGGGGA+GGG - chr4_3:58005206-58005225 None:intergenic 60.0%
!! AACGGAGGTTGATGAGGGGG+AGG - chr4_3:58005207-58005226 None:intergenic 60.0%
GAACATGGCCCCAAAGGGCA+AGG - chr4_3:58007526-58007545 None:intergenic 60.0%
CTTGTGGAAATGGCGTGGGC+CGG + chr4_3:58007582-58007601 Msa0650760:CDS 60.0%
CGCTTCCTCCCTGGACATAC+TGG - chr4_3:58007935-58007954 None:intergenic 60.0%
! TGTCCAGGGAGGAAGCGTAC+TGG + chr4_3:58007938-58007957 Msa0650760:three_prime_UTR 60.0%
! GGAGGAAGCGTACTGGTGCT+TGG + chr4_3:58007945-58007964 Msa0650760:three_prime_UTR 60.0%
CCGGGGACACCGTCACACTA+AGG + chr4_3:58005044-58005063 Msa0650760:CDS 65.0%
TGTGCCGGAACCGGATTGCC+AGG - chr4_3:58005179-58005198 None:intergenic 65.0%
! GAGGGAGAGTGTGCCGGAAC+CGG - chr4_3:58005188-58005207 None:intergenic 65.0%
TTGCGGTGGCGCGTGTGAAG+AGG + chr4_3:58005488-58005507 Msa0650760:CDS 65.0%
GCACCAGTACGCTTCCTCCC+TGG - chr4_3:58007944-58007963 None:intergenic 65.0%
GCCGGAACCGGATTGCCAGG+TGG - chr4_3:58005176-58005195 None:intergenic 70.0%
! GAGGGGGAGGGAGAGTGTGC+CGG - chr4_3:58005194-58005213 None:intergenic 70.0%
AATGGCGTGGGCCGGAGAGC+AGG + chr4_3:58007590-58007609 Msa0650760:CDS 70.0%
ATGGCGTGGGCCGGAGAGCA+GGG + chr4_3:58007591-58007610 Msa0650760:CDS 70.0%
GGCCGGAGAGCAGGGGACAA+AGG + chr4_3:58007599-58007618 Msa0650760:CDS 70.0%
TGGCGTGGGCCGGAGAGCAG+GGG + chr4_3:58007592-58007611 Msa0650760:CDS 75.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 58004938 58008079 58004938 ID=Msa0650760;Name=Msa0650760
chr4_3 mRNA 58004938 58008079 58004938 ID=Msa0650760-mRNA-1;Parent=Msa0650760;Name=Msa0650760-mRNA-1;_AED=0.28;_eAED=0.28;_QI=0|1|0.5|1|1|1|2|331|548
chr4_3 exon 58004938 58005630 58004938 ID=Msa0650760-mRNA-1:exon:15269;Parent=Msa0650760-mRNA-1
chr4_3 exon 58006795 58008079 58006795 ID=Msa0650760-mRNA-1:exon:15270;Parent=Msa0650760-mRNA-1
chr4_3 CDS 58004938 58005630 58004938 ID=Msa0650760-mRNA-1:cds;Parent=Msa0650760-mRNA-1
chr4_3 CDS 58006795 58007748 58006795 ID=Msa0650760-mRNA-1:cds;Parent=Msa0650760-mRNA-1
chr4_3 three_prime_UTR 58007749 58008079 58007749 ID=Msa0650760-mRNA-1:three_prime_utr;Parent=Msa0650760-mRNA-1
Gene Sequence

