AlfalfaGEDB | Msa0657520

M. sativa cultivar zhongmu-4 / Msa0657520

KEGG: -
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005856 GO:0005938 GO:0006355 GO:0007275 GO:0008150 GO:0009553 GO:0009889 GO:0009890 GO:0009892 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0019219 GO:0019222 GO:0030863 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0045892 GO:0045934 GO:0048229 GO:0048519 GO:0048523 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0060255 GO:0065007 GO:0070013 GO:0071944 GO:0080090 GO:0099568 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
PFAM: PF04844.16

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0657520	XP_003629846.1	80.654	367	22	7	1	367	1	318	6.68e-172	494

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0657520	sp\|Q8VZN1\|OFP5_ARATH	52.143	140	61	3	233	370	210	345	2.20e-36	139

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0657520	G7L799	80.654	367	22	7	1	367	1	318	3.19e-172	494

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TFs/TRs:

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Gene ID	Type	Classification
Msa0657520	TF	OFP

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0618620	Msa0657520	0.837492	5.033858e-57	-8.615850e-47
Msa0657520	Msa0701600	0.950829	7.316665e-109	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0657520	MtrunA17_Chr8g0379681	80.654	367	22	7	1	367	1	318	6.15e-176	494
Msa0657520	MtrunA17_Chr2g0284471	47.257	237	95	5	150	367	82	307	4.15e-53	180
Msa0657520	MtrunA17_Chr8g0379691	92.308	52	4	0	366	417	14	65	1.23e-26	101
Msa0657520	MtrunA17_Chr5g0432531	43.284	134	57	5	234	366	130	245	1.08e-22	96.7
Msa0657520	MtrunA17_Chr7g0244571	63.333	60	22	0	307	366	281	340	4.79e-20	90.9
Msa0657520	MtrunA17_Chr4g0013081	62.712	59	22	0	308	366	315	373	8.38e-19	87.4
Msa0657520	MtrunA17_Chr8g0390531	56.667	60	26	0	307	366	258	317	2.83e-15	76.3
Msa0657520	MtrunA17_Chr1g0177881	50.769	65	32	0	305	369	100	164	2.43e-13	68.2
Msa0657520	MtrunA17_Chr4g0025901	43.939	66	37	0	306	371	98	163	1.54e-12	65.9
Msa0657520	MtrunA17_Chr5g0435131	42.254	71	41	0	306	376	90	160	4.71e-12	63.9

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0657520	AT4G18830.1	52.143	140	61	3	233	370	210	345	2.23e-37	139
Msa0657520	AT5G58360.1	47.573	103	50	1	269	367	182	284	1.08e-21	95.1
Msa0657520	AT1G06920.1	56.716	67	29	0	301	367	237	303	2.21e-20	91.7
Msa0657520	AT2G30400.1	56.716	67	29	0	300	366	245	311	7.87e-19	87.0
Msa0657520	AT5G01840.1	60.714	56	22	0	311	366	202	257	7.25e-16	77.8
Msa0657520	AT5G19650.1	55.172	58	26	0	307	364	154	211	7.40e-16	76.6
Msa0657520	AT3G52525.1	50.794	63	31	0	302	364	61	123	5.85e-12	63.9
Msa0657520	AT2G18500.1	49.153	59	30	0	306	364	225	283	8.52e-12	66.2

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Find 69 sgRNAs with CRISPR-Local

Find 183 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGATGAAGAAAGAAGAAG+AGG	0.484787	4_3:-67200950	Msa0657520:CDS
AAATGGTGTGAATCAATGTG+AGG	0.711307	4_3:-67201142	Msa0657520:CDS
AACAACTCTTCCATTTCTTC+TGG	0.277466	4_3:+67200805	None:intergenic
AACCTGCCTCTATTCCCACA+AGG	0.669707	4_3:+67201576	None:intergenic
AAGAGTACATTCTCCAAAGA+TGG	0.518216	4_3:-67201031	Msa0657520:CDS
AAGCAAAGGTTAACAAAAGG+AGG	0.522101	4_3:-67201113	Msa0657520:CDS
AATCAAACAATGAAGTGGAG+TGG	0.599426	4_3:-67201825	None:intergenic
AATGATATCATGGTATTCAT+TGG	0.398859	4_3:+67200759	None:intergenic
AATTGTGATGAACCATCAGA+CGG	0.617022	4_3:+67201600	None:intergenic
ACATCCAAACTGTACATTAC+TGG	0.306963	4_3:+67201459	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CTACATAAGAGGGAAATAAT+TGG	+	chr4_3:67199362-67199381	None:intergenic	30.0%
AAAGATGAAGAAAGAAGAAG+AGG	-	chr4_3:67199614-67199633	Msa0657520:intron	30.0%
CGACTATTTACAATTCTAAC+AGG	+	chr4_3:67199924-67199943	None:intergenic	30.0%
AGTTCTTTGTGTATGCAAAT+CGG	-	chr4_3:67200017-67200036	Msa0657520:intron	30.0%
TCCAAAAGTAGAAACAGTTT+AGG	-	chr4_3:67200180-67200199	Msa0657520:intron	30.0%
GCATATTACTACAATTACAG+TGG	-	chr4_3:67200216-67200235	Msa0657520:intron	30.0%
AAGACATCTATTATGGTGAT+AGG	+	chr4_3:67200618-67200637	None:intergenic	30.0%
TATCATATCAGGCATCTATA+AGG	-	chr4_3:67200845-67200864	Msa0657520:CDS	30.0%
CTGGAAATATTATCTAGTCA+TGG	+	chr4_3:67201247-67201266	None:intergenic	30.0%
GAATATAAAAGTCCTCAAAG+TGG	+	chr4_3:67201495-67201514	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_3	gene	67198748	67201838	67198748	ID=Msa0657520;Name=Msa0657520
chr4_3	mRNA	67198748	67201838	67198748	ID=Msa0657520-mRNA-1;Parent=Msa0657520;Name=Msa0657520-mRNA-1;_AED=0.10;_eAED=0.12;_QI=0\|0\|0\|1\|1\|1\|2\|0\|417
chr4_3	exon	67200741	67201838	67200741	ID=Msa0657520-mRNA-1:exon:19351;Parent=Msa0657520-mRNA-1
chr4_3	exon	67198748	67198903	67198748	ID=Msa0657520-mRNA-1:exon:19350;Parent=Msa0657520-mRNA-1
chr4_3	CDS	67200741	67201838	67200741	ID=Msa0657520-mRNA-1:cds;Parent=Msa0657520-mRNA-1
chr4_3	CDS	67198748	67198903	67198748	ID=Msa0657520-mRNA-1:cds;Parent=Msa0657520-mRNA-1

