AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0693070

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0693070 MtrunA17_Chr4g0043351 87.097 155 17 1 16 167 8 162 2.40e-96 275
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 73 sgRNAs with CRISPR-Local

Find 178 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATATTACTTTAGAATTAATT+TGG 0.220352 4_4:-61886402 Msa0693070:three_prime_UTR
TAGATCAAGCTAACCTTTCT+TGG 0.224166 4_4:-61886586 Msa0693070:three_prime_UTR
GATGGAATTGAACCGCTTTC+AGG 0.230161 4_4:-61887129 Msa0693070:CDS
TTCAAAGTTCAAATTCTTAG+AGG 0.303256 4_4:-61886724 Msa0693070:CDS
AGTTGGGCCACGACTTATAA+TGG 0.308961 4_4:-61886868 Msa0693070:CDS
ACGGGTACTAAGATGTGTTT+TGG 0.320299 4_4:+61887080 None:intergenic
TTCCGACTATTCTCAAATAT+CGG 0.320685 4_4:-61886897 Msa0693070:CDS
TTTCTTGGCTTGACCTTTGT+GGG 0.323305 4_4:-61886571 Msa0693070:three_prime_UTR
AAGTAATATGTTTCTCTGAT+TGG 0.351267 4_4:+61886416 None:intergenic
GCATCCCTCTCACCGGTTCC+AGG 0.356774 4_4:+61886670 None:intergenic
CTTTCTTGGCTTGACCTTTG+TGG 0.363705 4_4:-61886572 Msa0693070:three_prime_UTR
AGAGCATAGACTTTGGTGTT+AGG 0.364464 4_4:-61886506 Msa0693070:three_prime_UTR
GACCTTTGTGGGATTGTTCA+AGG 0.364760 4_4:-61886560 Msa0693070:three_prime_UTR
GAGAATAGTCGGAACCGATT+TGG 0.387243 4_4:+61886906 None:intergenic
ACACATCTTAGTACCCGTTA+TGG 0.396282 4_4:-61887075 Msa0693070:CDS
GAAGATAATTATTTGAAGCT+TGG 0.405476 4_4:-61886796 Msa0693070:CDS
TCCGACTATTCTCAAATATC+GGG 0.408506 4_4:-61886896 Msa0693070:CDS
GCCAACGCTATCAAACAGTT+TGG 0.414501 4_4:+61886834 None:intergenic
AACTGTTTGATAGCGTTGGC+TGG 0.415821 4_4:-61886831 Msa0693070:CDS
GAAAGGACCACATGAAAGTA+TGG 0.437117 4_4:+61887102 None:intergenic
TCGGTGATGGTGGTTGGTGG+TGG 0.442258 4_4:+61887488 None:intergenic
ATACAGAGGGAGAGTGATGA+TGG 0.448288 4_4:+61887525 None:intergenic
CTTTACGAATTTCCTGGAAC+CGG 0.450094 4_4:-61886682 Msa0693070:CDS
TGAAATTACCAATCCATAAC+GGG 0.462093 4_4:+61887062 None:intergenic
CAAATATCGGGGTAAGAGTT+GGG 0.464634 4_4:-61886884 Msa0693070:CDS
GATTACTAATCTATGACAAA+TGG 0.467072 4_4:+61886646 None:intergenic
TCAAATATCGGGGTAAGAGT+TGG 0.468409 4_4:-61886885 Msa0693070:CDS
TGAAGAACTTCCTTCCAAAT+CGG 0.469161 4_4:-61886920 Msa0693070:CDS
GTTGGGCCACGACTTATAAT+GGG 0.482324 4_4:-61886867 Msa0693070:CDS
TAAAGGTCGCCGGAAGGATG+TGG 0.497755 4_4:+61887449 None:intergenic
TTGTCGGTGATGGTGGTTGG+TGG 0.500922 4_4:+61887485 None:intergenic
ATGTCGTCTTCAAAATCAGC+TGG 0.502794 4_4:-61887314 Msa0693070:five_prime_UTR
TCTTAGTACCCGTTATGGAT+TGG 0.505736 4_4:-61887070 Msa0693070:intron
ACCAAACTGTTTGATAGCGT+TGG 0.508522 4_4:-61886835 Msa0693070:CDS
