AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0610320

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0610320 MtrunA17_Chr4g0043351 86.452 155 18 1 16 167 8 162 4.30e-95 272
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 74 sgRNAs with CRISPR-Local

Find 180 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATATTACTTTAGAATTAATT+TGG 0.220352 4_2:-58027728 Msa0610320:three_prime_UTR
TAGATCAAGCTAACCTTTCT+TGG 0.224166 4_2:-58027912 Msa0610320:three_prime_UTR
GATGGAATTGAACCGCTTTC+AGG 0.230161 4_2:-58028455 Msa0610320:CDS
TTCAAAGTTCAAATTCTTAG+AGG 0.303256 4_2:-58028050 Msa0610320:CDS
AGTTGGGCCACGACTTATAA+TGG 0.308961 4_2:-58028194 Msa0610320:CDS
ACGGGGACTAAGATGTGTTT+TGG 0.312922 4_2:+58028406 None:intergenic
TTCCGACTATTCTCAAATAT+CGG 0.320685 4_2:-58028223 Msa0610320:CDS
TTTCTTGGCTTGACCTTTGT+GGG 0.323305 4_2:-58027897 Msa0610320:three_prime_UTR
AAGTAATATGTTTCTCTGAT+TGG 0.351267 4_2:+58027742 None:intergenic
GCATCCCTCTCACCGGTTCC+AGG 0.356774 4_2:+58027996 None:intergenic
CTTTCTTGGCTTGACCTTTG+TGG 0.363705 4_2:-58027898 Msa0610320:three_prime_UTR
AGAGCATAGACTTTGGTGTT+AGG 0.364464 4_2:-58027832 Msa0610320:three_prime_UTR
GACCTTTGTGGGATTGTTCA+AGG 0.364760 4_2:-58027886 Msa0610320:three_prime_UTR
TGAAATTACCAATCCATAAC+GGG 0.384987 4_2:+58028388 None:intergenic
GAGAATAGTCGGAACCGATT+TGG 0.387243 4_2:+58028232 None:intergenic
ACACATCTTAGTCCCCGTTA+TGG 0.404304 4_2:-58028401 Msa0610320:CDS
GAAGATAATTATTTGAAGCT+TGG 0.405476 4_2:-58028122 Msa0610320:CDS
TCCGACTATTCTCAAATATC+GGG 0.408506 4_2:-58028222 Msa0610320:CDS
GCCAACGCTATCAAACAGTT+TGG 0.414501 4_2:+58028160 None:intergenic
AACTGTTTGATAGCGTTGGC+TGG 0.415821 4_2:-58028157 Msa0610320:CDS
GAAAGGACCACATGAAAGTA+TGG 0.437117 4_2:+58028428 None:intergenic
TCGGTGATGGTGGTTGGTGG+TGG 0.442258 4_2:+58028814 None:intergenic
CTTTACGAATTTCCTGGAAC+CGG 0.450094 4_2:-58028008 Msa0610320:CDS
CAAATATCGGGGTAAGAGTT+GGG 0.464634 4_2:-58028210 Msa0610320:CDS
GATTACTAATCTATGACAAA+TGG 0.467072 4_2:+58027972 None:intergenic
TCAAATATCGGGGTAAGAGT+TGG 0.468409 4_2:-58028211 Msa0610320:CDS
TGAAGAACTTCCTTCCAAAT+CGG 0.469161 4_2:-58028246 Msa0610320:CDS
GTTGGGCCACGACTTATAAT+GGG 0.482324 4_2:-58028193 Msa0610320:CDS
TAAAGGTCGCCGGAAGGATG+TGG 0.497755 4_2:+58028775 None:intergenic
ATGAAATTACCAATCCATAA+CGG 0.499697 4_2:+58028387 None:intergenic
TTGTCGGTGATGGTGGTTGG+TGG 0.500922 4_2:+58028811 None:intergenic
ATGTCGTCTTCAAAATCAGC+TGG 0.502794 4_2:-58028640 Msa0610320:five_prime_UTR
ACCAAACTGTTTGATAGCGT+TGG 0.508522 4_2:-58028161 Msa0610320:CDS
AATAGTGAAAAGTTATGTAC+TGG 0.509208 4_2:-58027809 Msa0610320:three_prime_UTR
