AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1420260

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1420260 MtrunA17_Chr5g0404041 87.500 48 6 0 1 48 1 48 3.66e-25 87.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1420260 AT3G50845.1 65.789 38 13 0 1 38 1 38 2.02e-11 55.5

Find 52 sgRNAs with CRISPR-Local

Find 204 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTTCTGTTGATGACAAAT+TGG 0.159295 tig0024770:+53865 Msa1420260:three_prime_UTR
CAAATTTCGTGAGAGATATT+AGG 0.180944 tig0024770:+53789 Msa1420260:three_prime_UTR
AATGCCTTCACAATAGTTAT+TGG 0.230337 tig0024770:-56992 None:intergenic
TCATTATGTCTTTAGTTCTT+TGG 0.260015 tig0024770:+57043 Msa1420260:three_prime_UTR
AGTTTGCATCATTTCGCTTT+TGG 0.271870 tig0024770:-53458 None:intergenic
CTTTAGTTCTTTGGTTTCCA+TGG 0.295092 tig0024770:+57052 Msa1420260:three_prime_UTR
TTAAGCTGTTCCAATGAATA+AGG 0.330883 tig0024770:-53824 None:intergenic
TATGTTTGTTCACAGTTCAT+TGG 0.347534 tig0024770:-56957 None:intergenic
AACTTTACCTGATTCTTCAT+TGG 0.354188 tig0024770:-56656 None:intergenic
AAATTTCGTGAGAGATATTA+GGG 0.354298 tig0024770:+53790 Msa1420260:three_prime_UTR
TCCAATGAATAAGGATGTTC+AGG 0.357418 tig0024770:-53815 None:intergenic
TCCTGAACATCCTTATTCAT+TGG 0.366089 tig0024770:+53814 Msa1420260:three_prime_UTR
ATGCCTTCACAATAGTTATT+GGG 0.370934 tig0024770:-56991 None:intergenic
TCCACAAGCTGGCGCAGGTT+TGG 0.379145 tig0024770:-56920 None:intergenic
CAATTTGTCATCAACAGAAA+TGG 0.383548 tig0024770:-53864 None:intergenic
GTGGGGTTGGTTGGGAAGAA+AGG 0.391030 tig0024770:-57087 None:intergenic
ATTTCTGTTGATGACAAATT+GGG 0.401971 tig0024770:+53866 Msa1420260:three_prime_UTR
GCGCAGGTTTGGATTCTCTA+AGG 0.406934 tig0024770:-56909 None:intergenic
TAACATGAGCTTGGAGTATG+AGG 0.410865 tig0024770:-57232 None:intergenic
TGGGGTTGGTTGGGAAGAAA+GGG 0.413412 tig0024770:-57086 None:intergenic
TTTAGTTCTTTGGTTTCCAT+GGG 0.424840 tig0024770:+57053 Msa1420260:three_prime_UTR
TCTCATGCCAATGAAGAATC+AGG 0.430582 tig0024770:+56649 Msa1420260:three_prime_UTR
ATGATCAGAGTGAGGTTCTT+CGG 0.436626 tig0024770:-53636 None:intergenic
GACGGTTCGACACTGTAGTA+TGG 0.437697 tig0024770:+53998 Msa1420260:three_prime_UTR
GTCTTGAAAGCGATGACACT+TGG 0.447355 tig0024770:+53560 Msa1420260:exon
AACATGATGTGGGGTTGGTT+GGG 0.451572 tig0024770:-57095 None:intergenic
TCCAAACCTGCGCCAGCTTG+TGG 0.483104 tig0024770:+56919 Msa1420260:three_prime_UTR
TGTAGTATGGTTACTGTGAT+TGG 0.485275 tig0024770:+54011 Msa1420260:three_prime_UTR
AAACATGATGTGGGGTTGGT+TGG 0.497453 tig0024770:-57096 None:intergenic
GGGGTTGGTTGGGAAGAAAG+GGG 0.509817 tig0024770:-57085 None:intergenic
CTTCACAATAGTTATTGGGC+AGG 0.517991 tig0024770:-56987 None:intergenic
