Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0313660 | XP_003593732.1 | 98.750 | 80 | 1 | 0 | 1 | 80 | 1 | 80 | 6.80e-46 | 151 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0313660 | sp|O82275|TAX1_ARATH | 75.000 | 76 | 17 | 1 | 5 | 80 | 2 | 75 | 3.41e-26 | 94.4 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0313660 | G7IKA1 | 98.750 | 80 | 1 | 0 | 1 | 80 | 1 | 80 | 3.25e-46 | 151 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0025560 | Msa0313660 | 0.801901 | 7.234640e-49 | -8.615850e-47 |
| Msa0042520 | Msa0313660 | -0.807700 | 4.446067e-50 | -8.615850e-47 |
| Msa0085450 | Msa0313660 | 0.808876 | 2.496667e-50 | -8.615850e-47 |
| Msa0192870 | Msa0313660 | 0.843565 | 1.296248e-58 | -8.615850e-47 |
| Msa0235000 | Msa0313660 | 0.895894 | 6.210551e-76 | -8.615850e-47 |
| Msa0267700 | Msa0313660 | 0.807172 | 5.755322e-50 | -8.615850e-47 |
| Msa0273400 | Msa0313660 | 0.970713 | 4.899112e-132 | -8.615850e-47 |
| Msa0313660 | Msa0331580 | 0.802128 | 6.498020e-49 | -8.615850e-47 |
| Msa0313660 | Msa0405200 | 0.823349 | 1.453282e-53 | -8.615850e-47 |
| Msa0313660 | Msa0433050 | 0.827618 | 1.416405e-54 | -8.615850e-47 |
| Msa0313660 | Msa0449500 | 0.823145 | 1.621507e-53 | -8.615850e-47 |
| Msa0313660 | Msa0464150 | 0.801517 | 8.676444e-49 | -8.615850e-47 |
| Msa0313660 | Msa0497680 | 0.816958 | 4.227485e-52 | -8.615850e-47 |
| Msa0313660 | Msa0509640 | 0.806600 | 7.603570e-50 | -8.615850e-47 |
| Msa0313660 | Msa0524670 | 0.831745 | 1.402807e-55 | -8.615850e-47 |
| Msa0313660 | Msa0529110 | 0.829881 | 4.017165e-55 | -8.615850e-47 |
| Msa0313660 | Msa0547370 | 0.807875 | 4.081535e-50 | -8.615850e-47 |
| Msa0313660 | Msa0568920 | 0.812930 | 3.307887e-51 | -8.615850e-47 |
| Msa0313660 | Msa0581350 | 0.808740 | 2.669170e-50 | -8.615850e-47 |
| Msa0313660 | Msa0590620 | -0.800229 | 1.589541e-48 | -8.615850e-47 |
| Msa0313660 | Msa0595270 | 0.827857 | 1.240793e-54 | -8.615850e-47 |
| Msa0313660 | Msa0597800 | 0.809935 | 1.479523e-50 | -8.615850e-47 |
| Msa0313660 | Msa0644340 | 0.807511 | 4.878136e-50 | -8.615850e-47 |
| Msa0313660 | Msa0682910 | 0.811119 | 8.210649e-51 | -8.615850e-47 |
| Msa0313660 | Msa0707920 | 0.813724 | 2.214110e-51 | -8.615850e-47 |
| Msa0313660 | Msa0714230 | -0.836471 | 9.178491e-57 | -8.615850e-47 |
| Msa0313660 | Msa0743370 | 0.819359 | 1.210421e-52 | -8.615850e-47 |
| Msa0313660 | Msa0773030 | 0.820021 | 8.546025e-53 | -8.615850e-47 |
| Msa0313660 | Msa0777600 | 0.805939 | 1.047859e-49 | -8.615850e-47 |
| Msa0313660 | Msa0795320 | 0.807643 | 4.573393e-50 | -8.615850e-47 |
| Msa0313660 | Msa0814660 | 0.810807 | 9.592343e-51 | -8.615850e-47 |
| Msa0313660 | Msa0817880 | -0.802727 | 4.890376e-49 | -8.615850e-47 |
| Msa0313660 | Msa0840540 | 0.804946 | 1.692231e-49 | -8.615850e-47 |
| Msa0313660 | Msa0850640 | 0.813853 | 2.074287e-51 | -8.615850e-47 |
| Msa0313660 | Msa0855260 | 0.826157 | 3.163767e-54 | -8.615850e-47 |
