AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0708410

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0708410 MtrunA17_Chr3g0131301 86.667 90 10 1 50 137 505 594 1.62e-44 157
Msa0708410 MtrunA17_Chr3g0131291 84.444 90 12 1 50 137 431 520 2.39e-41 147
Msa0708410 MtrunA17_Chr4g0046061 96.875 32 1 0 1 32 477 508 2.81e-13 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0708410 AT5G23570.1 64.198 81 29 0 57 137 420 500 4.17e-28 110
Msa0708410 AT5G23570.3 64.198 81 29 0 57 137 420 500 4.17e-28 110
Msa0708410 AT5G23570.4 64.198 81 29 0 57 137 420 500 7.06e-28 109
Msa0708410 AT5G23570.2 64.198 81 29 0 57 137 420 500 7.06e-28 109

Find 96 sgRNAs with CRISPR-Local

Find 251 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATATATTTGACTCTTAATT+TGG 0.170007 4_4:-81964452 None:intergenic
CATCTTCGAGGCATCAATTT+TGG 0.174757 4_4:-81964160 None:intergenic
CATGCAGAGGTTTCTTTATC+TGG 0.200070 4_4:+81963917 Msa0708410:five_prime_UTR
ATCAGAAGTCTGATTCTTAA+TGG 0.218539 4_4:-81963737 None:intergenic
TTGCACTCACATTGTTTCTT+TGG 0.237418 4_4:+81963175 Msa0708410:five_prime_UTR
TGTTGGGGATCGATGTTATT+TGG 0.240823 4_4:+81966896 Msa0708410:CDS
TAACATACTTTCTTGGGTTA+TGG 0.258542 4_4:-81964580 None:intergenic
AAATCAAATGAGACAAAATT+TGG 0.262441 4_4:+81964686 Msa0708410:five_prime_UTR
AAACCTTAACATAGTAATTA+TGG 0.266078 4_4:-81963267 None:intergenic
CATTGTTTCTTTGGAGAGTC+AGG 0.303863 4_4:+81963184 Msa0708410:five_prime_UTR
TTTCTTGGGTTATGGATCTC+TGG 0.339889 4_4:-81964572 None:intergenic
GACAAAATTTGGAAGCAAAA+TGG 0.343517 4_4:+81964697 Msa0708410:exon
ACATAGTAATTATGGACATT+TGG 0.352634 4_4:-81963259 None:intergenic
TTCCAGAGACAAATAATATT+AGG 0.355472 4_4:-81963232 None:intergenic
TGTCCATAATTACTATGTTA+AGG 0.364571 4_4:+81963264 Msa0708410:five_prime_UTR
GTAAGCATAACATACTTTCT+TGG 0.366097 4_4:-81964587 None:intergenic
AACAGCTTTGGAGTGTCATT+TGG 0.369013 4_4:+81964218 Msa0708410:five_prime_UTR
AGAAAATAAAAGAACAGCTT+TGG 0.376078 4_4:+81964206 Msa0708410:five_prime_UTR
TAAGCATAACATACTTTCTT+GGG 0.388461 4_4:-81964586 None:intergenic
GATGTAGTTGGAGGCATGTT+GGG 0.390830 4_4:+81966880 Msa0708410:CDS
AAATCTCTTGAGGAATCCTT+TGG 0.392718 4_4:+81965719 Msa0708410:CDS
CCAGCCAACTTGCCTGCACT+TGG 0.394445 4_4:-81964991 None:intergenic
GGGATCGATGTTATTTGGTC+TGG 0.403261 4_4:+81966901 Msa0708410:CDS
TTCCTAATATTATTTGTCTC+TGG 0.408290 4_4:+81963230 Msa0708410:five_prime_UTR
GAAACCTCTGCATGCTGCTC+TGG 0.426850 4_4:-81963908 None:intergenic
TCTTCAAATAAAGAAGTTGC+TGG 0.427490 4_4:-81965667 None:intergenic
TCTTTAATAAAACCTATGTC+AGG 0.437390 4_4:-81963087 None:intergenic
TGAAAGAGTAACATCCTGTC+TGG 0.445042 4_4:-81963683 None:intergenic
AATAACAAGTATGAAAGAAA+TGG 0.445451 4_4:+81963768 Msa0708410:five_prime_UTR
GGTGGCAATCGACAGCTCTT+CGG 0.448518 4_4:+81963618 Msa0708410:intron
GTTGCTTGAATTCATCTTCG+AGG 0.451593 4_4:-81964172 None:intergenic
TTTCAGCCTTGTCGTCAAAT+CGG 0.455474 4_4:+81966832 Msa0708410:CDS
AGAAACAATGTGAGTGCAAA+GGG 0.464247 4_4:-81963171 None:intergenic
CACAATGTGATTCTCCTCCA+TGG 0.469410 4_4:-81965768 None:intergenic
CCAAGTGCAGGCAAGTTGGC+TGG 0.478635 4_4:+81964991 Msa0708410:CDS
TTCGTTACTTTGCTAGAACC+TGG 0.478950 4_4:-81964734 None:intergenic
GTTAGAGAAGCAGTTAATGT+AGG 0.480862 4_4:+81964070 Msa0708410:five_prime_UTR
AGATGTAGTTGGAGGCATGT+TGG 0.492217 4_4:+81966879 Msa0708410:CDS
TCGTTACTTTGCTAGAACCT+GGG 0.502319 4_4:-81964733 None:intergenic
AAGGATGCACCAGCGTTGCT+TGG 0.504106 4_4:+81964755 Msa0708410:CDS
ACTTAGGAGACAATTTCAAC+TGG 0.507613 4_4:+81966650 Msa0708410:intron
TCCTGTCTGGAAACTATTGA+AGG 0.508364 4_4:-81963670 None:intergenic
GGAAACTATTGAAGGCCTCC+AGG 0.511331 4_4:-81963662 None:intergenic
TACCATGTTCAGATGGCAAC+TGG 0.511457 4_4:+81966805 Msa0708410:intron
