AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1131430


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1131430 MtrunA17_Chr4g0073821 100.000 77 0 0 1 77 1 77 7.81e-51 153
Msa1131430 MtrunA17_Chr4g0073811 81.333 75 14 0 1 75 1 75 1.22e-40 127
Msa1131430 MtrunA17_Chr7g0271271 70.833 48 13 1 8 55 6 52 2.43e-16 65.9
Msa1131430 MtrunA17_Chr7g0271281 62.500 48 17 1 8 55 6 52 2.29e-14 60.8
Msa1131430 MtrunA17_Chr6g0462381 55.769 52 22 1 5 56 3 53 1.91e-13 58.5
Msa1131430 MtrunA17_Chr7g0271261 61.702 47 17 1 9 55 7 52 1.58e-12 56.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1131430 AT4G28088.1 80.263 76 14 1 1 75 1 76 8.21e-41 128
Msa1131430 AT2G24040.1 79.167 72 15 0 1 72 1 72 6.85e-38 121
Msa1131430 AT4G30660.1 75.342 73 18 0 1 73 1 73 4.70e-37 119
Msa1131430 AT4G30660.2 75.342 73 18 0 1 73 1 73 4.70e-37 119
Msa1131430 AT4G30650.1 63.514 74 26 1 1 74 1 73 2.63e-27 94.4
Msa1131430 AT2G38905.1 66.667 51 16 1 6 56 4 53 1.36e-15 64.3
Msa1131430 AT3G05880.1 69.565 46 13 1 10 55 8 52 1.71e-14 61.6
Msa1131430 AT3G05890.1 67.391 46 14 1 10 55 8 52 2.92e-14 60.8
Msa1131430 AT2G24040.2 75.000 32 8 0 1 32 1 32 4.71e-13 57.8

