AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0650670

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0650670 MtrunA17_Chr4g0043001 94.343 548 31 0 1 548 1 548 0.0 1083
Msa0650670 MtrunA17_Chr4g0043021 83.789 549 87 2 1 548 1 548 0.0 936
Msa0650670 MtrunA17_Chr4g0022251 36.062 452 228 16 85 518 173 581 1.43e-68 233
Msa0650670 MtrunA17_Chr1g0173511 33.118 465 236 16 85 518 36 456 3.87e-64 217
Msa0650670 MtrunA17_Chr8g0364481 32.210 534 281 21 25 518 46 538 7.85e-64 219
Msa0650670 MtrunA17_Chr5g0419621 34.279 458 236 18 83 518 131 545 1.40e-61 213
Msa0650670 MtrunA17_Chr4g0057551 30.755 556 293 21 20 529 55 564 8.90e-58 203
Msa0650670 MtrunA17_Chr3g0116051 29.541 545 294 20 20 518 49 549 7.63e-57 201
Msa0650670 MtrunA17_Chr3g0116091 27.273 407 214 15 128 518 5 345 4.42e-36 139
Msa0650670 MtrunA17_Chr7g0266151 27.456 397 220 11 144 537 47 378 1.52e-31 126
Msa0650670 MtrunA17_Chr7g0266131 30.649 385 202 17 118 496 30 355 2.94e-31 126
Msa0650670 MtrunA17_Chr5g0443881 28.818 406 211 16 144 546 49 379 1.46e-28 118
Msa0650670 MtrunA17_Chr1g0178831 28.045 353 190 12 140 487 42 335 2.37e-26 111
Msa0650670 MtrunA17_Chr3g0098721 27.586 406 216 15 144 546 43 373 3.15e-26 111
Msa0650670 MtrunA17_Chr3g0121411 28.000 425 216 16 139 519 66 444 4.39e-24 105
Msa0650670 MtrunA17_Chr3g0121431 27.934 426 215 17 139 519 121 499 1.60e-23 104
Msa0650670 MtrunA17_Chr7g0236041 25.386 453 238 17 139 548 65 460 4.16e-21 97.1
Msa0650670 MtrunA17_Chr4g0019091 25.243 412 246 12 143 546 54 411 6.92e-21 95.9
Msa0650670 MtrunA17_Chr3g0116071 32.558 172 91 7 192 343 48 214 7.73e-20 89.4
Msa0650670 MtrunA17_Chr6g0453731 26.266 316 163 9 212 496 146 422 3.25e-19 91.3
Msa0650670 MtrunA17_Chr4g0064541 24.408 422 236 16 143 546 54 410 1.45e-18 88.6
Msa0650670 MtrunA17_Chr1g0211041 24.943 441 250 16 125 546 35 413 6.98e-18 86.7
Msa0650670 MtrunA17_Chr4g0019071 25.564 266 164 6 287 546 51 288 3.11e-13 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0650670 AT1G13900.1 63.553 546 188 4 6 548 8 545 0.0 745
Msa0650670 AT2G03450.1 63.570 549 185 6 5 548 7 545 0.0 726
Msa0650670 AT1G13750.1 33.462 520 256 21 20 495 42 515 1.92e-66 227
Msa0650670 AT5G50400.1 32.353 510 282 18 44 529 78 548 1.56e-63 219
Msa0650670 AT5G50400.2 32.985 479 263 17 70 529 37 476 6.89e-63 215
Msa0650670 AT4G24890.1 29.616 547 306 21 11 518 35 541 4.48e-57 201
Msa0650670 AT3G52820.1 30.548 383 195 14 140 518 43 358 2.69e-32 129
Msa0650670 AT3G52780.1 30.556 396 212 13 144 537 44 378 1.48e-31 127
Msa0650670 AT3G52780.2 31.412 347 184 12 144 488 44 338 2.57e-31 125
Msa0650670 AT3G52810.1 30.618 356 179 13 144 492 51 345 3.68e-28 117
Msa0650670 AT3G20500.1 26.500 400 226 12 140 537 43 376 8.91e-26 110
Msa0650670 AT4G13700.1 29.716 387 213 14 139 496 60 416 2.41e-25 109
Msa0650670 AT3G07130.1 30.409 342 160 13 212 524 140 432 1.52e-21 98.6
Msa0650670 AT4G13700.2 28.726 369 207 13 139 481 60 398 6.06e-21 95.9
Msa0650670 AT2G27190.1 27.841 352 184 15 212 546 118 416 1.03e-19 92.4
Msa0650670 AT2G32770.3 26.817 399 214 16 144 496 69 435 9.02e-19 90.1
Msa0650670 AT3G07130.2 29.582 311 151 11 212 494 140 410 1.78e-18 89.4
Msa0650670 AT2G32770.2 28.803 309 174 11 212 496 68 354 3.33e-18 87.8
Msa0650670 AT4G36350.1 28.116 345 191 13 212 546 114 411 1.00e-17 86.3
Msa0650670 AT1G56360.1 26.979 341 200 10 212 546 114 411 1.61e-17 85.9
Msa0650670 AT5G34850.1 24.819 415 243 16 143 546 53 409 1.06e-16 83.2
Msa0650670 AT2G18130.1 25.882 255 145 7 303 546 171 392 8.09e-15 77.4
Msa0650670 AT2G32770.1 26.087 391 198 18 144 496 69 406 8.44e-11 65.1

Find 199 sgRNAs with CRISPR-Local

Find 293 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTGGTGCTTGGGGCCTTTA+TGG 0.124406 4_3:+57896233 Msa0650670:three_prime_UTR
GGAAACCTTCTCGCCATTTC+TGG 0.134192 4_3:+57893593 Msa0650670:CDS
GCATGACACAATGGAAATTT+TGG 0.186747 4_3:+57896101 Msa0650670:three_prime_UTR
TCTTTATTACTCATTTGATA+TGG 0.193459 4_3:+57895548 Msa0650670:CDS
TACAGCCATGGAAACCTAAT+TGG 0.237477 4_3:+57895412 Msa0650670:CDS
AAACCTAATTGGACTAATTA+TGG 0.245800 4_3:+57895423 Msa0650670:CDS
CAATGTGAACCTTGCCCTTT+GGG 0.254495 4_3:+57895791 Msa0650670:CDS
GCCTTTATGGGTTACATTCT+CGG 0.263370 4_3:+57896246 Msa0650670:three_prime_UTR
GGAGAGGTTTCGTTTGTTTC+GGG 0.266915 4_3:+57893614 Msa0650670:CDS
TGGAGAGGTTTCGTTTGTTT+CGG 0.270883 4_3:+57893613 Msa0650670:CDS
TAACTTCTTGAAGCATGATT+TGG 0.286113 4_3:+57895632 Msa0650670:CDS
CGCTTCCTCCCTGGACATAC+TGG 0.298280 4_3:-57896209 None:intergenic
GAGGAAGCGTACTGGTGCTT+GGG 0.309443 4_3:+57896223 Msa0650670:three_prime_UTR
TTTCCATAATTAGTCCAATT+AGG 0.311578 4_3:-57895426 None:intergenic
CTGGTGCTTGGGGCCTTTAT+GGG 0.316117 4_3:+57896234 Msa0650670:three_prime_UTR
AGAAATGGCGAGAAGGTTTC+CGG 0.335298 4_3:-57893591 None:intergenic
CCACGATACAAAGACTGTTT+TGG 0.338175 4_3:-57895996 None:intergenic
GGTAATGTTAATGTTGATAT+TGG 0.343693 4_3:+57896144 Msa0650670:three_prime_UTR
GCTTCTTCAATCAAACCTTT+AGG 0.347032 4_3:-57896177 None:intergenic
AATAACTACACTTGTGGAAA+TGG 0.347897 4_3:+57895849 Msa0650670:CDS
ATCAGGGTTGATTTCGGATT+GGG 0.356573 4_3:-57893534 None:intergenic
GCGGTGTACCCTATAGTTTA+AGG 0.356975 4_3:+57895466 Msa0650670:CDS
TGAGCACTTGGAACCTCTAT+TGG 0.358489 4_3:+57895761 Msa0650670:CDS
TCCACCTGGCAATCCGGTTC+CGG 0.362173 4_3:+57893449 Msa0650670:CDS
