AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0640130

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0640130 MtrunA17_Chr4g0023801 96.881 545 16 1 1 544 1 545 0.0 1088
Msa0640130 MtrunA17_Chr4g0037661 34.331 568 319 13 6 543 5 548 3.80e-96 303
Msa0640130 MtrunA17_Chr2g0280541 37.727 440 240 8 75 505 3 417 1.72e-95 298
Msa0640130 MtrunA17_Chr1g0163311 34.838 554 296 14 5 526 4 524 1.34e-93 295
Msa0640130 MtrunA17_Chr8g0374011 32.000 550 311 16 48 541 82 624 2.12e-64 222
Msa0640130 MtrunA17_Chr2g0303541 30.823 571 322 13 14 534 35 582 1.81e-63 219
Msa0640130 MtrunA17_Chr2g0320691 28.021 571 349 16 7 531 26 580 2.30e-58 205
Msa0640130 MtrunA17_Chr2g0285511 27.931 580 321 19 14 537 35 573 1.27e-55 197
Msa0640130 MtrunA17_Chr7g0245201 29.856 556 315 17 5 513 49 576 2.10e-55 197
Msa0640130 MtrunA17_Chr1g0199631 30.090 555 315 17 29 539 14 539 3.02e-54 192
Msa0640130 MtrunA17_Chr4g0010091 28.623 559 344 15 17 542 46 582 1.15e-52 189
Msa0640130 MtrunA17_Chr1g0181181 27.289 557 304 18 14 521 35 539 1.10e-51 185
Msa0640130 MtrunA17_Chr5g0401451 28.445 566 350 19 11 542 43 587 1.80e-51 186
Msa0640130 MtrunA17_Chr2g0279481 27.213 610 345 16 14 542 53 644 2.03e-51 187
Msa0640130 MtrunA17_Chr4g0050021 27.010 622 340 18 7 543 47 639 5.79e-50 182
Msa0640130 MtrunA17_Chr1g0195911 27.069 580 351 15 7 542 39 590 1.93e-48 177
Msa0640130 MtrunA17_Chr4g0053841 27.221 529 333 15 14 513 22 527 4.90e-45 167
Msa0640130 MtrunA17_Chr7g0268781 26.056 568 362 13 14 542 45 593 5.41e-45 168
Msa0640130 MtrunA17_Chr5g0412591 28.665 457 227 15 48 442 70 489 6.79e-45 167
Msa0640130 MtrunA17_Chr4g0076521 30.162 431 258 14 40 442 60 475 1.07e-42 161
Msa0640130 MtrunA17_Chr1g0207461 27.716 451 290 11 52 472 74 518 1.30e-42 161
Msa0640130 MtrunA17_Chr8g0382231 26.067 445 251 11 50 444 97 513 7.68e-42 159
Msa0640130 MtrunA17_Chr5g0401341 27.527 465 284 12 14 442 36 483 4.42e-40 154
Msa0640130 MtrunA17_Chr3g0108331 25.813 523 310 15 14 505 33 508 1.28e-39 152
Msa0640130 MtrunA17_Chr1g0150191 26.477 457 300 10 14 440 36 486 1.02e-37 147
Msa0640130 MtrunA17_Chr1g0191711 26.185 443 281 11 14 440 67 479 1.35e-36 143
Msa0640130 MtrunA17_Chr8g0367891 26.622 447 256 13 43 442 63 484 5.19e-35 139
Msa0640130 MtrunA17_Chr2g0290741 27.857 420 252 14 50 442 70 465 7.60e-34 135
Msa0640130 MtrunA17_Chr5g0432861 24.841 471 284 10 14 445 34 473 1.46e-33 134
Msa0640130 MtrunA17_Chr2g0332281 32.971 276 161 6 257 527 106 362 2.10e-33 132
Msa0640130 MtrunA17_Chr4g0003891 30.526 285 187 4 257 539 126 401 5.73e-32 128
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0640130 AT3G22104.1 47.263 548 243 7 1 544 1 506 2.04e-159 464
Msa0640130 AT3G22104.4 45.975 472 209 7 77 544 2 431 6.70e-126 375
Msa0640130 AT3G22104.2 45.975 472 209 7 77 544 2 431 6.70e-126 375
Msa0640130 AT3G22104.3 45.975 472 209 7 77 544 2 431 6.70e-126 375
Msa0640130 AT3G19850.1 34.452 566 327 15 6 543 5 554 2.17e-94 298
Msa0640130 AT1G50280.1 34.475 467 263 12 7 439 6 463 9.11e-73 241
Msa0640130 AT1G50280.2 35.561 419 231 11 51 439 21 430 3.97e-67 225
Msa0640130 AT5G66560.3 32.772 534 308 12 43 531 71 598 1.69e-66 227
Msa0640130 AT5G66560.4 32.772 534 308 12 43 531 61 588 2.07e-66 227
Msa0640130 AT5G66560.1 32.772 534 308 12 43 531 100 627 3.68e-66 227
Msa0640130 AT3G08570.1 28.822 569 324 16 7 533 37 566 1.97e-59 208
Msa0640130 AT3G50840.1 28.933 553 336 13 7 537 19 536 1.86e-57 201
Msa0640130 AT3G50840.2 29.804 510 302 12 50 537 25 500 2.73e-56 197
Msa0640130 AT3G50840.3 29.804 510 302 12 50 537 25 500 2.73e-56 197
Msa0640130 AT1G30440.1 29.668 573 340 14 14 534 35 596 2.85e-55 197
Msa0640130 AT5G03250.1 27.749 573 333 15 14 539 35 573 1.56e-54 194
Msa0640130 AT5G13600.1 29.101 567 319 19 15 537 36 563 1.75e-54 194
Msa0640130 AT5G48800.1 29.623 557 350 17 7 542 44 579 3.97e-54 193
Msa0640130 AT3G08660.1 28.417 556 328 15 10 541 40 549 1.12e-53 191
Msa0640130 AT3G08660.3 28.682 516 301 13 50 541 17 489 2.73e-52 186
Msa0640130 AT3G44820.1 25.714 560 351 11 18 524 36 583 2.61e-50 183
Msa0640130 AT1G03010.2 28.693 589 344 19 7 544 12 575 3.51e-50 182
Msa0640130 AT1G03010.3 28.693 589 344 19 7 544 38 601 3.60e-50 182
Msa0640130 AT1G03010.1 28.693 589 344 19 7 544 39 602 5.22e-50 182
Msa0640130 AT5G66560.2 35.401 387 207 11 43 392 100 480 2.42e-49 177
Msa0640130 AT5G67385.1 29.605 456 283 12 14 444 34 476 2.88e-48 177
