AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0698590


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0698590 MtrunA17_Chr4g0052011 99.153 118 1 0 1 118 35 152 1.04e-83 241
Msa0698590 MtrunA17_Chr8g0370431 46.067 89 48 0 26 114 33 121 3.69e-24 90.1
Msa0698590 MtrunA17_Chr3g0143991 47.674 86 45 0 20 105 35 120 1.59e-23 88.6
Msa0698590 MtrunA17_Chr7g0242251 51.316 76 37 0 26 101 47 122 5.80e-23 87.8
Msa0698590 MtrunA17_Chr1g0212341 48.315 89 45 1 26 113 98 186 8.98e-22 85.5
Msa0698590 MtrunA17_Chr5g0407011 38.272 81 50 0 19 99 25 105 1.98e-15 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0698590 AT2G34450.4 68.085 94 30 0 25 118 58 151 4.40e-45 143
Msa0698590 AT2G34450.3 68.085 94 30 0 25 118 58 151 4.40e-45 143
Msa0698590 AT2G34450.1 68.085 94 30 0 25 118 58 151 4.40e-45 143
Msa0698590 AT2G34450.2 67.368 95 30 1 25 118 58 152 2.92e-43 139
Msa0698590 AT3G51880.4 44.554 101 56 0 17 117 40 140 3.02e-24 91.3
Msa0698590 AT2G17560.3 43.478 115 61 1 8 118 13 127 3.25e-24 90.1
Msa0698590 AT2G17560.2 43.478 115 61 1 8 118 13 127 4.16e-24 90.1
Msa0698590 AT2G17560.1 43.478 115 61 1 8 118 13 127 4.16e-24 90.1
Msa0698590 AT3G51880.5 44.118 102 57 0 17 118 40 141 4.78e-24 90.9
Msa0698590 AT3G51880.1 44.118 102 57 0 17 118 40 141 4.78e-24 90.9
Msa0698590 AT3G51880.3 44.118 102 57 0 17 118 40 141 4.78e-24 90.9
Msa0698590 AT3G51880.2 44.118 102 57 0 17 118 40 141 5.22e-24 91.3
Msa0698590 AT4G35570.1 44.318 88 49 0 26 113 30 117 1.95e-22 85.5
Msa0698590 AT4G35570.2 44.318 88 49 0 26 113 30 117 1.95e-22 85.5
Msa0698590 AT1G20693.3 39.823 113 66 1 3 115 11 121 3.35e-22 85.5
Msa0698590 AT1G20693.1 39.823 113 66 1 3 115 11 121 3.44e-22 85.5
Msa0698590 AT1G20693.2 39.823 113 66 1 3 115 11 121 5.21e-22 84.7
Msa0698590 AT1G20696.1 45.556 90 47 1 26 115 31 118 5.55e-21 82.0
Msa0698590 AT1G20696.5 44.156 77 43 0 26 102 31 107 2.16e-18 75.5
Msa0698590 AT1G20696.2 44.156 77 43 0 26 102 31 107 2.49e-18 75.5
Msa0698590 AT5G23420.1 47.059 68 35 1 27 94 112 178 2.41e-13 64.3
Msa0698590 AT1G20696.4 43.243 74 40 1 42 115 5 76 1.78e-12 59.3
Msa0698590 AT1G20696.3 43.243 74 40 1 42 115 5 76 3.78e-12 58.5

Find 14 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAAATTCTGCACGTTTCT+CGG 0.263566 4_4:-68942762 None:intergenic
GACAGAGTATAACAAGAAAA+TGG 0.358050 4_4:+68942793 Msa0698590:CDS
