AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0679010

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 40 sgRNAs with CRISPR-Local

Find 190 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAATTTCATATTTCATATTT+TGG 0.098230 4_4:+36382083 Msa0679010:three_prime_UTR
TACTTGTGTAGTCATCTTAA+TGG 0.239207 4_4:+36381648 Msa0679010:CDS
TCTTTCTTAAGTTTATTCTA+AGG 0.277033 4_4:+36380404 Msa0679010:five_prime_UTR
GAATCTCAAAAGGGTGTATT+AGG 0.309355 4_4:-36380210 None:intergenic
TTTGGCACAACCCAAGATAT+AGG 0.377898 4_4:+36382101 Msa0679010:three_prime_UTR
AGAAACCTACACAGACCTAT+TGG 0.422731 4_4:+36380516 Msa0679010:CDS
CGAGTTCATGCTTATGATGC+AGG 0.425509 4_4:-36381820 None:intergenic
TCATTCGTGTTTGTTGTACT+TGG 0.431754 4_4:-36381680 None:intergenic
AAATGTTCGTATTTACTAAG+AGG 0.436990 4_4:-36382027 None:intergenic
CTTTCTTAAGTTTATTCTAA+GGG 0.442764 4_4:+36380405 Msa0679010:five_prime_UTR
TCACATTTGCTGCATCCAAT+AGG 0.445330 4_4:-36380531 None:intergenic
CAAACACGAATGAAGGTTAT+CGG 0.449915 4_4:+36381690 Msa0679010:exon
CTTGCAATCTTCCTCCTTGT+GGG 0.452655 4_4:-36381723 None:intergenic
GTTAAATTTCTACTATAAGA+AGG 0.455725 4_4:+36380335 Msa0679010:five_prime_UTR
TCGTGTGCAGGAATCTCAAA+AGG 0.457584 4_4:-36380220 None:intergenic
ACAGTGAACTTAATAGTGAT+AGG 0.462393 4_4:+36380259 Msa0679010:five_prime_UTR
AAACAGAAGTCACCCTTGAA+TGG 0.471611 4_4:-36381762 None:intergenic
TTAGAATAAACTTAAGAAAG+AGG 0.493228 4_4:-36380402 None:intergenic
TAGCTTGCCTCTTTGTTCGA+TGG 0.494054 4_4:-36381976 None:intergenic
CTTAAGTTTATTCTAAGGGT+TGG 0.497357 4_4:+36380409 Msa0679010:five_prime_UTR
AAAACTAAATTATCGTGTGC+AGG 0.500949 4_4:-36380232 None:intergenic
TACGAACATTTGAAAGATAA+CGG 0.501225 4_4:+36382039 Msa0679010:three_prime_UTR
CAAAGACAAGAAATGTAGTA+AGG 0.520318 4_4:+36380461 Msa0679010:CDS
AGTACAACAAACACGAATGA+AGG 0.521572 4_4:+36381683 Msa0679010:CDS
GCTTGCAATCTTCCTCCTTG+TGG 0.523056 4_4:-36381724 None:intergenic
TACTTATAAATCAGGATCAA+AGG 0.540549 4_4:+36381618 Msa0679010:intron
CGTAATAAAGCACAACGCAA+AGG 0.591463 4_4:+36380591 Msa0679010:CDS
TGCATCCAATAGGTCTGTGT+AGG 0.596040 4_4:-36380521 None:intergenic
CGTGTGCAGGAATCTCAAAA+GGG 0.608513 4_4:-36380219 None:intergenic
AGTAGAAATTTAACTGCACA+TGG 0.623938 4_4:-36380326 None:intergenic
AAGCAAGCCATCGAACAAAG+AGG 0.629726 4_4:+36381969 Msa0679010:three_prime_UTR
AGACAAGAAATGTAGTAAGG+AGG 0.650482 4_4:+36380464 Msa0679010:CDS
GAGTTCATGCTTATGATGCA+GGG 0.655127 4_4:-36381819 None:intergenic
