AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0363540

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 62 sgRNAs with CRISPR-Local

Find 196 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTCCTTTATGCATATTCT+TGG 0.250199 3_1:+56123382 None:intergenic
GTCTTTGTCTTCATGCGTTT+TGG 0.261305 3_1:-56124511 Msa0363540:three_prime_UTR
ATTGAATTGAAGAAGGAAAT+TGG 0.266730 3_1:+56126275 None:intergenic
AGACAAGAAAGTACACTTTA+TGG 0.268266 3_1:-56123338 Msa0363540:three_prime_UTR
CAGAATAGGTTGTTGTAGTT+TGG 0.288548 3_1:-56124423 Msa0363540:three_prime_UTR
ACACCACCATCCATAAAATA+AGG 0.290432 3_1:+56125352 None:intergenic
TCTTCAATTCAATTCATTGA+TGG 0.291561 3_1:-56126265 Msa0363540:CDS
CATTACTAATGAAAGAAGTA+TGG 0.295388 3_1:+56123160 None:intergenic
CAACATGCCTTGAAAATTAT+AGG 0.302533 3_1:-56123413 Msa0363540:three_prime_UTR
AAGAATATGCATAAAGGAAT+TGG 0.305271 3_1:-56123380 Msa0363540:three_prime_UTR
AAGAGTGATGAGAGTAGAAT+TGG 0.309870 3_1:+56126303 None:intergenic
TATGACAAAGGTTTGTAGTA+TGG 0.322671 3_1:-56123244 Msa0363540:three_prime_UTR
ACCTTCGTTGTTCAGGTTTG+TGG 0.332260 3_1:-56126361 Msa0363540:intron
GATTGTTTCTTATGTTCTCT+TGG 0.354582 3_1:-56124363 Msa0363540:three_prime_UTR
CAAACTACAACAACCTATTC+TGG 0.370843 3_1:+56124424 None:intergenic
GATGATCGTTAAAACAACTT+AGG 0.378362 3_1:-56123287 Msa0363540:three_prime_UTR
ATGATCGTTAAAACAACTTA+GGG 0.382171 3_1:-56123286 Msa0363540:three_prime_UTR
GTTGTAATGAGTATTGTAAT+TGG 0.394623 3_1:-56123208 Msa0363540:three_prime_UTR
CACCACCATCCATAAAATAA+GGG 0.410936 3_1:+56125353 None:intergenic
CACTTTATGGCATATGTTGA+TGG 0.413051 3_1:-56123325 Msa0363540:three_prime_UTR
CGCGTCAAGAAAGCAAAATT+AGG 0.432541 3_1:-56124184 Msa0363540:three_prime_UTR
TGGCCATTGAAGAACTGCTT+GGG 0.436157 3_1:-56124151 Msa0363540:three_prime_UTR
ATTTCGTGAAGTTTCTTTCA+AGG 0.443398 3_1:-56126338 Msa0363540:CDS
GTTGTGCAGCAGCAATGTTT+TGG 0.448999 3_1:-56124632 Msa0363540:CDS
CCCGTCCAGATCTTCACTTC+CGG 0.451255 3_1:-56126578 Msa0363540:CDS
AACTATTTAGAGCTGGTTGA+AGG 0.455439 3_1:-56124734 Msa0363540:CDS
ACAACCAAGAATATGCATAA+AGG 0.458992 3_1:-56123386 Msa0363540:three_prime_UTR
TCCGAGTGGTGTACCGGAGG+TGG 0.463387 3_1:+56126555 None:intergenic
TAAAACCGATACGGTGCAGC+TGG 0.468450 3_1:+56124547 None:intergenic
TCACAACAATTAACCCACTA+AGG 0.471654 3_1:+56124211 None:intergenic
TGATGAACAACGACGGCCTC+AGG 0.472080 3_1:+56126613 None:intergenic
GGTGGCCGGAAGTGAAGATC+TGG 0.472543 3_1:+56126573 None:intergenic
GTGTTCCAGCTGCACCGTAT+CGG 0.487143 3_1:-56124552 Msa0363540:three_prime_UTR
TTATGGATGGTGGTGTGGCT+AGG 0.508220 3_1:-56125345 Msa0363540:CDS
GTTGATATTTACTATGACAA+AGG 0.508489 3_1:-56123256 Msa0363540:three_prime_UTR
TGTGCTTAACTATTTAGAGC+TGG 0.508616 3_1:-56124741 Msa0363540:CDS
