Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0690220 | XP_013456587.1 | 99.231 | 260 | 2 | 0 | 1 | 260 | 1 | 260 | 0.0 | 536 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0690220 | sp|Q9LZT4|EXLA1_ARATH | 62.452 | 261 | 95 | 2 | 3 | 260 | 5 | 265 | 1.25e-111 | 325 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0690220 | I3SMJ2 | 99.231 | 260 | 2 | 0 | 1 | 260 | 1 | 260 | 0.0 | 536 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0607170 | Msa0690220 | 0.917314 | 6.177190e-86 | -8.615850e-47 |
| Msa0647350 | Msa0690220 | 0.854647 | 1.070972e-61 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0690220 | MtrunA17_Chr4g0038741 | 99.231 | 260 | 2 | 0 | 1 | 260 | 1 | 260 | 0.0 | 536 |
| Msa0690220 | MtrunA17_Chr4g0055061 | 40.079 | 252 | 139 | 6 | 4 | 245 | 5 | 254 | 1.11e-62 | 197 |
| Msa0690220 | MtrunA17_Chr5g0399811 | 39.344 | 244 | 138 | 5 | 4 | 242 | 12 | 250 | 2.30e-56 | 181 |
| Msa0690220 | MtrunA17_Chr4g0055561 | 37.838 | 222 | 134 | 3 | 26 | 244 | 31 | 251 | 1.71e-53 | 173 |
| Msa0690220 | MtrunA17_Chr4g0055601 | 36.123 | 227 | 142 | 2 | 19 | 242 | 24 | 250 | 4.01e-53 | 172 |
| Msa0690220 | MtrunA17_Chr7g0231211 | 33.333 | 213 | 137 | 4 | 26 | 234 | 50 | 261 | 8.26e-36 | 128 |
| Msa0690220 | MtrunA17_Chr2g0324201 | 30.435 | 253 | 161 | 7 | 3 | 244 | 9 | 257 | 1.91e-29 | 111 |
| Msa0690220 | MtrunA17_Chr2g0324221 | 29.688 | 256 | 163 | 7 | 2 | 244 | 8 | 259 | 5.70e-29 | 110 |
| Msa0690220 | MtrunA17_Chr7g0231221 | 31.100 | 209 | 137 | 4 | 40 | 242 | 67 | 274 | 2.35e-27 | 106 |
| Msa0690220 | MtrunA17_Chr4g0012801 | 31.132 | 212 | 136 | 6 | 40 | 246 | 61 | 267 | 4.21e-27 | 105 |
| Msa0690220 | MtrunA17_Chr4g0021641 | 29.008 | 262 | 161 | 7 | 2 | 242 | 6 | 263 | 4.53e-27 | 105 |
| Msa0690220 | MtrunA17_Chr7g0218741 | 30.952 | 252 | 143 | 9 | 5 | 238 | 10 | 248 | 6.17e-22 | 91.7 |
| Msa0690220 | MtrunA17_Chr7g0218751 | 29.528 | 254 | 146 | 10 | 5 | 240 | 10 | 248 | 2.15e-20 | 87.4 |
| Msa0690220 | MtrunA17_Chr3g0092071 | 30.081 | 246 | 137 | 10 | 11 | 237 | 13 | 242 | 3.52e-20 | 86.7 |
| Msa0690220 | MtrunA17_Chr4g0069711 | 27.273 | 231 | 136 | 7 | 25 | 237 | 63 | 279 | 5.93e-19 | 84.0 |
| Msa0690220 | MtrunA17_Chr8g0360041 | 29.070 | 258 | 150 | 9 | 5 | 243 | 11 | 254 | 9.24e-19 | 82.8 |
| Msa0690220 | MtrunA17_Chr1g0193631 | 29.339 | 242 | 132 | 10 | 24 | 242 | 31 | 256 | 2.39e-18 | 82.0 |
| Msa0690220 | MtrunA17_Chr7g0271001 | 31.416 | 226 | 130 | 10 | 32 | 242 | 38 | 253 | 1.69e-17 | 79.3 |
| Msa0690220 | MtrunA17_Chr7g0218761 | 30.734 | 218 | 120 | 8 | 38 | 239 | 6 | 208 | 4.65e-17 | 77.4 |
| Msa0690220 | MtrunA17_Chr8g0360721 | 28.899 | 218 | 124 | 8 | 38 | 239 | 15 | 217 | 7.80e-17 | 77.0 |
| Msa0690220 | MtrunA17_Chr5g0417701 | 29.070 | 258 | 146 | 11 | 1 | 237 | 1 | 242 | 1.63e-16 | 76.6 |
| Msa0690220 | MtrunA17_Chr5g0398271 | 29.075 | 227 | 130 | 6 | 34 | 243 | 50 | 262 | 5.00e-16 | 75.5 |
| Msa0690220 | MtrunA17_Chr4g0057091 | 27.434 | 226 | 133 | 7 | 34 | 242 | 49 | 260 | 1.40e-15 | 74.3 |
| Msa0690220 | MtrunA17_Chr5g0432611 | 30.317 | 221 | 123 | 9 | 34 | 238 | 42 | 247 | 2.30e-15 | 73.6 |
| Msa0690220 | MtrunA17_Chr3g0128791 | 27.602 | 221 | 127 | 9 | 34 | 237 | 44 | 248 | 8.94e-15 | 72.0 |
| Msa0690220 | MtrunA17_Chr3g0128801 | 27.602 | 221 | 127 | 9 | 34 | 237 | 44 | 248 | 1.09e-14 | 71.6 |
| Msa0690220 | MtrunA17_Chr4g0042521 | 27.477 | 222 | 130 | 7 | 32 | 237 | 36 | 242 | 1.40e-14 | 71.2 |
