AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0687600

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0687600 MtrunA17_Chr4g0038301 89.439 303 9 4 1 297 1 286 0.0 521
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0687600 AT3G02820.1 45.424 295 125 8 8 290 6 276 2.90e-74 229
Msa0687600 AT3G02820.2 45.424 295 125 8 8 290 23 293 4.39e-74 229

Find 96 sgRNAs with CRISPR-Local

Find 202 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACAGACTGGTTCCATTATT+TGG 0.093724 4_4:+52500054 None:intergenic
TAGGGTTTGGATTGGGATTT+GGG 0.153645 4_4:+52502462 None:intergenic
CTTCTCAGTTGTGCTACTTT+TGG 0.158577 4_4:+52500092 None:intergenic
GCGGTGGAGGTGGTGGTGTT+AGG 0.206154 4_4:+52502443 None:intergenic
TTAGGGTTTGGATTGGGATT+TGG 0.242699 4_4:+52502461 None:intergenic
TCTCGAATGTGTATTGAAAA+TGG 0.265771 4_4:+52502595 None:intergenic
GGCATGTTTCGAAAGTAAAT+TGG 0.268301 4_4:-52499779 Msa0687600:three_prime_UTR
GAGGTGGTGGTGTTAGGGTT+TGG 0.285661 4_4:+52502449 None:intergenic
GTAACAACCTGTTGCTGTTC+CGG 0.287274 4_4:+52502532 None:intergenic
AGAAGCTCGGGAGTGAGTTT+TGG 0.293857 4_4:+52502341 None:intergenic
CTCTTTCTCTCAATTCTCTA+AGG 0.296516 4_4:+52501380 None:intergenic
TCAAATGAAGTCCCAAATAA+TGG 0.319473 4_4:-52500066 Msa0687600:CDS
AGGTGGATCGGCGGTGGAGG+TGG 0.322815 4_4:+52502433 None:intergenic
CTTCTCTCTGATGACGGCCT+TGG 0.344424 4_4:-52502320 Msa0687600:CDS
GTGGTGTTAGGGTTTGGATT+GGG 0.352807 4_4:+52502455 None:intergenic
ACAGACTGGTTCCATTATTT+GGG 0.352932 4_4:+52500055 None:intergenic
CGGTGGAGGTGGTGGTGTTA+GGG 0.379415 4_4:+52502444 None:intergenic
GTGATTGAATAGTTGACTTC+TGG 0.388969 4_4:-52499853 Msa0687600:three_prime_UTR
TGCTGTTATCTCAGCACTGC+TGG 0.389855 4_4:+52500032 None:intergenic
GGAGGTAGGTGGATCGGCGG+TGG 0.391333 4_4:+52502427 None:intergenic
AGATTACCTTGTTCACCATC+TGG 0.397013 4_4:+52501301 None:intergenic
TACATTGCAATCTTGAAGAT+TGG 0.397369 4_4:-52499800 Msa0687600:three_prime_UTR
GGTAGGTGGATCGGCGGTGG+AGG 0.397936 4_4:+52502430 None:intergenic
TAGCTGTATCTGCAGAAAAC+GGG 0.413562 4_4:-52500739 Msa0687600:CDS
TGCTGCTACTAGGCGTGTTA+AGG 0.413675 4_4:-52502050 Msa0687600:intron
TGGATCGGCGGTGGAGGTGG+TGG 0.423688 4_4:+52502436 None:intergenic
TCAGGATCCGGAACAGCAAC+AGG 0.431444 4_4:-52502539 Msa0687600:CDS
GGTGGTGTTAGGGTTTGGAT+TGG 0.441623 4_4:+52502454 None:intergenic
ATAGTTGACTTCTGGTGGTC+TGG 0.443524 4_4:-52499845 Msa0687600:three_prime_UTR
AGACTGTGGTATGCTGGCTT+TGG 0.446290 4_4:+52499954 None:intergenic
TTGCTAAGTATCCCTACATT+GGG 0.450737 4_4:-52500764 Msa0687600:CDS
TCAACCTATTTGCTTCCATA+CGG 0.452181 4_4:+52499998 None:intergenic
GAGCCCGTATGGAAGCAAAT+AGG 0.454008 4_4:-52500002 Msa0687600:CDS
CTCAGCACTGCTGGACAGAC+TGG 0.455206 4_4:+52500041 None:intergenic
TCTGGGATGTTGTCCACAAC+TGG 0.469784 4_4:+52501319 None:intergenic
GAGAAAGAGTTGCAAATGGC+GGG 0.470312 4_4:-52501366 Msa0687600:CDS
CTAGGAAGACTGTGGTATGC+TGG 0.471259 4_4:+52499948 None:intergenic
GAAGAGAAAATGTCAGGATC+CGG 0.473828 4_4:-52502551 Msa0687600:exon
ACCTCCACCTTCAAACCCTC+TGG 0.474558 4_4:-52502410 Msa0687600:CDS
CTCGAATGTGTATTGAAAAT+GGG 0.478274 4_4:+52502596 None:intergenic