>Msa0650760

ATGATTCCTTGCACAATACTCTTCCTCTATCTTTCAATAATCTTCAACTCCAACCTTGTCCAATCAAAACCCTCCCTAACCGTAACCCCCACAACTCTATCAAAATCCGGGGACACCGTCACACTAAGGTGGTCCGGTATCCAATCACCCTCAGAGCTAGACTTCGTAGCAATCTACTCACCACCCACCTCCTCCTACGACAACTTCATTGGCTACCTCTTCCTCTCTAAATCATCCACCTGGCAATCCGGTTCCGGCACACTCTCCCTCCCCCTCATCAACCTCCGTTCCAACTACATCTTCGGTATCTTCCATTGGACCCAATCCGAAATCAACCCTGATCGTTATGACCATGACAATAACCCCTTGCCACAAACCGGAAACCTTCTCGCCATTTCTGGAGAGGTTTCGTTTGTTTCGGGTAAAGGACCGGAGCAGATTCATCTTGCTTTTGCGGATGAAGAAGATGCTATGCGGGTTATGTATGTGACGAGGGATCCGAAGGAGACGTATGTGAGGTATGGGGAGAGAAAATGTCATATGGGAGGTCTTGCGGTGGCGCGTGTGAAGAGGTATGAGAGGGAGCATATGTGTGATGCTCCTGCTAACGAGAGTGTTGGTTGGAGGGATCCAGGTTATATACATGATGCTTTGATCACTAGTTTAAAGAAAGGAAGAAGATACTACTATAAGGTTGGAAATAAAAATGGAGGTTGGAGTGCAACTCATAGCTTTGTGTCAAGGAATAGTGATTCAAATGAAACAATAGCTTTCATTTTTGGTGACATGGGAACAGCTACACCATATAATACATTTTTGCGTACACAAGAAGAAAGCATATCAACAGTGAAGTGGATCCTCCGTGATGTTGAAGCTCTAGGAAACAAACCCGCTTTTGTGTCACACATCGGAGACATTAGTTACGCAAGTGGTTATGCGTGGTTGTGGGATCATTTTTTCGCACAAATTGAACCTGTTGCAACAAAAGTGGCATACCATGTATGCATTGGAAATCATGAGTATGATTGGCCTTTACAGCCATGGAAACCTAATTGGACTAATTATGGAAAAGATGGAGGTGGTGAGTGCGGTGTACCCTATAGTTTAAGGTTCAACATGCCTGGAAATTCGTCAGAACCTACTGGAACTATAGCTCCAGCAACTAGAAATCTTTATTACTCATTTGATATGGGTGTAGTACATTTTGTTTATATATCAACAGAGACCAATTTTCTTCTTGGGAGCAACCAGTATAACTTCTTGAAGCATGATTTGGAATCAGTTGACAGGAACAAGACTCCTTTTGTAGTAGTTCAAGGGCACAGACCCATGTACACAACAATCAATGGAACCAAGGATGTTGCGTTGAGAGAAAAGATGCTTGAGCACTTGGAACCTCTATTGGTGAACAACAATGTGAACCTTGCCCTTTGGGGCCATGTTCATAGATACGAGAGATTTTGTCCACTGAATAACTACACTTGTGGAAATGGCGTGGGCCGGAGAGCAGGGGACAAAGGATATACCGTTCATCTTGTGATTGGCATGGCAGGGCAAGACAAGCAATCCATCTGGAAAACAAGACCGGGTCATCCCAATGACTCGATCTTTCCTCAACCAAAACAGTCTTTGTATCGTGGGGGGTGA
Protein sequence

>Msa0650760

MIPCTILFLYLSIIFNSNLVQSKPSLTVTPTTLSKSGDTVTLRWSGIQSPSELDFVAIYSPPTSSYDNFIGYLFLSKSSTWQSGSGTLSLPLINLRSNYIFGIFHWTQSEINPDRYDHDNNPLPQTGNLLAISGEVSFVSGKGPEQIHLAFADEEDAMRVMYVTRDPKETYVRYGERKCHMGGLAVARVKRYEREHMCDAPANESVGWRDPGYIHDALITSLKKGRRYYYKVGNKNGGWSATHSFVSRNSDSNETIAFIFGDMGTATPYNTFLRTQEESISTVKWILRDVEALGNKPAFVSHIGDISYASGYAWLWDHFFAQIEPVATKVAYHVCIGNHEYDWPLQPWKPNWTNYGKDGGGECGVPYSLRFNMPGNSSEPTGTIAPATRNLYYSFDMGVVHFVYISTETNFLLGSNQYNFLKHDLESVDRNKTPFVVVQGHRPMYTTINGTKDVALREKMLEHLEPLLVNNNVNLALWGHVHRYERFCPLNNYTCGNGVGRRAGDKGYTVHLVIGMAGQDKQSIWKTRPGHPNDSIFPQPKQSLYRGG*