AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0579310

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0579310 MtrunA17_Chr4g0043001 93.425 654 43 0 1 654 1 654 0.0 1277
Msa0579310 MtrunA17_Chr4g0043021 81.639 659 113 4 1 652 1 658 0.0 1062
Msa0579310 MtrunA17_Chr4g0022251 35.465 516 257 19 85 580 173 632 1.81e-73 248
Msa0579310 MtrunA17_Chr8g0364481 31.156 597 318 24 25 580 46 590 3.93e-68 234
Msa0579310 MtrunA17_Chr5g0419621 32.438 521 275 20 83 580 131 597 3.77e-66 228
Msa0579310 MtrunA17_Chr1g0173511 33.072 511 252 18 85 562 36 489 6.15e-66 224
Msa0579310 MtrunA17_Chr4g0057551 30.592 608 318 23 20 580 55 605 1.38e-63 221
Msa0579310 MtrunA17_Chr3g0116051 28.947 608 330 22 20 580 49 601 1.36e-62 219
Msa0579310 MtrunA17_Chr3g0116091 26.866 469 249 17 129 580 6 397 3.72e-41 155
Msa0579310 MtrunA17_Chr7g0266151 27.427 412 223 12 144 552 47 385 1.03e-32 131
Msa0579310 MtrunA17_Chr7g0266131 29.478 441 232 18 118 555 30 394 2.60e-31 127
Msa0579310 MtrunA17_Chr5g0443881 28.916 415 217 16 144 555 49 388 7.08e-30 123
Msa0579310 MtrunA17_Chr3g0098721 26.964 471 259 18 144 610 43 432 5.46e-28 117
Msa0579310 MtrunA17_Chr1g0178831 26.531 441 242 15 140 574 42 406 1.58e-27 115
Msa0579310 MtrunA17_Chr3g0121411 28.095 420 212 16 144 519 71 444 9.13e-24 105
Msa0579310 MtrunA17_Chr3g0121431 27.934 426 215 17 139 519 121 499 3.13e-23 104
Msa0579310 MtrunA17_Chr4g0019091 25.285 439 265 13 143 572 54 438 1.29e-22 101
Msa0579310 MtrunA17_Chr7g0236041 25.607 453 237 18 139 548 65 460 9.19e-21 96.7
Msa0579310 MtrunA17_Chr4g0064541 24.944 449 255 19 143 573 54 438 3.12e-20 94.4
Msa0579310 MtrunA17_Chr3g0116071 32.558 172 91 7 192 343 48 214 7.20e-20 89.7
Msa0579310 MtrunA17_Chr1g0211041 25.000 468 269 17 125 572 35 440 1.31e-19 92.4
Msa0579310 MtrunA17_Chr6g0453731 26.266 316 163 9 212 496 146 422 4.77e-19 91.3
Msa0579310 MtrunA17_Chr4g0019071 24.458 323 204 8 287 602 51 340 7.43e-16 79.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0579310 AT1G13900.1 60.809 643 232 6 6 637 8 641 0.0 827
Msa0579310 AT2G03450.1 61.742 643 225 9 5 641 7 634 0.0 813
Msa0579310 AT1G13750.1 32.401 608 306 25 20 580 42 591 3.97e-71 242
Msa0579310 AT5G50400.1 31.616 563 310 20 44 581 78 590 1.03e-68 236
Msa0579310 AT5G50400.2 32.143 532 291 19 70 581 37 518 3.48e-68 232
Msa0579310 AT4G24890.1 30.688 567 309 23 44 580 81 593 4.05e-62 218
Msa0579310 AT3G52780.1 30.529 416 218 14 144 557 44 390 2.10e-33 133
Msa0579310 AT3G52820.1 30.000 420 215 15 140 555 43 387 1.27e-32 131
Msa0579310 AT3G52780.2 31.412 347 184 12 144 488 44 338 2.42e-31 126
Msa0579310 AT3G52810.1 28.266 421 225 14 144 561 51 397 6.91e-29 120
Msa0579310 AT3G20500.1 26.068 468 262 17 140 603 43 430 3.05e-27 115
Msa0579310 AT4G13700.1 29.716 387 213 14 139 496 60 416 2.43e-25 110
Msa0579310 AT2G27190.1 27.704 379 203 16 212 572 118 443 8.02e-22 99.8
Msa0579310 AT3G07130.1 30.409 342 160 13 212 524 140 432 2.31e-21 98.6
Msa0579310 AT4G13700.2 28.726 369 207 13 139 481 60 398 4.92e-21 96.7
Msa0579310 AT2G32770.3 26.078 487 269 19 144 571 69 523 3.98e-20 94.7
Msa0579310 AT4G36350.1 26.817 399 234 14 212 599 114 465 9.62e-20 93.2
Msa0579310 AT2G32770.2 27.586 377 215 13 212 552 68 422 1.43e-19 92.4
Msa0579310 AT5G34850.1 24.078 461 280 17 143 591 53 455 2.75e-19 91.7
Msa0579310 AT1G56360.1 26.558 369 221 11 212 573 114 439 2.82e-19 91.7
Msa0579310 AT3G07130.2 29.582 311 151 11 212 494 140 410 2.54e-18 89.4
Msa0579310 AT2G18130.1 25.532 282 165 8 303 573 171 418 1.06e-16 83.6
Msa0579310 AT2G16430.2 24.270 445 262 15 143 572 58 442 3.62e-16 82.0
Msa0579310 AT2G16430.1 26.429 280 171 9 300 572 71 322 1.58e-14 75.9
Msa0579310 AT2G32770.1 25.470 479 253 21 144 571 69 494 2.93e-12 70.1
Msa0579310 AT3G46120.1 29.319 191 115 6 375 555 164 344 2.38e-11 66.6

Find 197 sgRNAs with CRISPR-Local

Find 291 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTGGTGCTTGGGGCCTTTA+TGG 0.124406 4_1:-83976211 Msa0579310:CDS
GCATGACACAATGGAAATTT+TGG 0.186747 4_1:-83976343 Msa0579310:CDS
TCTTTATTACTCATTTGATA+TGG 0.193459 4_1:-83976895 Msa0579310:CDS
TACAGCCATGGAAACCTAAT+TGG 0.237477 4_1:-83977031 Msa0579310:CDS
AAACCTAATTGGACTAATTA+TGG 0.245800 4_1:-83977020 Msa0579310:CDS
CAATGTGAACCTTGCCCTTT+GGG 0.254495 4_1:-83976652 Msa0579310:CDS
GCCTTTATGGGTTACATTCT+CGG 0.263370 4_1:-83976198 Msa0579310:CDS
GAAGAGGTTTCGTTTGTTTC+GGG 0.264619 4_1:-83978829 Msa0579310:CDS
TGAAGAGGTTTCGTTTGTTT+CGG 0.278246 4_1:-83978830 Msa0579310:CDS
TAACTTCTTGAAGCATGATT+TGG 0.286113 4_1:-83976811 Msa0579310:CDS
CGCTTCCTCCCTGGACATAC+TGG 0.298280 4_1:+83976235 None:intergenic
GAGGAAGCGTACTGGTGCTT+GGG 0.309443 4_1:-83976221 Msa0579310:CDS
TTTCCATAATTAGTCCAATT+AGG 0.311578 4_1:+83977017 None:intergenic
CTGGTGCTTGGGGCCTTTAT+GGG 0.316117 4_1:-83976210 Msa0579310:CDS
AGAAATGGCGAGAAGGTTTC+CGG 0.335298 4_1:+83978852 None:intergenic
CCACGATACAAAGACTGTTT+TGG 0.338175 4_1:+83976447 None:intergenic
GGTAATGTTAATGTTGATAT+TGG 0.343693 4_1:-83976300 Msa0579310:CDS
GCTTCTTCAATCAAACCTTT+AGG 0.347032 4_1:+83976267 None:intergenic
AATAACTACACTTGTGGAAA+TGG 0.347897 4_1:-83976594 Msa0579310:CDS
ATCAGGGTTGATTTCGGATT+GGG 0.356573 4_1:+83978909 None:intergenic
GCGGTGTACCCTATAGTTTA+AGG 0.356975 4_1:-83976977 Msa0579310:CDS
