AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0651020

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0651020 MtrunA17_Chr4g0043351 86.452 155 18 1 16 167 8 162 4.30e-95 272
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 74 sgRNAs with CRISPR-Local

Find 180 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAGATCAAGCTAACCTTTCT+TGG 0.224166 4_3:-58301880 Msa0651020:three_prime_UTR
GATGGAATTGAACCGCTTTC+AGG 0.230161 4_3:-58302423 Msa0651020:CDS
ATATTGCTTTAGAATTAATT+TGG 0.230650 4_3:-58301698 Msa0651020:three_prime_UTR
TTCAAAGTTCAAATTCTTAG+AGG 0.303256 4_3:-58302018 Msa0651020:CDS
AGTTGGGCCACGACTTATAA+TGG 0.308961 4_3:-58302162 Msa0651020:CDS
ACGGGGACTAAGATGTGTTT+TGG 0.312922 4_3:+58302374 None:intergenic
TTCCGACTATTCTCAAATAT+CGG 0.320685 4_3:-58302191 Msa0651020:CDS
TTTCTTGGCTTGACCTTTGT+GGG 0.323305 4_3:-58301865 Msa0651020:three_prime_UTR
AAGCAATATGTTTCTCTGAT+TGG 0.332487 4_3:+58301712 None:intergenic
GCATCCCTCTCACCGGTTCC+AGG 0.356774 4_3:+58301964 None:intergenic
CTTTCTTGGCTTGACCTTTG+TGG 0.363705 4_3:-58301866 Msa0651020:three_prime_UTR
AGAGCATAGACTTTGGTGTT+AGG 0.364464 4_3:-58301800 Msa0651020:three_prime_UTR
GACCTTTGTGGGATTGTTCA+AGG 0.364760 4_3:-58301854 Msa0651020:three_prime_UTR
TGAAATTACCAATCCATAAC+GGG 0.384987 4_3:+58302356 None:intergenic
GAGAATAGTCGGAACCGATT+TGG 0.387243 4_3:+58302200 None:intergenic
ACACATCTTAGTCCCCGTTA+TGG 0.404304 4_3:-58302369 Msa0651020:CDS
GAAGATAATTATTTGAAGCT+TGG 0.405476 4_3:-58302090 Msa0651020:CDS
TCCGACTATTCTCAAATATC+GGG 0.408506 4_3:-58302190 Msa0651020:CDS
GCCAACGCTATCAAACAGTT+TGG 0.414501 4_3:+58302128 None:intergenic
AACTGTTTGATAGCGTTGGC+TGG 0.415821 4_3:-58302125 Msa0651020:CDS
GAAAGGACCACATGAAAGTA+TGG 0.437117 4_3:+58302396 None:intergenic
TCGGTGATGGTGGTTGGTGG+TGG 0.442258 4_3:+58302782 None:intergenic
ATACAGAGGGAGAGTGATGA+TGG 0.448288 4_3:+58302819 None:intergenic
CTTTACGAATTTCCTGGAAC+CGG 0.450094 4_3:-58301976 Msa0651020:CDS
CAAATATCGGGGTAAGAGTT+GGG 0.464634 4_3:-58302178 Msa0651020:CDS
GATTACTAATCTATGACAAA+TGG 0.467072 4_3:+58301940 None:intergenic
TCAAATATCGGGGTAAGAGT+TGG 0.468409 4_3:-58302179 Msa0651020:CDS
TGAAGAACTTCCTTCCAAAT+CGG 0.469161 4_3:-58302214 Msa0651020:CDS
GTTGGGCCACGACTTATAAT+GGG 0.482324 4_3:-58302161 Msa0651020:CDS
TAAAGGTCGCCGGAAGGATG+TGG 0.497755 4_3:+58302743 None:intergenic
ATGAAATTACCAATCCATAA+CGG 0.499697 4_3:+58302355 None:intergenic
TTGTCGGTGATGGTGGTTGG+TGG 0.500922 4_3:+58302779 None:intergenic
ATGTCGTCTTCAAAATCAGC+TGG 0.502794 4_3:-58302608 Msa0651020:five_prime_UTR
ACCAAACTGTTTGATAGCGT+TGG 0.508522 4_3:-58302129 Msa0651020:CDS
