Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0667170 | XP_003604575.1 | 96.911 | 259 | 7 | 1 | 1 | 259 | 17 | 274 | 0.0 | 517 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0667170 | G7JLT9 | 96.911 | 259 | 7 | 1 | 1 | 259 | 17 | 274 | 0.0 | 517 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0626890 | Msa0667170 | 0.802804 | 4.715019e-49 | -8.615850e-47 |
| Msa0665040 | Msa0667170 | 0.800423 | 1.451370e-48 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0667170 | MtrunA17_Chr4g0005671 | 96.911 | 259 | 7 | 1 | 1 | 259 | 17 | 274 | 0.0 | 517 |
| Msa0667170 | MtrunA17_Chr7g0243141 | 46.324 | 272 | 89 | 13 | 1 | 249 | 23 | 260 | 2.01e-51 | 169 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 35 sgRNAs with CRISPR-Local
Find 90 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTCTCGAAACTTTCTTCCCT+TGG | 0.318412 | 4_4:-7397360 | Msa0667170:CDS |
| TCTTTGTCATCAAGTTCATT+AGG | 0.362522 | 4_4:-7397913 | Msa0667170:CDS |
| GATTCTGATAAATAGCATTC+TGG | 0.384127 | 4_4:+7397448 | None:intergenic |
| CCATTCCTACCAGTATCTTT+CGG | 0.386168 | 4_4:+7397832 | None:intergenic |
| TCTTCCGGACGCAACCGCTT+TGG | 0.410420 | 4_4:-7397539 | Msa0667170:CDS |
| GAATGCTATTTATCAGAATC+AGG | 0.425031 | 4_4:-7397445 | Msa0667170:CDS |
| GTTTCGAGACCACATCCCTT+TGG | 0.425269 | 4_4:+7397373 | None:intergenic |
| TCGTGGAAACAAACGATGGC+AGG | 0.437134 | 4_4:+7397795 | None:intergenic |
| CCAGGAAAACCGAAAGATAC+TGG | 0.444180 | 4_4:-7397841 | Msa0667170:CDS |
| GCAGCCAAAGCGGTTGCGTC+CGG | 0.447146 | 4_4:+7397535 | None:intergenic |
| TCTTCTTCTCCAAAGGGATG+TGG | 0.458927 | 4_4:-7397382 | Msa0667170:CDS |
| GGTTCTTTCTCCCATGAAAA+CGG | 0.468783 | 4_4:+7397862 | None:intergenic |
| GTCGTGGAGCATGGCAGTAC+AGG | 0.469526 | 4_4:+7397301 | None:intergenic |
| CAGCGCAACTCGAGAGAGTC+GGG | 0.479961 | 4_4:-7397256 | Msa0667170:CDS |
| CTTTCTTCACGTTCGTGCAA+GGG | 0.499824 | 4_4:+7397203 | None:intergenic |
| TGATCATGTAAGTAGGAGAT+TGG | 0.506183 | 4_4:+7397569 | None:intergenic |
| TCTTTCTTCACGTTCGTGCA+AGG | 0.523409 | 4_4:+7397202 | None:intergenic |
| CAAAGATGTGTCTGCAACGA+CGG | 0.528117 | 4_4:+7397930 | None:intergenic |
| CTGCAGATTTGTCGTAGTCG+TGG | 0.532103 | 4_4:+7397285 | None:intergenic |
| CCGAAAGATACTGGTAGGAA+TGG | 0.534229 | 4_4:-7397832 | Msa0667170:CDS |
| TGATCTTTACTCAGATGCTA+TGG | 0.539239 | 4_4:-7397704 | Msa0667170:CDS |