TATTTGATGATTGATGATGA+TGG 0.518913 tig0024770:-53501 None:intergenic
GTCAAATCAAATCAAAGTCA+AGG 0.520569 tig0024770:-53767 None:intergenic
CCCCACATCATGTTTAGCAT+GGG 0.522342 tig0024770:+57104 Msa1420260:three_prime_UTR
ACCCCACATCATGTTTAGCA+TGG 0.552675 tig0024770:+57103 Msa1420260:three_prime_UTR
CCCCATGCTAAACATGATGT+GGG 0.557277 tig0024770:-57105 None:intergenic
CTGTTGATGACAAATTGGGT+CGG 0.557673 tig0024770:+53870 Msa1420260:three_prime_UTR
TGCTAAACATGATGTGGGGT+TGG 0.576444 tig0024770:-57100 None:intergenic
TGAGCTTGGAGTATGAGGGC+TGG 0.583758 tig0024770:-57227 None:intergenic
ATACTACAGTGTCGAACCGT+CGG 0.586741 tig0024770:-53996 None:intergenic
CGAGACACTCATCCACAAGC+TGG 0.593597 tig0024770:-56931 None:intergenic
CTGCCCAATAACTATTGTGA+AGG 0.600420 tig0024770:+56988 Msa1420260:three_prime_UTR
ACTCATCCACAAGCTGGCGC+AGG 0.607822 tig0024770:-56925 None:intergenic
GAGTGAGGTTCTTCGGAGCG+AGG 0.620124 tig0024770:-53629 None:intergenic
GTGGACATCAAAGTATCTGA+AGG 0.621459 tig0024770:+56625 Msa1420260:three_prime_UTR
CCCACATCATGTTTAGCATG+GGG 0.632974 tig0024770:+57105 Msa1420260:three_prime_UTR
GTCATCGCTTTCAAGACTGA+CGG 0.633845 tig0024770:-53554 None:intergenic
AACATGAGCTTGGAGTATGA+GGG 0.637702 tig0024770:-57231 None:intergenic
AGGATATCATAAATATCGAG+AGG 0.652332 tig0024770:-53668 None:intergenic
TCAACGAAATGATCAGAGTG+AGG 0.689074 tig0024770:-53644 None:intergenic
CCCATGCTAAACATGATGTG+GGG 0.709124 tig0024770:-57104 None:intergenic
CCACATCATGTTTAGCATGG+GGG 0.714707 tig0024770:+57106 Msa1420260:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAAAAAAAATGAAAGA+AGG - tig0024770:53691-53710 None:intergenic 10.0%
!! AATTTGAATTGAATTTATGT+TGG + tig0024770:53945-53964 Msa1420260:intron 15.0%
!! AAAAAAAAACAAAGAAGAAA+GGG - tig0024770:56729-56748 None:intergenic 15.0%
!!! TAAAATGTTAAAGTTTGTTT+GGG - tig0024770:56800-56819 None:intergenic 15.0%
!!! ATAAAATGTTAAAGTTTGTT+TGG - tig0024770:56801-56820 None:intergenic 15.0%
!!! TTCTAGTTATAAGTATGTTT+TGG + tig0024770:54201-54220 Msa1420260:intron 20.0%
!!! TTCATCTTCAACTTATTTTT+AGG + tig0024770:54620-54639 Msa1420260:intron 20.0%
!!! AACTTAAGCATTTTTAAAGT+AGG + tig0024770:55486-55505 Msa1420260:intron 20.0%
!! ATTCAAATTTACACAACATT+TGG - tig0024770:55553-55572 None:intergenic 20.0%
!!! AACCTTAGTAATCTAAAATT+TGG - tig0024770:55743-55762 None:intergenic 20.0%
!!! ATTAGTTTATTATTCAGAGA+AGG + tig0024770:55884-55903 Msa1420260:intron 20.0%
!! TTAGTTTATTATTCAGAGAA+GGG + tig0024770:55885-55904 Msa1420260:intron 20.0%
!! TTGACTATAATTATGACTTT+GGG + tig0024770:56171-56190 Msa1420260:intron 20.0%
!! AAAAAAAACAAAGAAGAAAG+GGG - tig0024770:56728-56747 None:intergenic 20.0%