Find 30 sgRNAs with CRISPR-Local

Find 89 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCTGTGTTGCTCCCTCCTT+TGG 0.237778 7_3:-83971698 Msa1131430:CDS
AGATTTGAGTTAGGTCAATT+TGG 0.259030 7_3:-83971196 Msa1131430:three_prime_UTR
GATCATCACTTGTTCTTGTT+TGG 0.268370 7_3:-83971795 Msa1131430:five_prime_UTR
AATTCATGTAGAAAATTATA+TGG 0.289444 7_3:-83971102 Msa1131430:three_prime_UTR
TGATGTGATTGTTGATTGTT+TGG 0.361552 7_3:-83971079 Msa1131430:three_prime_UTR
ATTGTGGTTTACTCGAAAAC+TGG 0.367118 7_3:-83971150 Msa1131430:three_prime_UTR
ATCACGATCAATAAAGATAA+TGG 0.374759 7_3:+83971353 None:intergenic
GTGATGATCATCTAAAGTTT+AGG 0.388258 7_3:+83971809 None:intergenic
TGCTTGCTCCTTACAATACT+AGG 0.414159 7_3:-83971408 Msa1131430:CDS
CTTGTTCTTGTTTGGATCGC+GGG 0.438467 7_3:-83971787 Msa1131430:five_prime_UTR
TTCTCTTTGAGATTTGAGTT+AGG 0.454642 7_3:-83971205 Msa1131430:three_prime_UTR
TAAAGTGGTTAGTTATATTG+TGG 0.465212 7_3:-83971014 Msa1131430:three_prime_UTR
ACAATACTAGGATATCTTCC+TGG 0.469622 7_3:-83971396 Msa1131430:CDS
GCTGTGTTGCTCCCTCCTTT+GGG 0.470326 7_3:-83971697 Msa1131430:CDS
TTGAGTTAGGTCAATTTGGA+TGG 0.478573 7_3:-83971192 Msa1131430:three_prime_UTR
ATCAGTACTTTGATGAATAT+AGG 0.494017 7_3:-83971332 Msa1131430:CDS
ATCAAATTATGAAAATAAAG+TGG 0.499775 7_3:-83971029 Msa1131430:three_prime_UTR
GAAGATATCCTAGTATTGTA+AGG 0.501846 7_3:+83971400 None:intergenic
TTGCATTATTCGTTTCATTG+TGG 0.556335 7_3:-83971166 Msa1131430:three_prime_UTR
TTGGGTGTTTGTCTCCGCCA+TGG 0.565764 7_3:-83971679 Msa1131430:CDS
ACTTGTTCTTGTTTGGATCG+CGG 0.583202 7_3:-83971788 Msa1131430:five_prime_UTR
CAAGAATAATAAAATTCAAG+TGG 0.594545 7_3:+83971238 None:intergenic
GGAGACAAACACCCAAAGGA+GGG 0.610468 7_3:+83971686 None:intergenic
CGGAGACAAACACCCAAAGG+AGG 0.614116 7_3:+83971685 None:intergenic
TGGCGGAGACAAACACCCAA+AGG 0.638625 7_3:+83971682 None:intergenic
TAGAGAGCATAAATTATACC+AGG 0.643088 7_3:+83971378 None:intergenic
TATTGTGATTGTGCATACAA+AGG 0.645143 7_3:+83971306 None:intergenic
GACATACAGTGCAGCAACCA+TGG 0.648406 7_3:+83971662 None:intergenic
CAGCAAATTTCACAACGTGA+AGG 0.664232 7_3:+83971733 None:intergenic
ATACAGTGCAGCAACCATGG+CGG 0.788306 7_3:+83971665 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAATTTCATTAAAATCA+TGG + chr7_3:83971400-83971419 None:intergenic 10.0%
!! AAATAATTTCATTAAAATCA+TGG + chr7_3:83971400-83971419 None:intergenic 10.0%
!! ACTAAAAAATCATGAAAAAT+TGG + chr7_3:83971293-83971312 None:intergenic 15.0%
!! AATTCATGTAGAAAATTATA+TGG - chr7_3:83971753-83971772 Msa1131430:exon 15.0%
!! ATCAAATTATGAAAATAAAG+TGG - chr7_3:83971826-83971845 Msa1131430:five_prime_UTR 15.0%
!! ACTAAAAAATCATGAAAAAT+TGG + chr7_3:83971293-83971312 None:intergenic 15.0%
!! AATTCATGTAGAAAATTATA+TGG - chr7_3:83971753-83971772 Msa1131430:exon 15.0%
!! ATCAAATTATGAAAATAAAG+TGG - chr7_3:83971826-83971845 Msa1131430:five_prime_UTR 15.0%
!! AAAGGAAAAAAGAAGTAAAA+GGG + chr7_3:83971256-83971275 None:intergenic 20.0%
!! AAAAGGAAAAAAGAAGTAAA+AGG + chr7_3:83971257-83971276 None:intergenic 20.0%
!!! AATCATGAAAAATTGGATTT+TGG + chr7_3:83971286-83971305 None:intergenic 20.0%
!!! GAAATTATTTTAATTTGTGC+AGG - chr7_3:83971410-83971429 Msa1131430:CDS 20.0%
!! CAAGAATAATAAAATTCAAG+TGG + chr7_3:83971620-83971639 None:intergenic 20.0%
!! AAAGGAAAAAAGAAGTAAAA+GGG + chr7_3:83971256-83971275 None:intergenic 20.0%
!! AAAAGGAAAAAAGAAGTAAA+AGG + chr7_3:83971257-83971276 None:intergenic 20.0%
!!! AATCATGAAAAATTGGATTT+TGG + chr7_3:83971286-83971305 None:intergenic 20.0%
