AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0708800

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0708800 MtrunA17_Chr2g0322161 82.510 263 23 4 49 290 104 364 1.58e-151 429
Msa0708800 MtrunA17_Chr1g0170231 57.018 228 81 3 80 290 114 341 1.82e-81 249
Msa0708800 MtrunA17_Chr1g0170151 55.605 223 81 3 81 287 117 337 3.60e-67 213
Msa0708800 MtrunA17_Chr3g0137661 41.414 297 122 9 32 290 91 373 3.31e-58 191
Msa0708800 MtrunA17_Chr2g0298501 29.952 207 106 7 82 269 157 343 8.09e-26 105
Msa0708800 MtrunA17_Chr1g0209531 30.405 296 150 14 31 293 121 393 3.00e-25 104
Msa0708800 MtrunA17_Chr1g0148241 32.275 189 91 7 93 263 174 343 9.27e-25 102
Msa0708800 MtrunA17_Chr4g0071911 29.082 196 103 6 82 260 159 335 5.05e-24 100
Msa0708800 MtrunA17_Chr2g0276861 31.646 237 117 9 82 293 222 438 2.96e-23 99.0
Msa0708800 MtrunA17_Chr1g0151841 32.883 222 103 9 93 289 172 372 3.57e-23 98.2
Msa0708800 MtrunA17_Chr3g0138871 31.500 200 96 8 90 269 152 330 2.08e-22 95.9
Msa0708800 MtrunA17_Chr8g0341461 29.897 291 147 14 31 287 140 407 5.97e-22 95.1
Msa0708800 MtrunA17_Chr4g0073871 31.602 231 110 9 90 294 14 222 1.60e-21 90.5
Msa0708800 MtrunA17_Chr2g0298511 28.502 207 108 8 82 269 149 334 1.84e-21 93.2
Msa0708800 MtrunA17_Chr5g0423131 29.617 287 147 11 33 287 121 384 2.74e-21 92.8
Msa0708800 MtrunA17_Chr6g0484141 30.802 237 120 8 82 294 204 420 1.96e-20 90.9
Msa0708800 MtrunA17_Chr5g0394981 32.642 193 93 7 93 270 10 180 9.14e-20 85.9
Msa0708800 MtrunA17_Chr5g0394961 30.116 259 143 9 31 270 148 387 1.32e-19 88.2
Msa0708800 MtrunA17_Chr3g0136091 28.571 294 161 11 19 294 132 394 3.95e-18 84.0
Msa0708800 MtrunA17_Chr2g0289021 30.734 218 90 9 90 268 127 322 1.53e-17 82.0
Msa0708800 MtrunA17_Chr8g0384601 29.091 220 93 8 90 268 120 317 1.48e-13 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0708800 AT5G22070.1 65.652 230 56 4 84 290 129 358 1.12e-102 305
Msa0708800 AT3G52060.1 49.231 260 96 6 56 290 94 342 1.98e-78 242
Msa0708800 AT5G25330.1 47.257 237 86 6 80 290 135 358 7.93e-60 195
Msa0708800 AT4G32290.1 44.402 259 92 6 71 290 130 375 4.38e-53 178
Msa0708800 AT4G31350.3 29.167 264 141 10 24 264 75 315 6.76e-29 114
Msa0708800 AT4G31350.4 30.000 270 136 12 24 264 75 320 1.41e-28 113
Msa0708800 AT4G31350.1 30.000 270 136 12 24 264 75 320 1.41e-28 113
Msa0708800 AT4G31350.2 30.000 270 136 12 24 264 75 320 1.41e-28 113
Msa0708800 AT5G57270.3 33.333 186 87 6 93 260 160 326 1.39e-25 105
Msa0708800 AT5G57270.1 33.333 186 87 6 93 260 160 326 1.39e-25 105
Msa0708800 AT5G57270.4 33.333 186 87 6 93 260 160 326 1.39e-25 105
Msa0708800 AT5G57270.5 33.333 186 87 6 93 260 160 326 1.39e-25 105
Msa0708800 AT5G57270.2 33.333 186 87 6 93 260 160 326 1.39e-25 105
Msa0708800 AT4G25870.1 33.871 186 86 6 93 260 161 327 1.94e-25 104
Msa0708800 AT4G30060.2 31.034 203 105 6 82 267 160 344 4.29e-25 103
Msa0708800 AT4G30060.1 31.034 203 105 6 82 267 160 344 4.29e-25 103
Msa0708800 AT5G25970.1 32.759 232 112 10 80 287 170 381 6.11e-25 103
Msa0708800 AT5G25970.2 32.759 232 112 10 80 287 170 381 6.11e-25 103
Msa0708800 AT5G25970.3 32.759 232 112 10 80 287 170 381 6.11e-25 103
Msa0708800 AT2G19160.2 32.487 197 96 7 82 260 157 334 1.70e-24 102
Msa0708800 AT2G19160.1 32.487 197 96 7 82 260 157 334 1.70e-24 102
Msa0708800 AT1G51770.1 29.758 289 149 15 31 287 120 386 2.80e-24 102
Msa0708800 AT3G21310.1 31.197 234 121 9 82 293 168 383 1.05e-23 100
Msa0708800 AT3G21310.3 31.197 234 121 9 82 293 168 383 1.05e-23 100
Msa0708800 AT1G10280.1 30.833 240 115 11 82 293 196 412 1.28e-23 100