TTAAGTCTCTTGACTTAGTC+TGG 0.383960 4_4:-68940623 None:intergenic
TCACGCATTGACTTAACATC+TGG 0.420177 4_4:-68941405 None:intergenic
GCAAGGCATGTGGAGAGAAG+TGG 0.571530 4_4:+68941711 Msa0698590:CDS
CGTGTTCATACAGATTGGCA+AGG 0.572075 4_4:+68941694 Msa0698590:intron
AAGAAGTAGAAGAAAGCAGT+GGG 0.576065 4_4:-68940705 None:intergenic
GAAGTAGAAGAAAGCAGTGG+GGG 0.593069 4_4:-68940703 None:intergenic
TCTGATGCTTTGCAGGAAAG+CGG 0.595152 4_4:+68942888 Msa0698590:intron
ATACAGATTGGCAAGGCATG+TGG 0.602187 4_4:+68941701 Msa0698590:intron
CGAATATGAAGAAACTGATG+AGG 0.603289 4_4:+68942911 Msa0698590:CDS
CAAGAAGTAGAAGAAAGCAG+TGG 0.644048 4_4:-68940706 None:intergenic
AAGTAGAAGAAAGCAGTGGG+GGG 0.691336 4_4:-68940702 None:intergenic
AGAAGTAGAAGAAAGCAGTG+GGG 0.730489 4_4:-68940704 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAATTAAGTTTCAAAAAT+TGG + chr4_4:68941843-68941862 Msa0698590:intron 10.0%
!! ATAAATTATGTTTCAAAAAT+TGG + chr4_4:68941918-68941937 Msa0698590:intron 10.0%
!! TTGTTATATTTGAAAAAAAT+TGG + chr4_4:68942075-68942094 Msa0698590:intron 10.0%
!! TAAAAAAAATTAAAGTAAAG+GGG + chr4_4:68942437-68942456 Msa0698590:intron 10.0%
!!! CGATTTTTATGTTTAATTAT+TGG + chr4_4:68942280-68942299 Msa0698590:intron 15.0%
!! AACATCATAAATAAAGAACA+AGG - chr4_4:68940751-68940770 None:intergenic 20.0%
!!! AATTTAGAACAAATGGAAAA+TGG - chr4_4:68940813-68940832 None:intergenic 20.0%
!!! CCATAAAAATTTAGAACAAA+TGG - chr4_4:68940820-68940839 None:intergenic 20.0%
!!! CCATTTGTTCTAAATTTTTA+TGG + chr4_4:68940817-68940836 Msa0698590:intron 20.0%
!! ACTACTATTTACAGTATATT+TGG + chr4_4:68941225-68941244 Msa0698590:intron 20.0%
!!! ACAATTGCTTCTTTTTTATA+TGG + chr4_4:68941289-68941308 Msa0698590:intron 20.0%
!!! ATAACTGGCAATTTTTAAAA+TGG - chr4_4:68941566-68941585 None:intergenic 20.0%
!! TCTCAAAAACATAAAATAAC+TGG - chr4_4:68941581-68941600 None:intergenic 20.0%
!! TTTGAAAAAAATTGGAAAGT+TGG + chr4_4:68942083-68942102 Msa0698590:intron 20.0%
!! TTATGTTTAATTATTGGTGT+TGG + chr4_4:68942286-68942305 Msa0698590:intron 20.0%
!!! AAACTAGTTCTATATTGATT+TGG - chr4_4:68942583-68942602 None:intergenic 20.0%
!! AGATCAAATTGAACATAATA+TGG - chr4_4:68942654-68942673 None:intergenic 20.0%
! ATCATAAATAAAGAACAAGG+AGG - chr4_4:68940748-68940767 None:intergenic 25.0%
! TAGTAATGAACCAGTAATTA+TGG - chr4_4:68940914-68940933 None:intergenic 25.0%
! AATAAGACATAGTAGAAACA+AGG - chr4_4:68941188-68941207 None:intergenic 25.0%
