AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0678420

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0678420 MtrunA17_Chr4g0023801 97.064 545 15 1 1 544 1 545 0.0 1090
Msa0678420 MtrunA17_Chr2g0280541 38.549 441 235 9 75 505 3 417 1.20e-96 301
Msa0678420 MtrunA17_Chr4g0037661 34.155 568 320 13 6 543 5 548 4.10e-94 297
Msa0678420 MtrunA17_Chr1g0163311 34.720 553 298 14 5 526 4 524 7.08e-93 293
Msa0678420 MtrunA17_Chr8g0374011 32.240 549 311 15 48 541 82 624 1.10e-65 225
Msa0678420 MtrunA17_Chr2g0303541 30.823 571 322 13 14 534 35 582 1.52e-63 219
Msa0678420 MtrunA17_Chr2g0320691 28.722 571 345 15 7 531 26 580 3.79e-61 213
Msa0678420 MtrunA17_Chr1g0199631 30.270 555 314 17 29 539 14 539 3.06e-55 195
Msa0678420 MtrunA17_Chr7g0245201 29.856 556 315 17 5 513 49 576 1.08e-54 195
Msa0678420 MtrunA17_Chr2g0285511 28.000 575 327 17 14 537 35 573 1.11e-54 194
Msa0678420 MtrunA17_Chr2g0279481 27.915 609 342 17 14 542 53 644 3.30e-54 194
Msa0678420 MtrunA17_Chr5g0401451 28.269 566 351 18 11 542 43 587 8.09e-52 187
Msa0678420 MtrunA17_Chr1g0181181 27.518 556 304 17 14 521 35 539 1.52e-51 185
Msa0678420 MtrunA17_Chr4g0050021 27.348 607 327 17 7 529 47 623 1.67e-51 187
Msa0678420 MtrunA17_Chr4g0010091 28.086 559 347 14 17 542 46 582 1.19e-50 183
Msa0678420 MtrunA17_Chr1g0195911 26.897 580 352 13 7 542 39 590 4.15e-50 182
Msa0678420 MtrunA17_Chr4g0053841 27.788 529 330 16 14 513 22 527 2.10e-46 171
Msa0678420 MtrunA17_Chr7g0268781 26.232 568 361 13 14 542 45 593 2.29e-46 172
Msa0678420 MtrunA17_Chr1g0207461 28.381 451 287 11 52 472 74 518 3.81e-45 168
Msa0678420 MtrunA17_Chr5g0412591 29.103 457 225 15 48 442 70 489 7.21e-45 167
Msa0678420 MtrunA17_Chr4g0076521 30.626 431 256 14 40 442 60 475 1.73e-44 166
Msa0678420 MtrunA17_Chr8g0382231 26.067 445 251 10 50 444 97 513 7.08e-43 162
Msa0678420 MtrunA17_Chr5g0401341 27.957 465 282 12 14 442 36 483 3.52e-41 157
Msa0678420 MtrunA17_Chr1g0150191 26.696 457 299 10 14 440 36 486 1.54e-39 152
Msa0678420 MtrunA17_Chr1g0191711 26.637 443 279 11 14 440 67 479 1.34e-38 149
Msa0678420 MtrunA17_Chr3g0108331 25.621 523 311 14 14 505 33 508 4.35e-38 147
Msa0678420 MtrunA17_Chr8g0367891 26.174 447 258 11 43 442 63 484 2.91e-35 139
Msa0678420 MtrunA17_Chr2g0290741 28.333 420 250 15 50 442 70 465 3.80e-35 139
Msa0678420 MtrunA17_Chr2g0332281 32.971 276 161 6 257 527 106 362 1.18e-33 132
Msa0678420 MtrunA17_Chr5g0432861 25.053 471 283 10 14 445 34 473 2.89e-33 134
Msa0678420 MtrunA17_Chr4g0003891 30.526 285 187 4 257 539 126 401 3.57e-32 129
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0678420 AT3G22104.1 47.601 542 238 7 7 544 7 506 3.74e-159 463
Msa0678420 AT3G22104.4 46.186 472 208 7 77 544 2 431 2.73e-126 377
Msa0678420 AT3G22104.2 46.186 472 208 7 77 544 2 431 2.73e-126 377
Msa0678420 AT3G22104.3 46.186 472 208 7 77 544 2 431 2.73e-126 377
Msa0678420 AT3G19850.1 34.982 566 324 15 6 543 5 554 4.21e-95 300
Msa0678420 AT1G50280.1 34.261 467 264 12 7 439 6 463 9.99e-72 238
Msa0678420 AT5G66560.4 32.959 534 307 12 43 531 61 588 1.60e-67 230
Msa0678420 AT5G66560.3 32.959 534 307 12 43 531 71 598 3.68e-67 229
Msa0678420 AT5G66560.1 32.959 534 307 12 43 531 100 627 4.75e-67 229
Msa0678420 AT1G50280.2 35.322 419 232 11 51 439 21 430 9.97e-67 224
Msa0678420 AT3G50840.1 29.476 553 333 13 7 537 19 536 1.48e-59 207
Msa0678420 AT3G08570.1 28.647 569 325 16 7 533 37 566 4.12e-59 207
Msa0678420 AT3G50840.2 30.392 510 299 12 50 537 25 500 4.12e-58 202
Msa0678420 AT3G50840.3 30.392 510 299 12 50 537 25 500 4.12e-58 202
Msa0678420 AT1G30440.1 30.017 573 338 15 14 534 35 596 9.90e-56 199
Msa0678420 AT5G03250.1 28.070 570 335 14 14 539 35 573 1.16e-54 194
Msa0678420 AT5G13600.1 28.924 567 320 19 15 537 36 563 5.45e-54 192
Msa0678420 AT3G08660.1 28.315 558 326 15 10 541 40 549 1.39e-53 191
Msa0678420 AT5G48800.1 29.264 557 352 16 7 542 44 579 4.44e-53 190
Msa0678420 AT3G08660.3 28.571 518 299 13 50 541 17 489 6.29e-53 188
Msa0678420 AT1G03010.1 28.454 608 338 18 14 544 15 602 2.77e-51 186
Msa0678420 AT1G03010.2 28.523 589 345 17 7 544 12 575 8.53e-51 184
Msa0678420 AT1G03010.3 28.523 589 345 17 7 544 38 601 1.25e-50 184
Msa0678420 AT3G44820.1 26.335 562 345 13 18 524 36 583 4.56e-50 182
Msa0678420 AT5G66560.2 35.659 387 206 11 43 392 100 480 5.76e-50 179