ATGGTAAGCGGTTCACTCCC+AGG 0.519431 4_4:+81964716 Msa0708410:CDS
TTTGATGCACAACCATCTCT+TGG 0.519566 4_4:-81965622 None:intergenic
CCTTGTCGTCAAATCGGAGC+TGG 0.521601 4_4:+81966838 Msa0708410:CDS
GTCATTTGGCTGCAGCAAGC+AGG 0.524435 4_4:+81964232 Msa0708410:five_prime_UTR
AATCACTTTGACCTACAGAC+TGG 0.529057 4_4:-81963867 None:intergenic
GTTTCTTTGGAGAGTCAGGT+TGG 0.536553 4_4:+81963188 Msa0708410:five_prime_UTR
GCCTTCAATAGTTTCCAGAC+AGG 0.540883 4_4:+81963669 Msa0708410:five_prime_UTR
TCTTTGGAGAGTCAGGTTGG+AGG 0.542497 4_4:+81963191 Msa0708410:five_prime_UTR
AACAGAATCGAAATCTGAGC+AGG 0.545749 4_4:+81963825 Msa0708410:five_prime_UTR
TGCATCTGTGTAGATGTAGT+TGG 0.547258 4_4:+81966868 Msa0708410:CDS
AAAGAATAATCGTCAACAGC+GGG 0.547306 4_4:+81964506 Msa0708410:five_prime_UTR
TCGACAGCTCTTCGGATGCA+TGG 0.547576 4_4:+81963626 Msa0708410:intron
CTGATTCAGTGGTATGAGGT+GGG 0.550804 4_4:-81964039 None:intergenic
CCATCTGATTCAGTGGTATG+AGG 0.554125 4_4:-81964043 None:intergenic
CATGGCAACTAAAGAAGACC+TGG 0.556551 4_4:+81963644 Msa0708410:five_prime_UTR
TTAGATGCTACACCAAGTGC+AGG 0.557009 4_4:+81964979 Msa0708410:CDS
TCTTTATTTGAAGAACAAGA+TGG 0.560615 4_4:+81965676 Msa0708410:CDS
GATTCTTAATGGCCATGTCA+AGG 0.573422 4_4:-81963726 None:intergenic
AACAATGCCATCTGATTCAG+TGG 0.575477 4_4:-81964050 None:intergenic
ATCAAGAATTATATACATGT+AGG 0.575586 4_4:+81963137 Msa0708410:five_prime_UTR
TACACCAAGTGCAGGCAAGT+TGG 0.575718 4_4:+81964987 Msa0708410:CDS
AAGGTATCTTCCCAGTCTGT+AGG 0.576262 4_4:+81963856 Msa0708410:five_prime_UTR
CTGATTCTGTAGGTAAAACA+AGG 0.579636 4_4:+81965576 Msa0708410:intron
TTCCTCTGAGAACCTTGACA+TGG 0.579915 4_4:+81963714 Msa0708410:five_prime_UTR
GCATGAAGAAATAAAAGAAG+AGG 0.582341 4_4:+81965811 Msa0708410:CDS
GAAACATTCAAAATCTCTTG+AGG 0.583454 4_4:+81965709 Msa0708410:CDS
TCATGGTTAGCATTCAATGA+AGG 0.584619 4_4:+81963315 Msa0708410:five_prime_UTR
AAGAAACAATGTGAGTGCAA+AGG 0.590446 4_4:-81963172 None:intergenic
ATCTTAAAAGTTGTAATACC+AGG 0.590739 4_4:+81964789 Msa0708410:CDS
TCTGATTCAGTGGTATGAGG+TGG 0.599621 4_4:-81964040 None:intergenic
CAAGGCTCAAGTATGAAATG+AGG 0.603974 4_4:+81965594 Msa0708410:CDS
ATGTAGTTGGAGGCATGTTG+GGG 0.605055 4_4:+81966881 Msa0708410:CDS
CGAGAAACTGCGTAAAACCA+TGG 0.608482 4_4:+81965751 Msa0708410:CDS
TTTCTCGGTCACTATACCAA+AGG 0.611686 4_4:-81965735 None:intergenic
CTTTAATAAAACCTATGTCA+GGG 0.613390 4_4:-81963086 None:intergenic
ATCTGAGCAGGTTATCAACA+AGG 0.618921 4_4:+81963837 Msa0708410:five_prime_UTR
ATCACTTTGACCTACAGACT+GGG 0.619114 4_4:-81963866 None:intergenic
CCTCATACCACTGAATCAGA+TGG 0.624063 4_4:+81964043 Msa0708410:five_prime_UTR
TTTGGAAGCAAAATGGTAAG+CGG 0.624962 4_4:+81964704 Msa0708410:exon
ATCTCCAGAGCAGCATGCAG+AGG 0.628142 4_4:+81963904 Msa0708410:five_prime_UTR
TGGAGGAGAATCACATTGTG+AGG 0.630076 4_4:+81965771 Msa0708410:CDS
AATGAGGTTCTACCAAGAGA+TGG 0.634854 4_4:+81965610 Msa0708410:CDS
CCAGCTCCGATTTGACGACA+AGG 0.635649 4_4:-81966838 None:intergenic
CCTTCTGCAAAATAGAGCAG+TGG 0.647097 4_4:+81966679 Msa0708410:CDS
TGATTCAGTGGTATGAGGTG+GGG 0.650629 4_4:-81964038 None:intergenic
ATCTGTGTAGATGTAGTTGG+AGG 0.654939 4_4:+81966871 Msa0708410:CDS
AGGAGAATCACATTGTGAGG+CGG 0.677580 4_4:+81965774 Msa0708410:CDS
AGGTTCTAGCAAAGTAACGA+AGG 0.677876 4_4:+81964736 Msa0708410:CDS
TAAAGAATAATCGTCAACAG+CGG 0.683336 4_4:+81964505 Msa0708410:five_prime_UTR
GGCCATGTCAAGGTTCTCAG+AGG 0.691240 4_4:-81963716 None:intergenic
GGCAACTAAAGAAGACCTGG+AGG 0.707637 4_4:+81963647 Msa0708410:five_prime_UTR
GAAACTGCGTAAAACCATGG+AGG 0.748048 4_4:+81965754 Msa0708410:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTAGTTTATATTCAAGTAA+TGG - chr4_4:81963497-81963516 None:intergenic 15.0%
!! AATATATTTGACTCTTAATT+TGG - chr4_4:81964455-81964474 None:intergenic 15.0%