! TTCAAAATTACTGTGTTTAC+TGG + chr4_4:68941327-68941346 Msa0698590:intron 25.0%
!!! AAGTTCTTTTTCATTCTGAA+GGG + chr4_4:68941350-68941369 Msa0698590:intron 25.0%
! TAGACGAAATATTAATACGA+CGG - chr4_4:68941445-68941464 None:intergenic 25.0%
!! AATTCAGGTTAAATATGCAA+GGG - chr4_4:68941777-68941796 None:intergenic 25.0%
!! TAACCTGAATTGTGTTATTA+TGG + chr4_4:68941786-68941805 Msa0698590:intron 25.0%
!! GAATTTTGTCAAAACTGTTT+AGG + chr4_4:68941947-68941966 Msa0698590:intron 25.0%
!!! AACTGTTTAGGTTTTTAATG+TGG + chr4_4:68941959-68941978 Msa0698590:intron 25.0%
!!! ATCAGTTTTTTGACTCATTT+AGG + chr4_4:68942238-68942257 Msa0698590:intron 25.0%
!!! TCAGTTTTTTGACTCATTTA+GGG + chr4_4:68942239-68942258 Msa0698590:intron 25.0%
! ATTATAAAGACACGTAAACT+GGG - chr4_4:68942402-68942421 None:intergenic 25.0%
! TAACAAAAATTGTATCTGAC+AGG + chr4_4:68942712-68942731 Msa0698590:intron 25.0%
AAAAGTAGCACCATAATTAC+TGG + chr4_4:68940901-68940920 Msa0698590:intron 30.0%
!!! GTATTTTACGGATTTTGACT+TGG + chr4_4:68941019-68941038 Msa0698590:intron 30.0%
GCTAATTGCTAATCAACAAA+TGG - chr4_4:68941067-68941086 None:intergenic 30.0%
AAAACATGTTTGAAGATGAG+TGG - chr4_4:68941121-68941140 None:intergenic 30.0%
ATAGTAGTATGATTGAGCAA+AGG - chr4_4:68941213-68941232 None:intergenic 30.0%
TTACAGTATATTTGGCATTG+TGG + chr4_4:68941233-68941252 Msa0698590:intron 30.0%
!! GAAGTTCTTTTTCATTCTGA+AGG + chr4_4:68941349-68941368 Msa0698590:intron 30.0%
TGTAAAACAAAGTTGCTTCA+GGG - chr4_4:68941491-68941510 None:intergenic 30.0%
TTGTAAAACAAAGTTGCTTC+AGG - chr4_4:68941492-68941511 None:intergenic 30.0%
! CAATTCAGGTTAAATATGCA+AGG - chr4_4:68941778-68941797 None:intergenic 30.0%
CAACCATAATAACACAATTC+AGG - chr4_4:68941792-68941811 None:intergenic 30.0%
TTGTCAGTTGTGATATTTGA+CGG + chr4_4:68941877-68941896 Msa0698590:intron 30.0%
! TATGTTTCAAAAATTGGCTC+AGG + chr4_4:68941924-68941943 Msa0698590:intron 30.0%
! ATGTTTCAAAAATTGGCTCA+GGG + chr4_4:68941925-68941944 Msa0698590:intron 30.0%
ATTAACACGAGTACATTTGT+AGG + chr4_4:68942313-68942332 Msa0698590:intron 30.0%
GATTATAAAGACACGTAAAC+TGG - chr4_4:68942403-68942422 None:intergenic 30.0%
!! TGGATGGTATTTTAGAAAAC+TGG - chr4_4:68942470-68942489 None:intergenic 30.0%
!! TTCTAAAATACCATCCATTG+TGG + chr4_4:68942473-68942492 Msa0698590:intron 30.0%
! ATTTGGAATAAATAGCAGCA+CGG - chr4_4:68942566-68942585 None:intergenic 30.0%