TTAGCTGTATCTGCAGAAAA+CGG 0.478420 4_4:-52500740 Msa0687600:CDS
TTTGCTAAGTATCCCTACAT+TGG 0.480824 4_4:-52500765 Msa0687600:CDS
AGAAGAGAAGAGAAAATGTC+AGG 0.483482 4_4:-52502557 Msa0687600:exon
CAACCTATTTGCTTCCATAC+GGG 0.491149 4_4:+52499999 None:intergenic
AGCAGAGCAGAGAGCCCGTA+TGG 0.494494 4_4:-52500013 Msa0687600:CDS
TCTGGGTCCAGAGGGTTTGA+AGG 0.495023 4_4:+52502403 None:intergenic
TTGGGATTTGGGGCGGTGAA+AGG 0.495060 4_4:+52502473 None:intergenic
GTTTGGATTGGGATTTGGGG+CGG 0.497587 4_4:+52502466 None:intergenic
TTGAGAAAGTTGCTGCTACT+AGG 0.498945 4_4:-52502060 Msa0687600:CDS
TCATCAGAGAGAAGAAGCTC+GGG 0.503800 4_4:+52502329 None:intergenic
GTCATCAGAGAGAAGAAGCT+CGG 0.508978 4_4:+52502328 None:intergenic
GATTACCTTGTTCACCATCT+GGG 0.514063 4_4:+52501302 None:intergenic
TGTTACAAATGCGGCAGACC+AGG 0.517761 4_4:-52502515 Msa0687600:CDS
AGGGTTTGGATTGGGATTTG+GGG 0.518659 4_4:+52502463 None:intergenic
GATACAGCTAAACCCAATGT+AGG 0.525153 4_4:+52500752 None:intergenic
GGTTTGAAGGTGGAGGTAGG+TGG 0.525605 4_4:+52502416 None:intergenic
GAAGGTGGAGGTAGGTGGAT+CGG 0.539108 4_4:+52502421 None:intergenic
TATGGAAGCAAATAGGTTGA+AGG 0.541904 4_4:-52499995 Msa0687600:CDS
TGCTTGACTTATACTCTGAC+TGG 0.544504 4_4:-52502132 Msa0687600:CDS
ATAGGTTGAAGGCACTAGAA+AGG 0.545276 4_4:-52499984 Msa0687600:CDS
TTACAATGAATATCAAAGCT+AGG 0.548001 4_4:+52499930 None:intergenic
AACTTCAAATACCACGGTCG+CGG 0.560735 4_4:-52502272 Msa0687600:CDS
TGTTTCGAAAGTAAATTGGT+TGG 0.564878 4_4:-52499775 Msa0687600:three_prime_UTR
CCGTGGTATTTGAAGTTGCG+AGG 0.565970 4_4:+52502278 None:intergenic
CAGTCGCGTGACCAGTGACC+TGG 0.568375 4_4:+52502497 None:intergenic
GGTGGTTCACGCAACTTCGA+TGG 0.570994 4_4:+52501340 None:intergenic
GGGTCCAGAGGGTTTGAAGG+TGG 0.574431 4_4:+52502406 None:intergenic
TCCAGAGGGTTTGAAGGTGG+AGG 0.577332 4_4:+52502409 None:intergenic
AATTGATTGAAAGAGTAATA+CGG 0.577716 4_4:+52502094 None:intergenic
TTGAGAGAAAGAGTTGCAAA+TGG 0.578919 4_4:-52501370 Msa0687600:CDS
AATGCGGCAGACCAGGTCAC+TGG 0.581408 4_4:-52502508 Msa0687600:CDS
CAACCTCATGGCCGCGACCG+TGG 0.581868 4_4:+52502261 None:intergenic
GAAGTCAACTATTCAATCAC+GGG 0.583779 4_4:+52499856 None:intergenic
GACGGCGGATCTGGGTCCAG+AGG 0.589738 4_4:+52502394 None:intergenic
CGTGGTATTTGAAGTTGCGA+GGG 0.591626 4_4:+52502279 None:intergenic
GAGGGTTTGAAGGTGGAGGT+AGG 0.595710 4_4:+52502413 None:intergenic
AACATCCCAGATGGTGAACA+AGG 0.595960 4_4:-52501307 Msa0687600:intron
ACGGCGGATCTGGGTCCAGA+GGG 0.600152 4_4:+52502395 None:intergenic
GAGCTTCTTCTCTCTGATGA+CGG 0.601771 4_4:-52502326 Msa0687600:CDS
GGTGGAGGTAGGTGGATCGG+CGG 0.604040 4_4:+52502424 None:intergenic
ATTGAATAGTTGACTTCTGG+TGG 0.606510 4_4:-52499850 Msa0687600:three_prime_UTR
ATACAGCTAAACCCAATGTA+GGG 0.609042 4_4:+52500753 None:intergenic
AGAAGTCAACTATTCAATCA+CGG 0.616603 4_4:+52499855 None:intergenic
AGAGAAAGAGTTGCAAATGG+CGG 0.622059 4_4:-52501367 Msa0687600:CDS
AGAAAGAGTTGCAAATGGCG+GGG 0.622135 4_4:-52501365 Msa0687600:CDS
GTTGCGTGAACCACCAGTTG+TGG 0.628493 4_4:-52501332 Msa0687600:CDS
ATACCACGGTCGCGGCCATG+AGG 0.648998 4_4:-52502264 Msa0687600:intron
GGGATGTTGTCCACAACTGG+TGG 0.651915 4_4:+52501322 None:intergenic
AGCTGTATCTGCAGAAAACG+GGG 0.656668 4_4:-52500738 Msa0687600:CDS