Msa0678420 AT5G67385.1 29.825 456 282 12 14 444 34 476 1.16e-49 181
Msa0678420 AT2G47860.1 27.915 566 327 17 7 524 40 572 1.25e-48 178
Msa0678420 AT5G67385.4 30.137 438 268 12 32 444 7 431 1.40e-48 177
Msa0678420 AT5G67385.5 30.137 438 268 12 32 444 7 431 1.40e-48 177
Msa0678420 AT5G67385.2 30.137 438 268 12 32 444 7 431 1.40e-48 177
Msa0678420 AT5G67385.3 30.137 438 268 12 32 444 7 431 1.40e-48 177
Msa0678420 AT2G47860.3 28.516 519 295 15 54 524 105 595 9.42e-47 174
Msa0678420 AT5G47800.3 29.167 432 252 13 43 440 63 474 1.69e-45 168
Msa0678420 AT5G47800.1 29.167 432 252 13 43 440 63 474 1.69e-45 168
Msa0678420 AT5G47800.4 29.167 432 252 13 43 440 63 474 1.69e-45 168
Msa0678420 AT5G47800.2 29.167 432 252 13 43 440 176 587 7.14e-45 168
Msa0678420 AT2G23050.1 28.643 398 248 9 52 440 78 448 9.97e-44 162
Msa0678420 AT3G49970.1 28.883 412 239 11 32 427 21 394 1.66e-42 159
Msa0678420 AT3G49970.2 28.883 412 239 11 32 427 21 394 1.66e-42 159
Msa0678420 AT2G14820.2 28.035 453 283 12 53 470 74 518 4.38e-42 160
Msa0678420 AT2G14820.1 28.035 453 283 12 53 470 74 518 4.38e-42 160
Msa0678420 AT5G03250.2 27.122 483 280 11 100 539 8 461 1.51e-41 156
Msa0678420 AT3G26490.1 29.412 459 256 13 52 456 75 519 1.09e-40 155
Msa0678420 AT3G26490.3 29.412 459 256 13 52 456 75 519 1.09e-40 155
Msa0678420 AT3G26490.4 29.412 459 256 13 52 456 75 519 1.09e-40 155
Msa0678420 AT2G30520.1 28.233 464 271 10 14 445 39 472 2.54e-39 152
Msa0678420 AT1G67900.5 27.051 451 259 11 52 442 69 509 1.30e-38 150
Msa0678420 AT1G67900.1 27.051 451 259 11 52 442 69 509 1.30e-38 150
Msa0678420 AT1G67900.3 27.051 451 259 11 52 442 69 509 1.30e-38 150
Msa0678420 AT1G67900.6 27.051 451 259 11 52 442 69 509 1.30e-38 150
Msa0678420 AT1G67900.2 27.051 451 259 11 52 442 69 509 1.30e-38 150
Msa0678420 AT1G67900.4 27.051 451 259 11 52 442 69 509 1.30e-38 150
Msa0678420 AT2G30520.2 28.539 438 252 9 40 445 16 424 1.15e-37 146
Msa0678420 AT2G30520.3 28.539 438 252 9 40 445 16 424 1.15e-37 146
Msa0678420 AT3G08660.2 28.889 405 240 11 10 393 40 417 6.08e-37 142
Msa0678420 AT5G67440.3 25.306 490 319 13 10 475 32 498 9.67e-36 141
Msa0678420 AT5G67440.1 25.306 490 319 13 10 475 32 498 9.67e-36 141
Msa0678420 AT4G31820.1 26.981 467 282 17 7 440 26 466 1.15e-35 141
Msa0678420 AT5G67440.2 25.478 471 304 13 29 475 14 461 5.16e-35 139
Msa0678420 AT4G37590.1 28.436 422 260 12 52 457 84 479 5.75e-35 139
Msa0678420 AT2G47860.5 26.638 473 277 12 98 524 6 454 9.14e-35 137
Msa0678420 AT2G47860.2 26.638 473 277 12 98 524 6 454 9.14e-35 137
Msa0678420 AT2G47860.4 28.372 430 239 13 7 392 40 444 3.43e-34 135
Msa0678420 AT4G31820.2 26.897 435 263 15 35 440 5 413 7.16e-34 135
Msa0678420 AT2G47860.6 29.243 383 207 11 54 392 105 467 1.34e-32 130
Msa0678420 AT2G23050.2 27.374 358 225 8 92 440 1 332 5.46e-32 127
Msa0678420 AT3G26490.2 29.114 395 214 12 52 392 75 457 8.93e-32 129
Msa0678420 AT5G64330.1 31.804 327 177 5 258 542 388 710 3.18e-31 129
Msa0678420 AT5G64330.1 24.590 244 140 7 14 229 61 288 4.26e-15 79.0
Msa0678420 AT1G52770.1 31.507 292 176 6 257 539 116 392 6.41e-31 125
Msa0678420 AT3G15570.1 30.000 290 175 4 257 536 121 392 3.81e-30 123
Msa0678420 AT5G64330.2 34.711 242 117 4 258 462 388 625 3.62e-29 122
Msa0678420 AT5G64330.2 24.590 244 140 7 14 229 61 288 2.86e-15 79.3
Msa0678420 AT5G64330.3 34.118 255 126 5 245 462 317 566 4.10e-29 122
Msa0678420 AT5G64330.3 25.714 210 113 6 48 229 35 229 1.71e-14 76.6
Msa0678420 AT5G48130.1 26.961 408 264 12 49 440 78 467 6.17e-29 121
Msa0678420 AT5G10250.2 28.082 292 173 6 260 536 311 580 7.82e-27 115
Msa0678420 AT5G10250.2 34.400 125 79 3 7 131 53 174 2.82e-13 73.2
Msa0678420 AT5G10250.1 28.114 281 172 5 260 536 311 565 2.93e-25 110
Msa0678420 AT5G10250.1 34.400 125 79 3 7 131 53 174 3.15e-13 72.8
Msa0678420 AT3G03510.1 25.954 393 248 13 60 445 8 364 6.98e-21 95.9
Msa0678420 AT5G17580.1 23.736 455 314 15 7 445 8 445 5.33e-17 84.7
Msa0678420 AT5G17580.2 23.736 455 314 15 7 445 23 460 6.43e-17 84.3

Find 113 sgRNAs with CRISPR-Local

Find 199 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTTCTTTCAAAACATTATT+TGG 0.102995 4_4:+34131040 Msa0678420:CDS
AGCAAATTGGCTAAAGTATT+TGG 0.117951 4_4:+34130347 Msa0678420:CDS
GTCAATGCTTTCGAGTAAAT+TGG 0.197083 4_4:+34130889 Msa0678420:CDS