ACAATGTGAACCTTGCCCTT+TGG 0.362789 4_3:+57895790 Msa0650670:CDS
TAATAAAGATTTCTAGTTGC+TGG 0.363307 4_3:-57895534 None:intergenic
GGAGGAAGCGTACTGGTGCT+TGG 0.371645 4_3:+57896222 Msa0650670:three_prime_UTR
GTAGGTTCTGACGAATTTCC+AGG 0.373251 4_3:-57895498 None:intergenic
TGAAGATTATTGAAAGATAG+AGG 0.374624 4_3:-57893238 None:intergenic
TCCATTGATTGTTGTGTACA+TGG 0.378076 4_3:-57895706 None:intergenic
AATGGCGTGGGCCGGAGAGC+AGG 0.380124 4_3:+57895867 Msa0650670:CDS
CATGCTTCAAGAAGTTATAC+TGG 0.380553 4_3:-57895626 None:intergenic
ATAACGATCAGGGTTGATTT+CGG 0.381601 4_3:-57893540 None:intergenic
CTTTATTACTCATTTGATAT+GGG 0.382012 4_3:+57895549 Msa0650670:CDS
GTTGCTCCCAAGAAGAAAAT+TGG 0.386841 4_3:-57895604 None:intergenic
GAGGGAGAGTGTGCCGGAAC+CGG 0.388793 4_3:-57893462 None:intergenic
TGGGGAGAGAAAATGTCATA+TGG 0.392406 4_3:+57893736 Msa0650670:CDS
AGGAAGAAGATACTACTATA+AGG 0.392651 4_3:+57893886 Msa0650670:CDS
GTTTCGTTTGTTTCGGGTAA+AGG 0.396676 4_3:+57893620 Msa0650670:CDS
CATCTTGTGATTGGCATGGC+AGG 0.399832 4_3:+57895909 Msa0650670:CDS
CATGTTGAACCTTAAACTAT+AGG 0.406709 4_3:-57895475 None:intergenic
CCCACAACTCTATCAAAATC+CGG 0.407569 4_3:+57893302 Msa0650670:CDS
TTGAGGAAAGATCGAGTCAT+TGG 0.409792 4_3:-57895973 None:intergenic
TTCTCTCAACGCAACATCCT+TGG 0.412679 4_3:-57895730 None:intergenic
TTGTCATGGTCATAACGATC+AGG 0.414015 4_3:-57893551 None:intergenic
TATACCGTTCATCTTGTGAT+TGG 0.415416 4_3:+57895900 Msa0650670:CDS
AGAAAAGATGCTTGAGCACT+TGG 0.415912 4_3:+57895749 Msa0650670:CDS
TGGACTAATTATGGAAAAGA+TGG 0.416929 4_3:+57895432 Msa0650670:CDS
GCGAGAAGGTTTCCGGTTTG+TGG 0.419688 4_3:-57893584 None:intergenic
TGGATGATTTAGAGAGGAAG+AGG 0.425118 4_3:-57893430 None:intergenic
ACCGAGAATGTAACCCATAA+AGG 0.427471 4_3:-57896247 None:intergenic
AGTCTAGCTCTGAGGGTGAT+TGG 0.427544 4_3:-57893355 None:intergenic
AAACGAAACCTCTCCAGAAA+TGG 0.431051 4_3:-57893606 None:intergenic
GAAGTTGTCGTAGGAGGAGG+TGG 0.439677 4_3:-57893399 None:intergenic
TGTGCCGGAACCGGATTGCC+AGG 0.440070 4_3:-57893453 None:intergenic
TCCTCCTACGACAACTTCAT+TGG 0.441288 4_3:+57893404 Msa0650670:CDS
TATGGGTTACATTCTCGGTT+GGG 0.441433 4_3:+57896251 Msa0650670:three_prime_UTR
ATTGGTAATGCTAAACCTAA+AGG 0.441714 4_3:+57896162 Msa0650670:three_prime_UTR
ATGTTGAACCTTAAACTATA+GGG 0.442633 4_3:-57895474 None:intergenic
AAGCTCGTGGTTTCTTATGT+TGG 0.444757 4_3:+57896060 Msa0650670:three_prime_UTR
TTAGGTTTCCATGGCTGTAA+AGG 0.446726 4_3:-57895408 None:intergenic
CTCCGTTCCAACTACATCTT+CGG 0.446774 4_3:+57893497 Msa0650670:CDS
AGGTTTCCGGTTTGTGGCAA+GGG 0.449186 4_3:-57893578 None:intergenic
TTATGGGTTACATTCTCGGT+TGG 0.451487 4_3:+57896250 Msa0650670:three_prime_UTR
ACATTGTTGTTCACCAATAG+AGG 0.452089 4_3:-57895774 None:intergenic
TTGATCACTAGTTTAAAGAA+AGG 0.454168 4_3:+57893866 Msa0650670:CDS
AGTGTTGGTTGGAGGGATCC+AGG 0.454568 4_3:+57893827 Msa0650670:CDS
ATGGCGTGGGCCGGAGAGCA+GGG 0.457953 4_3:+57895868 Msa0650670:CDS
TGCCCTTGAACTACTACAAA+AGG 0.467528 4_3:-57895678 None:intergenic
AGTTTAAGGTTCAACATGCC+TGG 0.468330 4_3:+57895480 Msa0650670:CDS
AAGGAGACGTATGTGAGGTA+TGG 0.469112 4_3:+57893716 Msa0650670:CDS
AATAACCCCTTGCCACAAAC+CGG 0.470128 4_3:+57893572 Msa0650670:CDS
CCTTAGTGTGACGGTGTCCC+CGG 0.471605 4_3:-57893321 None:intergenic
CCCATGTACACAACAATCAA+TGG 0.475166 4_3:+57895705 Msa0650670:CDS
CATGATTTCCAATGCATACA+TGG 0.476850 4_3:-57895374 None:intergenic
CAACCAACACTCTCGTTAGC+AGG 0.479587 4_3:-57893815 None:intergenic
TACATTATCCCAGTATGTCC+AGG 0.482507 4_3:+57896200 Msa0650670:three_prime_UTR
GTGGCAAGGGGTTATTGTCA+TGG 0.483320 4_3:-57893565 None:intergenic
GGCAAGACAAGCAATCCATC+TGG 0.484465 4_3:+57895931 Msa0650670:CDS
GTTGGAACGGAGGTTGATGA+GGG 0.484503 4_3:-57893486 None:intergenic
AAATCATCCACCTGGCAATC+CGG 0.487160 4_3:+57893443 Msa0650670:CDS
TTGGAAATCATGAGTATGAT+TGG 0.491026 4_3:+57895385 Msa0650670:CDS
TACCTCACATACGTCTCCTT+CGG 0.495897 4_3:-57893713 None:intergenic
AAGGTTTCCGGTTTGTGGCA+AGG 0.496203 4_3:-57893579 None:intergenic
AGGAGACGTATGTGAGGTAT+GGG 0.496851 4_3:+57893717 Msa0650670:CDS
ACATCTTCGGTATCTTCCAT+TGG 0.497800 4_3:+57893510 Msa0650670:CDS
TGAGGAAAGATCGAGTCATT+GGG 0.499179 4_3:-57895972 None:intergenic
TTAGTCCAATTAGGTTTCCA+TGG 0.501745 4_3:-57895417 None:intergenic
ATCTATGAACATGGCCCCAA+AGG 0.501753 4_3:-57895806 None:intergenic
GATCAGGGTTGATTTCGGAT+TGG 0.501872 4_3:-57893535 None:intergenic
GTGGCATACCATGTATGCAT+TGG 0.503990 4_3:+57895366 Msa0650670:CDS
ATCCATCTGGAAAACAAGAC+CGG 0.506758 4_3:+57895944 Msa0650670:CDS
ATCCTTTGTCCCCTGCTCTC+CGG 0.507689 4_3:-57895878 None:intergenic
GCACCAGTACGCTTCCTCCC+TGG 0.509555 4_3:-57896218 None:intergenic
TTGCGGTGGCGCGTGTGAAG+AGG 0.510510 4_3:+57893765 Msa0650670:CDS
CTCTGAGGGTGATTGGATAC+CGG 0.512218 4_3:-57893348 None:intergenic
GGAAATTCGTCAGAACCTAC+TGG 0.514003 4_3:+57895501 Msa0650670:CDS
CAAGTGGTTATGCGTGGTTG+TGG 0.514084 4_3:+57895304 Msa0650670:CDS
CCTTCTCGCCATTTCTGGAG+AGG 0.514435 4_3:+57893598 Msa0650670:CDS
GGGGAGAGAAAATGTCATAT+GGG 0.518872 4_3:+57893737 Msa0650670:CDS
ACCGTCACACTAAGGTGGTC+CGG 0.520460 4_3:+57893329 Msa0650670:CDS
TCTATGAACATGGCCCCAAA+GGG 0.523434 4_3:-57895805 None:intergenic
CTAACGAGAGTGTTGGTTGG+AGG 0.524469 4_3:+57893819 Msa0650670:CDS
CCACAACTCTATCAAAATCC+GGG 0.525087 4_3:+57893303 Msa0650670:CDS
GTTTGTTTCGGGTAAAGGAC+CGG 0.527184 4_3:+57893625 Msa0650670:CDS