TTATAAATCAGGATCAAAGG+TGG 0.655580 4_4:+36381621 Msa0679010:intron
CTAAAATAAATGTCAAACAG+AGG 0.660592 4_4:+36381940 Msa0679010:three_prime_UTR
GTTGGTGACAGAATCTTGTG+AGG 0.692312 4_4:-36381906 None:intergenic
TCGGAAAGTTCATACCCACA+AGG 0.731650 4_4:+36381709 Msa0679010:three_prime_UTR
GGGTGTATTAGGCAGAACGA+TGG 0.735268 4_4:-36380199 None:intergenic
GAAAGTTCATACCCACAAGG+AGG 0.739568 4_4:+36381712 Msa0679010:three_prime_UTR
TTCATGCTTATGATGCAGGG+AGG 0.744250 4_4:-36381816 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTAAAATAAATTTTGATTC+AGG + chr4_4:36381023-36381042 Msa0679010:intron 10.0%
!!! ATTAAAATAAATTTTGATTC+AGG + chr4_4:36381023-36381042 Msa0679010:intron 10.0%
!!! ATTAAAATAAATTTTGATTC+AGG + chr4_4:36381023-36381042 Msa0679010:intron 10.0%
!!! ATTAAAATAAATTTTGATTC+AGG + chr4_4:36381023-36381042 Msa0679010:intron 10.0%
!!! AATTTTAAAAAATAGTGAGA+TGG + chr4_4:36380698-36380717 Msa0679010:intron 15.0%
!!! TTACTTTGAAGATATAAAAA+AGG + chr4_4:36380998-36381017 Msa0679010:intron 15.0%
!!! TTACTTTGAAGATATAAAAA+AGG + chr4_4:36380998-36381017 Msa0679010:intron 15.0%
!!! TTTTGATACTTAATTTACTA+TGG - chr4_4:36381426-36381445 None:intergenic 15.0%
!! TAAAAAAATCTTATTCAATC+AGG + chr4_4:36381866-36381885 Msa0679010:three_prime_UTR 15.0%
!!! GAAATATGAAATTCTATTTT+TGG - chr4_4:36382077-36382096 None:intergenic 15.0%
!!! GAATTTCATATTTCATATTT+TGG + chr4_4:36382083-36382102 Msa0679010:three_prime_UTR 15.0%
!!! AATTTTAAAAAATAGTGAGA+TGG + chr4_4:36380698-36380717 Msa0679010:intron 15.0%
!!! TTACTTTGAAGATATAAAAA+AGG + chr4_4:36380998-36381017 Msa0679010:intron 15.0%
!!! TTACTTTGAAGATATAAAAA+AGG + chr4_4:36380998-36381017 Msa0679010:intron 15.0%
!!! TTTTGATACTTAATTTACTA+TGG - chr4_4:36381426-36381445 None:intergenic 15.0%
!! TAAAAAAATCTTATTCAATC+AGG + chr4_4:36381866-36381885 Msa0679010:three_prime_UTR 15.0%
!!! GAAATATGAAATTCTATTTT+TGG - chr4_4:36382077-36382096 None:intergenic 15.0%
!!! GAATTTCATATTTCATATTT+TGG + chr4_4:36382083-36382102 Msa0679010:three_prime_UTR 15.0%
!! GTTAAATTTCTACTATAAGA+AGG + chr4_4:36380335-36380354 Msa0679010:five_prime_UTR 20.0%
!! TATGAAAAAGTTATTGATGT+TGG - chr4_4:36380375-36380394 None:intergenic 20.0%
!! TTAGAATAAACTTAAGAAAG+AGG - chr4_4:36380405-36380424 None:intergenic 20.0%
!! TCTTTCTTAAGTTTATTCTA+AGG + chr4_4:36380404-36380423 Msa0679010:five_prime_UTR 20.0%
!! CTTTCTTAAGTTTATTCTAA+GGG + chr4_4:36380405-36380424 Msa0679010:five_prime_UTR 20.0%