CACAAAGGTCAAGCCAAGAA+AGG 0.514465 4_2:+58027899 None:intergenic
CCCCGATATTTGAGAATAGT+CGG 0.515602 4_2:+58028221 None:intergenic
TCTTAGTCCCCGTTATGGAT+TGG 0.515821 4_2:-58028396 Msa0610320:intron
CGAACAGATCCACATCCTTC+CGG 0.519644 4_2:-58028784 Msa0610320:five_prime_UTR
CGGAAGGATGTGGATCTGTT+CGG 0.521075 4_2:+58028785 None:intergenic
ACCTTCGACAAATATGCAAA+CGG 0.530039 4_2:-58028477 Msa0610320:CDS
GTGTACATGAAAGTTAATCT+TGG 0.531460 4_2:+58028581 None:intergenic
TAATGTGATATGAAATACTA+AGG 0.533146 4_2:+58028604 None:intergenic
TGTTCGGAAGTTGTCGGTGA+TGG 0.534909 4_2:+58028801 None:intergenic
ACTGTTTGATAGCGTTGGCT+GGG 0.539366 4_2:-58028156 Msa0610320:CDS
GAAGGTGATGATAACGAGGC+TGG 0.539673 4_2:+58028494 None:intergenic
TTACTTTAGAATTAATTTGG+TGG 0.543443 4_2:-58027725 Msa0610320:three_prime_UTR
TCCGTTTGCATATTTGTCGA+AGG 0.549182 4_2:+58028476 None:intergenic
GTTCTGCCCATTATAAGTCG+TGG 0.557426 4_2:+58028187 None:intergenic
GGTTGGTGGTGGAAAAGAAA+AGG 0.558273 4_2:+58028825 None:intergenic
AAGTTGTCGGTGATGGTGGT+TGG 0.562351 4_2:+58028808 None:intergenic
AAAGAAAAGGAAAATACAGA+GGG 0.564283 4_2:+58028838 None:intergenic
TCTTCAAAATCAGCTGGAAG+AGG 0.564849 4_2:-58028634 Msa0610320:five_prime_UTR
CAGAGGGAGAGTGATGATGA+TGG 0.572504 4_2:+58028854 None:intergenic
AAATGGTGCATCCCTCTCAC+CGG 0.578086 4_2:+58027989 None:intergenic
AGGAGTGCCATACTTTCATG+TGG 0.578309 4_2:-58028435 Msa0610320:CDS
TCGACAAATATGCAAACGGA+TGG 0.583245 4_2:-58028473 Msa0610320:CDS
GAGTAGCAGTCAAGTTGCTG+TGG 0.583739 4_2:-58028522 Msa0610320:CDS
TCTTCACATATGCTGCTGCT+CGG 0.585483 4_2:+58028263 None:intergenic
ATTTCCTGGAACCGGTGAGA+GGG 0.587927 4_2:-58028000 Msa0610320:CDS
AGTAGCAGTCAAGTTGCTGT+GGG 0.603556 4_2:-58028521 Msa0610320:CDS
GTAGGTTAGAGCATAGACTT+TGG 0.607501 4_2:-58027839 Msa0610320:three_prime_UTR
TGGATCTGTTCGGAAGTTGT+CGG 0.611775 4_2:+58028795 None:intergenic
TTAACTTTCATGTACACGGT+CGG 0.616082 4_2:-58028575 Msa0610320:exon
TCGGAAGTTGTCGGTGATGG+TGG 0.619536 4_2:+58028804 None:intergenic
TTTAAGTAAAGGTCGCCGGA+AGG 0.626587 4_2:+58028769 None:intergenic
AAAAGAAAAGGAAAATACAG+AGG 0.630802 4_2:+58028837 None:intergenic
AATTTCCTGGAACCGGTGAG+AGG 0.633829 4_2:-58028001 Msa0610320:CDS
ATAGTCGGAACCGATTTGGA+AGG 0.643344 4_2:+58028236 None:intergenic
AAGATTAACTTTCATGTACA+CGG 0.643809 4_2:-58028579 Msa0610320:exon
AAGTATGGCACTCCTGAAAG+CGG 0.678549 4_2:+58028443 None:intergenic
CCGACTATTCTCAAATATCG+GGG 0.681942 4_2:-58028221 Msa0610320:CDS
AACCTTGAACAATCCCACAA+AGG 0.707964 4_2:+58027884 None:intergenic
GAAATTACCAATCCATAACG+GGG 0.716232 4_2:+58028389 None:intergenic