| ATTATGTCAACACGTCGTCT+TGG | 0.552256 | 4_4:-7397955 | Msa0667170:CDS |
| TTATGCTTCATAGTTCTCCA+AGG | 0.554836 | 4_4:+7397343 | None:intergenic |
| AAACGGTTGATCATGTAAGT+AGG | 0.561511 | 4_4:+7397562 | None:intergenic |
| TTTGTTTCCACGAAAAGAAG+CGG | 0.584227 | 4_4:-7397785 | Msa0667170:CDS |
| CGTGGAAACAAACGATGGCA+GGG | 0.601100 | 4_4:+7397796 | None:intergenic |
| TATGCTTCATAGTTCTCCAA+GGG | 0.609801 | 4_4:+7397344 | None:intergenic |
| TTAAAGCTTGCAGAGTGTAG+TGG | 0.611037 | 4_4:-7397595 | Msa0667170:CDS |
| TCAGCGCAACTCGAGAGAGT+CGG | 0.612829 | 4_4:-7397257 | Msa0667170:CDS |
| GAAAACCGAAAGATACTGGT+AGG | 0.618943 | 4_4:-7397837 | Msa0667170:CDS |
| TTTGTCGTAGTCGTGGAGCA+TGG | 0.626564 | 4_4:+7397292 | None:intergenic |
| CGGTTGCGTCCGGAAGAAAA+CGG | 0.640456 | 4_4:+7397545 | None:intergenic |
| AGACGATGATGCAGCCAAAG+CGG | 0.673928 | 4_4:+7397525 | None:intergenic |
| AGTCGGGACAGAAGAAACAA+CGG | 0.729959 | 4_4:-7397240 | Msa0667170:CDS |
| GGAAACAAACGATGGCAGGG+TGG | 0.758125 | 4_4:+7397799 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GATGTAAAAAATGATTGTGA+TGG | - | chr4_4:7397396-7397415 | Msa0667170:CDS | 25.0% |
| ! | GATGTAAAAAATGATTGTGA+TGG | - | chr4_4:7397396-7397415 | Msa0667170:CDS | 25.0% |
| TCTTTGTCATCAAGTTCATT+AGG | - | chr4_4:7397234-7397253 | Msa0667170:CDS | 30.0% | |
| ! | AAAAAATGATTGTGATGGAG+AGG | - | chr4_4:7397401-7397420 | Msa0667170:CDS | 30.0% |
| GATTCTGATAAATAGCATTC+TGG | + | chr4_4:7397702-7397721 | None:intergenic | 30.0% | |
| ! | GAATGCTATTTATCAGAATC+AGG | - | chr4_4:7397702-7397721 | Msa0667170:CDS | 30.0% |
| TCTTTGTCATCAAGTTCATT+AGG | - | chr4_4:7397234-7397253 | Msa0667170:CDS | 30.0% | |
| ! | AAAAAATGATTGTGATGGAG+AGG | - | chr4_4:7397401-7397420 | Msa0667170:CDS | 30.0% |
| GATTCTGATAAATAGCATTC+TGG | + | chr4_4:7397702-7397721 | None:intergenic | 30.0% | |
| ! | GAATGCTATTTATCAGAATC+AGG | - | chr4_4:7397702-7397721 | Msa0667170:CDS | 30.0% |
| ! | TGAAAGAGTTCCGTTTTCAT+GGG | - | chr4_4:7397275-7397294 | Msa0667170:CDS | 35.0% |
| TTTGTTTCCACGAAAAGAAG+CGG | - | chr4_4:7397362-7397381 | Msa0667170:CDS | 35.0% | |
| TGATCTTTACTCAGATGCTA+TGG | - | chr4_4:7397443-7397462 | Msa0667170:CDS | 35.0% | |
| TGATCATGTAAGTAGGAGAT+TGG | + | chr4_4:7397581-7397600 | None:intergenic | 35.0% | |
| AAACGGTTGATCATGTAAGT+AGG | + | chr4_4:7397588-7397607 | None:intergenic | 35.0% | |
