AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0689770

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0689770 MtrunA17_Chr5g0404041 87.500 48 6 0 1 48 1 48 3.66e-25 87.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0689770 AT3G50845.1 65.789 38 13 0 1 38 1 38 2.02e-11 55.5

Find 52 sgRNAs with CRISPR-Local

Find 204 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTTCTGTTGATGACAAAT+TGG 0.159295 4_4:+56989495 Msa0689770:three_prime_UTR
CAAATTTCGTGAGAGATATT+AGG 0.180944 4_4:+56989419 Msa0689770:three_prime_UTR
AATGCCTTCACAATAGTTAT+TGG 0.230337 4_4:-56992623 None:intergenic
TCATTATGTCTTTAGTTCTT+TGG 0.260015 4_4:+56992674 Msa0689770:three_prime_UTR
AGTTTGCATCATTTCGCTTT+TGG 0.271870 4_4:-56989088 None:intergenic
CTTTAGTTCTTTGGTTTCCA+TGG 0.295092 4_4:+56992683 Msa0689770:three_prime_UTR
TTAAGCTGTTCCAATGAATA+AGG 0.330883 4_4:-56989454 None:intergenic
TATGTTTGTTCACAGTTCAT+TGG 0.347534 4_4:-56992588 None:intergenic
AACTTTACCTGATTCTTCAT+TGG 0.354188 4_4:-56992286 None:intergenic
AAATTTCGTGAGAGATATTA+GGG 0.354298 4_4:+56989420 Msa0689770:three_prime_UTR
TCCAATGAATAAGGATGTTC+AGG 0.357418 4_4:-56989445 None:intergenic
TCCTGAACATCCTTATTCAT+TGG 0.366089 4_4:+56989444 Msa0689770:three_prime_UTR
ATGCCTTCACAATAGTTATT+GGG 0.370934 4_4:-56992622 None:intergenic
TCCACAAGCTGGCGCAGGTT+TGG 0.379145 4_4:-56992551 None:intergenic
CAATTTGTCATCAACAGAAA+TGG 0.383548 4_4:-56989494 None:intergenic
GTGGGGTTGGTTGGGAAGAA+AGG 0.391030 4_4:-56992718 None:intergenic
ATTTCTGTTGATGACAAATT+GGG 0.401971 4_4:+56989496 Msa0689770:three_prime_UTR
GCGCAGGTTTGGATTCTCTA+AGG 0.406934 4_4:-56992540 None:intergenic
TAACATGAGCTTGGAGTATG+AGG 0.410865 4_4:-56992863 None:intergenic
TGGGGTTGGTTGGGAAGAAA+GGG 0.413412 4_4:-56992717 None:intergenic
TTTAGTTCTTTGGTTTCCAT+GGG 0.424840 4_4:+56992684 Msa0689770:three_prime_UTR
TCTCATGCCAATGAAGAATC+AGG 0.430582 4_4:+56992279 Msa0689770:three_prime_UTR
ATGATCAGAGTGAGGTTCTT+CGG 0.436626 4_4:-56989266 None:intergenic
GACGGTTCGACACTGTAGTA+TGG 0.437697 4_4:+56989628 Msa0689770:three_prime_UTR
GTCTTGAAAGCGATGACACT+TGG 0.447355 4_4:+56989190 Msa0689770:exon
AACATGATGTGGGGTTGGTT+GGG 0.451572 4_4:-56992726 None:intergenic
TCCAAACCTGCGCCAGCTTG+TGG 0.483104 4_4:+56992550 Msa0689770:three_prime_UTR
TGTAGTATGGTTACTGTGAT+TGG 0.485275 4_4:+56989641 Msa0689770:three_prime_UTR
AAACATGATGTGGGGTTGGT+TGG 0.497453 4_4:-56992727 None:intergenic
GGGGTTGGTTGGGAAGAAAG+GGG 0.509817 4_4:-56992716 None:intergenic
CTTCACAATAGTTATTGGGC+AGG 0.517991 4_4:-56992618 None:intergenic
TATTTGATGATTGATGATGA+TGG 0.518913 4_4:-56989131 None:intergenic
GTCAAATCAAATCAAAGTCA+AGG 0.520569 4_4:-56989397 None:intergenic
