Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0707920 | AET04117.1 | 94.444 | 90 | 2 | 3 | 3 | 89 | 13 | 102 | 4.58e-48 | 158 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0707920 | G7LH58 | 94.444 | 90 | 2 | 3 | 3 | 89 | 13 | 102 | 2.19e-48 | 158 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0707920 | Msa1131430 | 0.801545 | 8.558650e-49 | -8.615850e-47 |
| Msa0273400 | Msa0707920 | 0.812447 | 4.221060e-51 | -8.615850e-47 |
| Msa0313660 | Msa0707920 | 0.813724 | 2.214110e-51 | -8.615850e-47 |
| Msa0560100 | Msa0707920 | 0.814639 | 1.390906e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0707920 | MtrunA17_Chr8g0390581 | 94.444 | 90 | 2 | 3 | 3 | 89 | 13 | 102 | 4.22e-52 | 158 |
| Msa0707920 | MtrunA17_Chr6g0485391 | 55.435 | 92 | 34 | 4 | 1 | 89 | 1 | 88 | 2.61e-22 | 82.4 |
| Msa0707920 | MtrunA17_Chr5g0412611 | 50.000 | 58 | 17 | 2 | 33 | 87 | 26 | 74 | 4.86e-11 | 53.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 24 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGACACATGAAGGGTCTTTC+AGG | 0.198905 | 4_4:+81373360 | Msa0707920:CDS |
| GCCGATTCCACTGTCACTTT+TGG | 0.208493 | 4_4:-81373291 | None:intergenic |
| GAACATAGACACCGTCCTTT+GGG | 0.403670 | 4_4:-81373332 | None:intergenic |
| ATGATTAGGAGGCATGGATT+TGG | 0.406829 | 4_4:-81373444 | None:intergenic |
| TGAACATAGACACCGTCCTT+TGG | 0.409239 | 4_4:-81373333 | None:intergenic |
| TTAATTTGAGGTTGAGATTG+AGG | 0.413522 | 4_4:-81373245 | None:intergenic |
| GAAACAACAGACTTATGATT+AGG | 0.415127 | 4_4:-81373458 | None:intergenic |
| CACCTCCACCTTGGATGAAA+AGG | 0.419918 | 4_4:+81373420 | Msa0707920:CDS |
| CCCAAAAGTGACAGTGGAAT+CGG | 0.461245 | 4_4:+81373290 | Msa0707920:CDS |
| GTTGAGATTGAGGTTGAGAT+TGG | 0.469836 | 4_4:-81373235 | None:intergenic |
| CGTCATCTGCACCTCCACCT+TGG | 0.488683 | 4_4:+81373411 | Msa0707920:CDS |
| TTGAGATTGAGGTTGAGATT+GGG | 0.491587 | 4_4:-81373234 | None:intergenic |
| TGTTCACCAACGACACATGA+AGG | 0.528018 | 4_4:+81373350 | Msa0707920:CDS |
| CAGTGGAATCGGCGATGCGG+CGG | 0.550074 | 4_4:+81373301 | Msa0707920:CDS |
| AGACTTATGATTAGGAGGCA+TGG | 0.556169 | 4_4:-81373450 | None:intergenic |
| GAAACGATGAGCTTAATTTG+AGG | 0.569661 | 4_4:-81373257 | None:intergenic |
| ACAACAGACTTATGATTAGG+AGG | 0.591566 | 4_4:-81373455 | None:intergenic |
| CGGCGTCGTTTCCCAAAGGA+CGG | 0.604873 | 4_4:+81373321 | Msa0707920:CDS |
| GCGGCGGCGTCGTTTCCCAA+AGG | 0.615082 | 4_4:+81373317 | Msa0707920:CDS |
| AAAGACCCTTCATGTGTCGT+TGG | 0.628957 | 4_4:-81373356 | None:intergenic |
| GTTCACCAACGACACATGAA+GGG | 0.648482 | 4_4:+81373351 | Msa0707920:CDS |
| TCATCACCCAAAAGTGACAG+TGG | 0.653040 | 4_4:+81373284 | Msa0707920:CDS |
| TGACAGTGGAATCGGCGATG+CGG | 0.659679 | 4_4:+81373298 | Msa0707920:CDS |
| AAACATATAGTAACTCACTG+TGG | 0.708803 | 4_4:-81373488 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTAATTTGAGGTTGAGATTG+AGG | - | chr4_4:81373248-81373267 | None:intergenic | 30.0% |
| GAAACAACAGACTTATGATT+AGG | - | chr4_4:81373461-81373480 | None:intergenic | 30.0% | |