| Msa0313660 | Msa0873910 | 0.824839 | 6.491988e-54 | -8.615850e-47 |
| Msa0313660 | Msa0906660 | 0.821951 | 3.073045e-53 | -8.615850e-47 |
| Msa0313660 | Msa0925940 | 0.823125 | 1.639244e-53 | -8.615850e-47 |
| Msa0313660 | Msa0985980 | -0.809028 | 2.316770e-50 | -8.615850e-47 |
| Msa0313660 | Msa1034690 | 0.854536 | 1.153316e-61 | -8.615850e-47 |
| Msa0313660 | Msa1044520 | -0.813456 | 2.536935e-51 | -8.615850e-47 |
| Msa0313660 | Msa1062060 | 0.818522 | 1.876163e-52 | -8.615850e-47 |
| Msa0313660 | Msa1074550 | 0.810507 | 1.113931e-50 | -8.615850e-47 |
| Msa0313660 | Msa1080130 | 0.806879 | 6.638139e-50 | -8.615850e-47 |
| Msa0313660 | Msa1089990 | 0.824791 | 6.666811e-54 | -8.615850e-47 |
| Msa0313660 | Msa1107220 | 0.848943 | 4.440262e-60 | -8.615850e-47 |
| Msa0313660 | Msa1107250 | 0.819847 | 9.367742e-53 | -8.615850e-47 |
| Msa0313660 | Msa1136690 | 0.804983 | 1.662289e-49 | -8.615850e-47 |
| Msa0313660 | Msa1150740 | 0.801166 | 1.023631e-48 | -8.615850e-47 |
| Msa0313660 | Msa1150760 | 0.835607 | 1.520286e-56 | -8.615850e-47 |
| Msa0313660 | Msa1162050 | 0.804009 | 2.652972e-49 | -8.615850e-47 |
| Msa0313660 | Msa1197230 | 0.806795 | 6.914827e-50 | -8.615850e-47 |
| Msa0313660 | Msa1199540 | -0.804730 | 1.877492e-49 | -8.615850e-47 |
| Msa0313660 | Msa1202110 | 0.819510 | 1.118202e-52 | -8.615850e-47 |
| Msa0313660 | Msa1221270 | 0.818992 | 1.467703e-52 | -8.615850e-47 |
| Msa0313660 | Msa1223710 | 0.829306 | 5.541473e-55 | -8.615850e-47 |
| Msa0313660 | Msa1254870 | 0.820354 | 7.170408e-53 | -8.615850e-47 |
| Msa0313660 | Msa1263370 | 0.809751 | 1.620766e-50 | -8.615850e-47 |
| Msa0313660 | Msa1264300 | -0.821287 | 4.374102e-53 | -8.615850e-47 |
| Msa0313660 | Msa1279200 | -0.803930 | 2.755640e-49 | -8.615850e-47 |
| Msa0313660 | Msa1302630 | 0.805271 | 1.446639e-49 | -8.615850e-47 |
| Msa0313660 | Msa1328430 | 0.821737 | 3.443982e-53 | -8.615850e-47 |
| Msa0313660 | Msa1328680 | -0.812466 | 4.180513e-51 | -8.615850e-47 |
| Msa0313660 | Msa1334350 | -0.825700 | 4.062183e-54 | -8.615850e-47 |
| Msa0313660 | Msa1362520 | 0.817466 | 3.250862e-52 | -8.615850e-47 |
| Msa0313660 | Msa1402340 | -0.827050 | 1.937690e-54 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0313660 | MtrunA17_Chr2g0283451 | 98.750 | 80 | 1 | 0 | 1 | 80 | 1 | 80 | 6.26e-50 | 151 |
| Msa0313660 | MtrunA17_Chr2g0325441 | 73.684 | 76 | 20 | 0 | 5 | 80 | 2 | 77 | 4.07e-35 | 114 |
| Msa0313660 | MtrunA17_Chr4g0040991 | 75.000 | 76 | 19 | 0 | 5 | 80 | 2 | 77 | 5.92e-35 | 113 |
| Msa0313660 | MtrunA17_Chr2g0324911 | 59.459 | 74 | 30 | 0 | 7 | 80 | 5 | 78 | 9.40e-21 | 77.8 |
| Msa0313660 | MtrunA17_Chr4g0040491 | 56.944 | 72 | 31 | 0 | 9 | 80 | 8 | 79 | 1.61e-14 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0313660 | AT2G31090.1 | 75.000 | 76 | 17 | 1 | 5 | 80 | 2 | 75 | 3.46e-27 | 94.4 |