GCGTGTATTTATATACGTTC+CGG 0.510438 3_1:-56123449 Msa0363540:three_prime_UTR
ATGGCATATGTTGATGGAAG+TGG 0.511028 3_1:-56123319 Msa0363540:three_prime_UTR
AGCCCCAAGCAGTTCTTCAA+TGG 0.511679 3_1:+56124148 None:intergenic
ATTACTAATGAAAGAAGTAT+GGG 0.514730 3_1:+56123161 None:intergenic
TCTTCACTTCCGGCCACCTC+CGG 0.515234 3_1:-56126568 Msa0363540:intron
CAAAATTAGGACATAGTACA+TGG 0.523094 3_1:-56124171 Msa0363540:three_prime_UTR
AGTGGTGTACCGGAGGTGGC+CGG 0.533456 3_1:+56126559 None:intergenic
TCAATGAATTGAATTGAAGA+AGG 0.538508 3_1:+56126268 None:intergenic
ATGGCCATTGAAGAACTGCT+TGG 0.540836 3_1:-56124152 Msa0363540:three_prime_UTR
AGAGTGATGAGAGTAGAATT+GGG 0.545147 3_1:+56126304 None:intergenic
TATTTATATACGTTCCGGAC+TGG 0.545815 3_1:-56123444 Msa0363540:three_prime_UTR
TAGTTATGAAGTAGCAGTAA+TGG 0.550137 3_1:+56124321 None:intergenic
AGTATTGTAATTGGTAGTGT+TGG 0.553310 3_1:-56123199 Msa0363540:three_prime_UTR
ATGTTGGCGCCACACCAGTC+CGG 0.563134 3_1:+56123430 None:intergenic
CCGGAAGTGAAGATCTGGAC+GGG 0.569730 3_1:+56126578 None:intergenic
AGAAGAAAATAAAACCGATA+CGG 0.574139 3_1:+56124538 None:intergenic
CATAATTTGGAGCGAGATCG+AGG 0.579379 3_1:-56124701 Msa0363540:CDS
ATATACGTTCCGGACTGGTG+TGG 0.595552 3_1:-56123439 Msa0363540:three_prime_UTR
ACCACCATCCATAAAATAAG+GGG 0.622942 3_1:+56125354 None:intergenic
GGCCATTGAAGAACTGCTTG+GGG 0.625082 3_1:-56124150 Msa0363540:three_prime_UTR
GTATTGCAGCTGCTACGTAG+TGG 0.628929 3_1:-56124610 Msa0363540:exon
GCCGGAAGTGAAGATCTGGA+CGG 0.644602 3_1:+56126577 None:intergenic
CTATGCTCTTCATCCTCCTG+AGG 0.644897 3_1:-56126629 Msa0363540:CDS
TGAACAACGACGGCCTCAGG+AGG 0.651286 3_1:+56126616 None:intergenic
TGATCGTTAAAACAACTTAG+GGG 0.690002 3_1:-56123285 Msa0363540:three_prime_UTR
TTACTAATGAAAGAAGTATG+GGG 0.695748 3_1:+56123162 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTTTTAAATTATATTTA+TGG - chr3_1:56124206-56124225 Msa0363540:three_prime_UTR 0.0%
!!! TTTTTAAATTATATTTATGG+TGG - chr3_1:56124209-56124228 Msa0363540:three_prime_UTR 10.0%
!!! AGAATTATATCATATATTTT+AGG - chr3_1:56124712-56124731 Msa0363540:CDS 10.0%
!!! AATTTTTTTCTTTGTTATAA+AGG - chr3_1:56125774-56125793 Msa0363540:intron 10.0%
!!! AGTGACAAATTTTTATTTTA+TGG - chr3_1:56123846-56123865 Msa0363540:intron 15.0%
!!! ATAATTTAGAGTTGTTATTT+TGG - chr3_1:56123998-56124017 Msa0363540:intron 15.0%
!!! AAAATTATTGTCTTTTTAAC+TGG - chr3_1:56125290-56125309 Msa0363540:intron 15.0%
!!! GTTTTTAACTTATTTTTGTA+AGG + chr3_1:56125536-56125555 None:intergenic 15.0%
!! TGTAAGTAAAAAGTAAATAT+TGG - chr3_1:56126108-56126127 Msa0363540:intron 15.0%
!! GTTGAAACAAATTACAAAAA+AGG + chr3_1:56123598-56123617 None:intergenic 20.0%
!!! TTGTAATTTGTTTCAACTAA+TGG - chr3_1:56123601-56123620 Msa0363540:intron 20.0%
!! ATTGAAGACATAATTAAGAA+TGG + chr3_1:56123757-56123776 None:intergenic 20.0%