TGTCGAAGGTGATGATAACG+AGG 0.728967 4_2:+58028490 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATGTAATTTTTTTTAAGTAA+AGG + chr4_2:58027825-58027844 None:intergenic 10.0%
!!! ATATTACTTTAGAATTAATT+TGG - chr4_2:58028852-58028871 Msa0610320:five_prime_UTR 10.0%
!!! ATGTAATTTTTTTTAAGTAA+AGG + chr4_2:58027825-58027844 None:intergenic 10.0%
!!! ATATTACTTTAGAATTAATT+TGG - chr4_2:58028852-58028871 Msa0610320:five_prime_UTR 10.0%
!!! TATCAAATTAGATTTTCAAA+AGG - chr4_2:58027877-58027896 Msa0610320:three_prime_UTR 15.0%
!!! TATCAAATTAGATTTTCAAA+AGG - chr4_2:58027877-58027896 Msa0610320:three_prime_UTR 15.0%
!! TAATGTGATATGAAATACTA+AGG + chr4_2:58027979-58027998 None:intergenic 20.0%
!! TTATAGATCAAACTAAATGA+AGG - chr4_2:58028233-58028252 Msa0610320:CDS 20.0%
!! TAAATCTTGTATTGTATTGT+TGG - chr4_2:58028284-58028303 Msa0610320:intron 20.0%
!!! TTACTTTAGAATTAATTTGG+TGG - chr4_2:58028855-58028874 Msa0610320:five_prime_UTR 20.0%
!! TAATGTGATATGAAATACTA+AGG + chr4_2:58027979-58027998 None:intergenic 20.0%
!! TTATAGATCAAACTAAATGA+AGG - chr4_2:58028233-58028252 Msa0610320:CDS 20.0%
!! TAAATCTTGTATTGTATTGT+TGG - chr4_2:58028284-58028303 Msa0610320:intron 20.0%
!!! TTACTTTAGAATTAATTTGG+TGG - chr4_2:58028855-58028874 Msa0610320:five_prime_UTR 20.0%
! AAAGAAAAGGAAAATACAGA+GGG + chr4_2:58027745-58027764 None:intergenic 25.0%
! AAAAGAAAAGGAAAATACAG+AGG + chr4_2:58027746-58027765 None:intergenic 25.0%
! AAGATTAACTTTCATGTACA+CGG - chr4_2:58028001-58028020 Msa0610320:CDS 25.0%
! ATGAAATTACCAATCCATAA+CGG + chr4_2:58028196-58028215 None:intergenic 25.0%
!! ATCAAACTAAATGAAGGTTT+TGG - chr4_2:58028239-58028258 Msa0610320:CDS 25.0%
! TATTGTATTGTTGGCATATA+GGG - chr4_2:58028293-58028312 Msa0610320:intron 25.0%
!! GAAGATAATTATTTGAAGCT+TGG - chr4_2:58028458-58028477 Msa0610320:CDS 25.0%
! TTCAAAGTTCAAATTCTTAG+AGG - chr4_2:58028530-58028549 Msa0610320:CDS 25.0%
! GATTACTAATCTATGACAAA+TGG + chr4_2:58028611-58028630 None:intergenic 25.0%
!! GATCTAGTTTCAAAAAGAAA+AGG + chr4_2:58028654-58028673 None:intergenic 25.0%
! AATAGTGAAAAGTTATGTAC+TGG - chr4_2:58028771-58028790 Msa0610320:five_prime_UTR 25.0%
! AAGTAATATGTTTCTCTGAT+TGG + chr4_2:58028841-58028860 None:intergenic 25.0%
! AAAGAAAAGGAAAATACAGA+GGG + chr4_2:58027745-58027764 None:intergenic 25.0%
! AAAAGAAAAGGAAAATACAG+AGG + chr4_2:58027746-58027765 None:intergenic 25.0%
! AAGATTAACTTTCATGTACA+CGG - chr4_2:58028001-58028020 Msa0610320:CDS 25.0%
! ATGAAATTACCAATCCATAA+CGG + chr4_2:58028196-58028215 None:intergenic 25.0%
!! ATCAAACTAAATGAAGGTTT+TGG - chr4_2:58028239-58028258 Msa0610320:CDS 25.0%
! TATTGTATTGTTGGCATATA+GGG - chr4_2:58028293-58028312 Msa0610320:intron 25.0%