CGAAAGTGTTTCTAACTTAC+TGG 0.232347 4_4:+34130547 Msa0678420:CDS
CGGTTCAAGGGATTAGTTAT+TGG 0.270197 4_4:+34130585 Msa0678420:CDS
CGAATGACAGTTCAGGGATT+AGG 0.302390 4_4:+34130753 Msa0678420:CDS
ATCATTGTATGGAGTTGAAA+TGG 0.302995 4_4:-34132082 None:intergenic
AAACATTATTTGGGATTCTT+CGG 0.325788 4_4:+34131050 Msa0678420:CDS
ATACAACACAACTTGTTCAT+TGG 0.346804 4_4:-34132154 None:intergenic
TACTCAATACTCGAGCAAAT+TGG 0.347083 4_4:+34130334 Msa0678420:CDS
AACATTATTTGGGATTCTTC+GGG 0.354248 4_4:+34131051 Msa0678420:CDS
CAAGAATCCCTAGCAGAATC+TGG 0.359396 4_4:-34131373 None:intergenic
TTTCTTTCAAAACATTATTT+GGG 0.363196 4_4:+34131041 Msa0678420:CDS
ATTGTACAGTCACTAAATAA+TGG 0.370741 4_4:+34132485 Msa0678420:three_prime_UTR
TGTAGTTGGGAGGCTGGTTT+CGG 0.375981 4_4:+34130697 Msa0678420:CDS
AGTTGTTCGAATGACAGTTC+AGG 0.376305 4_4:+34130746 Msa0678420:CDS
AATCTTGAAGTGGATGTAAA+TGG 0.387252 4_4:+34129138 Msa0678420:CDS
AGGAATGCAGTGGAGGGTTA+TGG 0.389720 4_4:+34132230 Msa0678420:CDS
GATTATGATCGGTTCACAAT+TGG 0.390206 4_4:+34131120 Msa0678420:CDS
TTCAAGTGGTTCTTCTCAAA+AGG 0.390409 4_4:+34132116 Msa0678420:CDS
GAAAGACAAAACCAAGGAAA+AGG 0.412154 4_4:-34128944 None:intergenic
GGATGCTGTAGTTGGGAGGC+TGG 0.417675 4_4:+34130691 Msa0678420:CDS
CTGCTTAATTGATGTTGGTT+GGG 0.427402 4_4:+34132444 Msa0678420:three_prime_UTR
GCGTTCCTGCGGCAAGGAAC+TGG 0.427410 4_4:+34131232 Msa0678420:CDS
AGGTTGAATATCGAAATTTC+CGG 0.430532 4_4:-34132178 None:intergenic
TGGATCATTATGTTATAAGT+AGG 0.433791 4_4:+34130909 Msa0678420:CDS
AATGAGGATGGAAGAGGAAA+AGG 0.438792 4_4:-34129036 None:intergenic
AGAAACTTTCAAGGTGGATA+AGG 0.444153 4_4:+34129164 Msa0678420:CDS
TTTCTAGTTATCATGGATGA+AGG 0.445584 4_4:+34129108 Msa0678420:exon
GAAGTGCAAAATCATTGAAA+TGG 0.446426 4_4:+34130982 Msa0678420:CDS
TCTGATGCTAGAGAAGTGTT+TGG 0.449847 4_4:+34130670 Msa0678420:CDS
AAAGCATCTGGTCATAGTTA+TGG 0.455369 4_4:+34132309 Msa0678420:CDS
GGAAAAGGGTTGTGGTCAAT+TGG 0.456525 4_4:-34128929 None:intergenic
TTACCAACATGATAACCATC+TGG 0.456686 4_4:-34130865 None:intergenic
CCACACAAAGAATCATTGTA+TGG 0.462883 4_4:-34132093 None:intergenic
ATGACAAAATCAAAAGCATC+TGG 0.463126 4_4:+34132297 Msa0678420:CDS
GGGTTATAGTTGTGTTGGAA+TGG 0.469151 4_4:-34128882 None:intergenic
TCATTGTATGGAGTTGAAAT+GGG 0.472028 4_4:-34132081 None:intergenic
ACTGCTTAATTGATGTTGGT+TGG 0.472912 4_4:+34132443 Msa0678420:three_prime_UTR
TTCTGTTATAACAATGGAAA+AGG 0.474857 4_4:+34130455 Msa0678420:CDS
TCAAAACCTTCTGCACCTCC+TGG 0.479630 4_4:-34130419 None:intergenic
ATATAAGTAGTTTATTCCAT+AGG 0.480644 4_4:-34131179 None:intergenic
AGTGTTTGGATGCTGTAGTT+GGG 0.482062 4_4:+34130684 Msa0678420:CDS
AAGTGTTTGGATGCTGTAGT+TGG 0.484528 4_4:+34130683 Msa0678420:CDS
GGAATTTCGTGTTCTTTGAA+AGG 0.487487 4_4:-34131984 None:intergenic
CGGTTGCTTCCGTTCGGATG+AGG 0.490154 4_4:+34131257 Msa0678420:CDS
AAGAGTCATGAACATAGCTT+TGG 0.491223 4_4:-34132342 None:intergenic
TTGTACTGCTTAATTGATGT+TGG 0.492387 4_4:+34132439 Msa0678420:three_prime_UTR
TAAATGTAGCAGGAATAAGT+TGG 0.492750 4_4:+34131096 Msa0678420:CDS
CTGGTGCGGTTGCTTCCGTT+CGG 0.496027 4_4:+34131251 Msa0678420:CDS
TATAAGTAGTTTATTCCATA+GGG 0.496705 4_4:-34131178 None:intergenic
GTTGTTCGAATGACAGTTCA+GGG 0.497978 4_4:+34130747 Msa0678420:CDS
TTGCTAGTTCCATCTCCCTA+TGG 0.504707 4_4:+34131163 Msa0678420:CDS
GGAAAATCTAGTGGTGGAAG+TGG 0.507767 4_4:+34130368 Msa0678420:CDS
CTGCACCTCCTGGAAAATCA+TGG 0.509317 4_4:-34130409 None:intergenic
TGAACATAAGTAAATGTAGC+AGG 0.512311 4_4:+34131086 Msa0678420:CDS
TCAAGTGGTTCTTCTCAAAA+GGG 0.512620 4_4:+34132117 Msa0678420:CDS
AAAGACAAAACCAAGGAAAA+GGG 0.518371 4_4:-34128943 None:intergenic
CAGCTCTTCCAGATTCTGCT+AGG 0.520217 4_4:+34131365 Msa0678420:CDS
CCATACAATGATTCTTTGTG+TGG 0.523116 4_4:+34132093 Msa0678420:CDS
AAGGGATTAGTTATTGGTCA+TGG 0.523562 4_4:+34130591 Msa0678420:CDS
GAGACAGAAGTAGTGCGGTT+GGG 0.532780 4_4:-34128902 None:intergenic
TCGTGTTCTTTGAAAGGTGT+AGG 0.535760 4_4:-34131978 None:intergenic
TACCAACATGATAACCATCT+GGG 0.536405 4_4:-34130864 None:intergenic
CGGTTGGGTTATAGTTGTGT+TGG 0.537400 4_4:-34128887 None:intergenic
ACAATTGGATCAAGTAACAT+TGG 0.540829 4_4:+34131135 Msa0678420:CDS
GTTTCTTCTGTATTATCAGA+AGG 0.541194 4_4:+34130931 Msa0678420:CDS
CCGCACCAGTTCCTTGCCGC+AGG 0.541269 4_4:-34131237 None:intergenic
CGAATTTCACTCGTTCGACA+TGG 0.541979 4_4:+34130813 Msa0678420:CDS