GATTTAGAGTAAACTAAACA+AGG 0.664144 4_4:+52499878 None:intergenic
GTTGTGGACAACATCCCAGA+TGG 0.677611 4_4:-52501316 Msa0687600:CDS
ACATTCGAGACAGAGAGAGA+AGG 0.679568 4_4:-52502582 Msa0687600:five_prime_UTR
CCTCGCAACTTCAAATACCA+CGG 0.686072 4_4:-52502278 Msa0687600:CDS
GCAACAGGTTGTTACAAATG+CGG 0.691238 4_4:-52502524 Msa0687600:CDS
AATAGCGAAGAACGTAACCA+AGG 0.694872 4_4:+52502303 None:intergenic
TATCAAAGCTAGGAAGACTG+TGG 0.695853 4_4:+52499940 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAACAATTAATTAAGAAAA+AGG + chr4_4:52500122-52500141 None:intergenic 10.0%
!! AATATAAATAAAACTCACTT+CGG + chr4_4:52501731-52501750 None:intergenic 15.0%
!!! ACTTAATTGTTTGTTTTGTT+AGG - chr4_4:52500171-52500190 Msa0687600:intron 20.0%
!! AATTGATTGAAAGAGTAATA+CGG + chr4_4:52500253-52500272 None:intergenic 20.0%
!!! TTCAATTACACTCAAAATTT+TGG + chr4_4:52500404-52500423 None:intergenic 20.0%
!!! TTTTTAAGCTTCGTTATTAT+GGG + chr4_4:52500470-52500489 None:intergenic 20.0%
!!! ATTTTTAAGCTTCGTTATTA+TGG + chr4_4:52500471-52500490 None:intergenic 20.0%
!! AATAACGAAGCTTAAAAATA+AGG - chr4_4:52500472-52500491 Msa0687600:intron 20.0%
!!! ATGCATATTTTTCTTTCAAT+TGG - chr4_4:52500732-52500751 Msa0687600:CDS 20.0%
!! TGAAAAAAGGAAAATTAAGA+GGG + chr4_4:52501305-52501324 None:intergenic 20.0%
!! TTGAAAAAAGGAAAATTAAG+AGG + chr4_4:52501306-52501325 None:intergenic 20.0%
!!! GAAATTTGTTAAATCACTTT+TGG - chr4_4:52501833-52501852 Msa0687600:intron 20.0%
!!! TCATTTTCTGAGTTTAAATA+TGG - chr4_4:52502608-52502627 Msa0687600:five_prime_UTR 20.0%
! AACAAACAATTAAGTGATTG+CGG + chr4_4:52500166-52500185 None:intergenic 25.0%
! AGATGAGTACAAATATGATT+AGG - chr4_4:52500423-52500442 Msa0687600:intron 25.0%
! GATGAGTACAAATATGATTA+GGG - chr4_4:52500424-52500443 Msa0687600:intron 25.0%
! ATGAGTACAAATATGATTAG+GGG - chr4_4:52500425-52500444 Msa0687600:intron 25.0%
! AAGCTTAAAAATAAGGACTT+TGG - chr4_4:52500479-52500498 Msa0687600:intron 25.0%
! TGATTGATGGAAAATAGTTA+TGG + chr4_4:52500702-52500721 None:intergenic 25.0%
! TAAAAAAATAGCAGAGGAAT+CGG + chr4_4:52500786-52500805 None:intergenic 25.0%
! AAAGCATAAAAAAATAGCAG+AGG + chr4_4:52500792-52500811 None:intergenic 25.0%
! TCAACATATATAAAGCCATA+GGG + chr4_4:52500860-52500879 None:intergenic 25.0%
! TAAAACACAAAGTTTGAACA+AGG + chr4_4:52500912-52500931 None:intergenic 25.0%
! ACAATTATTAATTCAAACGC+AGG + chr4_4:52501119-52501138 None:intergenic 25.0%
!! TAATTGTTTTCTTAAATCCG+TGG - chr4_4:52501132-52501151 Msa0687600:intron 25.0%
!!! TTGAGTTGATAATTGTTTCT+AGG + chr4_4:52501196-52501215 None:intergenic 25.0%
! CAGGTGATTAATTCAATAAA+TGG + chr4_4:52501242-52501261 None:intergenic 25.0%
! ATTCGCATACAATTGAAAAA+AGG + chr4_4:52501318-52501337 None:intergenic 25.0%
! TTGATCAAATTGTCAATTGT+TGG + chr4_4:52501372-52501391 None:intergenic 25.0%
!! ATGAAGGAATACATAGATTT+TGG - chr4_4:52501424-52501443 Msa0687600:intron 25.0%
!! AACTGATTCATATTTCAAAC+AGG - chr4_4:52501456-52501475 Msa0687600:intron 25.0%
!! ACTGATTCATATTTCAAACA+GGG - chr4_4:52501457-52501476 Msa0687600:intron 25.0%
! ATTTCCTTTCACAAAAGTAA+AGG + chr4_4:52501818-52501837 None:intergenic 25.0%