!! TTCTTAATTATGTCTTCAAT+TGG - chr3_1:56123757-56123776 Msa0363540:intron 20.0%
!!! CAATCTAATAGACATTATTT+TGG - chr3_1:56123906-56123925 Msa0363540:intron 20.0%
!!! AGATGAGTTTATATTTTTCT+TGG - chr3_1:56124092-56124111 Msa0363540:intron 20.0%
!!! GATGAGTTTATATTTTTCTT+GGG - chr3_1:56124093-56124112 Msa0363540:intron 20.0%
!!! TATTGAATTATCTTGCTTTT+TGG - chr3_1:56124239-56124258 Msa0363540:three_prime_UTR 20.0%
!!! AAAATCAAGTTTTGTTTGAT+GGG - chr3_1:56124333-56124352 Msa0363540:three_prime_UTR 20.0%
!!! TATTTTTTCCCCTTATTTTA+TGG - chr3_1:56124404-56124423 Msa0363540:three_prime_UTR 20.0%
!! TAACAACAAAAATAAATACG+TGG + chr3_1:56124524-56124543 None:intergenic 20.0%
!! GAAACTTTACATAAAATTTG+CGG + chr3_1:56124772-56124791 None:intergenic 20.0%
!! ATGCATAATTCAACAAAAAT+AGG + chr3_1:56124936-56124955 None:intergenic 20.0%
!! AAATAAGTTAAAAACCTTAG+TGG - chr3_1:56125541-56125560 Msa0363540:intron 20.0%
!! AATAAGTTAAAAACCTTAGT+GGG - chr3_1:56125542-56125561 Msa0363540:intron 20.0%
!!! TTAATTTGTGAGTATAAGTT+TGG - chr3_1:56125654-56125673 Msa0363540:intron 20.0%
!!! ATTTAAAATAACTACCAACA+GGG + chr3_1:56125942-56125961 None:intergenic 20.0%
!!! GTAGTTATTTTAAATGTTGT+AGG - chr3_1:56125947-56125966 Msa0363540:intron 20.0%
!! TTAAAGTCTTTGTAAAATCA+AGG - chr3_1:56126014-56126033 Msa0363540:intron 20.0%
!!! TTGGAATTTTGAGTATATTT+TGG - chr3_1:56126127-56126146 Msa0363540:intron 20.0%
!!! ATTACTAATGAAAGAAGTAT+GGG + chr3_1:56126608-56126627 None:intergenic 20.0%
!!! ATTGCTTTTTTGTTGATGAA+GGG + chr3_1:56123308-56123327 None:intergenic 25.0%
!!! AAGCTTTTTTTGATGAAGAA+GGG + chr3_1:56123346-56123365 None:intergenic 25.0%
!!! AAAGCTTTTTTTGATGAAGA+AGG + chr3_1:56123347-56123366 None:intergenic 25.0%
! ATTGAATTGAAGAAGGAAAT+TGG + chr3_1:56123494-56123513 None:intergenic 25.0%
! TCAATGAATTGAATTGAAGA+AGG + chr3_1:56123501-56123520 None:intergenic 25.0%
! TCTTCAATTCAATTCATTGA+TGG - chr3_1:56123501-56123520 Msa0363540:intron 25.0%
!!! ATAACCTAAGCTATTTTTCA+GGG + chr3_1:56123543-56123562 None:intergenic 25.0%
!!! AATAACCTAAGCTATTTTTC+AGG + chr3_1:56123544-56123563 None:intergenic 25.0%
!! TCAGAATTCTTTTCTTACAA+AGG - chr3_1:56123643-56123662 Msa0363540:intron 25.0%
!! CAGAATTCTTTTCTTACAAA+GGG - chr3_1:56123644-56123663 Msa0363540:intron 25.0%
! ATTTATTCTACTATTTGCTC+TGG - chr3_1:56124157-56124176 Msa0363540:three_prime_UTR 25.0%
!!! CAAAATCAAGTTTTGTTTGA+TGG - chr3_1:56124332-56124351 Msa0363540:three_prime_UTR 25.0%
! ATAAGGGGAAAAAATAACAT+AGG + chr3_1:56124400-56124419 None:intergenic 25.0%
! TTTGATGAGAAGATGAAATT+TGG - chr3_1:56124481-56124500 Msa0363540:three_prime_UTR 25.0%
! AACAAAAATAAATACGTGGA+GGG + chr3_1:56124520-56124539 None:intergenic 25.0%
!!! TTTTGTTGTGAGTTTATGTT+AGG - chr3_1:56124573-56124592 Msa0363540:three_prime_UTR 25.0%