Msa0640130 AT2G47860.1 28.092 566 326 18 7 524 40 572 8.18e-48 176
Msa0640130 AT5G67385.4 29.909 438 269 12 32 444 7 431 1.70e-47 174
Msa0640130 AT5G67385.5 29.909 438 269 12 32 444 7 431 1.70e-47 174
Msa0640130 AT5G67385.2 29.909 438 269 12 32 444 7 431 1.70e-47 174
Msa0640130 AT5G67385.3 29.909 438 269 12 32 444 7 431 1.70e-47 174
Msa0640130 AT2G47860.3 28.433 517 298 15 54 524 105 595 6.72e-46 171
Msa0640130 AT5G47800.3 28.704 432 254 13 43 440 63 474 6.73e-44 164
Msa0640130 AT5G47800.1 28.704 432 254 13 43 440 63 474 6.73e-44 164
Msa0640130 AT5G47800.4 28.704 432 254 13 43 440 63 474 6.73e-44 164
Msa0640130 AT5G47800.2 28.704 432 254 13 43 440 176 587 3.26e-43 164
Msa0640130 AT2G23050.1 28.141 398 250 9 52 440 78 448 3.52e-42 158
Msa0640130 AT3G49970.1 28.103 427 253 11 17 427 6 394 1.01e-41 157
Msa0640130 AT3G49970.2 28.103 427 253 11 17 427 6 394 1.01e-41 157
Msa0640130 AT2G14820.2 27.815 453 284 12 53 470 74 518 1.05e-41 159
Msa0640130 AT2G14820.1 27.815 453 284 12 53 470 74 518 1.05e-41 159
Msa0640130 AT5G03250.2 26.749 486 278 12 100 539 8 461 1.98e-41 155
Msa0640130 AT3G26490.1 29.565 460 254 15 52 456 75 519 2.61e-40 154
Msa0640130 AT3G26490.3 29.565 460 254 15 52 456 75 519 2.61e-40 154
Msa0640130 AT3G26490.4 29.565 460 254 15 52 456 75 519 2.61e-40 154
Msa0640130 AT2G30520.1 27.802 464 273 10 14 445 39 472 6.50e-39 150
Msa0640130 AT1G67900.5 26.829 451 260 11 52 442 69 509 7.19e-38 148
Msa0640130 AT1G67900.1 26.829 451 260 11 52 442 69 509 7.19e-38 148
Msa0640130 AT1G67900.3 26.829 451 260 11 52 442 69 509 7.19e-38 148
Msa0640130 AT1G67900.6 26.829 451 260 11 52 442 69 509 7.19e-38 148
Msa0640130 AT1G67900.2 26.829 451 260 11 52 442 69 509 7.19e-38 148
Msa0640130 AT1G67900.4 26.829 451 260 11 52 442 69 509 7.19e-38 148
Msa0640130 AT2G30520.2 28.082 438 254 9 40 445 16 424 1.27e-37 146
Msa0640130 AT2G30520.3 28.082 438 254 9 40 445 16 424 1.27e-37 146
Msa0640130 AT3G08660.2 29.032 403 242 11 10 393 40 417 4.71e-37 142
Msa0640130 AT5G67440.3 25.510 490 318 13 10 475 32 498 6.41e-36 142
Msa0640130 AT5G67440.1 25.510 490 318 13 10 475 32 498 6.41e-36 142
Msa0640130 AT5G67440.2 25.690 471 303 13 29 475 14 461 3.73e-35 139
Msa0640130 AT2G47860.5 27.021 470 273 13 101 524 9 454 4.14e-34 135
Msa0640130 AT2G47860.2 27.021 470 273 13 101 524 9 454 4.14e-34 135
Msa0640130 AT4G37590.1 26.809 470 292 13 14 457 36 479 1.09e-33 135
Msa0640130 AT2G47860.4 28.605 430 238 14 7 392 40 444 1.39e-33 133
Msa0640130 AT4G31820.1 26.190 462 292 15 7 440 26 466 1.40e-33 135
Msa0640130 AT4G31820.2 26.047 430 273 13 35 440 5 413 4.97e-32 129
Msa0640130 AT2G47860.6 29.504 383 206 12 54 392 105 467 6.30e-32 129
Msa0640130 AT3G26490.2 29.293 396 212 14 52 392 75 457 1.91e-31 127
Msa0640130 AT1G52770.1 31.507 292 176 6 257 539 116 392 2.70e-31 126
Msa0640130 AT5G64330.1 31.804 327 177 5 258 542 388 710 1.17e-30 127
Msa0640130 AT5G64330.1 23.770 244 142 7 14 229 61 288 1.45e-13 73.9
Msa0640130 AT2G23050.2 26.816 358 227 8 92 440 1 332 1.33e-30 123
Msa0640130 AT3G15570.1 30.000 290 175 4 257 536 121 392 5.69e-30 122
Msa0640130 AT5G64330.2 34.711 242 117 4 258 462 388 625 1.01e-28 121
Msa0640130 AT5G64330.2 23.770 244 142 7 14 229 61 288 8.73e-14 74.7
Msa0640130 AT5G64330.3 34.118 255 126 5 245 462 317 566 1.25e-28 120
Msa0640130 AT5G64330.3 24.762 210 115 6 48 229 35 229 3.90e-13 72.4
Msa0640130 AT5G48130.1 26.225 408 267 12 49 440 78 467 4.11e-27 116
Msa0640130 AT5G10250.2 27.740 292 174 6 260 536 311 580 9.29e-27 115
Msa0640130 AT5G10250.2 32.800 125 81 3 7 131 53 174 2.28e-11 67.0
Msa0640130 AT5G10250.1 27.758 281 173 5 260 536 311 565 4.69e-25 109
Msa0640130 AT5G10250.1 32.800 125 81 3 7 131 53 174 2.55e-11 66.6
Msa0640130 AT3G03510.1 25.316 395 248 14 60 445 8 364 4.85e-19 90.5
Msa0640130 AT5G17580.1 23.516 455 315 15 7 445 8 445 3.59e-16 82.0
Msa0640130 AT5G17580.2 23.516 455 315 15 7 445 23 460 3.72e-16 82.0

Find 118 sgRNAs with CRISPR-Local

Find 205 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTTCGTTCAAAACATTATT+TGG 0.105477 4_3:+37795406 Msa0640130:CDS
AGCAAATTGGCTAAAGTATT+TGG 0.117951 4_3:+37794713 Msa0640130:CDS
GTCAATGCTTTCGAGTAAAT+TGG 0.197083 4_3:+37795255 Msa0640130:CDS
TGTGTGTTGTACTGCTTAAT+TGG 0.279730 4_3:+37796801 Msa0640130:three_prime_UTR
CGAACGACAGTTCAGGGATT+AGG 0.296707 4_3:+37795119 Msa0640130:CDS
ATCATTGTATGGAGTTGAAA+TGG 0.302995 4_3:-37796449 None:intergenic