| Msa0313660 | AT2G20562.1 | 60.870 | 69 | 27 | 0 | 12 | 80 | 5 | 73 | 5.39e-20 | 76.3 |
Find 28 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTTTGTAGGTGCAGTTATT+TGG | 0.227999 | 2_4:+9087854 | Msa0313660:CDS |
| AACCACTCCATAACCTTAAT+TGG | 0.239487 | 2_4:-9088078 | None:intergenic |
| TCTCTCATAATCTCCTTTGT+AGG | 0.287883 | 2_4:+9087841 | Msa0313660:CDS |
| TCTCATCAAGGCTCCAATTA+AGG | 0.314893 | 2_4:+9088065 | Msa0313660:CDS |
| CACATGGTTAATTACCTAAT+GGG | 0.332162 | 2_4:-9087748 | None:intergenic |
| ACACATGGTTAATTACCTAA+TGG | 0.347508 | 2_4:-9087749 | None:intergenic |
| TTTAAGAACTTCAACCCATT+AGG | 0.359993 | 2_4:+9087734 | None:intergenic |
| CAAGGCTCCAATTAAGGTTA+TGG | 0.360751 | 2_4:+9088071 | Msa0313660:CDS |
| TCTATTCTAACATGGAATCT+TGG | 0.376692 | 2_4:-9088106 | None:intergenic |
| GGTGCAGTTATTTGGATTGT+TGG | 0.388294 | 2_4:+9087862 | Msa0313660:CDS |
| GTGCAGTTATTTGGATTGTT+GGG | 0.397665 | 2_4:+9087863 | Msa0313660:CDS |
| GTTTGCTCTGGCTCTCATCA+AGG | 0.420977 | 2_4:+9088053 | Msa0313660:CDS |
| CCTCATAGTTGAGTTTGCTC+TGG | 0.438328 | 2_4:+9088041 | Msa0313660:CDS |
| GATTGTAAATTCAGGCCTCT+TGG | 0.461509 | 2_4:+9087787 | Msa0313660:CDS |
| ATGAGAGACAAGAAAGCAAA+AGG | 0.478563 | 2_4:-9087826 | None:intergenic |
| AGAAAGCAAAAGGAAGGCCT+AGG | 0.499755 | 2_4:-9087816 | None:intergenic |
| TTAACCATGTGTTGCTGTTG+TGG | 0.511293 | 2_4:+9087760 | Msa0313660:exon |
| CCAGAGCAAACTCAACTATG+AGG | 0.515083 | 2_4:-9088041 | None:intergenic |
| GTGGTGAGGATTGTAAATTC+AGG | 0.531054 | 2_4:+9087779 | Msa0313660:CDS |
| CCAAATAACTGCACCTACAA+AGG | 0.535186 | 2_4:-9087854 | None:intergenic |
| ATATCTCTTCTATTCTAACA+TGG | 0.596081 | 2_4:-9088114 | None:intergenic |
| CATGTGTTGCTGTTGTGGTG+AGG | 0.609312 | 2_4:+9087765 | Msa0313660:exon |
| GAGACAAGAAAGCAAAAGGA+AGG | 0.614663 | 2_4:-9087822 | None:intergenic |
| AGGGTCACGCAAAGACAGCA+TGG | 0.623057 | 2_4:-9088021 | None:intergenic |
| CAGAGCAAACTCAACTATGA+GGG | 0.623831 | 2_4:-9088040 | None:intergenic |
| CTCACCACAACAGCAACACA+TGG | 0.630485 | 2_4:-9087764 | None:intergenic |
| CTCCAATTAAGGTTATGGAG+TGG | 0.661349 | 2_4:+9088076 | Msa0313660:CDS |
| AGGCCTAGGAGAAAACCAAG+AGG | 0.747286 | 2_4:-9087802 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAGAAAATACTAATAGAT+GGG | - | chr2_4:9087905-9087924 | None:intergenic | 15.0% |
| !! | CAAAAGAAAATACTAATAGA+TGG | - | chr2_4:9087906-9087925 | None:intergenic | 20.0% |
| ! | AATGTGTATGATGCTATATA+TGG | + | chr2_4:9087973-9087992 | Msa0313660:intron | 25.0% |
| CACATGGTTAATTACCTAAT+GGG | - | chr2_4:9087751-9087770 | None:intergenic | 30.0% | |
| ACACATGGTTAATTACCTAA+TGG | - | chr2_4:9087752-9087771 | None:intergenic | 30.0% | |
| ATGAGAGACAAGAAAGCAAA+AGG | - | chr2_4:9087829-9087848 | None:intergenic | 35.0% | |
| TCTCTCATAATCTCCTTTGT+AGG | + | chr2_4:9087841-9087860 | Msa0313660:CDS | 35.0% | |
| GTGCAGTTATTTGGATTGTT+GGG | + | chr2_4:9087863-9087882 | Msa0313660:CDS | 35.0% | |