!! ATTTTGTCTCATTCTGTTTT+TGG - chr3_1:56124602-56124621 Msa0363540:exon 25.0%
! TGAAAGAAAAAAGATACAGT+TGG - chr3_1:56124678-56124697 Msa0363540:CDS 25.0%
!! CATTCCATAAGCATATATTT+TGG + chr3_1:56124979-56124998 None:intergenic 25.0%
!! AGGTAATTTTCTGCATAATT+TGG - chr3_1:56125052-56125071 Msa0363540:intron 25.0%
! AGAAGAAAATAAAACCGATA+CGG + chr3_1:56125231-56125250 None:intergenic 25.0%
!!! TGATTTTGCTTTGTTTTCTT+TGG - chr3_1:56125380-56125399 Msa0363540:CDS 25.0%
!!! GATTTTGCTTTGTTTTCTTT+GGG - chr3_1:56125381-56125400 Msa0363540:intron 25.0%
!! TTTTATGTTGTGTCAACTAT+AGG - chr3_1:56125506-56125525 Msa0363540:intron 25.0%
! TTATCAAAGATCATATGCTA+TGG - chr3_1:56125817-56125836 Msa0363540:intron 25.0%
!! CTTGTAGTCTTAATTTGATA+TGG + chr3_1:56125880-56125899 None:intergenic 25.0%
!! TTTAAAATAACTACCAACAG+GGG + chr3_1:56125941-56125960 None:intergenic 25.0%
!! CATTTAAAATAACTACCAAC+AGG + chr3_1:56125943-56125962 None:intergenic 25.0%
! ACCTTACAAATTGATCAAAA+TGG + chr3_1:56126153-56126172 None:intergenic 25.0%
!!! CTTGGTTTTATGATTTTTCA+AGG - chr3_1:56126203-56126222 Msa0363540:intron 25.0%
!! TGTGATACCTATAATTTTCA+AGG + chr3_1:56126363-56126382 None:intergenic 25.0%
! AATTCCTTTATGCATATTCT+TGG + chr3_1:56126387-56126406 None:intergenic 25.0%
! AAGAATATGCATAAAGGAAT+TGG - chr3_1:56126386-56126405 Msa0363540:intron 25.0%
! ATGATCGTTAAAACAACTTA+GGG - chr3_1:56126480-56126499 Msa0363540:intron 25.0%
! GTTGATATTTACTATGACAA+AGG - chr3_1:56126510-56126529 Msa0363540:intron 25.0%
!! GTTGTAATGAGTATTGTAAT+TGG - chr3_1:56126558-56126577 Msa0363540:intron 25.0%
!! TTACTAATGAAAGAAGTATG+GGG + chr3_1:56126607-56126626 None:intergenic 25.0%
! CATTACTAATGAAAGAAGTA+TGG + chr3_1:56126609-56126628 None:intergenic 25.0%
!! TTTTTGTTGATGAACAACGA+CGG + chr3_1:56123163-56123182 None:intergenic 30.0%
! GATTTGGATTTTATGTGAGA+TGG + chr3_1:56123246-56123265 None:intergenic 30.0%
!!! TTGCTTTTTTGTTGATGAAG+GGG + chr3_1:56123307-56123326 None:intergenic 30.0%
!!! GATTGCTTTTTTGTTGATGA+AGG + chr3_1:56123309-56123328 None:intergenic 30.0%
ATTTCGTGAAGTTTCTTTCA+AGG - chr3_1:56123428-56123447 Msa0363540:three_prime_UTR 30.0%
CTTAGGTTATTACCACTAAT+TGG - chr3_1:56123553-56123572 Msa0363540:intron 30.0%
AACAAAAAGTAGCCAATTAG+TGG + chr3_1:56123568-56123587 None:intergenic 30.0%
AAACAACCATTGTTTACCTT+GGG + chr3_1:56123732-56123751 None:intergenic 30.0%
TAAACAACCATTGTTTACCT+TGG + chr3_1:56123733-56123752 None:intergenic 30.0%
GTTGTACAACAACATTTCAT+AGG + chr3_1:56124054-56124073 None:intergenic 30.0%
AAATTTAACACCACCAACTT+AGG - chr3_1:56124294-56124313 Msa0363540:three_prime_UTR 30.0%
!!! TTTTCCCCTTATTTTATGGA+TGG - chr3_1:56124408-56124427 Msa0363540:three_prime_UTR 30.0%