Msa0708800 AT5G11730.1 31.356 236 118 10 82 293 171 386 5.33e-22 95.1
Msa0708800 AT5G16170.1 31.489 235 112 10 82 287 189 403 1.86e-20 91.3
Msa0708800 AT1G68380.1 33.173 208 101 8 82 279 177 356 5.66e-20 89.7
Msa0708800 AT1G68390.1 30.000 240 122 9 80 293 189 408 1.33e-19 88.6
Msa0708800 AT1G11940.1 30.435 253 106 11 61 265 100 330 6.81e-17 80.5
Msa0708800 AT1G10880.1 29.741 232 119 10 80 287 169 380 3.63e-16 78.6
Msa0708800 AT5G14550.2 30.769 208 94 8 90 267 129 316 3.67e-16 78.2
Msa0708800 AT1G62305.1 31.604 212 88 9 93 268 137 327 4.20e-16 78.2
Msa0708800 AT5G14550.1 28.837 215 94 7 93 268 132 326 2.50e-14 73.2
Msa0708800 AT5G14550.3 28.837 215 94 7 93 268 132 326 2.51e-14 73.2
Msa0708800 AT5G14550.4 31.915 188 96 8 93 268 132 299 2.15e-13 70.1
Msa0708800 AT1G73810.1 29.442 197 104 9 82 264 202 377 7.85e-13 68.9
Msa0708800 AT1G62305.3 34.375 160 55 7 93 223 137 275 1.30e-12 67.8
Msa0708800 AT1G11940.2 39.669 121 49 5 84 202 133 231 2.62e-11 63.5

Find 138 sgRNAs with CRISPR-Local

Find 289 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAAGAGAGATGAAAGAATT+TGG 0.208741 4_4:-82519387 None:intergenic
GCACTGTAGAGAATTGATTT+TGG 0.231426 4_4:-82522574 None:intergenic
AGTGAATGTGAAGATATTTA+TGG 0.243978 4_4:+82521866 Msa0708800:CDS
TTCCAAAAGTGTACTTCTTT+TGG 0.258576 4_4:+82522209 Msa0708800:three_prime_UTR
TCCTTTCCTTAGGGCCTGTT+TGG 0.276015 4_4:+82522525 Msa0708800:three_prime_UTR
ATCTTTGAAAATAACAGATT+TGG 0.276902 4_4:-82520275 None:intergenic
GACCAAAAGAAGTACACTTT+TGG 0.291622 4_4:-82522211 None:intergenic
TCGGTCCATCAGAGAGTTTC+CGG 0.296790 4_4:-82521980 None:intergenic
TAAACATTGAATAATTTAGA+TGG 0.309104 4_4:-82519500 None:intergenic
GAGGATTCTGATGGTGTTAC+TGG 0.315065 4_4:+82520076 Msa0708800:CDS
GGTAGGCGGCCAATGGAATT+AGG 0.322962 4_4:-82522367 None:intergenic
GCTTCATGGAGAATTGCTTT+TGG 0.327793 4_4:-82522658 None:intergenic
TCTCCTTGAACAACGTGTAT+AGG 0.329808 4_4:+82522716 Msa0708800:three_prime_UTR
ATTACCTAAACAATCTTCTA+TGG 0.349395 4_4:-82522129 None:intergenic
AATTGGACTGGGACTGTTAA+TGG 0.353305 4_4:+82520118 Msa0708800:CDS
ATTTCAACCGAACACCGAAT+TGG 0.361586 4_4:-82519799 None:intergenic
ATTCAAGAAAACAGAGAGAT+AGG 0.361657 4_4:+82522028 Msa0708800:three_prime_UTR
ATTGCCATAGAAGATTGTTT+AGG 0.362687 4_4:+82522125 Msa0708800:three_prime_UTR
CTGAACCAAACACGTTGTTT+TGG 0.366733 4_4:-82522612 None:intergenic
ATACACTTACCAATGTGAAT+TGG 0.369149 4_4:+82520101 Msa0708800:CDS
AAAATAGAACAGTTGCAAAA+AGG 0.371617 4_4:-82522331 None:intergenic
AGTATCAGATTCCTTTCCTT+AGG 0.375122 4_4:+82522515 Msa0708800:three_prime_UTR
TCAACAATGGTCCTAGGTTA+TGG 0.378887 4_4:+82519843 Msa0708800:CDS
TCTAGCCAAAACAACGTGTT+TGG 0.385121 4_4:+82522607 Msa0708800:three_prime_UTR
TCAGAGAGTTTCCGGTATAT+AGG 0.389892 4_4:-82521972 None:intergenic
TTTCAACCGAACACCGAATT+GGG 0.399194 4_4:-82519798 None:intergenic
GAAATTTGTTCCTGATTGCT+TGG 0.406788 4_4:+82520234 Msa0708800:CDS
GATTCCAGAGAGGGGTGAAA+TGG 0.407050 4_4:-82519460 None:intergenic
CGTTTGAGAAATTTCGTGTT+GGG 0.409497 4_4:+82519912 Msa0708800:CDS
AGCTAGGATTTATAGCTTCA+TGG 0.411891 4_4:-82522672 None:intergenic
AATCGACCCAATTCGGTGTT+CGG 0.414363 4_4:+82519792 Msa0708800:CDS
AACGTTTGATTTAACCGATT+CGG 0.415173 4_4:+82519769 Msa0708800:CDS
GATTCGGAATCGACCCAATT+CGG 0.415724 4_4:+82519785 Msa0708800:CDS
GAAGAAAAGTAAAAGGAGAA+AGG 0.415911 4_4:-82522389 None:intergenic
TTTGACAAGAAAACATGCTT+TGG 0.419279 4_4:+82519949 Msa0708800:CDS
AAGAAAAGTAAAAGGAGAAA+GGG 0.422906 4_4:-82522388 None:intergenic
TGATTGCTTGGAACCATTGA+TGG 0.426338 4_4:+82520246 Msa0708800:CDS
AATTTCTCAAACGGAACTTC+CGG 0.431140 4_4:-82519902 None:intergenic