!! GAAGATAATTATTTGAAGCT+TGG - chr4_2:58028458-58028477 Msa0610320:CDS 25.0%
! TTCAAAGTTCAAATTCTTAG+AGG - chr4_2:58028530-58028549 Msa0610320:CDS 25.0%
! GATTACTAATCTATGACAAA+TGG + chr4_2:58028611-58028630 None:intergenic 25.0%
!! GATCTAGTTTCAAAAAGAAA+AGG + chr4_2:58028654-58028673 None:intergenic 25.0%
! AATAGTGAAAAGTTATGTAC+TGG - chr4_2:58028771-58028790 Msa0610320:five_prime_UTR 25.0%
! AAGTAATATGTTTCTCTGAT+TGG + chr4_2:58028841-58028860 None:intergenic 25.0%
!!! TTTTTTTAAGTAAAGGTCGC+CGG + chr4_2:58027818-58027837 None:intergenic 30.0%
GTGTACATGAAAGTTAATCT+TGG + chr4_2:58028002-58028021 None:intergenic 30.0%
TGAAATTACCAATCCATAAC+GGG + chr4_2:58028195-58028214 None:intergenic 30.0%
!!! ACTAAATGAAGGTTTTGGAA+AGG - chr4_2:58028244-58028263 Msa0610320:CDS 30.0%
GTATTGTATTGTTGGCATAT+AGG - chr4_2:58028292-58028311 Msa0610320:intron 30.0%
ATTGTATTGTTGGCATATAG+GGG - chr4_2:58028294-58028313 Msa0610320:intron 30.0%
TTCCGACTATTCTCAAATAT+CGG - chr4_2:58028357-58028376 Msa0610320:intron 30.0%
GGAAATTCGTAAAGCAAAAA+TGG + chr4_2:58028566-58028585 None:intergenic 30.0%
! TTTTTGCTTTACGAATTTCC+TGG - chr4_2:58028566-58028585 Msa0610320:CDS 30.0%
!!! GATACTTTTGTTTTGTGAGT+AGG - chr4_2:58028723-58028742 Msa0610320:five_prime_UTR 30.0%
!!! TTTTTTTAAGTAAAGGTCGC+CGG + chr4_2:58027818-58027837 None:intergenic 30.0%
GTGTACATGAAAGTTAATCT+TGG + chr4_2:58028002-58028021 None:intergenic 30.0%
TGAAATTACCAATCCATAAC+GGG + chr4_2:58028195-58028214 None:intergenic 30.0%
!!! ACTAAATGAAGGTTTTGGAA+AGG - chr4_2:58028244-58028263 Msa0610320:CDS 30.0%
GTATTGTATTGTTGGCATAT+AGG - chr4_2:58028292-58028311 Msa0610320:intron 30.0%
ATTGTATTGTTGGCATATAG+GGG - chr4_2:58028294-58028313 Msa0610320:intron 30.0%
TTCCGACTATTCTCAAATAT+CGG - chr4_2:58028357-58028376 Msa0610320:intron 30.0%
GGAAATTCGTAAAGCAAAAA+TGG + chr4_2:58028566-58028585 None:intergenic 30.0%
! TTTTTGCTTTACGAATTTCC+TGG - chr4_2:58028566-58028585 Msa0610320:CDS 30.0%
!!! GATACTTTTGTTTTGTGAGT+AGG - chr4_2:58028723-58028742 Msa0610320:five_prime_UTR 30.0%
TTAACTTTCATGTACACGGT+CGG - chr4_2:58028005-58028024 Msa0610320:CDS 35.0%
ACCTTCGACAAATATGCAAA+CGG - chr4_2:58028103-58028122 Msa0610320:CDS 35.0%
!!! GACTAAGATGTGTTTTGGAA+AGG + chr4_2:58028172-58028191 None:intergenic 35.0%
GAAATTACCAATCCATAACG+GGG + chr4_2:58028194-58028213 None:intergenic 35.0%
TGAAGAACTTCCTTCCAAAT+CGG - chr4_2:58028334-58028353 Msa0610320:intron 35.0%
TCCGACTATTCTCAAATATC+GGG - chr4_2:58028358-58028377 Msa0610320:intron 35.0%
TAGATCAAGCTAACCTTTCT+TGG - chr4_2:58028668-58028687 Msa0610320:five_prime_UTR 35.0%
TTAACTTTCATGTACACGGT+CGG - chr4_2:58028005-58028024 Msa0610320:CDS 35.0%