TAGTTTATTCCATAGGGAGA+TGG 0.542308 4_4:-34131172 None:intergenic
TTAAGATTCTGTTATAACAA+TGG 0.544300 4_4:+34130449 Msa0678420:CDS
TCCCCAGATGGTTATCATGT+TGG 0.544832 4_4:+34130862 Msa0678420:CDS
AAATGGAGAAGAAACTTTCA+AGG 0.545300 4_4:+34129155 Msa0678420:CDS
AGAGACAGAAGTAGTGCGGT+TGG 0.558217 4_4:-34128903 None:intergenic
ACATGAAGTGATATTCTAAG+AGG 0.561631 4_4:-34128994 None:intergenic
CGTTCGTCTTGTGAGAGTTG+AGG 0.564013 4_4:-34131904 None:intergenic
AAAACCAAGGAAAAGGGTTG+TGG 0.564062 4_4:-34128937 None:intergenic
AGCTCTTCCAGATTCTGCTA+GGG 0.564119 4_4:+34131366 Msa0678420:CDS
TTGAAAGCGTTCCTGCGGCA+AGG 0.567464 4_4:+34131226 Msa0678420:CDS
AATAAGTTGGAGATTATGAT+CGG 0.569991 4_4:+34131109 Msa0678420:CDS
ATTTGCAAGGAATGCAGTGG+AGG 0.572628 4_4:+34132223 Msa0678420:CDS
GATTCTTTGTGTGGTTCAAG+TGG 0.575011 4_4:+34132102 Msa0678420:CDS
TGATGAGCTTTACCATGCAA+TGG 0.576782 4_4:+34131396 Msa0678420:CDS
CGCGCGAGTAACAGATTCGA+AGG 0.577244 4_4:-34130488 None:intergenic
GAACGGATAAAGATATGTTG+TGG 0.585661 4_4:+34131922 Msa0678420:CDS
ATATGCATTACGAAATAGAC+CGG 0.586731 4_4:+34131011 Msa0678420:CDS
TTCGAACAACTCGAGGGACA+CGG 0.589174 4_4:-34130734 None:intergenic
AAAGTATTTGGAAAATCTAG+TGG 0.598023 4_4:+34130359 Msa0678420:CDS
GCGTTGTGCTGCTGAATACA+TGG 0.598711 4_4:+34130508 Msa0678420:CDS
GAACAAGTTGTGTTGTATGC+CGG 0.605824 4_4:+34132159 Msa0678420:CDS
TTACATATAATATTGTGTGG+AGG 0.609904 4_4:-34128798 None:intergenic
CTCAACTCTCACAAGACGAA+CGG 0.610795 4_4:+34131905 Msa0678420:CDS
AACATTTGCAAGGAATGCAG+TGG 0.611430 4_4:+34132220 Msa0678420:CDS
CGAGATACATGTCCATTGCA+TGG 0.612034 4_4:-34131408 None:intergenic
AAAATGAAAGAACATTTGCA+AGG 0.616420 4_4:+34132210 Msa0678420:CDS
TTTGCAAGGAATGCAGTGGA+GGG 0.620645 4_4:+34132224 Msa0678420:CDS
GTTTGGATGCTGTAGTTGGG+AGG 0.620714 4_4:+34130687 Msa0678420:CDS
AGGTTTCTGCAATCTTGAAG+TGG 0.626848 4_4:+34129128 Msa0678420:CDS
ATTTCACTCGTTCGACATGG+TGG 0.628952 4_4:+34130816 Msa0678420:CDS
AGCAACTTTCCTCATCCGAA+CGG 0.641724 4_4:-34131266 None:intergenic
GTGTTACATATAATATTGTG+TGG 0.649183 4_4:-34128801 None:intergenic
TGCAATGGACATGTATCTCG+AGG 0.652835 4_4:+34131411 Msa0678420:CDS
GTATTTGGAAAATCTAGTGG+TGG 0.653932 4_4:+34130362 Msa0678420:CDS
AAGACAGAAAGACAAAACCA+AGG 0.657843 4_4:-34128950 None:intergenic
CCTGCGGCAAGGAACTGGTG+CGG 0.658037 4_4:+34131237 Msa0678420:CDS
ACTGTCATTCGAACAACTCG+AGG 0.659603 4_4:-34130741 None:intergenic
AATGGATACAACAAGGACAA+AGG 0.667374 4_4:-34128864 None:intergenic
GAAAGGTGTAGGCATGCTTG+AGG 0.672792 4_4:-34131967 None:intergenic
ACCAACATGATAACCATCTG+GGG 0.676918 4_4:-34130863 None:intergenic
CTGTCATTCGAACAACTCGA+GGG 0.677499 4_4:-34130740 None:intergenic
TGGAGAAGAAACTTTCAAGG+TGG 0.678777 4_4:+34129158 Msa0678420:CDS
GTGCAGAGACAGAAGTAGTG+CGG 0.703525 4_4:-34128907 None:intergenic
GTGTTGGAATGGATACAACA+AGG 0.725933 4_4:-34128871 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTTTGTATGTTTTAAT+AGG + chr4_4:34129477-34129496 Msa0678420:intron 10.0%
!! TAAACTAAAATTGTTTAAAT+TGG + chr4_4:34130292-34130311 Msa0678420:intron 10.0%
!!! TCAAAATTTAAAATTCAAAA+TGG - chr4_4:34131451-34131470 None:intergenic 10.0%
!! GAAATTATTATTAATAAAGA+TGG - chr4_4:34131864-34131883 None:intergenic 10.0%
!!! ATTTTTTTTTCTAGTTATCA+TGG + chr4_4:34129101-34129120 Msa0678420:exon 15.0%
!! TTTATATTATAGGATTTACA+AGG + chr4_4:34129522-34129541 Msa0678420:intron 15.0%
!!! CTTTAATTTAGAAATGATTA+TGG + chr4_4:34129610-34129629 Msa0678420:intron 15.0%
!! AGTGAAATTAAAAAACTAAT+AGG - chr4_4:34129657-34129676 None:intergenic 15.0%
!! AAAAAATAATAAATCAAGCA+TGG - chr4_4:34130014-34130033 None:intergenic 15.0%
!! TATAAAGATAGGAAAATATA+TGG - chr4_4:34130155-34130174 None:intergenic 15.0%
!! TTTCTTTCAAAACATTATTT+GGG + chr4_4:34131041-34131060 Msa0678420:CDS 15.0%
!!! TTTAAAAACTTCAAAGTTTT+TGG + chr4_4:34131333-34131352 Msa0678420:CDS 15.0%
!!! ATAAAGATTTCTTACAATTT+TGG - chr4_4:34131676-34131695 None:intergenic 15.0%
!! GTACTAGTAGTTTATATTAT+AGG + chr4_4:34129512-34129531 Msa0678420:intron 20.0%
!! ATTTAGGTGCAAATTAATTA+TGG - chr4_4:34129724-34129743 None:intergenic 20.0%
!!! TTTTTGTTAACATTTTTGAC+AGG + chr4_4:34129943-34129962 Msa0678420:intron 20.0%
!!! TTTCTGTGCTTTAATTATTT+TGG + chr4_4:34130110-34130129 Msa0678420:intron 20.0%
!!! ATGTTTTTGGTAAGTTATTT+TGG + chr4_4:34130213-34130232 Msa0678420:intron 20.0%