CAACAAAAATAAATACGTGG+AGG + chr3_1:56124521-56124540 None:intergenic 30.0%
TACATAAAATTTGCGGACAT+CGG + chr3_1:56124765-56124784 None:intergenic 30.0%
! ACATAATGATTAAAGAGCAC+AGG + chr3_1:56124799-56124818 None:intergenic 30.0%
AGCTCCAAAATATATGCTTA+TGG - chr3_1:56124972-56124991 Msa0363540:intron 30.0%
TAAATAGTTAAGCACACATC+TGG + chr3_1:56125021-56125040 None:intergenic 30.0%
!! TTTTCTGCATAATTTAGAGC+TGG - chr3_1:56125091-56125110 Msa0363540:intron 30.0%
!!! CATAATTTAGAGCTGGTTTT+CGG - chr3_1:56125098-56125117 Msa0363540:intron 30.0%
! GATTGTTTCTTATGTTCTCT+TGG - chr3_1:56125403-56125422 Msa0363540:intron 30.0%
!!! TCTCTTGGTTCTTATTTTCT+TGG - chr3_1:56125418-56125437 Msa0363540:intron 30.0%
!!! CTCTTGGTTCTTATTTTCTT+GGG - chr3_1:56125419-56125438 Msa0363540:intron 30.0%
TAGTTATGAAGTAGCAGTAA+TGG + chr3_1:56125448-56125467 None:intergenic 30.0%
CAAAATTAGGACATAGTACA+TGG - chr3_1:56125595-56125614 Msa0363540:intron 30.0%
!! GCCATTTTGATCAATTTGTA+AGG - chr3_1:56126149-56126168 Msa0363540:intron 30.0%
AAGAAAGAACTTTCAAACAG+TGG + chr3_1:56126181-56126200 None:intergenic 30.0%
TTGAAAGTTCTTTCTTTGCT+TGG - chr3_1:56126185-56126204 Msa0363540:intron 30.0%
! TTCTTTTGTTGCACATGATT+TGG - chr3_1:56126286-56126305 Msa0363540:CDS 30.0%
!! CTATAATTTTCAAGGCATGT+TGG + chr3_1:56126355-56126374 None:intergenic 30.0%
CAACATGCCTTGAAAATTAT+AGG - chr3_1:56126353-56126372 Msa0363540:intron 30.0%
ACAACCAAGAATATGCATAA+AGG - chr3_1:56126380-56126399 Msa0363540:intron 30.0%
! AGACAAGAAAGTACACTTTA+TGG - chr3_1:56126428-56126447 Msa0363540:intron 30.0%
GATGATCGTTAAAACAACTT+AGG - chr3_1:56126479-56126498 Msa0363540:intron 30.0%
TGATCGTTAAAACAACTTAG+GGG - chr3_1:56126481-56126500 Msa0363540:intron 30.0%
TATGACAAAGGTTTGTAGTA+TGG - chr3_1:56126522-56126541 Msa0363540:intron 30.0%
!! AGTATTGTAATTGGTAGTGT+TGG - chr3_1:56126567-56126586 Msa0363540:intron 30.0%
!!! TGCTTTTTTGTTGATGAAGG+GGG + chr3_1:56123306-56123325 None:intergenic 35.0%
AGAGTGATGAGAGTAGAATT+GGG + chr3_1:56123465-56123484 None:intergenic 35.0%
AAGAGTGATGAGAGTAGAAT+TGG + chr3_1:56123466-56123485 None:intergenic 35.0%
! ATTGATGGCTGTTAAGTGAA+AGG - chr3_1:56123516-56123535 Msa0363540:intron 35.0%
! TTGATGGCTGTTAAGTGAAA+GGG - chr3_1:56123517-56123536 Msa0363540:intron 35.0%
! CTTTTCTTACAAAGGGATGA+TGG - chr3_1:56123651-56123670 Msa0363540:intron 35.0%
TCGATGACTGCATTATAGAA+TGG - chr3_1:56123701-56123720 Msa0363540:intron 35.0%
GACTGCATTATAGAATGGAT+AGG - chr3_1:56123706-56123725 Msa0363540:intron 35.0%
!!! AGACATTATTTTGGCTGAGT+AGG - chr3_1:56123915-56123934 Msa0363540:intron 35.0%
! GAAATCTATTGTGTTTTCCC+AGG - chr3_1:56124020-56124039 Msa0363540:intron 35.0%
! TTGTGTTTTCCCAGGTATTA+TGG - chr3_1:56124028-56124047 Msa0363540:intron 35.0%
TTTCATAGGCCATAATACCT+GGG + chr3_1:56124040-56124059 None:intergenic 35.0%