ATGATTTGGAATCAGTTGAC+AGG 0.528504 4_3:+57895646 Msa0650670:CDS
TGAACCTGTTGCAACAAAAG+TGG 0.530909 4_3:+57895347 Msa0650670:CDS
AAGTGGTTATGCGTGGTTGT+GGG 0.532637 4_3:+57895305 Msa0650670:CDS
TCTTATGTTGGAAATCATGA+TGG 0.533637 4_3:+57896072 Msa0650670:three_prime_UTR
AGTTGGAACGGAGGTTGATG+AGG 0.534951 4_3:-57893487 None:intergenic
CAAAACAGTCTTTGTATCGT+GGG 0.537627 4_3:+57895997 Msa0650670:CDS
AATGAAGTTGTCGTAGGAGG+AGG 0.537994 4_3:-57893402 None:intergenic
GAAGATACCGAAGATGTAGT+TGG 0.538252 4_3:-57893504 None:intergenic
TGGCGTGGGCCGGAGAGCAG+GGG 0.538311 4_3:+57895869 Msa0650670:CDS
ATGATTGGCCTTTACAGCCA+TGG 0.542059 4_3:+57895400 Msa0650670:CDS
CTCCGTGATGTTGAAGCTCT+AGG 0.548924 4_3:+57895237 Msa0650670:CDS
GGCCGGAGAGCAGGGGACAA+AGG 0.549021 4_3:+57895876 Msa0650670:CDS
CCATTGATTGTTGTGTACAT+GGG 0.550156 4_3:-57895705 None:intergenic
ATCTTGTGATTGGCATGGCA+GGG 0.555722 4_3:+57895910 Msa0650670:CDS
TGATTTCGGATTGGGTCCAA+TGG 0.556134 4_3:-57893526 None:intergenic
AAAGCATATCAACAGTGAAG+TGG 0.556245 4_3:+57895211 Msa0650670:CDS
GCCAATGAAGTTGTCGTAGG+AGG 0.560684 4_3:-57893405 None:intergenic
AAAGCAAGATGAATCTGCTC+CGG 0.560908 4_3:-57893644 None:intergenic
CTGCTAACGAGAGTGTTGGT+TGG 0.561825 4_3:+57893816 Msa0650670:CDS
TAACGAGAGTGTTGGTTGGA+GGG 0.568916 4_3:+57893820 Msa0650670:CDS
CCACAAGTGTAGTTATTCAG+TGG 0.569232 4_3:-57895843 None:intergenic
CCGGGGACACCGTCACACTA+AGG 0.569874 4_3:+57893321 Msa0650670:CDS
ATCGAGTCATTGGGATGACC+CGG 0.570194 4_3:-57895963 None:intergenic
TGTCCAGGGAGGAAGCGTAC+TGG 0.570210 4_3:+57896215 Msa0650670:three_prime_UTR
ATCTCTCGTATCTATGAACA+TGG 0.572154 4_3:-57895815 None:intergenic
CCTCTCCAGAAATGGCGAGA+AGG 0.575084 4_3:-57893598 None:intergenic
TGTCATGGTCATAACGATCA+GGG 0.575204 4_3:-57893550 None:intergenic
CAACTCATAGCTTTGTGTCA+AGG 0.575696 4_3:+57895100 Msa0650670:CDS
CCACTGAATAACTACACTTG+TGG 0.577944 4_3:+57895843 Msa0650670:CDS
CTTGTGGAAATGGCGTGGGC+CGG 0.578247 4_3:+57895859 Msa0650670:CDS
TCGGTTGGGTTATACATGCA+AGG 0.580242 4_3:+57896265 Msa0650670:three_prime_UTR
TTCCTAGAGCTTCAACATCA+CGG 0.580505 4_3:-57895239 None:intergenic
CGTTCATCTTGTGATTGGCA+TGG 0.581543 4_3:+57895905 Msa0650670:CDS
ATGTCATATGGGAGGTCTTG+CGG 0.583227 4_3:+57893748 Msa0650670:CDS
TAGAGGAAGAGTATTGTGCA+AGG 0.584858 4_3:-57893221 None:intergenic
GAACATGGCCCCAAAGGGCA+AGG 0.584999 4_3:-57895800 None:intergenic
GGATGAAGAAGATGCTATGC+GGG 0.585911 4_3:+57893670 Msa0650670:CDS
GTAGAAACACTCCTGGACTC+TGG 0.586121 4_3:-57896294 None:intergenic
GCTTCCTCCCTGGACATACT+GGG 0.586632 4_3:-57896208 None:intergenic
ACTAATTATGGAAAAGATGG+AGG 0.587578 4_3:+57895435 Msa0650670:CDS
TACACTTGTGGAAATGGCGT+GGG 0.587976 4_3:+57895855 Msa0650670:CDS
CCAAAACAGTCTTTGTATCG+TGG 0.588649 4_3:+57895996 Msa0650670:CDS
GCTACGAAGTCTAGCTCTGA+GGG 0.589676 4_3:-57893362 None:intergenic
CCGATGTGTGACACAAAAGC+GGG 0.592792 4_3:-57895267 None:intergenic
TACCGAAGATGTAGTTGGAA+CGG 0.595020 4_3:-57893499 None:intergenic
AATTATGGAAAAGATGGAGG+TGG 0.595238 4_3:+57895438 Msa0650670:CDS
CTACACTTGTGGAAATGGCG+TGG 0.595264 4_3:+57895854 Msa0650670:CDS
AAAGCATCATGTATATAACC+TGG 0.595486 4_3:-57893845 None:intergenic
TCCATCTGGAAAACAAGACC+GGG 0.597952 4_3:+57895945 Msa0650670:CDS
GGTTTCCGGTTTGTGGCAAG+GGG 0.598797 4_3:-57893577 None:intergenic
CGTGTGAGTAGAAACACTCC+TGG 0.600020 4_3:-57896301 None:intergenic
AGGAAGCGTACTGGTGCTTG+GGG 0.600088 4_3:+57896224 Msa0650670:three_prime_UTR
GTATGTGACGAGGGATCCGA+AGG 0.606007 4_3:+57893697 Msa0650670:CDS
CACAACAATCAATGGAACCA+AGG 0.611304 4_3:+57895713 Msa0650670:CDS
TCCTCTCTAAATCATCCACC+TGG 0.615171 4_3:+57893435 Msa0650670:CDS
TCCGATGTGTGACACAAAAG+CGG 0.615605 4_3:-57895268 None:intergenic
CGCGTGTGAAGAGGTATGAG+AGG 0.617971 4_3:+57893774 Msa0650670:CDS
AAAACAGTCTTTGTATCGTG+GGG 0.618337 4_3:+57895998 Msa0650670:CDS
TGGAACGGAGGTTGATGAGG+GGG 0.618753 4_3:-57893484 None:intergenic
GTTTGGGTACATTAGACTAG+TGG 0.624624 4_3:+57896026 Msa0650670:three_prime_UTR
GTAGCCAATGAAGTTGTCGT+AGG 0.625292 4_3:-57893408 None:intergenic
ACCGGACCACCTTAGTGTGA+CGG 0.627721 4_3:-57893330 None:intergenic
TTGTCGTAGGAGGAGGTGGG+TGG 0.632479 4_3:-57893395 None:intergenic
AAAGATGGAGGTGGTGAGTG+CGG 0.636261 4_3:+57895447 Msa0650670:CDS
GCCAGGTGGATGATTTAGAG+AGG 0.636449 4_3:-57893436 None:intergenic
GCTGGAGCTATAGTTCCAGT+AGG 0.636710 4_3:-57895516 None:intergenic
ACATTATCCCAGTATGTCCA+GGG 0.637529 4_3:+57896201 Msa0650670:three_prime_UTR
AAGTTGTCGTAGGAGGAGGT+GGG 0.638967 4_3:-57893398 None:intergenic
GCGGGTTATGTATGTGACGA+GGG 0.644902 4_3:+57893688 Msa0650670:CDS
TTGGAACGGAGGTTGATGAG+GGG 0.645080 4_3:-57893485 None:intergenic
GGAGACATTAGTTACGCAAG+TGG 0.646032 4_3:+57895288 Msa0650670:CDS
AAACAGTCTTTGTATCGTGG+GGG 0.648873 4_3:+57895999 Msa0650670:CDS
AATGTGAACCTTGCCCTTTG+GGG 0.650716 4_3:+57895792 Msa0650670:CDS
TGCGGGTTATGTATGTGACG+AGG 0.651449 4_3:+57893687 Msa0650670:CDS
GGGACACCGTCACACTAAGG+TGG 0.653790 4_3:+57893324 Msa0650670:CDS
AACAGTCTTTGTATCGTGGG+GGG 0.656017 4_3:+57896000 Msa0650670:CDS
TGCTACGAAGTCTAGCTCTG+AGG 0.656528 4_3:-57893363 None:intergenic
GTTACGCAAGTGGTTATGCG+TGG 0.658494 4_3:+57895298 Msa0650670:CDS
CATGCCAATCACAAGATGAA+CGG 0.659360 4_3:-57895904 None:intergenic
GCGTGTGAAGAGGTATGAGA+GGG 0.660850 4_3:+57893775 Msa0650670:CDS
GGCTACAAAGCAGAAGCTCG+TGG 0.661109 4_3:+57896047 Msa0650670:three_prime_UTR