ACCTTCGACAAATATGCAAA+CGG - chr4_2:58028103-58028122 Msa0610320:CDS 35.0%
!!! GACTAAGATGTGTTTTGGAA+AGG + chr4_2:58028172-58028191 None:intergenic 35.0%
GAAATTACCAATCCATAACG+GGG + chr4_2:58028194-58028213 None:intergenic 35.0%
TGAAGAACTTCCTTCCAAAT+CGG - chr4_2:58028334-58028353 Msa0610320:intron 35.0%
TCCGACTATTCTCAAATATC+GGG - chr4_2:58028358-58028377 Msa0610320:intron 35.0%
TAGATCAAGCTAACCTTTCT+TGG - chr4_2:58028668-58028687 Msa0610320:five_prime_UTR 35.0%
ATGTCGTCTTCAAAATCAGC+TGG - chr4_2:58027940-58027959 Msa0610320:three_prime_UTR 40.0%
TCTTCAAAATCAGCTGGAAG+AGG - chr4_2:58027946-58027965 Msa0610320:three_prime_UTR 40.0%
TCCGTTTGCATATTTGTCGA+AGG + chr4_2:58028107-58028126 None:intergenic 40.0%
TCGACAAATATGCAAACGGA+TGG - chr4_2:58028107-58028126 Msa0610320:CDS 40.0%
GAAAGGACCACATGAAAGTA+TGG + chr4_2:58028155-58028174 None:intergenic 40.0%
CCCCGATATTTGAGAATAGT+CGG + chr4_2:58028362-58028381 None:intergenic 40.0%
CCGACTATTCTCAAATATCG+GGG - chr4_2:58028359-58028378 Msa0610320:intron 40.0%
TCAAATATCGGGGTAAGAGT+TGG - chr4_2:58028369-58028388 Msa0610320:intron 40.0%
CAAATATCGGGGTAAGAGTT+GGG - chr4_2:58028370-58028389 Msa0610320:intron 40.0%
!! ACCAAACTGTTTGATAGCGT+TGG - chr4_2:58028419-58028438 Msa0610320:CDS 40.0%
GCTCATGAAAAACAGTGAAG+AGG - chr4_2:58028499-58028518 Msa0610320:CDS 40.0%
CTTTACGAATTTCCTGGAAC+CGG - chr4_2:58028572-58028591 Msa0610320:exon 40.0%
! TTTCTTGGCTTGACCTTTGT+GGG - chr4_2:58028683-58028702 Msa0610320:five_prime_UTR 40.0%
AACCTTGAACAATCCCACAA+AGG + chr4_2:58028699-58028718 None:intergenic 40.0%
GTAGGTTAGAGCATAGACTT+TGG - chr4_2:58028741-58028760 Msa0610320:five_prime_UTR 40.0%
!! AGAGCATAGACTTTGGTGTT+AGG - chr4_2:58028748-58028767 Msa0610320:five_prime_UTR 40.0%
ATGTCGTCTTCAAAATCAGC+TGG - chr4_2:58027940-58027959 Msa0610320:three_prime_UTR 40.0%
TCTTCAAAATCAGCTGGAAG+AGG - chr4_2:58027946-58027965 Msa0610320:three_prime_UTR 40.0%
TCCGTTTGCATATTTGTCGA+AGG + chr4_2:58028107-58028126 None:intergenic 40.0%
TCGACAAATATGCAAACGGA+TGG - chr4_2:58028107-58028126 Msa0610320:CDS 40.0%
GAAAGGACCACATGAAAGTA+TGG + chr4_2:58028155-58028174 None:intergenic 40.0%
CCCCGATATTTGAGAATAGT+CGG + chr4_2:58028362-58028381 None:intergenic 40.0%
CCGACTATTCTCAAATATCG+GGG - chr4_2:58028359-58028378 Msa0610320:intron 40.0%
TCAAATATCGGGGTAAGAGT+TGG - chr4_2:58028369-58028388 Msa0610320:intron 40.0%
CAAATATCGGGGTAAGAGTT+GGG - chr4_2:58028370-58028389 Msa0610320:intron 40.0%
!! ACCAAACTGTTTGATAGCGT+TGG - chr4_2:58028419-58028438 Msa0610320:CDS 40.0%
GCTCATGAAAAACAGTGAAG+AGG - chr4_2:58028499-58028518 Msa0610320:CDS 40.0%