Gene Sequence

>Msa0657520

ATGAAGTGGAGTGGAAGAAAACCTTCTTCAGCTTCAGCTTCTTCTTCTTCTTCTTCTAAGCCTTCCTTCATCTCTCATGTATCCTCTTTTTCATGGCTGTCAAAGTTCAAGCAAATGAAAATCAACTCAGACTCAGAAGCAAAATCCGAAAAGCTGATGCAGAACTCCCCGTCTGATGCAACGACAACAATCAAGCATTATCCCACTCAGAACTCCCCGTCTGATGGTTCATCACAATTTCCTTGTGGGAATAGAGGCAGGTTTTATGGAGGTGGTGAGGATGATGCATTCTGGAGATTGTCATTTGGTGAAGAGTTTACTAATGACCAAAAGAAGAAAAGTAAAGATATTCTGAAGCCAGTAATGTACAGTTTGGATGTGAAAAGACATGGAAGAAGAAGAAGAAGAAGAGAAGATAGAAAATTGCTTAATGAAACAAAGAGTGCAAAGGAAGTTGAGTGCTTAAAGAGGAGATATGAGAGGAAAGCACAAAGAGCTTTACAAGAGCAGCTCTTGAAATTACAGAATGCAGCAGAGGAAGCTGAATTTGTTGCATCAAGTAAATGTAAATCCTTAGAAAAGGATGAGCTCCAATTTGAATCACCAAGAACCCAATTATTTCCCTCTTATGTAGATGCAGAAAGTTCTGGTCTTGGATTTCAAAGTGTCGGAGAAAATGGTGTGAATCAATGTGAGGAGTTTAAAGCAAAGGTTAACAAAAGGAGGGAATCAGTTCATGTGAGCAGAGAATTACAGAGGAGGAAACCAAAGCAGAGTTCCAAGGTAAGAGTACATTCTCCAAAGATGGCTACTAAAGTCGAAATACGCAAAATGAAGGCGATAGAAGACAAAAAGAAAGCAAAACTAAAGATGAAGAAAGAAGAAGAGGAAATGGTGGAGGAAACAGAAGAAGGGTTAGATAGTTTTGCAGTTGTGAAGTGTTCTTTGGATCCACAACAAGATTTTAGAGATTCAATGATTGAGATGATCAAAGAGAAAGAGATAAGCCAGCCAGAAGAAATGGAAGAGTTGTTAGCATGCTATTTGAGTTTGAATTCCAATGAATACCATGATATCATTATCAAAGCATTTCGACAGACAACAAATGAAGCTCGTTTAAAAGAGATTAGTATGCTGATTAATATGGACAAAACAAGTAAAGCCAATACTACTATATTGCTGAATAGAGATTCAGTTCATCCTAGGAATGATTCCCAAAATCAGTTCCAAAAACAGGAATATGAAGTAGCATAA

Protein sequence

>Msa0657520

MKWSGRKPSSASASSSSSSKPSFISHVSSFSWLSKFKQMKINSDSEAKSEKLMQNSPSDATTTIKHYPTQNSPSDGSSQFPCGNRGRFYGGGEDDAFWRLSFGEEFTNDQKKKSKDILKPVMYSLDVKRHGRRRRRREDRKLLNETKSAKEVECLKRRYERKAQRALQEQLLKLQNAAEEAEFVASSKCKSLEKDELQFESPRTQLFPSYVDAESSGLGFQSVGENGVNQCEEFKAKVNKRRESVHVSRELQRRKPKQSSKVRVHSPKMATKVEIRKMKAIEDKKKAKLKMKKEEEEMVEETEEGLDSFAVVKCSLDPQQDFRDSMIEMIKEKEISQPEEMEELLACYLSLNSNEYHDIIIKAFRQTTNEARLKEISMLINMDKTSKANTTILLNRDSVHPRNDSQNQFQKQEYEVA*