GCTCCTGCTAACGAGAGTGT+TGG 0.663854 4_3:+57893812 Msa0650670:CDS
CACAACTCTATCAAAATCCG+GGG 0.669749 4_3:+57893304 Msa0650670:CDS
CGGATGAAGAAGATGCTATG+CGG 0.672413 4_3:+57893669 Msa0650670:CDS
ATCCGAAGGAGACGTATGTG+AGG 0.673765 4_3:+57893711 Msa0650670:CDS
GAGAGAAAATGTCATATGGG+AGG 0.677448 4_3:+57893740 Msa0650670:CDS
CGAAGATGTAGTTGGAACGG+AGG 0.680198 4_3:-57893496 None:intergenic
TCATATGGGAGGTCTTGCGG+TGG 0.686577 4_3:+57893751 Msa0650670:CDS
TGTTGGAAATCATGATGGTG+AGG 0.687796 4_3:+57896077 Msa0650670:three_prime_UTR
GCCGGAACCGGATTGCCAGG+TGG 0.689201 4_3:-57893450 None:intergenic
GGAGACGTATGTGAGGTATG+GGG 0.695565 4_3:+57893718 Msa0650670:CDS
TGGTGAGGTGCATGACACAA+TGG 0.697263 4_3:+57896092 Msa0650670:three_prime_UTR
CTAGAGCTTCAACATCACGG+AGG 0.777257 4_3:-57895236 None:intergenic
TTATCCCAGTATGTCCAGGG+AGG 0.778942 4_3:+57896204 Msa0650670:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTATTTTTTAATAATA+AGG + chr4_3:57894837-57894856 Msa0650670:intron 0.0%
!! AATAATGAAAGTAAAAAACA+AGG - chr4_3:57893986-57894005 None:intergenic 15.0%
!!! TTTTGCTTAATTTTATTGAA+TGG + chr4_3:57894606-57894625 Msa0650670:intron 15.0%
!!! TTTGCTTAATTTTATTGAAT+GGG + chr4_3:57894607-57894626 Msa0650670:intron 15.0%
!! AAAATTATGATAGAAAACAA+AGG + chr4_3:57894897-57894916 Msa0650670:intron 15.0%
!!! AAAACAAAATCATTGTTTTA+AGG + chr4_3:57894960-57894979 Msa0650670:intron 15.0%
!! TTTCATTATTACTTTGTGTT+TGG + chr4_3:57893996-57894015 Msa0650670:intron 20.0%
!!! AAAATACCTCGATATTTTTT+TGG + chr4_3:57894237-57894256 Msa0650670:intron 20.0%
!!! AAATACCTCGATATTTTTTT+GGG + chr4_3:57894238-57894257 Msa0650670:intron 20.0%
!!! TGGAAAAGATTTTTAAGTAT+TGG + chr4_3:57894690-57894709 Msa0650670:intron 20.0%
!! TTGTATATCCAAATATAGTT+GGG - chr4_3:57894750-57894769 None:intergenic 20.0%
!! ATTGTATATCCAAATATAGT+TGG - chr4_3:57894751-57894770 None:intergenic 20.0%
!! TTGTATATCCAAATATAGTT+GGG - chr4_3:57894750-57894769 None:intergenic 20.0%
!! ATTGTATATCCAAATATAGT+TGG - chr4_3:57894751-57894770 None:intergenic 20.0%
!! TCTTTATTACTCATTTGATA+TGG + chr4_3:57895548-57895567 Msa0650670:CDS 20.0%
!! CTTTATTACTCATTTGATAT+GGG + chr4_3:57895549-57895568 Msa0650670:CDS 20.0%
! TGAAGATTATTGAAAGATAG+AGG - chr4_3:57893241-57893260 None:intergenic 25.0%
!! TTGATCACTAGTTTAAAGAA+AGG + chr4_3:57893866-57893885 Msa0650670:CDS 25.0%
! ATGAAAGTAAAAAACAAGGT+AGG - chr4_3:57893982-57894001 None:intergenic 25.0%
! ATTATTACTTTGTGTTTGGT+TGG + chr4_3:57894000-57894019 Msa0650670:intron 25.0%
!! GGTAATGGTTTAAAGTTTAA+AGG + chr4_3:57894072-57894091 Msa0650670:intron 25.0%
!!! TTTGATTTGATAAAGAGACA+AGG + chr4_3:57894266-57894285 Msa0650670:intron 25.0%
! CACTAAATCATAAGAAAACT+TGG + chr4_3:57894390-57894409 Msa0650670:intron 25.0%
!!! ATTTTTGATAGTTTGCGTTA+TGG - chr4_3:57894500-57894519 None:intergenic 25.0%
!!! AAGATTTTTAAGTATTGGTC+TGG + chr4_3:57894695-57894714 Msa0650670:intron 25.0%
! TCATGTAATCGTTTAGTATA+TGG + chr4_3:57895009-57895028 Msa0650670:intron 25.0%
!! GAAACAATAGCTTTCATTTT+TGG + chr4_3:57895138-57895157 Msa0650670:CDS 25.0%
! GTACGCAAAAATGTATTATA+TGG - chr4_3:57895183-57895202 None:intergenic 25.0%
! AAACCTAATTGGACTAATTA+TGG + chr4_3:57895423-57895442 Msa0650670:CDS 25.0%
! TTTCCATAATTAGTCCAATT+AGG - chr4_3:57895429-57895448 None:intergenic 25.0%
! ATGTTGAACCTTAAACTATA+GGG - chr4_3:57895477-57895496 None:intergenic 25.0%
!!! TAATAAAGATTTCTAGTTGC+TGG - chr4_3:57895537-57895556 None:intergenic 25.0%
! GGTAATGTTAATGTTGATAT+TGG + chr4_3:57896144-57896163 Msa0650670:three_prime_UTR 25.0%
! AAAGCATCATGTATATAACC+TGG - chr4_3:57893848-57893867 None:intergenic 30.0%
AGGAAGAAGATACTACTATA+AGG + chr4_3:57893886-57893905 Msa0650670:CDS 30.0%
CAAGGTAATCAAAATTAGAC+CGG + chr4_3:57894027-57894046 Msa0650670:intron 30.0%
! CATTAAACATGTGTTGGTAA+TGG + chr4_3:57894051-57894070 Msa0650670:intron 30.0%
GGAGAACTAAAATCGTATTT+CGG - chr4_3:57894524-57894543 None:intergenic 30.0%
!! ATATAGTTGGGTTCTAAACA+TGG - chr4_3:57894790-57894809 None:intergenic 30.0%
! GTTTAGAACCCAACTATATT+TGG + chr4_3:57894791-57894810 Msa0650670:intron 30.0%
GATTACATGAACCAGATTTA+GGG - chr4_3:57894999-57895018 None:intergenic 30.0%
! TACCAGGTTGGAAATAAAAA+TGG + chr4_3:57895066-57895085 Msa0650670:intron 30.0%
TTGGAAATAAAAATGGAGGT+TGG + chr4_3:57895073-57895092 Msa0650670:CDS 30.0%
TTGGAAATCATGAGTATGAT+TGG + chr4_3:57895385-57895404 Msa0650670:CDS 30.0%
TGGACTAATTATGGAAAAGA+TGG + chr4_3:57895432-57895451 Msa0650670:CDS 30.0%
ACTAATTATGGAAAAGATGG+AGG + chr4_3:57895435-57895454 Msa0650670:CDS 30.0%
CATGTTGAACCTTAAACTAT+AGG - chr4_3:57895478-57895497 None:intergenic 30.0%
TAACTTCTTGAAGCATGATT+TGG + chr4_3:57895632-57895651 Msa0650670:CDS 30.0%
AATAACTACACTTGTGGAAA+TGG + chr4_3:57895849-57895868 Msa0650670:CDS 30.0%
TCTTATGTTGGAAATCATGA+TGG + chr4_3:57896072-57896091 Msa0650670:three_prime_UTR 30.0%
!! ATTGGTAATGCTAAACCTAA+AGG + chr4_3:57896162-57896181 Msa0650670:three_prime_UTR 30.0%
! ATAACGATCAGGGTTGATTT+CGG - chr4_3:57893543-57893562 None:intergenic 35.0%
TTGTGTTTGGTTGGAAAACA+AGG + chr4_3:57894009-57894028 Msa0650670:intron 35.0%
ACCAACACATGTTTAATGTC+CGG - chr4_3:57894049-57894068 None:intergenic 35.0%
!! ACATGTGTTGGTAATGGTAA+TGG + chr4_3:57894057-57894076 Msa0650670:intron 35.0%
ACACACCCAAAAAAATATCG+AGG - chr4_3:57894246-57894265 None:intergenic 35.0%