CACAAAGGTCAAGCCAAGAA+AGG 0.514465 4_3:+58301867 None:intergenic
CCCCGATATTTGAGAATAGT+CGG 0.515602 4_3:+58302189 None:intergenic
TCTTAGTCCCCGTTATGGAT+TGG 0.515821 4_3:-58302364 Msa0651020:intron
CGAACAGATCCACATCCTTC+CGG 0.519644 4_3:-58302752 Msa0651020:five_prime_UTR
CGGAAGGATGTGGATCTGTT+CGG 0.521075 4_3:+58302753 None:intergenic
ACCTTCGACAAATATGCAAA+CGG 0.530039 4_3:-58302445 Msa0651020:CDS
TTGCTTTAGAATTAATTTGG+TGG 0.530220 4_3:-58301695 Msa0651020:three_prime_UTR
GTGTACATGAAAGTTAATCT+TGG 0.531460 4_3:+58302549 None:intergenic
TAATGTGATATGAAATACTA+AGG 0.533146 4_3:+58302572 None:intergenic
GGAATAGTGAAGTTATGTAC+TGG 0.534448 4_3:-58301779 Msa0651020:three_prime_UTR
TGTTCGGAAGTTGTCGGTGA+TGG 0.534909 4_3:+58302769 None:intergenic
ACTGTTTGATAGCGTTGGCT+GGG 0.539366 4_3:-58302124 Msa0651020:CDS
GAAGGTGATGATAACGAGGC+TGG 0.539673 4_3:+58302462 None:intergenic
TCCGTTTGCATATTTGTCGA+AGG 0.549182 4_3:+58302444 None:intergenic
GTTCTGCCCATTATAAGTCG+TGG 0.557426 4_3:+58302155 None:intergenic
GGTTGGTGGTGGAAAAGAAA+AGG 0.558273 4_3:+58302793 None:intergenic
AAGTTGTCGGTGATGGTGGT+TGG 0.562351 4_3:+58302776 None:intergenic
AAAGAAAAGGAAAATACAGA+GGG 0.564283 4_3:+58302806 None:intergenic
TCTTCAAAATCAGCTGGAAG+AGG 0.564849 4_3:-58302602 Msa0651020:five_prime_UTR
AAATGGTGCATCCCTCTCAC+CGG 0.578086 4_3:+58301957 None:intergenic
AGGAGTGCCATACTTTCATG+TGG 0.578309 4_3:-58302403 Msa0651020:CDS
TCGACAAATATGCAAACGGA+TGG 0.583245 4_3:-58302441 Msa0651020:CDS
GAGTAGCAGTCAAGTTGCTG+TGG 0.583739 4_3:-58302490 Msa0651020:CDS
TCTTCACATATGCTGCTGCT+CGG 0.585483 4_3:+58302231 None:intergenic
ATTTCCTGGAACCGGTGAGA+GGG 0.587927 4_3:-58301968 Msa0651020:CDS
AGTAGCAGTCAAGTTGCTGT+GGG 0.603556 4_3:-58302489 Msa0651020:CDS
GTAGGTTAGAGCATAGACTT+TGG 0.607501 4_3:-58301807 Msa0651020:three_prime_UTR
TGGATCTGTTCGGAAGTTGT+CGG 0.611775 4_3:+58302763 None:intergenic
TTAACTTTCATGTACACGGT+CGG 0.616082 4_3:-58302543 Msa0651020:exon
TCGGAAGTTGTCGGTGATGG+TGG 0.619536 4_3:+58302772 None:intergenic
TTTAAGTAAAGGTCGCCGGA+AGG 0.626587 4_3:+58302737 None:intergenic
AAAAGAAAAGGAAAATACAG+AGG 0.630802 4_3:+58302805 None:intergenic
AATTTCCTGGAACCGGTGAG+AGG 0.633829 4_3:-58301969 Msa0651020:CDS
ATAGTCGGAACCGATTTGGA+AGG 0.643344 4_3:+58302204 None:intergenic
AAGATTAACTTTCATGTACA+CGG 0.643809 4_3:-58302547 Msa0651020:exon
AAGTATGGCACTCCTGAAAG+CGG 0.678549 4_3:+58302411 None:intergenic
CCGACTATTCTCAAATATCG+GGG 0.681942 4_3:-58302189 Msa0651020:CDS
AACCTTGAACAATCCCACAA+AGG 0.707964 4_3:+58301852 None:intergenic
GAAATTACCAATCCATAACG+GGG 0.716232 4_3:+58302357 None:intergenic