AATAGTGAAAAGTTATGTAC+TGG 0.509208 4_4:-61886483 Msa0693070:three_prime_UTR
ATGAAATTACCAATCCATAA+CGG 0.511215 4_4:+61887061 None:intergenic
CACAAAGGTCAAGCCAAGAA+AGG 0.514465 4_4:+61886573 None:intergenic
CCCCGATATTTGAGAATAGT+CGG 0.515602 4_4:+61886895 None:intergenic
CGAACAGATCCACATCCTTC+CGG 0.519644 4_4:-61887458 Msa0693070:five_prime_UTR
CGGAAGGATGTGGATCTGTT+CGG 0.521075 4_4:+61887459 None:intergenic
ACCTTCGACAAATATGCAAA+CGG 0.530039 4_4:-61887151 Msa0693070:CDS
GTGTACATGAAAGTTAATCT+TGG 0.531460 4_4:+61887255 None:intergenic
TAATGTGATATGAAATACTA+AGG 0.533146 4_4:+61887278 None:intergenic
TGTTCGGAAGTTGTCGGTGA+TGG 0.534909 4_4:+61887475 None:intergenic
ACTGTTTGATAGCGTTGGCT+GGG 0.539366 4_4:-61886830 Msa0693070:CDS
GAAGGTGATGATAACGAGGC+TGG 0.539673 4_4:+61887168 None:intergenic
TTACTTTAGAATTAATTTGG+TGG 0.543443 4_4:-61886399 Msa0693070:three_prime_UTR
TCCGTTTGCATATTTGTCGA+AGG 0.549182 4_4:+61887150 None:intergenic
GTTCTGCCCATTATAAGTCG+TGG 0.557426 4_4:+61886861 None:intergenic
GGTTGGTGGTGGAAAAGAAA+AGG 0.558273 4_4:+61887499 None:intergenic
AAGTTGTCGGTGATGGTGGT+TGG 0.562351 4_4:+61887482 None:intergenic
AAAGAAAAGGAAAATACAGA+GGG 0.564283 4_4:+61887512 None:intergenic
TCTTCAAAATCAGCTGGAAG+AGG 0.564849 4_4:-61887308 Msa0693070:five_prime_UTR
AAATGGTGCATCCCTCTCAC+CGG 0.578086 4_4:+61886663 None:intergenic
AGGAGTGCCATACTTTCATG+TGG 0.578309 4_4:-61887109 Msa0693070:CDS
TCGACAAATATGCAAACGGA+TGG 0.583245 4_4:-61887147 Msa0693070:CDS
GAGTAGCAGTCAAGTTGCTG+TGG 0.583739 4_4:-61887196 Msa0693070:CDS
TCTTCACATATGCTGCTGCT+CGG 0.585483 4_4:+61886937 None:intergenic
ATTTCCTGGAACCGGTGAGA+GGG 0.587927 4_4:-61886674 Msa0693070:CDS
AGTAGCAGTCAAGTTGCTGT+GGG 0.603556 4_4:-61887195 Msa0693070:CDS
GTAGGTTAGAGCATAGACTT+TGG 0.607501 4_4:-61886513 Msa0693070:three_prime_UTR
TGGATCTGTTCGGAAGTTGT+CGG 0.611775 4_4:+61887469 None:intergenic
TTAACTTTCATGTACACGGT+CGG 0.616082 4_4:-61887249 Msa0693070:exon
TCGGAAGTTGTCGGTGATGG+TGG 0.619536 4_4:+61887478 None:intergenic
TTTAAGTAAAGGTCGCCGGA+AGG 0.626587 4_4:+61887443 None:intergenic
AAAAGAAAAGGAAAATACAG+AGG 0.630802 4_4:+61887511 None:intergenic
AATTTCCTGGAACCGGTGAG+AGG 0.633829 4_4:-61886675 Msa0693070:CDS
ATAGTCGGAACCGATTTGGA+AGG 0.643344 4_4:+61886910 None:intergenic
AAGATTAACTTTCATGTACA+CGG 0.643809 4_4:-61887253 Msa0693070:exon
AAGTATGGCACTCCTGAAAG+CGG 0.678549 4_4:+61887117 None:intergenic
CCGACTATTCTCAAATATCG+GGG 0.681942 4_4:-61886895 Msa0693070:CDS
AACCTTGAACAATCCCACAA+AGG 0.707964 4_4:+61886558 None:intergenic