! ACCCTGTTTGATTTACAAAC+TGG + chr4_3:57894309-57894328 Msa0650670:intron 35.0%
GCCAGTTTGTAAATCAAACA+GGG - chr4_3:57894313-57894332 None:intergenic 35.0%
TTGATTTACAAACTGGCCAT+TGG + chr4_3:57894316-57894335 Msa0650670:intron 35.0%
!!! ACTTGTTTTGTCTAGTCCAA+TGG - chr4_3:57894335-57894354 None:intergenic 35.0%
TCATAAGAAAACTTGGCCAT+TGG + chr4_3:57894397-57894416 Msa0650670:intron 35.0%
!!! ACTTGTTTTGTCTAGTCCAA+TGG - chr4_3:57894335-57894354 None:intergenic 35.0%
CCAAATATAGTTGGGTTCTA+CGG - chr4_3:57894742-57894761 None:intergenic 35.0%
CGATTACATGAACCAGATTT+AGG - chr4_3:57895000-57895019 None:intergenic 35.0%
CATCACTCAAGTTAAGTTTC+TGG - chr4_3:57895038-57895057 None:intergenic 35.0%
! CAGAAACTTAACTTGAGTGA+TGG + chr4_3:57895036-57895055 Msa0650670:intron 35.0%
!! CTCCATTTTTATTTCCAACC+TGG - chr4_3:57895071-57895090 None:intergenic 35.0%
! CAGGTTGGAAATAAAAATGG+AGG + chr4_3:57895069-57895088 Msa0650670:intron 35.0%
!!! AGCTTTCATTTTTGGTGACA+TGG + chr4_3:57895146-57895165 Msa0650670:CDS 35.0%
!!! GCTTTCATTTTTGGTGACAT+GGG + chr4_3:57895147-57895166 Msa0650670:CDS 35.0%
AAAGCATATCAACAGTGAAG+TGG + chr4_3:57895211-57895230 Msa0650670:CDS 35.0%
CATGATTTCCAATGCATACA+TGG - chr4_3:57895377-57895396 None:intergenic 35.0%
TTAGTCCAATTAGGTTTCCA+TGG - chr4_3:57895420-57895439 None:intergenic 35.0%
AATTATGGAAAAGATGGAGG+TGG + chr4_3:57895438-57895457 Msa0650670:CDS 35.0%
! ACAGAGACCAATTTTCTTCT+TGG + chr4_3:57895597-57895616 Msa0650670:CDS 35.0%
! CAGAGACCAATTTTCTTCTT+GGG + chr4_3:57895598-57895617 Msa0650670:CDS 35.0%
CATGCTTCAAGAAGTTATAC+TGG - chr4_3:57895629-57895648 None:intergenic 35.0%
! ATGATTTGGAATCAGTTGAC+AGG + chr4_3:57895646-57895665 Msa0650670:CDS 35.0%
! ACTCCTTTTGTAGTAGTTCA+AGG + chr4_3:57895675-57895694 Msa0650670:CDS 35.0%
! CTCCTTTTGTAGTAGTTCAA+GGG + chr4_3:57895676-57895695 Msa0650670:CDS 35.0%
! CCATTGATTGTTGTGTACAT+GGG - chr4_3:57895708-57895727 None:intergenic 35.0%
! TCCATTGATTGTTGTGTACA+TGG - chr4_3:57895709-57895728 None:intergenic 35.0%
! ACATTGTTGTTCACCAATAG+AGG - chr4_3:57895777-57895796 None:intergenic 35.0%
ATCTCTCGTATCTATGAACA+TGG - chr4_3:57895818-57895837 None:intergenic 35.0%
TATACCGTTCATCTTGTGAT+TGG + chr4_3:57895900-57895919 Msa0650670:CDS 35.0%
!!! TACAAAGACTGTTTTGGTTG+AGG - chr4_3:57895993-57896012 None:intergenic 35.0%
CAAAACAGTCTTTGTATCGT+GGG + chr4_3:57895997-57896016 Msa0650670:CDS 35.0%
AAAACAGTCTTTGTATCGTG+GGG + chr4_3:57895998-57896017 Msa0650670:CDS 35.0%
! GCATGACACAATGGAAATTT+TGG + chr4_3:57896101-57896120 Msa0650670:three_prime_UTR 35.0%
!! ACAATGGAAATTTTGGCATC+TGG + chr4_3:57896108-57896127 Msa0650670:three_prime_UTR 35.0%
GCTTCTTCAATCAAACCTTT+AGG - chr4_3:57896180-57896199 None:intergenic 35.0%
!! TAGAGGAAGAGTATTGTGCA+AGG - chr4_3:57893224-57893243 None:intergenic 40.0%
!! GGATTTTGATAGAGTTGTGG+GGG - chr4_3:57893303-57893322 None:intergenic 40.0%
!! CGGATTTTGATAGAGTTGTG+GGG - chr4_3:57893304-57893323 None:intergenic 40.0%
!! CCGGATTTTGATAGAGTTGT+GGG - chr4_3:57893305-57893324 None:intergenic 40.0%
CCCACAACTCTATCAAAATC+CGG + chr4_3:57893302-57893321 Msa0650670:CDS 40.0%
CCACAACTCTATCAAAATCC+GGG + chr4_3:57893303-57893322 Msa0650670:CDS 40.0%
CACAACTCTATCAAAATCCG+GGG + chr4_3:57893304-57893323 Msa0650670:CDS 40.0%
TGGATGATTTAGAGAGGAAG+AGG - chr4_3:57893433-57893452 None:intergenic 40.0%
TACCGAAGATGTAGTTGGAA+CGG - chr4_3:57893502-57893521 None:intergenic 40.0%
GAAGATACCGAAGATGTAGT+TGG - chr4_3:57893507-57893526 None:intergenic 40.0%
ACATCTTCGGTATCTTCCAT+TGG + chr4_3:57893510-57893529 Msa0650670:CDS 40.0%
! ATCAGGGTTGATTTCGGATT+GGG - chr4_3:57893537-57893556 None:intergenic 40.0%
TGTCATGGTCATAACGATCA+GGG - chr4_3:57893553-57893572 None:intergenic 40.0%
TTGTCATGGTCATAACGATC+AGG - chr4_3:57893554-57893573 None:intergenic 40.0%
AAACGAAACCTCTCCAGAAA+TGG - chr4_3:57893609-57893628 None:intergenic 40.0%
TGGAGAGGTTTCGTTTGTTT+CGG + chr4_3:57893613-57893632 Msa0650670:CDS 40.0%
! GTTTCGTTTGTTTCGGGTAA+AGG + chr4_3:57893620-57893639 Msa0650670:CDS 40.0%
AAAGCAAGATGAATCTGCTC+CGG - chr4_3:57893647-57893666 None:intergenic 40.0%
!! GCAGATTCATCTTGCTTTTG+CGG + chr4_3:57893649-57893668 Msa0650670:CDS 40.0%
TGGGGAGAGAAAATGTCATA+TGG + chr4_3:57893736-57893755 Msa0650670:CDS 40.0%
GGGGAGAGAAAATGTCATAT+GGG + chr4_3:57893737-57893756 Msa0650670:CDS 40.0%
GAGAGAAAATGTCATATGGG+AGG + chr4_3:57893740-57893759 Msa0650670:CDS 40.0%
ACCGGACATTAAACATGTGT+TGG + chr4_3:57894045-57894064 Msa0650670:intron 40.0%
GGCCAGTTTGTAAATCAAAC+AGG - chr4_3:57894314-57894333 None:intergenic 40.0%
ATCATCGTCTAGTGAGAAAG+AGG + chr4_3:57894631-57894650 Msa0650670:intron 40.0%
TGAGAAAGAGGATCATGAGT+TGG + chr4_3:57894643-57894662 Msa0650670:intron 40.0%
GAGAAAGAGGATCATGAGTT+GGG + chr4_3:57894644-57894663 Msa0650670:intron 40.0%
!! TCATCGATTGCTGTTTGAGT+TGG + chr4_3:57894670-57894689 Msa0650670:intron 40.0%
CCGTAGAACCCAACTATATT+TGG + chr4_3:57894739-57894758 Msa0650670:intron 40.0%
AAACAAACACGCCCTAAATC+TGG + chr4_3:57894985-57895004 Msa0650670:intron 40.0%
!! CAACTCATAGCTTTGTGTCA+AGG + chr4_3:57895100-57895119 Msa0650670:CDS 40.0%
TTCCTAGAGCTTCAACATCA+CGG - chr4_3:57895242-57895261 None:intergenic 40.0%
TGAACCTGTTGCAACAAAAG+TGG + chr4_3:57895347-57895366 Msa0650670:CDS 40.0%
!!! TATGCCACTTTTGTTGCAAC+AGG - chr4_3:57895354-57895373 None:intergenic 40.0%
TTAGGTTTCCATGGCTGTAA+AGG - chr4_3:57895411-57895430 None:intergenic 40.0%