! GTTTATCACTAAATAGATGT+GGG + chr4_4:52502124-52502143 None:intergenic 25.0%
! TTACAATGAATATCAAAGCT+AGG + chr4_4:52502417-52502436 None:intergenic 25.0%
!! GATTTAGAGTAAACTAAACA+AGG + chr4_4:52502469-52502488 None:intergenic 25.0%
CTCGAATGTGTATTGAAAAT+GGG + chr4_4:52499751-52499770 None:intergenic 30.0%
TCTCGAATGTGTATTGAAAA+TGG + chr4_4:52499752-52499771 None:intergenic 30.0%
TGAGATTAATTACAACCTCA+TGG + chr4_4:52500098-52500117 None:intergenic 30.0%
! TTTTGTTAGGTTCGTGATTT+GGG - chr4_4:52500184-52500203 Msa0687600:intron 30.0%
GCATAATGTTAAAAGTTCAG+TGG - chr4_4:52500363-52500382 Msa0687600:intron 30.0%
CATTAGTTGAAGATGATTGA+TGG + chr4_4:52500715-52500734 None:intergenic 30.0%
CTCAACATATATAAAGCCAT+AGG + chr4_4:52500861-52500880 None:intergenic 30.0%
ACAACATCCTATAGTAAAGT+TGG - chr4_4:52501170-52501189 Msa0687600:intron 30.0%
AAAAGGAAAATTAAGAGGGA+AGG + chr4_4:52501301-52501320 None:intergenic 30.0%
CAATATTCCAGTGAACTATA+TGG + chr4_4:52501496-52501515 None:intergenic 30.0%
AACAAATAGATCTTCTTACG+TGG + chr4_4:52501773-52501792 None:intergenic 30.0%
!! TGAACCTTTACTTTTGTGAA+AGG - chr4_4:52501811-52501830 Msa0687600:intron 30.0%
TACTCACAAATTTGTGAGAA+TGG + chr4_4:52501880-52501899 None:intergenic 30.0%
TAATAACACACTTCTGGATA+AGG + chr4_4:52501949-52501968 None:intergenic 30.0%
CATCATTAATAACACACTTC+TGG + chr4_4:52501955-52501974 None:intergenic 30.0%
! AGTGTGTTATTAATGATGCA+AGG - chr4_4:52501957-52501976 Msa0687600:intron 30.0%
! GTGTGTTATTAATGATGCAA+GGG - chr4_4:52501958-52501977 Msa0687600:intron 30.0%
AGAAATCTAGAGACTTAAGA+AGG + chr4_4:52502062-52502081 None:intergenic 30.0%
GGTTTATCACTAAATAGATG+TGG + chr4_4:52502125-52502144 None:intergenic 30.0%
GATAAACCAAATCGTAAACA+AGG - chr4_4:52502137-52502156 Msa0687600:CDS 30.0%
ATCTGATGCAAAGTTTGTAA+CGG - chr4_4:52502160-52502179 Msa0687600:intron 30.0%
! ATTACCTTCTCTTTTATTGC+AGG - chr4_4:52502184-52502203 Msa0687600:intron 30.0%
TCAAATGAAGTCCCAAATAA+TGG - chr4_4:52502278-52502297 Msa0687600:CDS 30.0%
AGAAGTCAACTATTCAATCA+CGG + chr4_4:52502492-52502511 None:intergenic 30.0%
TACATTGCAATCTTGAAGAT+TGG - chr4_4:52502544-52502563 Msa0687600:CDS 30.0%
TGTTTCGAAAGTAAATTGGT+TGG - chr4_4:52502569-52502588 Msa0687600:five_prime_UTR 30.0%
AGAAGAGAAGAGAAAATGTC+AGG - chr4_4:52499787-52499806 Msa0687600:three_prime_UTR 35.0%
!! GTTTTGTTAGGTTCGTGATT+TGG - chr4_4:52500183-52500202 Msa0687600:intron 35.0%
TTAAGCTTCGTTATTATGGG+TGG + chr4_4:52500467-52500486 None:intergenic 35.0%
TTAGTCTCGTGTATGCAAAA+AGG - chr4_4:52500631-52500650 Msa0687600:intron 35.0%
CTCTTTCTCTCAATTCTCTA+AGG + chr4_4:52500967-52500986 None:intergenic 35.0%
TTGAGAGAAAGAGTTGCAAA+TGG - chr4_4:52500974-52500993 Msa0687600:intron 35.0%
GTTCTCTAAGTTTACAACCA+CGG + chr4_4:52501152-52501171 None:intergenic 35.0%
TTCTAGGCCAACTTTACTAT+AGG + chr4_4:52501180-52501199 None:intergenic 35.0%
TCACCTGTTATGAAGTTGTT+CGG - chr4_4:52501255-52501274 Msa0687600:intron 35.0%
TTTCCGAACAACTTCATAAC+AGG + chr4_4:52501261-52501280 None:intergenic 35.0%
!! ATTGTTGGTTCTAATCTCTC+TGG + chr4_4:52501357-52501376 None:intergenic 35.0%
ATTTGATCAATGAAGCTCAC+TGG - chr4_4:52501382-52501401 Msa0687600:CDS 35.0%