TGTCGAAGGTGATGATAACG+AGG 0.728967 4_4:+61887164 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATGTAATTTTTTTTAAGTAA+AGG + chr4_4:61886496-61886515 None:intergenic 10.0%
!!! ATATTACTTTAGAATTAATT+TGG - chr4_4:61887523-61887542 Msa0693070:five_prime_UTR 10.0%
!!! ATGTAATTTTTTTTAAGTAA+AGG + chr4_4:61886496-61886515 None:intergenic 10.0%
!!! ATATTACTTTAGAATTAATT+TGG - chr4_4:61887523-61887542 Msa0693070:five_prime_UTR 10.0%
!!! TATCAAATTAGATTTTCAAA+AGG - chr4_4:61886548-61886567 Msa0693070:three_prime_UTR 15.0%
!!! TATCAAATTAGATTTTCAAA+AGG - chr4_4:61886548-61886567 Msa0693070:three_prime_UTR 15.0%
!! TAATGTGATATGAAATACTA+AGG + chr4_4:61886650-61886669 None:intergenic 20.0%
!! TTATAGATCAAACTAAATGA+AGG - chr4_4:61886904-61886923 Msa0693070:CDS 20.0%
!! TAAATCTTGTATTGTATTGT+TGG - chr4_4:61886955-61886974 Msa0693070:intron 20.0%
!!! TTACTTTAGAATTAATTTGG+TGG - chr4_4:61887526-61887545 Msa0693070:five_prime_UTR 20.0%
!! TAATGTGATATGAAATACTA+AGG + chr4_4:61886650-61886669 None:intergenic 20.0%
!! TTATAGATCAAACTAAATGA+AGG - chr4_4:61886904-61886923 Msa0693070:CDS 20.0%
!! TAAATCTTGTATTGTATTGT+TGG - chr4_4:61886955-61886974 Msa0693070:intron 20.0%
!!! TTACTTTAGAATTAATTTGG+TGG - chr4_4:61887526-61887545 Msa0693070:five_prime_UTR 20.0%
! AAAGAAAAGGAAAATACAGA+GGG + chr4_4:61886416-61886435 None:intergenic 25.0%
! AAAAGAAAAGGAAAATACAG+AGG + chr4_4:61886417-61886436 None:intergenic 25.0%
! AAGATTAACTTTCATGTACA+CGG - chr4_4:61886672-61886691 Msa0693070:CDS 25.0%
! ATGAAATTACCAATCCATAA+CGG + chr4_4:61886867-61886886 None:intergenic 25.0%
!! ATCAAACTAAATGAAGGTTT+TGG - chr4_4:61886910-61886929 Msa0693070:CDS 25.0%
! TATTGTATTGTTGGCATATA+GGG - chr4_4:61886964-61886983 Msa0693070:intron 25.0%
!! GAAGATAATTATTTGAAGCT+TGG - chr4_4:61887129-61887148 Msa0693070:CDS 25.0%
! TTCAAAGTTCAAATTCTTAG+AGG - chr4_4:61887201-61887220 Msa0693070:CDS 25.0%
! GATTACTAATCTATGACAAA+TGG + chr4_4:61887282-61887301 None:intergenic 25.0%
!! GATCTAGTTTCAAAAAGAAA+AGG + chr4_4:61887325-61887344 None:intergenic 25.0%
! AATAGTGAAAAGTTATGTAC+TGG - chr4_4:61887442-61887461 Msa0693070:five_prime_UTR 25.0%
! AAGTAATATGTTTCTCTGAT+TGG + chr4_4:61887512-61887531 None:intergenic 25.0%
! AAAGAAAAGGAAAATACAGA+GGG + chr4_4:61886416-61886435 None:intergenic 25.0%
! AAAAGAAAAGGAAAATACAG+AGG + chr4_4:61886417-61886436 None:intergenic 25.0%
! AAGATTAACTTTCATGTACA+CGG - chr4_4:61886672-61886691 Msa0693070:CDS 25.0%
! ATGAAATTACCAATCCATAA+CGG + chr4_4:61886867-61886886 None:intergenic 25.0%
!! ATCAAACTAAATGAAGGTTT+TGG - chr4_4:61886910-61886929 Msa0693070:CDS 25.0%
! TATTGTATTGTTGGCATATA+GGG - chr4_4:61886964-61886983 Msa0693070:intron 25.0%