TACAGCCATGGAAACCTAAT+TGG + chr4_3:57895412-57895431 Msa0650670:CDS 40.0%
! AGTTTAAGGTTCAACATGCC+TGG + chr4_3:57895480-57895499 Msa0650670:CDS 40.0%
GTTGCTCCCAAGAAGAAAAT+TGG - chr4_3:57895607-57895626 None:intergenic 40.0%
TGCCCTTGAACTACTACAAA+AGG - chr4_3:57895681-57895700 None:intergenic 40.0%
CCCATGTACACAACAATCAA+TGG + chr4_3:57895705-57895724 Msa0650670:CDS 40.0%
CACAACAATCAATGGAACCA+AGG + chr4_3:57895713-57895732 Msa0650670:CDS 40.0%
AGAAAAGATGCTTGAGCACT+TGG + chr4_3:57895749-57895768 Msa0650670:CDS 40.0%
CCACAAGTGTAGTTATTCAG+TGG - chr4_3:57895846-57895865 None:intergenic 40.0%
CCACTGAATAACTACACTTG+TGG + chr4_3:57895843-57895862 Msa0650670:CDS 40.0%
CATGCCAATCACAAGATGAA+CGG - chr4_3:57895907-57895926 None:intergenic 40.0%
ATCCATCTGGAAAACAAGAC+CGG + chr4_3:57895944-57895963 Msa0650670:CDS 40.0%
TGAGGAAAGATCGAGTCATT+GGG - chr4_3:57895975-57895994 None:intergenic 40.0%
TTGAGGAAAGATCGAGTCAT+TGG - chr4_3:57895976-57895995 None:intergenic 40.0%
! CCACGATACAAAGACTGTTT+TGG - chr4_3:57895999-57896018 None:intergenic 40.0%
CCAAAACAGTCTTTGTATCG+TGG + chr4_3:57895996-57896015 Msa0650670:CDS 40.0%
AAACAGTCTTTGTATCGTGG+GGG + chr4_3:57895999-57896018 Msa0650670:CDS 40.0%
GTTTGGGTACATTAGACTAG+TGG + chr4_3:57896026-57896045 Msa0650670:three_prime_UTR 40.0%
AAGCTCGTGGTTTCTTATGT+TGG + chr4_3:57896060-57896079 Msa0650670:three_prime_UTR 40.0%
! TGTTGGAAATCATGATGGTG+AGG + chr4_3:57896077-57896096 Msa0650670:three_prime_UTR 40.0%
! GCATCTGGAGAACTTTTCAA+TGG + chr4_3:57896123-57896142 Msa0650670:three_prime_UTR 40.0%
TACATTATCCCAGTATGTCC+AGG + chr4_3:57896200-57896219 Msa0650670:three_prime_UTR 40.0%
ACATTATCCCAGTATGTCCA+GGG + chr4_3:57896201-57896220 Msa0650670:three_prime_UTR 40.0%
! GCCTTTATGGGTTACATTCT+CGG + chr4_3:57896246-57896265 Msa0650670:three_prime_UTR 40.0%
ACCGAGAATGTAACCCATAA+AGG - chr4_3:57896250-57896269 None:intergenic 40.0%
! TTATGGGTTACATTCTCGGT+TGG + chr4_3:57896250-57896269 Msa0650670:three_prime_UTR 40.0%
! TATGGGTTACATTCTCGGTT+GGG + chr4_3:57896251-57896270 Msa0650670:three_prime_UTR 40.0%
!!! GGGTTTTGATTGGACAAGGT+TGG - chr4_3:57893267-57893286 None:intergenic 45.0%
TGATAGAGTTGTGGGGGTTA+CGG - chr4_3:57893297-57893316 None:intergenic 45.0%
!! CCCGGATTTTGATAGAGTTG+TGG - chr4_3:57893306-57893325 None:intergenic 45.0%
AATGAAGTTGTCGTAGGAGG+AGG - chr4_3:57893405-57893424 None:intergenic 45.0%
TCCTCCTACGACAACTTCAT+TGG + chr4_3:57893404-57893423 Msa0650670:CDS 45.0%
GTAGCCAATGAAGTTGTCGT+AGG - chr4_3:57893411-57893430 None:intergenic 45.0%
TCCTCTCTAAATCATCCACC+TGG + chr4_3:57893435-57893454 Msa0650670:CDS 45.0%
AAATCATCCACCTGGCAATC+CGG + chr4_3:57893443-57893462 Msa0650670:CDS 45.0%
CTCCGTTCCAACTACATCTT+CGG + chr4_3:57893497-57893516 Msa0650670:CDS 45.0%
! TGATTTCGGATTGGGTCCAA+TGG - chr4_3:57893529-57893548 None:intergenic 45.0%
! GATCAGGGTTGATTTCGGAT+TGG - chr4_3:57893538-57893557 None:intergenic 45.0%
AATAACCCCTTGCCACAAAC+CGG + chr4_3:57893572-57893591 Msa0650670:CDS 45.0%
AGAAATGGCGAGAAGGTTTC+CGG - chr4_3:57893594-57893613 None:intergenic 45.0%
GGAGAGGTTTCGTTTGTTTC+GGG + chr4_3:57893614-57893633 Msa0650670:CDS 45.0%
! GTTTGTTTCGGGTAAAGGAC+CGG + chr4_3:57893625-57893644 Msa0650670:CDS 45.0%
CGGATGAAGAAGATGCTATG+CGG + chr4_3:57893669-57893688 Msa0650670:CDS 45.0%
GGATGAAGAAGATGCTATGC+GGG + chr4_3:57893670-57893689 Msa0650670:CDS 45.0%
TACCTCACATACGTCTCCTT+CGG - chr4_3:57893716-57893735 None:intergenic 45.0%
AAGGAGACGTATGTGAGGTA+TGG + chr4_3:57893716-57893735 Msa0650670:CDS 45.0%
AGGAGACGTATGTGAGGTAT+GGG + chr4_3:57893717-57893736 Msa0650670:CDS 45.0%
ATGTCATATGGGAGGTCTTG+CGG + chr4_3:57893748-57893767 Msa0650670:CDS 45.0%
!! TAACGAGAGTGTTGGTTGGA+GGG + chr4_3:57893820-57893839 Msa0650670:CDS 45.0%
! TACGGTTGACTAGCTGACAA+GGG - chr4_3:57894724-57894743 None:intergenic 45.0%
TCCGATGTGTGACACAAAAG+CGG - chr4_3:57895271-57895290 None:intergenic 45.0%
GGAGACATTAGTTACGCAAG+TGG + chr4_3:57895288-57895307 Msa0650670:CDS 45.0%
AAGTGGTTATGCGTGGTTGT+GGG + chr4_3:57895305-57895324 Msa0650670:CDS 45.0%
GTGGCATACCATGTATGCAT+TGG + chr4_3:57895366-57895385 Msa0650670:CDS 45.0%
!! ATGATTGGCCTTTACAGCCA+TGG + chr4_3:57895400-57895419 Msa0650670:CDS 45.0%
GCGGTGTACCCTATAGTTTA+AGG + chr4_3:57895466-57895485 Msa0650670:CDS 45.0%
! GTAGGTTCTGACGAATTTCC+AGG - chr4_3:57895501-57895520 None:intergenic 45.0%
GGAAATTCGTCAGAACCTAC+TGG + chr4_3:57895501-57895520 Msa0650670:CDS 45.0%
TTCTCTCAACGCAACATCCT+TGG - chr4_3:57895733-57895752 None:intergenic 45.0%
TGAGCACTTGGAACCTCTAT+TGG + chr4_3:57895761-57895780 Msa0650670:CDS 45.0%
ACAATGTGAACCTTGCCCTT+TGG + chr4_3:57895790-57895809 Msa0650670:CDS 45.0%
CAATGTGAACCTTGCCCTTT+GGG + chr4_3:57895791-57895810 Msa0650670:CDS 45.0%
AATGTGAACCTTGCCCTTTG+GGG + chr4_3:57895792-57895811 Msa0650670:CDS 45.0%
TCTATGAACATGGCCCCAAA+GGG - chr4_3:57895808-57895827 None:intergenic 45.0%
ATCTATGAACATGGCCCCAA+AGG - chr4_3:57895809-57895828 None:intergenic 45.0%
TACACTTGTGGAAATGGCGT+GGG + chr4_3:57895855-57895874 Msa0650670:CDS 45.0%
CGTTCATCTTGTGATTGGCA+TGG + chr4_3:57895905-57895924 Msa0650670:CDS 45.0%
ATCTTGTGATTGGCATGGCA+GGG + chr4_3:57895910-57895929 Msa0650670:CDS 45.0%
TCCATCTGGAAAACAAGACC+GGG + chr4_3:57895945-57895964 Msa0650670:CDS 45.0%
AACAGTCTTTGTATCGTGGG+GGG + chr4_3:57896000-57896019 Msa0650670:CDS 45.0%