ATTTCATAGGCCATAATACC+TGG + chr3_1:56124041-56124060 None:intergenic 35.0%
AATCTTACTTGATGTCACTG+TGG - chr3_1:56124360-56124379 Msa0363540:three_prime_UTR 35.0%
ACCACCATCCATAAAATAAG+GGG + chr3_1:56124415-56124434 None:intergenic 35.0%
CACCACCATCCATAAAATAA+GGG + chr3_1:56124416-56124435 None:intergenic 35.0%
ACACCACCATCCATAAAATA+AGG + chr3_1:56124417-56124436 None:intergenic 35.0%
!! TTATTTTATGGATGGTGGTG+TGG - chr3_1:56124416-56124435 Msa0363540:three_prime_UTR 35.0%
TGAGAAGATGAAATTTGGAC+AGG - chr3_1:56124486-56124505 Msa0363540:three_prime_UTR 35.0%
!!! TGTCTCATTCTGTTTTTGGT+AGG - chr3_1:56124606-56124625 Msa0363540:exon 35.0%
! CAATCAGAAGCATGAAATAG+AGG + chr3_1:56124905-56124924 None:intergenic 35.0%
!!! TTTTGGAGCTATGCTAACTA+AGG + chr3_1:56124962-56124981 None:intergenic 35.0%
! TGTGCTTAACTATTTAGAGC+TGG - chr3_1:56125025-56125044 Msa0363540:intron 35.0%
! AACTATTTAGAGCTGGTTGA+AGG - chr3_1:56125032-56125051 Msa0363540:intron 35.0%
CAAACTACAACAACCTATTC+TGG + chr3_1:56125345-56125364 None:intergenic 35.0%
CAGAATAGGTTGTTGTAGTT+TGG - chr3_1:56125343-56125362 Msa0363540:CDS 35.0%
TCACAACAATTAACCCACTA+AGG + chr3_1:56125558-56125577 None:intergenic 35.0%
CAGTGAAAGTCAACTGATAA+AGG - chr3_1:56125702-56125721 Msa0363540:intron 35.0%
! TGGATTTTGAACAACTCAGT+TGG - chr3_1:56125837-56125856 Msa0363540:intron 35.0%
GCGTGTATTTATATACGTTC+CGG - chr3_1:56126317-56126336 Msa0363540:CDS 35.0%
TATTTATATACGTTCCGGAC+TGG - chr3_1:56126322-56126341 Msa0363540:CDS 35.0%
CACTTTATGGCATATGTTGA+TGG - chr3_1:56126441-56126460 Msa0363540:intron 35.0%
! TGGATTTTATGTGAGATGGC+CGG + chr3_1:56123242-56123261 None:intergenic 40.0%
GATGTTCGAGATTCAGCAAA+AGG + chr3_1:56123372-56123391 None:intergenic 40.0%
GTTATCTACCTTCGTTGTTC+AGG - chr3_1:56123398-56123417 Msa0363540:three_prime_UTR 40.0%
!! TCTTACAAAGGGATGATGGA+AGG - chr3_1:56123655-56123674 Msa0363540:intron 40.0%
TTATAGAATGGATAGGCCCA+AGG - chr3_1:56123713-56123732 Msa0363540:intron 40.0%
! ATATCGAAGTGTTAGCGTGA+CGG - chr3_1:56123947-56123966 Msa0363540:intron 40.0%
!!! TCCCCTTATTTTATGGATGG+TGG - chr3_1:56124411-56124430 Msa0363540:three_prime_UTR 40.0%
AGAAAAATCAGAACACAGCG+AGG + chr3_1:56125190-56125209 None:intergenic 40.0%
! GTCTTTGTCTTCATGCGTTT+TGG - chr3_1:56125255-56125274 Msa0363540:intron 40.0%
! CCTATTCTGGAAAAAGCAAC+AGG + chr3_1:56125332-56125351 None:intergenic 40.0%
!!! CCTGTTGCTTTTTCCAGAAT+AGG - chr3_1:56125329-56125348 Msa0363540:CDS 40.0%
CGCGTCAAGAAAGCAAAATT+AGG - chr3_1:56125582-56125601 Msa0363540:intron 40.0%
TGAAATAAATGTGCCCCTGT+TGG - chr3_1:56125925-56125944 Msa0363540:intron 40.0%
! ATGGCATATGTTGATGGAAG+TGG - chr3_1:56126447-56126466 Msa0363540:intron 40.0%
GAGAGAGAGAGAGAGAGATT+TGG + chr3_1:56123262-56123281 None:intergenic 45.0%