!! TTAAGATTCTGTTATAACAA+TGG + chr4_4:34130449-34130468 Msa0678420:CDS 20.0%
!! GTTTCTTTCAAAACATTATT+TGG + chr4_4:34131040-34131059 Msa0678420:CDS 20.0%
!! TATAAGTAGTTTATTCCATA+GGG - chr4_4:34131181-34131200 None:intergenic 20.0%
!! ATATAAGTAGTTTATTCCAT+AGG - chr4_4:34131182-34131201 None:intergenic 20.0%
!!! AACTTTGAAGTTTTTAAACA+AGG - chr4_4:34131331-34131350 None:intergenic 20.0%
!!! AATTTTGAAGTCTATAACTT+TGG + chr4_4:34131463-34131482 Msa0678420:intron 20.0%
!!! TTTCTTACAATTTTGGATAA+GGG - chr4_4:34131669-34131688 None:intergenic 20.0%
!!! ATTTCTTACAATTTTGGATA+AGG - chr4_4:34131670-34131689 None:intergenic 20.0%
!!! TTTTCACATTTGTATAGTAA+AGG + chr4_4:34132399-34132418 Msa0678420:three_prime_UTR 20.0%
! GTGTTACATATAATATTGTG+TGG - chr4_4:34128804-34128823 None:intergenic 25.0%
!!! TTTTTTGTAAAGTGTATGTC+TGG + chr4_4:34129190-34129209 Msa0678420:intron 25.0%
! TACAATCATAAAGTTACCAA+GGG - chr4_4:34129689-34129708 None:intergenic 25.0%
! CAAAAACAAACAAAATACGA+AGG - chr4_4:34129929-34129948 None:intergenic 25.0%
! GACAATAAATTGTAGAATGA+GGG - chr4_4:34130063-34130082 None:intergenic 25.0%
! AGACAATAAATTGTAGAATG+AGG - chr4_4:34130064-34130083 None:intergenic 25.0%
!!! CTCTAGTTATTTCATGTTTT+TGG + chr4_4:34130200-34130219 Msa0678420:intron 25.0%
!! AAAGTATTTGGAAAATCTAG+TGG + chr4_4:34130359-34130378 Msa0678420:CDS 25.0%
! TTCTGTTATAACAATGGAAA+AGG + chr4_4:34130455-34130474 Msa0678420:CDS 25.0%
! TGGATCATTATGTTATAAGT+AGG + chr4_4:34130909-34130928 Msa0678420:CDS 25.0%
!! AAACATTATTTGGGATTCTT+CGG + chr4_4:34131050-34131069 Msa0678420:CDS 25.0%
!! AATAAGTTGGAGATTATGAT+CGG + chr4_4:34131109-34131128 Msa0678420:CDS 25.0%
! TAATCAAGTAGTTAACTACA+TGG - chr4_4:34131489-34131508 None:intergenic 25.0%
! AATCTTTATCTTCTACTCAT+TGG + chr4_4:34131687-34131706 Msa0678420:intron 25.0%
! TAATCACATCTACATCAAAA+CGG + chr4_4:34131818-34131837 Msa0678420:intron 25.0%
! ATTAATAAAGATGGCGTTAA+TGG - chr4_4:34131855-34131874 None:intergenic 25.0%
! ATAATTTCCTCTTCTGTTAT+AGG + chr4_4:34131876-34131895 Msa0678420:intron 25.0%
!! TCTTTCATTTTCTCATTATC+AGG - chr4_4:34132201-34132220 None:intergenic 25.0%
! AAAATGAAAGAACATTTGCA+AGG + chr4_4:34132210-34132229 Msa0678420:CDS 25.0%
! ATTGTACAGTCACTAAATAA+TGG + chr4_4:34132485-34132504 Msa0678420:three_prime_UTR 25.0%
AAAGACAAAACCAAGGAAAA+GGG - chr4_4:34128946-34128965 None:intergenic 30.0%
ACATGAAGTGATATTCTAAG+AGG - chr4_4:34128997-34129016 None:intergenic 30.0%
TGAGAAAACAAAAATGAGGA+TGG - chr4_4:34129051-34129070 None:intergenic 30.0%
CAGTTGAGAAAACAAAAATG+AGG - chr4_4:34129055-34129074 None:intergenic 30.0%
!! TTTCTAGTTATCATGGATGA+AGG + chr4_4:34129108-34129127 Msa0678420:exon 30.0%
AATCTTGAAGTGGATGTAAA+TGG + chr4_4:34129138-34129157 Msa0678420:CDS 30.0%
AAATGGAGAAGAAACTTTCA+AGG + chr4_4:34129155-34129174 Msa0678420:CDS 30.0%
! GATTTACAAGGTTTGTCTTT+TGG + chr4_4:34129534-34129553 Msa0678420:intron 30.0%
CTACAATCATAAAGTTACCA+AGG - chr4_4:34129690-34129709 None:intergenic 30.0%
AGGTGCAAATTAATTATGGA+TGG - chr4_4:34129720-34129739 None:intergenic 30.0%
!! AAAAAAGTGTCTTGTGTTTG+TGG - chr4_4:34129814-34129833 None:intergenic 30.0%
! ATGGTCTTCTAATGCTTTTA+TGG + chr4_4:34129899-34129918 Msa0678420:intron 30.0%
! GAAACCAAAAACATTGATTG+AGG - chr4_4:34130094-34130113 None:intergenic 30.0%
AAGTAAGCAGGTATAAAGAT+AGG - chr4_4:34130166-34130185 None:intergenic 30.0%
! AGCAAATTGGCTAAAGTATT+TGG + chr4_4:34130347-34130366 Msa0678420:CDS 30.0%
! GTAATATTCCATGATTTTCC+AGG + chr4_4:34130401-34130420 Msa0678420:CDS 30.0%
ACTGGATCAAACTGAAAAAT+CGG + chr4_4:34130565-34130584 Msa0678420:CDS 30.0%
! AAACTGAAAAATCGGTTCAA+GGG + chr4_4:34130573-34130592 Msa0678420:CDS 30.0%
GTTTCTTCTGTATTATCAGA+AGG + chr4_4:34130931-34130950 Msa0678420:CDS 30.0%
GAAGTGCAAAATCATTGAAA+TGG + chr4_4:34130982-34131001 Msa0678420:CDS 30.0%
ATATGCATTACGAAATAGAC+CGG + chr4_4:34131011-34131030 Msa0678420:CDS 30.0%
!! TTTTGAAAGAAACAGAACTC+CGG - chr4_4:34131033-34131052 None:intergenic 30.0%
! AACATTATTTGGGATTCTTC+GGG + chr4_4:34131051-34131070 Msa0678420:CDS 30.0%
TGAACATAAGTAAATGTAGC+AGG + chr4_4:34131086-34131105 Msa0678420:CDS 30.0%
TAAATGTAGCAGGAATAAGT+TGG + chr4_4:34131096-34131115 Msa0678420:CDS 30.0%
ACAATTGGATCAAGTAACAT+TGG + chr4_4:34131135-34131154 Msa0678420:CDS 30.0%