CCCCACATCATGTTTAGCAT+GGG 0.522342 4_4:+56992735 Msa0689770:three_prime_UTR
ACCCCACATCATGTTTAGCA+TGG 0.552675 4_4:+56992734 Msa0689770:three_prime_UTR
CCCCATGCTAAACATGATGT+GGG 0.557277 4_4:-56992736 None:intergenic
CTGTTGATGACAAATTGGGT+CGG 0.557673 4_4:+56989500 Msa0689770:three_prime_UTR
TGCTAAACATGATGTGGGGT+TGG 0.576444 4_4:-56992731 None:intergenic
TGAGCTTGGAGTATGAGGGC+TGG 0.583758 4_4:-56992858 None:intergenic
ATACTACAGTGTCGAACCGT+CGG 0.586741 4_4:-56989626 None:intergenic
CGAGACACTCATCCACAAGC+TGG 0.593597 4_4:-56992562 None:intergenic
CTGCCCAATAACTATTGTGA+AGG 0.600420 4_4:+56992619 Msa0689770:three_prime_UTR
ACTCATCCACAAGCTGGCGC+AGG 0.607822 4_4:-56992556 None:intergenic
GAGTGAGGTTCTTCGGAGCG+AGG 0.620124 4_4:-56989259 None:intergenic
GTGGACATCAAAGTATCTGA+AGG 0.621459 4_4:+56992255 Msa0689770:three_prime_UTR
CCCACATCATGTTTAGCATG+GGG 0.632974 4_4:+56992736 Msa0689770:three_prime_UTR
GTCATCGCTTTCAAGACTGA+CGG 0.633845 4_4:-56989184 None:intergenic
AACATGAGCTTGGAGTATGA+GGG 0.637702 4_4:-56992862 None:intergenic
AGGATATCATAAATATCGAG+AGG 0.652332 4_4:-56989298 None:intergenic
TCAACGAAATGATCAGAGTG+AGG 0.689074 4_4:-56989274 None:intergenic
CCCATGCTAAACATGATGTG+GGG 0.709124 4_4:-56992735 None:intergenic
CCACATCATGTTTAGCATGG+GGG 0.714707 4_4:+56992737 Msa0689770:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAAAAAAAATGAAAGA+AGG - chr4_4:56989321-56989340 None:intergenic 10.0%
!! AATTTGAATTGAATTTATGT+TGG + chr4_4:56989575-56989594 Msa0689770:intron 15.0%
!! AAAAAAAAACAAAGAAGAAA+GGG - chr4_4:56992359-56992378 None:intergenic 15.0%
!! AAAAAAAAAACAAAGAAGAA+AGG - chr4_4:56992360-56992379 None:intergenic 15.0%
!!! TAAAATGTTAAAGTTTGTTT+GGG - chr4_4:56992431-56992450 None:intergenic 15.0%
!!! ATAAAATGTTAAAGTTTGTT+TGG - chr4_4:56992432-56992451 None:intergenic 15.0%
!!! TTCTAGTTATAAGTATGTTT+TGG + chr4_4:56989831-56989850 Msa0689770:intron 20.0%
!!! TTCATCTTCAACTTATTTTT+AGG + chr4_4:56990250-56990269 Msa0689770:intron 20.0%
!!! AACTTAAGCATTTTTAAAGT+AGG + chr4_4:56991116-56991135 Msa0689770:intron 20.0%
!! ATTCAAATTTACACAACATT+TGG - chr4_4:56991183-56991202 None:intergenic 20.0%
!!! AACCTTAGTAATCTAAAATT+TGG - chr4_4:56991373-56991392 None:intergenic 20.0%
!!! ATTAGTTTATTATTCAGAGA+AGG + chr4_4:56991514-56991533 Msa0689770:intron 20.0%
!! TTAGTTTATTATTCAGAGAA+GGG + chr4_4:56991515-56991534 Msa0689770:intron 20.0%
!! TTGACTATAATTATGACTTT+GGG + chr4_4:56991801-56991820 Msa0689770:intron 20.0%
!! AAAAAAAACAAAGAAGAAAG+GGG - chr4_4:56992358-56992377 None:intergenic 20.0%
!!! TATTTGATGATTGATGATGA+TGG - chr4_4:56989134-56989153 None:intergenic 25.0%
! AAATTTCGTGAGAGATATTA+GGG + chr4_4:56989420-56989439 Msa0689770:three_prime_UTR 25.0%