!! GAAAAAAAAACAAAGAAGAA+AGG - tig0024770:56730-56749 None:intergenic 20.0%
!!! TATTTGATGATTGATGATGA+TGG - tig0024770:53504-53523 None:intergenic 25.0%
! AAATTTCGTGAGAGATATTA+GGG + tig0024770:53790-53809 Msa1420260:three_prime_UTR 25.0%
!!! ATTTCTGTTGATGACAAATT+GGG + tig0024770:53866-53885 Msa1420260:three_prime_UTR 25.0%
!! TAATAACTTGTGAAGCAAAA+GGG - tig0024770:54100-54119 None:intergenic 25.0%
!! ATAATAACTTGTGAAGCAAA+AGG - tig0024770:54101-54120 None:intergenic 25.0%
! TATAGTGATTTATGTTTAGC+TGG + tig0024770:54174-54193 Msa1420260:intron 25.0%
!! GGCTGAAAATTAATTTTAGA+AGG + tig0024770:54301-54320 Msa1420260:intron 25.0%
!!! TGTATGTTTTGTTTCACTTT+TGG + tig0024770:54398-54417 Msa1420260:intron 25.0%
! TAAGTTAGAATCAATTCTGA+AGG - tig0024770:54500-54519 None:intergenic 25.0%
!!! ACAAAAATTGATTTTGTGTG+TGG - tig0024770:54670-54689 None:intergenic 25.0%
! TGAGTCAAAATTGATTATAG+AGG - tig0024770:55001-55020 None:intergenic 25.0%
!!! AATTTTGACTCATTCAAAAG+TGG + tig0024770:55009-55028 Msa1420260:intron 25.0%
! TAAATGTCCTATATGGAAAT+TGG + tig0024770:55313-55332 Msa1420260:intron 25.0%
!!! GAGAAATTTATTTTACGAAG+TGG + tig0024770:55613-55632 Msa1420260:intron 25.0%
!!! ATTTATTTTACGAAGTGGTT+AGG + tig0024770:55618-55637 Msa1420260:intron 25.0%
!! TGATTAATGAGCTTTAGTTA+AGG + tig0024770:55647-55666 Msa1420260:intron 25.0%
!! TAATCTAAAATTTGGCTAAG+AGG - tig0024770:55735-55754 None:intergenic 25.0%
!! AGCCAAATTTTAGATTACTA+AGG + tig0024770:55738-55757 Msa1420260:intron 25.0%
! ATTAAGAAGTGGAAACAAAA+GGG + tig0024770:55843-55862 Msa1420260:intron 25.0%
! TGTTACCTTGTATTTGTAAT+AGG + tig0024770:55926-55945 Msa1420260:intron 25.0%
! TTCAACCTATTACAAATACA+AGG - tig0024770:55934-55953 None:intergenic 25.0%
!! GTTTTCAAATTATGTTTCCA+CGG + tig0024770:56139-56158 Msa1420260:intron 25.0%
! CTTGACTATAATTATGACTT+TGG + tig0024770:56170-56189 Msa1420260:intron 25.0%
! ACTATATGATATAAGTAGTG+TGG - tig0024770:56551-56570 None:intergenic 25.0%
! TATAGTATAATTGTCTTCTC+TGG + tig0024770:56565-56584 Msa1420260:intron 25.0%
!!! TCATTATGTCTTTAGTTCTT+TGG + tig0024770:57043-57062 Msa1420260:three_prime_UTR 25.0%
!!! TAGGTGGTAAGTTTTAAAAA+TGG + tig0024770:57262-57281 Msa1420260:three_prime_UTR 25.0%
AGGATATCATAAATATCGAG+AGG - tig0024770:53671-53690 None:intergenic 30.0%
GTCAAATCAAATCAAAGTCA+AGG - tig0024770:53770-53789 None:intergenic 30.0%
CAAATTTCGTGAGAGATATT+AGG + tig0024770:53789-53808 Msa1420260:three_prime_UTR 30.0%
TTAAGCTGTTCCAATGAATA+AGG - tig0024770:53827-53846 None:intergenic 30.0%
CAATTTGTCATCAACAGAAA+TGG - tig0024770:53867-53886 None:intergenic 30.0%
!! CATTTCTGTTGATGACAAAT+TGG + tig0024770:53865-53884 Msa1420260:three_prime_UTR 30.0%
AAACAAACTTTGTAATGAGC+AGG - tig0024770:54071-54090 None:intergenic 30.0%