!! ATAAATGTCCAATATATAAT+GGG - chr4_4:81966244-81966263 None:intergenic 15.0%
!! TTTATAATATAAACACAAAC+TGG - chr4_4:81966380-81966399 None:intergenic 15.0%
!!! GATTTTCATATTTATTTCAA+TGG + chr4_4:81966570-81966589 Msa0708410:intron 15.0%
!! ATCAAGAATTATATACATGT+AGG + chr4_4:81963137-81963156 Msa0708410:five_prime_UTR 20.0%
!! AAACCTTAACATAGTAATTA+TGG - chr4_4:81963270-81963289 None:intergenic 20.0%
!!! TTACTATGTTAAGGTTTTAT+TGG + chr4_4:81963273-81963292 Msa0708410:five_prime_UTR 20.0%
!! TGAATGTGAATTAAAAGAAT+AGG - chr4_4:81963365-81963384 None:intergenic 20.0%
!! AATAACAAGTATGAAAGAAA+TGG + chr4_4:81963768-81963787 Msa0708410:five_prime_UTR 20.0%
!!! CTTCAGATAAGTTTTTTTTT+TGG - chr4_4:81963795-81963814 None:intergenic 20.0%
!! TTAAAAAGAGGTTAATGATA+AGG + chr4_4:81963967-81963986 Msa0708410:five_prime_UTR 20.0%
!! AAATCAAATGAGACAAAATT+TGG + chr4_4:81964686-81964705 Msa0708410:five_prime_UTR 20.0%
!! CAAAAAATGTTAATATTTCG+AGG + chr4_4:81965082-81965101 Msa0708410:intron 20.0%
!!! ACTCTTATAATTTTTAGTTC+TGG - chr4_4:81965147-81965166 None:intergenic 20.0%
!! ATGAGTTAGCAAATAAATTT+CGG + chr4_4:81965276-81965295 Msa0708410:intron 20.0%
!! TTACTTGAAGCATATTAATT+AGG + chr4_4:81965854-81965873 Msa0708410:intron 20.0%
!! TCTCCTTATAAAATTATTTG+TGG + chr4_4:81965900-81965919 Msa0708410:intron 20.0%
!!! TGTCCACAAATAATTTTATA+AGG - chr4_4:81965906-81965925 None:intergenic 20.0%
!!! TTGTTTGCTATTAAGAATAT+GGG - chr4_4:81966190-81966209 None:intergenic 20.0%
!!! TTTGTTTGCTATTAAGAATA+TGG - chr4_4:81966191-81966210 None:intergenic 20.0%
!! GATAAATGTCCAATATATAA+TGG - chr4_4:81966245-81966264 None:intergenic 20.0%
!!! ATTTTTCTTTTTTTCTCCAA+AGG - chr4_4:81966540-81966559 None:intergenic 20.0%
!!! CTTAACTGTATTATTTTTGT+TGG + chr4_4:81966627-81966646 Msa0708410:intron 20.0%
! CTTTAATAAAACCTATGTCA+GGG - chr4_4:81963089-81963108 None:intergenic 25.0%
! TCTTTAATAAAACCTATGTC+AGG - chr4_4:81963090-81963109 None:intergenic 25.0%
! TTCCTAATATTATTTGTCTC+TGG + chr4_4:81963230-81963249 Msa0708410:five_prime_UTR 25.0%
! TTCCAGAGACAAATAATATT+AGG - chr4_4:81963235-81963254 None:intergenic 25.0%
! ACATAGTAATTATGGACATT+TGG - chr4_4:81963262-81963281 None:intergenic 25.0%
! TGTCCATAATTACTATGTTA+AGG + chr4_4:81963264-81963283 Msa0708410:five_prime_UTR 25.0%
! ATCATAAATGGGATTTACAA+AGG - chr4_4:81963469-81963488 None:intergenic 25.0%
! TAATGGAAGGAATCATAAAT+GGG - chr4_4:81963480-81963499 None:intergenic 25.0%
!! GTTTATATTCAAGTAATGGA+AGG - chr4_4:81963493-81963512 None:intergenic 25.0%
! TTCCAAGAAAATTCATGATA+GGG - chr4_4:81963539-81963558 None:intergenic 25.0%
! TTTCCAAGAAAATTCATGAT+AGG - chr4_4:81963540-81963559 None:intergenic 25.0%
! AGAAAATAAAAGAACAGCTT+TGG + chr4_4:81964206-81964225 Msa0708410:five_prime_UTR 25.0%
! GCAAAGATGTGATTATAAAA+AGG - chr4_4:81964272-81964291 None:intergenic 25.0%
!!! AATGTGGTTTTATCAACTAT+GGG - chr4_4:81964411-81964430 None:intergenic 25.0%
! TAAGCATAACATACTTTCTT+GGG - chr4_4:81964589-81964608 None:intergenic 25.0%
! ACCTTAACAATTGTATTTGA+TGG + chr4_4:81964608-81964627 Msa0708410:intron 25.0%
! CCTTAACAATTGTATTTGAT+GGG + chr4_4:81964609-81964628 Msa0708410:intron 25.0%
! ATCTTAAAAGTTGTAATACC+AGG + chr4_4:81964789-81964808 Msa0708410:CDS 25.0%
! ATGAGCATATATATATTACC+TGG - chr4_4:81964810-81964829 None:intergenic 25.0%
! AGGTAATATATATATGCTCA+TGG + chr4_4:81964809-81964828 Msa0708410:intron 25.0%
! AACAATCACATTCATTACTT+AGG - chr4_4:81964923-81964942 None:intergenic 25.0%
! AATGTTAATATTTCGAGGTA+AGG + chr4_4:81965087-81965106 Msa0708410:intron 25.0%
! AAATTATAAGAGTCCAGATA+TGG + chr4_4:81965154-81965173 Msa0708410:intron 25.0%
!! ATATGATTTTATGATACTGC+AGG - chr4_4:81965203-81965222 None:intergenic 25.0%
! TGCAGTATCATAAAATCATA+TGG + chr4_4:81965202-81965221 Msa0708410:intron 25.0%