!!! ATCCTTTTAATGATTGTAAT+TGG + chr4_4:36380618-36380637 Msa0679010:intron 20.0%
!!! TCCTTTTAATGATTGTAATT+GGG + chr4_4:36380619-36380638 Msa0679010:intron 20.0%
!!! CACATAATAGTTTTTTTAAG+AGG + chr4_4:36380655-36380674 Msa0679010:intron 20.0%
!! AAAAGAATTAGTAAGTAACA+AGG + chr4_4:36380803-36380822 Msa0679010:intron 20.0%
!!! TATTCGTGAAGTATTATATT+AGG - chr4_4:36380884-36380903 None:intergenic 20.0%
!!! TAAAATGAAAGTGTTATGTT+TGG - chr4_4:36380955-36380974 None:intergenic 20.0%
!! TTGTCAATTAAAGTAATTAC+AGG - chr4_4:36381192-36381211 None:intergenic 20.0%
!! TTTCATACGTATAAAAACAT+AGG - chr4_4:36381241-36381260 None:intergenic 20.0%
!!! TTTATACCCTTTTTTCTATT+AGG + chr4_4:36381395-36381414 Msa0679010:intron 20.0%
!! ATACTTAATTTACTATGGTA+TGG - chr4_4:36381421-36381440 None:intergenic 20.0%
!! TCAAAAACAAAAAATCTTTC+CGG + chr4_4:36381440-36381459 Msa0679010:intron 20.0%
!! ATAGTTCTTACTTATAAATC+AGG + chr4_4:36381610-36381629 Msa0679010:intron 20.0%
!! GTTAAATTTCTACTATAAGA+AGG + chr4_4:36380335-36380354 Msa0679010:five_prime_UTR 20.0%
!! TATGAAAAAGTTATTGATGT+TGG - chr4_4:36380375-36380394 None:intergenic 20.0%
!! TTAGAATAAACTTAAGAAAG+AGG - chr4_4:36380405-36380424 None:intergenic 20.0%
!! TCTTTCTTAAGTTTATTCTA+AGG + chr4_4:36380404-36380423 Msa0679010:five_prime_UTR 20.0%
!! CTTTCTTAAGTTTATTCTAA+GGG + chr4_4:36380405-36380424 Msa0679010:five_prime_UTR 20.0%
!!! ATCCTTTTAATGATTGTAAT+TGG + chr4_4:36380618-36380637 Msa0679010:intron 20.0%
!!! TCCTTTTAATGATTGTAATT+GGG + chr4_4:36380619-36380638 Msa0679010:intron 20.0%
!!! CACATAATAGTTTTTTTAAG+AGG + chr4_4:36380655-36380674 Msa0679010:intron 20.0%
!! AAAAGAATTAGTAAGTAACA+AGG + chr4_4:36380803-36380822 Msa0679010:intron 20.0%
!!! TATTCGTGAAGTATTATATT+AGG - chr4_4:36380884-36380903 None:intergenic 20.0%
!!! TAAAATGAAAGTGTTATGTT+TGG - chr4_4:36380955-36380974 None:intergenic 20.0%
!! TTGTCAATTAAAGTAATTAC+AGG - chr4_4:36381192-36381211 None:intergenic 20.0%
!! TTTCATACGTATAAAAACAT+AGG - chr4_4:36381241-36381260 None:intergenic 20.0%
!!! TTTATACCCTTTTTTCTATT+AGG + chr4_4:36381395-36381414 Msa0679010:intron 20.0%
!! ATACTTAATTTACTATGGTA+TGG - chr4_4:36381421-36381440 None:intergenic 20.0%
!! TCAAAAACAAAAAATCTTTC+CGG + chr4_4:36381440-36381459 Msa0679010:intron 20.0%
!! ATAGTTCTTACTTATAAATC+AGG + chr4_4:36381610-36381629 Msa0679010:intron 20.0%
!! AAAAATGGAGAATGTTTTTC+AGG + chr4_4:36380434-36380453 Msa0679010:exon 25.0%