! TCATTTTGTCTACCTATGGT+TGG + chr4_4:52501408-52501427 None:intergenic 35.0%
ACCATAGGTAGACAAAATGA+AGG - chr4_4:52501408-52501427 Msa0687600:intron 35.0%
! TCCTTCATTTTGTCTACCTA+TGG + chr4_4:52501412-52501431 None:intergenic 35.0%
GTGAACTATATGGAATCAGT+TGG + chr4_4:52501486-52501505 None:intergenic 35.0%
! ACTGATTCCATATAGTTCAC+TGG - chr4_4:52501486-52501505 Msa0687600:intron 35.0%
TTTGCTAAGTATCCCTACAT+TGG - chr4_4:52501579-52501598 Msa0687600:intron 35.0%
TTGCTAAGTATCCCTACATT+GGG - chr4_4:52501580-52501599 Msa0687600:intron 35.0%
ATACAGCTAAACCCAATGTA+GGG + chr4_4:52501594-52501613 None:intergenic 35.0%
TTAGCTGTATCTGCAGAAAA+CGG - chr4_4:52501604-52501623 Msa0687600:intron 35.0%
!! ACATGCTGACTTCATAACAA+AGG + chr4_4:52502095-52502114 None:intergenic 35.0%
! TCAGATCCTTGTTTACGATT+TGG + chr4_4:52502146-52502165 None:intergenic 35.0%
! TTTTATTGCAGGAACCTTCT+CGG - chr4_4:52502195-52502214 Msa0687600:intron 35.0%
! ACAGAAATCACATTTTGCAC+GGG + chr4_4:52502221-52502240 None:intergenic 35.0%
! CACAGAAATCACATTTTGCA+CGG + chr4_4:52502222-52502241 None:intergenic 35.0%
!!! TTCTCAGTTGTGCTACTTTT+GGG + chr4_4:52502254-52502273 None:intergenic 35.0%
! ACAGACTGGTTCCATTATTT+GGG + chr4_4:52502292-52502311 None:intergenic 35.0%
TCAACCTATTTGCTTCCATA+CGG + chr4_4:52502349-52502368 None:intergenic 35.0%
! TATGGAAGCAAATAGGTTGA+AGG - chr4_4:52502349-52502368 Msa0687600:CDS 35.0%
GAAGTCAACTATTCAATCAC+GGG + chr4_4:52502491-52502510 None:intergenic 35.0%
! GTGATTGAATAGTTGACTTC+TGG - chr4_4:52502491-52502510 Msa0687600:CDS 35.0%
! ATTGAATAGTTGACTTCTGG+TGG - chr4_4:52502494-52502513 Msa0687600:CDS 35.0%
GGCATGTTTCGAAAGTAAAT+TGG - chr4_4:52502565-52502584 Msa0687600:five_prime_UTR 35.0%
GAAGAGAAAATGTCAGGATC+CGG - chr4_4:52499793-52499812 Msa0687600:three_prime_UTR 40.0%
GCAACAGGTTGTTACAAATG+CGG - chr4_4:52499820-52499839 Msa0687600:three_prime_UTR 40.0%
! TAGGGTTTGGATTGGGATTT+GGG + chr4_4:52499885-52499904 None:intergenic 40.0%
! TTAGGGTTTGGATTGGGATT+TGG + chr4_4:52499886-52499905 None:intergenic 40.0%
AATAGCGAAGAACGTAACCA+AGG + chr4_4:52500044-52500063 None:intergenic 40.0%
TGCTTGACTTATACTCTGAC+TGG - chr4_4:52500212-52500231 Msa0687600:intron 40.0%
TTGAGAAAGTTGCTGCTACT+AGG - chr4_4:52500284-52500303 Msa0687600:intron 40.0%
!! AGTTCAGTGGATTAGTGTTC+AGG - chr4_4:52500376-52500395 Msa0687600:intron 40.0%
AATCCCTTCTAACGGCAATT+GGG + chr4_4:52500555-52500574 None:intergenic 40.0%
TAATCCCTTCTAACGGCAAT+TGG + chr4_4:52500556-52500575 None:intergenic 40.0%
CGACAGTTAATCCCTTCTAA+CGG + chr4_4:52500563-52500582 None:intergenic 40.0%
CGTTAGAAGGGATTAACTGT+CGG - chr4_4:52500561-52500580 Msa0687600:intron 40.0%
!! TCAATTCTCTAAGGCTAGTC+TGG + chr4_4:52500958-52500977 None:intergenic 40.0%
AGAGAAAGAGTTGCAAATGG+CGG - chr4_4:52500977-52500996 Msa0687600:intron 40.0%
GATTACCTTGTTCACCATCT+GGG + chr4_4:52501045-52501064 None:intergenic 40.0%
AGATTACCTTGTTCACCATC+TGG + chr4_4:52501046-52501065 None:intergenic 40.0%
GATACAGCTAAACCCAATGT+AGG + chr4_4:52501595-52501614 None:intergenic 40.0%
TAGCTGTATCTGCAGAAAAC+GGG - chr4_4:52501605-52501624 Msa0687600:intron 40.0%