CTTTCAAAAATCTCAGAACA+AGG - chr4_4:34131213-34131232 None:intergenic 30.0%
! GAAGTTTTTAAACAAGGATC+AGG - chr4_4:34131325-34131344 None:intergenic 30.0%
!! AAAAAACTAATCGGCTTGTA+AGG - chr4_4:34131589-34131608 None:intergenic 30.0%
!!! TACAAGCCGATTAGTTTTTT+AGG + chr4_4:34131589-34131608 Msa0678420:intron 30.0%
!!! ACAAGCCGATTAGTTTTTTA+GGG + chr4_4:34131590-34131609 Msa0678420:intron 30.0%
ACATCAAAACGGTTGTAATA+CGG + chr4_4:34131829-34131848 Msa0678420:intron 30.0%
TCATTGTATGGAGTTGAAAT+GGG - chr4_4:34132084-34132103 None:intergenic 30.0%
ATCATTGTATGGAGTTGAAA+TGG - chr4_4:34132085-34132104 None:intergenic 30.0%
! ATACAACACAACTTGTTCAT+TGG - chr4_4:34132157-34132176 None:intergenic 30.0%
AGGTTGAATATCGAAATTTC+CGG - chr4_4:34132181-34132200 None:intergenic 30.0%
!! ATGACAAAATCAAAAGCATC+TGG + chr4_4:34132297-34132316 Msa0678420:CDS 30.0%
TTGTACTGCTTAATTGATGT+TGG + chr4_4:34132439-34132458 Msa0678420:three_prime_UTR 30.0%
AATGGATACAACAAGGACAA+AGG - chr4_4:34128867-34128886 None:intergenic 35.0%
GAAAGACAAAACCAAGGAAA+AGG - chr4_4:34128947-34128966 None:intergenic 35.0%
AAGACAGAAAGACAAAACCA+AGG - chr4_4:34128953-34128972 None:intergenic 35.0%
AACAAAAATGAGGATGGAAG+AGG - chr4_4:34129045-34129064 None:intergenic 35.0%
! TGTTTTCTCAACTGAACAGA+AGG + chr4_4:34129061-34129080 Msa0678420:five_prime_UTR 35.0%
AGAAACTTTCAAGGTGGATA+AGG + chr4_4:34129164-34129183 Msa0678420:CDS 35.0%
CAAGTGTATGTCTGATATCA+TGG + chr4_4:34129266-34129285 Msa0678420:intron 35.0%
AAGGAAAATCATTGCAAGCA+TGG - chr4_4:34129776-34129795 None:intergenic 35.0%
! AACATTTTTGACAGGATCTG+AGG + chr4_4:34129951-34129970 Msa0678420:intron 35.0%
! ACATTTTTGACAGGATCTGA+GGG + chr4_4:34129952-34129971 Msa0678420:intron 35.0%
! ACGACCTCAATCAATGTTTT+TGG + chr4_4:34130087-34130106 Msa0678420:intron 35.0%
CATACACAAGAAAAGTAAGC+AGG - chr4_4:34130178-34130197 None:intergenic 35.0%
TACTCAATACTCGAGCAAAT+TGG + chr4_4:34130334-34130353 Msa0678420:CDS 35.0%
GTATTTGGAAAATCTAGTGG+TGG + chr4_4:34130362-34130381 Msa0678420:CDS 35.0%
! ATATTCCATGATTTTCCAGG+AGG + chr4_4:34130404-34130423 Msa0678420:CDS 35.0%
!! CGAAAGTGTTTCTAACTTAC+TGG + chr4_4:34130547-34130566 Msa0678420:CDS 35.0%
! CAAACTGAAAAATCGGTTCA+AGG + chr4_4:34130572-34130591 Msa0678420:CDS 35.0%
!! AAGGGATTAGTTATTGGTCA+TGG + chr4_4:34130591-34130610 Msa0678420:CDS 35.0%
TACCAACATGATAACCATCT+GGG - chr4_4:34130867-34130886 None:intergenic 35.0%
TTACCAACATGATAACCATC+TGG - chr4_4:34130868-34130887 None:intergenic 35.0%
! GTCAATGCTTTCGAGTAAAT+TGG + chr4_4:34130889-34130908 Msa0678420:CDS 35.0%
! GATTATGATCGGTTCACAAT+TGG + chr4_4:34131120-34131139 Msa0678420:CDS 35.0%
TAGTTTATTCCATAGGGAGA+TGG - chr4_4:34131175-34131194 None:intergenic 35.0%
!! TTGAGAGTCGACTTGAAATA+TGG + chr4_4:34131528-34131547 Msa0678420:intron 35.0%
CACGACCCTAAAAAACTAAT+CGG - chr4_4:34131598-34131617 None:intergenic 35.0%
!!! TAGTTTTTTAGGGTCGTGTT+AGG + chr4_4:34131600-34131619 Msa0678420:intron 35.0%
!!! CAATTTTGGATAAGGGCATT+CGG - chr4_4:34131662-34131681 None:intergenic 35.0%
GTAAAAAATCGTCACACTGT+TGG - chr4_4:34131752-34131771 None:intergenic 35.0%
GAACGGATAAAGATATGTTG+TGG + chr4_4:34131922-34131941 Msa0678420:CDS 35.0%
GGAATTTCGTGTTCTTTGAA+AGG - chr4_4:34131987-34132006 None:intergenic 35.0%
!!! CTTGAACTGCTGATTTTGAT+GGG - chr4_4:34132008-34132027 None:intergenic 35.0%
CCACACAAAGAATCATTGTA+TGG - chr4_4:34132096-34132115 None:intergenic 35.0%
CCATACAATGATTCTTTGTG+TGG + chr4_4:34132093-34132112 Msa0678420:CDS 35.0%
TTCAAGTGGTTCTTCTCAAA+AGG + chr4_4:34132116-34132135 Msa0678420:CDS 35.0%
TCAAGTGGTTCTTCTCAAAA+GGG + chr4_4:34132117-34132136 Msa0678420:CDS 35.0%
! AAAGCATCTGGTCATAGTTA+TGG + chr4_4:34132309-34132328 Msa0678420:CDS 35.0%
AAGAGTCATGAACATAGCTT+TGG - chr4_4:34132345-34132364 None:intergenic 35.0%
ACTGCTTAATTGATGTTGGT+TGG + chr4_4:34132443-34132462 Msa0678420:three_prime_UTR 35.0%
! CTGCTTAATTGATGTTGGTT+GGG + chr4_4:34132444-34132463 Msa0678420:three_prime_UTR 35.0%
GTGTTGGAATGGATACAACA+AGG - chr4_4:34128874-34128893 None:intergenic 40.0%
GGGTTATAGTTGTGTTGGAA+TGG - chr4_4:34128885-34128904 None:intergenic 40.0%
AAAACCAAGGAAAAGGGTTG+TGG - chr4_4:34128940-34128959 None:intergenic 40.0%
AATGAGGATGGAAGAGGAAA+AGG - chr4_4:34129039-34129058 None:intergenic 40.0%
AGGTTTCTGCAATCTTGAAG+TGG + chr4_4:34129128-34129147 Msa0678420:CDS 40.0%