! GAAAAACTGATTCTAGCTAT+AGG + tig0024770:54257-54276 Msa1420260:intron 30.0%
! TGAGTTGAACACTAGATTAA+AGG + tig0024770:54280-54299 Msa1420260:intron 30.0%
CAACTTGAAGCTAAAATTTG+CGG - tig0024770:54333-54352 None:intergenic 30.0%
GTAGATAAATTTGACTCTAG+AGG + tig0024770:54422-54441 Msa1420260:intron 30.0%
ATTGTTCAACTAACCTTACA+TGG + tig0024770:54576-54595 Msa1420260:intron 30.0%
AAGATGAAGTAGTTTACACT+TGG - tig0024770:54608-54627 None:intergenic 30.0%
!! ATTTTGTGTGTGGATTGATT+TGG - tig0024770:54660-54679 None:intergenic 30.0%
! ATAAGTGTATTTCGTTTTGC+AGG + tig0024770:54805-54824 Msa1420260:intron 30.0%
! TAAGTGTATTTCGTTTTGCA+GGG + tig0024770:54806-54825 Msa1420260:intron 30.0%
TCTAACTTGAAGCTACAATT+TGG - tig0024770:54948-54967 None:intergenic 30.0%
CAAGTTAGAATCAATTCTGA+AGG + tig0024770:54959-54978 Msa1420260:intron 30.0%
! AGCTTTGGCTAAAAAATCAT+TGG + tig0024770:55177-55196 Msa1420260:intron 30.0%
GCTTTGGCTAAAAAATCATT+GGG + tig0024770:55178-55197 Msa1420260:intron 30.0%
CTTTGGCTAAAAAATCATTG+GGG + tig0024770:55179-55198 Msa1420260:intron 30.0%
! ACTTAATGGATGTGTATGTT+TGG - tig0024770:55237-55256 None:intergenic 30.0%
AAACATACACATCCATTAAG+TGG + tig0024770:55236-55255 Msa1420260:intron 30.0%
AAAGCATACAAACCACTTAA+TGG - tig0024770:55251-55270 None:intergenic 30.0%
ATAGGACATTTATGAGGTTA+TGG - tig0024770:55305-55324 None:intergenic 30.0%
AACCTCATAAATGTCCTATA+TGG + tig0024770:55306-55325 Msa1420260:intron 30.0%
TTCCATATAGGACATTTATG+AGG - tig0024770:55311-55330 None:intergenic 30.0%
GGATAAACCAATTTCCATAT+AGG - tig0024770:55323-55342 None:intergenic 30.0%
!!! TTTGGTTGTTTTGAAAGTCA+AGG - tig0024770:55348-55367 None:intergenic 30.0%
TTCAAAACAACCAAAACTTG+TGG + tig0024770:55353-55372 Msa1420260:intron 30.0%
!! TTCTCTTTCTTTTGAAAGAG+TGG + tig0024770:55429-55448 Msa1420260:intron 30.0%
! AGCTTTAGTTAAGGTTGAAT+TGG + tig0024770:55656-55675 Msa1420260:intron 30.0%
TTAGTTAAGGTTGAATTGGT+TGG + tig0024770:55660-55679 Msa1420260:intron 30.0%
TAGTTAAGGTTGAATTGGTT+GGG + tig0024770:55661-55680 Msa1420260:intron 30.0%
!! ATAGTAGAAGCATTAAGAAG+TGG + tig0024770:55832-55851 Msa1420260:intron 30.0%
CATTAAGAAGTGGAAACAAA+AGG + tig0024770:55842-55861 Msa1420260:intron 30.0%
TTAAGAAGTGGAAACAAAAG+GGG + tig0024770:55844-55863 Msa1420260:intron 30.0%
TAACTTCATTACAGTGTACA+CGG - tig0024770:56213-56232 None:intergenic 30.0%
ATATCAAACCATGTGAACAA+CGG - tig0024770:56392-56411 None:intergenic 30.0%
TTACTCAAATTCTTCTTTGC+AGG + tig0024770:56603-56622 Msa1420260:intron 30.0%
AACTTTACCTGATTCTTCAT+TGG - tig0024770:56659-56678 None:intergenic 30.0%
TGTTGAATGCAGATAAAGAA+GGG - tig0024770:56754-56773 None:intergenic 30.0%