!!! GAAATTATTTTAATTTGTGC+AGG - chr7_3:83971410-83971429 Msa1131430:CDS 20.0%
!! CAAGAATAATAAAATTCAAG+TGG + chr7_3:83971620-83971639 None:intergenic 20.0%
! ATCACGATCAATAAAGATAA+TGG + chr7_3:83971505-83971524 None:intergenic 25.0%
! ATCAGTACTTTGATGAATAT+AGG - chr7_3:83971523-83971542 Msa1131430:intron 25.0%
!! TAAAGTGGTTAGTTATATTG+TGG - chr7_3:83971841-83971860 Msa1131430:five_prime_UTR 25.0%
! ATCACGATCAATAAAGATAA+TGG + chr7_3:83971505-83971524 None:intergenic 25.0%
! ATCAGTACTTTGATGAATAT+AGG - chr7_3:83971523-83971542 Msa1131430:intron 25.0%
! GTGATGATCATCTAAAGTTT+AGG + chr7_3:83971049-83971068 None:intergenic 30.0%
! TTGGATTTTGGATGTGAAAA+AGG + chr7_3:83971274-83971293 None:intergenic 30.0%
GAAGATATCCTAGTATTGTA+AGG + chr7_3:83971458-83971477 None:intergenic 30.0%
! TAGAGAGCATAAATTATACC+AGG + chr7_3:83971480-83971499 None:intergenic 30.0%
TATTGTGATTGTGCATACAA+AGG + chr7_3:83971552-83971571 None:intergenic 30.0%
!! TTCTCTTTGAGATTTGAGTT+AGG - chr7_3:83971650-83971669 Msa1131430:intron 30.0%
! AGATTTGAGTTAGGTCAATT+TGG - chr7_3:83971659-83971678 Msa1131430:intron 30.0%
TTGCATTATTCGTTTCATTG+TGG - chr7_3:83971689-83971708 Msa1131430:CDS 30.0%
! TGATGTGATTGTTGATTGTT+TGG - chr7_3:83971776-83971795 Msa1131430:five_prime_UTR 30.0%
! GTGATGATCATCTAAAGTTT+AGG + chr7_3:83971049-83971068 None:intergenic 30.0%
! TTGGATTTTGGATGTGAAAA+AGG + chr7_3:83971274-83971293 None:intergenic 30.0%
GAAGATATCCTAGTATTGTA+AGG + chr7_3:83971458-83971477 None:intergenic 30.0%
! TAGAGAGCATAAATTATACC+AGG + chr7_3:83971480-83971499 None:intergenic 30.0%
TATTGTGATTGTGCATACAA+AGG + chr7_3:83971552-83971571 None:intergenic 30.0%
!! TTCTCTTTGAGATTTGAGTT+AGG - chr7_3:83971650-83971669 Msa1131430:intron 30.0%
! AGATTTGAGTTAGGTCAATT+TGG - chr7_3:83971659-83971678 Msa1131430:intron 30.0%
TTGCATTATTCGTTTCATTG+TGG - chr7_3:83971689-83971708 Msa1131430:CDS 30.0%
! TGATGTGATTGTTGATTGTT+TGG - chr7_3:83971776-83971795 Msa1131430:five_prime_UTR 30.0%
GATCATCACTTGTTCTTGTT+TGG - chr7_3:83971060-83971079 Msa1131430:three_prime_UTR 35.0%
!!! CATTTTTTCTGGCTTCTTGT+TGG + chr7_3:83971102-83971121 None:intergenic 35.0%
TTACAATCATTGTGAGTGAC+AGG - chr7_3:83971368-83971387 Msa1131430:CDS 35.0%
ACAATACTAGGATATCTTCC+TGG - chr7_3:83971459-83971478 Msa1131430:intron 35.0%
TTGAGTTAGGTCAATTTGGA+TGG - chr7_3:83971663-83971682 Msa1131430:intron 35.0%
ATTGTGGTTTACTCGAAAAC+TGG - chr7_3:83971705-83971724 Msa1131430:CDS 35.0%
GATCATCACTTGTTCTTGTT+TGG - chr7_3:83971060-83971079 Msa1131430:three_prime_UTR 35.0%
!!! CATTTTTTCTGGCTTCTTGT+TGG + chr7_3:83971102-83971121 None:intergenic 35.0%
TTACAATCATTGTGAGTGAC+AGG - chr7_3:83971368-83971387 Msa1131430:CDS 35.0%
ACAATACTAGGATATCTTCC+TGG - chr7_3:83971459-83971478 Msa1131430:intron 35.0%
TTGAGTTAGGTCAATTTGGA+TGG - chr7_3:83971663-83971682 Msa1131430:intron 35.0%
ATTGTGGTTTACTCGAAAAC+TGG - chr7_3:83971705-83971724 Msa1131430:CDS 35.0%
! ACTTGTTCTTGTTTGGATCG+CGG - chr7_3:83971067-83971086 Msa1131430:three_prime_UTR 40.0%
!!! CAACGTGAAGGCATTTTTTC+TGG + chr7_3:83971113-83971132 None:intergenic 40.0%
CAGCAAATTTCACAACGTGA+AGG + chr7_3:83971125-83971144 None:intergenic 40.0%
TGCTTGCTCCTTACAATACT+AGG - chr7_3:83971447-83971466 Msa1131430:intron 40.0%
! ACTTGTTCTTGTTTGGATCG+CGG - chr7_3:83971067-83971086 Msa1131430:three_prime_UTR 40.0%
!!! CAACGTGAAGGCATTTTTTC+TGG + chr7_3:83971113-83971132 None:intergenic 40.0%
CAGCAAATTTCACAACGTGA+AGG + chr7_3:83971125-83971144 None:intergenic 40.0%
TGCTTGCTCCTTACAATACT+AGG - chr7_3:83971447-83971466 Msa1131430:intron 40.0%