TGAGCACTTGGAACCTCTAT+TGG 0.358489 4_1:-83976682 Msa0579310:CDS
TCCACCTGGCAATCCGGTTC+CGG 0.362173 4_1:-83978994 Msa0579310:CDS
ACAATGTGAACCTTGCCCTT+TGG 0.362789 4_1:-83976653 Msa0579310:CDS
TAATAAAGATTTCTAGTTGC+TGG 0.363307 4_1:+83976909 None:intergenic
GGAGGAAGCGTACTGGTGCT+TGG 0.371645 4_1:-83976222 Msa0579310:CDS
GTAGGTTCTGACGAATTTCC+AGG 0.373251 4_1:+83976945 None:intergenic
TGAAGATTATTGAAAGATAG+AGG 0.374624 4_1:+83979205 None:intergenic
TCCATTGATTGTTGTGTACA+TGG 0.378076 4_1:+83976737 None:intergenic
AATGGCGTGGGCCGGAGAGC+AGG 0.380124 4_1:-83976576 Msa0579310:CDS
CATGCTTCAAGAAGTTATAC+TGG 0.380553 4_1:+83976817 None:intergenic
ATAACGATCAGGGTTGATTT+CGG 0.381601 4_1:+83978903 None:intergenic
CTTTATTACTCATTTGATAT+GGG 0.382012 4_1:-83976894 Msa0579310:CDS
GTTGCTCCCAAGAAGAAAAT+TGG 0.386841 4_1:+83976839 None:intergenic
GAGGGAGAGTGTGCCGGAAC+CGG 0.388793 4_1:+83978981 None:intergenic
TGGGGAGAGAAAATGTCATA+TGG 0.392406 4_1:-83978707 Msa0579310:CDS
AGGAAGAAGATACTACTATA+AGG 0.392651 4_1:-83978557 Msa0579310:intron
GTTTCGTTTGTTTCGGGTAA+AGG 0.396676 4_1:-83978823 Msa0579310:CDS
CATCTTGTGATTGGCATGGC+AGG 0.399832 4_1:-83976534 Msa0579310:CDS
CATGTTGAACCTTAAACTAT+AGG 0.406709 4_1:+83976968 None:intergenic
CCCACAACTCTATCAAAATC+CGG 0.407569 4_1:-83979141 Msa0579310:CDS
TTGAGGAAAGATCGAGTCAT+TGG 0.409792 4_1:+83976470 None:intergenic
TTCTCTCAACGCAACATCCT+TGG 0.412679 4_1:+83976713 None:intergenic
TTGTCATGGTCATAACGATC+AGG 0.414015 4_1:+83978892 None:intergenic
TATACCGTTCATCTTGTGAT+TGG 0.415416 4_1:-83976543 Msa0579310:CDS
AGAAAAGATGCTTGAGCACT+TGG 0.415912 4_1:-83976694 Msa0579310:CDS
TGGACTAATTATGGAAAAGA+TGG 0.416929 4_1:-83977011 Msa0579310:CDS
GCGAGAAGGTTTCCGGTTTG+TGG 0.419688 4_1:+83978859 None:intergenic
TGGATGATTTAGAGAGGAAG+AGG 0.425118 4_1:+83979013 None:intergenic
ACCGAGAATGTAACCCATAA+AGG 0.427471 4_1:+83976197 None:intergenic
AGTCTAGCTCTGAGGGTGAT+TGG 0.427544 4_1:+83979088 None:intergenic
GAAGTTGTCGTAGGAGGAGG+TGG 0.439677 4_1:+83979044 None:intergenic
TGTGCCGGAACCGGATTGCC+AGG 0.440070 4_1:+83978990 None:intergenic
TCCTCCTACGACAACTTCAT+TGG 0.441288 4_1:-83979039 Msa0579310:CDS
TATGGGTTACATTCTCGGTT+GGG 0.441433 4_1:-83976193 Msa0579310:CDS
ATTGGTAATGCTAAACCTAA+AGG 0.441714 4_1:-83976282 Msa0579310:CDS
ATGTTGAACCTTAAACTATA+GGG 0.442633 4_1:+83976969 None:intergenic
AAACGAAACCTCTTCAGAAA+TGG 0.443527 4_1:+83978837 None:intergenic
AAGCTCGTGGTTTCTTATGT+TGG 0.444757 4_1:-83976384 Msa0579310:CDS
TTAGGTTTCCATGGCTGTAA+AGG 0.446726 4_1:+83977035 None:intergenic
CTCCGTTCCAACTACATCTT+CGG 0.446774 4_1:-83978946 Msa0579310:CDS
AGGTTTCCGGTTTGTGGCAA+GGG 0.449186 4_1:+83978865 None:intergenic
TTATGGGTTACATTCTCGGT+TGG 0.451487 4_1:-83976194 Msa0579310:CDS
ACATTGTTGTTCACCAATAG+AGG 0.452089 4_1:+83976669 None:intergenic
TTGATCACTAGTTTAAAGAA+AGG 0.454168 4_1:-83978577 Msa0579310:CDS
AGTGTTGGTTGGAGGGATCC+AGG 0.454568 4_1:-83978616 Msa0579310:CDS
ATGGCGTGGGCCGGAGAGCA+GGG 0.457953 4_1:-83976575 Msa0579310:CDS
CCTTCTCGCCATTTCTGAAG+AGG 0.461630 4_1:-83978845 Msa0579310:CDS
TGCCCTTGAACTACTACAAA+AGG 0.467528 4_1:+83976765 None:intergenic
AGTTTAAGGTTCAACATGCC+TGG 0.468330 4_1:-83976963 Msa0579310:CDS
AAGGAGACGTATGTGAGGTA+TGG 0.469112 4_1:-83978727 Msa0579310:CDS
AATAACCCCTTGCCACAAAC+CGG 0.470128 4_1:-83978871 Msa0579310:CDS
CCTTAGTGTGACGGTGTCCC+CGG 0.471605 4_1:+83979122 None:intergenic
CCCATGTACACAACAATCAA+TGG 0.475166 4_1:-83976738 Msa0579310:CDS
CATGATTTCCAATGCATACA+TGG 0.476850 4_1:+83977069 None:intergenic
CAACCAACACTCTCGTTAGC+AGG 0.479587 4_1:+83978628 None:intergenic
TACATTATCCCAGTATGTCC+AGG 0.482507 4_1:-83976244 Msa0579310:CDS
GTGGCAAGGGGTTATTGTCA+TGG 0.483320 4_1:+83978878 None:intergenic
GGCAAGACAAGCAATCCATC+TGG 0.484465 4_1:-83976512 Msa0579310:CDS
GTTGGAACGGAGGTTGATGA+GGG 0.484503 4_1:+83978957 None:intergenic
AAATCATCCACCTGGCAATC+CGG 0.487160 4_1:-83979000 Msa0579310:CDS
TTGGAAATCATGAGTATGAT+TGG 0.491026 4_1:-83977058 Msa0579310:CDS
TACCTCACATACGTCTCCTT+CGG 0.495897 4_1:+83978730 None:intergenic
AAGGTTTCCGGTTTGTGGCA+AGG 0.496203 4_1:+83978864 None:intergenic
AGGAGACGTATGTGAGGTAT+GGG 0.496851 4_1:-83978726 Msa0579310:CDS
ACATCTTCGGTATCTTCCAT+TGG 0.497800 4_1:-83978933 Msa0579310:CDS
TGAGGAAAGATCGAGTCATT+GGG 0.499179 4_1:+83976471 None:intergenic
TTAGTCCAATTAGGTTTCCA+TGG 0.501745 4_1:+83977026 None:intergenic
ATCTATGAACATGGCCCCAA+AGG 0.501753 4_1:+83976637 None:intergenic
GATCAGGGTTGATTTCGGAT+TGG 0.501872 4_1:+83978908 None:intergenic
GTGGCATACCATGTATGCAT+TGG 0.503990 4_1:-83977077 Msa0579310:CDS
ATCCATCTGGAAAACAAGAC+CGG 0.506758 4_1:-83976499 Msa0579310:CDS
ATCCTTTGTCCCCTGCTCTC+CGG 0.507689 4_1:+83976565 None:intergenic
GCACCAGTACGCTTCCTCCC+TGG 0.509555 4_1:+83976226 None:intergenic
TTGCGGTGGCGCGTGTGAAG+AGG 0.510510 4_1:-83978678 Msa0579310:CDS
CTCTGAGGGTGATTGGATAC+CGG 0.512218 4_1:+83979095 None:intergenic
GGAAATTCGTCAGAACCTAC+TGG 0.514003 4_1:-83976942 Msa0579310:CDS
CAAGTGGTTATGCGTGGTTG+TGG 0.514084 4_1:-83977139 Msa0579310:CDS
GGGGAGAGAAAATGTCATAT+GGG 0.518872 4_1:-83978706 Msa0579310:CDS
ACCGTCACACTAAGGTGGTC+CGG 0.520460 4_1:-83979114 Msa0579310:CDS
TCTATGAACATGGCCCCAAA+GGG 0.523434 4_1:+83976638 None:intergenic
CTAACGAGAGTGTTGGTTGG+AGG 0.524469 4_1:-83978624 Msa0579310:CDS
CCACAACTCTATCAAAATCC+GGG 0.525087 4_1:-83979140 Msa0579310:CDS