| CTAATAGATGGGAAAAGAGA+GGG | - | chr2_4:9087894-9087913 | None:intergenic | 35.0% | |
| ACTAATAGATGGGAAAAGAG+AGG | - | chr2_4:9087895-9087914 | None:intergenic | 35.0% | |
| TGTATGATGCTATATATGGC+AGG | + | chr2_4:9087977-9087996 | Msa0313660:intron | 35.0% | |
| AACCACTCCATAACCTTAAT+TGG | - | chr2_4:9088081-9088100 | None:intergenic | 35.0% | |
| ! | TTAACCATGTGTTGCTGTTG+TGG | + | chr2_4:9087760-9087779 | Msa0313660:exon | 40.0% |
| GTGGTGAGGATTGTAAATTC+AGG | + | chr2_4:9087779-9087798 | Msa0313660:CDS | 40.0% | |
| GATTGTAAATTCAGGCCTCT+TGG | + | chr2_4:9087787-9087806 | Msa0313660:CDS | 40.0% | |
| GAGACAAGAAAGCAAAAGGA+AGG | - | chr2_4:9087825-9087844 | None:intergenic | 40.0% | |
| CCAAATAACTGCACCTACAA+AGG | - | chr2_4:9087857-9087876 | None:intergenic | 40.0% | |
| CCTTTGTAGGTGCAGTTATT+TGG | + | chr2_4:9087854-9087873 | Msa0313660:CDS | 40.0% | |
| GGTGCAGTTATTTGGATTGT+TGG | + | chr2_4:9087862-9087881 | Msa0313660:CDS | 40.0% | |
| CAGAGCAAACTCAACTATGA+GGG | - | chr2_4:9088043-9088062 | None:intergenic | 40.0% | |
| TCTCATCAAGGCTCCAATTA+AGG | + | chr2_4:9088065-9088084 | Msa0313660:CDS | 40.0% | |
| CAAGGCTCCAATTAAGGTTA+TGG | + | chr2_4:9088071-9088090 | Msa0313660:CDS | 40.0% | |
| CTCCAATTAAGGTTATGGAG+TGG | + | chr2_4:9088076-9088095 | Msa0313660:CDS | 40.0% | |
| AGAAAGCAAAAGGAAGGCCT+AGG | - | chr2_4:9087819-9087838 | None:intergenic | 45.0% | |
| CCAGAGCAAACTCAACTATG+AGG | - | chr2_4:9088044-9088063 | None:intergenic | 45.0% | |
| ! | CCTCATAGTTGAGTTTGCTC+TGG | + | chr2_4:9088041-9088060 | Msa0313660:CDS | 45.0% |
| CTCACCACAACAGCAACACA+TGG | - | chr2_4:9087767-9087786 | None:intergenic | 50.0% | |
| ! | CATGTGTTGCTGTTGTGGTG+AGG | + | chr2_4:9087765-9087784 | Msa0313660:exon | 50.0% |
| !! | AGGCCTCTTGGTTTTCTCCT+AGG | + | chr2_4:9087799-9087818 | Msa0313660:CDS | 50.0% |
| AGGCCTAGGAGAAAACCAAG+AGG | - | chr2_4:9087805-9087824 | None:intergenic | 50.0% | |
| !! | GTTTGCTCTGGCTCTCATCA+AGG | + | chr2_4:9088053-9088072 | Msa0313660:CDS | 50.0% |
| AGGGTCACGCAAAGACAGCA+TGG | - | chr2_4:9088024-9088043 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_4 | gene | 9087737 | 9088122 | 9087737 | ID=Msa0313660;Name=Msa0313660 |
| chr2_4 | mRNA | 9087737 | 9088122 | 9087737 | ID=Msa0313660-mRNA-1;Parent=Msa0313660;Name=Msa0313660-mRNA-1;_AED=0.15;_eAED=0.15;_QI=29|1|0.5|1|1|1|2|0|80 |
| chr2_4 | exon | 9087737 | 9087884 | 9087737 | ID=Msa0313660-mRNA-1:exon:3464;Parent=Msa0313660-mRNA-1 |
| chr2_4 | exon | 9087999 | 9088122 | 9087999 | ID=Msa0313660-mRNA-1:exon:3465;Parent=Msa0313660-mRNA-1 |
| chr2_4 | five_prime_UTR | 9087737 | 9087765 | 9087737 | ID=Msa0313660-mRNA-1:five_prime_utr;Parent=Msa0313660-mRNA-1 |
| chr2_4 | CDS | 9087766 | 9087884 | 9087766 | ID=Msa0313660-mRNA-1:cds;Parent=Msa0313660-mRNA-1 |
| chr2_4 | CDS | 9087999 | 9088122 | 9087999 | ID=Msa0313660-mRNA-1:cds;Parent=Msa0313660-mRNA-1 |
| Gene Sequence |
| Protein sequence |