AAACATTATTTGGGATTCTT+CGG 0.325788 4_3:+37795416 Msa0640130:CDS
AATCATCAACTAGCAAATTC+TGG 0.336537 4_3:-37795009 None:intergenic
TACTCAATACTCGAGCAAAT+TGG 0.347083 4_3:+37794700 Msa0640130:CDS
AATCTTGAAGTGGATGTAAA+TGG 0.347819 4_3:+37793507 Msa0640130:CDS
AACATTATTTGGGATTCTTC+GGG 0.354248 4_3:+37795417 Msa0640130:CDS
AGTTGTTCGAACGACAGTTC+AGG 0.359093 4_3:+37795112 Msa0640130:CDS
TTTCGTTCAAAACATTATTT+GGG 0.365679 4_3:+37795407 Msa0640130:CDS
AAAGAATCCCTAGCAGAATC+TGG 0.366057 4_3:-37795739 None:intergenic
ATACAACACAACTTGTTCAT+TGG 0.366068 4_3:-37796521 None:intergenic
ATTGTACAGTCACTAAATAA+TGG 0.370741 4_3:+37796853 Msa0640130:three_prime_UTR
GATTATGATTGGTTCACAAT+TGG 0.371651 4_3:+37795486 Msa0640130:CDS
TGTAGTTGGGAGGCTGGTTT+CGG 0.375981 4_3:+37795063 Msa0640130:CDS
TTCAAGTGGTTCTTCTCAAA+AGG 0.390409 4_3:+37796483 Msa0640130:CDS
CGGTTCAAGGGATTAGCTAT+TGG 0.391036 4_3:+37794951 Msa0640130:CDS
AATAAGTTGGAGATTATGAT+TGG 0.392496 4_3:+37795475 Msa0640130:CDS
CTGCTTAATTGGTGTTGGTT+GGG 0.396179 4_3:+37796812 Msa0640130:three_prime_UTR
GAAAGACAAAACCAAGGAAA+AGG 0.412154 4_3:-37793313 None:intergenic
GGATGCTGTAGTTGGGAGGC+TGG 0.417675 4_3:+37795057 Msa0640130:CDS
AAATGGGAGTAGTAGTAGTA+AGG 0.420418 4_3:-37796432 None:intergenic
CTGTCGTTCGAACAACTTGA+TGG 0.420772 4_3:-37795106 None:intergenic
GCGTTCCTGCGGCAAGGAAC+TGG 0.427410 4_3:+37795598 Msa0640130:CDS
TTTCTAGTTATTATGGAAGA+AGG 0.429606 4_3:+37793477 Msa0640130:exon
AGAAACTTTCATGGTGGATA+AGG 0.429758 4_3:+37793533 Msa0640130:CDS
TGGATCATTATGTTATAAGT+AGG 0.433791 4_3:+37795275 Msa0640130:CDS
GCAACTTTCCGCATCTGAAC+GGG 0.435207 4_3:-37795631 None:intergenic
AATGGGGATGGAAGAGGAAA+AGG 0.439990 4_3:-37793405 None:intergenic
TGAACATAAGTAAATGTAGT+AGG 0.441739 4_3:+37795452 Msa0640130:CDS
GAAGTGCAAAATCATTGAAA+TGG 0.446426 4_3:+37795348 Msa0640130:CDS
AGGAATGCAATGGAGGGTTA+TGG 0.450787 4_3:+37796597 Msa0640130:CDS
AAAGCATCTGGTCATAGTTA+TGG 0.455369 4_3:+37796676 Msa0640130:CDS
GGAAAAGGGTTGTGGTCAAT+TGG 0.456525 4_3:-37793298 None:intergenic
TTACCAACATGATAACCATC+TGG 0.456686 4_3:-37795231 None:intergenic
TTGCTTGTTCCATCTCCCTA+TGG 0.458028 4_3:+37795529 Msa0640130:CDS
TGTGATGCTAGAGAAGTGTT+TGG 0.459519 4_3:+37795036 Msa0640130:CDS
CCACACAAAGAATCATTGTA+TGG 0.462883 4_3:-37796460 None:intergenic
ATGACAAAATCAAAAGCATC+TGG 0.463126 4_3:+37796664 Msa0640130:CDS
GGGTTATAGTTGTGTTGGAA+TGG 0.469151 4_3:-37793251 None:intergenic
ACTAGTCATGAACATAGCTT+TGG 0.471640 4_3:-37796709 None:intergenic
TCATTGTATGGAGTTGAAAT+GGG 0.472028 4_3:-37796448 None:intergenic
ACTGCTTAATTGGTGTTGGT+TGG 0.473548 4_3:+37796811 Msa0640130:three_prime_UTR
TTCTGTTATAACAATGGAAA+AGG 0.474857 4_3:+37794821 Msa0640130:CDS
TCAAAACCTTCTGCACCTCC+TGG 0.479630 4_3:-37794785 None:intergenic
ATATAAGTAGTTTATTCCAT+AGG 0.480644 4_3:-37795545 None:intergenic
TTGTACTGCTTAATTGGTGT+TGG 0.480697 4_3:+37796807 Msa0640130:three_prime_UTR
AGTGTTTGGATGCTGTAGTT+GGG 0.482062 4_3:+37795050 Msa0640130:CDS
TAAATGTAGTAGGAATAAGT+TGG 0.484168 4_3:+37795462 Msa0640130:CDS
AAGTGTTTGGATGCTGTAGT+TGG 0.484528 4_3:+37795049 Msa0640130:CDS
GTTGTTCGAACGACAGTTCA+GGG 0.487608 4_3:+37795113 Msa0640130:CDS
CAAACTGAAAAGTCGGTTCA+AGG 0.493445 4_3:+37794938 Msa0640130:CDS
TATAAGTAGTTTATTCCATA+GGG 0.496705 4_3:-37795544 None:intergenic
AAATGGAGAAGAAACTTTCA+TGG 0.499209 4_3:+37793524 Msa0640130:CDS
GGAAAATCTAGTGGTGGAAG+TGG 0.507767 4_3:+37794734 Msa0640130:CDS
CTGCACCTCCTGGAAAATCA+TGG 0.509317 4_3:-37794775 None:intergenic
TCAAGTGGTTCTTCTCAAAA+GGG 0.512620 4_3:+37796484 Msa0640130:CDS
AAAGACAAAACCAAGGAAAA+GGG 0.518371 4_3:-37793312 None:intergenic
CAGCTCTTCCAGATTCTGCT+AGG 0.520217 4_3:+37795731 Msa0640130:CDS
GAACAAGTTGTGTTGTATGC+TGG 0.522427 4_3:+37796526 Msa0640130:CDS
CCATACAATGATTCTTTGTG+TGG 0.523116 4_3:+37796460 Msa0640130:CDS
GCTTGAACCGCTGATTTCGA+TGG 0.531417 4_3:-37796373 None:intergenic
GAGACAGAAGTAGTGCGGTT+GGG 0.532780 4_3:-37793271 None:intergenic
TACCAACATGATAACCATCT+GGG 0.536405 4_3:-37795230 None:intergenic
CGGTTGGGTTATAGTTGTGT+TGG 0.537400 4_3:-37793256 None:intergenic
ACAATTGGATCAAGTAACAT+TGG 0.540829 4_3:+37795501 Msa0640130:CDS
GTTTCTTCTGTATTATCAGA+AGG 0.541194 4_3:+37795297 Msa0640130:CDS
CCGCACCAGTTCCTTGCCGC+AGG 0.541269 4_3:-37795603 None:intergenic
TCCCCAGATGGTTATCATGT+TGG 0.541685 4_3:+37795228 Msa0640130:CDS
CGAATTTCACTCGTTCGACA+TGG 0.541979 4_3:+37795179 Msa0640130:CDS