TGTCGAAGGTGATGATAACG+AGG 0.728967 4_3:+58302458 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATGTAATTTTTTTTAAGTAA+AGG + chr4_3:58301787-58301806 None:intergenic 10.0%
!!! ATGTAATTTTTTTTAAGTAA+AGG + chr4_3:58301787-58301806 None:intergenic 10.0%
!!! TATCAAATTAGATTTTCAAA+AGG - chr4_3:58301839-58301858 Msa0651020:three_prime_UTR 15.0%
!!! ATATTGCTTTAGAATTAATT+TGG - chr4_3:58302812-58302831 Msa0651020:five_prime_UTR 15.0%
!!! TATCAAATTAGATTTTCAAA+AGG - chr4_3:58301839-58301858 Msa0651020:three_prime_UTR 15.0%
!!! ATATTGCTTTAGAATTAATT+TGG - chr4_3:58302812-58302831 Msa0651020:five_prime_UTR 15.0%
!! TAATGTGATATGAAATACTA+AGG + chr4_3:58301941-58301960 None:intergenic 20.0%
!! TTATAGATCAAACTAAATGA+AGG - chr4_3:58302195-58302214 Msa0651020:CDS 20.0%
!! TAAATCTTGTATTGTATTGT+TGG - chr4_3:58302246-58302265 Msa0651020:intron 20.0%
!! TAATGTGATATGAAATACTA+AGG + chr4_3:58301941-58301960 None:intergenic 20.0%
!! TTATAGATCAAACTAAATGA+AGG - chr4_3:58302195-58302214 Msa0651020:CDS 20.0%
!! TAAATCTTGTATTGTATTGT+TGG - chr4_3:58302246-58302265 Msa0651020:intron 20.0%
! AAAGAAAAGGAAAATACAGA+GGG + chr4_3:58301707-58301726 None:intergenic 25.0%
! AAAAGAAAAGGAAAATACAG+AGG + chr4_3:58301708-58301727 None:intergenic 25.0%
! AAGATTAACTTTCATGTACA+CGG - chr4_3:58301963-58301982 Msa0651020:CDS 25.0%
! ATGAAATTACCAATCCATAA+CGG + chr4_3:58302158-58302177 None:intergenic 25.0%
!! ATCAAACTAAATGAAGGTTT+TGG - chr4_3:58302201-58302220 Msa0651020:CDS 25.0%
! TATTGTATTGTTGGCATATA+GGG - chr4_3:58302255-58302274 Msa0651020:intron 25.0%
!! GAAGATAATTATTTGAAGCT+TGG - chr4_3:58302420-58302439 Msa0651020:CDS 25.0%
! TTCAAAGTTCAAATTCTTAG+AGG - chr4_3:58302492-58302511 Msa0651020:CDS 25.0%
! GATTACTAATCTATGACAAA+TGG + chr4_3:58302573-58302592 None:intergenic 25.0%
!! GATCTAGTTTCAAAAAGAAA+AGG + chr4_3:58302616-58302635 None:intergenic 25.0%
!!! TTGCTTTAGAATTAATTTGG+TGG - chr4_3:58302815-58302834 Msa0651020:five_prime_UTR 25.0%
! AAAGAAAAGGAAAATACAGA+GGG + chr4_3:58301707-58301726 None:intergenic 25.0%
! AAAAGAAAAGGAAAATACAG+AGG + chr4_3:58301708-58301727 None:intergenic 25.0%
! AAGATTAACTTTCATGTACA+CGG - chr4_3:58301963-58301982 Msa0651020:CDS 25.0%
! ATGAAATTACCAATCCATAA+CGG + chr4_3:58302158-58302177 None:intergenic 25.0%
!! ATCAAACTAAATGAAGGTTT+TGG - chr4_3:58302201-58302220 Msa0651020:CDS 25.0%
! TATTGTATTGTTGGCATATA+GGG - chr4_3:58302255-58302274 Msa0651020:intron 25.0%
!! GAAGATAATTATTTGAAGCT+TGG - chr4_3:58302420-58302439 Msa0651020:CDS 25.0%
! TTCAAAGTTCAAATTCTTAG+AGG - chr4_3:58302492-58302511 Msa0651020:CDS 25.0%
! GATTACTAATCTATGACAAA+TGG + chr4_3:58302573-58302592 None:intergenic 25.0%