| Msa0690220 | MtrunA17_Chr1g0157061 | 27.111 | 225 | 142 | 9 | 32 | 243 | 26 | 241 | 2.51e-14 | 70.5 |
| Msa0690220 | MtrunA17_Chr7g0238681 | 27.556 | 225 | 128 | 9 | 32 | 237 | 43 | 251 | 3.31e-14 | 70.5 |
| Msa0690220 | MtrunA17_Chr3g0107291 | 25.676 | 222 | 130 | 9 | 34 | 237 | 51 | 255 | 4.65e-14 | 70.1 |
| Msa0690220 | MtrunA17_Chr5g0435311 | 27.556 | 225 | 128 | 10 | 32 | 237 | 93 | 301 | 6.96e-14 | 70.1 |
| Msa0690220 | MtrunA17_Chr4g0055581 | 34.343 | 99 | 63 | 2 | 84 | 180 | 9 | 107 | 1.46e-13 | 65.5 |
| Msa0690220 | MtrunA17_Chr7g0217661 | 25.397 | 252 | 153 | 10 | 1 | 237 | 4 | 235 | 3.50e-13 | 67.4 |
| Msa0690220 | MtrunA17_Chr3g0107311 | 25.225 | 222 | 131 | 9 | 34 | 237 | 45 | 249 | 8.75e-13 | 66.2 |
| Msa0690220 | MtrunA17_Chr5g0433841 | 26.293 | 232 | 137 | 9 | 26 | 238 | 44 | 260 | 9.54e-13 | 66.2 |
| Msa0690220 | MtrunA17_Chr4g0021561 | 25.794 | 252 | 150 | 11 | 4 | 234 | 13 | 248 | 2.47e-12 | 65.1 |
| Msa0690220 | MtrunA17_Chr2g0328261 | 27.027 | 222 | 127 | 9 | 32 | 234 | 45 | 250 | 3.54e-12 | 64.7 |
| Msa0690220 | MtrunA17_Chr4g0031621 | 28.191 | 188 | 104 | 7 | 32 | 201 | 43 | 217 | 2.34e-11 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0690220 | AT3G45970.1 | 62.452 | 261 | 95 | 2 | 3 | 260 | 5 | 265 | 1.27e-112 | 325 |
| Msa0690220 | AT3G45960.2 | 64.567 | 254 | 88 | 1 | 3 | 254 | 5 | 258 | 6.48e-112 | 323 |
| Msa0690220 | AT4G38400.1 | 64.372 | 247 | 86 | 1 | 16 | 260 | 19 | 265 | 8.34e-110 | 318 |
| Msa0690220 | AT3G45970.2 | 61.233 | 227 | 85 | 2 | 37 | 260 | 12 | 238 | 7.62e-107 | 309 |
| Msa0690220 | AT3G45960.1 | 64.286 | 210 | 73 | 1 | 47 | 254 | 1 | 210 | 2.79e-102 | 296 |
| Msa0690220 | AT4G17030.1 | 39.640 | 222 | 132 | 1 | 25 | 244 | 29 | 250 | 4.33e-56 | 180 |
| Msa0690220 | AT1G65680.1 | 34.440 | 241 | 146 | 7 | 11 | 242 | 35 | 272 | 5.71e-32 | 119 |
| Msa0690220 | AT2G20750.1 | 31.321 | 265 | 154 | 9 | 5 | 246 | 11 | 270 | 8.18e-30 | 113 |
| Msa0690220 | AT3G60570.2 | 32.864 | 213 | 132 | 8 | 38 | 244 | 3 | 210 | 1.13e-28 | 108 |
| Msa0690220 | AT3G60570.1 | 32.407 | 216 | 135 | 8 | 33 | 242 | 46 | 256 | 3.11e-28 | 108 |
| Msa0690220 | AT4G28250.1 | 28.352 | 261 | 168 | 7 | 1 | 246 | 7 | 263 | 6.21e-28 | 108 |
| Msa0690220 | AT1G65681.1 | 28.571 | 210 | 142 | 4 | 38 | 242 | 15 | 221 | 5.34e-26 | 102 |
| Msa0690220 | AT2G45110.1 | 30.806 | 211 | 138 | 4 | 38 | 242 | 50 | 258 | 2.02e-25 | 101 |
| Msa0690220 | AT4G28250.2 | 27.586 | 261 | 164 | 8 | 1 | 246 | 7 | 257 | 5.26e-23 | 94.7 |
| Msa0690220 | AT2G20750.2 | 33.333 | 198 | 107 | 7 | 5 | 180 | 11 | 205 | 4.33e-22 | 91.7 |
| Msa0690220 | AT5G39290.1 | 27.935 | 247 | 147 | 11 | 12 | 242 | 47 | 278 | 2.59e-18 | 82.4 |
| Msa0690220 | AT1G20190.1 | 31.646 | 237 | 136 | 11 | 23 | 243 | 26 | 252 | 6.33e-18 | 80.9 |
| Msa0690220 | AT1G12560.1 | 31.579 | 209 | 127 | 8 | 40 | 239 | 56 | 257 | 7.76e-17 | 78.2 |
| Msa0690220 | AT5G39270.1 | 27.126 | 247 | 149 | 11 | 12 | 242 | 47 | 278 | 1.77e-16 | 77.4 |
| Msa0690220 | AT2G03090.1 | 27.600 | 250 | 146 | 10 | 7 | 237 | 14 | 247 | 3.84e-16 | 76.3 |
| Msa0690220 | AT5G05290.1 | 26.587 | 252 | 153 | 8 | 5 | 238 | 14 | 251 | 5.55e-16 | 75.9 |
| Msa0690220 | AT5G39260.1 | 27.273 | 231 | 155 | 7 | 13 | 238 | 35 | 257 | 9.09e-16 | 75.1 |
| Msa0690220 | AT4G28250.3 | 32.877 | 146 | 85 | 5 | 40 | 180 | 55 | 192 | 9.13e-16 | 74.3 |