! GCAGTATCATAAAATCATAT+GGG + chr4_4:81965203-81965222 Msa0708410:intron 25.0%
!! TCTAGTTTGAATAAAAGCAT+AGG - chr4_4:81965238-81965257 None:intergenic 25.0%
! TCTTTGTACTTTCACTATTT+AGG + chr4_4:81965468-81965487 Msa0708410:intron 25.0%
! AGAATCAGATTAAAAGAGAA+AGG - chr4_4:81965564-81965583 None:intergenic 25.0%
!! TCTTTATTTGAAGAACAAGA+TGG + chr4_4:81965676-81965695 Msa0708410:CDS 25.0%
! TAAGCAATATAAAGTGAAGT+TGG - chr4_4:81966611-81966630 None:intergenic 25.0%
!!! GTATTATTTTTGTTGGACTT+AGG + chr4_4:81966634-81966653 Msa0708410:intron 25.0%
! AAATTGCAATTGTCTATGAA+CGG - chr4_4:81966754-81966773 None:intergenic 25.0%
!!! ATAGTTTTAAGGCTATATCA+TGG + chr4_4:81967059-81967078 Msa0708410:CDS 25.0%
!!! TAGTTTTAAGGCTATATCAT+GGG + chr4_4:81967060-81967079 Msa0708410:CDS 25.0%
! TTTTTGATACGTGATCATCA+TGG + chr4_4:81963298-81963317 Msa0708410:five_prime_UTR 30.0%
!!! ATGAAGGCATTTTGTTTGAT+TGG + chr4_4:81963331-81963350 Msa0708410:five_prime_UTR 30.0%
! GTAATGGAAGGAATCATAAA+TGG - chr4_4:81963481-81963500 None:intergenic 30.0%
! ATGATAGGGTAATGCTATTT+GGG - chr4_4:81963525-81963544 None:intergenic 30.0%
! TACCCTATCATGAATTTTCT+TGG + chr4_4:81963534-81963553 Msa0708410:intron 30.0%
GGAGAAACAAATTGTAACTT+CGG - chr4_4:81963596-81963615 None:intergenic 30.0%
ATCAGAAGTCTGATTCTTAA+TGG - chr4_4:81963740-81963759 None:intergenic 30.0%
TGTTGCATCTGATTAAAAAG+AGG + chr4_4:81963955-81963974 Msa0708410:five_prime_UTR 30.0%
!!! ATGTGGTTTTATCAACTATG+GGG - chr4_4:81964410-81964429 None:intergenic 30.0%
!!! CAATGTGGTTTTATCAACTA+TGG - chr4_4:81964412-81964431 None:intergenic 30.0%
TAAAGAATAATCGTCAACAG+CGG + chr4_4:81964505-81964524 Msa0708410:five_prime_UTR 30.0%
TAACATACTTTCTTGGGTTA+TGG - chr4_4:81964583-81964602 None:intergenic 30.0%
GTAAGCATAACATACTTTCT+TGG - chr4_4:81964590-81964609 None:intergenic 30.0%
CCCATCAAATACAATTGTTA+AGG - chr4_4:81964612-81964631 None:intergenic 30.0%
GACAAAATTTGGAAGCAAAA+TGG + chr4_4:81964697-81964716 Msa0708410:exon 30.0%
! CTCTTTTAATCTGATTCTGT+AGG + chr4_4:81965566-81965585 Msa0708410:intron 30.0%
TCTTCAAATAAAGAAGTTGC+TGG - chr4_4:81965670-81965689 None:intergenic 30.0%
GAAACATTCAAAATCTCTTG+AGG + chr4_4:81965709-81965728 Msa0708410:CDS 30.0%
GCATGAAGAAATAAAAGAAG+AGG + chr4_4:81965811-81965830 Msa0708410:CDS 30.0%
TTATTTGTGGACAATCAAGT+TGG + chr4_4:81965913-81965932 Msa0708410:intron 30.0%
TGCAATATCTAAACGTTTCA+TGG - chr4_4:81966158-81966177 None:intergenic 30.0%
GCTATTAAGAATATGGGAAA+AGG - chr4_4:81966184-81966203 None:intergenic 30.0%
TATATTGGACATTTATCCAG+AGG + chr4_4:81966248-81966267 Msa0708410:intron 30.0%
TCTTGGAAAGATTTGGATTT+GGG + chr4_4:81966305-81966324 Msa0708410:intron 30.0%
!! GTACTTATGTTTTGTTGTAG+AGG + chr4_4:81966332-81966351 Msa0708410:intron 30.0%
ATGGTCAATGTTGAAATTAC+CGG - chr4_4:81966432-81966451 None:intergenic 30.0%
GTAATGAAATAACACCCAAT+GGG - chr4_4:81966718-81966737 None:intergenic 30.0%
TGTAATGAAATAACACCCAA+TGG - chr4_4:81966719-81966738 None:intergenic 30.0%
TCAAAGATCAAAACCAACAT+AGG - chr4_4:81966995-81967014 None:intergenic 30.0%
! TAAGTTGTGCAAAATTTTGC+AGG + chr4_4:81967020-81967039 Msa0708410:intron 30.0%
!! TAGTGAGGCTAATAGTTTTA+AGG + chr4_4:81967048-81967067 Msa0708410:CDS 30.0%
AGAAACAATGTGAGTGCAAA+GGG - chr4_4:81963174-81963193 None:intergenic 35.0%
AAGAAACAATGTGAGTGCAA+AGG - chr4_4:81963175-81963194 None:intergenic 35.0%
! TTGCACTCACATTGTTTCTT+TGG + chr4_4:81963175-81963194 Msa0708410:five_prime_UTR 35.0%
TCATGGTTAGCATTCAATGA+AGG + chr4_4:81963315-81963334 Msa0708410:five_prime_UTR 35.0%
! TGATAGGGTAATGCTATTTG+GGG - chr4_4:81963524-81963543 None:intergenic 35.0%
CATGATAGGGTAATGCTATT+TGG - chr4_4:81963526-81963545 None:intergenic 35.0%
ATTGTAACTTCGGCAATAGA+GGG - chr4_4:81963586-81963605 None:intergenic 35.0%