!!! ATTTCTGTTGATGACAAATT+GGG + chr4_4:56989496-56989515 Msa0689770:three_prime_UTR 25.0%
!! TAATAACTTGTGAAGCAAAA+GGG - chr4_4:56989730-56989749 None:intergenic 25.0%
!! ATAATAACTTGTGAAGCAAA+AGG - chr4_4:56989731-56989750 None:intergenic 25.0%
! TATAGTGATTTATGTTTAGC+TGG + chr4_4:56989804-56989823 Msa0689770:intron 25.0%
!! GGCTGAAAATTAATTTTAGA+AGG + chr4_4:56989931-56989950 Msa0689770:intron 25.0%
!!! TGTATGTTTTGTTTCACTTT+TGG + chr4_4:56990028-56990047 Msa0689770:intron 25.0%
! TAAGTTAGAATCAATTCTGA+AGG - chr4_4:56990130-56990149 None:intergenic 25.0%
!!! ACAAAAATTGATTTTGTGTG+TGG - chr4_4:56990300-56990319 None:intergenic 25.0%
! TGAGTCAAAATTGATTATAG+AGG - chr4_4:56990631-56990650 None:intergenic 25.0%
!!! AATTTTGACTCATTCAAAAG+TGG + chr4_4:56990639-56990658 Msa0689770:intron 25.0%
! TAAATGTCCTATATGGAAAT+TGG + chr4_4:56990943-56990962 Msa0689770:intron 25.0%
!!! GAGAAATTTATTTTACGAAG+TGG + chr4_4:56991243-56991262 Msa0689770:intron 25.0%
!!! ATTTATTTTACGAAGTGGTT+AGG + chr4_4:56991248-56991267 Msa0689770:intron 25.0%
!! TGATTAATGAGCTTTAGTTA+AGG + chr4_4:56991277-56991296 Msa0689770:intron 25.0%
!! TAATCTAAAATTTGGCTAAG+AGG - chr4_4:56991365-56991384 None:intergenic 25.0%
!! AGCCAAATTTTAGATTACTA+AGG + chr4_4:56991368-56991387 Msa0689770:intron 25.0%
! ATTAAGAAGTGGAAACAAAA+GGG + chr4_4:56991473-56991492 Msa0689770:intron 25.0%
! TGTTACCTTGTATTTGTAAT+AGG + chr4_4:56991556-56991575 Msa0689770:intron 25.0%
! TTCAACCTATTACAAATACA+AGG - chr4_4:56991564-56991583 None:intergenic 25.0%
!! GTTTTCAAATTATGTTTCCA+CGG + chr4_4:56991769-56991788 Msa0689770:intron 25.0%
! CTTGACTATAATTATGACTT+TGG + chr4_4:56991800-56991819 Msa0689770:intron 25.0%
! ACTATATGATATAAGTAGTG+TGG - chr4_4:56992181-56992200 None:intergenic 25.0%
! TATAGTATAATTGTCTTCTC+TGG + chr4_4:56992195-56992214 Msa0689770:intron 25.0%
!!! TCATTATGTCTTTAGTTCTT+TGG + chr4_4:56992674-56992693 Msa0689770:three_prime_UTR 25.0%
!!! TAGGTGGTAAGTTTTAAAAA+TGG + chr4_4:56992893-56992912 Msa0689770:three_prime_UTR 25.0%
AGGATATCATAAATATCGAG+AGG - chr4_4:56989301-56989320 None:intergenic 30.0%
GTCAAATCAAATCAAAGTCA+AGG - chr4_4:56989400-56989419 None:intergenic 30.0%
CAAATTTCGTGAGAGATATT+AGG + chr4_4:56989419-56989438 Msa0689770:three_prime_UTR 30.0%
TTAAGCTGTTCCAATGAATA+AGG - chr4_4:56989457-56989476 None:intergenic 30.0%
CAATTTGTCATCAACAGAAA+TGG - chr4_4:56989497-56989516 None:intergenic 30.0%
!! CATTTCTGTTGATGACAAAT+TGG + chr4_4:56989495-56989514 Msa0689770:three_prime_UTR 30.0%
AAACAAACTTTGTAATGAGC+AGG - chr4_4:56989701-56989720 None:intergenic 30.0%
! GAAAAACTGATTCTAGCTAT+AGG + chr4_4:56989887-56989906 Msa0689770:intron 30.0%
! TGAGTTGAACACTAGATTAA+AGG + chr4_4:56989910-56989929 Msa0689770:intron 30.0%