! ACCCAATTACAATCATTAAA+AGG - chr4_4:36380623-36380642 None:intergenic 25.0%
!!! TAGTGAGATGGATTTTAAAA+AGG + chr4_4:36380710-36380729 Msa0679010:intron 25.0%
!!! AGTGAGATGGATTTTAAAAA+GGG + chr4_4:36380711-36380730 Msa0679010:intron 25.0%
! AAATCTAAGGGAATAAAGAA+TGG - chr4_4:36381338-36381357 None:intergenic 25.0%
! ATCTTTAATGATACTTCAGT+GGG - chr4_4:36381368-36381387 None:intergenic 25.0%
! AATCTTTAATGATACTTCAG+TGG - chr4_4:36381369-36381388 None:intergenic 25.0%
!! AGAAAAAAGGGTATAAAGAA+AGG - chr4_4:36381392-36381411 None:intergenic 25.0%
! TACTTATAAATCAGGATCAA+AGG + chr4_4:36381618-36381637 Msa0679010:intron 25.0%
!!! ATTTTAGGCTTTGAATAAGT+TGG - chr4_4:36381927-36381946 None:intergenic 25.0%
!!! CTCTGTTTGACATTTATTTT+AGG - chr4_4:36381942-36381961 None:intergenic 25.0%
! CTAAAATAAATGTCAAACAG+AGG + chr4_4:36381940-36381959 Msa0679010:three_prime_UTR 25.0%
! AAATGTTCGTATTTACTAAG+AGG - chr4_4:36382030-36382049 None:intergenic 25.0%
! TACGAACATTTGAAAGATAA+CGG + chr4_4:36382039-36382058 Msa0679010:three_prime_UTR 25.0%
!!! TTCTATTTTTGGTAATAGAG+TGG - chr4_4:36382066-36382085 None:intergenic 25.0%
!!! ATTTTGATGTCCTATATCTT+GGG - chr4_4:36382114-36382133 None:intergenic 25.0%
!!! AATTTTGATGTCCTATATCT+TGG - chr4_4:36382115-36382134 None:intergenic 25.0%
!! AAAAATGGAGAATGTTTTTC+AGG + chr4_4:36380434-36380453 Msa0679010:exon 25.0%
! ACCCAATTACAATCATTAAA+AGG - chr4_4:36380623-36380642 None:intergenic 25.0%
!!! TAGTGAGATGGATTTTAAAA+AGG + chr4_4:36380710-36380729 Msa0679010:intron 25.0%
!!! AGTGAGATGGATTTTAAAAA+GGG + chr4_4:36380711-36380730 Msa0679010:intron 25.0%
! AAATCTAAGGGAATAAAGAA+TGG - chr4_4:36381338-36381357 None:intergenic 25.0%
! ATCTTTAATGATACTTCAGT+GGG - chr4_4:36381368-36381387 None:intergenic 25.0%
! AATCTTTAATGATACTTCAG+TGG - chr4_4:36381369-36381388 None:intergenic 25.0%
!! AGAAAAAAGGGTATAAAGAA+AGG - chr4_4:36381392-36381411 None:intergenic 25.0%
! TACTTATAAATCAGGATCAA+AGG + chr4_4:36381618-36381637 Msa0679010:intron 25.0%
!!! ATTTTAGGCTTTGAATAAGT+TGG - chr4_4:36381927-36381946 None:intergenic 25.0%
!!! CTCTGTTTGACATTTATTTT+AGG - chr4_4:36381942-36381961 None:intergenic 25.0%
! CTAAAATAAATGTCAAACAG+AGG + chr4_4:36381940-36381959 Msa0679010:three_prime_UTR 25.0%
! AAATGTTCGTATTTACTAAG+AGG - chr4_4:36382030-36382049 None:intergenic 25.0%
! TACGAACATTTGAAAGATAA+CGG + chr4_4:36382039-36382058 Msa0679010:three_prime_UTR 25.0%
!!! TTCTATTTTTGGTAATAGAG+TGG - chr4_4:36382066-36382085 None:intergenic 25.0%