AGGGGTGTGAAGAGGAAGAA+GGG 0.436253 4_4:-82519364 None:intergenic
ATCTCCATTTCACCCCTCTC+TGG 0.436718 4_4:+82519456 Msa0708800:CDS
GTATCAGATTCCTTTCCTTA+GGG 0.437137 4_4:+82522516 Msa0708800:three_prime_UTR
ATCAATGGTTCCAAGCAATC+AGG 0.437224 4_4:-82520244 None:intergenic
CATGTGATCACTGTCTTTCG+TGG 0.444520 4_4:+82522434 Msa0708800:three_prime_UTR
TTATGGCTGAGAGATGATAT+TGG 0.455789 4_4:+82521883 Msa0708800:exon
GATCGGTGTTGGTGAGGAAA+AGG 0.455897 4_4:-82519436 None:intergenic
TTTCTGGCTCCTAATTCCAT+TGG 0.461024 4_4:+82522358 Msa0708800:three_prime_UTR
TTGCAGAATGCACAAGCAAT+TGG 0.464082 4_4:-82522303 None:intergenic
AAACATTGAATAATTTAGAT+GGG 0.466588 4_4:-82519499 None:intergenic
ATGGAATTAGGAGCCAGAAA+AGG 0.466672 4_4:-82522355 None:intergenic
GTTGAGAAAGTCGAATAATT+CGG 0.467142 4_4:+82520192 Msa0708800:CDS
TGGAATTAGGAGCCAGAAAA+GGG 0.468006 4_4:-82522354 None:intergenic
ATCAATGCTACCTCATGTTC+AGG 0.468588 4_4:-82522252 None:intergenic
GACGGTACAAATTTGAAGAT+GGG 0.469772 4_4:-82519581 None:intergenic
GAGGGGTGTGAAGAGGAAGA+AGG 0.470582 4_4:-82519365 None:intergenic
TCTCAGAAGTGGAACCAAAC+AGG 0.472317 4_4:-82522539 None:intergenic
AGACGGTACAAATTTGAAGA+TGG 0.472395 4_4:-82519582 None:intergenic
CCGTTTGAGAAATTTCGTGT+TGG 0.473342 4_4:+82519911 Msa0708800:CDS
GGGTGTGAAGAGGAAGAAGG+GGG 0.474839 4_4:-82519362 None:intergenic
GGGGTGTGAAGAGGAAGAAG+GGG 0.477919 4_4:-82519363 None:intergenic
AAATTATCAACAATGGTCCT+AGG 0.486241 4_4:+82519837 Msa0708800:CDS
CTTACCAATGTGAATTGGAC+TGG 0.493929 4_4:+82520106 Msa0708800:CDS
AATTATTCGACTTTCTCAAC+CGG 0.502661 4_4:-82520189 None:intergenic
AAACCTATACACGTTGTTCA+AGG 0.504530 4_4:-82522719 None:intergenic
ATATTGTGGCTATAGCTTGA+TGG 0.506894 4_4:+82521912 Msa0708800:three_prime_UTR
GATTTGGCAATACCCATCAA+TGG 0.512678 4_4:-82520259 None:intergenic
GGATCGCTGAAGCAAGTAAA+CGG 0.513510 4_4:-82519649 None:intergenic
TCCTACGTTGTTGTCTATGG+AGG 0.514638 4_4:+82520057 Msa0708800:CDS
GTAGGAAAATAATGTTCCTC+AGG 0.514706 4_4:-82520040 None:intergenic
CTTTAATTTCATTATGTGTG+TGG 0.518438 4_4:+82522489 Msa0708800:three_prime_UTR
GGTGCTCTTCTATATTCAGT+TGG 0.519705 4_4:+82522079 Msa0708800:three_prime_UTR
AAGAACGATGAAATAAGCAT+TGG 0.522467 4_4:-82519684 None:intergenic
AGTACTACTCCATCTTATAG+TGG 0.523035 4_4:+82522466 Msa0708800:three_prime_UTR
TCCTCCGGCTGATACGTATG+AGG 0.524726 4_4:-82520145 None:intergenic
CGCGTGGCGGAGATTAAAGT+GGG 0.528482 4_4:-82519626 None:intergenic
GGTTAACGCGCGGGTCGGAA+TGG 0.528505 4_4:-82519526 None:intergenic
AAATTAAAGCCACTATAAGA+TGG 0.531212 4_4:-82522475 None:intergenic
GAGAGATGAAAGAATTTGGA+GGG 0.534851 4_4:-82519383 None:intergenic
GCGCGTGGCGGAGATTAAAG+TGG 0.534851 4_4:-82519627 None:intergenic
AGAGAGATGAAAGAATTTGG+AGG 0.536586 4_4:-82519384 None:intergenic
GAATTTGGAGGGGTGTGAAG+AGG 0.541017 4_4:-82519372 None:intergenic
TTTATTGAAATTATCAACAA+TGG 0.541878 4_4:+82519830 Msa0708800:CDS
GGTGAAATGGAGATCGGTGT+TGG 0.542512 4_4:-82519447 None:intergenic
GTGGAACCAAACAGGCCCTA+AGG 0.543878 4_4:-82522531 None:intergenic
CACTCATCATCACGGTAACA+AGG 0.544806 4_4:-82520013 None:intergenic
CTAGAATTGCTTCTCAGAAG+TGG 0.545241 4_4:-82522550 None:intergenic
CCAACACGAAATTTCTCAAA+CGG 0.545393 4_4:-82519911 None:intergenic
TGGACGTTACGCGATGATGC+CGG 0.547095 4_4:+82519883 Msa0708800:CDS
GATATTGGTTGATGATATTG+TGG 0.548010 4_4:+82521898 Msa0708800:three_prime_UTR
TCAGTTGGTGCAATGCAAGT+TGG 0.550891 4_4:+82522094 Msa0708800:three_prime_UTR
TATAATCAAACGAATGAAGA+GGG 0.552046 4_4:-82519721 None:intergenic
TAGTTTGAAGAAGCAATGTA+CGG 0.554306 4_4:+82521942 Msa0708800:three_prime_UTR