CAACTTGAAGCTAAAATTTG+CGG - chr4_4:56989963-56989982 None:intergenic 30.0%
GTAGATAAATTTGACTCTAG+AGG + chr4_4:56990052-56990071 Msa0689770:intron 30.0%
ATTGTTCAACTAACCTTACA+TGG + chr4_4:56990206-56990225 Msa0689770:intron 30.0%
AAGATGAAGTAGTTTACACT+TGG - chr4_4:56990238-56990257 None:intergenic 30.0%
!! ATTTTGTGTGTGGATTGATT+TGG - chr4_4:56990290-56990309 None:intergenic 30.0%
! ATAAGTGTATTTCGTTTTGC+AGG + chr4_4:56990435-56990454 Msa0689770:intron 30.0%
! TAAGTGTATTTCGTTTTGCA+GGG + chr4_4:56990436-56990455 Msa0689770:intron 30.0%
TCTAACTTGAAGCTACAATT+TGG - chr4_4:56990578-56990597 None:intergenic 30.0%
CAAGTTAGAATCAATTCTGA+AGG + chr4_4:56990589-56990608 Msa0689770:intron 30.0%
! AGCTTTGGCTAAAAAATCAT+TGG + chr4_4:56990807-56990826 Msa0689770:intron 30.0%
GCTTTGGCTAAAAAATCATT+GGG + chr4_4:56990808-56990827 Msa0689770:intron 30.0%
CTTTGGCTAAAAAATCATTG+GGG + chr4_4:56990809-56990828 Msa0689770:intron 30.0%
! ACTTAATGGATGTGTATGTT+TGG - chr4_4:56990867-56990886 None:intergenic 30.0%
AAACATACACATCCATTAAG+TGG + chr4_4:56990866-56990885 Msa0689770:intron 30.0%
AAAGCATACAAACCACTTAA+TGG - chr4_4:56990881-56990900 None:intergenic 30.0%
ATAGGACATTTATGAGGTTA+TGG - chr4_4:56990935-56990954 None:intergenic 30.0%
AACCTCATAAATGTCCTATA+TGG + chr4_4:56990936-56990955 Msa0689770:intron 30.0%
TTCCATATAGGACATTTATG+AGG - chr4_4:56990941-56990960 None:intergenic 30.0%
GGATAAACCAATTTCCATAT+AGG - chr4_4:56990953-56990972 None:intergenic 30.0%
!!! TTTGGTTGTTTTGAAAGTCA+AGG - chr4_4:56990978-56990997 None:intergenic 30.0%
TTCAAAACAACCAAAACTTG+TGG + chr4_4:56990983-56991002 Msa0689770:intron 30.0%
!! TTCTCTTTCTTTTGAAAGAG+TGG + chr4_4:56991059-56991078 Msa0689770:intron 30.0%
! AGCTTTAGTTAAGGTTGAAT+TGG + chr4_4:56991286-56991305 Msa0689770:intron 30.0%
TTAGTTAAGGTTGAATTGGT+TGG + chr4_4:56991290-56991309 Msa0689770:intron 30.0%
TAGTTAAGGTTGAATTGGTT+GGG + chr4_4:56991291-56991310 Msa0689770:intron 30.0%
!! ATAGTAGAAGCATTAAGAAG+TGG + chr4_4:56991462-56991481 Msa0689770:intron 30.0%
CATTAAGAAGTGGAAACAAA+AGG + chr4_4:56991472-56991491 Msa0689770:intron 30.0%
TTAAGAAGTGGAAACAAAAG+GGG + chr4_4:56991474-56991493 Msa0689770:intron 30.0%
TAACTTCATTACAGTGTACA+CGG - chr4_4:56991843-56991862 None:intergenic 30.0%
ATATCAAACCATGTGAACAA+CGG - chr4_4:56992022-56992041 None:intergenic 30.0%
TTACTCAAATTCTTCTTTGC+AGG + chr4_4:56992233-56992252 Msa0689770:intron 30.0%
AACTTTACCTGATTCTTCAT+TGG - chr4_4:56992289-56992308 None:intergenic 30.0%
TGTTGAATGCAGATAAAGAA+GGG - chr4_4:56992385-56992404 None:intergenic 30.0%
! AGTTTGTTTGGGAATTTAGA+TGG - chr4_4:56992420-56992439 None:intergenic 30.0%
TATGTTTGTTCACAGTTCAT+TGG - chr4_4:56992591-56992610 None:intergenic 30.0%
