Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0699990 | XP_013446497.1 | 100.000 | 69 | 0 | 0 | 1 | 69 | 1 | 69 | 7.43e-41 | 138 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0699990 | sp|O22860|RL38_ARATH | 91.304 | 69 | 6 | 0 | 1 | 69 | 1 | 69 | 1.31e-40 | 130 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0699990 | A0A2Z6P5F7 | 100.000 | 69 | 0 | 0 | 1 | 69 | 1 | 69 | 3.55e-41 | 138 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0699190 | Msa0699990 | 0.807092 | 5.984542e-50 | -8.615850e-47 |
| Msa0699990 | Msa1369190 | 0.804818 | 1.799998e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0699990 | MtrunA17_Chr8g0376751 | 100.000 | 69 | 0 | 0 | 1 | 69 | 1 | 69 | 6.83e-45 | 138 |
| Msa0699990 | MtrunA17_Chr4g0033761 | 94.203 | 69 | 4 | 0 | 1 | 69 | 1 | 69 | 3.74e-42 | 131 |
| Msa0699990 | MtrunA17_Chr2g0285451 | 89.855 | 69 | 7 | 0 | 1 | 69 | 1 | 69 | 6.17e-40 | 125 |
| Msa0699990 | MtrunA17_Chr7g0216191 | 85.507 | 69 | 10 | 0 | 1 | 69 | 1 | 69 | 3.44e-35 | 113 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0699990 | AT2G43460.1 | 91.304 | 69 | 6 | 0 | 1 | 69 | 1 | 69 | 1.33e-41 | 130 |
| Msa0699990 | AT3G59540.1 | 91.304 | 69 | 6 | 0 | 1 | 69 | 1 | 69 | 1.33e-41 | 130 |
Find 23 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATTGATGTTATGGTTAATA+TGG | 0.267232 | 4_4:-70802326 | Msa0699990:three_prime_UTR |
| ATTTAGCTTAATTGATGTTA+TGG | 0.292742 | 4_4:-70802335 | Msa0699990:three_prime_UTR |
| GTTTGTTGTGGCTGCAAATA+GGG | 0.325314 | 4_4:+70802852 | None:intergenic |
| CTATGAGATTTGAAGATGTT+TGG | 0.339052 | 4_4:-70802374 | Msa0699990:three_prime_UTR |
| GAAGCAGATCCACGAAATTA+AGG | 0.348108 | 4_4:-70802708 | Msa0699990:CDS |
| AATTCCAAGTCACAAAATCT+AGG | 0.350296 | 4_4:+70802441 | None:intergenic |
| GGGTTTCTTCCTCGCCTCTT+TGG | 0.396940 | 4_4:+70802872 | None:intergenic |
| GGTTTGTTGTGGCTGCAAAT+AGG | 0.397474 | 4_4:+70802851 | None:intergenic |
| GAAGAAAATCCTTAATTTCG+TGG | 0.409572 | 4_4:+70802699 | None:intergenic |
| TCTTCTGACGGCGCGTAGAA+AGG | 0.443174 | 4_4:-70802681 | Msa0699990:CDS |
| ACCATTGATGTGGTTTGTTG+TGG | 0.495626 | 4_4:+70802840 | None:intergenic |
| AGTGAAAATCAAGAGAAGTA+AGG | 0.506775 | 4_4:-70802648 | Msa0699990:CDS |
| GATATTTATTGCTTATATGA+TGG | 0.507627 | 4_4:-70802266 | Msa0699990:three_prime_UTR |
| TAAGGATGTGGTGAAGTTCA+AGG | 0.511754 | 4_4:-70802630 | Msa0699990:CDS |
| ATTCCAAGTCACAAAATCTA+GGG | 0.522380 | 4_4:+70802442 | None:intergenic |
| TTCTGAAGCTTATTCCAAAG+AGG | 0.534150 | 4_4:-70802886 | None:intergenic |
| GCCACAACAAACCACATCAA+TGG | 0.556817 | 4_4:-70802841 | Msa0699990:intron |
| TTGAAGCAATCTCTTCCACC+TGG | 0.570275 | 4_4:-70802548 | Msa0699990:CDS |
| AATCAAGAGAAGTAAGGATG+TGG | 0.579591 | 4_4:-70802642 | Msa0699990:CDS |
| AGTGGGAAGTACCATTGATG+TGG | 0.614301 | 4_4:+70802830 | None:intergenic |
| AGATCTTGAACAGTCAAACC+AGG | 0.648645 | 4_4:+70802530 | None:intergenic |
| AAGCTTATTCCAAAGAGGCG+AGG | 0.669151 | 4_4:-70802881 | Msa0699990:five_prime_UTR |
| TCTTGAACAGTCAAACCAGG+TGG | 0.706952 | 4_4:+70802533 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAGTATTTTTTGTGTTAATA+TGG | - | chr4_4:70802780-70802799 | Msa0699990:intron | 15.0% |
| !!! | TTGGAATTATGTTTTGTAAT+TGG | - | chr4_4:70802657-70802676 | Msa0699990:CDS | 20.0% |
| !!! | ATTTAGCTTAATTGATGTTA+TGG | - | chr4_4:70802748-70802767 | Msa0699990:intron | 20.0% |
| !! | AATTGATGTTATGGTTAATA+TGG | - | chr4_4:70802757-70802776 | Msa0699990:intron | 20.0% |