!! GAAGATAATTATTTGAAGCT+TGG - chr4_4:61887129-61887148 Msa0693070:CDS 25.0%
! TTCAAAGTTCAAATTCTTAG+AGG - chr4_4:61887201-61887220 Msa0693070:CDS 25.0%
! GATTACTAATCTATGACAAA+TGG + chr4_4:61887282-61887301 None:intergenic 25.0%
!! GATCTAGTTTCAAAAAGAAA+AGG + chr4_4:61887325-61887344 None:intergenic 25.0%
! AATAGTGAAAAGTTATGTAC+TGG - chr4_4:61887442-61887461 Msa0693070:five_prime_UTR 25.0%
! AAGTAATATGTTTCTCTGAT+TGG + chr4_4:61887512-61887531 None:intergenic 25.0%
!!! TTTTTTTAAGTAAAGGTCGC+CGG + chr4_4:61886489-61886508 None:intergenic 30.0%
GTGTACATGAAAGTTAATCT+TGG + chr4_4:61886673-61886692 None:intergenic 30.0%
!!! TACTAAGATGTGTTTTGGAA+AGG + chr4_4:61886843-61886862 None:intergenic 30.0%
TGAAATTACCAATCCATAAC+GGG + chr4_4:61886866-61886885 None:intergenic 30.0%
!!! ACTAAATGAAGGTTTTGGAA+AGG - chr4_4:61886915-61886934 Msa0693070:CDS 30.0%
GTATTGTATTGTTGGCATAT+AGG - chr4_4:61886963-61886982 Msa0693070:intron 30.0%
ATTGTATTGTTGGCATATAG+GGG - chr4_4:61886965-61886984 Msa0693070:intron 30.0%
TTCCGACTATTCTCAAATAT+CGG - chr4_4:61887028-61887047 Msa0693070:intron 30.0%
GGAAATTCGTAAAGCAAAAA+TGG + chr4_4:61887237-61887256 None:intergenic 30.0%
! TTTTTGCTTTACGAATTTCC+TGG - chr4_4:61887237-61887256 Msa0693070:CDS 30.0%
!!! GATACTTTTGTTTTGTGAGT+AGG - chr4_4:61887394-61887413 Msa0693070:five_prime_UTR 30.0%
!!! TTTTTTTAAGTAAAGGTCGC+CGG + chr4_4:61886489-61886508 None:intergenic 30.0%
GTGTACATGAAAGTTAATCT+TGG + chr4_4:61886673-61886692 None:intergenic 30.0%
!!! TACTAAGATGTGTTTTGGAA+AGG + chr4_4:61886843-61886862 None:intergenic 30.0%
TGAAATTACCAATCCATAAC+GGG + chr4_4:61886866-61886885 None:intergenic 30.0%
!!! ACTAAATGAAGGTTTTGGAA+AGG - chr4_4:61886915-61886934 Msa0693070:CDS 30.0%
GTATTGTATTGTTGGCATAT+AGG - chr4_4:61886963-61886982 Msa0693070:intron 30.0%
ATTGTATTGTTGGCATATAG+GGG - chr4_4:61886965-61886984 Msa0693070:intron 30.0%
TTCCGACTATTCTCAAATAT+CGG - chr4_4:61887028-61887047 Msa0693070:intron 30.0%
GGAAATTCGTAAAGCAAAAA+TGG + chr4_4:61887237-61887256 None:intergenic 30.0%
! TTTTTGCTTTACGAATTTCC+TGG - chr4_4:61887237-61887256 Msa0693070:CDS 30.0%
!!! GATACTTTTGTTTTGTGAGT+AGG - chr4_4:61887394-61887413 Msa0693070:five_prime_UTR 30.0%
TTAACTTTCATGTACACGGT+CGG - chr4_4:61886676-61886695 Msa0693070:CDS 35.0%
ACCTTCGACAAATATGCAAA+CGG - chr4_4:61886774-61886793 Msa0693070:CDS 35.0%
TGAAGAACTTCCTTCCAAAT+CGG - chr4_4:61887005-61887024 Msa0693070:intron 35.0%
TCCGACTATTCTCAAATATC+GGG - chr4_4:61887029-61887048 Msa0693070:intron 35.0%
TAGATCAAGCTAACCTTTCT+TGG - chr4_4:61887339-61887358 Msa0693070:five_prime_UTR 35.0%