TCGGTTGGGTTATACATGCA+AGG + chr4_3:57896265-57896284 Msa0650670:three_prime_UTR 45.0%
CAAGGAAAAAACCAGAGTCC+AGG + chr4_3:57896283-57896302 Msa0650670:three_prime_UTR 45.0%
!!! ATATACATTTTAAAAATTAT+AGG - chr4_3:57894100-57894119 None:intergenic 5.0%
!! ATAAAAAAAAAATATATTTG+AGG + chr4_3:57894188-57894207 Msa0650670:intron 5.0%
!!! ATTTATAATATTTTTTATGT+TGG - chr4_3:57894545-57894564 None:intergenic 5.0%
!!! GGGAGGGTTTTGATTGGACA+AGG - chr4_3:57893271-57893290 None:intergenic 50.0%
!! CGGTTAGGGAGGGTTTTGAT+TGG - chr4_3:57893277-57893296 None:intergenic 50.0%
AGTTGTGGGGGTTACGGTTA+GGG - chr4_3:57893291-57893310 None:intergenic 50.0%
! CTCTGAGGGTGATTGGATAC+CGG - chr4_3:57893351-57893370 None:intergenic 50.0%
!! AGTCTAGCTCTGAGGGTGAT+TGG - chr4_3:57893358-57893377 None:intergenic 50.0%
!! GCTACGAAGTCTAGCTCTGA+GGG - chr4_3:57893365-57893384 None:intergenic 50.0%
!! TGCTACGAAGTCTAGCTCTG+AGG - chr4_3:57893366-57893385 None:intergenic 50.0%
AAGTTGTCGTAGGAGGAGGT+GGG - chr4_3:57893401-57893420 None:intergenic 50.0%
GCCAATGAAGTTGTCGTAGG+AGG - chr4_3:57893408-57893427 None:intergenic 50.0%
GCCAGGTGGATGATTTAGAG+AGG - chr4_3:57893439-57893458 None:intergenic 50.0%
!! TTGGAACGGAGGTTGATGAG+GGG - chr4_3:57893488-57893507 None:intergenic 50.0%
!! GTTGGAACGGAGGTTGATGA+GGG - chr4_3:57893489-57893508 None:intergenic 50.0%
!! AGTTGGAACGGAGGTTGATG+AGG - chr4_3:57893490-57893509 None:intergenic 50.0%
CGAAGATGTAGTTGGAACGG+AGG - chr4_3:57893499-57893518 None:intergenic 50.0%
GTGGCAAGGGGTTATTGTCA+TGG - chr4_3:57893568-57893587 None:intergenic 50.0%
! AGGTTTCCGGTTTGTGGCAA+GGG - chr4_3:57893581-57893600 None:intergenic 50.0%
! AAGGTTTCCGGTTTGTGGCA+AGG - chr4_3:57893582-57893601 None:intergenic 50.0%
GGAAACCTTCTCGCCATTTC+TGG + chr4_3:57893593-57893612 Msa0650670:CDS 50.0%
! TGCGGGTTATGTATGTGACG+AGG + chr4_3:57893687-57893706 Msa0650670:CDS 50.0%
! GCGGGTTATGTATGTGACGA+GGG + chr4_3:57893688-57893707 Msa0650670:CDS 50.0%
ATCCGAAGGAGACGTATGTG+AGG + chr4_3:57893711-57893730 Msa0650670:CDS 50.0%
GGAGACGTATGTGAGGTATG+GGG + chr4_3:57893718-57893737 Msa0650670:CDS 50.0%
! GCGTGTGAAGAGGTATGAGA+GGG + chr4_3:57893775-57893794 Msa0650670:CDS 50.0%
CAACCAACACTCTCGTTAGC+AGG - chr4_3:57893818-57893837 None:intergenic 50.0%
!! CTGCTAACGAGAGTGTTGGT+TGG + chr4_3:57893816-57893835 Msa0650670:CDS 50.0%
!! CTAACGAGAGTGTTGGTTGG+AGG + chr4_3:57893819-57893838 Msa0650670:CDS 50.0%
! CTACGGTTGACTAGCTGACA+AGG - chr4_3:57894725-57894744 None:intergenic 50.0%
!! GAGTGATGGTGTTGTGTACC+AGG + chr4_3:57895050-57895069 Msa0650670:intron 50.0%
!! GATGGTGTTGTGTACCAGGT+TGG + chr4_3:57895054-57895073 Msa0650670:intron 50.0%
CTAGAGCTTCAACATCACGG+AGG - chr4_3:57895239-57895258 None:intergenic 50.0%
CTCCGTGATGTTGAAGCTCT+AGG + chr4_3:57895237-57895256 Msa0650670:CDS 50.0%
CCGATGTGTGACACAAAAGC+GGG - chr4_3:57895270-57895289 None:intergenic 50.0%
!! CCCGCTTTTGTGTCACACAT+CGG + chr4_3:57895267-57895286 Msa0650670:CDS 50.0%
GTTACGCAAGTGGTTATGCG+TGG + chr4_3:57895298-57895317 Msa0650670:CDS 50.0%
CAAGTGGTTATGCGTGGTTG+TGG + chr4_3:57895304-57895323 Msa0650670:CDS 50.0%
AAAGATGGAGGTGGTGAGTG+CGG + chr4_3:57895447-57895466 Msa0650670:CDS 50.0%
GCTGGAGCTATAGTTCCAGT+AGG - chr4_3:57895519-57895538 None:intergenic 50.0%
CTACACTTGTGGAAATGGCG+TGG + chr4_3:57895854-57895873 Msa0650670:CDS 50.0%
CATCTTGTGATTGGCATGGC+AGG + chr4_3:57895909-57895928 Msa0650670:CDS 50.0%
GGCAAGACAAGCAATCCATC+TGG + chr4_3:57895931-57895950 Msa0650670:CDS 50.0%
!!! ACCCGGTCTTGTTTTCCAGA+TGG - chr4_3:57895949-57895968 None:intergenic 50.0%
ATCGAGTCATTGGGATGACC+CGG - chr4_3:57895966-57895985 None:intergenic 50.0%
TGGTGAGGTGCATGACACAA+TGG + chr4_3:57896092-57896111 Msa0650670:three_prime_UTR 50.0%
TTATCCCAGTATGTCCAGGG+AGG + chr4_3:57896204-57896223 Msa0650670:three_prime_UTR 50.0%
GTAGAAACACTCCTGGACTC+TGG - chr4_3:57896297-57896316 None:intergenic 50.0%
CGTGTGAGTAGAAACACTCC+TGG - chr4_3:57896304-57896323 None:intergenic 50.0%
GAGTTGTGGGGGTTACGGTT+AGG - chr4_3:57893292-57893311 None:intergenic 55.0%
ACCGTCACACTAAGGTGGTC+CGG + chr4_3:57893329-57893348 Msa0650670:CDS 55.0%
ACCGGACCACCTTAGTGTGA+CGG - chr4_3:57893333-57893352 None:intergenic 55.0%
GAAGTTGTCGTAGGAGGAGG+TGG - chr4_3:57893402-57893421 None:intergenic 55.0%
!! TGGAACGGAGGTTGATGAGG+GGG - chr4_3:57893487-57893506 None:intergenic 55.0%
! GGTTTCCGGTTTGTGGCAAG+GGG - chr4_3:57893580-57893599 None:intergenic 55.0%
! GCGAGAAGGTTTCCGGTTTG+TGG - chr4_3:57893587-57893606 None:intergenic 55.0%
CCTCTCCAGAAATGGCGAGA+AGG - chr4_3:57893601-57893620 None:intergenic 55.0%
! CCTTCTCGCCATTTCTGGAG+AGG + chr4_3:57893598-57893617 Msa0650670:CDS 55.0%
GTATGTGACGAGGGATCCGA+AGG + chr4_3:57893697-57893716 Msa0650670:CDS 55.0%
TCATATGGGAGGTCTTGCGG+TGG + chr4_3:57893751-57893770 Msa0650670:CDS 55.0%
! CGCGTGTGAAGAGGTATGAG+AGG + chr4_3:57893774-57893793 Msa0650670:CDS 55.0%
! GCTCCTGCTAACGAGAGTGT+TGG + chr4_3:57893812-57893831 Msa0650670:CDS 55.0%
!! AGTGTTGGTTGGAGGGATCC+AGG + chr4_3:57893827-57893846 Msa0650670:CDS 55.0%
ATCCTTTGTCCCCTGCTCTC+CGG - chr4_3:57895881-57895900 None:intergenic 55.0%
TGTATCGTGGGGGGTGAGTT+TGG + chr4_3:57896009-57896028 Msa0650670:exon 55.0%
GTATCGTGGGGGGTGAGTTT+GGG + chr4_3:57896010-57896029 Msa0650670:exon 55.0%
GGCTACAAAGCAGAAGCTCG+TGG + chr4_3:57896047-57896066 Msa0650670:three_prime_UTR 55.0%