TAGTTTATTCCATAGGGAGA+TGG 0.542308 4_3:-37795538 None:intergenic
TTAAGATTCTGTTATAACAA+TGG 0.544300 4_3:+37794815 Msa0640130:CDS
AGCAACTTTCCGCATCTGAA+CGG 0.556641 4_3:-37795632 None:intergenic
AGAGACAGAAGTAGTGCGGT+TGG 0.558217 4_3:-37793272 None:intergenic
ACATGAAGTGATATTCTAAG+AGG 0.561631 4_3:-37793363 None:intergenic
CGTTCGTCTTGTGAGAGTTG+AGG 0.564013 4_3:-37796271 None:intergenic
AAAACCAAGGAAAAGGGTTG+TGG 0.564062 4_3:-37793306 None:intergenic
AGCTCTTCCAGATTCTGCTA+GGG 0.564119 4_3:+37795732 Msa0640130:CDS
ATCGGATCAAACTGAAAAGT+CGG 0.564539 4_3:+37794931 Msa0640130:CDS
ATTTGCAAGGAATGCAATGG+AGG 0.565698 4_3:+37796590 Msa0640130:CDS
TGATGAACTCTACCATGCAA+TGG 0.566425 4_3:+37795762 Msa0640130:CDS
TTGAAAGCGTTCCTGCGGCA+AGG 0.567464 4_3:+37795592 Msa0640130:CDS
GATTCTTTGTGTGGTTCAAG+TGG 0.575011 4_3:+37796469 Msa0640130:CDS
CGCGCGAGTAACAGATTCGA+AGG 0.577244 4_3:-37794854 None:intergenic
AACATTTGCAAGGAATGCAA+TGG 0.581610 4_3:+37796587 Msa0640130:CDS
AAGGGATTAGCTATTGGTCA+TGG 0.581611 4_3:+37794957 Msa0640130:CDS
AAACTGAAAAGTCGGTTCAA+GGG 0.583502 4_3:+37794939 Msa0640130:CDS
ATATGCATTACGAAATAGAC+CGG 0.586731 4_3:+37795377 Msa0640130:CDS
AGGTTTCTGTAATCTTGAAG+TGG 0.588076 4_3:+37793497 Msa0640130:CDS
AAAGTATTTGGAAAATCTAG+TGG 0.598023 4_3:+37794725 Msa0640130:CDS
GCGTTGTGCTGCTGAATACA+TGG 0.598711 4_3:+37794874 Msa0640130:CDS
CGGTTGCTCCCGTTCAGATG+CGG 0.601268 4_3:+37795623 Msa0640130:CDS
TTACATATAATATTGTGTGG+AGG 0.609904 4_3:-37793167 None:intergenic
CTCAACTCTCACAAGACGAA+CGG 0.610795 4_3:+37796272 Msa0640130:CDS
CGAGATACATGTCCATTGCA+TGG 0.615686 4_3:-37795774 None:intergenic
AAAATGAAAGAACATTTGCA+AGG 0.616420 4_3:+37796577 Msa0640130:CDS
GTTTGGATGCTGTAGTTGGG+AGG 0.620714 4_3:+37795053 Msa0640130:CDS
ATTTCACTCGTTCGACATGG+TGG 0.628952 4_3:+37795182 Msa0640130:CDS
GATTAGCTATTGGTCATGGT+CGG 0.629784 4_3:+37794961 Msa0640130:CDS
TTTGCAAGGAATGCAATGGA+GGG 0.646170 4_3:+37796591 Msa0640130:CDS
GAAAGGTGTAGGCATGCTTG+AGG 0.646257 4_3:-37796334 None:intergenic
GTGTTACATATAATATTGTG+TGG 0.649183 4_3:-37793170 None:intergenic
TGCAATGGACATGTATCTCG+AGG 0.652835 4_3:+37795777 Msa0640130:CDS
CTTCTCATCTGTATTAGCAG+CGG 0.653745 4_3:-37795329 None:intergenic
GTATTTGGAAAATCTAGTGG+TGG 0.653932 4_3:+37794728 Msa0640130:CDS
AAGACAGAAAGACAAAACCA+AGG 0.657843 4_3:-37793319 None:intergenic
CCTGCGGCAAGGAACTGGTG+CGG 0.658037 4_3:+37795603 Msa0640130:CDS
GAACGGATAAAGATATGCTG+TGG 0.658530 4_3:+37796289 Msa0640130:CDS
AATGGATACAACAAGGACAA+AGG 0.667374 4_3:-37793233 None:intergenic
TGGAGAAGAAACTTTCATGG+TGG 0.676896 4_3:+37793527 Msa0640130:CDS
ACCAACATGATAACCATCTG+GGG 0.676918 4_3:-37795229 None:intergenic
TTCGAACAACTTGATGGACA+CGG 0.685263 4_3:-37795100 None:intergenic
GAAATTTCCATCGAAATCAG+CGG 0.691407 4_3:+37796366 Msa0640130:CDS
GTGCAGAGACAGAAGTAGTG+CGG 0.703525 4_3:-37793276 None:intergenic
GTGTTGGAATGGATACAACA+AGG 0.725933 4_3:-37793240 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTTTGTATGTTTTAAT+AGG + chr4_3:37793848-37793867 Msa0640130:intron 10.0%
!! TAAACTAAAATTGTTTAAAT+TGG + chr4_3:37794658-37794677 Msa0640130:intron 10.0%
!!! TCAAAATTTAAAATTCAAAA+TGG - chr4_3:37795817-37795836 None:intergenic 10.0%
!!! ATTTTTCTTTCTAGTTATTA+TGG + chr4_3:37793470-37793489 Msa0640130:exon 15.0%
!! TTAATATTATAGGATTTACA+AGG + chr4_3:37793893-37793912 Msa0640130:intron 15.0%
!! TTATGATTTATGATGATTAA+TGG + chr4_3:37793931-37793950 Msa0640130:intron 15.0%
!!! CTTTAATTTAGAAATGATTA+TGG + chr4_3:37793981-37794000 Msa0640130:intron 15.0%
!! GTGAAAATAAAAAATTACTA+GGG - chr4_3:37794020-37794039 None:intergenic 15.0%
!! AGTGAAAATAAAAAATTACT+AGG - chr4_3:37794021-37794040 None:intergenic 15.0%
!!! TTTTATTAACATTTTTTGAC+AGG + chr4_3:37794309-37794328 Msa0640130:intron 15.0%
!! AAAAAATAATAAATCAAGCA+TGG - chr4_3:37794380-37794399 None:intergenic 15.0%
!! TATAAAGATAGGAAAATATA+TGG - chr4_3:37794521-37794540 None:intergenic 15.0%
!!! TTTAAAAACTTCAAAGTTTT+TGG + chr4_3:37795699-37795718 Msa0640130:CDS 15.0%
!!! ATAAAGATTTCTTACAATTT+TGG - chr4_3:37796042-37796061 None:intergenic 15.0%
!! GTACTAGTAGTTAATATTAT+AGG + chr4_3:37793883-37793902 Msa0640130:intron 20.0%
!! ATTTAGGTGCAAATTAATTA+TGG - chr4_3:37794088-37794107 None:intergenic 20.0%
!! TAAAAACAAACAAAATACGA+AGG - chr4_3:37794294-37794313 None:intergenic 20.0%
!!! TTTCTGTGCTTTAATTATTT+TGG + chr4_3:37794476-37794495 Msa0640130:intron 20.0%
!!! ATGTTTTTGGTAAGTTATTT+TGG + chr4_3:37794579-37794598 Msa0640130:intron 20.0%