CTTTACGAATTTCCTGGAAC+CGG - chr4_2:58028572-58028591 Msa0610320:exon 40.0%
! TTTCTTGGCTTGACCTTTGT+GGG - chr4_2:58028683-58028702 Msa0610320:five_prime_UTR 40.0%
AACCTTGAACAATCCCACAA+AGG + chr4_2:58028699-58028718 None:intergenic 40.0%
GTAGGTTAGAGCATAGACTT+TGG - chr4_2:58028741-58028760 Msa0610320:five_prime_UTR 40.0%
!! AGAGCATAGACTTTGGTGTT+AGG - chr4_2:58028748-58028767 Msa0610320:five_prime_UTR 40.0%
!! GGTTGGTGGTGGAAAAGAAA+AGG + chr4_2:58027758-58027777 None:intergenic 45.0%
TGGATCTGTTCGGAAGTTGT+CGG + chr4_2:58027788-58027807 None:intergenic 45.0%
TTTAAGTAAAGGTCGCCGGA+AGG + chr4_2:58027814-58027833 None:intergenic 45.0%
! AGTAGCAGTCAAGTTGCTGT+GGG - chr4_2:58028059-58028078 Msa0610320:CDS 45.0%
! TGTCGAAGGTGATGATAACG+AGG + chr4_2:58028093-58028112 None:intergenic 45.0%
GATGGAATTGAACCGCTTTC+AGG - chr4_2:58028125-58028144 Msa0610320:CDS 45.0%
AAGTATGGCACTCCTGAAAG+CGG + chr4_2:58028140-58028159 None:intergenic 45.0%
!! AGGAGTGCCATACTTTCATG+TGG - chr4_2:58028145-58028164 Msa0610320:CDS 45.0%
! ACGGGGACTAAGATGTGTTT+TGG + chr4_2:58028177-58028196 None:intergenic 45.0%
ACACATCTTAGTCCCCGTTA+TGG - chr4_2:58028179-58028198 Msa0610320:CDS 45.0%
TCTTAGTCCCCGTTATGGAT+TGG - chr4_2:58028184-58028203 Msa0610320:CDS 45.0%
TCTTCACATATGCTGCTGCT+CGG + chr4_2:58028320-58028339 None:intergenic 45.0%
!! ATAGTCGGAACCGATTTGGA+AGG + chr4_2:58028347-58028366 None:intergenic 45.0%
GAGAATAGTCGGAACCGATT+TGG + chr4_2:58028351-58028370 None:intergenic 45.0%
AGTTGGGCCACGACTTATAA+TGG - chr4_2:58028386-58028405 Msa0610320:intron 45.0%
GTTGGGCCACGACTTATAAT+GGG - chr4_2:58028387-58028406 Msa0610320:intron 45.0%
GTTCTGCCCATTATAAGTCG+TGG + chr4_2:58028396-58028415 None:intergenic 45.0%
GCCAACGCTATCAAACAGTT+TGG + chr4_2:58028423-58028442 None:intergenic 45.0%
!! AACTGTTTGATAGCGTTGGC+TGG - chr4_2:58028423-58028442 Msa0610320:CDS 45.0%
!! ACTGTTTGATAGCGTTGGCT+GGG - chr4_2:58028424-58028443 Msa0610320:CDS 45.0%
CACAAAGGTCAAGCCAAGAA+AGG + chr4_2:58028684-58028703 None:intergenic 45.0%
! CTTTCTTGGCTTGACCTTTG+TGG - chr4_2:58028682-58028701 Msa0610320:five_prime_UTR 45.0%
GACCTTTGTGGGATTGTTCA+AGG - chr4_2:58028694-58028713 Msa0610320:five_prime_UTR 45.0%
!! GGTTGGTGGTGGAAAAGAAA+AGG + chr4_2:58027758-58027777 None:intergenic 45.0%
TGGATCTGTTCGGAAGTTGT+CGG + chr4_2:58027788-58027807 None:intergenic 45.0%
TTTAAGTAAAGGTCGCCGGA+AGG + chr4_2:58027814-58027833 None:intergenic 45.0%
! AGTAGCAGTCAAGTTGCTGT+GGG - chr4_2:58028059-58028078 Msa0610320:CDS 45.0%
! TGTCGAAGGTGATGATAACG+AGG + chr4_2:58028093-58028112 None:intergenic 45.0%
GATGGAATTGAACCGCTTTC+AGG - chr4_2:58028125-58028144 Msa0610320:CDS 45.0%