| Msa0690220 | AT2G40610.1 | 28.507 | 221 | 126 | 9 | 34 | 237 | 43 | 248 | 9.83e-16 | 75.1 |
| Msa0690220 | AT5G39310.1 | 28.899 | 218 | 125 | 9 | 40 | 242 | 109 | 311 | 1.08e-15 | 75.9 |
| Msa0690220 | AT1G69530.2 | 28.959 | 221 | 124 | 8 | 34 | 237 | 40 | 244 | 2.61e-15 | 73.9 |
| Msa0690220 | AT1G69530.1 | 28.959 | 221 | 124 | 8 | 34 | 237 | 40 | 244 | 2.61e-15 | 73.9 |
| Msa0690220 | AT4G28250.4 | 27.979 | 193 | 117 | 6 | 1 | 179 | 7 | 191 | 2.89e-15 | 72.8 |
| Msa0690220 | AT2G39700.1 | 28.444 | 225 | 126 | 9 | 32 | 237 | 42 | 250 | 2.90e-15 | 73.9 |
| Msa0690220 | AT1G69530.3 | 28.571 | 224 | 127 | 8 | 34 | 240 | 40 | 247 | 3.44e-15 | 73.9 |
| Msa0690220 | AT5G02260.1 | 27.350 | 234 | 133 | 11 | 25 | 237 | 34 | 251 | 8.17e-15 | 72.4 |
| Msa0690220 | AT1G26770.1 | 25.692 | 253 | 153 | 8 | 1 | 234 | 4 | 240 | 1.50e-14 | 71.6 |
| Msa0690220 | AT1G26770.2 | 25.296 | 253 | 154 | 7 | 1 | 234 | 14 | 250 | 1.51e-14 | 71.6 |
| Msa0690220 | AT4G01630.1 | 27.413 | 259 | 160 | 11 | 1 | 243 | 9 | 255 | 1.83e-14 | 71.6 |
| Msa0690220 | AT5G56320.1 | 26.293 | 232 | 136 | 10 | 25 | 237 | 31 | 246 | 3.41e-14 | 70.9 |
| Msa0690220 | AT5G56320.2 | 26.293 | 232 | 136 | 10 | 25 | 237 | 31 | 246 | 3.41e-14 | 70.9 |
| Msa0690220 | AT5G56320.3 | 26.293 | 232 | 136 | 10 | 25 | 237 | 31 | 246 | 4.55e-14 | 70.9 |
| Msa0690220 | AT2G37640.1 | 27.523 | 218 | 137 | 7 | 32 | 237 | 47 | 255 | 1.81e-13 | 68.9 |
| Msa0690220 | AT1G69530.4 | 30.387 | 181 | 97 | 6 | 34 | 198 | 40 | 207 | 2.51e-13 | 67.8 |
| Msa0690220 | AT1G69530.5 | 30.387 | 181 | 97 | 6 | 34 | 198 | 40 | 207 | 3.29e-13 | 67.8 |
| Msa0690220 | AT1G20190.2 | 31.000 | 200 | 114 | 10 | 57 | 242 | 3 | 192 | 4.36e-13 | 66.6 |
| Msa0690220 | AT5G39280.1 | 25.820 | 244 | 150 | 9 | 15 | 242 | 46 | 274 | 4.88e-13 | 67.8 |
| Msa0690220 | AT3G15370.1 | 28.926 | 242 | 141 | 10 | 15 | 239 | 19 | 246 | 5.09e-13 | 67.4 |
| Msa0690220 | AT2G37640.2 | 27.230 | 213 | 134 | 7 | 37 | 237 | 27 | 230 | 1.02e-12 | 66.2 |
| Msa0690220 | AT5G39300.1 | 25.101 | 247 | 154 | 9 | 12 | 242 | 44 | 275 | 1.69e-12 | 66.2 |
| Msa0690220 | AT4G38210.1 | 27.928 | 222 | 127 | 8 | 34 | 237 | 43 | 249 | 2.44e-12 | 65.5 |
| Msa0690220 | AT3G15370.2 | 30.233 | 215 | 121 | 9 | 40 | 239 | 76 | 276 | 2.81e-12 | 65.9 |
| Msa0690220 | AT2G28950.1 | 28.000 | 225 | 127 | 10 | 32 | 237 | 42 | 250 | 1.26e-11 | 63.5 |
| Msa0690220 | AT3G55500.1 | 26.201 | 229 | 126 | 10 | 32 | 237 | 45 | 253 | 2.05e-11 | 62.8 |
| Msa0690220 | AT1G62980.1 | 28.378 | 222 | 128 | 8 | 35 | 239 | 45 | 252 | 2.49e-11 | 62.8 |
| Msa0690220 | AT3G29030.1 | 27.700 | 213 | 130 | 7 | 34 | 237 | 51 | 248 | 4.80e-11 | 62.0 |
Find 62 sgRNAs with CRISPR-Local
Find 124 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGCTATTCCATATGATTTC+TGG | 0.118772 | 4_4:-57837842 | None:intergenic |
| TTTATTCTTCTGTGTATTAT+AGG | 0.146546 | 4_4:+57837798 | Msa0690220:three_prime_UTR |
| TGGTTGTGGTGCTTGCTTTC+AGG | 0.151650 | 4_4:+57836318 | Msa0690220:CDS |
| TCAAAATCATATACCATATA+TGG | 0.235563 | 4_4:+57836006 | Msa0690220:exon |
| GTTTCTTCACTCTTCTATAA+TGG | 0.287049 | 4_4:+57836292 | Msa0690220:CDS |
| ACTAGGTTGGATCTTCAAAT+TGG | 0.295466 | 4_4:+57837475 | Msa0690220:intron |
| AATTTAATTGCTAAATAATC+TGG | 0.300940 | 4_4:-57837289 | None:intergenic |
| ATGAAACATGGTCATAATTC+AGG | 0.316784 | 4_4:+57837709 | Msa0690220:exon |