!! TTAAGATTCTGTTATAACAA+TGG + chr4_3:37794815-37794834 Msa0640130:CDS 20.0%
!!! GAAAAATGTTTCTAACTTAT+CGG + chr4_3:37794913-37794932 Msa0640130:CDS 20.0%
!! TTTCGTTCAAAACATTATTT+GGG + chr4_3:37795407-37795426 Msa0640130:CDS 20.0%
!! TATAAGTAGTTTATTCCATA+GGG - chr4_3:37795547-37795566 None:intergenic 20.0%
!! ATATAAGTAGTTTATTCCAT+AGG - chr4_3:37795548-37795567 None:intergenic 20.0%
!!! AACTTTGAAGTTTTTAAACA+AGG - chr4_3:37795697-37795716 None:intergenic 20.0%
!!! AATTTTGAAGTCTATAACTT+TGG + chr4_3:37795829-37795848 Msa0640130:intron 20.0%
!! TAACATAGTAAATTTGAATG+CGG + chr4_3:37796005-37796024 Msa0640130:intron 20.0%
!!! TTTCTTACAATTTTGGATAA+GGG - chr4_3:37796035-37796054 None:intergenic 20.0%
!!! ATTTCTTACAATTTTGGATA+AGG - chr4_3:37796036-37796055 None:intergenic 20.0%
!! ATTAATAAAGATGACGTTAA+TGG - chr4_3:37796222-37796241 None:intergenic 20.0%
!!! TTTTCACATTTGTATAGTAA+AGG + chr4_3:37796767-37796786 Msa0640130:three_prime_UTR 20.0%
! GTGTTACATATAATATTGTG+TGG - chr4_3:37793173-37793192 None:intergenic 25.0%
! TCAGTTGAGAAAACAAAAAT+GGG - chr4_3:37793425-37793444 None:intergenic 25.0%
! TTCAGTTGAGAAAACAAAAA+TGG - chr4_3:37793426-37793445 None:intergenic 25.0%
!!! TTTCTAGTTATTATGGAAGA+AGG + chr4_3:37793477-37793496 Msa0640130:exon 25.0%
!!! TTTTTTGTAAAGTGTATGTC+TGG + chr4_3:37793559-37793578 Msa0640130:intron 25.0%
!!! AACCATTATGATTTTGAGTA+TGG + chr4_3:37793679-37793698 Msa0640130:intron 25.0%
! TTCCATACTCAAAATCATAA+TGG - chr4_3:37793684-37793703 None:intergenic 25.0%
!! GACTAAGTTTAGTTTATGTT+TGG - chr4_3:37793721-37793740 None:intergenic 25.0%
! TACAATCATAAAGTTACCAA+GGG - chr4_3:37794053-37794072 None:intergenic 25.0%
! GACAATAAATTGTAGAATGA+GGG - chr4_3:37794429-37794448 None:intergenic 25.0%
! AGACAATAAATTGTAGAATG+AGG - chr4_3:37794430-37794449 None:intergenic 25.0%
!!! CTCTAGTTATTTCATGTTTT+TGG + chr4_3:37794566-37794585 Msa0640130:intron 25.0%
!! AAAGTATTTGGAAAATCTAG+TGG + chr4_3:37794725-37794744 Msa0640130:CDS 25.0%
! TTCTGTTATAACAATGGAAA+AGG + chr4_3:37794821-37794840 Msa0640130:CDS 25.0%
! TGGATCATTATGTTATAAGT+AGG + chr4_3:37795275-37795294 Msa0640130:CDS 25.0%
! GTTTCGTTCAAAACATTATT+TGG + chr4_3:37795406-37795425 Msa0640130:CDS 25.0%
!! AAACATTATTTGGGATTCTT+CGG + chr4_3:37795416-37795435 Msa0640130:CDS 25.0%
! TGAACATAAGTAAATGTAGT+AGG + chr4_3:37795452-37795471 Msa0640130:CDS 25.0%
! TAAATGTAGTAGGAATAAGT+TGG + chr4_3:37795462-37795481 Msa0640130:CDS 25.0%
!! AATAAGTTGGAGATTATGAT+TGG + chr4_3:37795475-37795494 Msa0640130:CDS 25.0%
! CTTTCAAAAATCTCAAAACA+AGG - chr4_3:37795579-37795598 None:intergenic 25.0%
! TAATCAAGTAGTTAACTACA+TGG - chr4_3:37795855-37795874 None:intergenic 25.0%
! AATCTTTATCTTCTACTCAT+TGG + chr4_3:37796053-37796072 Msa0640130:intron 25.0%
! TAATCACATCTACATCAAAA+CGG + chr4_3:37796185-37796204 Msa0640130:intron 25.0%
! ATAATTTCCTCTTCTGTTAT+AGG + chr4_3:37796243-37796262 Msa0640130:intron 25.0%
!!! GAAATTTCGTGTTTTTTGAA+AGG - chr4_3:37796354-37796373 None:intergenic 25.0%
!! TCTTTCATTTTCTCATTATC+TGG - chr4_3:37796568-37796587 None:intergenic 25.0%
! AAAATGAAAGAACATTTGCA+AGG + chr4_3:37796577-37796596 Msa0640130:CDS 25.0%
! ATTGTACAGTCACTAAATAA+TGG + chr4_3:37796853-37796872 Msa0640130:three_prime_UTR 25.0%
AAAGACAAAACCAAGGAAAA+GGG - chr4_3:37793315-37793334 None:intergenic 30.0%
ACATGAAGTGATATTCTAAG+AGG - chr4_3:37793366-37793385 None:intergenic 30.0%
CAGTTGAGAAAACAAAAATG+GGG - chr4_3:37793424-37793443 None:intergenic 30.0%
AATCTTGAAGTGGATGTAAA+TGG + chr4_3:37793507-37793526 Msa0640130:CDS 30.0%
AAATGGAGAAGAAACTTTCA+TGG + chr4_3:37793524-37793543 Msa0640130:CDS 30.0%
! GATTTACAAGGTTTGTCTTT+TGG + chr4_3:37793905-37793924 Msa0640130:intron 30.0%
CTACAATCATAAAGTTACCA+AGG - chr4_3:37794054-37794073 None:intergenic 30.0%
AGGTGCAAATTAATTATGGA+TGG - chr4_3:37794084-37794103 None:intergenic 30.0%
!! AAAAAAGTGTCTTGTGTTTG+TGG - chr4_3:37794178-37794197 None:intergenic 30.0%
! ATGGTCTTCTAATGCTTTTA+TGG + chr4_3:37794264-37794283 Msa0640130:intron 30.0%
GAAACCAAAAACATTGAATG+AGG - chr4_3:37794460-37794479 None:intergenic 30.0%
AAGTAAGCAGGTATAAAGAT+AGG - chr4_3:37794532-37794551 None:intergenic 30.0%
! AGCAAATTGGCTAAAGTATT+TGG + chr4_3:37794713-37794732 Msa0640130:CDS 30.0%
! GTAATATTCCATGATTTTCC+AGG + chr4_3:37794767-37794786 Msa0640130:CDS 30.0%
AATCATCAACTAGCAAATTC+TGG - chr4_3:37795012-37795031 None:intergenic 30.0%