! CTTGTTCTTGTTTGGATCGC+GGG - chr7_3:83971068-83971087 Msa1131430:three_prime_UTR 45.0%
TCTCTCTCCATCACTTCCAT+GGG - chr7_3:83971224-83971243 Msa1131430:three_prime_UTR 45.0%
AGTAAAAGGGTAGATCCCCA+TGG + chr7_3:83971243-83971262 None:intergenic 45.0%
! CTTGTTCTTGTTTGGATCGC+GGG - chr7_3:83971068-83971087 Msa1131430:three_prime_UTR 45.0%
TCTCTCTCCATCACTTCCAT+GGG - chr7_3:83971224-83971243 Msa1131430:three_prime_UTR 45.0%
AGTAAAAGGGTAGATCCCCA+TGG + chr7_3:83971243-83971262 None:intergenic 45.0%
GGAGACAAACACCCAAAGGA+GGG + chr7_3:83971172-83971191 None:intergenic 50.0%
ATACAGTGCAGCAACCATGG+CGG + chr7_3:83971193-83971212 None:intergenic 50.0%
GACATACAGTGCAGCAACCA+TGG + chr7_3:83971196-83971215 None:intergenic 50.0%
CTCTCTCTCCATCACTTCCA+TGG - chr7_3:83971223-83971242 Msa1131430:three_prime_UTR 50.0%
CTCTCTCCATCACTTCCATG+GGG - chr7_3:83971225-83971244 Msa1131430:three_prime_UTR 50.0%
GTAGATCCCCATGGAAGTGA+TGG + chr7_3:83971234-83971253 None:intergenic 50.0%
GGAGACAAACACCCAAAGGA+GGG + chr7_3:83971172-83971191 None:intergenic 50.0%
ATACAGTGCAGCAACCATGG+CGG + chr7_3:83971193-83971212 None:intergenic 50.0%
GACATACAGTGCAGCAACCA+TGG + chr7_3:83971196-83971215 None:intergenic 50.0%
CTCTCTCTCCATCACTTCCA+TGG - chr7_3:83971223-83971242 Msa1131430:three_prime_UTR 50.0%
CTCTCTCCATCACTTCCATG+GGG - chr7_3:83971225-83971244 Msa1131430:three_prime_UTR 50.0%
GTAGATCCCCATGGAAGTGA+TGG + chr7_3:83971234-83971253 None:intergenic 50.0%
! GCTGTGTTGCTCCCTCCTTT+GGG - chr7_3:83971158-83971177 Msa1131430:three_prime_UTR 55.0%
CGGAGACAAACACCCAAAGG+AGG + chr7_3:83971173-83971192 None:intergenic 55.0%
TGGCGGAGACAAACACCCAA+AGG + chr7_3:83971176-83971195 None:intergenic 55.0%
TTGGGTGTTTGTCTCCGCCA+TGG - chr7_3:83971176-83971195 Msa1131430:three_prime_UTR 55.0%
! GCTGTGTTGCTCCCTCCTTT+GGG - chr7_3:83971158-83971177 Msa1131430:three_prime_UTR 55.0%
CGGAGACAAACACCCAAAGG+AGG + chr7_3:83971173-83971192 None:intergenic 55.0%
TGGCGGAGACAAACACCCAA+AGG + chr7_3:83971176-83971195 None:intergenic 55.0%
TTGGGTGTTTGTCTCCGCCA+TGG - chr7_3:83971176-83971195 Msa1131430:three_prime_UTR 55.0%
! CGCTGTGTTGCTCCCTCCTT+TGG - chr7_3:83971157-83971176 Msa1131430:three_prime_UTR 60.0%
! CGCTGTGTTGCTCCCTCCTT+TGG - chr7_3:83971157-83971176 Msa1131430:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 83971014 83971863 83971014 ID=Msa1131430;Name=Msa1131430
chr7_3 mRNA 83971014 83971863 83971014 ID=Msa1131430-mRNA-1;Parent=Msa1131430;Name=Msa1131430-mRNA-1;_AED=0.30;_eAED=0.30;_QI=105|1|1|1|1|1|2|288|77
chr7_3 exon 83971014 83971445 83971014 ID=Msa1131430-mRNA-1:exon:24973;Parent=Msa1131430-mRNA-1
chr7_3 exon 83971669 83971863 83971669 ID=Msa1131430-mRNA-1:exon:24972;Parent=Msa1131430-mRNA-1
chr7_3 five_prime_UTR 83971759 83971863 83971759 ID=Msa1131430-mRNA-1:five_prime_utr;Parent=Msa1131430-mRNA-1
chr7_3 CDS 83971669 83971758 83971669 ID=Msa1131430-mRNA-1:cds;Parent=Msa1131430-mRNA-1
chr7_3 CDS 83971302 83971445 83971302 ID=Msa1131430-mRNA-1:cds;Parent=Msa1131430-mRNA-1
chr7_3 three_prime_UTR 83971014 83971301 83971014 ID=Msa1131430-mRNA-1:three_prime_utr;Parent=Msa1131430-mRNA-1
Gene Sequence

>Msa1131430

ATGCCTTCACGTTGTGAAATTTGCTGTGAGATTATGATCGCTGTGTTGCTCCCTCCTTTGGGTGTTTGTCTCCGCCATGGTTGCTGCACTGTTGAATTCATTATTTGCTTGCTCCTTACAATACTAGGATATCTTCCTGGTATAATTTATGCTCTCTATGCCATTATCTTTATTGATCGTGATCAGTACTTTGATGAATATAGGCGTCCTTTGTATGCACAATCACAATACTAG

Protein sequence

>Msa1131430

MPSRCEICCEIMIAVLLPPLGVCLRHGCCTVEFIICLLLTILGYLPGIIYALYAIIFIDRDQYFDEYRRPLYAQSQY*