! TCGACGTCGTTAACATTTTC+AGG + chr4_4:52501667-52501686 None:intergenic 40.0%
GGTTCCTGCAATAAAAGAGA+AGG + chr4_4:52502191-52502210 None:intergenic 40.0%
!!! CTTCTCAGTTGTGCTACTTT+TGG + chr4_4:52502255-52502274 None:intergenic 40.0%
! GACAGACTGGTTCCATTATT+TGG + chr4_4:52502293-52502312 None:intergenic 40.0%
CAACCTATTTGCTTCCATAC+GGG + chr4_4:52502348-52502367 None:intergenic 40.0%
!! ATAGGTTGAAGGCACTAGAA+AGG - chr4_4:52502360-52502379 Msa0687600:CDS 40.0%
TATCAAAGCTAGGAAGACTG+TGG + chr4_4:52502407-52502426 None:intergenic 40.0%
ACATTCGAGACAGAGAGAGA+AGG - chr4_4:52499762-52499781 Msa0687600:three_prime_UTR 45.0%
! GTAACAACCTGTTGCTGTTC+CGG + chr4_4:52499815-52499834 None:intergenic 45.0%
! AGGGTTTGGATTGGGATTTG+GGG + chr4_4:52499884-52499903 None:intergenic 45.0%
! GTGGTGTTAGGGTTTGGATT+GGG + chr4_4:52499892-52499911 None:intergenic 45.0%
!! AGGCTTTTTGGGCTTTTCGA+CGG + chr4_4:52499971-52499990 None:intergenic 45.0%
TCATCAGAGAGAAGAAGCTC+GGG + chr4_4:52500018-52500037 None:intergenic 45.0%
GTCATCAGAGAGAAGAAGCT+CGG + chr4_4:52500019-52500038 None:intergenic 45.0%
GAGCTTCTTCTCTCTGATGA+CGG - chr4_4:52500018-52500037 Msa0687600:CDS 45.0%
! CGTGGTATTTGAAGTTGCGA+GGG + chr4_4:52500068-52500087 None:intergenic 45.0%
CCTCGCAACTTCAAATACCA+CGG - chr4_4:52500066-52500085 Msa0687600:CDS 45.0%
AACTTCAAATACCACGGTCG+CGG - chr4_4:52500072-52500091 Msa0687600:CDS 45.0%
TTTACGCGTGAGTCAACTCT+AGG - chr4_4:52500515-52500534 Msa0687600:intron 45.0%
ATCCCTTCTAACGGCAATTG+GGG + chr4_4:52500554-52500573 None:intergenic 45.0%
!! TCTAAGGCTAGTCTGGTTAG+AGG + chr4_4:52500951-52500970 None:intergenic 45.0%
GAGAAAGAGTTGCAAATGGC+GGG - chr4_4:52500978-52500997 Msa0687600:intron 45.0%
AGAAAGAGTTGCAAATGGCG+GGG - chr4_4:52500979-52500998 Msa0687600:intron 45.0%
AACATCCCAGATGGTGAACA+AGG - chr4_4:52501037-52501056 Msa0687600:intron 45.0%
!! GAGGGAAGGTGTTCTAGTTA+AGG + chr4_4:52501287-52501306 None:intergenic 45.0%
AGCTGTATCTGCAGAAAACG+GGG - chr4_4:52501606-52501625 Msa0687600:intron 45.0%
TTAGAGACTACTCGAACGTC+TGG - chr4_4:52502016-52502035 Msa0687600:intron 45.0%
! ATAGTTGACTTCTGGTGGTC+TGG - chr4_4:52502499-52502518 Msa0687600:CDS 45.0%
TGTTACAAATGCGGCAGACC+AGG - chr4_4:52499829-52499848 Msa0687600:three_prime_UTR 50.0%
! GTTTGGATTGGGATTTGGGG+CGG + chr4_4:52499881-52499900 None:intergenic 50.0%
! GGTGGTGTTAGGGTTTGGAT+TGG + chr4_4:52499893-52499912 None:intergenic 50.0%
!! CTTTTTGGGCTTTTCGACGG+CGG + chr4_4:52499968-52499987 None:intergenic 50.0%
! AGAAGCTCGGGAGTGAGTTT+TGG + chr4_4:52500006-52500025 None:intergenic 50.0%
! CCGTGGTATTTGAAGTTGCG+AGG + chr4_4:52500069-52500088 None:intergenic 50.0%
TGCTGCTACTAGGCGTGTTA+AGG - chr4_4:52500294-52500313 Msa0687600:intron 50.0%
TTAGGGGCCTGACAGTTACT+TGG - chr4_4:52500441-52500460 Msa0687600:intron 50.0%
CTAACGGCAATTGGGGACAA+TGG + chr4_4:52500547-52500566 None:intergenic 50.0%
TGTCCCCAATTGCCGTTAGA+AGG - chr4_4:52500548-52500567 Msa0687600:intron 50.0%
GTCCCCAATTGCCGTTAGAA+GGG - chr4_4:52500549-52500568 Msa0687600:intron 50.0%
GGGAGAGAGATACAGAGCAA+TGG + chr4_4:52500840-52500859 None:intergenic 50.0%
GCTCTGTATCTCTCTCCCTA+TGG - chr4_4:52500842-52500861 Msa0687600:intron 50.0%