GTTTGTTTCGGGTAAAGGAC+CGG 0.527184 4_1:-83978818 Msa0579310:CDS
ATGATTTGGAATCAGTTGAC+AGG 0.528504 4_1:-83976797 Msa0579310:CDS
TGAACCTGTTGCAACAAAAG+TGG 0.530909 4_1:-83977096 Msa0579310:CDS
AAGTGGTTATGCGTGGTTGT+GGG 0.532637 4_1:-83977138 Msa0579310:CDS
TCTTATGTTGGAAATCATGA+TGG 0.533637 4_1:-83976372 Msa0579310:CDS
AGTTGGAACGGAGGTTGATG+AGG 0.534951 4_1:+83978956 None:intergenic
CAAAACAGTCTTTGTATCGT+GGG 0.537627 4_1:-83976446 Msa0579310:CDS
AATGAAGTTGTCGTAGGAGG+AGG 0.537994 4_1:+83979041 None:intergenic
GAAGATACCGAAGATGTAGT+TGG 0.538252 4_1:+83978939 None:intergenic
TGGCGTGGGCCGGAGAGCAG+GGG 0.538311 4_1:-83976574 Msa0579310:CDS
ATGATTGGCCTTTACAGCCA+TGG 0.542059 4_1:-83977043 Msa0579310:CDS
CTCCGTGATGTTGAAGCTCT+AGG 0.548924 4_1:-83977206 Msa0579310:CDS
GGCCGGAGAGCAGGGGACAA+AGG 0.549021 4_1:-83976567 Msa0579310:CDS
CCATTGATTGTTGTGTACAT+GGG 0.550156 4_1:+83976738 None:intergenic
ATCTTGTGATTGGCATGGCA+GGG 0.555722 4_1:-83976533 Msa0579310:CDS
TGATTTCGGATTGGGTCCAA+TGG 0.556134 4_1:+83978917 None:intergenic
AAAGCATATCAACAGTGAAG+TGG 0.556245 4_1:-83977232 Msa0579310:CDS
GCCAATGAAGTTGTCGTAGG+AGG 0.560684 4_1:+83979038 None:intergenic
AAAGCAAGATGAATCTGCTC+CGG 0.560908 4_1:+83978799 None:intergenic
CTGCTAACGAGAGTGTTGGT+TGG 0.561825 4_1:-83978627 Msa0579310:CDS
CCTCTTCAGAAATGGCGAGA+AGG 0.567798 4_1:+83978845 None:intergenic
TAACGAGAGTGTTGGTTGGA+GGG 0.568916 4_1:-83978623 Msa0579310:CDS
CCACAAGTGTAGTTATTCAG+TGG 0.569232 4_1:+83976600 None:intergenic
CCGGGGACACCGTCACACTA+AGG 0.569874 4_1:-83979122 Msa0579310:CDS
ATCGAGTCATTGGGATGACC+CGG 0.570194 4_1:+83976480 None:intergenic
TGTCCAGGGAGGAAGCGTAC+TGG 0.570210 4_1:-83976229 Msa0579310:CDS
ATCTCTCGTATCTATGAACA+TGG 0.572154 4_1:+83976628 None:intergenic
TGTCATGGTCATAACGATCA+GGG 0.575204 4_1:+83978893 None:intergenic
CAACTCATAGCTTTGTGTCA+AGG 0.575696 4_1:-83977343 Msa0579310:CDS
CCACTGAATAACTACACTTG+TGG 0.577944 4_1:-83976600 Msa0579310:CDS
CTTGTGGAAATGGCGTGGGC+CGG 0.578247 4_1:-83976584 Msa0579310:CDS
TCGGTTGGGTTATACATGCA+AGG 0.580242 4_1:-83976179 Msa0579310:CDS
TTCCTAGAGCTTCAACATCA+CGG 0.580505 4_1:+83977204 None:intergenic
CGTTCATCTTGTGATTGGCA+TGG 0.581543 4_1:-83976538 Msa0579310:CDS
ATGTCATATGGGAGGTCTTG+CGG 0.583227 4_1:-83978695 Msa0579310:CDS
TAGAGGAAGAGTATTGTGCA+AGG 0.584858 4_1:+83979222 None:intergenic
GAACATGGCCCCAAAGGGCA+AGG 0.584999 4_1:+83976643 None:intergenic
GGATGAAGAAGATGCTATGC+GGG 0.585911 4_1:-83978773 Msa0579310:CDS
GTAGAAACACTCCTGGACTC+TGG 0.586121 4_1:+83976150 None:intergenic
GCTTCCTCCCTGGACATACT+GGG 0.586632 4_1:+83976236 None:intergenic
ACTAATTATGGAAAAGATGG+AGG 0.587578 4_1:-83977008 Msa0579310:CDS
TACACTTGTGGAAATGGCGT+GGG 0.587976 4_1:-83976588 Msa0579310:CDS
CCAAAACAGTCTTTGTATCG+TGG 0.588649 4_1:-83976447 Msa0579310:CDS
GCTACGAAGTCTAGCTCTGA+GGG 0.589676 4_1:+83979081 None:intergenic
CCGATGTGTGACACAAAAGC+GGG 0.592792 4_1:+83977176 None:intergenic
TACCGAAGATGTAGTTGGAA+CGG 0.595020 4_1:+83978944 None:intergenic
AATTATGGAAAAGATGGAGG+TGG 0.595238 4_1:-83977005 Msa0579310:CDS
CTACACTTGTGGAAATGGCG+TGG 0.595264 4_1:-83976589 Msa0579310:CDS
AAAGCATCATGTATATAACC+TGG 0.595486 4_1:+83978598 None:intergenic
TCCATCTGGAAAACAAGACC+GGG 0.597952 4_1:-83976498 Msa0579310:CDS
GGTTTCCGGTTTGTGGCAAG+GGG 0.598797 4_1:+83978866 None:intergenic
CGTGTGAGTAGAAACACTCC+TGG 0.600020 4_1:+83976143 None:intergenic
AGGAAGCGTACTGGTGCTTG+GGG 0.600088 4_1:-83976220 Msa0579310:CDS
GTATGTGACGAGGGATCCGA+AGG 0.606007 4_1:-83978746 Msa0579310:CDS
CACAACAATCAATGGAACCA+AGG 0.611304 4_1:-83976730 Msa0579310:CDS
TCCTCTCTAAATCATCCACC+TGG 0.615171 4_1:-83979008 Msa0579310:CDS
TCCGATGTGTGACACAAAAG+CGG 0.615605 4_1:+83977175 None:intergenic
AAAACAGTCTTTGTATCGTG+GGG 0.615897 4_1:-83976445 Msa0579310:CDS
CGCGTGTGAAGAGGTATGAG+AGG 0.617971 4_1:-83978669 Msa0579310:CDS
TGGAACGGAGGTTGATGAGG+GGG 0.618753 4_1:+83978959 None:intergenic
GTTTGGGTACATTAGACTAG+TGG 0.624624 4_1:-83976418 Msa0579310:CDS
GTAGCCAATGAAGTTGTCGT+AGG 0.625292 4_1:+83979035 None:intergenic
ACCGGACCACCTTAGTGTGA+CGG 0.627721 4_1:+83979113 None:intergenic
TTGTCGTAGGAGGAGGTGGG+TGG 0.632479 4_1:+83979048 None:intergenic
AAAGATGGAGGTGGTGAGTG+CGG 0.636261 4_1:-83976996 Msa0579310:CDS
GCCAGGTGGATGATTTAGAG+AGG 0.636449 4_1:+83979007 None:intergenic
GCTGGAGCTATAGTTCCAGT+AGG 0.636710 4_1:+83976927 None:intergenic
ACATTATCCCAGTATGTCCA+GGG 0.637529 4_1:-83976243 Msa0579310:CDS
AAGTTGTCGTAGGAGGAGGT+GGG 0.638967 4_1:+83979045 None:intergenic
GCGGGTTATGTATGTGACGA+GGG 0.644902 4_1:-83978755 Msa0579310:CDS
TTGGAACGGAGGTTGATGAG+GGG 0.645080 4_1:+83978958 None:intergenic
GGAGACATTAGTTACGCAAG+TGG 0.646032 4_1:-83977155 Msa0579310:CDS
AATGTGAACCTTGCCCTTTG+GGG 0.650716 4_1:-83976651 Msa0579310:CDS
TGCGGGTTATGTATGTGACG+AGG 0.651449 4_1:-83978756 Msa0579310:CDS
GGGACACCGTCACACTAAGG+TGG 0.653790 4_1:-83979119 Msa0579310:CDS
TGCTACGAAGTCTAGCTCTG+AGG 0.656528 4_1:+83979080 None:intergenic
GTTACGCAAGTGGTTATGCG+TGG 0.658494 4_1:-83977145 Msa0579310:CDS
CATGCCAATCACAAGATGAA+CGG 0.659360 4_1:+83976539 None:intergenic
GCGTGTGAAGAGGTATGAGA+GGG 0.660850 4_1:-83978668 Msa0579310:CDS
GGCTACAAAGCAGAAGCTCG+TGG 0.661109 4_1:-83976397 Msa0579310:CDS
GCTCCTGCTAACGAGAGTGT+TGG 0.663854 4_1:-83978631 Msa0579310:CDS
CACAACTCTATCAAAATCCG+GGG 0.669749 4_1:-83979139 Msa0579310:CDS