| TTCTTCTTCTTCTTCTCCAA+AGG | - | chr4_4:7397758-7397777 | Msa0667170:CDS | 35.0% | |
| TCTTCTTCTTCTTCTCCAAA+GGG | - | chr4_4:7397759-7397778 | Msa0667170:CDS | 35.0% | |
| TATGCTTCATAGTTCTCCAA+GGG | + | chr4_4:7397806-7397825 | None:intergenic | 35.0% | |
| TTATGCTTCATAGTTCTCCA+AGG | + | chr4_4:7397807-7397826 | None:intergenic | 35.0% | |
| ! | TGAAAGAGTTCCGTTTTCAT+GGG | - | chr4_4:7397275-7397294 | Msa0667170:CDS | 35.0% |
| TTTGTTTCCACGAAAAGAAG+CGG | - | chr4_4:7397362-7397381 | Msa0667170:CDS | 35.0% | |
| TGATCTTTACTCAGATGCTA+TGG | - | chr4_4:7397443-7397462 | Msa0667170:CDS | 35.0% | |
| TGATCATGTAAGTAGGAGAT+TGG | + | chr4_4:7397581-7397600 | None:intergenic | 35.0% | |
| AAACGGTTGATCATGTAAGT+AGG | + | chr4_4:7397588-7397607 | None:intergenic | 35.0% | |
| TTCTTCTTCTTCTTCTCCAA+AGG | - | chr4_4:7397758-7397777 | Msa0667170:CDS | 35.0% | |
| TCTTCTTCTTCTTCTCCAAA+GGG | - | chr4_4:7397759-7397778 | Msa0667170:CDS | 35.0% | |
| TATGCTTCATAGTTCTCCAA+GGG | + | chr4_4:7397806-7397825 | None:intergenic | 35.0% | |
| TTATGCTTCATAGTTCTCCA+AGG | + | chr4_4:7397807-7397826 | None:intergenic | 35.0% | |
| ! | GTGAAAGAGTTCCGTTTTCA+TGG | - | chr4_4:7397274-7397293 | Msa0667170:CDS | 40.0% |
| GGTTCTTTCTCCCATGAAAA+CGG | + | chr4_4:7397288-7397307 | None:intergenic | 40.0% | |
| ! | TTTTCATGGGAGAAAGAACC+AGG | - | chr4_4:7397288-7397307 | Msa0667170:CDS | 40.0% |
| GAAAACCGAAAGATACTGGT+AGG | - | chr4_4:7397310-7397329 | Msa0667170:CDS | 40.0% | |
| CCATTCCTACCAGTATCTTT+CGG | + | chr4_4:7397318-7397337 | None:intergenic | 40.0% | |
| ! | CTTTTCGTGGAAACAAACGA+TGG | + | chr4_4:7397359-7397378 | None:intergenic | 40.0% |
| TTAAAGCTTGCAGAGTGTAG+TGG | - | chr4_4:7397552-7397571 | Msa0667170:CDS | 40.0% | |
| ! | CATGATCAACCGTTTTCTTC+CGG | - | chr4_4:7397593-7397612 | Msa0667170:CDS | 40.0% |
| ! | GTGAAAGAGTTCCGTTTTCA+TGG | - | chr4_4:7397274-7397293 | Msa0667170:CDS | 40.0% |
| GGTTCTTTCTCCCATGAAAA+CGG | + | chr4_4:7397288-7397307 | None:intergenic | 40.0% | |
| ! | TTTTCATGGGAGAAAGAACC+AGG | - | chr4_4:7397288-7397307 | Msa0667170:CDS | 40.0% |
| GAAAACCGAAAGATACTGGT+AGG | - | chr4_4:7397310-7397329 | Msa0667170:CDS | 40.0% | |
| CCATTCCTACCAGTATCTTT+CGG | + | chr4_4:7397318-7397337 | None:intergenic | 40.0% | |
| ! | CTTTTCGTGGAAACAAACGA+TGG | + | chr4_4:7397359-7397378 | None:intergenic | 40.0% |