GACAGAGTATAACAAGAAAA+TGG + chr4_4:68942793-68942812 Msa0698590:CDS 30.0%
TAACCTATCTGTCATATTCA+TGG + chr4_4:68942816-68942835 Msa0698590:intron 30.0%
TTAAGTCTCTTGACTTAGTC+TGG - chr4_4:68940626-68940645 None:intergenic 35.0%
!!! GTTCTTTTTTTGCACTGCTT+TGG - chr4_4:68940649-68940668 None:intergenic 35.0%
AAGAAGTAGAAGAAAGCAGT+GGG - chr4_4:68940708-68940727 None:intergenic 35.0%
ACAAGAGTTTAGCTGCATTA+AGG + chr4_4:68940857-68940876 Msa0698590:intron 35.0%
ACTGATATGTGTGTTTCCTT+TGG + chr4_4:68940961-68940980 Msa0698590:intron 35.0%
! TATACTATCGCGTAAGTTGA+TGG + chr4_4:68940987-68941006 Msa0698590:intron 35.0%
!! ATACTATCGCGTAAGTTGAT+GGG + chr4_4:68940988-68941007 Msa0698590:intron 35.0%
! TGGGGTTCTGTTGTATTTTA+CGG + chr4_4:68941007-68941026 Msa0698590:intron 35.0%
!!! TTTTACGGATTTTGACTTGG+AGG + chr4_4:68941022-68941041 Msa0698590:intron 35.0%
GACATAGTAGAAACAAGGAA+AGG - chr4_4:68941183-68941202 None:intergenic 35.0%
!! TTCTTTTTCATTCTGAAGGG+AGG + chr4_4:68941353-68941372 Msa0698590:intron 35.0%
GTAAAACAAAGTTGCTTCAG+GGG - chr4_4:68941490-68941509 None:intergenic 35.0%
! GATGATGATGATGATGATGA+TGG - chr4_4:68941616-68941635 None:intergenic 35.0%
! GTGGAAAACAATGACTTTTG+AGG + chr4_4:68941730-68941749 Msa0698590:CDS 35.0%
AAGAAGTCATGATCAAGACA+TGG - chr4_4:68942006-68942025 None:intergenic 35.0%
TGGAAAGTTGGAATTCATTG+CGG + chr4_4:68942095-68942114 Msa0698590:intron 35.0%
CCATTGTCAGGAATTTAAAC+AGG - chr4_4:68942619-68942638 None:intergenic 35.0%
CCTGTTTAAATTCCTGACAA+TGG + chr4_4:68942616-68942635 Msa0698590:intron 35.0%
TCAATTTGATCTGAGCAAGA+AGG + chr4_4:68942662-68942681 Msa0698590:intron 35.0%
ATCAAATTCTGCACGTTTCT+CGG - chr4_4:68942765-68942784 None:intergenic 35.0%
TAGCCATGAATATGACAGAT+AGG - chr4_4:68942822-68942841 None:intergenic 35.0%
! CTAGTGAACTGACTCTTATT+TGG + chr4_4:68942839-68942858 Msa0698590:intron 35.0%
!! TTTGTTTTCTGATGCTTTGC+AGG + chr4_4:68942881-68942900 Msa0698590:intron 35.0%
CGAATATGAAGAAACTGATG+AGG + chr4_4:68942911-68942930 Msa0698590:CDS 35.0%
AGAAGTAGAAGAAAGCAGTG+GGG - chr4_4:68940707-68940726 None:intergenic 40.0%
CAAGAAGTAGAAGAAAGCAG+TGG - chr4_4:68940709-68940728 None:intergenic 40.0%
ACGCGATAGTATAACTCCAA+AGG - chr4_4:68940980-68940999 None:intergenic 40.0%
!! TACTATCGCGTAAGTTGATG+GGG + chr4_4:68940989-68941008 Msa0698590:intron 40.0%
!! CATTCTGAAGGGAGGATTTT+CGG + chr4_4:68941361-68941380 Msa0698590:intron 40.0%