!!! ATTTTGATGTCCTATATCTT+GGG - chr4_4:36382114-36382133 None:intergenic 25.0%
!!! AATTTTGATGTCCTATATCT+TGG - chr4_4:36382115-36382134 None:intergenic 25.0%
AAAACTAAATTATCGTGTGC+AGG - chr4_4:36380235-36380254 None:intergenic 30.0%
ACAGTGAACTTAATAGTGAT+AGG + chr4_4:36380259-36380278 Msa0679010:five_prime_UTR 30.0%
AACTTAATAGTGATAGGTTG+AGG + chr4_4:36380265-36380284 Msa0679010:intron 30.0%
AGTAGAAATTTAACTGCACA+TGG - chr4_4:36380329-36380348 None:intergenic 30.0%
! CTTAAGTTTATTCTAAGGGT+TGG + chr4_4:36380409-36380428 Msa0679010:five_prime_UTR 30.0%
CAAAGACAAGAAATGTAGTA+AGG + chr4_4:36380461-36380480 Msa0679010:CDS 30.0%
TAATGATTGTAATTGGGTCA+AGG + chr4_4:36380625-36380644 Msa0679010:intron 30.0%
!!! TGAAAGTAGTTTTGACAGAA+AGG + chr4_4:36380771-36380790 Msa0679010:intron 30.0%
AAGTAACAAGGAAAAGACTA+AGG + chr4_4:36380815-36380834 Msa0679010:intron 30.0%
TGAAACGTTATGCAAGTAAA+AGG + chr4_4:36380917-36380936 Msa0679010:intron 30.0%
GTATGGCCTAATAGAAAAAA+GGG - chr4_4:36381404-36381423 None:intergenic 30.0%
! AAAAATTCGTCTAAAGTTGC+CGG - chr4_4:36381462-36381481 None:intergenic 30.0%
TTATAAATCAGGATCAAAGG+TGG + chr4_4:36381621-36381640 Msa0679010:intron 30.0%
TACTTGTGTAGTCATCTTAA+TGG + chr4_4:36381648-36381667 Msa0679010:CDS 30.0%
!!! TCATTTTTGAGTTCCATTCA+AGG + chr4_4:36381749-36381768 Msa0679010:three_prime_UTR 30.0%
!!! CATTTTTGAGTTCCATTCAA+GGG + chr4_4:36381750-36381769 Msa0679010:three_prime_UTR 30.0%
AAAACTAAATTATCGTGTGC+AGG - chr4_4:36380235-36380254 None:intergenic 30.0%
ACAGTGAACTTAATAGTGAT+AGG + chr4_4:36380259-36380278 Msa0679010:five_prime_UTR 30.0%
AACTTAATAGTGATAGGTTG+AGG + chr4_4:36380265-36380284 Msa0679010:intron 30.0%
AGTAGAAATTTAACTGCACA+TGG - chr4_4:36380329-36380348 None:intergenic 30.0%
! CTTAAGTTTATTCTAAGGGT+TGG + chr4_4:36380409-36380428 Msa0679010:five_prime_UTR 30.0%
CAAAGACAAGAAATGTAGTA+AGG + chr4_4:36380461-36380480 Msa0679010:CDS 30.0%
TAATGATTGTAATTGGGTCA+AGG + chr4_4:36380625-36380644 Msa0679010:intron 30.0%
!!! TGAAAGTAGTTTTGACAGAA+AGG + chr4_4:36380771-36380790 Msa0679010:intron 30.0%
AAGTAACAAGGAAAAGACTA+AGG + chr4_4:36380815-36380834 Msa0679010:intron 30.0%
TGAAACGTTATGCAAGTAAA+AGG + chr4_4:36380917-36380936 Msa0679010:intron 30.0%
GTATGGCCTAATAGAAAAAA+GGG - chr4_4:36381404-36381423 None:intergenic 30.0%
! AAAAATTCGTCTAAAGTTGC+CGG - chr4_4:36381462-36381481 None:intergenic 30.0%
TTATAAATCAGGATCAAAGG+TGG + chr4_4:36381621-36381640 Msa0679010:intron 30.0%