| TTAAAGCTTGCAGAGTGTAG+TGG | - | chr4_4:7397552-7397571 | Msa0667170:CDS | 40.0% | |
| ! | CATGATCAACCGTTTTCTTC+CGG | - | chr4_4:7397593-7397612 | Msa0667170:CDS | 40.0% |
| CAAAGATGTGTCTGCAACGA+CGG | + | chr4_4:7397220-7397239 | None:intergenic | 45.0% | |
| ! | CCAGTATCTTTCGGTTTTCC+TGG | + | chr4_4:7397309-7397328 | None:intergenic | 45.0% |
| CCAGGAAAACCGAAAGATAC+TGG | - | chr4_4:7397306-7397325 | Msa0667170:CDS | 45.0% | |
| CCGAAAGATACTGGTAGGAA+TGG | - | chr4_4:7397315-7397334 | Msa0667170:CDS | 45.0% | |
| TCTTCTTCTCCAAAGGGATG+TGG | - | chr4_4:7397765-7397784 | Msa0667170:CDS | 45.0% | |
| GTCTCGAAACTTTCTTCCCT+TGG | - | chr4_4:7397787-7397806 | Msa0667170:CDS | 45.0% | |
| AGTCGGGACAGAAGAAACAA+CGG | - | chr4_4:7397907-7397926 | Msa0667170:CDS | 45.0% | |
| CTTTCTTCACGTTCGTGCAA+GGG | + | chr4_4:7397947-7397966 | None:intergenic | 45.0% | |
| TCTTTCTTCACGTTCGTGCA+AGG | + | chr4_4:7397948-7397967 | None:intergenic | 45.0% | |
| CAAAGATGTGTCTGCAACGA+CGG | + | chr4_4:7397220-7397239 | None:intergenic | 45.0% | |
| ! | CCAGTATCTTTCGGTTTTCC+TGG | + | chr4_4:7397309-7397328 | None:intergenic | 45.0% |
| CCAGGAAAACCGAAAGATAC+TGG | - | chr4_4:7397306-7397325 | Msa0667170:CDS | 45.0% | |
| CCGAAAGATACTGGTAGGAA+TGG | - | chr4_4:7397315-7397334 | Msa0667170:CDS | 45.0% | |
| TCTTCTTCTCCAAAGGGATG+TGG | - | chr4_4:7397765-7397784 | Msa0667170:CDS | 45.0% | |
| GTCTCGAAACTTTCTTCCCT+TGG | - | chr4_4:7397787-7397806 | Msa0667170:CDS | 45.0% | |
| AGTCGGGACAGAAGAAACAA+CGG | - | chr4_4:7397907-7397926 | Msa0667170:CDS | 45.0% | |
| CTTTCTTCACGTTCGTGCAA+GGG | + | chr4_4:7397947-7397966 | None:intergenic | 45.0% | |
| TCTTTCTTCACGTTCGTGCA+AGG | + | chr4_4:7397948-7397967 | None:intergenic | 45.0% | |
| CGTGGAAACAAACGATGGCA+GGG | + | chr4_4:7397354-7397373 | None:intergenic | 50.0% | |
| TCGTGGAAACAAACGATGGC+AGG | + | chr4_4:7397355-7397374 | None:intergenic | 50.0% | |
| ! | TGATCAGCCGCTTCTTTTCG+TGG | + | chr4_4:7397372-7397391 | None:intergenic | 50.0% |
| AGACGATGATGCAGCCAAAG+CGG | + | chr4_4:7397625-7397644 | None:intergenic | 50.0% | |
| GTTTCGAGACCACATCCCTT+TGG | + | chr4_4:7397777-7397796 | None:intergenic | 50.0% | |
| TTTGTCGTAGTCGTGGAGCA+TGG | + | chr4_4:7397858-7397877 | None:intergenic | 50.0% | |
| CTGCAGATTTGTCGTAGTCG+TGG | + | chr4_4:7397865-7397884 | None:intergenic | 50.0% | |
