Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0657970 | Msa0669770 | 0.800179 | 1.627366e-48 | -8.615850e-47 |
| Msa0669770 | Msa0885280 | 0.808204 | 3.473813e-50 | -8.615850e-47 |
| Msa0669770 | Msa1136090 | 0.807465 | 4.988631e-50 | -8.615850e-47 |
| Msa0406120 | Msa0669770 | 0.810759 | 9.824566e-51 | -8.615850e-47 |
| Msa0436780 | Msa0669770 | 0.820768 | 5.762053e-53 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 7 sgRNAs with CRISPR-Local
Find 17 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTAAAACTATCATTATTAAT+TGG | 0.248704 | 4_4:+11632559 | Msa0669770:three_prime_UTR |
| TCCTATATTGTCTGTCATAT+CGG | 0.369008 | 4_4:+11632520 | Msa0669770:three_prime_UTR |
| AAATGCACTCACTGCATATA+TGG | 0.373474 | 4_4:-11632611 | None:intergenic |
| GCCGATATGACAGACAATAT+AGG | 0.489408 | 4_4:-11632521 | None:intergenic |
| GACAGACAATATAGGAAAAG+AGG | 0.507170 | 4_4:-11632513 | None:intergenic |
| AGCAACTTTACTAAGCTCAT+AGG | 0.559777 | 4_4:-11632202 | None:intergenic |
| CTTTAGTATCGATTTCACGA+AGG | 0.668239 | 4_4:+11632362 | Msa0669770:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAAAACTATCATTATTAAT+TGG | + | chr4_4:11632559-11632578 | Msa0669770:three_prime_UTR | 10.0% |
| !! | AAAAAAAAAGGCAAATATTT+AGG | - | chr4_4:11632268-11632287 | None:intergenic | 15.0% |
| !! | TTGATCAGTACAAAAAAAAA+AGG | - | chr4_4:11632280-11632299 | None:intergenic | 20.0% |
| !! | TGCAAGTAAGTTCAATTTTA+TGG | + | chr4_4:11632225-11632244 | Msa0669770:intron | 25.0% |
| !! | AAAGGCAAATATTTAGGATA+AGG | - | chr4_4:11632262-11632281 | None:intergenic | 25.0% |
| ! | TTCAAAGCTTAAACTTTCTT+AGG | + | chr4_4:11632308-11632327 | Msa0669770:intron | 25.0% |
| ! | TCAAAGCTTAAACTTTCTTA+GGG | + | chr4_4:11632309-11632328 | Msa0669770:intron | 25.0% |
| !! | TCGTATATGTTTTTCTTTGT+AGG | + | chr4_4:11632339-11632358 | Msa0669770:intron | 25.0% |
| CAAAGCTTAAACTTTCTTAG+GGG | + | chr4_4:11632310-11632329 | Msa0669770:intron | 30.0% | |
| AAAGCTTAAACTTTCTTAGG+GGG | + | chr4_4:11632311-11632330 | Msa0669770:intron | 30.0% | |
| TCCTATATTGTCTGTCATAT+CGG | + | chr4_4:11632520-11632539 | Msa0669770:three_prime_UTR | 30.0% | |
| AGCAACTTTACTAAGCTCAT+AGG | - | chr4_4:11632205-11632224 | None:intergenic | 35.0% | |
| !! | CTTTAGTATCGATTTCACGA+AGG | + | chr4_4:11632362-11632381 | Msa0669770:CDS | 35.0% |
| ! | TGATTTTTGTTGCAAGAGCA+AGG | + | chr4_4:11632402-11632421 | Msa0669770:CDS | 35.0% |
| GACAGACAATATAGGAAAAG+AGG | - | chr4_4:11632516-11632535 | None:intergenic | 35.0% | |
| GCCGATATGACAGACAATAT+AGG | - | chr4_4:11632524-11632543 | None:intergenic | 40.0% | |
| !! | AAATATATAAAAAAAACTAA+AGG | - | chr4_4:11632471-11632490 | None:intergenic | 5.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_4 | gene | 11632186 | 11632622 | 11632186 | ID=Msa0669770;Name=Msa0669770 |
| chr4_4 | mRNA | 11632186 | 11632622 | 11632186 | ID=Msa0669770-mRNA-1;Parent=Msa0669770;Name=Msa0669770-mRNA-1;_AED=0.36;_eAED=0.43;_QI=0|0|0.5|1|1|0.5|2|148|48 |
| chr4_4 | exon | 11632186 | 11632218 | 11632186 | ID=Msa0669770-mRNA-1:exon:3528;Parent=Msa0669770-mRNA-1 |
| chr4_4 | exon | 11632361 | 11632622 | 11632361 | ID=Msa0669770-mRNA-1:exon:3529;Parent=Msa0669770-mRNA-1 |
| chr4_4 | CDS | 11632186 | 11632218 | 11632186 | ID=Msa0669770-mRNA-1:cds;Parent=Msa0669770-mRNA-1 |
| chr4_4 | CDS | 11632361 | 11632474 | 11632361 | ID=Msa0669770-mRNA-1:cds;Parent=Msa0669770-mRNA-1 |
| chr4_4 | three_prime_UTR | 11632475 | 11632622 | 11632475 | ID=Msa0669770-mRNA-1:three_prime_utr;Parent=Msa0669770-mRNA-1 |
| Gene Sequence |
| Protein sequence |