!! TCACGCATTGACTTAACATC+TGG - chr4_4:68941408-68941427 None:intergenic 40.0%
CAATCTGTATGAACACGGAA+AGG - chr4_4:68941691-68941710 None:intergenic 40.0%
CTTTCCGTGTTCATACAGAT+TGG + chr4_4:68941689-68941708 Msa0698590:intron 40.0%
CTTGCCAATCTGTATGAACA+CGG - chr4_4:68941696-68941715 None:intergenic 40.0%
AGCAGAACTGAAGATCACTT+TGG + chr4_4:68942120-68942139 Msa0698590:intron 40.0%
AGGTAAGTTTCCACAATGGA+TGG - chr4_4:68942486-68942505 None:intergenic 40.0%
ATCCATTGTGGAAACTTACC+TGG + chr4_4:68942485-68942504 Msa0698590:intron 40.0%
TCCATTGTGGAAACTTACCT+GGG + chr4_4:68942486-68942505 Msa0698590:intron 40.0%
ATACTTGACACTCCATTGTC+AGG - chr4_4:68942631-68942650 None:intergenic 40.0%
AACATAGAGCTCAACCAAAG+CGG - chr4_4:68942693-68942712 None:intergenic 40.0%
AAGTAGAAGAAAGCAGTGGG+GGG - chr4_4:68940705-68940724 None:intergenic 45.0%
GAAGTAGAAGAAAGCAGTGG+GGG - chr4_4:68940706-68940725 None:intergenic 45.0%
!!! CGGATTTTGACTTGGAGGAA+TGG + chr4_4:68941027-68941046 Msa0698590:intron 45.0%
TCTCAGCTCTTGAACTTGAG+GGG - chr4_4:68941095-68941114 None:intergenic 45.0%
GTCTCAGCTCTTGAACTTGA+GGG - chr4_4:68941096-68941115 None:intergenic 45.0%
TGTCTCAGCTCTTGAACTTG+AGG - chr4_4:68941097-68941116 None:intergenic 45.0%
TCTGTATGAACACGGAAAGG+AGG - chr4_4:68941688-68941707 None:intergenic 45.0%
CGTGTTCATACAGATTGGCA+AGG + chr4_4:68941694-68941713 Msa0698590:intron 45.0%
ATACAGATTGGCAAGGCATG+TGG + chr4_4:68941701-68941720 Msa0698590:intron 45.0%
TCCCAGGTAAGTTTCCACAA+TGG - chr4_4:68942490-68942509 None:intergenic 45.0%
TGGAAACTTACCTGGGACTA+AGG + chr4_4:68942493-68942512 Msa0698590:intron 45.0%
TCTGATGCTTTGCAGGAAAG+CGG + chr4_4:68942888-68942907 Msa0698590:intron 45.0%
!! AATAAAAAAAATTAAAGTAA+AGG + chr4_4:68942435-68942454 Msa0698590:intron 5.0%
!! ATAAAAAAAATTAAAGTAAA+GGG + chr4_4:68942436-68942455 Msa0698590:intron 5.0%
AAAGCAGTGGGGGGTTTCTT+CGG - chr4_4:68940696-68940715 None:intergenic 50.0%
CTCAGCTCTTGAACTTGAGG+GGG - chr4_4:68941094-68941113 None:intergenic 50.0%
TCTCCTCTGGTTGGAATTCC+AGG + chr4_4:68942204-68942223 Msa0698590:intron 50.0%
AACTGATGATCATGCCAGCC+TGG - chr4_4:68942225-68942244 None:intergenic 50.0%
CACGTAAACTGGGTGCTCTA+AGG - chr4_4:68942392-68942411 None:intergenic 50.0%
AGCTTCTTCTCGATAGCAGC+AGG - chr4_4:68942529-68942548 None:intergenic 50.0%
GCAAGAAGGAAACACCGCTT+TGG + chr4_4:68942676-68942695 Msa0698590:intron 50.0%