| CGTGGAAACAAACGATGGCA+GGG | + | chr4_4:7397354-7397373 | None:intergenic | 50.0% | |
| TCGTGGAAACAAACGATGGC+AGG | + | chr4_4:7397355-7397374 | None:intergenic | 50.0% | |
| ! | TGATCAGCCGCTTCTTTTCG+TGG | + | chr4_4:7397372-7397391 | None:intergenic | 50.0% |
| AGACGATGATGCAGCCAAAG+CGG | + | chr4_4:7397625-7397644 | None:intergenic | 50.0% | |
| GTTTCGAGACCACATCCCTT+TGG | + | chr4_4:7397777-7397796 | None:intergenic | 50.0% | |
| TTTGTCGTAGTCGTGGAGCA+TGG | + | chr4_4:7397858-7397877 | None:intergenic | 50.0% | |
| CTGCAGATTTGTCGTAGTCG+TGG | + | chr4_4:7397865-7397884 | None:intergenic | 50.0% | |
| GGAAACAAACGATGGCAGGG+TGG | + | chr4_4:7397351-7397370 | None:intergenic | 55.0% | |
| CGGTTGCGTCCGGAAGAAAA+CGG | + | chr4_4:7397605-7397624 | None:intergenic | 55.0% | |
| TCAGCGCAACTCGAGAGAGT+CGG | - | chr4_4:7397890-7397909 | Msa0667170:CDS | 55.0% | |
| GGAAACAAACGATGGCAGGG+TGG | + | chr4_4:7397351-7397370 | None:intergenic | 55.0% | |
| CGGTTGCGTCCGGAAGAAAA+CGG | + | chr4_4:7397605-7397624 | None:intergenic | 55.0% | |
| TCAGCGCAACTCGAGAGAGT+CGG | - | chr4_4:7397890-7397909 | Msa0667170:CDS | 55.0% | |
| TCTTCCGGACGCAACCGCTT+TGG | - | chr4_4:7397608-7397627 | Msa0667170:CDS | 60.0% | |
| GTCGTGGAGCATGGCAGTAC+AGG | + | chr4_4:7397849-7397868 | None:intergenic | 60.0% | |
| CAGCGCAACTCGAGAGAGTC+GGG | - | chr4_4:7397891-7397910 | Msa0667170:CDS | 60.0% | |
| TCTTCCGGACGCAACCGCTT+TGG | - | chr4_4:7397608-7397627 | Msa0667170:CDS | 60.0% | |
| GTCGTGGAGCATGGCAGTAC+AGG | + | chr4_4:7397849-7397868 | None:intergenic | 60.0% | |
| CAGCGCAACTCGAGAGAGTC+GGG | - | chr4_4:7397891-7397910 | Msa0667170:CDS | 60.0% | |
| GCAGCCAAAGCGGTTGCGTC+CGG | + | chr4_4:7397615-7397634 | None:intergenic | 65.0% | |
| GCAGCCAAAGCGGTTGCGTC+CGG | + | chr4_4:7397615-7397634 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_4 | gene | 7397195 | 7397974 | 7397195 | ID=Msa0667170;Name=Msa0667170 |
| chr4_4 | mRNA | 7397195 | 7397974 | 7397195 | ID=Msa0667170-mRNA-1;Parent=Msa0667170;Name=Msa0667170-mRNA-1;_AED=0.04;_eAED=0.04;_QI=0|-1|0|1|-1|1|1|0|259 |
| chr4_4 | exon | 7397195 | 7397974 | 7397195 | ID=Msa0667170-mRNA-1:exon:2444;Parent=Msa0667170-mRNA-1 |
| chr4_4 | CDS | 7397195 | 7397974 | 7397195 | ID=Msa0667170-mRNA-1:cds;Parent=Msa0667170-mRNA-1 |
| Gene Sequence |
| Protein sequence |