CGGATGAAGAAGATGCTATG+CGG 0.672413 4_1:-83978774 Msa0579310:CDS
ATCCGAAGGAGACGTATGTG+AGG 0.673765 4_1:-83978732 Msa0579310:CDS
GAGAGAAAATGTCATATGGG+AGG 0.677448 4_1:-83978703 Msa0579310:CDS
CGAAGATGTAGTTGGAACGG+AGG 0.680198 4_1:+83978947 None:intergenic
TCATATGGGAGGTCTTGCGG+TGG 0.686577 4_1:-83978692 Msa0579310:CDS
TGTTGGAAATCATGATGGTG+AGG 0.687796 4_1:-83976367 Msa0579310:CDS
GCCGGAACCGGATTGCCAGG+TGG 0.689201 4_1:+83978993 None:intergenic
GGAGACGTATGTGAGGTATG+GGG 0.695565 4_1:-83978725 Msa0579310:CDS
TGGTGAGGTGCATGACACAA+TGG 0.697263 4_1:-83976352 Msa0579310:CDS
AAACAGTCTTTGTATCGTGG+GGG 0.711447 4_1:-83976444 Msa0579310:CDS
CTAGAGCTTCAACATCACGG+AGG 0.777257 4_1:+83977207 None:intergenic
TTATCCCAGTATGTCCAGGG+AGG 0.778942 4_1:-83976240 Msa0579310:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTATTTTTTAATAATA+AGG - chr4_1:83977744-83977763 Msa0579310:intron 0.0%
!! AATAATGAAAGTAAAAAACA+AGG + chr4_1:83976893-83976912 None:intergenic 15.0%
!!! TTTTGCTTAATTTTATTGAA+TGG - chr4_1:83977513-83977532 Msa0579310:intron 15.0%
!!! TTTGCTTAATTTTATTGAAT+GGG - chr4_1:83977514-83977533 Msa0579310:intron 15.0%
!! AAAATTATGATAGAAAACAA+AGG - chr4_1:83977804-83977823 Msa0579310:intron 15.0%
!!! AAAACAAAATCATTGTTTTA+AGG - chr4_1:83977867-83977886 Msa0579310:intron 15.0%
!! TTTCATTATTACTTTGTGTT+TGG - chr4_1:83976903-83976922 Msa0579310:CDS 20.0%
!!! AAAATACCTCGATATTTTTT+TGG - chr4_1:83977144-83977163 Msa0579310:CDS 20.0%
!!! AAATACCTCGATATTTTTTT+GGG - chr4_1:83977145-83977164 Msa0579310:CDS 20.0%
!!! TGGAAAAGATTTTTAAGTAT+TGG - chr4_1:83977597-83977616 Msa0579310:intron 20.0%
!! TTGTATATCCAAATATAGTT+GGG + chr4_1:83977657-83977676 None:intergenic 20.0%
!! ATTGTATATCCAAATATAGT+TGG + chr4_1:83977658-83977677 None:intergenic 20.0%
!! TTGTATATCCAAATATAGTT+GGG + chr4_1:83977657-83977676 None:intergenic 20.0%
!! ATTGTATATCCAAATATAGT+TGG + chr4_1:83977658-83977677 None:intergenic 20.0%
!! TCTTTATTACTCATTTGATA+TGG - chr4_1:83978455-83978474 Msa0579310:intron 20.0%
!! CTTTATTACTCATTTGATAT+GGG - chr4_1:83978456-83978475 Msa0579310:intron 20.0%
! TGAAGATTATTGAAAGATAG+AGG + chr4_1:83976148-83976167 None:intergenic 25.0%
!! TTGATCACTAGTTTAAAGAA+AGG - chr4_1:83976773-83976792 Msa0579310:CDS 25.0%
! ATGAAAGTAAAAAACAAGGT+AGG + chr4_1:83976889-83976908 None:intergenic 25.0%
! ATTATTACTTTGTGTTTGGT+TGG - chr4_1:83976907-83976926 Msa0579310:CDS 25.0%
!! GGTAATGGTTTAAAGTTTAA+AGG - chr4_1:83976979-83976998 Msa0579310:CDS 25.0%
!!! TTTGATTTGATAAAGAGACA+AGG - chr4_1:83977173-83977192 Msa0579310:CDS 25.0%
! CACTAAATCATAAGAAAACT+TGG - chr4_1:83977297-83977316 Msa0579310:CDS 25.0%
!!! ATTTTTGATAGTTTGCGTTA+TGG + chr4_1:83977407-83977426 None:intergenic 25.0%
!!! AAGATTTTTAAGTATTGGTC+TGG - chr4_1:83977602-83977621 Msa0579310:intron 25.0%
! TCATGTAATCGTTTAGTATA+TGG - chr4_1:83977916-83977935 Msa0579310:intron 25.0%
!! GAAACAATAGCTTTCATTTT+TGG - chr4_1:83978045-83978064 Msa0579310:intron 25.0%
! GTACGCAAAAATGTATTATA+TGG + chr4_1:83978090-83978109 None:intergenic 25.0%
! AAACCTAATTGGACTAATTA+TGG - chr4_1:83978330-83978349 Msa0579310:intron 25.0%
! TTTCCATAATTAGTCCAATT+AGG + chr4_1:83978336-83978355 None:intergenic 25.0%
! ATGTTGAACCTTAAACTATA+GGG + chr4_1:83978384-83978403 None:intergenic 25.0%
!!! TAATAAAGATTTCTAGTTGC+TGG + chr4_1:83978444-83978463 None:intergenic 25.0%
! GGTAATGTTAATGTTGATAT+TGG - chr4_1:83979050-83979069 Msa0579310:CDS 25.0%
! AAAGCATCATGTATATAACC+TGG + chr4_1:83976755-83976774 None:intergenic 30.0%
AGGAAGAAGATACTACTATA+AGG - chr4_1:83976793-83976812 Msa0579310:CDS 30.0%
CAAGGTAATCAAAATTAGAC+CGG - chr4_1:83976934-83976953 Msa0579310:CDS 30.0%
! CTTTAAACATGTGTTGGTAA+TGG - chr4_1:83976958-83976977 Msa0579310:CDS 30.0%
GGAGAACTAAAATCGTATTT+CGG + chr4_1:83977431-83977450 None:intergenic 30.0%
!! ATATAGTTGGGTTCTAAACA+TGG + chr4_1:83977697-83977716 None:intergenic 30.0%
! GTTTAGAACCCAACTATATT+TGG - chr4_1:83977698-83977717 Msa0579310:intron 30.0%
GATTACATGAACCAGATTTA+GGG + chr4_1:83977906-83977925 None:intergenic 30.0%
! TACCAGGTTGGAAATAAAAA+TGG - chr4_1:83977973-83977992 Msa0579310:intron 30.0%
TTGGAAATAAAAATGGAGGT+TGG - chr4_1:83977980-83977999 Msa0579310:intron 30.0%
TTGGAAATCATGAGTATGAT+TGG - chr4_1:83978292-83978311 Msa0579310:intron 30.0%
TGGACTAATTATGGAAAAGA+TGG - chr4_1:83978339-83978358 Msa0579310:intron 30.0%
ACTAATTATGGAAAAGATGG+AGG - chr4_1:83978342-83978361 Msa0579310:intron 30.0%
CATGTTGAACCTTAAACTAT+AGG + chr4_1:83978385-83978404 None:intergenic 30.0%
TAACTTCTTGAAGCATGATT+TGG - chr4_1:83978539-83978558 Msa0579310:intron 30.0%
AATAACTACACTTGTGGAAA+TGG - chr4_1:83978756-83978775 Msa0579310:CDS 30.0%
TCTTATGTTGGAAATCATGA+TGG - chr4_1:83978978-83978997 Msa0579310:CDS 30.0%
!! ATTGGTAATGCTAAACCTAA+AGG - chr4_1:83979068-83979087 Msa0579310:CDS 30.0%
! ATAACGATCAGGGTTGATTT+CGG + chr4_1:83976450-83976469 None:intergenic 35.0%
AAACGAAACCTCTTCAGAAA+TGG + chr4_1:83976516-83976535 None:intergenic 35.0%
TGAAGAGGTTTCGTTTGTTT+CGG - chr4_1:83976520-83976539 Msa0579310:CDS 35.0%
TTGTGTTTGGTTGGAAAACA+AGG - chr4_1:83976916-83976935 Msa0579310:CDS 35.0%
! ACCAACACATGTTTAAAGTC+CGG + chr4_1:83976956-83976975 None:intergenic 35.0%
!! ACATGTGTTGGTAATGGTAA+TGG - chr4_1:83976964-83976983 Msa0579310:CDS 35.0%