AATTGTAACTTCGGCAATAG+AGG - chr4_4:81963587-81963606 None:intergenic 35.0%
! AGTTACAATTTGTTTCTCCC+TGG + chr4_4:81963597-81963616 Msa0708410:intron 35.0%
GTTAGAGAAGCAGTTAATGT+AGG + chr4_4:81964070-81964089 Msa0708410:five_prime_UTR 35.0%
!!! AATCACTCTTTTCTGGTTGA+CGG - chr4_4:81964122-81964141 None:intergenic 35.0%
! TGACTTGAATCACTCTTTTC+TGG - chr4_4:81964129-81964148 None:intergenic 35.0%
AAAGAATAATCGTCAACAGC+GGG + chr4_4:81964506-81964525 Msa0708410:five_prime_UTR 35.0%
TTTGGAAGCAAAATGGTAAG+CGG + chr4_4:81964704-81964723 Msa0708410:exon 35.0%
ATGCTATCTTTGTGGAAGTT+AGG + chr4_4:81964888-81964907 Msa0708410:intron 35.0%
TTCACGAAGATGTAAGTAGA+AGG + chr4_4:81965028-81965047 Msa0708410:intron 35.0%
ACTTGCAAAACAAAAGTCAG+CGG - chr4_4:81965119-81965138 None:intergenic 35.0%
!!! CTGACTTTTGTTTTGCAAGT+AGG + chr4_4:81965119-81965138 Msa0708410:intron 35.0%
! AGTCCAGATATGGATTTTTG+TGG + chr4_4:81965164-81965183 Msa0708410:intron 35.0%
ATACTTTACTTCCACCATTC+TGG - chr4_4:81965528-81965547 None:intergenic 35.0%
CTGATTCTGTAGGTAAAACA+AGG + chr4_4:81965576-81965595 Msa0708410:intron 35.0%
AAATCTCTTGAGGAATCCTT+TGG + chr4_4:81965719-81965738 Msa0708410:CDS 35.0%
! TGCCTGCAAAAATGTGATTT+TGG + chr4_4:81966060-81966079 Msa0708410:intron 35.0%
! GCCTGCAAAAATGTGATTTT+GGG + chr4_4:81966061-81966080 Msa0708410:intron 35.0%
GCAAACAAATCAATGAGCAA+TGG + chr4_4:81966202-81966221 Msa0708410:intron 35.0%
!! ATGTCCAATATATAATGGGC+TGG - chr4_4:81966240-81966259 None:intergenic 35.0%
TCAAAATGAAAGCAGAGTCT+TGG + chr4_4:81966288-81966307 Msa0708410:intron 35.0%
GTCTTGGAAAGATTTGGATT+TGG + chr4_4:81966304-81966323 Msa0708410:intron 35.0%
AATTTCTCTATCATGTGTCC+CGG + chr4_4:81966410-81966429 Msa0708410:intron 35.0%
TGGTCAATGTTGAAATTACC+GGG - chr4_4:81966431-81966450 None:intergenic 35.0%
AGGCTGATAAGATTTGTGTT+AGG - chr4_4:81966520-81966539 None:intergenic 35.0%
ACACAAATCTTATCAGCCTT+TGG + chr4_4:81966521-81966540 Msa0708410:intron 35.0%
ACTTAGGAGACAATTTCAAC+TGG + chr4_4:81966650-81966669 Msa0708410:intron 35.0%
TTTATGTCTTCTGCATTGCT+CGG + chr4_4:81966771-81966790 Msa0708410:intron 35.0%
AACCTTCTCAAAATAACCAC+TGG - chr4_4:81966970-81966989 None:intergenic 35.0%
! ATTTTGCAGGATACATAGTG+AGG + chr4_4:81967033-81967052 Msa0708410:intron 35.0%
! CATTGTTTCTTTGGAGAGTC+AGG + chr4_4:81963184-81963203 Msa0708410:five_prime_UTR 40.0%
! TACAATTTGTTTCTCCCTGG+TGG + chr4_4:81963600-81963619 Msa0708410:intron 40.0%
TCCTGTCTGGAAACTATTGA+AGG - chr4_4:81963673-81963692 None:intergenic 40.0%
TGAAAGAGTAACATCCTGTC+TGG - chr4_4:81963686-81963705 None:intergenic 40.0%
GATTCTTAATGGCCATGTCA+AGG - chr4_4:81963729-81963748 None:intergenic 40.0%
AACAGAATCGAAATCTGAGC+AGG + chr4_4:81963825-81963844 Msa0708410:five_prime_UTR 40.0%
ATCTGAGCAGGTTATCAACA+AGG + chr4_4:81963837-81963856 Msa0708410:five_prime_UTR 40.0%
ATCACTTTGACCTACAGACT+GGG - chr4_4:81963869-81963888 None:intergenic 40.0%
AATCACTTTGACCTACAGAC+TGG - chr4_4:81963870-81963889 None:intergenic 40.0%
CATGCAGAGGTTTCTTTATC+TGG + chr4_4:81963917-81963936 Msa0708410:five_prime_UTR 40.0%
AACAATGCCATCTGATTCAG+TGG - chr4_4:81964053-81964072 None:intergenic 40.0%
!! CATCTTCGAGGCATCAATTT+TGG - chr4_4:81964163-81964182 None:intergenic 40.0%
GTTGCTTGAATTCATCTTCG+AGG - chr4_4:81964175-81964194 None:intergenic 40.0%
!! AACAGCTTTGGAGTGTCATT+TGG + chr4_4:81964218-81964237 Msa0708410:five_prime_UTR 40.0%
CTCTGCATCTTTCATCAATG+TGG - chr4_4:81964427-81964446 None:intergenic 40.0%
TTTCTTGGGTTATGGATCTC+TGG - chr4_4:81964575-81964594 None:intergenic 40.0%
ATTTGATGCTGTTCGTGATG+TGG - chr4_4:81964670-81964689 None:intergenic 40.0%
! TCGTTACTTTGCTAGAACCT+GGG - chr4_4:81964736-81964755 None:intergenic 40.0%
! TTCGTTACTTTGCTAGAACC+TGG - chr4_4:81964737-81964756 None:intergenic 40.0%