| CATCAGGGTATGATGGTAAA+TGG | 0.338953 | 4_4:+57837580 | Msa0690220:CDS |
| TGATTTCTGGGTATTACATT+TGG | 0.343681 | 4_4:-57837829 | None:intergenic |
| CAAAGAAAGAAAGCCATATA+TGG | 0.357423 | 4_4:-57836019 | None:intergenic |
| GCAGCTGGGGCATGTGGATA+TGG | 0.360150 | 4_4:+57836226 | Msa0690220:intron |
| GGCATTGCAATTTCGTTTAG+TGG | 0.362944 | 4_4:+57837554 | Msa0690220:CDS |
| AAAAGGTTCTTCCAGCTGAT+TGG | 0.372823 | 4_4:+57837613 | Msa0690220:CDS |
| CTTTACAGCTATGGCTCAAA+AGG | 0.376515 | 4_4:+57836668 | Msa0690220:CDS |
| TCTTTAGCACTTGACTTTAG+TGG | 0.377568 | 4_4:+57836250 | Msa0690220:CDS |
| TTTGGAGAAATAAGAAGCTT+TGG | 0.378209 | 4_4:-57836101 | None:intergenic |
| TTTCGTTTAGTGGTTACATC+AGG | 0.387420 | 4_4:+57837564 | Msa0690220:CDS |
| AGCTATTCCATATGATTTCT+GGG | 0.389705 | 4_4:-57837841 | None:intergenic |
| TTCTATAATGGAGCTGGTTG+TGG | 0.406537 | 4_4:+57836304 | Msa0690220:CDS |
| TGTAATACCCAGAAATCATA+TGG | 0.413943 | 4_4:+57837834 | Msa0690220:three_prime_UTR |
| TATATATGTATATGTTATGT+GGG | 0.430100 | 4_4:-57837756 | None:intergenic |
| TCACTCTTCTATAATGGAGC+TGG | 0.434156 | 4_4:+57836298 | Msa0690220:CDS |
| ACATGAAAGAGCAGAAGCTT+TGG | 0.441652 | 4_4:-57836119 | None:intergenic |
| CTATATATGTATATGTTATG+TGG | 0.474790 | 4_4:-57837757 | None:intergenic |
| CTGTAAAGGCTCTACTGCTT+AGG | 0.481361 | 4_4:-57836653 | None:intergenic |
| AGTGGTGGACACCTTGCAGC+TGG | 0.482221 | 4_4:+57836268 | Msa0690220:CDS |
| GGTATGATGGTAAATGGCTA+TGG | 0.487295 | 4_4:+57837586 | Msa0690220:CDS |
| AGTTTCTTGAGCAGAAATCA+TGG | 0.495684 | 4_4:+57837498 | Msa0690220:CDS |
| GTGGTTACATCAGGGTATGA+TGG | 0.498564 | 4_4:+57837573 | Msa0690220:CDS |
| GGGCAGTGTGGGACACAAGT+AGG | 0.499790 | 4_4:+57837520 | Msa0690220:CDS |
| ATGAAGTTGAGTAGTTACAA+AGG | 0.504732 | 4_4:-57837891 | None:intergenic |
| CAGTAGAGCCTTTACAGCTA+TGG | 0.510486 | 4_4:+57836659 | Msa0690220:CDS |
| GACCATGTTTCATCATTACA+TGG | 0.510819 | 4_4:-57837699 | None:intergenic |
| GTTTCTTGAGCAGAAATCAT+GGG | 0.515717 | 4_4:+57837499 | Msa0690220:CDS |
| AGTAGGTGTTGATGTAGCTC+AGG | 0.516706 | 4_4:+57837339 | Msa0690220:CDS |
| CAGAAATCATGGGGCAGTGT+GGG | 0.519402 | 4_4:+57837509 | Msa0690220:CDS |
| CAAGCTATATGCACAAAAGA+AGG | 0.520775 | 4_4:+57836579 | Msa0690220:CDS |
| TGCCAAGTTTCAAAATCTGT+TGG | 0.529827 | 4_4:-57836698 | None:intergenic |
| AAGATCAGTCAATACCACTT+TGG | 0.552511 | 4_4:-57836604 | None:intergenic |
| GGCAGTGTGGGACACAAGTA+GGG | 0.554581 | 4_4:+57837521 | Msa0690220:CDS |
| TTATATGCAGCTGGGGCATG+TGG | 0.560990 | 4_4:+57836220 | Msa0690220:intron |
| CACAAGTAGGGTTCCACAAG+GGG | 0.563760 | 4_4:+57837533 | Msa0690220:CDS |
| TTAGCACTTGACTTTAGTGG+TGG | 0.565026 | 4_4:+57836253 | Msa0690220:CDS |
| TTCGTTTAGTGGTTACATCA+GGG | 0.571759 | 4_4:+57837565 | Msa0690220:CDS |
| GATTACAGAAATTGCACAGG+AGG | 0.581799 | 4_4:+57837668 | Msa0690220:CDS |
| TCAGATTACAGAAATTGCAC+AGG | 0.586054 | 4_4:+57837665 | Msa0690220:CDS |
| CGAAATTGCAATGCCCCTTG+TGG | 0.586600 | 4_4:-57837546 | None:intergenic |
| GTATGATGGTAAATGGCTAT+GGG | 0.593985 | 4_4:+57837587 | Msa0690220:CDS |
| TTGTTGACATTGAATACAAG+AGG | 0.607511 | 4_4:+57836721 | Msa0690220:CDS |
| GTGAAGAAACACCAGCTGCA+AGG | 0.611075 | 4_4:-57836279 | None:intergenic |
| TTTACAGCTATGGCTCAAAA+GGG | 0.611980 | 4_4:+57836669 | Msa0690220:CDS |
| ACACAAGTAGGGTTCCACAA+GGG | 0.618151 | 4_4:+57837532 | Msa0690220:CDS |
| CAAGCAACGATCACAAGCAG+TGG | 0.634417 | 4_4:-57836071 | None:intergenic |