ACACACCCAAAAAAATATCG+AGG + chr4_1:83977153-83977172 None:intergenic 35.0%
! ACCCTGTTTGATTTACAAAC+TGG - chr4_1:83977216-83977235 Msa0579310:CDS 35.0%
GCCAGTTTGTAAATCAAACA+GGG + chr4_1:83977220-83977239 None:intergenic 35.0%
TTGATTTACAAACTGGCCAT+TGG - chr4_1:83977223-83977242 Msa0579310:CDS 35.0%
!!! ACTTGTTTTGTCTAGTCCAA+TGG + chr4_1:83977242-83977261 None:intergenic 35.0%
TCATAAGAAAACTTGGCCAT+TGG - chr4_1:83977304-83977323 Msa0579310:CDS 35.0%
!!! ACTTGTTTTGTCTAGTCCAA+TGG + chr4_1:83977242-83977261 None:intergenic 35.0%
CCAAATATAGTTGGGTTCTA+CGG + chr4_1:83977649-83977668 None:intergenic 35.0%
CGATTACATGAACCAGATTT+AGG + chr4_1:83977907-83977926 None:intergenic 35.0%
CATCACTCAAGTTAAGTTTC+TGG + chr4_1:83977945-83977964 None:intergenic 35.0%
! CAGAAACTTAACTTGAGTGA+TGG - chr4_1:83977943-83977962 Msa0579310:intron 35.0%
!! CTCCATTTTTATTTCCAACC+TGG + chr4_1:83977978-83977997 None:intergenic 35.0%
! CAGGTTGGAAATAAAAATGG+AGG - chr4_1:83977976-83977995 Msa0579310:intron 35.0%
!!! AGCTTTCATTTTTGGTGACA+TGG - chr4_1:83978053-83978072 Msa0579310:intron 35.0%
!!! GCTTTCATTTTTGGTGACAT+GGG - chr4_1:83978054-83978073 Msa0579310:intron 35.0%
AAAGCATATCAACAGTGAAG+TGG - chr4_1:83978118-83978137 Msa0579310:intron 35.0%
CATGATTTCCAATGCATACA+TGG + chr4_1:83978284-83978303 None:intergenic 35.0%
TTAGTCCAATTAGGTTTCCA+TGG + chr4_1:83978327-83978346 None:intergenic 35.0%
AATTATGGAAAAGATGGAGG+TGG - chr4_1:83978345-83978364 Msa0579310:intron 35.0%
! ACAGAGACCAATTTTCTTCT+TGG - chr4_1:83978504-83978523 Msa0579310:intron 35.0%
! CAGAGACCAATTTTCTTCTT+GGG - chr4_1:83978505-83978524 Msa0579310:intron 35.0%
CATGCTTCAAGAAGTTATAC+TGG + chr4_1:83978536-83978555 None:intergenic 35.0%
! ATGATTTGGAATCAGTTGAC+AGG - chr4_1:83978553-83978572 Msa0579310:intron 35.0%
! ACTCCTTTTGTAGTAGTTCA+AGG - chr4_1:83978582-83978601 Msa0579310:CDS 35.0%
! CTCCTTTTGTAGTAGTTCAA+GGG - chr4_1:83978583-83978602 Msa0579310:CDS 35.0%
! CCATTGATTGTTGTGTACAT+GGG + chr4_1:83978615-83978634 None:intergenic 35.0%
! TCCATTGATTGTTGTGTACA+TGG + chr4_1:83978616-83978635 None:intergenic 35.0%
! ACATTGTTGTTCACCAATAG+AGG + chr4_1:83978684-83978703 None:intergenic 35.0%
ATCTCTCGTATCTATGAACA+TGG + chr4_1:83978725-83978744 None:intergenic 35.0%
TATACCGTTCATCTTGTGAT+TGG - chr4_1:83978807-83978826 Msa0579310:CDS 35.0%
!!! TACAAAGACTGTTTTGGTTG+AGG + chr4_1:83978900-83978919 None:intergenic 35.0%
CAAAACAGTCTTTGTATCGT+GGG - chr4_1:83978904-83978923 Msa0579310:CDS 35.0%
AAAACAGTCTTTGTATCGTG+GGG - chr4_1:83978905-83978924 Msa0579310:CDS 35.0%
! GCATGACACAATGGAAATTT+TGG - chr4_1:83979007-83979026 Msa0579310:CDS 35.0%
!! ACAATGGAAATTTTGGCATC+TGG - chr4_1:83979014-83979033 Msa0579310:CDS 35.0%
GCTTCTTCAATCAAACCTTT+AGG + chr4_1:83979086-83979105 None:intergenic 35.0%
!! TAGAGGAAGAGTATTGTGCA+AGG + chr4_1:83976131-83976150 None:intergenic 40.0%
!! GGATTTTGATAGAGTTGTGG+GGG + chr4_1:83976210-83976229 None:intergenic 40.0%
!! CGGATTTTGATAGAGTTGTG+GGG + chr4_1:83976211-83976230 None:intergenic 40.0%
!! CCGGATTTTGATAGAGTTGT+GGG + chr4_1:83976212-83976231 None:intergenic 40.0%
CCCACAACTCTATCAAAATC+CGG - chr4_1:83976209-83976228 Msa0579310:CDS 40.0%
CCACAACTCTATCAAAATCC+GGG - chr4_1:83976210-83976229 Msa0579310:CDS 40.0%
CACAACTCTATCAAAATCCG+GGG - chr4_1:83976211-83976230 Msa0579310:CDS 40.0%
TGGATGATTTAGAGAGGAAG+AGG + chr4_1:83976340-83976359 None:intergenic 40.0%
TACCGAAGATGTAGTTGGAA+CGG + chr4_1:83976409-83976428 None:intergenic 40.0%
GAAGATACCGAAGATGTAGT+TGG + chr4_1:83976414-83976433 None:intergenic 40.0%
ACATCTTCGGTATCTTCCAT+TGG - chr4_1:83976417-83976436 Msa0579310:CDS 40.0%
! ATCAGGGTTGATTTCGGATT+GGG + chr4_1:83976444-83976463 None:intergenic 40.0%
TGTCATGGTCATAACGATCA+GGG + chr4_1:83976460-83976479 None:intergenic 40.0%
TTGTCATGGTCATAACGATC+AGG + chr4_1:83976461-83976480 None:intergenic 40.0%
GAAGAGGTTTCGTTTGTTTC+GGG - chr4_1:83976521-83976540 Msa0579310:CDS 40.0%
! GTTTCGTTTGTTTCGGGTAA+AGG - chr4_1:83976527-83976546 Msa0579310:CDS 40.0%
AAAGCAAGATGAATCTGCTC+CGG + chr4_1:83976554-83976573 None:intergenic 40.0%
!! GCAGATTCATCTTGCTTTTG+CGG - chr4_1:83976556-83976575 Msa0579310:CDS 40.0%
TGGGGAGAGAAAATGTCATA+TGG - chr4_1:83976643-83976662 Msa0579310:CDS 40.0%
GGGGAGAGAAAATGTCATAT+GGG - chr4_1:83976644-83976663 Msa0579310:CDS 40.0%
GAGAGAAAATGTCATATGGG+AGG - chr4_1:83976647-83976666 Msa0579310:CDS 40.0%
ACCGGACTTTAAACATGTGT+TGG - chr4_1:83976952-83976971 Msa0579310:CDS 40.0%
GGCCAGTTTGTAAATCAAAC+AGG + chr4_1:83977221-83977240 None:intergenic 40.0%
ATCATCGTCTAGTGAGAAAG+AGG - chr4_1:83977538-83977557 Msa0579310:intron 40.0%
TGAGAAAGAGGATCATGAGT+TGG - chr4_1:83977550-83977569 Msa0579310:intron 40.0%
GAGAAAGAGGATCATGAGTT+GGG - chr4_1:83977551-83977570 Msa0579310:intron 40.0%
!! TCATCGATTGCTGTTTGAGT+TGG - chr4_1:83977577-83977596 Msa0579310:intron 40.0%
CCGTAGAACCCAACTATATT+TGG - chr4_1:83977646-83977665 Msa0579310:intron 40.0%
AAACAAACACGCCCTAAATC+TGG - chr4_1:83977892-83977911 Msa0579310:intron 40.0%
!! CAACTCATAGCTTTGTGTCA+AGG - chr4_1:83978007-83978026 Msa0579310:intron 40.0%
TTCCTAGAGCTTCAACATCA+CGG + chr4_1:83978149-83978168 None:intergenic 40.0%
TGAACCTGTTGCAACAAAAG+TGG - chr4_1:83978254-83978273 Msa0579310:intron 40.0%
!!! TATGCCACTTTTGTTGCAAC+AGG + chr4_1:83978261-83978280 None:intergenic 40.0%