GTTTCTTCTGTATTATCAGA+AGG + chr4_3:37795297-37795316 Msa0640130:CDS 30.0%
GAAGTGCAAAATCATTGAAA+TGG + chr4_3:37795348-37795367 Msa0640130:CDS 30.0%
ATATGCATTACGAAATAGAC+CGG + chr4_3:37795377-37795396 Msa0640130:CDS 30.0%
! AACATTATTTGGGATTCTTC+GGG + chr4_3:37795417-37795436 Msa0640130:CDS 30.0%
! GATTATGATTGGTTCACAAT+TGG + chr4_3:37795486-37795505 Msa0640130:CDS 30.0%
ACAATTGGATCAAGTAACAT+TGG + chr4_3:37795501-37795520 Msa0640130:CDS 30.0%
! GAAGTTTTTAAACAAGGATC+AGG - chr4_3:37795691-37795710 None:intergenic 30.0%
!! ACAAAACTAATCGACTTGTA+AGG - chr4_3:37795955-37795974 None:intergenic 30.0%
!!! TACAAGTCGATTAGTTTTGT+AGG + chr4_3:37795955-37795974 Msa0640130:intron 30.0%
!!! ACAAGTCGATTAGTTTTGTA+GGG + chr4_3:37795956-37795975 Msa0640130:intron 30.0%
ACATCAAAACGGTTGTAATA+CGG + chr4_3:37796196-37796215 Msa0640130:intron 30.0%
TCATTGTATGGAGTTGAAAT+GGG - chr4_3:37796451-37796470 None:intergenic 30.0%
ATCATTGTATGGAGTTGAAA+TGG - chr4_3:37796452-37796471 None:intergenic 30.0%
! ATACAACACAACTTGTTCAT+TGG - chr4_3:37796524-37796543 None:intergenic 30.0%
!! ATGACAAAATCAAAAGCATC+TGG + chr4_3:37796664-37796683 Msa0640130:CDS 30.0%
AATGGATACAACAAGGACAA+AGG - chr4_3:37793236-37793255 None:intergenic 35.0%
GAAAGACAAAACCAAGGAAA+AGG - chr4_3:37793316-37793335 None:intergenic 35.0%
AAGACAGAAAGACAAAACCA+AGG - chr4_3:37793322-37793341 None:intergenic 35.0%
TGAGAAAACAAAAATGGGGA+TGG - chr4_3:37793420-37793439 None:intergenic 35.0%
! TGTTTTCTCAACTGAACAGA+AGG + chr4_3:37793430-37793449 Msa0640130:five_prime_UTR 35.0%
AGGTTTCTGTAATCTTGAAG+TGG + chr4_3:37793497-37793516 Msa0640130:CDS 35.0%
!! AGAAACTTTCATGGTGGATA+AGG + chr4_3:37793533-37793552 Msa0640130:CDS 35.0%
AAAGTGTATGTCTGGTATCA+CGG + chr4_3:37793567-37793586 Msa0640130:intron 35.0%
CAAGTGTATGTCTGATATCA+TGG + chr4_3:37793635-37793654 Msa0640130:intron 35.0%
AAGGAAAATCATTGCAAGCA+TGG - chr4_3:37794140-37794159 None:intergenic 35.0%
!!! ACATTTTTTGACAGGATCTG+AGG + chr4_3:37794317-37794336 Msa0640130:intron 35.0%
!!! CATTTTTTGACAGGATCTGA+GGG + chr4_3:37794318-37794337 Msa0640130:intron 35.0%
! ACGACCTCATTCAATGTTTT+TGG + chr4_3:37794453-37794472 Msa0640130:intron 35.0%
CATACACAAGAAAAGTAAGC+AGG - chr4_3:37794544-37794563 None:intergenic 35.0%
TACTCAATACTCGAGCAAAT+TGG + chr4_3:37794700-37794719 Msa0640130:CDS 35.0%
GTATTTGGAAAATCTAGTGG+TGG + chr4_3:37794728-37794747 Msa0640130:CDS 35.0%
! ATATTCCATGATTTTCCAGG+AGG + chr4_3:37794770-37794789 Msa0640130:CDS 35.0%
ATCGGATCAAACTGAAAAGT+CGG + chr4_3:37794931-37794950 Msa0640130:CDS 35.0%
! AAACTGAAAAGTCGGTTCAA+GGG + chr4_3:37794939-37794958 Msa0640130:CDS 35.0%
TACCAACATGATAACCATCT+GGG - chr4_3:37795233-37795252 None:intergenic 35.0%
TTACCAACATGATAACCATC+TGG - chr4_3:37795234-37795253 None:intergenic 35.0%
! GTCAATGCTTTCGAGTAAAT+TGG + chr4_3:37795255-37795274 Msa0640130:CDS 35.0%
! TTTTGAACGAAACAGAACTC+CGG - chr4_3:37795399-37795418 None:intergenic 35.0%
TAGTTTATTCCATAGGGAGA+TGG - chr4_3:37795541-37795560 None:intergenic 35.0%
!! TTGAGAGTCGACTTGAAATA+TGG + chr4_3:37795894-37795913 Msa0640130:intron 35.0%
!!! TCGTGTTTTTTGAAAGGTGT+AGG - chr4_3:37796348-37796367 None:intergenic 35.0%
GAAATTTCCATCGAAATCAG+CGG + chr4_3:37796366-37796385 Msa0640130:CDS 35.0%
AAATGGGAGTAGTAGTAGTA+AGG - chr4_3:37796435-37796454 None:intergenic 35.0%
CCACACAAAGAATCATTGTA+TGG - chr4_3:37796463-37796482 None:intergenic 35.0%
CCATACAATGATTCTTTGTG+TGG + chr4_3:37796460-37796479 Msa0640130:CDS 35.0%
TTCAAGTGGTTCTTCTCAAA+AGG + chr4_3:37796483-37796502 Msa0640130:CDS 35.0%
TCAAGTGGTTCTTCTCAAAA+GGG + chr4_3:37796484-37796503 Msa0640130:CDS 35.0%
AACATTTGCAAGGAATGCAA+TGG + chr4_3:37796587-37796606 Msa0640130:CDS 35.0%
! AAAGCATCTGGTCATAGTTA+TGG + chr4_3:37796676-37796695 Msa0640130:CDS 35.0%
ACTAGTCATGAACATAGCTT+TGG - chr4_3:37796712-37796731 None:intergenic 35.0%
TGTGTGTTGTACTGCTTAAT+TGG + chr4_3:37796801-37796820 Msa0640130:three_prime_UTR 35.0%
TTGTACTGCTTAATTGGTGT+TGG + chr4_3:37796807-37796826 Msa0640130:three_prime_UTR 35.0%
GTGTTGGAATGGATACAACA+AGG - chr4_3:37793243-37793262 None:intergenic 40.0%
GGGTTATAGTTGTGTTGGAA+TGG - chr4_3:37793254-37793273 None:intergenic 40.0%
AAAACCAAGGAAAAGGGTTG+TGG - chr4_3:37793309-37793328 None:intergenic 40.0%
AACAAAAATGGGGATGGAAG+AGG - chr4_3:37793414-37793433 None:intergenic 40.0%
TGGAGAAGAAACTTTCATGG+TGG + chr4_3:37793527-37793546 Msa0640130:CDS 40.0%
!! TTTCACTTTCTAGCTACCCT+TGG + chr4_3:37794034-37794053 Msa0640130:intron 40.0%