| GCAGAAATCATGGGGCAGTG+TGG | 0.638652 | 4_4:+57837508 | Msa0690220:CDS |
| ATTACAGAAATTGCACAGGA+GGG | 0.647308 | 4_4:+57837669 | Msa0690220:CDS |
| TTTCTTGAGCAGAAATCATG+GGG | 0.654832 | 4_4:+57837500 | Msa0690220:CDS |
| AGTACAAAACAATTTGACAA+AGG | 0.655056 | 4_4:-57835968 | None:intergenic |
| GACACAAGTAGGGTTCCACA+AGG | 0.655516 | 4_4:+57837531 | Msa0690220:CDS |
| CACAAAAGAAGGTACCAAAG+TGG | 0.665562 | 4_4:+57836590 | Msa0690220:CDS |
| ATATATGTATATGTTATGTG+GGG | 0.681513 | 4_4:-57837755 | None:intergenic |
| CACCATGTAATGATGAAACA+TGG | 0.686342 | 4_4:+57837697 | Msa0690220:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAAAAAATATATTTAATT+TGG | - | chr4_4:57836494-57836513 | None:intergenic | 0.0% |
| !! | TTTATTTATCAATTTGTATA+TGG | + | chr4_4:57837162-57837181 | Msa0690220:intron | 10.0% |
| !! | TTAAAAAAATAATTAACCAT+TGG | + | chr4_4:57837430-57837449 | Msa0690220:intron | 10.0% |
| !!! | ATTTTATAATGTCGTAAATT+TGG | + | chr4_4:57837106-57837125 | Msa0690220:intron | 15.0% |
| !! | AATTTAATTGCTAAATAATC+TGG | - | chr4_4:57837292-57837311 | None:intergenic | 15.0% |
| !!! | CAATTAAATTTTTGTATCAA+GGG | + | chr4_4:57837302-57837321 | Msa0690220:CDS | 15.0% |
| !!! | AATTAAATTTTTGTATCAAG+GGG | + | chr4_4:57837303-57837322 | Msa0690220:CDS | 15.0% |
| !! | TTTAATTCAATAAAAACACT+TGG | - | chr4_4:57837415-57837434 | None:intergenic | 15.0% |
| !! | TATATATGTATATGTTATGT+GGG | - | chr4_4:57837759-57837778 | None:intergenic | 15.0% |
| !!! | CATATACATATATAGATTTT+CGG | + | chr4_4:57837765-57837784 | Msa0690220:three_prime_UTR | 15.0% |
| !! | TCAAAATCATATACCATATA+TGG | + | chr4_4:57836006-57836025 | Msa0690220:exon | 20.0% |
| !!! | TTCATATAATTTGTCTACAT+TGG | - | chr4_4:57836938-57836957 | None:intergenic | 20.0% |
| !!! | GCAATTAAATTTTTGTATCA+AGG | + | chr4_4:57837301-57837320 | Msa0690220:CDS | 20.0% |
| !!! | ATTAAATTTTTGTATCAAGG+GGG | + | chr4_4:57837304-57837323 | Msa0690220:CDS | 20.0% |
| !! | ATATATGTATATGTTATGTG+GGG | - | chr4_4:57837758-57837777 | None:intergenic | 20.0% |
| !! | CTATATATGTATATGTTATG+TGG | - | chr4_4:57837760-57837779 | None:intergenic | 20.0% |
| !! | TTTATTCTTCTGTGTATTAT+AGG | + | chr4_4:57837798-57837817 | Msa0690220:three_prime_UTR | 20.0% |
| ! | AGTACAAAACAATTTGACAA+AGG | - | chr4_4:57835971-57835990 | None:intergenic | 25.0% |
| !!! | ATGATTTTGAGAAATGAGAA+GGG | - | chr4_4:57835996-57836015 | None:intergenic | 25.0% |
| !!! | TATGATTTTGAGAAATGAGA+AGG | - | chr4_4:57835997-57836016 | None:intergenic | 25.0% |
| !!! | TGATTTTTTTTATATGCAGC+TGG | + | chr4_4:57836211-57836230 | Msa0690220:intron | 25.0% |
| !!! | GATTTTTTTTATATGCAGCT+GGG | + | chr4_4:57836212-57836231 | Msa0690220:intron | 25.0% |
| !!! | ATTTTTTTTATATGCAGCTG+GGG | + | chr4_4:57836213-57836232 | Msa0690220:intron | 25.0% |
| !!! | ATTTAATTTGGCTAAATGTC+AGG | - | chr4_4:57836482-57836501 | None:intergenic | 25.0% |
| ! | ATTAATGAACAATTTGATGC+AGG | + | chr4_4:57836542-57836561 | Msa0690220:intron | 25.0% |
| ! | TAGCGCAATAATAATAGTAA+CGG | + | chr4_4:57836974-57836993 | Msa0690220:intron | 25.0% |
| ! | TAATGTCGTAAATTTGGAAA+TGG | + | chr4_4:57837112-57837131 | Msa0690220:intron | 25.0% |
| ! | TGAGTACAAAAAACAGAATT+TGG | + | chr4_4:57837237-57837256 | Msa0690220:CDS | 25.0% |
| ! | CTTATTAATTACTACTAGGT+TGG | + | chr4_4:57837462-57837481 | Msa0690220:intron | 25.0% |