GCTTCCTCCCTGGACATACT+GGG - chr4_3:57896211-57896230 None:intergenic 55.0%
! GAGGAAGCGTACTGGTGCTT+GGG + chr4_3:57896223-57896242 Msa0650670:three_prime_UTR 55.0%
! AGGAAGCGTACTGGTGCTTG+GGG + chr4_3:57896224-57896243 Msa0650670:three_prime_UTR 55.0%
ACTGGTGCTTGGGGCCTTTA+TGG + chr4_3:57896233-57896252 Msa0650670:three_prime_UTR 55.0%
CTGGTGCTTGGGGCCTTTAT+GGG + chr4_3:57896234-57896253 Msa0650670:three_prime_UTR 55.0%
GTGGGGGTTACGGTTAGGGA+GGG - chr4_3:57893287-57893306 None:intergenic 60.0%
TGTGGGGGTTACGGTTAGGG+AGG - chr4_3:57893288-57893307 None:intergenic 60.0%
CCTTAGTGTGACGGTGTCCC+CGG - chr4_3:57893324-57893343 None:intergenic 60.0%
GGGACACCGTCACACTAAGG+TGG + chr4_3:57893324-57893343 Msa0650670:CDS 60.0%
TTGTCGTAGGAGGAGGTGGG+TGG - chr4_3:57893398-57893417 None:intergenic 60.0%
TCCACCTGGCAATCCGGTTC+CGG + chr4_3:57893449-57893468 Msa0650670:CDS 60.0%
!! ACGGAGGTTGATGAGGGGGA+GGG - chr4_3:57893483-57893502 None:intergenic 60.0%
!! AACGGAGGTTGATGAGGGGG+AGG - chr4_3:57893484-57893503 None:intergenic 60.0%
GAACATGGCCCCAAAGGGCA+AGG - chr4_3:57895803-57895822 None:intergenic 60.0%
CTTGTGGAAATGGCGTGGGC+CGG + chr4_3:57895859-57895878 Msa0650670:CDS 60.0%
CGCTTCCTCCCTGGACATAC+TGG - chr4_3:57896212-57896231 None:intergenic 60.0%
! TGTCCAGGGAGGAAGCGTAC+TGG + chr4_3:57896215-57896234 Msa0650670:three_prime_UTR 60.0%
! GGAGGAAGCGTACTGGTGCT+TGG + chr4_3:57896222-57896241 Msa0650670:three_prime_UTR 60.0%
CCGGGGACACCGTCACACTA+AGG + chr4_3:57893321-57893340 Msa0650670:CDS 65.0%
TGTGCCGGAACCGGATTGCC+AGG - chr4_3:57893456-57893475 None:intergenic 65.0%
! GAGGGAGAGTGTGCCGGAAC+CGG - chr4_3:57893465-57893484 None:intergenic 65.0%
TTGCGGTGGCGCGTGTGAAG+AGG + chr4_3:57893765-57893784 Msa0650670:CDS 65.0%
GCACCAGTACGCTTCCTCCC+TGG - chr4_3:57896221-57896240 None:intergenic 65.0%
GCCGGAACCGGATTGCCAGG+TGG - chr4_3:57893453-57893472 None:intergenic 70.0%
! GAGGGGGAGGGAGAGTGTGC+CGG - chr4_3:57893471-57893490 None:intergenic 70.0%
AATGGCGTGGGCCGGAGAGC+AGG + chr4_3:57895867-57895886 Msa0650670:CDS 70.0%
ATGGCGTGGGCCGGAGAGCA+GGG + chr4_3:57895868-57895887 Msa0650670:CDS 70.0%
GGCCGGAGAGCAGGGGACAA+AGG + chr4_3:57895876-57895895 Msa0650670:CDS 70.0%
TGGCGTGGGCCGGAGAGCAG+GGG + chr4_3:57895869-57895888 Msa0650670:CDS 75.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 57893215 57896356 57893215 ID=Msa0650670;Name=Msa0650670
chr4_3 mRNA 57893215 57896356 57893215 ID=Msa0650670-mRNA-1;Parent=Msa0650670;Name=Msa0650670-mRNA-1;_AED=0.28;_eAED=0.28;_QI=0|1|0.5|1|1|1|2|331|548
chr4_3 exon 57893215 57893907 57893215 ID=Msa0650670-mRNA-1:exon:15207;Parent=Msa0650670-mRNA-1
chr4_3 exon 57895072 57896356 57895072 ID=Msa0650670-mRNA-1:exon:15208;Parent=Msa0650670-mRNA-1
chr4_3 CDS 57893215 57893907 57893215 ID=Msa0650670-mRNA-1:cds;Parent=Msa0650670-mRNA-1
chr4_3 CDS 57895072 57896025 57895072 ID=Msa0650670-mRNA-1:cds;Parent=Msa0650670-mRNA-1
chr4_3 three_prime_UTR 57896026 57896356 57896026 ID=Msa0650670-mRNA-1:three_prime_utr;Parent=Msa0650670-mRNA-1
Gene Sequence

>Msa0650670

ATGATTCCTTGCACAATACTCTTCCTCTATCTTTCAATAATCTTCAACTCCAACCTTGTCCAATCAAAACCCTCCCTAACCGTAACCCCCACAACTCTATCAAAATCCGGGGACACCGTCACACTAAGGTGGTCCGGTATCCAATCACCCTCAGAGCTAGACTTCGTAGCAATCTACTCACCACCCACCTCCTCCTACGACAACTTCATTGGCTACCTCTTCCTCTCTAAATCATCCACCTGGCAATCCGGTTCCGGCACACTCTCCCTCCCCCTCATCAACCTCCGTTCCAACTACATCTTCGGTATCTTCCATTGGACCCAATCCGAAATCAACCCTGATCGTTATGACCATGACAATAACCCCTTGCCACAAACCGGAAACCTTCTCGCCATTTCTGGAGAGGTTTCGTTTGTTTCGGGTAAAGGACCGGAGCAGATTCATCTTGCTTTTGCGGATGAAGAAGATGCTATGCGGGTTATGTATGTGACGAGGGATCCGAAGGAGACGTATGTGAGGTATGGGGAGAGAAAATGTCATATGGGAGGTCTTGCGGTGGCGCGTGTGAAGAGGTATGAGAGGGAGCATATGTGTGATGCTCCTGCTAACGAGAGTGTTGGTTGGAGGGATCCAGGTTATATACATGATGCTTTGATCACTAGTTTAAAGAAAGGAAGAAGATACTACTATAAGGTTGGAAATAAAAATGGAGGTTGGAGTGCAACTCATAGCTTTGTGTCAAGGAATAGTGATTCAAATGAAACAATAGCTTTCATTTTTGGTGACATGGGAACAGCTACACCATATAATACATTTTTGCGTACACAAGAAGAAAGCATATCAACAGTGAAGTGGATCCTCCGTGATGTTGAAGCTCTAGGAAACAAACCCGCTTTTGTGTCACACATCGGAGACATTAGTTACGCAAGTGGTTATGCGTGGTTGTGGGATCATTTTTTCGCACAAATTGAACCTGTTGCAACAAAAGTGGCATACCATGTATGCATTGGAAATCATGAGTATGATTGGCCTTTACAGCCATGGAAACCTAATTGGACTAATTATGGAAAAGATGGAGGTGGTGAGTGCGGTGTACCCTATAGTTTAAGGTTCAACATGCCTGGAAATTCGTCAGAACCTACTGGAACTATAGCTCCAGCAACTAGAAATCTTTATTACTCATTTGATATGGGTGTAGTACATTTTGTTTATATATCAACAGAGACCAATTTTCTTCTTGGGAGCAACCAGTATAACTTCTTGAAGCATGATTTGGAATCAGTTGACAGGAACAAGACTCCTTTTGTAGTAGTTCAAGGGCACAGACCCATGTACACAACAATCAATGGAACCAAGGATGTTGCGTTGAGAGAAAAGATGCTTGAGCACTTGGAACCTCTATTGGTGAACAACAATGTGAACCTTGCCCTTTGGGGCCATGTTCATAGATACGAGAGATTTTGTCCACTGAATAACTACACTTGTGGAAATGGCGTGGGCCGGAGAGCAGGGGACAAAGGATATACCGTTCATCTTGTGATTGGCATGGCAGGGCAAGACAAGCAATCCATCTGGAAAACAAGACCGGGTCATCCCAATGACTCGATCTTTCCTCAACCAAAACAGTCTTTGTATCGTGGGGGGTGA
Protein sequence

>Msa0650670

MIPCTILFLYLSIIFNSNLVQSKPSLTVTPTTLSKSGDTVTLRWSGIQSPSELDFVAIYSPPTSSYDNFIGYLFLSKSSTWQSGSGTLSLPLINLRSNYIFGIFHWTQSEINPDRYDHDNNPLPQTGNLLAISGEVSFVSGKGPEQIHLAFADEEDAMRVMYVTRDPKETYVRYGERKCHMGGLAVARVKRYEREHMCDAPANESVGWRDPGYIHDALITSLKKGRRYYYKVGNKNGGWSATHSFVSRNSDSNETIAFIFGDMGTATPYNTFLRTQEESISTVKWILRDVEALGNKPAFVSHIGDISYASGYAWLWDHFFAQIEPVATKVAYHVCIGNHEYDWPLQPWKPNWTNYGKDGGGECGVPYSLRFNMPGNSSEPTGTIAPATRNLYYSFDMGVVHFVYISTETNFLLGSNQYNFLKHDLESVDRNKTPFVVVQGHRPMYTTINGTKDVALREKMLEHLEPLLVNNNVNLALWGHVHRYERFCPLNNYTCGNGVGRRAGDKGYTVHLVIGMAGQDKQSIWKTRPGHPNDSIFPQPKQSLYRGG*