!! GATCTAGTTTCAAAAAGAAA+AGG + chr4_3:58302616-58302635 None:intergenic 25.0%
!!! TTGCTTTAGAATTAATTTGG+TGG - chr4_3:58302815-58302834 Msa0651020:five_prime_UTR 25.0%
!!! TTTTTTTAAGTAAAGGTCGC+CGG + chr4_3:58301780-58301799 None:intergenic 30.0%
GTGTACATGAAAGTTAATCT+TGG + chr4_3:58301964-58301983 None:intergenic 30.0%
TGAAATTACCAATCCATAAC+GGG + chr4_3:58302157-58302176 None:intergenic 30.0%
!!! ACTAAATGAAGGTTTTGGAA+AGG - chr4_3:58302206-58302225 Msa0651020:CDS 30.0%
GTATTGTATTGTTGGCATAT+AGG - chr4_3:58302254-58302273 Msa0651020:intron 30.0%
ATTGTATTGTTGGCATATAG+GGG - chr4_3:58302256-58302275 Msa0651020:intron 30.0%
TTCCGACTATTCTCAAATAT+CGG - chr4_3:58302319-58302338 Msa0651020:intron 30.0%
GGAAATTCGTAAAGCAAAAA+TGG + chr4_3:58302528-58302547 None:intergenic 30.0%
! TTTTTGCTTTACGAATTTCC+TGG - chr4_3:58302528-58302547 Msa0651020:CDS 30.0%
!!! GATACTTTTGTTTTGTGAGT+AGG - chr4_3:58302685-58302704 Msa0651020:five_prime_UTR 30.0%
AAGCAATATGTTTCTCTGAT+TGG + chr4_3:58302801-58302820 None:intergenic 30.0%
!!! TTTTTTTAAGTAAAGGTCGC+CGG + chr4_3:58301780-58301799 None:intergenic 30.0%
GTGTACATGAAAGTTAATCT+TGG + chr4_3:58301964-58301983 None:intergenic 30.0%
TGAAATTACCAATCCATAAC+GGG + chr4_3:58302157-58302176 None:intergenic 30.0%
!!! ACTAAATGAAGGTTTTGGAA+AGG - chr4_3:58302206-58302225 Msa0651020:CDS 30.0%
GTATTGTATTGTTGGCATAT+AGG - chr4_3:58302254-58302273 Msa0651020:intron 30.0%
ATTGTATTGTTGGCATATAG+GGG - chr4_3:58302256-58302275 Msa0651020:intron 30.0%
TTCCGACTATTCTCAAATAT+CGG - chr4_3:58302319-58302338 Msa0651020:intron 30.0%
GGAAATTCGTAAAGCAAAAA+TGG + chr4_3:58302528-58302547 None:intergenic 30.0%
! TTTTTGCTTTACGAATTTCC+TGG - chr4_3:58302528-58302547 Msa0651020:CDS 30.0%
!!! GATACTTTTGTTTTGTGAGT+AGG - chr4_3:58302685-58302704 Msa0651020:five_prime_UTR 30.0%
AAGCAATATGTTTCTCTGAT+TGG + chr4_3:58302801-58302820 None:intergenic 30.0%
TTAACTTTCATGTACACGGT+CGG - chr4_3:58301967-58301986 Msa0651020:CDS 35.0%
ACCTTCGACAAATATGCAAA+CGG - chr4_3:58302065-58302084 Msa0651020:CDS 35.0%
!!! GACTAAGATGTGTTTTGGAA+AGG + chr4_3:58302134-58302153 None:intergenic 35.0%
GAAATTACCAATCCATAACG+GGG + chr4_3:58302156-58302175 None:intergenic 35.0%
TGAAGAACTTCCTTCCAAAT+CGG - chr4_3:58302296-58302315 Msa0651020:intron 35.0%
TCCGACTATTCTCAAATATC+GGG - chr4_3:58302320-58302339 Msa0651020:intron 35.0%
TAGATCAAGCTAACCTTTCT+TGG - chr4_3:58302630-58302649 Msa0651020:five_prime_UTR 35.0%
GGAATAGTGAAGTTATGTAC+TGG - chr4_3:58302731-58302750 Msa0651020:five_prime_UTR 35.0%
TTAACTTTCATGTACACGGT+CGG - chr4_3:58301967-58301986 Msa0651020:CDS 35.0%
ACCTTCGACAAATATGCAAA+CGG - chr4_3:58302065-58302084 Msa0651020:CDS 35.0%