TTAGGTTTCCATGGCTGTAA+AGG + chr4_1:83978318-83978337 None:intergenic 40.0%
TACAGCCATGGAAACCTAAT+TGG - chr4_1:83978319-83978338 Msa0579310:intron 40.0%
! AGTTTAAGGTTCAACATGCC+TGG - chr4_1:83978387-83978406 Msa0579310:intron 40.0%
GTTGCTCCCAAGAAGAAAAT+TGG + chr4_1:83978514-83978533 None:intergenic 40.0%
TGCCCTTGAACTACTACAAA+AGG + chr4_1:83978588-83978607 None:intergenic 40.0%
CCCATGTACACAACAATCAA+TGG - chr4_1:83978612-83978631 Msa0579310:CDS 40.0%
CACAACAATCAATGGAACCA+AGG - chr4_1:83978620-83978639 Msa0579310:CDS 40.0%
AGAAAAGATGCTTGAGCACT+TGG - chr4_1:83978656-83978675 Msa0579310:CDS 40.0%
CCACAAGTGTAGTTATTCAG+TGG + chr4_1:83978753-83978772 None:intergenic 40.0%
CCACTGAATAACTACACTTG+TGG - chr4_1:83978750-83978769 Msa0579310:CDS 40.0%
CATGCCAATCACAAGATGAA+CGG + chr4_1:83978814-83978833 None:intergenic 40.0%
ATCCATCTGGAAAACAAGAC+CGG - chr4_1:83978851-83978870 Msa0579310:CDS 40.0%
TGAGGAAAGATCGAGTCATT+GGG + chr4_1:83978882-83978901 None:intergenic 40.0%
TTGAGGAAAGATCGAGTCAT+TGG + chr4_1:83978883-83978902 None:intergenic 40.0%
! CCACGATACAAAGACTGTTT+TGG + chr4_1:83978906-83978925 None:intergenic 40.0%
CCAAAACAGTCTTTGTATCG+TGG - chr4_1:83978903-83978922 Msa0579310:CDS 40.0%
AAACAGTCTTTGTATCGTGG+GGG - chr4_1:83978906-83978925 Msa0579310:CDS 40.0%
GTTTGGGTACATTAGACTAG+TGG - chr4_1:83978932-83978951 Msa0579310:CDS 40.0%
AAGCTCGTGGTTTCTTATGT+TGG - chr4_1:83978966-83978985 Msa0579310:CDS 40.0%
! TGTTGGAAATCATGATGGTG+AGG - chr4_1:83978983-83979002 Msa0579310:CDS 40.0%
! GCATCTGGAGAACTTTTCAA+TGG - chr4_1:83979029-83979048 Msa0579310:CDS 40.0%
TACATTATCCCAGTATGTCC+AGG - chr4_1:83979106-83979125 Msa0579310:CDS 40.0%
ACATTATCCCAGTATGTCCA+GGG - chr4_1:83979107-83979126 Msa0579310:CDS 40.0%
! GCCTTTATGGGTTACATTCT+CGG - chr4_1:83979152-83979171 Msa0579310:CDS 40.0%
ACCGAGAATGTAACCCATAA+AGG + chr4_1:83979156-83979175 None:intergenic 40.0%
! TTATGGGTTACATTCTCGGT+TGG - chr4_1:83979156-83979175 Msa0579310:CDS 40.0%
! TATGGGTTACATTCTCGGTT+GGG - chr4_1:83979157-83979176 Msa0579310:CDS 40.0%
!!! GGGTTTTGATTGGACAAGGT+TGG + chr4_1:83976174-83976193 None:intergenic 45.0%
TGATAGAGTTGTGGGGGTTA+CGG + chr4_1:83976204-83976223 None:intergenic 45.0%
!! CCCGGATTTTGATAGAGTTG+TGG + chr4_1:83976213-83976232 None:intergenic 45.0%
AATGAAGTTGTCGTAGGAGG+AGG + chr4_1:83976312-83976331 None:intergenic 45.0%
TCCTCCTACGACAACTTCAT+TGG - chr4_1:83976311-83976330 Msa0579310:CDS 45.0%
GTAGCCAATGAAGTTGTCGT+AGG + chr4_1:83976318-83976337 None:intergenic 45.0%
TCCTCTCTAAATCATCCACC+TGG - chr4_1:83976342-83976361 Msa0579310:CDS 45.0%
AAATCATCCACCTGGCAATC+CGG - chr4_1:83976350-83976369 Msa0579310:CDS 45.0%
CTCCGTTCCAACTACATCTT+CGG - chr4_1:83976404-83976423 Msa0579310:CDS 45.0%
! TGATTTCGGATTGGGTCCAA+TGG + chr4_1:83976436-83976455 None:intergenic 45.0%
! GATCAGGGTTGATTTCGGAT+TGG + chr4_1:83976445-83976464 None:intergenic 45.0%
AATAACCCCTTGCCACAAAC+CGG - chr4_1:83976479-83976498 Msa0579310:CDS 45.0%
AGAAATGGCGAGAAGGTTTC+CGG + chr4_1:83976501-83976520 None:intergenic 45.0%
! GTTTGTTTCGGGTAAAGGAC+CGG - chr4_1:83976532-83976551 Msa0579310:CDS 45.0%
CGGATGAAGAAGATGCTATG+CGG - chr4_1:83976576-83976595 Msa0579310:CDS 45.0%
GGATGAAGAAGATGCTATGC+GGG - chr4_1:83976577-83976596 Msa0579310:CDS 45.0%
TACCTCACATACGTCTCCTT+CGG + chr4_1:83976623-83976642 None:intergenic 45.0%
AAGGAGACGTATGTGAGGTA+TGG - chr4_1:83976623-83976642 Msa0579310:CDS 45.0%
AGGAGACGTATGTGAGGTAT+GGG - chr4_1:83976624-83976643 Msa0579310:CDS 45.0%
ATGTCATATGGGAGGTCTTG+CGG - chr4_1:83976655-83976674 Msa0579310:CDS 45.0%
!! TAACGAGAGTGTTGGTTGGA+GGG - chr4_1:83976727-83976746 Msa0579310:CDS 45.0%
! TACGGTTGACTAGCTGACAA+GGG + chr4_1:83977631-83977650 None:intergenic 45.0%
TCCGATGTGTGACACAAAAG+CGG + chr4_1:83978178-83978197 None:intergenic 45.0%
GGAGACATTAGTTACGCAAG+TGG - chr4_1:83978195-83978214 Msa0579310:intron 45.0%
AAGTGGTTATGCGTGGTTGT+GGG - chr4_1:83978212-83978231 Msa0579310:intron 45.0%
GTGGCATACCATGTATGCAT+TGG - chr4_1:83978273-83978292 Msa0579310:intron 45.0%
!! ATGATTGGCCTTTACAGCCA+TGG - chr4_1:83978307-83978326 Msa0579310:intron 45.0%
GCGGTGTACCCTATAGTTTA+AGG - chr4_1:83978373-83978392 Msa0579310:intron 45.0%
! GTAGGTTCTGACGAATTTCC+AGG + chr4_1:83978408-83978427 None:intergenic 45.0%
GGAAATTCGTCAGAACCTAC+TGG - chr4_1:83978408-83978427 Msa0579310:intron 45.0%
TTCTCTCAACGCAACATCCT+TGG + chr4_1:83978640-83978659 None:intergenic 45.0%
TGAGCACTTGGAACCTCTAT+TGG - chr4_1:83978668-83978687 Msa0579310:CDS 45.0%
ACAATGTGAACCTTGCCCTT+TGG - chr4_1:83978697-83978716 Msa0579310:CDS 45.0%
CAATGTGAACCTTGCCCTTT+GGG - chr4_1:83978698-83978717 Msa0579310:CDS 45.0%
AATGTGAACCTTGCCCTTTG+GGG - chr4_1:83978699-83978718 Msa0579310:CDS 45.0%
TCTATGAACATGGCCCCAAA+GGG + chr4_1:83978715-83978734 None:intergenic 45.0%
ATCTATGAACATGGCCCCAA+AGG + chr4_1:83978716-83978735 None:intergenic 45.0%
TACACTTGTGGAAATGGCGT+GGG - chr4_1:83978762-83978781 Msa0579310:CDS 45.0%
CGTTCATCTTGTGATTGGCA+TGG - chr4_1:83978812-83978831 Msa0579310:CDS 45.0%
ATCTTGTGATTGGCATGGCA+GGG - chr4_1:83978817-83978836 Msa0579310:CDS 45.0%
TCCATCTGGAAAACAAGACC+GGG - chr4_1:83978852-83978871 Msa0579310:CDS 45.0%
TCGGTTGGGTTATACATGCA+AGG - chr4_1:83979171-83979190 Msa0579310:CDS 45.0%
CAAGGAAAAAACCAGAGTCC+AGG - chr4_1:83979189-83979208 Msa0579310:CDS 45.0%