TTAACTTTCATGTACACGGT+CGG - chr4_4:61886676-61886695 Msa0693070:CDS 35.0%
ACCTTCGACAAATATGCAAA+CGG - chr4_4:61886774-61886793 Msa0693070:CDS 35.0%
TGAAGAACTTCCTTCCAAAT+CGG - chr4_4:61887005-61887024 Msa0693070:intron 35.0%
TCCGACTATTCTCAAATATC+GGG - chr4_4:61887029-61887048 Msa0693070:intron 35.0%
TAGATCAAGCTAACCTTTCT+TGG - chr4_4:61887339-61887358 Msa0693070:five_prime_UTR 35.0%
ATGTCGTCTTCAAAATCAGC+TGG - chr4_4:61886611-61886630 Msa0693070:three_prime_UTR 40.0%
TCTTCAAAATCAGCTGGAAG+AGG - chr4_4:61886617-61886636 Msa0693070:three_prime_UTR 40.0%
TCCGTTTGCATATTTGTCGA+AGG + chr4_4:61886778-61886797 None:intergenic 40.0%
TCGACAAATATGCAAACGGA+TGG - chr4_4:61886778-61886797 Msa0693070:CDS 40.0%
GAAAGGACCACATGAAAGTA+TGG + chr4_4:61886826-61886845 None:intergenic 40.0%
! ACGGGTACTAAGATGTGTTT+TGG + chr4_4:61886848-61886867 None:intergenic 40.0%
ACACATCTTAGTACCCGTTA+TGG - chr4_4:61886850-61886869 Msa0693070:CDS 40.0%
TCTTAGTACCCGTTATGGAT+TGG - chr4_4:61886855-61886874 Msa0693070:CDS 40.0%
CCCCGATATTTGAGAATAGT+CGG + chr4_4:61887033-61887052 None:intergenic 40.0%
CCGACTATTCTCAAATATCG+GGG - chr4_4:61887030-61887049 Msa0693070:intron 40.0%
TCAAATATCGGGGTAAGAGT+TGG - chr4_4:61887040-61887059 Msa0693070:intron 40.0%
CAAATATCGGGGTAAGAGTT+GGG - chr4_4:61887041-61887060 Msa0693070:intron 40.0%
!! ACCAAACTGTTTGATAGCGT+TGG - chr4_4:61887090-61887109 Msa0693070:CDS 40.0%
GCTCATGAAAAACAGTGAAG+AGG - chr4_4:61887170-61887189 Msa0693070:CDS 40.0%
CTTTACGAATTTCCTGGAAC+CGG - chr4_4:61887243-61887262 Msa0693070:CDS 40.0%
! TTTCTTGGCTTGACCTTTGT+GGG - chr4_4:61887354-61887373 Msa0693070:five_prime_UTR 40.0%
AACCTTGAACAATCCCACAA+AGG + chr4_4:61887370-61887389 None:intergenic 40.0%
GTAGGTTAGAGCATAGACTT+TGG - chr4_4:61887412-61887431 Msa0693070:five_prime_UTR 40.0%
!! AGAGCATAGACTTTGGTGTT+AGG - chr4_4:61887419-61887438 Msa0693070:five_prime_UTR 40.0%
ATGTCGTCTTCAAAATCAGC+TGG - chr4_4:61886611-61886630 Msa0693070:three_prime_UTR 40.0%
TCTTCAAAATCAGCTGGAAG+AGG - chr4_4:61886617-61886636 Msa0693070:three_prime_UTR 40.0%
TCCGTTTGCATATTTGTCGA+AGG + chr4_4:61886778-61886797 None:intergenic 40.0%
TCGACAAATATGCAAACGGA+TGG - chr4_4:61886778-61886797 Msa0693070:CDS 40.0%
GAAAGGACCACATGAAAGTA+TGG + chr4_4:61886826-61886845 None:intergenic 40.0%
! ACGGGTACTAAGATGTGTTT+TGG + chr4_4:61886848-61886867 None:intergenic 40.0%
ACACATCTTAGTACCCGTTA+TGG - chr4_4:61886850-61886869 Msa0693070:CDS 40.0%
TCTTAGTACCCGTTATGGAT+TGG - chr4_4:61886855-61886874 Msa0693070:CDS 40.0%
CCCCGATATTTGAGAATAGT+CGG + chr4_4:61887033-61887052 None:intergenic 40.0%