! AGGTTCTAGCAAAGTAACGA+AGG + chr4_4:81964736-81964755 Msa0708410:CDS 40.0%
CTGCAAAGATGCTATCTTTG+TGG + chr4_4:81964880-81964899 Msa0708410:intron 40.0%
CGAAGATGTAAGTAGAAGGA+AGG + chr4_4:81965032-81965051 Msa0708410:intron 40.0%
CAGCCACAAAAATCCATATC+TGG - chr4_4:81965170-81965189 None:intergenic 40.0%
! CAGATATGGATTTTTGTGGC+TGG + chr4_4:81965168-81965187 Msa0708410:intron 40.0%
TGAGAAAAGTTGCCATGTTC+TGG - chr4_4:81965407-81965426 None:intergenic 40.0%
CAAGGCTCAAGTATGAAATG+AGG + chr4_4:81965594-81965613 Msa0708410:CDS 40.0%
AATGAGGTTCTACCAAGAGA+TGG + chr4_4:81965610-81965629 Msa0708410:CDS 40.0%
TTTGATGCACAACCATCTCT+TGG - chr4_4:81965625-81965644 None:intergenic 40.0%
TTTCTCGGTCACTATACCAA+AGG - chr4_4:81965738-81965757 None:intergenic 40.0%
!! CATGGTTTTACGCAGTTTCT+CGG - chr4_4:81965753-81965772 None:intergenic 40.0%
!! TTTGTGGACAATCAAGTTGG+AGG + chr4_4:81965916-81965935 Msa0708410:intron 40.0%
!! ATCAAGTTGGAGGTAGGATA+TGG + chr4_4:81965926-81965945 Msa0708410:intron 40.0%
! GCCCAAAATCACATTTTTGC+AGG - chr4_4:81966065-81966084 None:intergenic 40.0%
TCAGAGTGGAGGAGATTAAT+GGG - chr4_4:81966087-81966106 None:intergenic 40.0%
! TACTTTGAAAGTCAGAGTGG+AGG - chr4_4:81966098-81966117 None:intergenic 40.0%
! GTCTACTTTGAAAGTCAGAG+TGG - chr4_4:81966101-81966120 None:intergenic 40.0%
AATGAGCAATGGTAAAGAGC+TGG + chr4_4:81966213-81966232 Msa0708410:intron 40.0%
TGGTCCAGCCCATTATATAT+TGG + chr4_4:81966233-81966252 Msa0708410:intron 40.0%
AGCAGAGTCTTGGAAAGATT+TGG + chr4_4:81966298-81966317 Msa0708410:intron 40.0%
GTAAAAAGTTCCCTCCCATT+GGG + chr4_4:81966701-81966720 Msa0708410:intron 40.0%
TGAAATAACACCCAATGGGA+GGG - chr4_4:81966714-81966733 None:intergenic 40.0%
ATGAAATAACACCCAATGGG+AGG - chr4_4:81966715-81966734 None:intergenic 40.0%
! TTTCAGCCTTGTCGTCAAAT+CGG + chr4_4:81966832-81966851 Msa0708410:CDS 40.0%
TGCATCTGTGTAGATGTAGT+TGG + chr4_4:81966868-81966887 Msa0708410:CDS 40.0%
ATCTGTGTAGATGTAGTTGG+AGG + chr4_4:81966871-81966890 Msa0708410:CDS 40.0%
TGTTGGGGATCGATGTTATT+TGG + chr4_4:81966896-81966915 Msa0708410:CDS 40.0%
!!! CACCAGTGGTTATTTTGAGA+AGG + chr4_4:81966965-81966984 Msa0708410:intron 40.0%
TTGAGAAGGTTGTCCTATGT+TGG + chr4_4:81966979-81966998 Msa0708410:intron 40.0%
! GTTTCTTTGGAGAGTCAGGT+TGG + chr4_4:81963188-81963207 Msa0708410:five_prime_UTR 45.0%
!!! AGTCAGGTTGGAGGTTTTTG+AGG + chr4_4:81963200-81963219 Msa0708410:five_prime_UTR 45.0%
CATGGCAACTAAAGAAGACC+TGG + chr4_4:81963644-81963663 Msa0708410:five_prime_UTR 45.0%
GCCTTCAATAGTTTCCAGAC+AGG + chr4_4:81963669-81963688 Msa0708410:five_prime_UTR 45.0%
! TTCCTCTGAGAACCTTGACA+TGG + chr4_4:81963714-81963733 Msa0708410:five_prime_UTR 45.0%
! AAGGTATCTTCCCAGTCTGT+AGG + chr4_4:81963856-81963875 Msa0708410:five_prime_UTR 45.0%
TGATTCAGTGGTATGAGGTG+GGG - chr4_4:81964041-81964060 None:intergenic 45.0%
CTGATTCAGTGGTATGAGGT+GGG - chr4_4:81964042-81964061 None:intergenic 45.0%
TCTGATTCAGTGGTATGAGG+TGG - chr4_4:81964043-81964062 None:intergenic 45.0%
CCATCTGATTCAGTGGTATG+AGG - chr4_4:81964046-81964065 None:intergenic 45.0%
CCTCATACCACTGAATCAGA+TGG + chr4_4:81964043-81964062 Msa0708410:five_prime_UTR 45.0%
GCTGAAACTCCTATTCTGCA+AGG + chr4_4:81964319-81964338 Msa0708410:intron 45.0%
TCCAAATTCCGATCATGACC+AGG + chr4_4:81964946-81964965 Msa0708410:intron 45.0%
CCAAATTCCGATCATGACCA+GGG + chr4_4:81964947-81964966 Msa0708410:intron 45.0%
CAAATTCCGATCATGACCAG+GGG + chr4_4:81964948-81964967 Msa0708410:intron 45.0%
AAATTCCGATCATGACCAGG+GGG + chr4_4:81964949-81964968 Msa0708410:intron 45.0%
TTAGATGCTACACCAAGTGC+AGG + chr4_4:81964979-81964998 Msa0708410:CDS 45.0%
AAGGCCATGTTTCCTGAAAC+TGG + chr4_4:81965051-81965070 Msa0708410:intron 45.0%
CATGCCAGTTTCAGGAAACA+TGG - chr4_4:81965058-81965077 None:intergenic 45.0%