| !! | ATACATATATAGATTTTCGG+TGG | + | chr4_4:57837768-57837787 | Msa0690220:three_prime_UTR | 25.0% |
| CAAAGAAAGAAAGCCATATA+TGG | - | chr4_4:57836022-57836041 | None:intergenic | 30.0% | |
| TTTGGAGAAATAAGAAGCTT+TGG | - | chr4_4:57836104-57836123 | None:intergenic | 30.0% | |
| GTTTCTTCACTCTTCTATAA+TGG | + | chr4_4:57836292-57836311 | Msa0690220:CDS | 30.0% | |
| !!! | GTCAGGATAGTTTTAAAATG+CGG | - | chr4_4:57836465-57836484 | None:intergenic | 30.0% |
| TTGTTGACATTGAATACAAG+AGG | + | chr4_4:57836721-57836740 | Msa0690220:CDS | 30.0% | |
| GAAGTAAAGCTACAAAAAAG+TGG | - | chr4_4:57836760-57836779 | None:intergenic | 30.0% | |
| !! | CATTGGTGTTCTATTTTCAA+TGG | - | chr4_4:57836921-57836940 | None:intergenic | 30.0% |
| !!! | TTCTGTTTTTTGTACTCACA+TGG | - | chr4_4:57837235-57837254 | None:intergenic | 30.0% |
| !!! | CACTTGGTAATGATTTTTGT+TGG | - | chr4_4:57837399-57837418 | None:intergenic | 30.0% |
| !! | TAATTAACCATTGGTTTCTG+TGG | + | chr4_4:57837439-57837458 | Msa0690220:intron | 30.0% |
| GTGGCTTATTAATTACTACT+AGG | + | chr4_4:57837458-57837477 | Msa0690220:intron | 30.0% | |
| ATGAAACATGGTCATAATTC+AGG | + | chr4_4:57837709-57837728 | Msa0690220:exon | 30.0% | |
| ! | TGATTTCTGGGTATTACATT+TGG | - | chr4_4:57837832-57837851 | None:intergenic | 30.0% |
| TGTAATACCCAGAAATCATA+TGG | + | chr4_4:57837834-57837853 | Msa0690220:three_prime_UTR | 30.0% | |
| AGCTATTCCATATGATTTCT+GGG | - | chr4_4:57837844-57837863 | None:intergenic | 30.0% | |
| AAGCTATTCCATATGATTTC+TGG | - | chr4_4:57837845-57837864 | None:intergenic | 30.0% | |
| ATGAAGTTGAGTAGTTACAA+AGG | - | chr4_4:57837894-57837913 | None:intergenic | 30.0% | |
| TCTTTAGCACTTGACTTTAG+TGG | + | chr4_4:57836250-57836269 | Msa0690220:CDS | 35.0% | |
| ATGATTAATAACACCGGCAA+CGG | - | chr4_4:57836395-57836414 | None:intergenic | 35.0% | |
| ! | CTTCTTTTGTGCATATAGCT+TGG | - | chr4_4:57836581-57836600 | None:intergenic | 35.0% |
| CAAGCTATATGCACAAAAGA+AGG | + | chr4_4:57836579-57836598 | Msa0690220:CDS | 35.0% | |
| AAGATCAGTCAATACCACTT+TGG | - | chr4_4:57836607-57836626 | None:intergenic | 35.0% | |
| TTTACAGCTATGGCTCAAAA+GGG | + | chr4_4:57836669-57836688 | Msa0690220:CDS | 35.0% | |
| !!! | AGCCAACAGATTTTGAAACT+TGG | + | chr4_4:57836696-57836715 | Msa0690220:CDS | 35.0% |
| TGCCAAGTTTCAAAATCTGT+TGG | - | chr4_4:57836701-57836720 | None:intergenic | 35.0% | |
| ! | TCTTGATATGCCAGCATTTT+TGG | + | chr4_4:57836849-57836868 | Msa0690220:intron | 35.0% |
| !! | TTCTATTTTCAATGGTGGGA+CGG | - | chr4_4:57836913-57836932 | None:intergenic | 35.0% |
| ! | GGTGTTCTATTTTCAATGGT+GGG | - | chr4_4:57836917-57836936 | None:intergenic | 35.0% |
| ! | TGGTGTTCTATTTTCAATGG+TGG | - | chr4_4:57836918-57836937 | None:intergenic | 35.0% |
| ! | TAATCCCACGTGGATTTTAA+TGG | + | chr4_4:57837200-57837219 | Msa0690220:intron | 35.0% |
| TAATAAGCCACAGAAACCAA+TGG | - | chr4_4:57837449-57837468 | None:intergenic | 35.0% | |
| ! | ACTAGGTTGGATCTTCAAAT+TGG | + | chr4_4:57837475-57837494 | Msa0690220:intron | 35.0% |
| AGTTTCTTGAGCAGAAATCA+TGG | + | chr4_4:57837498-57837517 | Msa0690220:CDS | 35.0% | |
| GTTTCTTGAGCAGAAATCAT+GGG | + | chr4_4:57837499-57837518 | Msa0690220:CDS | 35.0% | |
| TTTCTTGAGCAGAAATCATG+GGG | + | chr4_4:57837500-57837519 | Msa0690220:CDS | 35.0% | |
| TTTCGTTTAGTGGTTACATC+AGG | + | chr4_4:57837564-57837583 | Msa0690220:CDS | 35.0% | |
| TTCGTTTAGTGGTTACATCA+GGG | + | chr4_4:57837565-57837584 | Msa0690220:CDS | 35.0% | |