GAGAAAGGGTAGGCGGCCAA+TGG 0.555828 4_4:-82522374 None:intergenic
GAATTGGGTCGATTCCGAAT+CGG 0.559523 4_4:-82519783 None:intergenic
AACATTGAATAATTTAGATG+GGG 0.564738 4_4:-82519498 None:intergenic
AAAGTAAAAGGAGAAAGGGT+AGG 0.564744 4_4:-82522384 None:intergenic
GTGAAATAGCGGTGTTGAAG+AGG 0.564970 4_4:-82519331 None:intergenic
GAAAGAAGAGATTCCAGAGA+GGG 0.567482 4_4:-82519469 None:intergenic
TTGTCTATGGAGGATTCTGA+TGG 0.567511 4_4:+82520067 Msa0708800:CDS
ACCAAACAGGCCCTAAGGAA+AGG 0.571034 4_4:-82522526 None:intergenic
CCATCCTCATACGTATCAGC+CGG 0.571067 4_4:+82520141 Msa0708800:CDS
ACGGTACAAATTTGAAGATG+GGG 0.571190 4_4:-82519580 None:intergenic
CGTGAGGTTAACGCGCGGGT+CGG 0.572231 4_4:-82519531 None:intergenic
ATATACCGGAAACTCTCTGA+TGG 0.573662 4_4:+82521975 Msa0708800:three_prime_UTR
ACGGGTCACAACCTATATAC+CGG 0.576622 4_4:+82521961 Msa0708800:three_prime_UTR
TTCTAGAAAAGCTAGAAGCT+AGG 0.580059 4_4:-82522688 None:intergenic
GATTGCTTGGAACCATTGAT+GGG 0.586536 4_4:+82520247 Msa0708800:CDS
AGAGATGAAAGAATTTGGAG+GGG 0.587496 4_4:-82519382 None:intergenic
TGAAGCAAGTAAACGGCGCG+TGG 0.594303 4_4:-82519642 None:intergenic
TGAAAGAAGAGATTCCAGAG+AGG 0.594543 4_4:-82519470 None:intergenic
AGCTCCGGTGACACTTCCTC+CGG 0.595229 4_4:-82520160 None:intergenic
CTCAACCGGAGAATAAGCTC+CGG 0.595442 4_4:-82520175 None:intergenic
TGGAAGCGTTACACGGCGCG+TGG 0.597375 4_4:+82519863 Msa0708800:CDS
CCGGCTGATACGTATGAGGA+TGG 0.599049 4_4:-82520141 None:intergenic
GAGAGGGGTGAAATGGAGAT+CGG 0.601775 4_4:-82519453 None:intergenic
TCAGCCGGAGGAAGTGTCAC+CGG 0.606036 4_4:+82520156 Msa0708800:CDS
AGTTTGAAGAAGCAATGTAC+GGG 0.606738 4_4:+82521943 Msa0708800:three_prime_UTR
TGTCACCGGAGCTTATTCTC+CGG 0.607381 4_4:+82520170 Msa0708800:CDS
ATATAATCAAACGAATGAAG+AGG 0.616226 4_4:-82519722 None:intergenic
TTACCAATGTGAATTGGACT+GGG 0.620634 4_4:+82520107 Msa0708800:CDS
AGCAAGTAAACGGCGCGTGG+CGG 0.624709 4_4:-82519639 None:intergenic
GGGTGTAGAATGTGAAATAG+CGG 0.628873 4_4:-82519342 None:intergenic
GAATCGGTTAAATCAAACGT+TGG 0.632414 4_4:-82519767 None:intergenic
AATGGAGATCGGTGTTGGTG+AGG 0.638764 4_4:-82519442 None:intergenic
CATCATTAATGAAATAGAGT+CGG 0.643658 4_4:-82521999 None:intergenic
GTGTAACGCTTCCATAACCT+AGG 0.647995 4_4:-82519854 None:intergenic
TAGGTTATGGAAGCGTTACA+CGG 0.650942 4_4:+82519856 Msa0708800:CDS
AAAGAAGAGATTCCAGAGAG+GGG 0.653261 4_4:-82519468 None:intergenic
TCCTCATACGTATCAGCCGG+AGG 0.654016 4_4:+82520144 Msa0708800:CDS
TGAGATGTTGCCTGAACATG+AGG 0.664333 4_4:+82522242 Msa0708800:three_prime_UTR
AGTCCCAGTCCAATTCACAT+TGG 0.674151 4_4:-82520110 None:intergenic
CAGGATAACACTCATCATCA+CGG 0.676655 4_4:-82520021 None:intergenic
CGTGGCGGAGATTAAAGTGG+GGG 0.678137 4_4:-82519624 None:intergenic
GTAAAAGGAGAAAGGGTAGG+CGG 0.690764 4_4:-82522381 None:intergenic
AAGCATTGGAAGCATCGTCG+AGG 0.706280 4_4:-82519670 None:intergenic
TTGTGGCTATAGCTTGATGG+CGG 0.723637 4_4:+82521915 Msa0708800:three_prime_UTR
GCGTGGCGGAGATTAAAGTG+GGG 0.740739 4_4:-82519625 None:intergenic
TCCTCCATAGACAACAACGT+AGG 0.744754 4_4:-82520058 None:intergenic
TGATGATGAGTGTTATCCTG+AGG 0.777255 4_4:+82520024 Msa0708800:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAACATTGAATAATTTAGAT+GGG - chr4_4:82519502-82519521 None:intergenic 15.0%
!! TAAACATTGAATAATTTAGA+TGG - chr4_4:82519503-82519522 None:intergenic 15.0%
!! TTTATTGAAATTATCAACAA+TGG + chr4_4:82519830-82519849 Msa0708800:CDS 15.0%
!!! TCTAGAATAATATTCTAATA+GGG - chr4_4:82521691-82521710 None:intergenic 15.0%
!!! ATCTAGAATAATATTCTAAT+AGG - chr4_4:82521692-82521711 None:intergenic 15.0%
!! AAAAATATTCTGTTCAAATT+TGG + chr4_4:82522280-82522299 Msa0708800:three_prime_UTR 15.0%