! TCTGGGATGTTGTCCACAAC+TGG + chr4_4:52501028-52501047 None:intergenic 50.0%
GTTGTGGACAACATCCCAGA+TGG - chr4_4:52501028-52501047 Msa0687600:intron 50.0%
TGCTGTTATCTCAGCACTGC+TGG + chr4_4:52502315-52502334 None:intergenic 50.0%
GAGCCCGTATGGAAGCAAAT+AGG - chr4_4:52502342-52502361 Msa0687600:CDS 50.0%
!! AGACTGTGGTATGCTGGCTT+TGG + chr4_4:52502393-52502412 None:intergenic 50.0%
CTAGGAAGACTGTGGTATGC+TGG + chr4_4:52502399-52502418 None:intergenic 50.0%
TCAGGATCCGGAACAGCAAC+AGG - chr4_4:52499805-52499824 Msa0687600:three_prime_UTR 55.0%
! TTGGGATTTGGGGCGGTGAA+AGG + chr4_4:52499874-52499893 None:intergenic 55.0%
! GAGGTGGTGGTGTTAGGGTT+TGG + chr4_4:52499898-52499917 None:intergenic 55.0%
GAAGGTGGAGGTAGGTGGAT+CGG + chr4_4:52499926-52499945 None:intergenic 55.0%
GGTTTGAAGGTGGAGGTAGG+TGG + chr4_4:52499931-52499950 None:intergenic 55.0%
GAGGGTTTGAAGGTGGAGGT+AGG + chr4_4:52499934-52499953 None:intergenic 55.0%
ACCTCCACCTTCAAACCCTC+TGG - chr4_4:52499934-52499953 Msa0687600:exon 55.0%
TCCAGAGGGTTTGAAGGTGG+AGG + chr4_4:52499938-52499957 None:intergenic 55.0%
TCTGGGTCCAGAGGGTTTGA+AGG + chr4_4:52499944-52499963 None:intergenic 55.0%
! CGGGTACGTGGAGGCTTTTT+GGG + chr4_4:52499982-52500001 None:intergenic 55.0%
! ACGGGTACGTGGAGGCTTTT+TGG + chr4_4:52499983-52500002 None:intergenic 55.0%
!!! AGTTTTGGACGGGTACGTGG+AGG + chr4_4:52499991-52500010 None:intergenic 55.0%
!!! GTGAGTTTTGGACGGGTACG+TGG + chr4_4:52499994-52500013 None:intergenic 55.0%
!!! CTCGGGAGTGAGTTTTGGAC+GGG + chr4_4:52500001-52500020 None:intergenic 55.0%
!!! GCTCGGGAGTGAGTTTTGGA+CGG + chr4_4:52500002-52500021 None:intergenic 55.0%
!! CTTCTCTCTGATGACGGCCT+TGG - chr4_4:52500024-52500043 Msa0687600:CDS 55.0%
TGGGTGGCCAAGTAACTGTC+AGG + chr4_4:52500451-52500470 None:intergenic 55.0%
ACGGCAATTGGGGACAATGG+CGG + chr4_4:52500544-52500563 None:intergenic 55.0%
GGTGGTTCACGCAACTTCGA+TGG + chr4_4:52501007-52501026 None:intergenic 55.0%
GTTGCGTGAACCACCAGTTG+TGG - chr4_4:52501012-52501031 Msa0687600:intron 55.0%
GGGATGTTGTCCACAACTGG+TGG + chr4_4:52501025-52501044 None:intergenic 55.0%
GAAGCTCACTGGCCAACCAT+AGG - chr4_4:52501393-52501412 Msa0687600:intron 55.0%
! ACATTTTGCACGGGCCGAGA+AGG + chr4_4:52502212-52502231 None:intergenic 55.0%
AATGCGGCAGACCAGGTCAC+TGG - chr4_4:52499836-52499855 Msa0687600:three_prime_UTR 60.0%
! CGGTGGAGGTGGTGGTGTTA+GGG + chr4_4:52499903-52499922 None:intergenic 60.0%
GGGTCCAGAGGGTTTGAAGG+TGG + chr4_4:52499941-52499960 None:intergenic 60.0%
! GGCTTTTCGACGGCGGATCT+GGG + chr4_4:52499961-52499980 None:intergenic 60.0%
CTCAGCACTGCTGGACAGAC+TGG + chr4_4:52502306-52502325 None:intergenic 60.0%
AGCAGAGCAGAGAGCCCGTA+TGG - chr4_4:52502331-52502350 Msa0687600:CDS 60.0%
CAGTCGCGTGACCAGTGACC+TGG + chr4_4:52499850-52499869 None:intergenic 65.0%
! GCGGTGGAGGTGGTGGTGTT+AGG + chr4_4:52499904-52499923 None:intergenic 65.0%
GGTGGAGGTAGGTGGATCGG+CGG + chr4_4:52499923-52499942 None:intergenic 65.0%
ACGGCGGATCTGGGTCCAGA+GGG + chr4_4:52499952-52499971 None:intergenic 65.0%
! GGGCTTTTCGACGGCGGATC+TGG + chr4_4:52499962-52499981 None:intergenic 65.0%
ATACCACGGTCGCGGCCATG+AGG - chr4_4:52500080-52500099 Msa0687600:CDS 65.0%