| GTATGATGGTAAATGGCTAT+GGG | + | chr4_4:57837587-57837606 | Msa0690220:CDS | 35.0% | |
| TAAATGGCTATGGGCAAAAA+AGG | + | chr4_4:57837596-57837615 | Msa0690220:CDS | 35.0% | |
| TTCCAGCTGATTGGAAAAAT+GGG | + | chr4_4:57837622-57837641 | Msa0690220:CDS | 35.0% | |
| TCAGATTACAGAAATTGCAC+AGG | + | chr4_4:57837665-57837684 | Msa0690220:CDS | 35.0% | |
| ATTACAGAAATTGCACAGGA+GGG | + | chr4_4:57837669-57837688 | Msa0690220:CDS | 35.0% | |
| CACCATGTAATGATGAAACA+TGG | + | chr4_4:57837697-57837716 | Msa0690220:CDS | 35.0% | |
| GACCATGTTTCATCATTACA+TGG | - | chr4_4:57837702-57837721 | None:intergenic | 35.0% | |
| ACATGAAAGAGCAGAAGCTT+TGG | - | chr4_4:57836122-57836141 | None:intergenic | 40.0% | |
| TTAGCACTTGACTTTAGTGG+TGG | + | chr4_4:57836253-57836272 | Msa0690220:CDS | 40.0% | |
| ! | TCACTCTTCTATAATGGAGC+TGG | + | chr4_4:57836298-57836317 | Msa0690220:CDS | 40.0% |
| ! | TTCTATAATGGAGCTGGTTG+TGG | + | chr4_4:57836304-57836323 | Msa0690220:CDS | 40.0% |
| GAGCACAAATCATCGTATTC+AGG | + | chr4_4:57836344-57836363 | Msa0690220:intron | 40.0% | |
| !! | TGCCGGTGTTATTAATCATG+CGG | + | chr4_4:57836396-57836415 | Msa0690220:intron | 40.0% |
| GTCCGCATGATTAATAACAC+CGG | - | chr4_4:57836401-57836420 | None:intergenic | 40.0% | |
| !! | CACAAAAGAAGGTACCAAAG+TGG | + | chr4_4:57836590-57836609 | Msa0690220:CDS | 40.0% |
| ! | CTTTTGAGCCATAGCTGTAA+AGG | - | chr4_4:57836670-57836689 | None:intergenic | 40.0% |
| CTTTACAGCTATGGCTCAAA+AGG | + | chr4_4:57836668-57836687 | Msa0690220:CDS | 40.0% | |
| ATGCTGGCATATCAAGAATG+TGG | - | chr4_4:57836846-57836865 | None:intergenic | 40.0% | |
| !!! | GATATGCCAGCATTTTTGGT+TGG | + | chr4_4:57836853-57836872 | Msa0690220:intron | 40.0% |
| ! | GAACATTGATTAATCCCACG+TGG | + | chr4_4:57837190-57837209 | Msa0690220:intron | 40.0% |
| TGCACCATTAAAATCCACGT+GGG | - | chr4_4:57837207-57837226 | None:intergenic | 40.0% | |
| GGCATTGCAATTTCGTTTAG+TGG | + | chr4_4:57837554-57837573 | Msa0690220:CDS | 40.0% | |
| CATCAGGGTATGATGGTAAA+TGG | + | chr4_4:57837580-57837599 | Msa0690220:CDS | 40.0% | |
| GGTATGATGGTAAATGGCTA+TGG | + | chr4_4:57837586-57837605 | Msa0690220:CDS | 40.0% | |
| AAAAGGTTCTTCCAGCTGAT+TGG | + | chr4_4:57837613-57837632 | Msa0690220:CDS | 40.0% | |
| CTTCCAGCTGATTGGAAAAA+TGG | + | chr4_4:57837621-57837640 | Msa0690220:CDS | 40.0% | |
| TCCAGCTGATTGGAAAAATG+GGG | + | chr4_4:57837623-57837642 | Msa0690220:CDS | 40.0% | |
| GATTACAGAAATTGCACAGG+AGG | + | chr4_4:57837668-57837687 | Msa0690220:CDS | 40.0% | |
| CTGTAAAGGCTCTACTGCTT+AGG | - | chr4_4:57836656-57836675 | None:intergenic | 45.0% | |
| CAGTAGAGCCTTTACAGCTA+TGG | + | chr4_4:57836659-57836678 | Msa0690220:CDS | 45.0% | |
| ! | TACTAGCTTAGGTGGGAAGT+GGG | - | chr4_4:57836880-57836899 | None:intergenic | 45.0% |
| ! | CACTGTCTACTAGCTTAGGT+GGG | - | chr4_4:57836887-57836906 | None:intergenic | 45.0% |
| CTGCACCATTAAAATCCACG+TGG | - | chr4_4:57837208-57837227 | None:intergenic | 45.0% | |
| !! | AGTAGGTGTTGATGTAGCTC+AGG | + | chr4_4:57837339-57837358 | Msa0690220:CDS | 45.0% |
| ACACAAGTAGGGTTCCACAA+GGG | + | chr4_4:57837532-57837551 | Msa0690220:CDS | 45.0% | |
| GTGGTTACATCAGGGTATGA+TGG | + | chr4_4:57837573-57837592 | Msa0690220:CDS | 45.0% | |
| !! | ACCCCATTTTTCCAATCAGC+TGG | - | chr4_4:57837627-57837646 | None:intergenic | 45.0% |