! AGTTTGTTTGGGAATTTAGA+TGG - tig0024770:56789-56808 None:intergenic 30.0%
TATGTTTGTTCACAGTTCAT+TGG - tig0024770:56960-56979 None:intergenic 30.0%
ATGCCTTCACAATAGTTATT+GGG - tig0024770:56994-57013 None:intergenic 30.0%
AATGCCTTCACAATAGTTAT+TGG - tig0024770:56995-57014 None:intergenic 30.0%
!! TTTAGTTCTTTGGTTTCCAT+GGG + tig0024770:57053-57072 Msa1420260:three_prime_UTR 30.0%
!!! TCTGTATATTTTGCTGTTTG+TGG + tig0024770:57198-57217 Msa1420260:three_prime_UTR 30.0%
!!! CTGTATATTTTGCTGTTTGT+GGG + tig0024770:57199-57218 Msa1420260:three_prime_UTR 30.0%
TAAAAAGCTTAACATGAGCT+TGG - tig0024770:57244-57263 None:intergenic 30.0%
! AAGCTCATGTTAAGCTTTTT+AGG + tig0024770:57243-57262 Msa1420260:three_prime_UTR 30.0%
! AGTTTGCATCATTTCGCTTT+TGG - tig0024770:53461-53480 None:intergenic 35.0%
TCCTGAACATCCTTATTCAT+TGG + tig0024770:53814-53833 Msa1420260:three_prime_UTR 35.0%
TCCAATGAATAAGGATGTTC+AGG - tig0024770:53818-53837 None:intergenic 35.0%
!!! ATGTTGGTTTTTGTGTGTGT+AGG + tig0024770:53961-53980 Msa1420260:intron 35.0%
!! TAGGATTACTTTTACTCCGA+CGG + tig0024770:53980-53999 Msa1420260:intron 35.0%
TGTAGTATGGTTACTGTGAT+TGG + tig0024770:54011-54030 Msa1420260:three_prime_UTR 35.0%
GTGATTTATGTTTAGCTGGT+TGG + tig0024770:54178-54197 Msa1420260:intron 35.0%
!!! CGCAAATTTTAGCTTCAAGT+TGG + tig0024770:54331-54350 Msa1420260:intron 35.0%
!!! GCTTCAAGTTGGAATCATTT+TGG + tig0024770:54342-54361 Msa1420260:intron 35.0%
!!! TCAAGTTGGAATCATTTTGG+AGG + tig0024770:54345-54364 Msa1420260:intron 35.0%
GTTCTCAGGTAGAATTGAAT+TGG - tig0024770:54371-54390 None:intergenic 35.0%
ACATACACTTAGATGTTCTC+AGG - tig0024770:54385-54404 None:intergenic 35.0%
! AGAACTGATTTAGACATGTC+TGG + tig0024770:54448-54467 Msa1420260:intron 35.0%
TGCAATGTGTTGTGAATTGA+TGG + tig0024770:55041-55060 Msa1420260:intron 35.0%
ATGTGTTGTGAATTGATGGA+TGG + tig0024770:55045-55064 Msa1420260:intron 35.0%
! TTTTAGCCAAAGCTACACTT+TGG - tig0024770:55171-55190 None:intergenic 35.0%
!!! GTTGTTTTGAAAGTCAAGGA+CGG - tig0024770:55344-55363 None:intergenic 35.0%
! AACTCAATCTCCACAAGTTT+TGG - tig0024770:55366-55385 None:intergenic 35.0%
! GGAGTATCATGTTTGATTCT+TGG + tig0024770:55682-55701 Msa1420260:intron 35.0%
!!! ATGTGTCTGTTTTAACCATC+CGG - tig0024770:55780-55799 None:intergenic 35.0%
ATAGTCAAGTATGATAACCG+TGG - tig0024770:56159-56178 None:intergenic 35.0%
!! CTATTCTAGCTTCTTAACTG+TGG + tig0024770:56253-56272 Msa1420260:intron 35.0%
AAACTTGCACACATGAAGTT+CGG - tig0024770:56293-56312 None:intergenic 35.0%
GCAAGTTTGTAGTATCTCTA+TGG + tig0024770:56305-56324 Msa1420260:intron 35.0%
AGTTTGTAGTATCTCTATGG+AGG + tig0024770:56308-56327 Msa1420260:intron 35.0%
AGTAACGTCACAAACTCATT+TGG - tig0024770:56358-56377 None:intergenic 35.0%