CACCTAAATAGCAGTGAAGT+GGG + chr4_3:37794099-37794118 Msa0640130:intron 40.0%
ACCTAAATAGCAGTGAAGTG+GGG + chr4_3:37794100-37794119 Msa0640130:intron 40.0%
GTGGTTAGTGAAAGTAGTGA+AGG - chr4_3:37794159-37794178 None:intergenic 40.0%
CACATTTAATGCTGCTGCTA+TGG + chr4_3:37794245-37794264 Msa0640130:intron 40.0%
TAGAATGAGGGTGAGTATCA+AGG - chr4_3:37794417-37794436 None:intergenic 40.0%
! CAAACTGAAAAGTCGGTTCA+AGG + chr4_3:37794938-37794957 Msa0640130:CDS 40.0%
!! AAGGGATTAGCTATTGGTCA+TGG + chr4_3:37794957-37794976 Msa0640130:CDS 40.0%
! GATTAGCTATTGGTCATGGT+CGG + chr4_3:37794961-37794980 Msa0640130:CDS 40.0%
!! TGTGATGCTAGAGAAGTGTT+TGG + chr4_3:37795036-37795055 Msa0640130:CDS 40.0%
AAGTGTTTGGATGCTGTAGT+TGG + chr4_3:37795049-37795068 Msa0640130:CDS 40.0%
AGTGTTTGGATGCTGTAGTT+GGG + chr4_3:37795050-37795069 Msa0640130:CDS 40.0%
! TTCGAACAACTTGATGGACA+CGG - chr4_3:37795103-37795122 None:intergenic 40.0%
!!! CTTGTTTTTAACTCCCCAGA+TGG + chr4_3:37795216-37795235 Msa0640130:CDS 40.0%
ACCAACATGATAACCATCTG+GGG - chr4_3:37795232-37795251 None:intergenic 40.0%
CTTCTCATCTGTATTAGCAG+CGG - chr4_3:37795332-37795351 None:intergenic 40.0%
!!! GATTTTTGAAAGCGTTCCTG+CGG + chr4_3:37795587-37795606 Msa0640130:CDS 40.0%
AAAGAATCCCTAGCAGAATC+TGG - chr4_3:37795742-37795761 None:intergenic 40.0%
TGATGAACTCTACCATGCAA+TGG + chr4_3:37795762-37795781 Msa0640130:CDS 40.0%
!!! TAGTTTTGTAGGGTCGTGTT+AGG + chr4_3:37795966-37795985 Msa0640130:intron 40.0%
GGATGTACCTATAACAGAAG+AGG - chr4_3:37796253-37796272 None:intergenic 40.0%
GAACGGATAAAGATATGCTG+TGG + chr4_3:37796289-37796308 Msa0640130:CDS 40.0%
GATTCTTTGTGTGGTTCAAG+TGG + chr4_3:37796469-37796488 Msa0640130:CDS 40.0%
GAACAAGTTGTGTTGTATGC+TGG + chr4_3:37796526-37796545 Msa0640130:CDS 40.0%
ATTTGCAAGGAATGCAATGG+AGG + chr4_3:37796590-37796609 Msa0640130:CDS 40.0%
TTTGCAAGGAATGCAATGGA+GGG + chr4_3:37796591-37796610 Msa0640130:CDS 40.0%
!! ACTGCTTAATTGGTGTTGGT+TGG + chr4_3:37796811-37796830 Msa0640130:three_prime_UTR 40.0%
!! CTGCTTAATTGGTGTTGGTT+GGG + chr4_3:37796812-37796831 Msa0640130:three_prime_UTR 40.0%
CGGTTGGGTTATAGTTGTGT+TGG - chr4_3:37793259-37793278 None:intergenic 45.0%
GGAAAAGGGTTGTGGTCAAT+TGG - chr4_3:37793301-37793320 None:intergenic 45.0%
! TTGACCACAACCCTTTTCCT+TGG + chr4_3:37793302-37793321 Msa0640130:five_prime_UTR 45.0%
AATGGGGATGGAAGAGGAAA+AGG - chr4_3:37793408-37793427 None:intergenic 45.0%
CGGTAGATTCGTCGAAATCA+TGG + chr4_3:37793587-37793606 Msa0640130:intron 45.0%
GCACCTAAATAGCAGTGAAG+TGG + chr4_3:37794098-37794117 Msa0640130:intron 45.0%
! GGAAAATCTAGTGGTGGAAG+TGG + chr4_3:37794734-37794753 Msa0640130:CDS 45.0%
!! CGGTTCAAGGGATTAGCTAT+TGG + chr4_3:37794951-37794970 Msa0640130:CDS 45.0%
CTGTCGTTCGAACAACTTGA+TGG - chr4_3:37795109-37795128 None:intergenic 45.0%
AGTTGTTCGAACGACAGTTC+AGG + chr4_3:37795112-37795131 Msa0640130:CDS 45.0%
GTTGTTCGAACGACAGTTCA+GGG + chr4_3:37795113-37795132 Msa0640130:CDS 45.0%
CGAATTTCACTCGTTCGACA+TGG + chr4_3:37795179-37795198 Msa0640130:CDS 45.0%
ATTTCACTCGTTCGACATGG+TGG + chr4_3:37795182-37795201 Msa0640130:CDS 45.0%
TCCCCAGATGGTTATCATGT+TGG + chr4_3:37795228-37795247 Msa0640130:CDS 45.0%
TTGCTTGTTCCATCTCCCTA+TGG + chr4_3:37795529-37795548 Msa0640130:CDS 45.0%
AGCAACTTTCCGCATCTGAA+CGG - chr4_3:37795635-37795654 None:intergenic 45.0%
AGCTCTTCCAGATTCTGCTA+GGG + chr4_3:37795732-37795751 Msa0640130:CDS 45.0%
CGAGATACATGTCCATTGCA+TGG - chr4_3:37795777-37795796 None:intergenic 45.0%
TGCAATGGACATGTATCTCG+AGG + chr4_3:37795777-37795796 Msa0640130:CDS 45.0%
GTAAAACATCGTCACGCTGT+TGG - chr4_3:37796118-37796137 None:intergenic 45.0%
CTCAACTCTCACAAGACGAA+CGG + chr4_3:37796272-37796291 Msa0640130:CDS 45.0%
AGGAATGCAATGGAGGGTTA+TGG + chr4_3:37796597-37796616 Msa0640130:CDS 45.0%
GAGACAGAAGTAGTGCGGTT+GGG - chr4_3:37793274-37793293 None:intergenic 50.0%
AGAGACAGAAGTAGTGCGGT+TGG - chr4_3:37793275-37793294 None:intergenic 50.0%
GTGCAGAGACAGAAGTAGTG+CGG - chr4_3:37793279-37793298 None:intergenic 50.0%
! GCCCCACTTCACTGCTATTT+AGG - chr4_3:37794104-37794123 None:intergenic 50.0%
CTGCACCTCCTGGAAAATCA+TGG - chr4_3:37794778-37794797 None:intergenic 50.0%
!! GATTTTCCAGGAGGTGCAGA+AGG + chr4_3:37794779-37794798 Msa0640130:CDS 50.0%
TCAAAACCTTCTGCACCTCC+TGG - chr4_3:37794788-37794807 None:intergenic 50.0%
! GCGTTGTGCTGCTGAATACA+TGG + chr4_3:37794874-37794893 Msa0640130:CDS 50.0%