!!! GACTAAGATGTGTTTTGGAA+AGG + chr4_3:58302134-58302153 None:intergenic 35.0%
GAAATTACCAATCCATAACG+GGG + chr4_3:58302156-58302175 None:intergenic 35.0%
TGAAGAACTTCCTTCCAAAT+CGG - chr4_3:58302296-58302315 Msa0651020:intron 35.0%
TCCGACTATTCTCAAATATC+GGG - chr4_3:58302320-58302339 Msa0651020:intron 35.0%
TAGATCAAGCTAACCTTTCT+TGG - chr4_3:58302630-58302649 Msa0651020:five_prime_UTR 35.0%
GGAATAGTGAAGTTATGTAC+TGG - chr4_3:58302731-58302750 Msa0651020:five_prime_UTR 35.0%
ATGTCGTCTTCAAAATCAGC+TGG - chr4_3:58301902-58301921 Msa0651020:three_prime_UTR 40.0%
TCTTCAAAATCAGCTGGAAG+AGG - chr4_3:58301908-58301927 Msa0651020:three_prime_UTR 40.0%
TCCGTTTGCATATTTGTCGA+AGG + chr4_3:58302069-58302088 None:intergenic 40.0%
TCGACAAATATGCAAACGGA+TGG - chr4_3:58302069-58302088 Msa0651020:CDS 40.0%
GAAAGGACCACATGAAAGTA+TGG + chr4_3:58302117-58302136 None:intergenic 40.0%
CCCCGATATTTGAGAATAGT+CGG + chr4_3:58302324-58302343 None:intergenic 40.0%
CCGACTATTCTCAAATATCG+GGG - chr4_3:58302321-58302340 Msa0651020:intron 40.0%
TCAAATATCGGGGTAAGAGT+TGG - chr4_3:58302331-58302350 Msa0651020:intron 40.0%
CAAATATCGGGGTAAGAGTT+GGG - chr4_3:58302332-58302351 Msa0651020:intron 40.0%
!! ACCAAACTGTTTGATAGCGT+TGG - chr4_3:58302381-58302400 Msa0651020:CDS 40.0%
GCTCATGAAAAACAGTGAAG+AGG - chr4_3:58302461-58302480 Msa0651020:CDS 40.0%
CTTTACGAATTTCCTGGAAC+CGG - chr4_3:58302534-58302553 Msa0651020:CDS 40.0%
! TTTCTTGGCTTGACCTTTGT+GGG - chr4_3:58302645-58302664 Msa0651020:five_prime_UTR 40.0%
AACCTTGAACAATCCCACAA+AGG + chr4_3:58302661-58302680 None:intergenic 40.0%
GTAGGTTAGAGCATAGACTT+TGG - chr4_3:58302703-58302722 Msa0651020:five_prime_UTR 40.0%
!! AGAGCATAGACTTTGGTGTT+AGG - chr4_3:58302710-58302729 Msa0651020:five_prime_UTR 40.0%
ATGTCGTCTTCAAAATCAGC+TGG - chr4_3:58301902-58301921 Msa0651020:three_prime_UTR 40.0%
TCTTCAAAATCAGCTGGAAG+AGG - chr4_3:58301908-58301927 Msa0651020:three_prime_UTR 40.0%
TCCGTTTGCATATTTGTCGA+AGG + chr4_3:58302069-58302088 None:intergenic 40.0%
TCGACAAATATGCAAACGGA+TGG - chr4_3:58302069-58302088 Msa0651020:CDS 40.0%
GAAAGGACCACATGAAAGTA+TGG + chr4_3:58302117-58302136 None:intergenic 40.0%
CCCCGATATTTGAGAATAGT+CGG + chr4_3:58302324-58302343 None:intergenic 40.0%
CCGACTATTCTCAAATATCG+GGG - chr4_3:58302321-58302340 Msa0651020:intron 40.0%
TCAAATATCGGGGTAAGAGT+TGG - chr4_3:58302331-58302350 Msa0651020:intron 40.0%
CAAATATCGGGGTAAGAGTT+GGG - chr4_3:58302332-58302351 Msa0651020:intron 40.0%
!! ACCAAACTGTTTGATAGCGT+TGG - chr4_3:58302381-58302400 Msa0651020:CDS 40.0%
GCTCATGAAAAACAGTGAAG+AGG - chr4_3:58302461-58302480 Msa0651020:CDS 40.0%