AATAGGCACATCAAATACAG+GGG - tig0024770:56419-56438 None:intergenic 35.0%
CAATAGGCACATCAAATACA+GGG - tig0024770:56420-56439 None:intergenic 35.0%
AAACAAAGAAGAAAGGGGTT+GGG - tig0024770:56723-56742 None:intergenic 35.0%
AAAACAAAGAAGAAAGGGGT+TGG - tig0024770:56724-56743 None:intergenic 35.0%
GTGTTGAATGCAGATAAAGA+AGG - tig0024770:56755-56774 None:intergenic 35.0%
CTTTATCTGCATTCAACACT+AGG + tig0024770:56756-56775 Msa1420260:intron 35.0%
TTGTTAGAAGTTCATGTTGC+AGG - tig0024770:56837-56856 None:intergenic 35.0%
!! CTTTAGTTCTTTGGTTTCCA+TGG + tig0024770:57052-57071 Msa1420260:three_prime_UTR 35.0%
!! CTCATGTTAAGCTTTTTAGG+TGG + tig0024770:57246-57265 Msa1420260:three_prime_UTR 35.0%
!!! TCTTTTTTGGCGTGGACTAT+AGG - tig0024770:53602-53621 None:intergenic 40.0%
!!! GAATTCGTTCTTTTTTGGCG+TGG - tig0024770:53610-53629 None:intergenic 40.0%
!!! GCGAGGAATTCGTTCTTTTT+TGG - tig0024770:53615-53634 None:intergenic 40.0%
ATGATCAGAGTGAGGTTCTT+CGG - tig0024770:53639-53658 None:intergenic 40.0%
TCAACGAAATGATCAGAGTG+AGG - tig0024770:53647-53666 None:intergenic 40.0%
!! CTGTTGATGACAAATTGGGT+CGG + tig0024770:53870-53889 Msa1420260:three_prime_UTR 40.0%
CACTTGGATACATCCATGTA+AGG - tig0024770:54592-54611 None:intergenic 40.0%
! AGATTTTGCCTCCAGTTAAC+TGG + tig0024770:54770-54789 Msa1420260:intron 40.0%
TTCTTAGTTTACTCCGTGCA+TGG + tig0024770:55093-55112 Msa1420260:intron 40.0%
TGTGGAGATTGAGTTTGAGT+AGG + tig0024770:55371-55390 Msa1420260:intron 40.0%
! TTGAAAGAGTGGAGCTGTTT+TGG + tig0024770:55440-55459 Msa1420260:intron 40.0%
! TTACTAAGGTTGCTTTCCCT+CGG + tig0024770:55752-55771 Msa1420260:intron 40.0%
! TCTAGCTTCTTAACTGTGGT+AGG + tig0024770:56257-56276 Msa1420260:intron 40.0%
AACTTGCACACATGAAGTTC+GGG - tig0024770:56292-56311 None:intergenic 40.0%
! TTATTGTGCCGTTGTTCACA+TGG + tig0024770:56381-56400 Msa1420260:intron 40.0%
CCAATAGGCACATCAAATAC+AGG - tig0024770:56421-56440 None:intergenic 40.0%
CCTGTATTTGATGTGCCTAT+TGG + tig0024770:56418-56437 Msa1420260:intron 40.0%
GATTCATCATCAAGAGATGG+GGG - tig0024770:56469-56488 None:intergenic 40.0%
GGATTCATCATCAAGAGATG+GGG - tig0024770:56470-56489 None:intergenic 40.0%
GGGATTCATCATCAAGAGAT+GGG - tig0024770:56471-56490 None:intergenic 40.0%
AGGGATTCATCATCAAGAGA+TGG - tig0024770:56472-56491 None:intergenic 40.0%
CTCAAATTCTTCTTTGCAGG+TGG + tig0024770:56606-56625 Msa1420260:intron 40.0%
! GTGGACATCAAAGTATCTGA+AGG + tig0024770:56625-56644 Msa1420260:three_prime_UTR 40.0%
TCTCATGCCAATGAAGAATC+AGG + tig0024770:56649-56668 Msa1420260:three_prime_UTR 40.0%
CTTCACAATAGTTATTGGGC+AGG - tig0024770:56990-57009 None:intergenic 40.0%
CTGCCCAATAACTATTGTGA+AGG + tig0024770:56988-57007 Msa1420260:three_prime_UTR 40.0%