TACTTGTGTAGTCATCTTAA+TGG + chr4_4:36381648-36381667 Msa0679010:CDS 30.0%
!!! TCATTTTTGAGTTCCATTCA+AGG + chr4_4:36381749-36381768 Msa0679010:three_prime_UTR 30.0%
!!! CATTTTTGAGTTCCATTCAA+GGG + chr4_4:36381750-36381769 Msa0679010:three_prime_UTR 30.0%
! GAATCTCAAAAGGGTGTATT+AGG - chr4_4:36380213-36380232 None:intergenic 35.0%
CTATCACTATTAAGTGCACA+TGG - chr4_4:36380295-36380314 None:intergenic 35.0%
CATGTGCACTTAATAGTGAT+AGG + chr4_4:36380293-36380312 Msa0679010:intron 35.0%
CACTTAATAGTGATAGGTTG+AGG + chr4_4:36380299-36380318 Msa0679010:intron 35.0%
!! TTCTAAGGGTTGGTGAAAAA+TGG + chr4_4:36380419-36380438 Msa0679010:exon 35.0%
AGACAAGAAATGTAGTAAGG+AGG + chr4_4:36380464-36380483 Msa0679010:CDS 35.0%
GGTATGGCCTAATAGAAAAA+AGG - chr4_4:36381405-36381424 None:intergenic 35.0%
!! TCATTCGTGTTTGTTGTACT+TGG - chr4_4:36381683-36381702 None:intergenic 35.0%
AGTACAACAAACACGAATGA+AGG + chr4_4:36381683-36381702 Msa0679010:CDS 35.0%
CAAACACGAATGAAGGTTAT+CGG + chr4_4:36381690-36381709 Msa0679010:exon 35.0%
! GAATCTCAAAAGGGTGTATT+AGG - chr4_4:36380213-36380232 None:intergenic 35.0%
CTATCACTATTAAGTGCACA+TGG - chr4_4:36380295-36380314 None:intergenic 35.0%
CATGTGCACTTAATAGTGAT+AGG + chr4_4:36380293-36380312 Msa0679010:intron 35.0%
CACTTAATAGTGATAGGTTG+AGG + chr4_4:36380299-36380318 Msa0679010:intron 35.0%
!! TTCTAAGGGTTGGTGAAAAA+TGG + chr4_4:36380419-36380438 Msa0679010:exon 35.0%
AGACAAGAAATGTAGTAAGG+AGG + chr4_4:36380464-36380483 Msa0679010:CDS 35.0%
GGTATGGCCTAATAGAAAAA+AGG - chr4_4:36381405-36381424 None:intergenic 35.0%
!! TCATTCGTGTTTGTTGTACT+TGG - chr4_4:36381683-36381702 None:intergenic 35.0%
AGTACAACAAACACGAATGA+AGG + chr4_4:36381683-36381702 Msa0679010:CDS 35.0%
CAAACACGAATGAAGGTTAT+CGG + chr4_4:36381690-36381709 Msa0679010:exon 35.0%
AGAAACCTACACAGACCTAT+TGG + chr4_4:36380516-36380535 Msa0679010:CDS 40.0%
! TCACATTTGCTGCATCCAAT+AGG - chr4_4:36380534-36380553 None:intergenic 40.0%
CGTAATAAAGCACAACGCAA+AGG + chr4_4:36380591-36380610 Msa0679010:CDS 40.0%
!!! AGTAGTTTTGACAGAAAGGC+AGG + chr4_4:36380775-36380794 Msa0679010:intron 40.0%
! TTTTGACAGAAAGGCAGGTA+AGG + chr4_4:36380780-36380799 Msa0679010:intron 40.0%
CGTTATGCAAGTAAAAGGTG+AGG + chr4_4:36380922-36380941 Msa0679010:intron 40.0%
GTGGGTGTGTAGAAATCTAA+GGG - chr4_4:36381350-36381369 None:intergenic 40.0%
AGTGGGTGTGTAGAAATCTA+AGG - chr4_4:36381351-36381370 None:intergenic 40.0%