TTTGCTGACATGCCAGTTTC+AGG - chr4_4:81965066-81965085 None:intergenic 45.0%
CTCACCTTACAACCAGAACA+TGG + chr4_4:81965392-81965411 Msa0708410:intron 45.0%
! GTTGCCATGTTCTGGTTGTA+AGG - chr4_4:81965399-81965418 None:intergenic 45.0%
GTTTGATGTCAACCAGTTGC+AGG + chr4_4:81965493-81965512 Msa0708410:intron 45.0%
TTACTTCCACCATTCTGGCA+TGG - chr4_4:81965523-81965542 None:intergenic 45.0%
CGAGAAACTGCGTAAAACCA+TGG + chr4_4:81965751-81965770 Msa0708410:CDS 45.0%
GAAACTGCGTAAAACCATGG+AGG + chr4_4:81965754-81965773 Msa0708410:CDS 45.0%
CACAATGTGATTCTCCTCCA+TGG - chr4_4:81965771-81965790 None:intergenic 45.0%
TGGAGGAGAATCACATTGTG+AGG + chr4_4:81965771-81965790 Msa0708410:CDS 45.0%
AGGAGAATCACATTGTGAGG+CGG + chr4_4:81965774-81965793 Msa0708410:CDS 45.0%
!! TGGACAATCAAGTTGGAGGT+AGG + chr4_4:81965920-81965939 Msa0708410:intron 45.0%
GTCAGAGTGGAGGAGATTAA+TGG - chr4_4:81966088-81966107 None:intergenic 45.0%
ACACTGCAAAACAACACCTC+TGG - chr4_4:81966267-81966286 None:intergenic 45.0%
! CCACTGCTCTATTTTGCAGA+AGG - chr4_4:81966682-81966701 None:intergenic 45.0%
CCTTCTGCAAAATAGAGCAG+TGG + chr4_4:81966679-81966698 Msa0708410:CDS 45.0%
GGTAAAAAGTTCCCTCCCAT+TGG + chr4_4:81966700-81966719 Msa0708410:intron 45.0%
GGTTGCATACCATGTTCAGA+TGG + chr4_4:81966798-81966817 Msa0708410:intron 45.0%
TACCATGTTCAGATGGCAAC+TGG + chr4_4:81966805-81966824 Msa0708410:intron 45.0%
! AGATGTAGTTGGAGGCATGT+TGG + chr4_4:81966879-81966898 Msa0708410:CDS 45.0%
! GATGTAGTTGGAGGCATGTT+GGG + chr4_4:81966880-81966899 Msa0708410:CDS 45.0%
! ATGTAGTTGGAGGCATGTTG+GGG + chr4_4:81966881-81966900 Msa0708410:CDS 45.0%
! GGGATCGATGTTATTTGGTC+TGG + chr4_4:81966901-81966920 Msa0708410:CDS 45.0%
!! TCTTTGGAGAGTCAGGTTGG+AGG + chr4_4:81963191-81963210 Msa0708410:five_prime_UTR 50.0%
GGCAACTAAAGAAGACCTGG+AGG + chr4_4:81963647-81963666 Msa0708410:five_prime_UTR 50.0%
GGAAACTATTGAAGGCCTCC+AGG - chr4_4:81963665-81963684 None:intergenic 50.0%
! TCTTTTCTGCCAAGCAACGC+TGG - chr4_4:81964767-81964786 None:intergenic 50.0%
CCCTGGTCATGATCGGAATT+TGG - chr4_4:81964950-81964969 None:intergenic 50.0%
AATTCCGATCATGACCAGGG+GGG + chr4_4:81964950-81964969 Msa0708410:intron 50.0%
TACACCAAGTGCAGGCAAGT+TGG + chr4_4:81964987-81965006 Msa0708410:CDS 50.0%
TGCTAACTCATCGCACGCAT+GGG - chr4_4:81965267-81965286 None:intergenic 50.0%
TTGCTAACTCATCGCACGCA+TGG - chr4_4:81965268-81965287 None:intergenic 50.0%
TGGCATGGTTAACCTGCAAC+TGG - chr4_4:81965508-81965527 None:intergenic 50.0%
GCAGGTTAACCATGCCAGAA+TGG + chr4_4:81965511-81965530 Msa0708410:intron 50.0%
GGTTAACCATGCCAGAATGG+TGG + chr4_4:81965514-81965533 Msa0708410:intron 50.0%
GTTGCAACTCTCGACACGTA+TGG - chr4_4:81966451-81966470 None:intergenic 50.0%
CACCAGTTGCCATCTGAACA+TGG - chr4_4:81966810-81966829 None:intergenic 50.0%
CCACTGGTGTGCAACATTCT+AGG - chr4_4:81966954-81966973 None:intergenic 50.0%
CCTAGAATGTTGCACACCAG+TGG + chr4_4:81966951-81966970 Msa0708410:intron 50.0%
! AGAGCTGTCGATTGCCACCA+GGG - chr4_4:81963617-81963636 None:intergenic 55.0%
! AAGAGCTGTCGATTGCCACC+AGG - chr4_4:81963618-81963637 None:intergenic 55.0%
GGTGGCAATCGACAGCTCTT+CGG + chr4_4:81963618-81963637 Msa0708410:intron 55.0%
TCGACAGCTCTTCGGATGCA+TGG + chr4_4:81963626-81963645 Msa0708410:intron 55.0%
GGCCATGTCAAGGTTCTCAG+AGG - chr4_4:81963719-81963738 None:intergenic 55.0%
ATCTCCAGAGCAGCATGCAG+AGG + chr4_4:81963904-81963923 Msa0708410:five_prime_UTR 55.0%
GAAACCTCTGCATGCTGCTC+TGG - chr4_4:81963911-81963930 None:intergenic 55.0%
GTCATTTGGCTGCAGCAAGC+AGG + chr4_4:81964232-81964251 Msa0708410:five_prime_UTR 55.0%
TCCTATTCTGCAAGGCAGCG+AGG + chr4_4:81964327-81964346 Msa0708410:intron 55.0%
TCCTCGCTGCCTTGCAGAAT+AGG - chr4_4:81964331-81964350 None:intergenic 55.0%
GCAAGGCAGCGAGGACATAA+TGG + chr4_4:81964336-81964355 Msa0708410:intron 55.0%