!! AACATTGAATAATTTAGATG+GGG - chr4_4:82519501-82519520 None:intergenic 20.0%
!!! ATCTTTGAAAATAACAGATT+TGG - chr4_4:82520278-82520297 None:intergenic 20.0%
!!! ATTTTCAAAGATTGAAACTT+TGG + chr4_4:82520286-82520305 Msa0708800:intron 20.0%
!!! TTTTCAAAGATTGAAACTTT+GGG + chr4_4:82520287-82520306 Msa0708800:intron 20.0%
!!! ATTTTCCTTTTTGTTATTGA+TGG + chr4_4:82520373-82520392 Msa0708800:intron 20.0%
!!! TTTTCCTTTTTGTTATTGAT+GGG + chr4_4:82520374-82520393 Msa0708800:intron 20.0%
!! TTATGTTAGAAATTAGAATC+TGG + chr4_4:82520461-82520480 Msa0708800:intron 20.0%
!! TATGTTAGAAATTAGAATCT+GGG + chr4_4:82520462-82520481 Msa0708800:intron 20.0%
!!! TCAAATTACTTTTTGTTTGT+TGG + chr4_4:82520543-82520562 Msa0708800:intron 20.0%
!!! TTTACTTAGAAATTTGGAAT+GGG + chr4_4:82520826-82520845 Msa0708800:intron 20.0%
!! TATTTGGTATCCAAAAATTT+AGG - chr4_4:82521588-82521607 None:intergenic 20.0%
!!! CTTTTACTTTTCTTCTTTTT+GGG + chr4_4:82522397-82522416 Msa0708800:three_prime_UTR 20.0%
! TATAATCAAACGAATGAAGA+GGG - chr4_4:82519724-82519743 None:intergenic 25.0%
! ATATAATCAAACGAATGAAG+AGG - chr4_4:82519725-82519744 None:intergenic 25.0%
! TTTCAAAGATTGAAACTTTG+GGG + chr4_4:82520288-82520307 Msa0708800:intron 25.0%
! GAAATTTCAAGATCTTGATA+TGG + chr4_4:82520312-82520331 Msa0708800:intron 25.0%
! AGTTAGTTATGAGAAAAGTT+TGG + chr4_4:82520400-82520419 Msa0708800:intron 25.0%
!! AAGTTTGGAACTTTATTTGA+TGG + chr4_4:82520415-82520434 Msa0708800:intron 25.0%
!! AGTTTGGAACTTTATTTGAT+GGG + chr4_4:82520416-82520435 Msa0708800:intron 25.0%
! TTTGATGGGAATTATTTATC+AGG + chr4_4:82520430-82520449 Msa0708800:intron 25.0%
!!! TTTTTGTTTGTTGGAATCAA+AGG + chr4_4:82520552-82520571 Msa0708800:intron 25.0%
!!! ATTTGAGATTTTGACATTTG+AGG + chr4_4:82520577-82520596 Msa0708800:intron 25.0%
!!! TTTGAGGTTTAGAATTTTGA+AGG + chr4_4:82520593-82520612 Msa0708800:intron 25.0%
!!! TTGAGGTTTAGAATTTTGAA+GGG + chr4_4:82520594-82520613 Msa0708800:intron 25.0%
! ATTTCTAAGTAAAGCACAAA+TGG - chr4_4:82520819-82520838 None:intergenic 25.0%
! TTGTGCTTTACTTAGAAATT+TGG + chr4_4:82520820-82520839 Msa0708800:intron 25.0%
! CTTTACTTAGAAATTTGGAA+TGG + chr4_4:82520825-82520844 Msa0708800:intron 25.0%
!! TTACTTAGAAATTTGGAATG+GGG + chr4_4:82520827-82520846 Msa0708800:intron 25.0%
!!! ATAATTGCTGTTTGTATTTG+TGG + chr4_4:82520873-82520892 Msa0708800:intron 25.0%
!!! TGCATTTACTTTTTTCTAGT+TGG + chr4_4:82521146-82521165 Msa0708800:intron 25.0%
!!! GCATTTACTTTTTTCTAGTT+GGG + chr4_4:82521147-82521166 Msa0708800:intron 25.0%
! CAAATGTCAAAATCTCAAAT+TGG - chr4_4:82520577-82520596 None:intergenic 25.0%
!!! ATTTGAGATTTTGACATTTG+AGG + chr4_4:82520577-82520596 Msa0708800:intron 25.0%
!! TTTGAGGATTAGAATTTTGA+AGG + chr4_4:82521197-82521216 Msa0708800:intron 25.0%
!! TTGAGGATTAGAATTTTGAA+GGG + chr4_4:82521198-82521217 Msa0708800:intron 25.0%
! TTTGTGCTTACTTAGAAATT+AGG + chr4_4:82521268-82521287 Msa0708800:intron 25.0%
! CTTACTTAGAAATTAGGAAT+GGG + chr4_4:82521274-82521293 Msa0708800:intron 25.0%
! TTACTTAGAAATTAGGAATG+GGG + chr4_4:82521275-82521294 Msa0708800:intron 25.0%
!!! ATAATTGTTGTTTGTGTATG+TGG + chr4_4:82521321-82521340 Msa0708800:intron 25.0%
!!! TAGTTGGTTTATCTTCTTTT+TGG + chr4_4:82521412-82521431 Msa0708800:intron 25.0%
! TTCATATCTATATCAGGATT+TGG - chr4_4:82521473-82521492 None:intergenic 25.0%
! GAATCTGATTAAGATACTAA+TGG - chr4_4:82521786-82521805 None:intergenic 25.0%
! AGTGAATGTGAAGATATTTA+TGG + chr4_4:82521866-82521885 Msa0708800:CDS 25.0%
! CATCATTAATGAAATAGAGT+CGG - chr4_4:82522002-82522021 None:intergenic 25.0%