CTTTACGAATTTCCTGGAAC+CGG - chr4_3:58302534-58302553 Msa0651020:CDS 40.0%
! TTTCTTGGCTTGACCTTTGT+GGG - chr4_3:58302645-58302664 Msa0651020:five_prime_UTR 40.0%
AACCTTGAACAATCCCACAA+AGG + chr4_3:58302661-58302680 None:intergenic 40.0%
GTAGGTTAGAGCATAGACTT+TGG - chr4_3:58302703-58302722 Msa0651020:five_prime_UTR 40.0%
!! AGAGCATAGACTTTGGTGTT+AGG - chr4_3:58302710-58302729 Msa0651020:five_prime_UTR 40.0%
ATACAGAGGGAGAGTGATGA+TGG + chr4_3:58301694-58301713 None:intergenic 45.0%
!! GGTTGGTGGTGGAAAAGAAA+AGG + chr4_3:58301720-58301739 None:intergenic 45.0%
TGGATCTGTTCGGAAGTTGT+CGG + chr4_3:58301750-58301769 None:intergenic 45.0%
TTTAAGTAAAGGTCGCCGGA+AGG + chr4_3:58301776-58301795 None:intergenic 45.0%
! AGTAGCAGTCAAGTTGCTGT+GGG - chr4_3:58302021-58302040 Msa0651020:CDS 45.0%
! TGTCGAAGGTGATGATAACG+AGG + chr4_3:58302055-58302074 None:intergenic 45.0%
GATGGAATTGAACCGCTTTC+AGG - chr4_3:58302087-58302106 Msa0651020:CDS 45.0%
AAGTATGGCACTCCTGAAAG+CGG + chr4_3:58302102-58302121 None:intergenic 45.0%
!! AGGAGTGCCATACTTTCATG+TGG - chr4_3:58302107-58302126 Msa0651020:CDS 45.0%
! ACGGGGACTAAGATGTGTTT+TGG + chr4_3:58302139-58302158 None:intergenic 45.0%
ACACATCTTAGTCCCCGTTA+TGG - chr4_3:58302141-58302160 Msa0651020:CDS 45.0%
TCTTAGTCCCCGTTATGGAT+TGG - chr4_3:58302146-58302165 Msa0651020:CDS 45.0%
TCTTCACATATGCTGCTGCT+CGG + chr4_3:58302282-58302301 None:intergenic 45.0%
!! ATAGTCGGAACCGATTTGGA+AGG + chr4_3:58302309-58302328 None:intergenic 45.0%
GAGAATAGTCGGAACCGATT+TGG + chr4_3:58302313-58302332 None:intergenic 45.0%
AGTTGGGCCACGACTTATAA+TGG - chr4_3:58302348-58302367 Msa0651020:intron 45.0%
GTTGGGCCACGACTTATAAT+GGG - chr4_3:58302349-58302368 Msa0651020:intron 45.0%
GTTCTGCCCATTATAAGTCG+TGG + chr4_3:58302358-58302377 None:intergenic 45.0%
GCCAACGCTATCAAACAGTT+TGG + chr4_3:58302385-58302404 None:intergenic 45.0%
!! AACTGTTTGATAGCGTTGGC+TGG - chr4_3:58302385-58302404 Msa0651020:CDS 45.0%
!! ACTGTTTGATAGCGTTGGCT+GGG - chr4_3:58302386-58302405 Msa0651020:CDS 45.0%
CACAAAGGTCAAGCCAAGAA+AGG + chr4_3:58302646-58302665 None:intergenic 45.0%
! CTTTCTTGGCTTGACCTTTG+TGG - chr4_3:58302644-58302663 Msa0651020:five_prime_UTR 45.0%
GACCTTTGTGGGATTGTTCA+AGG - chr4_3:58302656-58302675 Msa0651020:five_prime_UTR 45.0%
ATACAGAGGGAGAGTGATGA+TGG + chr4_3:58301694-58301713 None:intergenic 45.0%
!! GGTTGGTGGTGGAAAAGAAA+AGG + chr4_3:58301720-58301739 None:intergenic 45.0%
TGGATCTGTTCGGAAGTTGT+CGG + chr4_3:58301750-58301769 None:intergenic 45.0%
TTTAAGTAAAGGTCGCCGGA+AGG + chr4_3:58301776-58301795 None:intergenic 45.0%
! AGTAGCAGTCAAGTTGCTGT+GGG - chr4_3:58302021-58302040 Msa0651020:CDS 45.0%