CCGACTATTCTCAAATATCG+GGG - chr4_4:61887030-61887049 Msa0693070:intron 40.0%
TCAAATATCGGGGTAAGAGT+TGG - chr4_4:61887040-61887059 Msa0693070:intron 40.0%
CAAATATCGGGGTAAGAGTT+GGG - chr4_4:61887041-61887060 Msa0693070:intron 40.0%
!! ACCAAACTGTTTGATAGCGT+TGG - chr4_4:61887090-61887109 Msa0693070:CDS 40.0%
GCTCATGAAAAACAGTGAAG+AGG - chr4_4:61887170-61887189 Msa0693070:CDS 40.0%
CTTTACGAATTTCCTGGAAC+CGG - chr4_4:61887243-61887262 Msa0693070:CDS 40.0%
! TTTCTTGGCTTGACCTTTGT+GGG - chr4_4:61887354-61887373 Msa0693070:five_prime_UTR 40.0%
AACCTTGAACAATCCCACAA+AGG + chr4_4:61887370-61887389 None:intergenic 40.0%
GTAGGTTAGAGCATAGACTT+TGG - chr4_4:61887412-61887431 Msa0693070:five_prime_UTR 40.0%
!! AGAGCATAGACTTTGGTGTT+AGG - chr4_4:61887419-61887438 Msa0693070:five_prime_UTR 40.0%
ATACAGAGGGAGAGTGATGA+TGG + chr4_4:61886403-61886422 None:intergenic 45.0%
!! GGTTGGTGGTGGAAAAGAAA+AGG + chr4_4:61886429-61886448 None:intergenic 45.0%
TGGATCTGTTCGGAAGTTGT+CGG + chr4_4:61886459-61886478 None:intergenic 45.0%
TTTAAGTAAAGGTCGCCGGA+AGG + chr4_4:61886485-61886504 None:intergenic 45.0%
! AGTAGCAGTCAAGTTGCTGT+GGG - chr4_4:61886730-61886749 Msa0693070:CDS 45.0%
! TGTCGAAGGTGATGATAACG+AGG + chr4_4:61886764-61886783 None:intergenic 45.0%
GATGGAATTGAACCGCTTTC+AGG - chr4_4:61886796-61886815 Msa0693070:CDS 45.0%
AAGTATGGCACTCCTGAAAG+CGG + chr4_4:61886811-61886830 None:intergenic 45.0%
!! AGGAGTGCCATACTTTCATG+TGG - chr4_4:61886816-61886835 Msa0693070:CDS 45.0%
TCTTCACATATGCTGCTGCT+CGG + chr4_4:61886991-61887010 None:intergenic 45.0%
!! ATAGTCGGAACCGATTTGGA+AGG + chr4_4:61887018-61887037 None:intergenic 45.0%
GAGAATAGTCGGAACCGATT+TGG + chr4_4:61887022-61887041 None:intergenic 45.0%
AGTTGGGCCACGACTTATAA+TGG - chr4_4:61887057-61887076 Msa0693070:intron 45.0%
GTTGGGCCACGACTTATAAT+GGG - chr4_4:61887058-61887077 Msa0693070:intron 45.0%
GTTCTGCCCATTATAAGTCG+TGG + chr4_4:61887067-61887086 None:intergenic 45.0%
GCCAACGCTATCAAACAGTT+TGG + chr4_4:61887094-61887113 None:intergenic 45.0%
!! AACTGTTTGATAGCGTTGGC+TGG - chr4_4:61887094-61887113 Msa0693070:CDS 45.0%
!! ACTGTTTGATAGCGTTGGCT+GGG - chr4_4:61887095-61887114 Msa0693070:CDS 45.0%
CACAAAGGTCAAGCCAAGAA+AGG + chr4_4:61887355-61887374 None:intergenic 45.0%
! CTTTCTTGGCTTGACCTTTG+TGG - chr4_4:61887353-61887372 Msa0693070:five_prime_UTR 45.0%
GACCTTTGTGGGATTGTTCA+AGG - chr4_4:61887365-61887384 Msa0693070:five_prime_UTR 45.0%
ATACAGAGGGAGAGTGATGA+TGG + chr4_4:61886403-61886422 None:intergenic 45.0%
!! GGTTGGTGGTGGAAAAGAAA+AGG + chr4_4:61886429-61886448 None:intergenic 45.0%