| TTGAGATTGAGGTTGAGATT+GGG | - | chr4_4:81373237-81373256 | None:intergenic | 35.0% | |
| ! | GAAACGATGAGCTTAATTTG+AGG | - | chr4_4:81373260-81373279 | None:intergenic | 35.0% |
| ACAACAGACTTATGATTAGG+AGG | - | chr4_4:81373458-81373477 | None:intergenic | 35.0% | |
| GTTGAGATTGAGGTTGAGAT+TGG | - | chr4_4:81373238-81373257 | None:intergenic | 40.0% | |
| !! | GATTTGGTCCTTTTCATCCA+AGG | - | chr4_4:81373431-81373450 | None:intergenic | 40.0% |
| ! | ATGATTAGGAGGCATGGATT+TGG | - | chr4_4:81373447-81373466 | None:intergenic | 40.0% |
| AGACTTATGATTAGGAGGCA+TGG | - | chr4_4:81373453-81373472 | None:intergenic | 40.0% | |
| TCATCACCCAAAAGTGACAG+TGG | + | chr4_4:81373284-81373303 | Msa0707920:CDS | 45.0% | |
| !!! | CCGATTCCACTGTCACTTTT+GGG | - | chr4_4:81373293-81373312 | None:intergenic | 45.0% |
| CCCAAAAGTGACAGTGGAAT+CGG | + | chr4_4:81373290-81373309 | Msa0707920:CDS | 45.0% | |
| GAACATAGACACCGTCCTTT+GGG | - | chr4_4:81373335-81373354 | None:intergenic | 45.0% | |
| TGAACATAGACACCGTCCTT+TGG | - | chr4_4:81373336-81373355 | None:intergenic | 45.0% | |
| TGTTCACCAACGACACATGA+AGG | + | chr4_4:81373350-81373369 | Msa0707920:CDS | 45.0% | |
| GTTCACCAACGACACATGAA+GGG | + | chr4_4:81373351-81373370 | Msa0707920:CDS | 45.0% | |
| AAAGACCCTTCATGTGTCGT+TGG | - | chr4_4:81373359-81373378 | None:intergenic | 45.0% | |
| !!! | AGGTGTCGTTTTCATCGATC+AGG | + | chr4_4:81373380-81373399 | Msa0707920:CDS | 45.0% |
| !!! | GGTGTCGTTTTCATCGATCA+GGG | + | chr4_4:81373381-81373400 | Msa0707920:CDS | 45.0% |
| ! | TTGGTCCTTTTCATCCAAGG+TGG | - | chr4_4:81373428-81373447 | None:intergenic | 45.0% |
| !!! | GCCGATTCCACTGTCACTTT+TGG | - | chr4_4:81373294-81373313 | None:intergenic | 50.0% |
| CGACACATGAAGGGTCTTTC+AGG | + | chr4_4:81373360-81373379 | Msa0707920:CDS | 50.0% | |
| CACCTCCACCTTGGATGAAA+AGG | + | chr4_4:81373420-81373439 | Msa0707920:CDS | 50.0% | |
| ! | GTCCTTTTCATCCAAGGTGG+AGG | - | chr4_4:81373425-81373444 | None:intergenic | 50.0% |
| TGACAGTGGAATCGGCGATG+CGG | + | chr4_4:81373298-81373317 | Msa0707920:CDS | 55.0% | |
| CGGCGTCGTTTCCCAAAGGA+CGG | + | chr4_4:81373321-81373340 | Msa0707920:CDS | 60.0% | |
| CGTCATCTGCACCTCCACCT+TGG | + | chr4_4:81373411-81373430 | Msa0707920:CDS | 60.0% | |
| CAGTGGAATCGGCGATGCGG+CGG | + | chr4_4:81373301-81373320 | Msa0707920:CDS | 65.0% | |
| GCGGCGGCGTCGTTTCCCAA+AGG | + | chr4_4:81373317-81373336 | Msa0707920:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_4 | gene | 81373227 | 81373496 | 81373227 | ID=Msa0707920;Name=Msa0707920 |
| chr4_4 | mRNA | 81373227 | 81373496 | 81373227 | ID=Msa0707920-mRNA-1;Parent=Msa0707920;Name=Msa0707920-mRNA-1;_AED=0.38;_eAED=0.40;_QI=0|-1|0|1|-1|1|1|0|89 |
| chr4_4 | exon | 81373227 | 81373496 | 81373227 | ID=Msa0707920-mRNA-1:exon:26606;Parent=Msa0707920-mRNA-1 |
| chr4_4 | CDS | 81373227 | 81373496 | 81373227 | ID=Msa0707920-mRNA-1:cds;Parent=Msa0707920-mRNA-1 |
| Gene Sequence |
| Protein sequence |