AAAGGGGAAAAAACAACCCA+TGG - tig0024770:57072-57091 None:intergenic 40.0%
AACATGAGCTTGGAGTATGA+GGG - tig0024770:57234-57253 None:intergenic 40.0%
TAACATGAGCTTGGAGTATG+AGG - tig0024770:57235-57254 None:intergenic 40.0%
GTCATCGCTTTCAAGACTGA+CGG - tig0024770:53557-53576 None:intergenic 45.0%
GTCTTGAAAGCGATGACACT+TGG + tig0024770:53560-53579 Msa1420260:exon 45.0%
! ATACTACAGTGTCGAACCGT+CGG - tig0024770:53999-54018 None:intergenic 45.0%
TTTGCCTCCAGTTAACTGGT+TGG + tig0024770:54774-54793 Msa1420260:intron 45.0%
AGAACCAACCAGTTAACTGG+AGG - tig0024770:54781-54800 None:intergenic 45.0%
GCTAGAACCAACCAGTTAAC+TGG - tig0024770:54784-54803 None:intergenic 45.0%
! TTACTCCGTGCATGGATTGA+CGG + tig0024770:55101-55120 Msa1420260:intron 45.0%
GAAGCTCCAAAGTGTAGCTT+TGG + tig0024770:55162-55181 Msa1420260:intron 45.0%
! TTTTAACCATCCGGTTCCGA+GGG - tig0024770:55771-55790 None:intergenic 45.0%
GACTCGTACATGTGATCACT+AGG + tig0024770:55960-55979 Msa1420260:intron 45.0%
CATGTGATCACTAGGTCACA+TGG + tig0024770:55968-55987 Msa1420260:intron 45.0%
TTGATGTGCCTATTGGCAAG+TGG + tig0024770:56425-56444 Msa1420260:intron 45.0%
GACACATACCACTTGCCAAT+AGG - tig0024770:56436-56455 None:intergenic 45.0%
! AGAGAGTGTGCAAGTGAGAA+GGG - tig0024770:56491-56510 None:intergenic 45.0%
CTCGTGAATCACGATTCTCA+AGG + tig0024770:56511-56530 Msa1420260:intron 45.0%
GGTTGGGAAAAGCTTAGTCT+TGG - tig0024770:56707-56726 None:intergenic 45.0%
!! AACATGATGTGGGGTTGGTT+GGG - tig0024770:57098-57117 None:intergenic 45.0%
! AAACATGATGTGGGGTTGGT+TGG - tig0024770:57099-57118 None:intergenic 45.0%
TGCTAAACATGATGTGGGGT+TGG - tig0024770:57103-57122 None:intergenic 45.0%
ACCCCACATCATGTTTAGCA+TGG + tig0024770:57103-57122 Msa1420260:three_prime_UTR 45.0%
CCCATGCTAAACATGATGTG+GGG - tig0024770:57107-57126 None:intergenic 45.0%
CCCCACATCATGTTTAGCAT+GGG + tig0024770:57104-57123 Msa1420260:three_prime_UTR 45.0%
CCCCATGCTAAACATGATGT+GGG - tig0024770:57108-57127 None:intergenic 45.0%
CCCACATCATGTTTAGCATG+GGG + tig0024770:57105-57124 Msa1420260:three_prime_UTR 45.0%
CCACATCATGTTTAGCATGG+GGG + tig0024770:57106-57125 Msa1420260:three_prime_UTR 45.0%
GACGGTTCGACACTGTAGTA+TGG + tig0024770:53998-54017 Msa1420260:three_prime_UTR 50.0%
GAAGCGTGTCTTTGCTTCTG+CGG - tig0024770:54695-54714 None:intergenic 50.0%
ACTCACCGTCAATCCATGCA+CGG - tig0024770:55109-55128 None:intergenic 50.0%
! GCATGGATTGACGGTGAGTT+TGG + tig0024770:55110-55129 Msa1420260:intron 50.0%
GTGCAATTCCACCAAACTCG+CGG + tig0024770:55206-55225 Msa1420260:intron 50.0%
GTTTGGATTGACCGCGAGTT+TGG - tig0024770:55220-55239 None:intergenic 50.0%
!! GTTTTAACCATCCGGTTCCG+AGG - tig0024770:55772-55791 None:intergenic 50.0%