AAGTATGGCACTCCTGAAAG+CGG + chr4_2:58028140-58028159 None:intergenic 45.0%
!! AGGAGTGCCATACTTTCATG+TGG - chr4_2:58028145-58028164 Msa0610320:CDS 45.0%
! ACGGGGACTAAGATGTGTTT+TGG + chr4_2:58028177-58028196 None:intergenic 45.0%
ACACATCTTAGTCCCCGTTA+TGG - chr4_2:58028179-58028198 Msa0610320:CDS 45.0%
TCTTAGTCCCCGTTATGGAT+TGG - chr4_2:58028184-58028203 Msa0610320:CDS 45.0%
TCTTCACATATGCTGCTGCT+CGG + chr4_2:58028320-58028339 None:intergenic 45.0%
!! ATAGTCGGAACCGATTTGGA+AGG + chr4_2:58028347-58028366 None:intergenic 45.0%
GAGAATAGTCGGAACCGATT+TGG + chr4_2:58028351-58028370 None:intergenic 45.0%
AGTTGGGCCACGACTTATAA+TGG - chr4_2:58028386-58028405 Msa0610320:intron 45.0%
GTTGGGCCACGACTTATAAT+GGG - chr4_2:58028387-58028406 Msa0610320:intron 45.0%
GTTCTGCCCATTATAAGTCG+TGG + chr4_2:58028396-58028415 None:intergenic 45.0%
GCCAACGCTATCAAACAGTT+TGG + chr4_2:58028423-58028442 None:intergenic 45.0%
!! AACTGTTTGATAGCGTTGGC+TGG - chr4_2:58028423-58028442 Msa0610320:CDS 45.0%
!! ACTGTTTGATAGCGTTGGCT+GGG - chr4_2:58028424-58028443 Msa0610320:CDS 45.0%
CACAAAGGTCAAGCCAAGAA+AGG + chr4_2:58028684-58028703 None:intergenic 45.0%
! CTTTCTTGGCTTGACCTTTG+TGG - chr4_2:58028682-58028701 Msa0610320:five_prime_UTR 45.0%
GACCTTTGTGGGATTGTTCA+AGG - chr4_2:58028694-58028713 Msa0610320:five_prime_UTR 45.0%
! CAGAGGGAGAGTGATGATGA+TGG + chr4_2:58027729-58027748 None:intergenic 50.0%
!! AAGTTGTCGGTGATGGTGGT+TGG + chr4_2:58027775-58027794 None:intergenic 50.0%
TGTTCGGAAGTTGTCGGTGA+TGG + chr4_2:58027782-58027801 None:intergenic 50.0%
CGGAAGGATGTGGATCTGTT+CGG + chr4_2:58027798-58027817 None:intergenic 50.0%
CGAACAGATCCACATCCTTC+CGG - chr4_2:58027796-58027815 Msa0610320:three_prime_UTR 50.0%
! GAGTAGCAGTCAAGTTGCTG+TGG - chr4_2:58028058-58028077 Msa0610320:CDS 50.0%
! GAAGGTGATGATAACGAGGC+TGG + chr4_2:58028089-58028108 None:intergenic 50.0%
AATTTCCTGGAACCGGTGAG+AGG - chr4_2:58028579-58028598 Msa0610320:exon 50.0%
ATTTCCTGGAACCGGTGAGA+GGG - chr4_2:58028580-58028599 Msa0610320:exon 50.0%
AAATGGTGCATCCCTCTCAC+CGG + chr4_2:58028594-58028613 None:intergenic 50.0%
! CAGAGGGAGAGTGATGATGA+TGG + chr4_2:58027729-58027748 None:intergenic 50.0%
!! AAGTTGTCGGTGATGGTGGT+TGG + chr4_2:58027775-58027794 None:intergenic 50.0%
TGTTCGGAAGTTGTCGGTGA+TGG + chr4_2:58027782-58027801 None:intergenic 50.0%
CGGAAGGATGTGGATCTGTT+CGG + chr4_2:58027798-58027817 None:intergenic 50.0%
CGAACAGATCCACATCCTTC+CGG - chr4_2:58027796-58027815 Msa0610320:three_prime_UTR 50.0%
! GAGTAGCAGTCAAGTTGCTG+TGG - chr4_2:58028058-58028077 Msa0610320:CDS 50.0%
! GAAGGTGATGATAACGAGGC+TGG + chr4_2:58028089-58028108 None:intergenic 50.0%