!!! ATATACATTTTAAAAATTAT+AGG + chr4_1:83977007-83977026 None:intergenic 5.0%
!! ATAAAAAAAAAATATATTTG+AGG - chr4_1:83977095-83977114 Msa0579310:CDS 5.0%
!!! ATTTATAATATTTTTTATGT+TGG + chr4_1:83977452-83977471 None:intergenic 5.0%
!!! GGGAGGGTTTTGATTGGACA+AGG + chr4_1:83976178-83976197 None:intergenic 50.0%
!! CGGTTAGGGAGGGTTTTGAT+TGG + chr4_1:83976184-83976203 None:intergenic 50.0%
AGTTGTGGGGGTTACGGTTA+GGG + chr4_1:83976198-83976217 None:intergenic 50.0%
! CTCTGAGGGTGATTGGATAC+CGG + chr4_1:83976258-83976277 None:intergenic 50.0%
!! AGTCTAGCTCTGAGGGTGAT+TGG + chr4_1:83976265-83976284 None:intergenic 50.0%
!! GCTACGAAGTCTAGCTCTGA+GGG + chr4_1:83976272-83976291 None:intergenic 50.0%
!! TGCTACGAAGTCTAGCTCTG+AGG + chr4_1:83976273-83976292 None:intergenic 50.0%
AAGTTGTCGTAGGAGGAGGT+GGG + chr4_1:83976308-83976327 None:intergenic 50.0%
GCCAATGAAGTTGTCGTAGG+AGG + chr4_1:83976315-83976334 None:intergenic 50.0%
GCCAGGTGGATGATTTAGAG+AGG + chr4_1:83976346-83976365 None:intergenic 50.0%
!! TTGGAACGGAGGTTGATGAG+GGG + chr4_1:83976395-83976414 None:intergenic 50.0%
!! GTTGGAACGGAGGTTGATGA+GGG + chr4_1:83976396-83976415 None:intergenic 50.0%
!! AGTTGGAACGGAGGTTGATG+AGG + chr4_1:83976397-83976416 None:intergenic 50.0%
CGAAGATGTAGTTGGAACGG+AGG + chr4_1:83976406-83976425 None:intergenic 50.0%
GTGGCAAGGGGTTATTGTCA+TGG + chr4_1:83976475-83976494 None:intergenic 50.0%
! AGGTTTCCGGTTTGTGGCAA+GGG + chr4_1:83976488-83976507 None:intergenic 50.0%
! AAGGTTTCCGGTTTGTGGCA+AGG + chr4_1:83976489-83976508 None:intergenic 50.0%
CCTCTTCAGAAATGGCGAGA+AGG + chr4_1:83976508-83976527 None:intergenic 50.0%
! CCTTCTCGCCATTTCTGAAG+AGG - chr4_1:83976505-83976524 Msa0579310:CDS 50.0%
! TGCGGGTTATGTATGTGACG+AGG - chr4_1:83976594-83976613 Msa0579310:CDS 50.0%
! GCGGGTTATGTATGTGACGA+GGG - chr4_1:83976595-83976614 Msa0579310:CDS 50.0%
ATCCGAAGGAGACGTATGTG+AGG - chr4_1:83976618-83976637 Msa0579310:CDS 50.0%
GGAGACGTATGTGAGGTATG+GGG - chr4_1:83976625-83976644 Msa0579310:CDS 50.0%
! GCGTGTGAAGAGGTATGAGA+GGG - chr4_1:83976682-83976701 Msa0579310:CDS 50.0%
CAACCAACACTCTCGTTAGC+AGG + chr4_1:83976725-83976744 None:intergenic 50.0%
!! CTGCTAACGAGAGTGTTGGT+TGG - chr4_1:83976723-83976742 Msa0579310:CDS 50.0%
!! CTAACGAGAGTGTTGGTTGG+AGG - chr4_1:83976726-83976745 Msa0579310:CDS 50.0%
! CTACGGTTGACTAGCTGACA+AGG + chr4_1:83977632-83977651 None:intergenic 50.0%
!! GAGTGATGGTGTTGTGTACC+AGG - chr4_1:83977957-83977976 Msa0579310:intron 50.0%
!! GATGGTGTTGTGTACCAGGT+TGG - chr4_1:83977961-83977980 Msa0579310:intron 50.0%
CTAGAGCTTCAACATCACGG+AGG + chr4_1:83978146-83978165 None:intergenic 50.0%
CTCCGTGATGTTGAAGCTCT+AGG - chr4_1:83978144-83978163 Msa0579310:intron 50.0%
CCGATGTGTGACACAAAAGC+GGG + chr4_1:83978177-83978196 None:intergenic 50.0%
!! CCCGCTTTTGTGTCACACAT+CGG - chr4_1:83978174-83978193 Msa0579310:intron 50.0%
GTTACGCAAGTGGTTATGCG+TGG - chr4_1:83978205-83978224 Msa0579310:intron 50.0%
CAAGTGGTTATGCGTGGTTG+TGG - chr4_1:83978211-83978230 Msa0579310:intron 50.0%
AAAGATGGAGGTGGTGAGTG+CGG - chr4_1:83978354-83978373 Msa0579310:intron 50.0%
GCTGGAGCTATAGTTCCAGT+AGG + chr4_1:83978426-83978445 None:intergenic 50.0%
CTACACTTGTGGAAATGGCG+TGG - chr4_1:83978761-83978780 Msa0579310:CDS 50.0%
CATCTTGTGATTGGCATGGC+AGG - chr4_1:83978816-83978835 Msa0579310:CDS 50.0%
GGCAAGACAAGCAATCCATC+TGG - chr4_1:83978838-83978857 Msa0579310:CDS 50.0%
!!! ACCCGGTCTTGTTTTCCAGA+TGG + chr4_1:83978856-83978875 None:intergenic 50.0%
ATCGAGTCATTGGGATGACC+CGG + chr4_1:83978873-83978892 None:intergenic 50.0%
TTGTATCGTGGGGGTGAGTT+TGG - chr4_1:83978915-83978934 Msa0579310:CDS 50.0%
TGTATCGTGGGGGTGAGTTT+GGG - chr4_1:83978916-83978935 Msa0579310:CDS 50.0%
TGGTGAGGTGCATGACACAA+TGG - chr4_1:83978998-83979017 Msa0579310:CDS 50.0%
TTATCCCAGTATGTCCAGGG+AGG - chr4_1:83979110-83979129 Msa0579310:CDS 50.0%
GTAGAAACACTCCTGGACTC+TGG + chr4_1:83979203-83979222 None:intergenic 50.0%
CGTGTGAGTAGAAACACTCC+TGG + chr4_1:83979210-83979229 None:intergenic 50.0%
GAGTTGTGGGGGTTACGGTT+AGG + chr4_1:83976199-83976218 None:intergenic 55.0%
ACCGTCACACTAAGGTGGTC+CGG - chr4_1:83976236-83976255 Msa0579310:CDS 55.0%
ACCGGACCACCTTAGTGTGA+CGG + chr4_1:83976240-83976259 None:intergenic 55.0%
GAAGTTGTCGTAGGAGGAGG+TGG + chr4_1:83976309-83976328 None:intergenic 55.0%
!! TGGAACGGAGGTTGATGAGG+GGG + chr4_1:83976394-83976413 None:intergenic 55.0%
! GGTTTCCGGTTTGTGGCAAG+GGG + chr4_1:83976487-83976506 None:intergenic 55.0%
! GCGAGAAGGTTTCCGGTTTG+TGG + chr4_1:83976494-83976513 None:intergenic 55.0%
GTATGTGACGAGGGATCCGA+AGG - chr4_1:83976604-83976623 Msa0579310:CDS 55.0%
TCATATGGGAGGTCTTGCGG+TGG - chr4_1:83976658-83976677 Msa0579310:CDS 55.0%
! CGCGTGTGAAGAGGTATGAG+AGG - chr4_1:83976681-83976700 Msa0579310:CDS 55.0%
! GCTCCTGCTAACGAGAGTGT+TGG - chr4_1:83976719-83976738 Msa0579310:CDS 55.0%
!! AGTGTTGGTTGGAGGGATCC+AGG - chr4_1:83976734-83976753 Msa0579310:CDS 55.0%
ATCCTTTGTCCCCTGCTCTC+CGG + chr4_1:83978788-83978807 None:intergenic 55.0%
GGCTACAAAGCAGAAGCTCG+TGG - chr4_1:83978953-83978972 Msa0579310:CDS 55.0%
GCTTCCTCCCTGGACATACT+GGG + chr4_1:83979117-83979136 None:intergenic 55.0%
! GAGGAAGCGTACTGGTGCTT+GGG - chr4_1:83979129-83979148 Msa0579310:CDS 55.0%
! AGGAAGCGTACTGGTGCTTG+GGG - chr4_1:83979130-83979149 Msa0579310:CDS 55.0%