! TGTCGAAGGTGATGATAACG+AGG + chr4_3:58302055-58302074 None:intergenic 45.0%
GATGGAATTGAACCGCTTTC+AGG - chr4_3:58302087-58302106 Msa0651020:CDS 45.0%
AAGTATGGCACTCCTGAAAG+CGG + chr4_3:58302102-58302121 None:intergenic 45.0%
!! AGGAGTGCCATACTTTCATG+TGG - chr4_3:58302107-58302126 Msa0651020:CDS 45.0%
! ACGGGGACTAAGATGTGTTT+TGG + chr4_3:58302139-58302158 None:intergenic 45.0%
ACACATCTTAGTCCCCGTTA+TGG - chr4_3:58302141-58302160 Msa0651020:CDS 45.0%
TCTTAGTCCCCGTTATGGAT+TGG - chr4_3:58302146-58302165 Msa0651020:CDS 45.0%
TCTTCACATATGCTGCTGCT+CGG + chr4_3:58302282-58302301 None:intergenic 45.0%
!! ATAGTCGGAACCGATTTGGA+AGG + chr4_3:58302309-58302328 None:intergenic 45.0%
GAGAATAGTCGGAACCGATT+TGG + chr4_3:58302313-58302332 None:intergenic 45.0%
AGTTGGGCCACGACTTATAA+TGG - chr4_3:58302348-58302367 Msa0651020:intron 45.0%
GTTGGGCCACGACTTATAAT+GGG - chr4_3:58302349-58302368 Msa0651020:intron 45.0%
GTTCTGCCCATTATAAGTCG+TGG + chr4_3:58302358-58302377 None:intergenic 45.0%
GCCAACGCTATCAAACAGTT+TGG + chr4_3:58302385-58302404 None:intergenic 45.0%
!! AACTGTTTGATAGCGTTGGC+TGG - chr4_3:58302385-58302404 Msa0651020:CDS 45.0%
!! ACTGTTTGATAGCGTTGGCT+GGG - chr4_3:58302386-58302405 Msa0651020:CDS 45.0%
CACAAAGGTCAAGCCAAGAA+AGG + chr4_3:58302646-58302665 None:intergenic 45.0%
! CTTTCTTGGCTTGACCTTTG+TGG - chr4_3:58302644-58302663 Msa0651020:five_prime_UTR 45.0%
GACCTTTGTGGGATTGTTCA+AGG - chr4_3:58302656-58302675 Msa0651020:five_prime_UTR 45.0%
!! AAGTTGTCGGTGATGGTGGT+TGG + chr4_3:58301737-58301756 None:intergenic 50.0%
TGTTCGGAAGTTGTCGGTGA+TGG + chr4_3:58301744-58301763 None:intergenic 50.0%
CGGAAGGATGTGGATCTGTT+CGG + chr4_3:58301760-58301779 None:intergenic 50.0%
CGAACAGATCCACATCCTTC+CGG - chr4_3:58301758-58301777 Msa0651020:three_prime_UTR 50.0%
! GAGTAGCAGTCAAGTTGCTG+TGG - chr4_3:58302020-58302039 Msa0651020:CDS 50.0%
! GAAGGTGATGATAACGAGGC+TGG + chr4_3:58302051-58302070 None:intergenic 50.0%
AATTTCCTGGAACCGGTGAG+AGG - chr4_3:58302541-58302560 Msa0651020:exon 50.0%
ATTTCCTGGAACCGGTGAGA+GGG - chr4_3:58302542-58302561 Msa0651020:exon 50.0%
AAATGGTGCATCCCTCTCAC+CGG + chr4_3:58302556-58302575 None:intergenic 50.0%
!! AAGTTGTCGGTGATGGTGGT+TGG + chr4_3:58301737-58301756 None:intergenic 50.0%
TGTTCGGAAGTTGTCGGTGA+TGG + chr4_3:58301744-58301763 None:intergenic 50.0%
CGGAAGGATGTGGATCTGTT+CGG + chr4_3:58301760-58301779 None:intergenic 50.0%
CGAACAGATCCACATCCTTC+CGG - chr4_3:58301758-58301777 Msa0651020:three_prime_UTR 50.0%
! GAGTAGCAGTCAAGTTGCTG+TGG - chr4_3:58302020-58302039 Msa0651020:CDS 50.0%
! GAAGGTGATGATAACGAGGC+TGG + chr4_3:58302051-58302070 None:intergenic 50.0%