ATGCCTTCACAATAGTTATT+GGG - chr4_4:56992625-56992644 None:intergenic 30.0%
AATGCCTTCACAATAGTTAT+TGG - chr4_4:56992626-56992645 None:intergenic 30.0%
!! TTTAGTTCTTTGGTTTCCAT+GGG + chr4_4:56992684-56992703 Msa0689770:three_prime_UTR 30.0%
!!! TCTGTATATTTTGCTGTTTG+TGG + chr4_4:56992829-56992848 Msa0689770:three_prime_UTR 30.0%
!!! CTGTATATTTTGCTGTTTGT+GGG + chr4_4:56992830-56992849 Msa0689770:three_prime_UTR 30.0%
TAAAAAGCTTAACATGAGCT+TGG - chr4_4:56992875-56992894 None:intergenic 30.0%
! AAGCTCATGTTAAGCTTTTT+AGG + chr4_4:56992874-56992893 Msa0689770:three_prime_UTR 30.0%
! AGTTTGCATCATTTCGCTTT+TGG - chr4_4:56989091-56989110 None:intergenic 35.0%
TCCTGAACATCCTTATTCAT+TGG + chr4_4:56989444-56989463 Msa0689770:three_prime_UTR 35.0%
TCCAATGAATAAGGATGTTC+AGG - chr4_4:56989448-56989467 None:intergenic 35.0%
!!! ATGTTGGTTTTTGTGTGTGT+AGG + chr4_4:56989591-56989610 Msa0689770:intron 35.0%
!! TAGGATTACTTTTACTCCGA+CGG + chr4_4:56989610-56989629 Msa0689770:intron 35.0%
TGTAGTATGGTTACTGTGAT+TGG + chr4_4:56989641-56989660 Msa0689770:three_prime_UTR 35.0%
GTGATTTATGTTTAGCTGGT+TGG + chr4_4:56989808-56989827 Msa0689770:intron 35.0%
!!! CGCAAATTTTAGCTTCAAGT+TGG + chr4_4:56989961-56989980 Msa0689770:intron 35.0%
!!! GCTTCAAGTTGGAATCATTT+TGG + chr4_4:56989972-56989991 Msa0689770:intron 35.0%
!!! TCAAGTTGGAATCATTTTGG+AGG + chr4_4:56989975-56989994 Msa0689770:intron 35.0%
GTTCTCAGGTAGAATTGAAT+TGG - chr4_4:56990001-56990020 None:intergenic 35.0%
ACATACACTTAGATGTTCTC+AGG - chr4_4:56990015-56990034 None:intergenic 35.0%
! AGAACTGATTTAGACATGTC+TGG + chr4_4:56990078-56990097 Msa0689770:intron 35.0%
TGCAATGTGTTGTGAATTGA+TGG + chr4_4:56990671-56990690 Msa0689770:intron 35.0%
ATGTGTTGTGAATTGATGGA+TGG + chr4_4:56990675-56990694 Msa0689770:intron 35.0%
! TTTTAGCCAAAGCTACACTT+TGG - chr4_4:56990801-56990820 None:intergenic 35.0%
!!! GTTGTTTTGAAAGTCAAGGA+CGG - chr4_4:56990974-56990993 None:intergenic 35.0%
! AACTCAATCTCCACAAGTTT+TGG - chr4_4:56990996-56991015 None:intergenic 35.0%
! GGAGTATCATGTTTGATTCT+TGG + chr4_4:56991312-56991331 Msa0689770:intron 35.0%
!!! ATGTGTCTGTTTTAACCATC+CGG - chr4_4:56991410-56991429 None:intergenic 35.0%
ATAGTCAAGTATGATAACCG+TGG - chr4_4:56991789-56991808 None:intergenic 35.0%
!! CTATTCTAGCTTCTTAACTG+TGG + chr4_4:56991883-56991902 Msa0689770:intron 35.0%
AAACTTGCACACATGAAGTT+CGG - chr4_4:56991923-56991942 None:intergenic 35.0%
GCAAGTTTGTAGTATCTCTA+TGG + chr4_4:56991935-56991954 Msa0689770:intron 35.0%
AGTTTGTAGTATCTCTATGG+AGG + chr4_4:56991938-56991957 Msa0689770:intron 35.0%
AGTAACGTCACAAACTCATT+TGG - chr4_4:56991988-56992007 None:intergenic 35.0%
AATAGGCACATCAAATACAG+GGG - chr4_4:56992049-56992068 None:intergenic 35.0%