! GAGAGAGTGTGCAAGTGAGA+AGG - tig0024770:56492-56511 None:intergenic 50.0%
GCGCAGGTTTGGATTCTCTA+AGG - tig0024770:56912-56931 None:intergenic 50.0%
!! TGGGGTTGGTTGGGAAGAAA+GGG - tig0024770:57089-57108 None:intergenic 50.0%
CCCCCATGCTAAACATGATG+TGG - tig0024770:57109-57128 None:intergenic 50.0%
TGGATTGACCGCGAGTTTGG+TGG - tig0024770:55217-55236 None:intergenic 55.0%
! AGGTTGCTTTCCCTCGGAAC+CGG + tig0024770:55758-55777 Msa1420260:intron 55.0%
CGAGACACTCATCCACAAGC+TGG - tig0024770:56934-56953 None:intergenic 55.0%
!! GGGGTTGGTTGGGAAGAAAG+GGG - tig0024770:57088-57107 None:intergenic 55.0%
!! GTGGGGTTGGTTGGGAAGAA+AGG - tig0024770:57090-57109 None:intergenic 55.0%
TGAGCTTGGAGTATGAGGGC+TGG - tig0024770:57230-57249 None:intergenic 55.0%
GAGTGAGGTTCTTCGGAGCG+AGG - tig0024770:53632-53651 None:intergenic 60.0%
TGCTTTCCCTCGGAACCGGA+TGG + tig0024770:55762-55781 Msa1420260:intron 60.0%
TCCAAACCTGCGCCAGCTTG+TGG + tig0024770:56919-56938 Msa1420260:three_prime_UTR 60.0%
TCCACAAGCTGGCGCAGGTT+TGG - tig0024770:56923-56942 None:intergenic 60.0%
ACTCATCCACAAGCTGGCGC+AGG - tig0024770:56928-56947 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
tig0024770 gene 53424 57298 53424 ID=Msa1420260;Name=Msa1420260
tig0024770 mRNA 53424 57298 53424 ID=Msa1420260-mRNA-1;Parent=Msa1420260;Name=Msa1420260-mRNA-1;_AED=0.03;_eAED=0.03;_QI=148|0.66|0.75|1|1|1|4|685|59
tig0024770 exon 53424 53897 53424 ID=Msa1420260-mRNA-1:exon:1338;Parent=Msa1420260-mRNA-1
tig0024770 exon 53983 54046 53983 ID=Msa1420260-mRNA-1:exon:1339;Parent=Msa1420260-mRNA-1
tig0024770 exon 56625 56670 56625 ID=Msa1420260-mRNA-1:exon:1340;Parent=Msa1420260-mRNA-1
tig0024770 exon 56870 57298 56870 ID=Msa1420260-mRNA-1:exon:1341;Parent=Msa1420260-mRNA-1
tig0024770 five_prime_UTR 53424 53571 53424 ID=Msa1420260-mRNA-1:five_prime_utr;Parent=Msa1420260-mRNA-1
tig0024770 CDS 53572 53751 53572 ID=Msa1420260-mRNA-1:cds;Parent=Msa1420260-mRNA-1
tig0024770 three_prime_UTR 53752 53897 53752 ID=Msa1420260-mRNA-1:three_prime_utr;Parent=Msa1420260-mRNA-1
tig0024770 three_prime_UTR 53983 54046 53983 ID=Msa1420260-mRNA-1:three_prime_utr;Parent=Msa1420260-mRNA-1
tig0024770 three_prime_UTR 56625 56670 56625 ID=Msa1420260-mRNA-1:three_prime_utr;Parent=Msa1420260-mRNA-1
tig0024770 three_prime_UTR 56870 57298 56870 ID=Msa1420260-mRNA-1:three_prime_utr;Parent=Msa1420260-mRNA-1
Gene Sequence

>Msa1420260

ATGACACTTGGTTTGATCAATGCTAATCCTATAGTCCACGCCAAAAAAGAACGAATTCCTCGCTCCGAAGAACCTCACTCTGATCATTTCGTTGATCCTCTCGATATTTATGATATCCTTCTTTCATTTTTTTTTTATTTCTCTTCATTTATATATTTTCTCTATGTTTACTTGAATTGA
Protein sequence

>Msa1420260

MTLGLINANPIVHAKKERIPRSEEPHSDHFVDPLDIYDILLSFFFYFSSFIYFLYVYLN*