AAACAGAAGTCACCCTTGAA+TGG - chr4_4:36381765-36381784 None:intergenic 40.0%
! AGGGTGACTTCTGTTTTCAA+AGG + chr4_4:36381769-36381788 Msa0679010:three_prime_UTR 40.0%
GAGTTCATGCTTATGATGCA+GGG - chr4_4:36381822-36381841 None:intergenic 40.0%
TTTGGCACAACCCAAGATAT+AGG + chr4_4:36382101-36382120 Msa0679010:three_prime_UTR 40.0%
AGAAACCTACACAGACCTAT+TGG + chr4_4:36380516-36380535 Msa0679010:CDS 40.0%
! TCACATTTGCTGCATCCAAT+AGG - chr4_4:36380534-36380553 None:intergenic 40.0%
CGTAATAAAGCACAACGCAA+AGG + chr4_4:36380591-36380610 Msa0679010:CDS 40.0%
!!! AGTAGTTTTGACAGAAAGGC+AGG + chr4_4:36380775-36380794 Msa0679010:intron 40.0%
! TTTTGACAGAAAGGCAGGTA+AGG + chr4_4:36380780-36380799 Msa0679010:intron 40.0%
CGTTATGCAAGTAAAAGGTG+AGG + chr4_4:36380922-36380941 Msa0679010:intron 40.0%
GTGGGTGTGTAGAAATCTAA+GGG - chr4_4:36381350-36381369 None:intergenic 40.0%
AGTGGGTGTGTAGAAATCTA+AGG - chr4_4:36381351-36381370 None:intergenic 40.0%
AAACAGAAGTCACCCTTGAA+TGG - chr4_4:36381765-36381784 None:intergenic 40.0%
! AGGGTGACTTCTGTTTTCAA+AGG + chr4_4:36381769-36381788 Msa0679010:three_prime_UTR 40.0%
GAGTTCATGCTTATGATGCA+GGG - chr4_4:36381822-36381841 None:intergenic 40.0%
TTTGGCACAACCCAAGATAT+AGG + chr4_4:36382101-36382120 Msa0679010:three_prime_UTR 40.0%
CGTGTGCAGGAATCTCAAAA+GGG - chr4_4:36380222-36380241 None:intergenic 45.0%
TCGTGTGCAGGAATCTCAAA+AGG - chr4_4:36380223-36380242 None:intergenic 45.0%
TGCATCCAATAGGTCTGTGT+AGG - chr4_4:36380524-36380543 None:intergenic 45.0%
TCGGAAAGTTCATACCCACA+AGG + chr4_4:36381709-36381728 Msa0679010:three_prime_UTR 45.0%
GAAAGTTCATACCCACAAGG+AGG + chr4_4:36381712-36381731 Msa0679010:three_prime_UTR 45.0%
CTTGCAATCTTCCTCCTTGT+GGG - chr4_4:36381726-36381745 None:intergenic 45.0%
TTCATGCTTATGATGCAGGG+AGG - chr4_4:36381819-36381838 None:intergenic 45.0%
CGAGTTCATGCTTATGATGC+AGG - chr4_4:36381823-36381842 None:intergenic 45.0%
!! GTTGGTGACAGAATCTTGTG+AGG - chr4_4:36381909-36381928 None:intergenic 45.0%
AAGCAAGCCATCGAACAAAG+AGG + chr4_4:36381969-36381988 Msa0679010:three_prime_UTR 45.0%
! TAGCTTGCCTCTTTGTTCGA+TGG - chr4_4:36381979-36381998 None:intergenic 45.0%
CGTGTGCAGGAATCTCAAAA+GGG - chr4_4:36380222-36380241 None:intergenic 45.0%
TCGTGTGCAGGAATCTCAAA+AGG - chr4_4:36380223-36380242 None:intergenic 45.0%
TGCATCCAATAGGTCTGTGT+AGG - chr4_4:36380524-36380543 None:intergenic 45.0%
TCGGAAAGTTCATACCCACA+AGG + chr4_4:36381709-36381728 Msa0679010:three_prime_UTR 45.0%