TGGATCTGTTCGGAAGTTGT+CGG + chr4_4:61886459-61886478 None:intergenic 45.0%
TTTAAGTAAAGGTCGCCGGA+AGG + chr4_4:61886485-61886504 None:intergenic 45.0%
! AGTAGCAGTCAAGTTGCTGT+GGG - chr4_4:61886730-61886749 Msa0693070:CDS 45.0%
! TGTCGAAGGTGATGATAACG+AGG + chr4_4:61886764-61886783 None:intergenic 45.0%
GATGGAATTGAACCGCTTTC+AGG - chr4_4:61886796-61886815 Msa0693070:CDS 45.0%
AAGTATGGCACTCCTGAAAG+CGG + chr4_4:61886811-61886830 None:intergenic 45.0%
!! AGGAGTGCCATACTTTCATG+TGG - chr4_4:61886816-61886835 Msa0693070:CDS 45.0%
TCTTCACATATGCTGCTGCT+CGG + chr4_4:61886991-61887010 None:intergenic 45.0%
!! ATAGTCGGAACCGATTTGGA+AGG + chr4_4:61887018-61887037 None:intergenic 45.0%
GAGAATAGTCGGAACCGATT+TGG + chr4_4:61887022-61887041 None:intergenic 45.0%
AGTTGGGCCACGACTTATAA+TGG - chr4_4:61887057-61887076 Msa0693070:intron 45.0%
GTTGGGCCACGACTTATAAT+GGG - chr4_4:61887058-61887077 Msa0693070:intron 45.0%
GTTCTGCCCATTATAAGTCG+TGG + chr4_4:61887067-61887086 None:intergenic 45.0%
GCCAACGCTATCAAACAGTT+TGG + chr4_4:61887094-61887113 None:intergenic 45.0%
!! AACTGTTTGATAGCGTTGGC+TGG - chr4_4:61887094-61887113 Msa0693070:CDS 45.0%
!! ACTGTTTGATAGCGTTGGCT+GGG - chr4_4:61887095-61887114 Msa0693070:CDS 45.0%
CACAAAGGTCAAGCCAAGAA+AGG + chr4_4:61887355-61887374 None:intergenic 45.0%
! CTTTCTTGGCTTGACCTTTG+TGG - chr4_4:61887353-61887372 Msa0693070:five_prime_UTR 45.0%
GACCTTTGTGGGATTGTTCA+AGG - chr4_4:61887365-61887384 Msa0693070:five_prime_UTR 45.0%
!! AAGTTGTCGGTGATGGTGGT+TGG + chr4_4:61886446-61886465 None:intergenic 50.0%
TGTTCGGAAGTTGTCGGTGA+TGG + chr4_4:61886453-61886472 None:intergenic 50.0%
CGGAAGGATGTGGATCTGTT+CGG + chr4_4:61886469-61886488 None:intergenic 50.0%
CGAACAGATCCACATCCTTC+CGG - chr4_4:61886467-61886486 Msa0693070:three_prime_UTR 50.0%
! GAGTAGCAGTCAAGTTGCTG+TGG - chr4_4:61886729-61886748 Msa0693070:CDS 50.0%
! GAAGGTGATGATAACGAGGC+TGG + chr4_4:61886760-61886779 None:intergenic 50.0%
AATTTCCTGGAACCGGTGAG+AGG - chr4_4:61887250-61887269 Msa0693070:exon 50.0%
ATTTCCTGGAACCGGTGAGA+GGG - chr4_4:61887251-61887270 Msa0693070:exon 50.0%
AAATGGTGCATCCCTCTCAC+CGG + chr4_4:61887265-61887284 None:intergenic 50.0%
!! AAGTTGTCGGTGATGGTGGT+TGG + chr4_4:61886446-61886465 None:intergenic 50.0%
TGTTCGGAAGTTGTCGGTGA+TGG + chr4_4:61886453-61886472 None:intergenic 50.0%
CGGAAGGATGTGGATCTGTT+CGG + chr4_4:61886469-61886488 None:intergenic 50.0%
CGAACAGATCCACATCCTTC+CGG - chr4_4:61886467-61886486 Msa0693070:three_prime_UTR 50.0%
! GAGTAGCAGTCAAGTTGCTG+TGG - chr4_4:61886729-61886748 Msa0693070:CDS 50.0%
! GAAGGTGATGATAACGAGGC+TGG + chr4_4:61886760-61886779 None:intergenic 50.0%