AATTTCCTGGAACCGGTGAG+AGG - chr4_3:58302541-58302560 Msa0651020:exon 50.0%
ATTTCCTGGAACCGGTGAGA+GGG - chr4_3:58302542-58302561 Msa0651020:exon 50.0%
AAATGGTGCATCCCTCTCAC+CGG + chr4_3:58302556-58302575 None:intergenic 50.0%
!! TTGTCGGTGATGGTGGTTGG+TGG + chr4_3:58301734-58301753 None:intergenic 55.0%
! TCGGAAGTTGTCGGTGATGG+TGG + chr4_3:58301741-58301760 None:intergenic 55.0%
TAAAGGTCGCCGGAAGGATG+TGG + chr4_3:58301770-58301789 None:intergenic 55.0%
!! TTGTCGGTGATGGTGGTTGG+TGG + chr4_3:58301734-58301753 None:intergenic 55.0%
! TCGGAAGTTGTCGGTGATGG+TGG + chr4_3:58301741-58301760 None:intergenic 55.0%
TAAAGGTCGCCGGAAGGATG+TGG + chr4_3:58301770-58301789 None:intergenic 55.0%
!! TCGGTGATGGTGGTTGGTGG+TGG + chr4_3:58301731-58301750 None:intergenic 60.0%
!! TCGGTGATGGTGGTTGGTGG+TGG + chr4_3:58301731-58301750 None:intergenic 60.0%
!! GCATCCCTCTCACCGGTTCC+AGG + chr4_3:58302549-58302568 None:intergenic 65.0%
!! GCATCCCTCTCACCGGTTCC+AGG + chr4_3:58302549-58302568 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 58301679 58302853 58301679 ID=Msa0651020;Name=Msa0651020
chr4_3 mRNA 58301679 58302853 58301679 ID=Msa0651020-mRNA-1;Parent=Msa0651020;Name=Msa0651020-mRNA-1;_AED=0.05;_eAED=0.05;_QI=297|1|1|1|1|1|2|266|167
chr4_3 exon 58301679 58302256 58301679 ID=Msa0651020-mRNA-1:exon:15447;Parent=Msa0651020-mRNA-1
chr4_3 exon 58302365 58302853 58302365 ID=Msa0651020-mRNA-1:exon:15446;Parent=Msa0651020-mRNA-1
chr4_3 five_prime_UTR 58302557 58302853 58302557 ID=Msa0651020-mRNA-1:five_prime_utr;Parent=Msa0651020-mRNA-1
chr4_3 CDS 58302365 58302556 58302365 ID=Msa0651020-mRNA-1:cds;Parent=Msa0651020-mRNA-1
chr4_3 CDS 58301945 58302256 58301945 ID=Msa0651020-mRNA-1:cds;Parent=Msa0651020-mRNA-1
chr4_3 three_prime_UTR 58301679 58301944 58301679 ID=Msa0651020-mRNA-1:three_prime_utr;Parent=Msa0651020-mRNA-1
Gene Sequence

>Msa0651020

ATGTACACGGTCGGTCTATCACTAATTTCACATTTATTTTCAGTGAGTAGCAGTCAAGTTGCTGTGGGAAATCCAGCCTCGTTATCATCACCTTCGACAAATATGCAAACGGATGGAATTGAACCGCTTTCAGGAGTGCCATACTTTCATGTGGTCCTTTCCAAAACACATCTTAGTCCCCGTTATGGATTGGGGCCGAGCAGCAGCATATGTGAAGAACTTCCTTCCAAATCGGTTCCGACTATTCTCAAATATCGGGGTAAGAGTTGGGCCACGACTTATAATGGGCAGAACAAAACCAAACTGTTTGATAGCGTTGGCTGGGAAAAATTTGCTGAAGATAATTATTTGAAGCTTGGAGACGCTTGTGTTTTTGAGCTCATGAAAAACAGTGAAGAGGAAATCGTCTTCAAAGTTCAAATTCTTAGAGGTGAAGAAGAGCCATTTTTGCTTTACGAATTTCCTGGAACCGGTGAGAGGGATGCACCATTTGTCATAGATTAG
Protein sequence

>Msa0651020

MYTVGLSLISHLFSVSSSQVAVGNPASLSSPSTNMQTDGIEPLSGVPYFHVVLSKTHLSPRYGLGPSSSICEELPSKSVPTILKYRGKSWATTYNGQNKTKLFDSVGWEKFAEDNYLKLGDACVFELMKNSEEEIVFKVQILRGEEEPFLLYEFPGTGERDAPFVID*