GAAAGTTCATACCCACAAGG+AGG + chr4_4:36381712-36381731 Msa0679010:three_prime_UTR 45.0%
CTTGCAATCTTCCTCCTTGT+GGG - chr4_4:36381726-36381745 None:intergenic 45.0%
TTCATGCTTATGATGCAGGG+AGG - chr4_4:36381819-36381838 None:intergenic 45.0%
CGAGTTCATGCTTATGATGC+AGG - chr4_4:36381823-36381842 None:intergenic 45.0%
!! GTTGGTGACAGAATCTTGTG+AGG - chr4_4:36381909-36381928 None:intergenic 45.0%
AAGCAAGCCATCGAACAAAG+AGG + chr4_4:36381969-36381988 Msa0679010:three_prime_UTR 45.0%
! TAGCTTGCCTCTTTGTTCGA+TGG - chr4_4:36381979-36381998 None:intergenic 45.0%
!!! ATTTTTTTAATTTGATTATA+TGG - chr4_4:36381855-36381874 None:intergenic 5.0%
!!! ATTTTTTTAATTTGATTATA+TGG - chr4_4:36381855-36381874 None:intergenic 5.0%
GGGTGTATTAGGCAGAACGA+TGG - chr4_4:36380202-36380221 None:intergenic 50.0%
GCTTGCAATCTTCCTCCTTG+TGG - chr4_4:36381727-36381746 None:intergenic 50.0%
GGGTGTATTAGGCAGAACGA+TGG - chr4_4:36380202-36380221 None:intergenic 50.0%
GCTTGCAATCTTCCTCCTTG+TGG - chr4_4:36381727-36381746 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 36380190 36382145 36380190 ID=Msa0679010;Name=Msa0679010
chr4_4 mRNA 36380190 36382145 36380190 ID=Msa0679010-mRNA-1;Parent=Msa0679010;Name=Msa0679010-mRNA-1;_AED=0.00;_eAED=0.00;_QI=214|1|1|1|0|0|3|443|81
chr4_4 exon 36380190 36380280 36380190 ID=Msa0679010-mRNA-1:exon:8534;Parent=Msa0679010-mRNA-1
chr4_4 exon 36380315 36380612 36380315 ID=Msa0679010-mRNA-1:exon:8535;Parent=Msa0679010-mRNA-1
chr4_4 exon 36381632 36382145 36381632 ID=Msa0679010-mRNA-1:exon:8536;Parent=Msa0679010-mRNA-1
chr4_4 five_prime_UTR 36380190 36380280 36380190 ID=Msa0679010-mRNA-1:five_prime_utr;Parent=Msa0679010-mRNA-1
chr4_4 five_prime_UTR 36380315 36380437 36380315 ID=Msa0679010-mRNA-1:five_prime_utr;Parent=Msa0679010-mRNA-1
chr4_4 CDS 36380438 36380612 36380438 ID=Msa0679010-mRNA-1:cds;Parent=Msa0679010-mRNA-1
chr4_4 CDS 36381632 36381702 36381632 ID=Msa0679010-mRNA-1:cds;Parent=Msa0679010-mRNA-1
chr4_4 three_prime_UTR 36381703 36382145 36381703 ID=Msa0679010-mRNA-1:three_prime_utr;Parent=Msa0679010-mRNA-1
Gene Sequence

>Msa0679010

ATGGAGAATGTTTTTCAGGAACACAAAGACAAGAAATGTAGTAAGGAGGAAAATCAGATGCAAATCTTACACAACACAAGAAACCTACACAGACCTATTGGATGCAGCAAATGTGAGTCGTACATCTCAAAATGCAAGTGCTCTACGAAGAATCGTAATAAAGCACAACGCAAAGGATCAAAGGTGGACAATACTTGTGTAGTCATCTTAATGGAAACAGTGTCCAAGTACAACAAACACGAATGA
Protein sequence

>Msa0679010

MENVFQEHKDKKCSKEENQMQILHNTRNLHRPIGCSKCESYISKCKCSTKNRNKAQRKGSKVDNTCVVILMETVSKYNKHE*