| !! | GATATTTATTGCTTATATGA+TGG | - | chr4_4:70802817-70802836 | Msa0699990:intron | 20.0% |
| !!! | ATGTATTGTGTTGTTTTTTC+AGG | - | chr4_4:70802351-70802370 | Msa0699990:three_prime_UTR | 25.0% |
| !! | AATTAAGGATTTTCTTCTGA+CGG | - | chr4_4:70802390-70802409 | Msa0699990:three_prime_UTR | 25.0% |
| TCCAATTAACGAAAGAAAGT+GGG | + | chr4_4:70802273-70802292 | None:intergenic | 30.0% | |
| ! | TGAATCTACAACGATGATTT+TGG | - | chr4_4:70802314-70802333 | Msa0699990:three_prime_UTR | 30.0% |
| GAAGAAAATCCTTAATTTCG+TGG | + | chr4_4:70802387-70802406 | None:intergenic | 30.0% | |
| AGTGAAAATCAAGAGAAGTA+AGG | - | chr4_4:70802435-70802454 | Msa0699990:three_prime_UTR | 30.0% | |
| ATTCCAAGTCACAAAATCTA+GGG | + | chr4_4:70802644-70802663 | None:intergenic | 30.0% | |
| AATTCCAAGTCACAAAATCT+AGG | + | chr4_4:70802645-70802664 | None:intergenic | 30.0% | |
| CTATGAGATTTGAAGATGTT+TGG | - | chr4_4:70802709-70802728 | Msa0699990:CDS | 30.0% | |
| TCCCACTTTCTTTCGTTAAT+TGG | - | chr4_4:70802269-70802288 | Msa0699990:three_prime_UTR | 35.0% | |
| CTCCAATTAACGAAAGAAAG+TGG | + | chr4_4:70802274-70802293 | None:intergenic | 35.0% | |
| AATCAAGAGAAGTAAGGATG+TGG | - | chr4_4:70802441-70802460 | Msa0699990:three_prime_UTR | 35.0% | |
| ! | AAACCCTAGATTTTGTGACT+TGG | - | chr4_4:70802638-70802657 | Msa0699990:CDS | 35.0% |
| !! | GTTTGTTGTGGCTGCAAATA+GGG | + | chr4_4:70802234-70802253 | None:intergenic | 40.0% |
| !! | ACCATTGATGTGGTTTGTTG+TGG | + | chr4_4:70802246-70802265 | None:intergenic | 40.0% |
| GAAGCAGATCCACGAAATTA+AGG | - | chr4_4:70802375-70802394 | Msa0699990:three_prime_UTR | 40.0% | |
| TAAGGATGTGGTGAAGTTCA+AGG | - | chr4_4:70802453-70802472 | Msa0699990:three_prime_UTR | 40.0% | |
| AGATCTTGAACAGTCAAACC+AGG | + | chr4_4:70802556-70802575 | None:intergenic | 40.0% | |
| AAGCTTATTCCAAAGAGGCG+AGG | - | chr4_4:70802202-70802221 | Msa0699990:three_prime_UTR | 45.0% | |
| !! | GGTTTGTTGTGGCTGCAAAT+AGG | + | chr4_4:70802235-70802254 | None:intergenic | 45.0% |
| GCCACAACAAACCACATCAA+TGG | - | chr4_4:70802242-70802261 | Msa0699990:three_prime_UTR | 45.0% | |
| !! | AGTGGGAAGTACCATTGATG+TGG | + | chr4_4:70802256-70802275 | None:intergenic | 45.0% |
| TTGAAGCAATCTCTTCCACC+TGG | - | chr4_4:70802535-70802554 | Msa0699990:CDS | 45.0% | |
| TCTTGAACAGTCAAACCAGG+TGG | + | chr4_4:70802553-70802572 | None:intergenic | 45.0% | |
| GGGTTTCTTCCTCGCCTCTT+TGG | + | chr4_4:70802214-70802233 | None:intergenic | 55.0% | |
| TCTTCTGACGGCGCGTAGAA+AGG | - | chr4_4:70802402-70802421 | Msa0699990:three_prime_UTR | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_4 | gene | 70802201 | 70802904 | 70802201 | ID=Msa0699990;Name=Msa0699990 |
| chr4_4 | mRNA | 70802201 | 70802904 | 70802201 | ID=Msa0699990-mRNA-1;Parent=Msa0699990;Name=Msa0699990-mRNA-1;_AED=0.00;_eAED=0.00;_QI=60|1|1|1|1|1|2|325|69 |
| chr4_4 | exon | 70802201 | 70802732 | 70802201 | ID=Msa0699990-mRNA-1:exon:21485;Parent=Msa0699990-mRNA-1 |
| chr4_4 | exon | 70802842 | 70802904 | 70802842 | ID=Msa0699990-mRNA-1:exon:21484;Parent=Msa0699990-mRNA-1 |
| chr4_4 | five_prime_UTR | 70802845 | 70802904 | 70802845 | ID=Msa0699990-mRNA-1:five_prime_utr;Parent=Msa0699990-mRNA-1 |
| chr4_4 | CDS | 70802842 | 70802844 | 70802842 | ID=Msa0699990-mRNA-1:cds;Parent=Msa0699990-mRNA-1 |
| chr4_4 | CDS | 70802526 | 70802732 | 70802526 | ID=Msa0699990-mRNA-1:cds;Parent=Msa0699990-mRNA-1 |
| chr4_4 | three_prime_UTR | 70802201 | 70802525 | 70802201 | ID=Msa0699990-mRNA-1:three_prime_utr;Parent=Msa0699990-mRNA-1 |
| Gene Sequence |
| Protein sequence |