CAATAGGCACATCAAATACA+GGG - chr4_4:56992050-56992069 None:intergenic 35.0%
AAACAAAGAAGAAAGGGGTT+GGG - chr4_4:56992353-56992372 None:intergenic 35.0%
AAAACAAAGAAGAAAGGGGT+TGG - chr4_4:56992354-56992373 None:intergenic 35.0%
GTGTTGAATGCAGATAAAGA+AGG - chr4_4:56992386-56992405 None:intergenic 35.0%
CTTTATCTGCATTCAACACT+AGG + chr4_4:56992387-56992406 Msa0689770:intron 35.0%
TTGTTAGAAGTTCATGTTGC+AGG - chr4_4:56992468-56992487 None:intergenic 35.0%
!! CTTTAGTTCTTTGGTTTCCA+TGG + chr4_4:56992683-56992702 Msa0689770:three_prime_UTR 35.0%
!! CTCATGTTAAGCTTTTTAGG+TGG + chr4_4:56992877-56992896 Msa0689770:three_prime_UTR 35.0%
!!! TCTTTTTTGGCGTGGACTAT+AGG - chr4_4:56989232-56989251 None:intergenic 40.0%
!!! GAATTCGTTCTTTTTTGGCG+TGG - chr4_4:56989240-56989259 None:intergenic 40.0%
!!! GCGAGGAATTCGTTCTTTTT+TGG - chr4_4:56989245-56989264 None:intergenic 40.0%
ATGATCAGAGTGAGGTTCTT+CGG - chr4_4:56989269-56989288 None:intergenic 40.0%
TCAACGAAATGATCAGAGTG+AGG - chr4_4:56989277-56989296 None:intergenic 40.0%
!! CTGTTGATGACAAATTGGGT+CGG + chr4_4:56989500-56989519 Msa0689770:three_prime_UTR 40.0%
CACTTGGATACATCCATGTA+AGG - chr4_4:56990222-56990241 None:intergenic 40.0%
! AGATTTTGCCTCCAGTTAAC+TGG + chr4_4:56990400-56990419 Msa0689770:intron 40.0%
TTCTTAGTTTACTCCGTGCA+TGG + chr4_4:56990723-56990742 Msa0689770:intron 40.0%
TGTGGAGATTGAGTTTGAGT+AGG + chr4_4:56991001-56991020 Msa0689770:intron 40.0%
! TTGAAAGAGTGGAGCTGTTT+TGG + chr4_4:56991070-56991089 Msa0689770:intron 40.0%
! TTACTAAGGTTGCTTTCCCT+CGG + chr4_4:56991382-56991401 Msa0689770:intron 40.0%
! TCTAGCTTCTTAACTGTGGT+AGG + chr4_4:56991887-56991906 Msa0689770:intron 40.0%
AACTTGCACACATGAAGTTC+GGG - chr4_4:56991922-56991941 None:intergenic 40.0%
! TTATTGTGCCGTTGTTCACA+TGG + chr4_4:56992011-56992030 Msa0689770:intron 40.0%
CCAATAGGCACATCAAATAC+AGG - chr4_4:56992051-56992070 None:intergenic 40.0%
CCTGTATTTGATGTGCCTAT+TGG + chr4_4:56992048-56992067 Msa0689770:intron 40.0%
GATTCATCATCAAGAGATGG+GGG - chr4_4:56992099-56992118 None:intergenic 40.0%
GGATTCATCATCAAGAGATG+GGG - chr4_4:56992100-56992119 None:intergenic 40.0%
GGGATTCATCATCAAGAGAT+GGG - chr4_4:56992101-56992120 None:intergenic 40.0%
AGGGATTCATCATCAAGAGA+TGG - chr4_4:56992102-56992121 None:intergenic 40.0%
CTCAAATTCTTCTTTGCAGG+TGG + chr4_4:56992236-56992255 Msa0689770:intron 40.0%
! GTGGACATCAAAGTATCTGA+AGG + chr4_4:56992255-56992274 Msa0689770:three_prime_UTR 40.0%
TCTCATGCCAATGAAGAATC+AGG + chr4_4:56992279-56992298 Msa0689770:three_prime_UTR 40.0%
CTTCACAATAGTTATTGGGC+AGG - chr4_4:56992621-56992640 None:intergenic 40.0%
CTGCCCAATAACTATTGTGA+AGG + chr4_4:56992619-56992638 Msa0689770:three_prime_UTR 40.0%
AAAGGGGAAAAAACAACCCA+TGG - chr4_4:56992703-56992722 None:intergenic 40.0%