ACTGGTGCTTGGGGCCTTTA+TGG - chr4_1:83979139-83979158 Msa0579310:CDS 55.0%
CTGGTGCTTGGGGCCTTTAT+GGG - chr4_1:83979140-83979159 Msa0579310:CDS 55.0%
GTGGGGGTTACGGTTAGGGA+GGG + chr4_1:83976194-83976213 None:intergenic 60.0%
TGTGGGGGTTACGGTTAGGG+AGG + chr4_1:83976195-83976214 None:intergenic 60.0%
CCTTAGTGTGACGGTGTCCC+CGG + chr4_1:83976231-83976250 None:intergenic 60.0%
GGGACACCGTCACACTAAGG+TGG - chr4_1:83976231-83976250 Msa0579310:CDS 60.0%
TTGTCGTAGGAGGAGGTGGG+TGG + chr4_1:83976305-83976324 None:intergenic 60.0%
TCCACCTGGCAATCCGGTTC+CGG - chr4_1:83976356-83976375 Msa0579310:CDS 60.0%
!! ACGGAGGTTGATGAGGGGGA+GGG + chr4_1:83976390-83976409 None:intergenic 60.0%
!! AACGGAGGTTGATGAGGGGG+AGG + chr4_1:83976391-83976410 None:intergenic 60.0%
GAACATGGCCCCAAAGGGCA+AGG + chr4_1:83978710-83978729 None:intergenic 60.0%
CTTGTGGAAATGGCGTGGGC+CGG - chr4_1:83978766-83978785 Msa0579310:CDS 60.0%
CGCTTCCTCCCTGGACATAC+TGG + chr4_1:83979118-83979137 None:intergenic 60.0%
! TGTCCAGGGAGGAAGCGTAC+TGG - chr4_1:83979121-83979140 Msa0579310:CDS 60.0%
! GGAGGAAGCGTACTGGTGCT+TGG - chr4_1:83979128-83979147 Msa0579310:CDS 60.0%
CCGGGGACACCGTCACACTA+AGG - chr4_1:83976228-83976247 Msa0579310:CDS 65.0%
TGTGCCGGAACCGGATTGCC+AGG + chr4_1:83976363-83976382 None:intergenic 65.0%
! GAGGGAGAGTGTGCCGGAAC+CGG + chr4_1:83976372-83976391 None:intergenic 65.0%
TTGCGGTGGCGCGTGTGAAG+AGG - chr4_1:83976672-83976691 Msa0579310:CDS 65.0%
GCACCAGTACGCTTCCTCCC+TGG + chr4_1:83979127-83979146 None:intergenic 65.0%
GCCGGAACCGGATTGCCAGG+TGG + chr4_1:83976360-83976379 None:intergenic 70.0%
! GAGGGGGAGGGAGAGTGTGC+CGG + chr4_1:83976378-83976397 None:intergenic 70.0%
AATGGCGTGGGCCGGAGAGC+AGG - chr4_1:83978774-83978793 Msa0579310:CDS 70.0%
ATGGCGTGGGCCGGAGAGCA+GGG - chr4_1:83978775-83978794 Msa0579310:CDS 70.0%
GGCCGGAGAGCAGGGGACAA+AGG - chr4_1:83978783-83978802 Msa0579310:CDS 70.0%
TGGCGTGGGCCGGAGAGCAG+GGG - chr4_1:83978776-83978795 Msa0579310:CDS 75.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 83976122 83979250 83976122 ID=Msa0579310;Name=Msa0579310
chr4_1 mRNA 83976122 83979250 83976122 ID=Msa0579310-mRNA-1;Parent=Msa0579310;Name=Msa0579310-mRNA-1;_AED=0.34;_eAED=0.34;_QI=0|0|0|1|1|1|2|0|654
chr4_1 exon 83978558 83979250 83978558 ID=Msa0579310-mRNA-1:exon:30781;Parent=Msa0579310-mRNA-1
chr4_1 exon 83976122 83977393 83976122 ID=Msa0579310-mRNA-1:exon:30780;Parent=Msa0579310-mRNA-1
chr4_1 CDS 83978558 83979250 83978558 ID=Msa0579310-mRNA-1:cds;Parent=Msa0579310-mRNA-1
chr4_1 CDS 83976122 83977393 83976122 ID=Msa0579310-mRNA-1:cds;Parent=Msa0579310-mRNA-1
Gene Sequence

>Msa0579310

ATGATTCCTTGCACAATACTCTTCCTCTATCTTTCAATAATCTTCAACTCCAACCTTGTCCAATCAAAACCCTCCCTAACCGTAACCCCCACAACTCTATCAAAATCCGGGGACACCGTCACACTAAGGTGGTCCGGTATCCAATCACCCTCAGAGCTAGACTTCGTAGCAATCTACTCACCACCCACCTCCTCCTACGACAACTTCATTGGCTACCTCTTCCTCTCTAAATCATCCACCTGGCAATCCGGTTCCGGCACACTCTCCCTCCCCCTCATCAACCTCCGTTCCAACTACATCTTCGGTATCTTCCATTGGACCCAATCCGAAATCAACCCTGATCGTTATGACCATGACAATAACCCCTTGCCACAAACCGGAAACCTTCTCGCCATTTCTGAAGAGGTTTCGTTTGTTTCGGGTAAAGGACCGGAGCAGATTCATCTTGCTTTTGCGGATGAAGAAGATGCTATGCGGGTTATGTATGTGACGAGGGATCCGAAGGAGACGTATGTGAGGTATGGGGAGAGAAAATGTCATATGGGAGGTCTTGCGGTGGCGCGTGTGAAGAGGTATGAGAGGGAGCATATGTGTGATGCTCCTGCTAACGAGAGTGTTGGTTGGAGGGATCCAGGTTATATACATGATGCTTTGATCACTAGTTTAAAGAAAGGAAGAAGATACTACTATAAGGTTGGAAATAAAAATGGAGGTTGGAGTGCAACTCATAGCTTTGTGTCAAGGAATAGTGATTCAAATGAAACAATAGCTTTCATTTTTGGTGACATGGGAACAGCTACACCATATAATACATTTTTGCGTACACAAGAAGAAAGCATATCAACAGTGAAGTGGATCCTCCGTGATGTTGAAGCTCTAGGAAACAAACCCGCTTTTGTGTCACACATCGGAGACATTAGTTACGCAAGTGGTTATGCGTGGTTGTGGGATCATTTTTTCGCACAAATTGAACCTGTTGCAACAAAAGTGGCATACCATGTATGCATTGGAAATCATGAGTATGATTGGCCTTTACAGCCATGGAAACCTAATTGGACTAATTATGGAAAAGATGGAGGTGGTGAGTGCGGTGTACCCTATAGTTTAAGGTTCAACATGCCTGGAAATTCGTCAGAACCTACTGGAACTATAGCTCCAGCAACTAGAAATCTTTATTACTCATTTGATATGGGTGTAGTACATTTTGTTTATATATCAACAGAGACCAATTTTCTTCTTGGGAGCAACCAGTATAACTTCTTGAAGCATGATTTGGAATCAGTTGACAGGAACAAGACTCCTTTTGTAGTAGTTCAAGGGCACAGACCCATGTACACAACAATCAATGGAACCAAGGATGTTGCGTTGAGAGAAAAGATGCTTGAGCACTTGGAACCTCTATTGGTGAACAACAATGTGAACCTTGCCCTTTGGGGCCATGTTCATAGATACGAGAGATTTTGTCCACTGAATAACTACACTTGTGGAAATGGCGTGGGCCGGAGAGCAGGGGACAAAGGATATACCGTTCATCTTGTGATTGGCATGGCAGGGCAAGACAAGCAATCCATCTGGAAAACAAGACCGGGTCATCCCAATGACTCGATCTTTCCTCAACCAAAACAGTCTTTGTATCGTGGGGGTGAGTTTGGGTACATTAGACTAGTGGCTACAAAGCAGAAGCTCGTGGTTTCTTATGTTGGAAATCATGATGGTGAGGTGCATGACACAATGGAAATTTTGGCATCTGGAGAACTTTTCAATGGTAATGTTAATGTTGATATTGGTAATGCTAAACCTAAAGGTTTGATTGAAGAAGCTACATTATCCCAGTATGTCCAGGGAGGAAGCGTACTGGTGCTTGGGGCCTTTATGGGTTACATTCTCGGTTGGGTTATACATGCAAGGAAAAAACCAGAGTCCAGGAGTGTTTCTACTCACACGAAGATTGAGAAACATGATTAG
Protein sequence

>Msa0579310

MIPCTILFLYLSIIFNSNLVQSKPSLTVTPTTLSKSGDTVTLRWSGIQSPSELDFVAIYSPPTSSYDNFIGYLFLSKSSTWQSGSGTLSLPLINLRSNYIFGIFHWTQSEINPDRYDHDNNPLPQTGNLLAISEEVSFVSGKGPEQIHLAFADEEDAMRVMYVTRDPKETYVRYGERKCHMGGLAVARVKRYEREHMCDAPANESVGWRDPGYIHDALITSLKKGRRYYYKVGNKNGGWSATHSFVSRNSDSNETIAFIFGDMGTATPYNTFLRTQEESISTVKWILRDVEALGNKPAFVSHIGDISYASGYAWLWDHFFAQIEPVATKVAYHVCIGNHEYDWPLQPWKPNWTNYGKDGGGECGVPYSLRFNMPGNSSEPTGTIAPATRNLYYSFDMGVVHFVYISTETNFLLGSNQYNFLKHDLESVDRNKTPFVVVQGHRPMYTTINGTKDVALREKMLEHLEPLLVNNNVNLALWGHVHRYERFCPLNNYTCGNGVGRRAGDKGYTVHLVIGMAGQDKQSIWKTRPGHPNDSIFPQPKQSLYRGGEFGYIRLVATKQKLVVSYVGNHDGEVHDTMEILASGELFNGNVNVDIGNAKPKGLIEEATLSQYVQGGSVLVLGAFMGYILGWVIHARKKPESRSVSTHTKIEKHD*