AATTTCCTGGAACCGGTGAG+AGG - chr4_4:61887250-61887269 Msa0693070:exon 50.0%
ATTTCCTGGAACCGGTGAGA+GGG - chr4_4:61887251-61887270 Msa0693070:exon 50.0%
AAATGGTGCATCCCTCTCAC+CGG + chr4_4:61887265-61887284 None:intergenic 50.0%
!! TTGTCGGTGATGGTGGTTGG+TGG + chr4_4:61886443-61886462 None:intergenic 55.0%
! TCGGAAGTTGTCGGTGATGG+TGG + chr4_4:61886450-61886469 None:intergenic 55.0%
TAAAGGTCGCCGGAAGGATG+TGG + chr4_4:61886479-61886498 None:intergenic 55.0%
!! TTGTCGGTGATGGTGGTTGG+TGG + chr4_4:61886443-61886462 None:intergenic 55.0%
! TCGGAAGTTGTCGGTGATGG+TGG + chr4_4:61886450-61886469 None:intergenic 55.0%
TAAAGGTCGCCGGAAGGATG+TGG + chr4_4:61886479-61886498 None:intergenic 55.0%
!! TCGGTGATGGTGGTTGGTGG+TGG + chr4_4:61886440-61886459 None:intergenic 60.0%
!! TCGGTGATGGTGGTTGGTGG+TGG + chr4_4:61886440-61886459 None:intergenic 60.0%
!! GCATCCCTCTCACCGGTTCC+AGG + chr4_4:61887258-61887277 None:intergenic 65.0%
!! GCATCCCTCTCACCGGTTCC+AGG + chr4_4:61887258-61887277 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 61886388 61887559 61886388 ID=Msa0693070;Name=Msa0693070
chr4_4 mRNA 61886388 61887559 61886388 ID=Msa0693070-mRNA-1;Parent=Msa0693070;Name=Msa0693070-mRNA-1;_AED=0.05;_eAED=0.05;_QI=297|1|1|1|1|1|2|263|167
chr4_4 exon 61886388 61886962 61886388 ID=Msa0693070-mRNA-1:exon:17202;Parent=Msa0693070-mRNA-1
chr4_4 exon 61887071 61887559 61887071 ID=Msa0693070-mRNA-1:exon:17201;Parent=Msa0693070-mRNA-1
chr4_4 five_prime_UTR 61887263 61887559 61887263 ID=Msa0693070-mRNA-1:five_prime_utr;Parent=Msa0693070-mRNA-1
chr4_4 CDS 61887071 61887262 61887071 ID=Msa0693070-mRNA-1:cds;Parent=Msa0693070-mRNA-1
chr4_4 CDS 61886651 61886962 61886651 ID=Msa0693070-mRNA-1:cds;Parent=Msa0693070-mRNA-1
chr4_4 three_prime_UTR 61886388 61886650 61886388 ID=Msa0693070-mRNA-1:three_prime_utr;Parent=Msa0693070-mRNA-1
Gene Sequence

>Msa0693070

ATGTACACGGTCGGTCTATCACTAATTTCACATTTATTTTCAGTGAGTAGCAGTCAAGTTGCTGTGGGAAATCCAGCCTCGTTATCATCACCTTCGACAAATATGCAAACGGATGGAATTGAACCGCTTTCAGGAGTGCCATACTTTCATGTGGTCCTTTCCAAAACACATCTTAGTACCCGTTATGGATTGGGGCCGAGCAGCAGCATATGTGAAGAACTTCCTTCCAAATCGGTTCCGACTATTCTCAAATATCGGGGTAAGAGTTGGGCCACGACTTATAATGGGCAGAACAAAACCAAACTGTTTGATAGCGTTGGCTGGGAAAAATTTGCTGAAGATAATTATTTGAAGCTTGGAGACGCTTGTGTTTTTGAGCTCATGAAAAACAGTGAAGAGGAAATCGTCTTCAAAGTTCAAATTCTTAGAGGTGAAGAAGAGCCATTTTTGCTTTACGAATTTCCTGGAACCGGTGAGAGGGATGCACCATTTGTCATAGATTAG
Protein sequence

>Msa0693070

MYTVGLSLISHLFSVSSSQVAVGNPASLSSPSTNMQTDGIEPLSGVPYFHVVLSKTHLSTRYGLGPSSSICEELPSKSVPTILKYRGKSWATTYNGQNKTKLFDSVGWEKFAEDNYLKLGDACVFELMKNSEEEIVFKVQILRGEEEPFLLYEFPGTGERDAPFVID*