TGGAGAAGAAACTTTCAAGG+TGG + chr4_4:34129158-34129177 Msa0678420:CDS 40.0%
AAAGTGTATGTCTGGTAGCA+CGG + chr4_4:34129198-34129217 Msa0678420:intron 40.0%
AACCAGTATGACTTTGAGTC+TGG + chr4_4:34129310-34129329 Msa0678420:intron 40.0%
TTCCAGACTCAAAGTCATAC+TGG - chr4_4:34129315-34129334 None:intergenic 40.0%
!! TTTCACTTTCTAGCTACCCT+TGG + chr4_4:34129670-34129689 Msa0678420:intron 40.0%
CACCTAAATAGCAGTGAAGT+GGG + chr4_4:34129735-34129754 Msa0678420:intron 40.0%
ACCTAAATAGCAGTGAAGTG+GGG + chr4_4:34129736-34129755 Msa0678420:intron 40.0%
GTGGTTAGTGAAAGTAGTGA+AGG - chr4_4:34129795-34129814 None:intergenic 40.0%
CACATTTAATGCTGCTGCTA+TGG + chr4_4:34129880-34129899 Msa0678420:intron 40.0%
!! TAGAATGAGGGAGAGTATCA+AGG - chr4_4:34130051-34130070 None:intergenic 40.0%
!! CGGTTCAAGGGATTAGTTAT+TGG + chr4_4:34130585-34130604 Msa0678420:CDS 40.0%
!! TCTGATGCTAGAGAAGTGTT+TGG + chr4_4:34130670-34130689 Msa0678420:CDS 40.0%
AAGTGTTTGGATGCTGTAGT+TGG + chr4_4:34130683-34130702 Msa0678420:CDS 40.0%
AGTGTTTGGATGCTGTAGTT+GGG + chr4_4:34130684-34130703 Msa0678420:CDS 40.0%
AGTTGTTCGAATGACAGTTC+AGG + chr4_4:34130746-34130765 Msa0678420:CDS 40.0%
GTTGTTCGAATGACAGTTCA+GGG + chr4_4:34130747-34130766 Msa0678420:CDS 40.0%
ACCAACATGATAACCATCTG+GGG - chr4_4:34130866-34130885 None:intergenic 40.0%
!!! GATTTTTGAAAGCGTTCCTG+CGG + chr4_4:34131221-34131240 Msa0678420:CDS 40.0%
TGATGAGCTTTACCATGCAA+TGG + chr4_4:34131396-34131415 Msa0678420:CDS 40.0%
GGATGTACCTATAACAGAAG+AGG - chr4_4:34131886-34131905 None:intergenic 40.0%
! TCGTGTTCTTTGAAAGGTGT+AGG - chr4_4:34131981-34132000 None:intergenic 40.0%
!!! GCTTGAACTGCTGATTTTGA+TGG - chr4_4:34132009-34132028 None:intergenic 40.0%
GATTCTTTGTGTGGTTCAAG+TGG + chr4_4:34132102-34132121 Msa0678420:CDS 40.0%
GAACAAGTTGTGTTGTATGC+CGG + chr4_4:34132159-34132178 Msa0678420:CDS 40.0%
AACATTTGCAAGGAATGCAG+TGG + chr4_4:34132220-34132239 Msa0678420:CDS 40.0%
CGGTTGGGTTATAGTTGTGT+TGG - chr4_4:34128890-34128909 None:intergenic 45.0%
GGAAAAGGGTTGTGGTCAAT+TGG - chr4_4:34128932-34128951 None:intergenic 45.0%
! TTGACCACAACCCTTTTCCT+TGG + chr4_4:34128933-34128952 Msa0678420:five_prime_UTR 45.0%
CGGTAGATTCGTCGAAATCA+TGG + chr4_4:34129218-34129237 Msa0678420:intron 45.0%
GCACCTAAATAGCAGTGAAG+TGG + chr4_4:34129734-34129753 Msa0678420:intron 45.0%
! GGAAAATCTAGTGGTGGAAG+TGG + chr4_4:34130368-34130387 Msa0678420:CDS 45.0%
CTGTCATTCGAACAACTCGA+GGG - chr4_4:34130743-34130762 None:intergenic 45.0%
ACTGTCATTCGAACAACTCG+AGG - chr4_4:34130744-34130763 None:intergenic 45.0%
CGAATGACAGTTCAGGGATT+AGG + chr4_4:34130753-34130772 Msa0678420:CDS 45.0%
CGAATTTCACTCGTTCGACA+TGG + chr4_4:34130813-34130832 Msa0678420:CDS 45.0%
ATTTCACTCGTTCGACATGG+TGG + chr4_4:34130816-34130835 Msa0678420:CDS 45.0%
!!! CTTGTTTTTGACTCCCCAGA+TGG + chr4_4:34130850-34130869 Msa0678420:CDS 45.0%
TCCCCAGATGGTTATCATGT+TGG + chr4_4:34130862-34130881 Msa0678420:CDS 45.0%
TTGCTAGTTCCATCTCCCTA+TGG + chr4_4:34131163-34131182 Msa0678420:CDS 45.0%
AGCAACTTTCCTCATCCGAA+CGG - chr4_4:34131269-34131288 None:intergenic 45.0%
AGCTCTTCCAGATTCTGCTA+GGG + chr4_4:34131366-34131385 Msa0678420:CDS 45.0%
CAAGAATCCCTAGCAGAATC+TGG - chr4_4:34131376-34131395 None:intergenic 45.0%
CGAGATACATGTCCATTGCA+TGG - chr4_4:34131411-34131430 None:intergenic 45.0%
TGCAATGGACATGTATCTCG+AGG + chr4_4:34131411-34131430 Msa0678420:CDS 45.0%
CTCAACTCTCACAAGACGAA+CGG + chr4_4:34131905-34131924 Msa0678420:CDS 45.0%
ATTTGCAAGGAATGCAGTGG+AGG + chr4_4:34132223-34132242 Msa0678420:CDS 45.0%
TTTGCAAGGAATGCAGTGGA+GGG + chr4_4:34132224-34132243 Msa0678420:CDS 45.0%
GAGACAGAAGTAGTGCGGTT+GGG - chr4_4:34128905-34128924 None:intergenic 50.0%
AGAGACAGAAGTAGTGCGGT+TGG - chr4_4:34128906-34128925 None:intergenic 50.0%
GTGCAGAGACAGAAGTAGTG+CGG - chr4_4:34128910-34128929 None:intergenic 50.0%
! GCCCCACTTCACTGCTATTT+AGG - chr4_4:34129740-34129759 None:intergenic 50.0%
CTGCACCTCCTGGAAAATCA+TGG - chr4_4:34130412-34130431 None:intergenic 50.0%
!! GATTTTCCAGGAGGTGCAGA+AGG + chr4_4:34130413-34130432 Msa0678420:CDS 50.0%
TCAAAACCTTCTGCACCTCC+TGG - chr4_4:34130422-34130441 None:intergenic 50.0%
! GCGTTGTGCTGCTGAATACA+TGG + chr4_4:34130508-34130527 Msa0678420:CDS 50.0%
GTTTGGATGCTGTAGTTGGG+AGG + chr4_4:34130687-34130706 Msa0678420:CDS 50.0%
!! TGTAGTTGGGAGGCTGGTTT+CGG + chr4_4:34130697-34130716 Msa0678420:CDS 50.0%