AACATGAGCTTGGAGTATGA+GGG - chr4_4:56992865-56992884 None:intergenic 40.0%
TAACATGAGCTTGGAGTATG+AGG - chr4_4:56992866-56992885 None:intergenic 40.0%
GTCATCGCTTTCAAGACTGA+CGG - chr4_4:56989187-56989206 None:intergenic 45.0%
GTCTTGAAAGCGATGACACT+TGG + chr4_4:56989190-56989209 Msa0689770:exon 45.0%
! ATACTACAGTGTCGAACCGT+CGG - chr4_4:56989629-56989648 None:intergenic 45.0%
TTTGCCTCCAGTTAACTGGT+TGG + chr4_4:56990404-56990423 Msa0689770:intron 45.0%
AGAACCAACCAGTTAACTGG+AGG - chr4_4:56990411-56990430 None:intergenic 45.0%
GCTAGAACCAACCAGTTAAC+TGG - chr4_4:56990414-56990433 None:intergenic 45.0%
! TTACTCCGTGCATGGATTGA+CGG + chr4_4:56990731-56990750 Msa0689770:intron 45.0%
GAAGCTCCAAAGTGTAGCTT+TGG + chr4_4:56990792-56990811 Msa0689770:intron 45.0%
! TTTTAACCATCCGGTTCCGA+GGG - chr4_4:56991401-56991420 None:intergenic 45.0%
GACTCGTACATGTGATCACT+AGG + chr4_4:56991590-56991609 Msa0689770:intron 45.0%
CATGTGATCACTAGGTCACA+TGG + chr4_4:56991598-56991617 Msa0689770:intron 45.0%
TTGATGTGCCTATTGGCAAG+TGG + chr4_4:56992055-56992074 Msa0689770:intron 45.0%
GACACATACCACTTGCCAAT+AGG - chr4_4:56992066-56992085 None:intergenic 45.0%
! AGAGAGTGTGCAAGTGAGAA+GGG - chr4_4:56992121-56992140 None:intergenic 45.0%
CTCGTGAATCACGATTCTCA+AGG + chr4_4:56992141-56992160 Msa0689770:intron 45.0%
GGTTGGGAAAAGCTTAGTCT+TGG - chr4_4:56992337-56992356 None:intergenic 45.0%
!! AACATGATGTGGGGTTGGTT+GGG - chr4_4:56992729-56992748 None:intergenic 45.0%
! AAACATGATGTGGGGTTGGT+TGG - chr4_4:56992730-56992749 None:intergenic 45.0%
TGCTAAACATGATGTGGGGT+TGG - chr4_4:56992734-56992753 None:intergenic 45.0%
ACCCCACATCATGTTTAGCA+TGG + chr4_4:56992734-56992753 Msa0689770:three_prime_UTR 45.0%
CCCATGCTAAACATGATGTG+GGG - chr4_4:56992738-56992757 None:intergenic 45.0%
CCCCACATCATGTTTAGCAT+GGG + chr4_4:56992735-56992754 Msa0689770:three_prime_UTR 45.0%
CCCCATGCTAAACATGATGT+GGG - chr4_4:56992739-56992758 None:intergenic 45.0%
CCCACATCATGTTTAGCATG+GGG + chr4_4:56992736-56992755 Msa0689770:three_prime_UTR 45.0%
CCACATCATGTTTAGCATGG+GGG + chr4_4:56992737-56992756 Msa0689770:three_prime_UTR 45.0%
GACGGTTCGACACTGTAGTA+TGG + chr4_4:56989628-56989647 Msa0689770:three_prime_UTR 50.0%
GAAGCGTGTCTTTGCTTCTG+CGG - chr4_4:56990325-56990344 None:intergenic 50.0%
ACTCACCGTCAATCCATGCA+CGG - chr4_4:56990739-56990758 None:intergenic 50.0%
! GCATGGATTGACGGTGAGTT+TGG + chr4_4:56990740-56990759 Msa0689770:intron 50.0%
GTGCAATTCCACCAAACTCG+CGG + chr4_4:56990836-56990855 Msa0689770:intron 50.0%
GTTTGGATTGACCGCGAGTT+TGG - chr4_4:56990850-56990869 None:intergenic 50.0%
!! GTTTTAACCATCCGGTTCCG+AGG - chr4_4:56991402-56991421 None:intergenic 50.0%
! GAGAGAGTGTGCAAGTGAGA+AGG - chr4_4:56992122-56992141 None:intergenic 50.0%