! ATGATGAGTAAAAAAAAGAG+AGG + chr4_4:82522054-82522073 Msa0708800:three_prime_UTR 25.0%
! ATTACCTAAACAATCTTCTA+TGG - chr4_4:82522132-82522151 None:intergenic 25.0%
! AAAATAGAACAGTTGCAAAA+AGG - chr4_4:82522334-82522353 None:intergenic 25.0%
! AAGAAAAGTAAAAGGAGAAA+GGG - chr4_4:82522391-82522410 None:intergenic 25.0%
! CCAAAAAGAAGAAAAGTAAA+AGG - chr4_4:82522399-82522418 None:intergenic 25.0%
!!! CCTTTTACTTTTCTTCTTTT+TGG + chr4_4:82522396-82522415 Msa0708800:three_prime_UTR 25.0%
! AAATTAAAGCCACTATAAGA+TGG - chr4_4:82522478-82522497 None:intergenic 25.0%
!! CTTTAATTTCATTATGTGTG+TGG + chr4_4:82522489-82522508 Msa0708800:three_prime_UTR 25.0%
AAGAACGATGAAATAAGCAT+TGG - chr4_4:82519687-82519706 None:intergenic 30.0%
! AACGTTTGATTTAACCGATT+CGG + chr4_4:82519769-82519788 Msa0708800:CDS 30.0%
AAATTATCAACAATGGTCCT+AGG + chr4_4:82519837-82519856 Msa0708800:CDS 30.0%
TTTGACAAGAAAACATGCTT+TGG + chr4_4:82519949-82519968 Msa0708800:CDS 30.0%
ATACACTTACCAATGTGAAT+TGG + chr4_4:82520101-82520120 Msa0708800:CDS 30.0%
AATTATTCGACTTTCTCAAC+CGG - chr4_4:82520192-82520211 None:intergenic 30.0%
GTTGAGAAAGTCGAATAATT+CGG + chr4_4:82520192-82520211 Msa0708800:CDS 30.0%
TTCAAAGATTGAAACTTTGG+GGG + chr4_4:82520289-82520308 Msa0708800:intron 30.0%
TGAACCCATCAATAACAAAA+AGG - chr4_4:82520381-82520400 None:intergenic 30.0%
! AAACACTCCTTGATGTTTTT+AGG + chr4_4:82520513-82520532 Msa0708800:intron 30.0%
!! AACACTCCTTGATGTTTTTA+GGG + chr4_4:82520514-82520533 Msa0708800:intron 30.0%
!! TTTAGAATTTTGAAGGGACT+TGG + chr4_4:82520600-82520619 Msa0708800:intron 30.0%
TTGTATTTGTGGTGAGAAAA+TGG + chr4_4:82520884-82520903 Msa0708800:intron 30.0%
TTGCAAATTAACTGCTAATG+AGG - chr4_4:82521077-82521096 None:intergenic 30.0%
!! AGTTAATTTGCAACTATCTC+TGG + chr4_4:82521084-82521103 Msa0708800:intron 30.0%
TAGGTGGTTTATCTTCTTTA+CGG + chr4_4:82521118-82521137 Msa0708800:intron 30.0%
GCTTACTTAGAAATTAGGAA+TGG + chr4_4:82521273-82521292 Msa0708800:intron 30.0%
TTGTGGTGAAAAAATGGTTT+AGG + chr4_4:82521354-82521373 Msa0708800:intron 30.0%
! TACTGTGTGTTGATATGAAA+TGG + chr4_4:82521380-82521399 Msa0708800:intron 30.0%
ATCCAAAAATTTAGGTGAGT+AGG - chr4_4:82521580-82521599 None:intergenic 30.0%
!!! CCACTCTAGTTTTTTCTATT+TGG - chr4_4:82521604-82521623 None:intergenic 30.0%
CCAAATAGAAAAAACTAGAG+TGG + chr4_4:82521601-82521620 Msa0708800:intron 30.0%
ATAGAAAAAACTAGAGTGGA+TGG + chr4_4:82521605-82521624 Msa0708800:intron 30.0%
! AATCTGAAGTGTTATGCTTA+TGG + chr4_4:82521646-82521665 Msa0708800:intron 30.0%
TGGAAACAAAATCTAACACA+AGG - chr4_4:82521766-82521785 None:intergenic 30.0%
!!! ATTCACTGAACTTTTTCATC+TGG - chr4_4:82521853-82521872 None:intergenic 30.0%
!! GATATTGGTTGATGATATTG+TGG + chr4_4:82521898-82521917 Msa0708800:three_prime_UTR 30.0%
TAGTTTGAAGAAGCAATGTA+CGG + chr4_4:82521942-82521961 Msa0708800:three_prime_UTR 30.0%
ATTCAAGAAAACAGAGAGAT+AGG + chr4_4:82522028-82522047 Msa0708800:three_prime_UTR 30.0%
ATTGCCATAGAAGATTGTTT+AGG + chr4_4:82522125-82522144 Msa0708800:three_prime_UTR 30.0%
! CTTCTCTGATTTTTGTACAT+AGG + chr4_4:82522157-82522176 Msa0708800:three_prime_UTR 30.0%
!! TTCCAAAAGTGTACTTCTTT+TGG + chr4_4:82522209-82522228 Msa0708800:three_prime_UTR 30.0%
GAAGAAAAGTAAAAGGAGAA+AGG - chr4_4:82522392-82522411 None:intergenic 30.0%
!! CACTGTAGAGAATTGATTTT+GGG - chr4_4:82522576-82522595 None:intergenic 30.0%
AGAGATGAAAGAATTTGGAG+GGG - chr4_4:82519385-82519404 None:intergenic 35.0%
GAGAGATGAAAGAATTTGGA+GGG - chr4_4:82519386-82519405 None:intergenic 35.0%
AGAGAGATGAAAGAATTTGG+AGG - chr4_4:82519387-82519406 None:intergenic 35.0%
GGAAGAGAGATGAAAGAATT+TGG - chr4_4:82519390-82519409 None:intergenic 35.0%