AAGAAGGGAGAGAACAACGA+CGG + chr3_1:56123331-56123350 None:intergenic 45.0%
! ACCTTCGTTGTTCAGGTTTG+TGG - chr3_1:56123405-56123424 Msa0363540:three_prime_UTR 45.0%
TCCACAAACCTGAACAACGA+AGG + chr3_1:56123409-56123428 None:intergenic 45.0%
AGGGCCCTGAAAAATAGCTT+AGG - chr3_1:56123536-56123555 Msa0363540:intron 45.0%
GATAGGCCCAAGGTAAACAA+TGG - chr3_1:56123723-56123742 Msa0363540:intron 45.0%
TAAGCTGCAACTGGATCAAG+TGG + chr3_1:56124132-56124151 None:intergenic 45.0%
TTGATCCAGTTGCAGCTTAG+CGG - chr3_1:56124133-56124152 Msa0363540:three_prime_UTR 45.0%
ACTCCTGCTTCATCCTAAGT+TGG + chr3_1:56124310-56124329 None:intergenic 45.0%
! CATAATTTGGAGCGAGATCG+AGG - chr3_1:56125065-56125084 Msa0363540:intron 45.0%
! GTTGTGCAGCAGCAATGTTT+TGG - chr3_1:56125134-56125153 Msa0363540:intron 45.0%
ATGGCCATTGAAGAACTGCT+TGG - chr3_1:56125614-56125633 Msa0363540:intron 45.0%
TGGCCATTGAAGAACTGCTT+GGG - chr3_1:56125615-56125634 Msa0363540:intron 45.0%
GATATGGAATGAGCCATGCT+TGG + chr3_1:56125864-56125883 None:intergenic 45.0%
! TGCACATGATTTGGCAGATG+TGG - chr3_1:56126295-56126314 Msa0363540:CDS 45.0%
! GAGTTTTCCGAGTGGTGTAC+CGG + chr3_1:56123220-56123239 None:intergenic 50.0%
GTACACCACTCGGAAAACTC+CGG - chr3_1:56123220-56123239 Msa0363540:three_prime_UTR 50.0%
GCAACTGGATCAAGTGGAGA+CGG + chr3_1:56124126-56124145 None:intergenic 50.0%
AAATGCCGCTAAGCTGCAAC+TGG + chr3_1:56124141-56124160 None:intergenic 50.0%
! CCTGCTTCATCCTAAGTTGG+TGG + chr3_1:56124307-56124326 None:intergenic 50.0%
CCACCAACTTAGGATGAAGC+AGG - chr3_1:56124304-56124323 Msa0363540:three_prime_UTR 50.0%
CTTGATGTCACTGTGGTTGC+AGG - chr3_1:56124367-56124386 Msa0363540:three_prime_UTR 50.0%
! TTATGGATGGTGGTGTGGCT+AGG - chr3_1:56124421-56124440 Msa0363540:three_prime_UTR 50.0%
!!! TAGAGCTGGTTTTCGGCATG+TGG - chr3_1:56125105-56125124 Msa0363540:intron 50.0%
!! GGTTTTCGGCATGTGGACTA+AGG - chr3_1:56125112-56125131 Msa0363540:intron 50.0%
GTATTGCAGCTGCTACGTAG+TGG - chr3_1:56125156-56125175 Msa0363540:intron 50.0%
TAAAACCGATACGGTGCAGC+TGG + chr3_1:56125222-56125241 None:intergenic 50.0%
GGCCATTGAAGAACTGCTTG+GGG - chr3_1:56125616-56125635 Msa0363540:intron 50.0%
AGCCCCAAGCAGTTCTTCAA+TGG + chr3_1:56125621-56125640 None:intergenic 50.0%
CAACTCAGTTGGACCAAGCA+TGG - chr3_1:56125848-56125867 Msa0363540:intron 50.0%
!! ATATACGTTCCGGACTGGTG+TGG - chr3_1:56126327-56126346 Msa0363540:CDS 50.0%
TGATGAACAACGACGGCCTC+AGG + chr3_1:56123156-56123175 None:intergenic 55.0%
CCGGAAGTGAAGATCTGGAC+GGG + chr3_1:56123191-56123210 None:intergenic 55.0%
CCCGTCCAGATCTTCACTTC+CGG - chr3_1:56123188-56123207 Msa0363540:three_prime_UTR 55.0%
GCCGGAAGTGAAGATCTGGA+CGG + chr3_1:56123192-56123211 None:intergenic 55.0%
! TTTTCCGAGTGGTGTACCGG+AGG + chr3_1:56123217-56123236 None:intergenic 55.0%