GTTTGGATGCTGTAGTTGGG+AGG + chr4_3:37795053-37795072 Msa0640130:CDS 50.0%
!! TGTAGTTGGGAGGCTGGTTT+CGG + chr4_3:37795063-37795082 Msa0640130:CDS 50.0%
CGAACGACAGTTCAGGGATT+AGG + chr4_3:37795119-37795138 Msa0640130:CDS 50.0%
GCAACTTTCCGCATCTGAAC+GGG - chr4_3:37795634-37795653 None:intergenic 50.0%
CAGCTCTTCCAGATTCTGCT+AGG + chr4_3:37795731-37795750 Msa0640130:CDS 50.0%
CGTTCGTCTTGTGAGAGTTG+AGG - chr4_3:37796274-37796293 None:intergenic 50.0%
! GAAAGGTGTAGGCATGCTTG+AGG - chr4_3:37796337-37796356 None:intergenic 50.0%
!! GCTTGAACCGCTGATTTCGA+TGG - chr4_3:37796376-37796395 None:intergenic 50.0%
CGCGCGAGTAACAGATTCGA+AGG - chr4_3:37794857-37794876 None:intergenic 55.0%
!! TTGAAAGCGTTCCTGCGGCA+AGG + chr4_3:37795592-37795611 Msa0640130:CDS 55.0%
GGATGCTGTAGTTGGGAGGC+TGG + chr4_3:37795057-37795076 Msa0640130:CDS 60.0%
CGGTTGCTCCCGTTCAGATG+CGG + chr4_3:37795623-37795642 Msa0640130:CDS 60.0%
GCGTTCCTGCGGCAAGGAAC+TGG + chr4_3:37795598-37795617 Msa0640130:CDS 65.0%
CCTGCGGCAAGGAACTGGTG+CGG + chr4_3:37795603-37795622 Msa0640130:CDS 65.0%
CCGCACCAGTTCCTTGCCGC+AGG - chr4_3:37795606-37795625 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 37793170 37796892 37793170 ID=Msa0640130;Name=Msa0640130
chr4_3 mRNA 37793170 37796892 37793170 ID=Msa0640130-mRNA-1;Parent=Msa0640130;Name=Msa0640130-mRNA-1;_AED=0.15;_eAED=0.15;_QI=319|1|1|1|1|1|3|166|544
chr4_3 exon 37793170 37793554 37793170 ID=Msa0640130-mRNA-1:exon:8626;Parent=Msa0640130-mRNA-1
chr4_3 exon 37794692 37795798 37794692 ID=Msa0640130-mRNA-1:exon:8627;Parent=Msa0640130-mRNA-1
chr4_3 exon 37796265 37796892 37796265 ID=Msa0640130-mRNA-1:exon:8628;Parent=Msa0640130-mRNA-1
chr4_3 five_prime_UTR 37793170 37793488 37793170 ID=Msa0640130-mRNA-1:five_prime_utr;Parent=Msa0640130-mRNA-1
chr4_3 CDS 37793489 37793554 37793489 ID=Msa0640130-mRNA-1:cds;Parent=Msa0640130-mRNA-1
chr4_3 CDS 37794692 37795798 37794692 ID=Msa0640130-mRNA-1:cds;Parent=Msa0640130-mRNA-1
chr4_3 CDS 37796265 37796726 37796265 ID=Msa0640130-mRNA-1:cds;Parent=Msa0640130-mRNA-1
chr4_3 three_prime_UTR 37796727 37796892 37796727 ID=Msa0640130-mRNA-1:three_prime_utr;Parent=Msa0640130-mRNA-1
Gene Sequence

>Msa0640130

ATGGAAGAAGGTTTCTGTAATCTTGAAGTGGATGTAAATGGAGAAGAAACTTTCATGGTGGATAAGACGATTATTACTCAATACTCGAGCAAATTGGCTAAAGTATTTGGAAAATCTAGTGGTGGAAGTGGAAAGCTTAAAGTAATATTCCATGATTTTCCAGGAGGTGCAGAAGGTTTTGAGCTTATGTTAAGATTCTGTTATAACAATGGAAAAGGTAATTTAAGTCCTTCGAATCTGTTACTCGCGCGTTGTGCTGCTGAATACATGGAAATGAATGAATCTGTGAAAAATGTTTCTAACTTATCGGATCAAACTGAAAAGTCGGTTCAAGGGATTAGCTATTGGTCATGGTCGGAAATTTTGATTGCTTTGAAGCAATGCCAGAATTTGCTAGTTGATGATTCTTCTGTGATGCTAGAGAAGTGTTTGGATGCTGTAGTTGGGAGGCTGGTTTCGGCGAGTGAAGCAAGTCCGTGTCCATCAAGTTGTTCGAACGACAGTTCAGGGATTAGGTTTTCGTGTGACTCGAAAAGTACTGAGAGTATAAAGACGAATTTCACTCGTTCGACATGGTGGTTTGAAGATCTCTTGTTTTTAACTCCCCAGATGGTTATCATGTTGGTAAAGTCAATGCTTTCGAGTAAATTGGATCATTATGTTATAAGTAGGTTTCTTCTGTATTATCAGAAGGCGAAATTCACCGCTGCTAATACAGATGAGAAGTGCAAAATCATTGAAATGGTCATAGATATGCATTACGAAATAGACCGGAGTTCTGTTTCGTTCAAAACATTATTTGGGATTCTTCGGGTGACTTTGTGTTTGAACATAAGTAAATGTAGTAGGAATAAGTTGGAGATTATGATTGGTTCACAATTGGATCAAGTAACATTGGACAATTTGCTTGTTCCATCTCCCTATGGAATAAACTACTTATATGATGTGAACCTTGTTTTGAGATTTTTGAAAGCGTTCCTGCGGCAAGGAACTGGTGCGGTTGCTCCCGTTCAGATGCGGAAAGTTGCTAGCTTAATAGATTTGTATATAGCTGAAATTGCACCTGATCCTTGTTTAAAAACTTCAAAGTTTTTGGCTCTTGCTACAGCTCTTCCAGATTCTGCTAGGGATTCTTTTGATGAACTCTACCATGCAATGGACATGTATCTCGAGGTACATCCTCAACTCTCACAAGACGAACGGATAAAGATATGCTGTGGTTTAAATTATGATAAACTTTCACCTCAAGCATGCCTACACCTTTCAAAAAACACGAAATTTCCATCGAAATCAGCGGTTCAAGCTCTCATATCTCAACAATCTAAGCTAAAAAATTTACTCCTTACTACTACTACTCCCATTTCAACTCCATACAATGATTCTTTGTGTGGTTCAAGTGGTTCTTCTCAAAAGGGAAAGAAAGAAATAACCAATGAACAAGTTGTGTTGTATGCTGGAAATTTCGATATTCAACCAGATAATGAGAAAATGAAAGAACATTTGCAAGGAATGCAATGGAGGGTTATGGAATTAGAGAAAGTATGTAAGAAAATGCAAACTCAGATTGTTAAGATGACAAAATCAAAAGCATCTGGTCATAGTTATGGTAAATCTTTGCCAAAGCTATGTTCATGA
Protein sequence

>Msa0640130

MEEGFCNLEVDVNGEETFMVDKTIITQYSSKLAKVFGKSSGGSGKLKVIFHDFPGGAEGFELMLRFCYNNGKGNLSPSNLLLARCAAEYMEMNESVKNVSNLSDQTEKSVQGISYWSWSEILIALKQCQNLLVDDSSVMLEKCLDAVVGRLVSASEASPCPSSCSNDSSGIRFSCDSKSTESIKTNFTRSTWWFEDLLFLTPQMVIMLVKSMLSSKLDHYVISRFLLYYQKAKFTAANTDEKCKIIEMVIDMHYEIDRSSVSFKTLFGILRVTLCLNISKCSRNKLEIMIGSQLDQVTLDNLLVPSPYGINYLYDVNLVLRFLKAFLRQGTGAVAPVQMRKVASLIDLYIAEIAPDPCLKTSKFLALATALPDSARDSFDELYHAMDMYLEVHPQLSQDERIKICCGLNYDKLSPQACLHLSKNTKFPSKSAVQALISQQSKLKNLLLTTTTPISTPYNDSLCGSSGSSQKGKKEITNEQVVLYAGNFDIQPDNEKMKEHLQGMQWRVMELEKVCKKMQTQIVKMTKSKASGHSYGKSLPKLCS*