!! GATTTTGAAGGTAGGGTTTT+TGG - chr4_4:82519411-82519430 None:intergenic 35.0%
! GAAAAGGAAAGCGATTTTGA+AGG - chr4_4:82519423-82519442 None:intergenic 35.0%
! GGTTGTAACTGTTGCTTAAA+CGG - chr4_4:82519562-82519581 None:intergenic 35.0%
ACGGTACAAATTTGAAGATG+GGG - chr4_4:82519583-82519602 None:intergenic 35.0%
GACGGTACAAATTTGAAGAT+GGG - chr4_4:82519584-82519603 None:intergenic 35.0%
AGACGGTACAAATTTGAAGA+TGG - chr4_4:82519585-82519604 None:intergenic 35.0%
GAATCGGTTAAATCAAACGT+TGG - chr4_4:82519770-82519789 None:intergenic 35.0%
AATTTCTCAAACGGAACTTC+CGG - chr4_4:82519905-82519924 None:intergenic 35.0%
CCAACACGAAATTTCTCAAA+CGG - chr4_4:82519914-82519933 None:intergenic 35.0%
CGTTTGAGAAATTTCGTGTT+GGG + chr4_4:82519912-82519931 Msa0708800:CDS 35.0%
! ACATGCTTTGGTTGTTGTTA+AGG + chr4_4:82519961-82519980 Msa0708800:intron 35.0%
! CTTTGGTTGTTGTTAAGGAT+AGG + chr4_4:82519966-82519985 Msa0708800:intron 35.0%
TTGTTAAGGATAGGACATTG+TGG + chr4_4:82519975-82519994 Msa0708800:intron 35.0%
GTAGGAAAATAATGTTCCTC+AGG - chr4_4:82520043-82520062 None:intergenic 35.0%
! TTTTCCTACGTTGTTGTCTA+TGG + chr4_4:82520054-82520073 Msa0708800:CDS 35.0%
TTACCAATGTGAATTGGACT+GGG + chr4_4:82520107-82520126 Msa0708800:CDS 35.0%
! GAAATTTGTTCCTGATTGCT+TGG + chr4_4:82520234-82520253 Msa0708800:CDS 35.0%
TCAAAGATTGAAACTTTGGG+GGG + chr4_4:82520290-82520309 Msa0708800:intron 35.0%
!!! ACACTCCTTGATGTTTTTAG+GGG + chr4_4:82520515-82520534 Msa0708800:intron 35.0%
ACAAACCCCTAAAAACATCA+AGG - chr4_4:82520523-82520542 None:intergenic 35.0%
CAATCAATGCATTGCAAGAT+CGG - chr4_4:82520753-82520772 None:intergenic 35.0%
TTGTGGTGAGAAAATGGTTT+AGG + chr4_4:82520890-82520909 Msa0708800:intron 35.0%
! GTGTTCTGAATGTCAAAACA+TGG + chr4_4:82520920-82520939 Msa0708800:intron 35.0%
!! ACATGGTGCTAGAATCTTTT+AGG + chr4_4:82520937-82520956 Msa0708800:intron 35.0%
CAGAATTTAAACTCACATCG+TGG - chr4_4:82521039-82521058 None:intergenic 35.0%
TATGTGGTATTCATGCTTTG+TGG + chr4_4:82521337-82521356 Msa0708800:intron 35.0%
CATGCTTTGTGGTGAAAAAA+TGG + chr4_4:82521348-82521367 Msa0708800:intron 35.0%
GAAATGGCTAGAATTGTAGT+TGG + chr4_4:82521396-82521415 Msa0708800:intron 35.0%
CAGGTCTTCATATCTATATC+AGG - chr4_4:82521479-82521498 None:intergenic 35.0%
AGATATGAAGACCTGATTAG+TGG + chr4_4:82521484-82521503 Msa0708800:intron 35.0%
GATATGAAGACCTGATTAGT+GGG + chr4_4:82521485-82521504 Msa0708800:intron 35.0%
! GACCTACTCACCTAAATTTT+TGG + chr4_4:82521575-82521594 Msa0708800:intron 35.0%
TTCTGTAATCAGTTTGGACT+TGG - chr4_4:82521734-82521753 None:intergenic 35.0%
ACAAAATCTAACACAAGGAC+TGG - chr4_4:82521761-82521780 None:intergenic 35.0%
TTATGGCTGAGAGATGATAT+TGG + chr4_4:82521883-82521902 Msa0708800:exon 35.0%
ATATTGTGGCTATAGCTTGA+TGG + chr4_4:82521912-82521931 Msa0708800:three_prime_UTR 35.0%
AGTTTGAAGAAGCAATGTAC+GGG + chr4_4:82521943-82521962 Msa0708800:three_prime_UTR 35.0%
TGAGTAAAAAAAAGAGAGGC+AGG + chr4_4:82522058-82522077 Msa0708800:three_prime_UTR 35.0%
!! GACCAAAAGAAGTACACTTT+TGG - chr4_4:82522214-82522233 None:intergenic 35.0%
! CTGTTCTATTTTCCCTTTTC+TGG + chr4_4:82522342-82522361 Msa0708800:three_prime_UTR 35.0%
AAAGTAAAAGGAGAAAGGGT+AGG - chr4_4:82522387-82522406 None:intergenic 35.0%
AGTACTACTCCATCTTATAG+TGG + chr4_4:82522466-82522485 Msa0708800:three_prime_UTR 35.0%
AGTATCAGATTCCTTTCCTT+AGG + chr4_4:82522515-82522534 Msa0708800:three_prime_UTR 35.0%
GTATCAGATTCCTTTCCTTA+GGG + chr4_4:82522516-82522535 Msa0708800:three_prime_UTR 35.0%
!! GCACTGTAGAGAATTGATTT+TGG - chr4_4:82522577-82522596 None:intergenic 35.0%