TTCGAACAACTCGAGGGACA+CGG - chr4_4:34130737-34130756 None:intergenic 50.0%
CAGCTCTTCCAGATTCTGCT+AGG + chr4_4:34131365-34131384 Msa0678420:CDS 50.0%
CGTTCGTCTTGTGAGAGTTG+AGG - chr4_4:34131907-34131926 None:intergenic 50.0%
! GAAAGGTGTAGGCATGCTTG+AGG - chr4_4:34131970-34131989 None:intergenic 50.0%
AGGAATGCAGTGGAGGGTTA+TGG + chr4_4:34132230-34132249 Msa0678420:CDS 50.0%
CGCGCGAGTAACAGATTCGA+AGG - chr4_4:34130491-34130510 None:intergenic 55.0%
!! TTGAAAGCGTTCCTGCGGCA+AGG + chr4_4:34131226-34131245 Msa0678420:CDS 55.0%
GGATGCTGTAGTTGGGAGGC+TGG + chr4_4:34130691-34130710 Msa0678420:CDS 60.0%
CTGGTGCGGTTGCTTCCGTT+CGG + chr4_4:34131251-34131270 Msa0678420:CDS 60.0%
CGGTTGCTTCCGTTCGGATG+AGG + chr4_4:34131257-34131276 Msa0678420:CDS 60.0%
GCGTTCCTGCGGCAAGGAAC+TGG + chr4_4:34131232-34131251 Msa0678420:CDS 65.0%
CCTGCGGCAAGGAACTGGTG+CGG + chr4_4:34131237-34131256 Msa0678420:CDS 65.0%
CCGCACCAGTTCCTTGCCGC+AGG - chr4_4:34131240-34131259 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 34128801 34132524 34128801 ID=Msa0678420;Name=Msa0678420
chr4_4 mRNA 34128801 34132524 34128801 ID=Msa0678420-mRNA-1;Parent=Msa0678420;Name=Msa0678420-mRNA-1;_AED=0.15;_eAED=0.15;_QI=319|1|1|1|1|1|3|165|544
chr4_4 exon 34128801 34129185 34128801 ID=Msa0678420-mRNA-1:exon:8213;Parent=Msa0678420-mRNA-1
chr4_4 exon 34130326 34131432 34130326 ID=Msa0678420-mRNA-1:exon:8214;Parent=Msa0678420-mRNA-1
chr4_4 exon 34131898 34132524 34131898 ID=Msa0678420-mRNA-1:exon:8215;Parent=Msa0678420-mRNA-1
chr4_4 five_prime_UTR 34128801 34129119 34128801 ID=Msa0678420-mRNA-1:five_prime_utr;Parent=Msa0678420-mRNA-1
chr4_4 CDS 34129120 34129185 34129120 ID=Msa0678420-mRNA-1:cds;Parent=Msa0678420-mRNA-1
chr4_4 CDS 34130326 34131432 34130326 ID=Msa0678420-mRNA-1:cds;Parent=Msa0678420-mRNA-1
chr4_4 CDS 34131898 34132359 34131898 ID=Msa0678420-mRNA-1:cds;Parent=Msa0678420-mRNA-1
chr4_4 three_prime_UTR 34132360 34132524 34132360 ID=Msa0678420-mRNA-1:three_prime_utr;Parent=Msa0678420-mRNA-1
Gene Sequence

>Msa0678420

ATGGATGAAGGTTTCTGCAATCTTGAAGTGGATGTAAATGGAGAAGAAACTTTCAAGGTGGATAAGACGATTATTACTCAATACTCGAGCAAATTGGCTAAAGTATTTGGAAAATCTAGTGGTGGAAGTGGAAAGCTTAAAGTAATATTCCATGATTTTCCAGGAGGTGCAGAAGGTTTTGAGCTTATGTTAAGATTCTGTTATAACAATGGAAAAGGTAATTTAAGTCCTTCGAATCTGTTACTCGCGCGTTGTGCTGCTGAATACATGGAAATGAATGAATCTGTCGAAAGTGTTTCTAACTTACTGGATCAAACTGAAAAATCGGTTCAAGGGATTAGTTATTGGTCATGGTCAGAAATTTTGATTGCTTTGAAGCAATGTCAGAATTTGCTAGTTGATGATTCTTCTCTGATGCTAGAGAAGTGTTTGGATGCTGTAGTTGGGAGGCTGGTTTCGGCGAGTGAAGCAAGTCCGTGTCCCTCGAGTTGTTCGAATGACAGTTCAGGGATTAGGTTTTCGTGTGACTCGAAAAGTACTGAGAGTATAAAGACGAATTTCACTCGTTCGACATGGTGGTTTGAAGATCTCTTGTTTTTGACTCCCCAGATGGTTATCATGTTGGTAAAGTCAATGCTTTCGAGTAAATTGGATCATTATGTTATAAGTAGGTTTCTTCTGTATTATCAGAAGGCGAAATTCGCTGCTGCTAATACAGATGAGAAGTGCAAAATCATTGAAATGGTCATAGATATGCATTACGAAATAGACCGGAGTTCTGTTTCTTTCAAAACATTATTTGGGATTCTTCGGGTGACTTTGTGTTTGAACATAAGTAAATGTAGCAGGAATAAGTTGGAGATTATGATCGGTTCACAATTGGATCAAGTAACATTGGACAATTTGCTAGTTCCATCTCCCTATGGAATAAACTACTTATATGATGTGAACCTTGTTCTGAGATTTTTGAAAGCGTTCCTGCGGCAAGGAACTGGTGCGGTTGCTTCCGTTCGGATGAGGAAAGTTGCTAGCTTAATAGATTTGTATATAGCTGAAATTGCACCTGATCCTTGTTTAAAAACTTCAAAGTTTTTGGCTCTTGCTACAGCTCTTCCAGATTCTGCTAGGGATTCTTGTGATGAGCTTTACCATGCAATGGACATGTATCTCGAGGTACATCCTCAACTCTCACAAGACGAACGGATAAAGATATGTTGTGGTTTAAATTACGAGAAACTTTCACCTCAAGCATGCCTACACCTTTCAAAGAACACGAAATTCCCATCAAAATCAGCAGTTCAAGCTCTCATATCTCAACAATCTAAGCTAAAAAATTTACTTCTCACTACTACTACTCCCATTTCAACTCCATACAATGATTCTTTGTGTGGTTCAAGTGGTTCTTCTCAAAAGGGAAAGAAAGAAATAACCAATGAACAAGTTGTGTTGTATGCCGGAAATTTCGATATTCAACCTGATAATGAGAAAATGAAAGAACATTTGCAAGGAATGCAGTGGAGGGTTATGGAATTAGAGAAAGTATGTAAGAAAATGCAAACTCAGATTGTTAAGATGACAAAATCAAAAGCATCTGGTCATAGTTATGGTAAATCTTTGCCAAAGCTATGTTCATGA
Protein sequence

>Msa0678420

MDEGFCNLEVDVNGEETFKVDKTIITQYSSKLAKVFGKSSGGSGKLKVIFHDFPGGAEGFELMLRFCYNNGKGNLSPSNLLLARCAAEYMEMNESVESVSNLLDQTEKSVQGISYWSWSEILIALKQCQNLLVDDSSLMLEKCLDAVVGRLVSASEASPCPSSCSNDSSGIRFSCDSKSTESIKTNFTRSTWWFEDLLFLTPQMVIMLVKSMLSSKLDHYVISRFLLYYQKAKFAAANTDEKCKIIEMVIDMHYEIDRSSVSFKTLFGILRVTLCLNISKCSRNKLEIMIGSQLDQVTLDNLLVPSPYGINYLYDVNLVLRFLKAFLRQGTGAVASVRMRKVASLIDLYIAEIAPDPCLKTSKFLALATALPDSARDSCDELYHAMDMYLEVHPQLSQDERIKICCGLNYEKLSPQACLHLSKNTKFPSKSAVQALISQQSKLKNLLLTTTTPISTPYNDSLCGSSGSSQKGKKEITNEQVVLYAGNFDIQPDNEKMKEHLQGMQWRVMELEKVCKKMQTQIVKMTKSKASGHSYGKSLPKLCS*