GACTTGGAGGAATGGTGCAG+AGG + chr4_4:68941035-68941054 Msa0698590:intron 55.0%
TTGGAGGAATGGTGCAGAGG+AGG + chr4_4:68941038-68941057 Msa0698590:intron 55.0%
GCAAGGCATGTGGAGAGAAG+TGG + chr4_4:68941711-68941730 Msa0698590:CDS 55.0%
AGTCGCAGAACTGTCTCCTC+TGG + chr4_4:68942191-68942210 Msa0698590:intron 55.0%
GCAGAACTGTCTCCTCTGGT+TGG + chr4_4:68942195-68942214 Msa0698590:intron 55.0%
CAGCCTGGAATTCCAACCAG+AGG - chr4_4:68942210-68942229 None:intergenic 55.0%
CTCTGGTTGGAATTCCAGGC+TGG + chr4_4:68942208-68942227 Msa0698590:intron 55.0%
CTTTCACGTGCCTTAGTCCC+AGG - chr4_4:68942506-68942525 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 68940606 68942953 68940606 ID=Msa0698590;Name=Msa0698590
chr4_4 mRNA 68940606 68942953 68940606 ID=Msa0698590-mRNA-1;Parent=Msa0698590;Name=Msa0698590-mRNA-1;_AED=0.42;_eAED=0.42;_QI=0|0|0|1|1|1|5|0|118
chr4_4 exon 68940606 68940727 68940606 ID=Msa0698590-mRNA-1:exon:20561;Parent=Msa0698590-mRNA-1
chr4_4 exon 68941371 68941428 68941371 ID=Msa0698590-mRNA-1:exon:20562;Parent=Msa0698590-mRNA-1
chr4_4 exon 68941707 68941751 68941707 ID=Msa0698590-mRNA-1:exon:20563;Parent=Msa0698590-mRNA-1
chr4_4 exon 68942734 68942814 68942734 ID=Msa0698590-mRNA-1:exon:20564;Parent=Msa0698590-mRNA-1
chr4_4 exon 68942903 68942953 68942903 ID=Msa0698590-mRNA-1:exon:20565;Parent=Msa0698590-mRNA-1
chr4_4 CDS 68940606 68940727 68940606 ID=Msa0698590-mRNA-1:cds;Parent=Msa0698590-mRNA-1
chr4_4 CDS 68941371 68941428 68941371 ID=Msa0698590-mRNA-1:cds;Parent=Msa0698590-mRNA-1
chr4_4 CDS 68941707 68941751 68941707 ID=Msa0698590-mRNA-1:cds;Parent=Msa0698590-mRNA-1
chr4_4 CDS 68942734 68942814 68942734 ID=Msa0698590-mRNA-1:cds;Parent=Msa0698590-mRNA-1
chr4_4 CDS 68942903 68942953 68942903 ID=Msa0698590-mRNA-1:cds;Parent=Msa0698590-mRNA-1
Gene Sequence

>Msa0698590

ATGAAGAGATCGTTGCGCCAGACTAAGTCAAGAGACTTAACCAAAGCAGTGCAAAAAAAGAACAAGCAAAAGATTGATGCTAAGAAGCCGAAGAAACCCCCCACTGCTTTCTTCTACTTCTTGGAGGATTTTCGGAAAGAGTTTAAAGATCAGAATCCAGATGTTAAGTCAATGCGTGATATTGGCAAGGCATGTGGAGAGAAGTGGAAAACAATGACTTTTGAGGAGAAAGTTCAATACTATGATATAGCAACCGAGAAACGTGCAGAATTTGATAGAGCAACGACAGAGTATAACAAGAAAATGGAAAGCGGCGAATATGAAGAAACTGATGAGGAGTCAGAGTATGACGAGTAA

Protein sequence

>Msa0698590

MKRSLRQTKSRDLTKAVQKKNKQKIDAKKPKKPPTAFFYFLEDFRKEFKDQNPDVKSMRDIGKACGEKWKTMTFEEKVQYYDIATEKRAEFDRATTEYNKKMESGEYEETDEESEYDE*