| CAAGCAACGATCACAAGCAG+TGG | - | chr4_4:57836074-57836093 | None:intergenic | 50.0% | |
| TTATATGCAGCTGGGGCATG+TGG | + | chr4_4:57836220-57836239 | Msa0690220:intron | 50.0% | |
| GTGAAGAAACACCAGCTGCA+AGG | - | chr4_4:57836282-57836301 | None:intergenic | 50.0% | |
| TGGTTGTGGTGCTTGCTTTC+AGG | + | chr4_4:57836318-57836337 | Msa0690220:CDS | 50.0% | |
| GTGGGTCCAACCAAAAATGC+TGG | - | chr4_4:57836862-57836881 | None:intergenic | 50.0% | |
| ! | CTACTAGCTTAGGTGGGAAG+TGG | - | chr4_4:57836881-57836900 | None:intergenic | 50.0% |
| ! | CCACTGTCTACTAGCTTAGG+TGG | - | chr4_4:57836888-57836907 | None:intergenic | 50.0% |
| CCACCTAAGCTAGTAGACAG+TGG | + | chr4_4:57836885-57836904 | Msa0690220:intron | 50.0% | |
| ! | GTGCCACTGTCTACTAGCTT+AGG | - | chr4_4:57836891-57836910 | None:intergenic | 50.0% |
| GGGGGTCAAACAGAGATAGT+AGG | + | chr4_4:57837322-57837341 | Msa0690220:CDS | 50.0% | |
| CAGAAATCATGGGGCAGTGT+GGG | + | chr4_4:57837509-57837528 | Msa0690220:CDS | 50.0% | |
| GACACAAGTAGGGTTCCACA+AGG | + | chr4_4:57837531-57837550 | Msa0690220:CDS | 50.0% | |
| CACAAGTAGGGTTCCACAAG+GGG | + | chr4_4:57837533-57837552 | Msa0690220:CDS | 50.0% | |
| CGAAATTGCAATGCCCCTTG+TGG | - | chr4_4:57837549-57837568 | None:intergenic | 50.0% | |
| GTTGCAGTCATTGCCGTTGC+CGG | + | chr4_4:57836379-57836398 | Msa0690220:intron | 55.0% | |
| GCAGAAATCATGGGGCAGTG+TGG | + | chr4_4:57837508-57837527 | Msa0690220:CDS | 55.0% | |
| GGCAGTGTGGGACACAAGTA+GGG | + | chr4_4:57837521-57837540 | Msa0690220:CDS | 55.0% | |
| GCAGCTGGGGCATGTGGATA+TGG | + | chr4_4:57836226-57836245 | Msa0690220:intron | 60.0% | |
| !! | AGTGGTGGACACCTTGCAGC+TGG | + | chr4_4:57836268-57836287 | Msa0690220:CDS | 60.0% |
| GGGCAGTGTGGGACACAAGT+AGG | + | chr4_4:57837520-57837539 | Msa0690220:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_4 | gene | 57835961 | 57837919 | 57835961 | ID=Msa0690220;Name=Msa0690220 |
| chr4_4 | mRNA | 57835961 | 57837919 | 57835961 | ID=Msa0690220-mRNA-1;Parent=Msa0690220;Name=Msa0690220-mRNA-1;_AED=0.15;_eAED=0.15;_QI=64|1|1|1|1|1|5|194|260 |
| chr4_4 | exon | 57835961 | 57836139 | 57835961 | ID=Msa0690220-mRNA-1:exon:15467;Parent=Msa0690220-mRNA-1 |
| chr4_4 | exon | 57836230 | 57836339 | 57836230 | ID=Msa0690220-mRNA-1:exon:15468;Parent=Msa0690220-mRNA-1 |
| chr4_4 | exon | 57836564 | 57836742 | 57836564 | ID=Msa0690220-mRNA-1:exon:15469;Parent=Msa0690220-mRNA-1 |
| chr4_4 | exon | 57837228 | 57837360 | 57837228 | ID=Msa0690220-mRNA-1:exon:15470;Parent=Msa0690220-mRNA-1 |
| chr4_4 | exon | 57837480 | 57837919 | 57837480 | ID=Msa0690220-mRNA-1:exon:15471;Parent=Msa0690220-mRNA-1 |
| chr4_4 | five_prime_UTR | 57835961 | 57836024 | 57835961 | ID=Msa0690220-mRNA-1:five_prime_utr;Parent=Msa0690220-mRNA-1 |
| chr4_4 | CDS | 57836025 | 57836139 | 57836025 | ID=Msa0690220-mRNA-1:cds;Parent=Msa0690220-mRNA-1 |
| chr4_4 | CDS | 57836230 | 57836339 | 57836230 | ID=Msa0690220-mRNA-1:cds;Parent=Msa0690220-mRNA-1 |
| chr4_4 | CDS | 57836564 | 57836742 | 57836564 | ID=Msa0690220-mRNA-1:cds;Parent=Msa0690220-mRNA-1 |
| chr4_4 | CDS | 57837228 | 57837360 | 57837228 | ID=Msa0690220-mRNA-1:cds;Parent=Msa0690220-mRNA-1 |
| chr4_4 | CDS | 57837480 | 57837725 | 57837480 | ID=Msa0690220-mRNA-1:cds;Parent=Msa0690220-mRNA-1 |
| chr4_4 | three_prime_UTR | 57837726 | 57837919 | 57837726 | ID=Msa0690220-mRNA-1:three_prime_utr;Parent=Msa0690220-mRNA-1 |
| Gene Sequence |
| Protein sequence |