ATGGTAAGCGGTTCACTCCC+AGG + chr4_4:81964716-81964735 Msa0708410:CDS 55.0%
AAGGATGCACCAGCGTTGCT+TGG + chr4_4:81964755-81964774 Msa0708410:CDS 55.0%
! GCATCTAAAACATGCCCCCC+TGG - chr4_4:81964967-81964986 None:intergenic 55.0%
TCTTCTGCATTGCTCGGCTG+TGG + chr4_4:81966777-81966796 Msa0708410:intron 55.0%
! CCAGCTCCGATTTGACGACA+AGG - chr4_4:81966841-81966860 None:intergenic 55.0%
CCTTGTCGTCAAATCGGAGC+TGG + chr4_4:81966838-81966857 Msa0708410:CDS 55.0%
CATGCCCCCCTGGTCATGAT+CGG - chr4_4:81964957-81964976 None:intergenic 60.0%
CCAGCCAACTTGCCTGCACT+TGG - chr4_4:81964994-81965013 None:intergenic 60.0%
! CCAAGTGCAGGCAAGTTGGC+TGG + chr4_4:81964991-81965010 Msa0708410:CDS 60.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 81963077 81967091 81963077 ID=Msa0708410;Name=Msa0708410
chr4_4 mRNA 81963077 81967091 81963077 ID=Msa0708410-mRNA-1;Parent=Msa0708410;Name=Msa0708410-mRNA-1;_AED=0.20;_eAED=0.20;_QI=1082|0.62|0.55|1|0.62|0.55|9|0|203
chr4_4 exon 81963077 81963352 81963077 ID=Msa0708410-mRNA-1:exon:26927;Parent=Msa0708410-mRNA-1
chr4_4 exon 81963633 81964253 81963633 ID=Msa0708410-mRNA-1:exon:26928;Parent=Msa0708410-mRNA-1
chr4_4 exon 81964445 81964594 81964445 ID=Msa0708410-mRNA-1:exon:26929;Parent=Msa0708410-mRNA-1
chr4_4 exon 81964681 81964810 81964681 ID=Msa0708410-mRNA-1:exon:26930;Parent=Msa0708410-mRNA-1
chr4_4 exon 81964968 81965038 81964968 ID=Msa0708410-mRNA-1:exon:26931;Parent=Msa0708410-mRNA-1
chr4_4 exon 81965588 81965832 81965588 ID=Msa0708410-mRNA-1:exon:26932;Parent=Msa0708410-mRNA-1
chr4_4 exon 81966656 81966700 81966656 ID=Msa0708410-mRNA-1:exon:26933;Parent=Msa0708410-mRNA-1
chr4_4 exon 81966817 81966922 81966817 ID=Msa0708410-mRNA-1:exon:26934;Parent=Msa0708410-mRNA-1
chr4_4 exon 81967042 81967091 81967042 ID=Msa0708410-mRNA-1:exon:26935;Parent=Msa0708410-mRNA-1
chr4_4 five_prime_UTR 81963077 81963352 81963077 ID=Msa0708410-mRNA-1:five_prime_utr;Parent=Msa0708410-mRNA-1
chr4_4 five_prime_UTR 81963633 81964253 81963633 ID=Msa0708410-mRNA-1:five_prime_utr;Parent=Msa0708410-mRNA-1
chr4_4 five_prime_UTR 81964445 81964594 81964445 ID=Msa0708410-mRNA-1:five_prime_utr;Parent=Msa0708410-mRNA-1
chr4_4 five_prime_UTR 81964681 81964715 81964681 ID=Msa0708410-mRNA-1:five_prime_utr;Parent=Msa0708410-mRNA-1
chr4_4 CDS 81964716 81964810 81964716 ID=Msa0708410-mRNA-1:cds;Parent=Msa0708410-mRNA-1
chr4_4 CDS 81964968 81965038 81964968 ID=Msa0708410-mRNA-1:cds;Parent=Msa0708410-mRNA-1
chr4_4 CDS 81965588 81965832 81965588 ID=Msa0708410-mRNA-1:cds;Parent=Msa0708410-mRNA-1
chr4_4 CDS 81966656 81966700 81966656 ID=Msa0708410-mRNA-1:cds;Parent=Msa0708410-mRNA-1
chr4_4 CDS 81966817 81966922 81966817 ID=Msa0708410-mRNA-1:cds;Parent=Msa0708410-mRNA-1
chr4_4 CDS 81967042 81967091 81967042 ID=Msa0708410-mRNA-1:cds;Parent=Msa0708410-mRNA-1
Gene Sequence

>Msa0708410

ATGGTAAGCGGTTCACTCCCAGGTTCTAGCAAAGTAACGAAGGATGCACCAGCGTTGCTTGGCAGAAAAGATGATCTTAAAAGTTGTAATACCAGGGGGGCATGTTTTAGATGCTACACCAAGTGCAGGCAAGTTGGCTGGAAATATGAAATCATTTCACGAAGATGTAAAACAAGGCTCAAGTATGAAATGAGGTTCTACCAAGAGATGGTTGTGCATCAAATTCGACAAATGAGTGAGCACAACCAGCAACTTCTTTATTTGAAGAACAAGATGGTCAAAGAGAAGAAACATTCAAAATCTCTTGAGGAATCCTTTGGTATAGTGACCGAGAAACTGCGTAAAACCATGGAGGAGAATCACATTGTGAGGCGGAGAACAAAAATGCAGCATGAAGAAATAAAAGAAGAGGAGACAATTTCAACTGGTTTATTCCTTCTGCAAAATAGAGCAGTGATGGCAACTGGTGCATTTCAGCCTTGTCGTCAAATCGGAGCTGGAATTCATTGCATCTGTGTAGATGTAGTTGGAGGCATGTTGGGGATCGATGTTATTTGGTCTGGATACATAGTGAGGCTAATAGTTTTAAGGCTATATCATGGGAGTCATTAA
Protein sequence

>Msa0708410

MVSGSLPGSSKVTKDAPALLGRKDDLKSCNTRGACFRCYTKCRQVGWKYEIISRRCKTRLKYEMRFYQEMVVHQIRQMSEHNQQLLYLKNKMVKEKKHSKSLEESFGIVTEKLRKTMEENHIVRRRTKMQHEEIKEEETISTGLFLLQNRAVMATGAFQPCRQIGAGIHCICVDVVGGMLGIDVIWSGYIVRLIVLRLYHGSH*