GCGCAGGTTTGGATTCTCTA+AGG - chr4_4:56992543-56992562 None:intergenic 50.0%
!! TGGGGTTGGTTGGGAAGAAA+GGG - chr4_4:56992720-56992739 None:intergenic 50.0%
CCCCCATGCTAAACATGATG+TGG - chr4_4:56992740-56992759 None:intergenic 50.0%
TGGATTGACCGCGAGTTTGG+TGG - chr4_4:56990847-56990866 None:intergenic 55.0%
! AGGTTGCTTTCCCTCGGAAC+CGG + chr4_4:56991388-56991407 Msa0689770:intron 55.0%
CGAGACACTCATCCACAAGC+TGG - chr4_4:56992565-56992584 None:intergenic 55.0%
!! GGGGTTGGTTGGGAAGAAAG+GGG - chr4_4:56992719-56992738 None:intergenic 55.0%
!! GTGGGGTTGGTTGGGAAGAA+AGG - chr4_4:56992721-56992740 None:intergenic 55.0%
TGAGCTTGGAGTATGAGGGC+TGG - chr4_4:56992861-56992880 None:intergenic 55.0%
GAGTGAGGTTCTTCGGAGCG+AGG - chr4_4:56989262-56989281 None:intergenic 60.0%
TGCTTTCCCTCGGAACCGGA+TGG + chr4_4:56991392-56991411 Msa0689770:intron 60.0%
TCCAAACCTGCGCCAGCTTG+TGG + chr4_4:56992550-56992569 Msa0689770:three_prime_UTR 60.0%
TCCACAAGCTGGCGCAGGTT+TGG - chr4_4:56992554-56992573 None:intergenic 60.0%
ACTCATCCACAAGCTGGCGC+AGG - chr4_4:56992559-56992578 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 56989054 56992929 56989054 ID=Msa0689770;Name=Msa0689770
chr4_4 mRNA 56989054 56992929 56989054 ID=Msa0689770-mRNA-1;Parent=Msa0689770;Name=Msa0689770-mRNA-1;_AED=0.03;_eAED=0.03;_QI=148|0.66|0.75|1|1|1|4|685|59
chr4_4 exon 56989054 56989527 56989054 ID=Msa0689770-mRNA-1:exon:15176;Parent=Msa0689770-mRNA-1
chr4_4 exon 56989613 56989676 56989613 ID=Msa0689770-mRNA-1:exon:15177;Parent=Msa0689770-mRNA-1
chr4_4 exon 56992255 56992300 56992255 ID=Msa0689770-mRNA-1:exon:15178;Parent=Msa0689770-mRNA-1
chr4_4 exon 56992501 56992929 56992501 ID=Msa0689770-mRNA-1:exon:15179;Parent=Msa0689770-mRNA-1
chr4_4 five_prime_UTR 56989054 56989201 56989054 ID=Msa0689770-mRNA-1:five_prime_utr;Parent=Msa0689770-mRNA-1
chr4_4 CDS 56989202 56989381 56989202 ID=Msa0689770-mRNA-1:cds;Parent=Msa0689770-mRNA-1
chr4_4 three_prime_UTR 56989382 56989527 56989382 ID=Msa0689770-mRNA-1:three_prime_utr;Parent=Msa0689770-mRNA-1
chr4_4 three_prime_UTR 56989613 56989676 56989613 ID=Msa0689770-mRNA-1:three_prime_utr;Parent=Msa0689770-mRNA-1
chr4_4 three_prime_UTR 56992255 56992300 56992255 ID=Msa0689770-mRNA-1:three_prime_utr;Parent=Msa0689770-mRNA-1
chr4_4 three_prime_UTR 56992501 56992929 56992501 ID=Msa0689770-mRNA-1:three_prime_utr;Parent=Msa0689770-mRNA-1
Gene Sequence

>Msa0689770

ATGACACTTGGTTTGATCAATGCTAATCCTATAGTCCACGCCAAAAAAGAACGAATTCCTCGCTCCGAAGAACCTCACTCTGATCATTTCGTTGATCCTCTCGATATTTATGATATCCTTCTTTCATTTTTTTTTTATTTCTCTTCATTTATATATTTTCTCTATGTTTACTTGAATTGA
Protein sequence

>Msa0689770

MTLGLINANPIVHAKKERIPRSEEPHSDHFVDPLDIYDILLSFFFYFSSFIYFLYVYLN*