TGGATCGGCGGTGGAGGTGG+TGG + chr4_4:52499911-52499930 None:intergenic 70.0%
AGGTGGATCGGCGGTGGAGG+TGG + chr4_4:52499914-52499933 None:intergenic 70.0%
GGTAGGTGGATCGGCGGTGG+AGG + chr4_4:52499917-52499936 None:intergenic 70.0%
GGAGGTAGGTGGATCGGCGG+TGG + chr4_4:52499920-52499939 None:intergenic 70.0%
GACGGCGGATCTGGGTCCAG+AGG + chr4_4:52499953-52499972 None:intergenic 70.0%
CAACCTCATGGCCGCGACCG+TGG + chr4_4:52500086-52500105 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 52499729 52502637 52499729 ID=Msa0687600;Name=Msa0687600
chr4_4 mRNA 52499729 52502637 52499729 ID=Msa0687600-mRNA-1;Parent=Msa0687600;Name=Msa0687600-mRNA-1;_AED=0.01;_eAED=0.01;_QI=73|0.75|0.8|1|1|1|5|219|297
chr4_4 exon 52499729 52500160 52499729 ID=Msa0687600-mRNA-1:exon:13839;Parent=Msa0687600-mRNA-1
chr4_4 exon 52500634 52500791 52500634 ID=Msa0687600-mRNA-1:exon:13838;Parent=Msa0687600-mRNA-1
chr4_4 exon 52501308 52501407 52501308 ID=Msa0687600-mRNA-1:exon:13837;Parent=Msa0687600-mRNA-1
chr4_4 exon 52502051 52502173 52502051 ID=Msa0687600-mRNA-1:exon:13836;Parent=Msa0687600-mRNA-1
chr4_4 exon 52502265 52502637 52502265 ID=Msa0687600-mRNA-1:exon:13835;Parent=Msa0687600-mRNA-1
chr4_4 five_prime_UTR 52502565 52502637 52502565 ID=Msa0687600-mRNA-1:five_prime_utr;Parent=Msa0687600-mRNA-1
chr4_4 CDS 52502265 52502564 52502265 ID=Msa0687600-mRNA-1:cds;Parent=Msa0687600-mRNA-1
chr4_4 CDS 52502051 52502173 52502051 ID=Msa0687600-mRNA-1:cds;Parent=Msa0687600-mRNA-1
chr4_4 CDS 52501308 52501407 52501308 ID=Msa0687600-mRNA-1:cds;Parent=Msa0687600-mRNA-1
chr4_4 CDS 52500634 52500791 52500634 ID=Msa0687600-mRNA-1:cds;Parent=Msa0687600-mRNA-1
chr4_4 CDS 52499948 52500160 52499948 ID=Msa0687600-mRNA-1:cds;Parent=Msa0687600-mRNA-1
chr4_4 three_prime_UTR 52499729 52499947 52499729 ID=Msa0687600-mRNA-1:three_prime_utr;Parent=Msa0687600-mRNA-1
Gene Sequence

>Msa0687600

ATGTCAGGATCCGGAACAGCAACAGGTTGTTACAAATGCGGCAGACCAGGTCACTGGTCACGCGACTGTCCTTTCACCGCCCCAAATCCCAATCCAAACCCTAACACCACCACCTCCACCGCCGATCCACCTACCTCCACCTTCAAACCCTCTGGACCCAGATCCGCCGTCGAAAAGCCCAAAAAGCCTCCACGTACCCGTCCAAAACTCACTCCCGAGCTTCTTCTCTCTGATGACGGCCTTGGTTACGTTCTTCGCTATTTCCCTCGCAACTTCAAATACCACGGTCGCGGCCATGAGGTTCGTGATTTGGGTAAGTTGCTTGACTTATACTCTGACTGGCACTCTCGTTTGCTACCGTATTACTCTTTCAATCAATTTGTCAATAAAGTTGAGAAAGTTGCTGCTACTAGGCGTGTTAAGACTAGCCTTAGAGAATTGAGAGAAAGAGTTGCAAATGGCGGGGATCCATCGAAGTTGCGTGAACCACCAGTTGTGGACAACATCCCAGATGGTGAACAAGTATCTTTGCTAAGTATCCCTACATTGGGTTTAGCTGTATCTGCAGAAAACGGGGAAGCAAGTCATCAAGATAATGAGATGTTTGCAGAACCTGAAAATGTTAACGACGTCGACATTCAAGAAGATTTGTTCAATGATATATATGATAAAGCTACCGAAGAACCTTCTCGGCCCGTGCAAAATGTGATTTCTGTGAGTACTGATCCCAAAAGTAGCACAACTGAGAAGACATCAAATGAAGTCCCAAATAATGGAACCAGTCTGTCCAGCAGTGCTGAGATAACAGCAGAGCAGAGAGCCCGTATGGAAGCAAATAGGTTGAAGGCACTAGAAAGGCGTGCAGCCAAAGCCAGCATACCACAGTCTTCCTAG
Protein sequence

>Msa0687600

MSGSGTATGCYKCGRPGHWSRDCPFTAPNPNPNPNTTTSTADPPTSTFKPSGPRSAVEKPKKPPRTRPKLTPELLLSDDGLGYVLRYFPRNFKYHGRGHEVRDLGKLLDLYSDWHSRLLPYYSFNQFVNKVEKVAATRRVKTSLRELRERVANGGDPSKLREPPVVDNIPDGEQVSLLSIPTLGLAVSAENGEASHQDNEMFAEPENVNDVDIQEDLFNDIYDKATEEPSRPVQNVISVSTDPKSSTTEKTSNEVPNNGTSLSSSAEITAEQRARMEANRLKALERRAAKASIPQSS*