AATTTCCTGGAACCGGTGAG+AGG - chr4_2:58028579-58028598 Msa0610320:exon 50.0%
ATTTCCTGGAACCGGTGAGA+GGG - chr4_2:58028580-58028599 Msa0610320:exon 50.0%
AAATGGTGCATCCCTCTCAC+CGG + chr4_2:58028594-58028613 None:intergenic 50.0%
!! TTGTCGGTGATGGTGGTTGG+TGG + chr4_2:58027772-58027791 None:intergenic 55.0%
! TCGGAAGTTGTCGGTGATGG+TGG + chr4_2:58027779-58027798 None:intergenic 55.0%
TAAAGGTCGCCGGAAGGATG+TGG + chr4_2:58027808-58027827 None:intergenic 55.0%
!! TTGTCGGTGATGGTGGTTGG+TGG + chr4_2:58027772-58027791 None:intergenic 55.0%
! TCGGAAGTTGTCGGTGATGG+TGG + chr4_2:58027779-58027798 None:intergenic 55.0%
TAAAGGTCGCCGGAAGGATG+TGG + chr4_2:58027808-58027827 None:intergenic 55.0%
!! TCGGTGATGGTGGTTGGTGG+TGG + chr4_2:58027769-58027788 None:intergenic 60.0%
!! TCGGTGATGGTGGTTGGTGG+TGG + chr4_2:58027769-58027788 None:intergenic 60.0%
!! GCATCCCTCTCACCGGTTCC+AGG + chr4_2:58028587-58028606 None:intergenic 65.0%
!! GCATCCCTCTCACCGGTTCC+AGG + chr4_2:58028587-58028606 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 58027714 58028888 58027714 ID=Msa0610320;Name=Msa0610320
chr4_2 mRNA 58027714 58028888 58027714 ID=Msa0610320-mRNA-1;Parent=Msa0610320;Name=Msa0610320-mRNA-1;_AED=0.05;_eAED=0.05;_QI=300|1|1|1|1|1|2|263|167
chr4_2 exon 58027714 58028288 58027714 ID=Msa0610320-mRNA-1:exon:15208;Parent=Msa0610320-mRNA-1
chr4_2 exon 58028397 58028888 58028397 ID=Msa0610320-mRNA-1:exon:15207;Parent=Msa0610320-mRNA-1
chr4_2 five_prime_UTR 58028589 58028888 58028589 ID=Msa0610320-mRNA-1:five_prime_utr;Parent=Msa0610320-mRNA-1
chr4_2 CDS 58028397 58028588 58028397 ID=Msa0610320-mRNA-1:cds;Parent=Msa0610320-mRNA-1
chr4_2 CDS 58027977 58028288 58027977 ID=Msa0610320-mRNA-1:cds;Parent=Msa0610320-mRNA-1
chr4_2 three_prime_UTR 58027714 58027976 58027714 ID=Msa0610320-mRNA-1:three_prime_utr;Parent=Msa0610320-mRNA-1
Gene Sequence

>Msa0610320

ATGTACACGGTCGGTCTATCACTAATTTCACATTTATTTTCAGTGAGTAGCAGTCAAGTTGCTGTGGGAAATCCAGCCTCGTTATCATCACCTTCGACAAATATGCAAACGGATGGAATTGAACCGCTTTCAGGAGTGCCATACTTTCATGTGGTCCTTTCCAAAACACATCTTAGTCCCCGTTATGGATTGGGGCCGAGCAGCAGCATATGTGAAGAACTTCCTTCCAAATCGGTTCCGACTATTCTCAAATATCGGGGTAAGAGTTGGGCCACGACTTATAATGGGCAGAACAAAACCAAACTGTTTGATAGCGTTGGCTGGGAAAAATTTGCTGAAGATAATTATTTGAAGCTTGGAGACGCTTGTGTTTTTGAGCTCATGAAAAACAGTGAAGAGGAAATCGTCTTCAAAGTTCAAATTCTTAGAGGTGAAGAAGAGCCATTTTTGCTTTACGAATTTCCTGGAACCGGTGAGAGGGATGCACCATTTGTCATAGATTAG
Protein sequence

>Msa0610320

MYTVGLSLISHLFSVSSSQVAVGNPASLSSPSTNMQTDGIEPLSGVPYFHVVLSKTHLSPRYGLGPSSSICEELPSKSVPTILKYRGKSWATTYNGQNKTKLFDSVGWEKFAEDNYLKLGDACVFELMKNSEEEIVFKVQILRGEEEPFLLYEFPGTGERDAPFVID*