! TCGAAGTGTTAGCGTGACGG+TGG - chr3_1:56123950-56123969 Msa0363540:intron 55.0%
GTGTTCCAGCTGCACCGTAT+CGG - chr3_1:56125214-56125233 Msa0363540:intron 55.0%
TGAACAACGACGGCCTCAGG+AGG + chr3_1:56123153-56123172 None:intergenic 60.0%
GGTGGCCGGAAGTGAAGATC+TGG + chr3_1:56123196-56123215 None:intergenic 60.0%
TCTTCACTTCCGGCCACCTC+CGG - chr3_1:56123198-56123217 Msa0363540:three_prime_UTR 60.0%
! GATGGCCGGAGTTTTCCGAG+TGG + chr3_1:56123228-56123247 None:intergenic 60.0%
ATGTTGGCGCCACACCAGTC+CGG + chr3_1:56126339-56126358 None:intergenic 60.0%
AGTGGTGTACCGGAGGTGGC+CGG + chr3_1:56123210-56123229 None:intergenic 65.0%
GCCACCTCCGGTACACCACT+CGG - chr3_1:56123210-56123229 Msa0363540:three_prime_UTR 65.0%
TCCGAGTGGTGTACCGGAGG+TGG + chr3_1:56123214-56123233 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 56123139 56126649 56123139 ID=Msa0363540;Name=Msa0363540
chr3_1 mRNA 56123139 56126649 56123139 ID=Msa0363540-mRNA-1;Parent=Msa0363540;Name=Msa0363540-mRNA-1;_AED=0.31;_eAED=0.41;_QI=0|0.25|0.4|1|0.75|0.6|5|823|141
chr3_1 exon 56123139 56123476 56123139 ID=Msa0363540-mRNA-1:exon:10305;Parent=Msa0363540-mRNA-1
chr3_1 exon 56124128 56124766 56124128 ID=Msa0363540-mRNA-1:exon:10304;Parent=Msa0363540-mRNA-1
chr3_1 exon 56125315 56125399 56125315 ID=Msa0363540-mRNA-1:exon:10303;Parent=Msa0363540-mRNA-1
chr3_1 exon 56126263 56126368 56126263 ID=Msa0363540-mRNA-1:exon:10302;Parent=Msa0363540-mRNA-1
chr3_1 exon 56126569 56126649 56126569 ID=Msa0363540-mRNA-1:exon:10301;Parent=Msa0363540-mRNA-1
chr3_1 CDS 56126569 56126649 56126569 ID=Msa0363540-mRNA-1:cds;Parent=Msa0363540-mRNA-1
chr3_1 CDS 56126263 56126368 56126263 ID=Msa0363540-mRNA-1:cds;Parent=Msa0363540-mRNA-1
chr3_1 CDS 56125315 56125399 56125315 ID=Msa0363540-mRNA-1:cds;Parent=Msa0363540-mRNA-1
chr3_1 CDS 56124613 56124766 56124613 ID=Msa0363540-mRNA-1:cds;Parent=Msa0363540-mRNA-1
chr3_1 three_prime_UTR 56124128 56124612 56124128 ID=Msa0363540-mRNA-1:three_prime_utr;Parent=Msa0363540-mRNA-1
chr3_1 three_prime_UTR 56123139 56123476 56123139 ID=Msa0363540-mRNA-1:three_prime_utr;Parent=Msa0363540-mRNA-1
Gene Sequence

>Msa0363540

ATGCTCTTCATCCTCCTGAGGCCGTCGTTGTTCATCAACAAAAAACTCACCCGTCCAGATCTTCACTTCCGGCCACCTCCGGTTTGTGGATTTCGTGAAGTTTCTTTCAAGGAAATCGATTTACCCAATTCTACTCTCATCACTCTTTACATCCAATTTCCTTCTTCAATTCAATTCATTGATGGCTGTTGCAATCCTATGTTATTTTTTCCCCTTATTTTATGGATGGTGGTGTGGCTAGGTTTGAATTCATACGCTGAAGATGATGAAGAATGTGTGCTTAACTATTTAGAGCTGGTTGAAGGTAATTTTCTGCATAATTTGGAGCGAGATCGAGGTGATTTTCTGCATAATTTAGAGCTGGTTTTCGGCATGTGGACTAAGGTTGTGCAGCAGCAATGTTTTGGTATTGCAGCTGCTACGTAG
Protein sequence

>Msa0363540

MLFILLRPSLFINKKLTRPDLHFRPPPVCGFREVSFKEIDLPNSTLITLYIQFPSSIQFIDGCCNPMLFFPLILWMVVWLGLNSYAEDDEECVLNYLELVEGNFLHNLERDRGDFLHNLELVFGMWTKVVQQQCFGIAAAT*