!!! GTTTTGGCTAGAATTGCTTT+TGG - chr4_4:82522599-82522618 None:intergenic 35.0%
AGCTAGGATTTATAGCTTCA+TGG - chr4_4:82522675-82522694 None:intergenic 35.0%
! TTCTAGAAAAGCTAGAAGCT+AGG - chr4_4:82522691-82522710 None:intergenic 35.0%
AAACCTATACACGTTGTTCA+AGG - chr4_4:82522722-82522741 None:intergenic 35.0%
GGGTGTAGAATGTGAAATAG+CGG - chr4_4:82519345-82519364 None:intergenic 40.0%
!! GGAAAGCGATTTTGAAGGTA+GGG - chr4_4:82519418-82519437 None:intergenic 40.0%
!! AGGAAAGCGATTTTGAAGGT+AGG - chr4_4:82519419-82519438 None:intergenic 40.0%
AAAGAAGAGATTCCAGAGAG+GGG - chr4_4:82519471-82519490 None:intergenic 40.0%
GAAAGAAGAGATTCCAGAGA+GGG - chr4_4:82519472-82519491 None:intergenic 40.0%
TGAAAGAAGAGATTCCAGAG+AGG - chr4_4:82519473-82519492 None:intergenic 40.0%
! TGTAACTGTTGCTTAAACGG+AGG - chr4_4:82519559-82519578 None:intergenic 40.0%
TTTCAACCGAACACCGAATT+GGG - chr4_4:82519801-82519820 None:intergenic 40.0%
ATTTCAACCGAACACCGAAT+TGG - chr4_4:82519802-82519821 None:intergenic 40.0%
TCAACAATGGTCCTAGGTTA+TGG + chr4_4:82519843-82519862 Msa0708800:CDS 40.0%
!! TAGGTTATGGAAGCGTTACA+CGG + chr4_4:82519856-82519875 Msa0708800:CDS 40.0%
CCGTTTGAGAAATTTCGTGT+TGG + chr4_4:82519911-82519930 Msa0708800:CDS 40.0%
TTAAGGATAGGACATTGTGG+AGG + chr4_4:82519978-82519997 Msa0708800:intron 40.0%
GACATTGTGGAGGAAGTTTA+AGG + chr4_4:82519988-82520007 Msa0708800:intron 40.0%
CAGGATAACACTCATCATCA+CGG - chr4_4:82520024-82520043 None:intergenic 40.0%
! TGATGATGAGTGTTATCCTG+AGG + chr4_4:82520024-82520043 Msa0708800:CDS 40.0%
TTGTCTATGGAGGATTCTGA+TGG + chr4_4:82520067-82520086 Msa0708800:CDS 40.0%
CTTACCAATGTGAATTGGAC+TGG + chr4_4:82520106-82520125 Msa0708800:CDS 40.0%
! AATTGGACTGGGACTGTTAA+TGG + chr4_4:82520118-82520137 Msa0708800:CDS 40.0%
GCAATCAGGAACAAATTTCC+TGG - chr4_4:82520233-82520252 None:intergenic 40.0%
ATCAATGGTTCCAAGCAATC+AGG - chr4_4:82520247-82520266 None:intergenic 40.0%
TGATTGCTTGGAACCATTGA+TGG + chr4_4:82520246-82520265 Msa0708800:CDS 40.0%
GATTGCTTGGAACCATTGAT+GGG + chr4_4:82520247-82520266 Msa0708800:CDS 40.0%
GATTTGGCAATACCCATCAA+TGG - chr4_4:82520262-82520281 None:intergenic 40.0%
TGGAGTTGCTTCTTAATGTG+TGG + chr4_4:82520620-82520639 Msa0708800:intron 40.0%
AGTTGCTTCTTAATGTGTGG+AGG + chr4_4:82520623-82520642 Msa0708800:intron 40.0%
! TTCTTAATGTGTGGAGGTGT+GGG + chr4_4:82520629-82520648 Msa0708800:intron 40.0%
ACACTCACTAGTTGACCATA+GGG - chr4_4:82520664-82520683 None:intergenic 40.0%
TACACTCACTAGTTGACCAT+AGG - chr4_4:82520665-82520684 None:intergenic 40.0%
TATGGTCAACTAGTGAGTGT+AGG + chr4_4:82520664-82520683 Msa0708800:intron 40.0%
TAGTGAGTGTAGGTGAATCT+CGG + chr4_4:82520674-82520693 Msa0708800:intron 40.0%
! GATCTGTTGCTGATTACAGA+GGG + chr4_4:82520849-82520868 Msa0708800:intron 40.0%
!! TGGTGCTAGAATCTTTTAGG+TGG + chr4_4:82520940-82520959 Msa0708800:intron 40.0%
GAGTAGATGCTAACTACAGT+TGG + chr4_4:82520985-82521004 Msa0708800:intron 40.0%
TGAGCTGTATCCTTACCAAT+CGG - chr4_4:82521012-82521031 None:intergenic 40.0%
! ATCTCTGGTGCTAGAATCTT+AGG + chr4_4:82521099-82521118 Msa0708800:intron 40.0%
! TCGAGTTGCTTCTTATTGTG+TGG + chr4_4:82521224-82521243 Msa0708800:intron 40.0%
AGTTGCTTCTTATTGTGTGG+TGG + chr4_4:82521227-82521246 Msa0708800:intron 40.0%
! AACTAGAGTACTAGATGCTG+AGG + chr4_4:82521439-82521458 Msa0708800:intron 40.0%
TTGCAGATTACCCACTAATC+AGG - chr4_4:82521498-82521517 None:intergenic 40.0%
!! ACCTATGACAGCGCTTTTTA+AGG + chr4_4:82521520-82521539 Msa0708800:intron 40.0%
!! ACCTTAAAAAGCGCTGTCAT+AGG - chr4_4:82521524-82521543 None:intergenic 40.0%
GAAAAAACTAGAGTGGATGG+TGG + chr4_4:82521608-82521627 Msa0708800:intron 40.0%
!! TAGAGTGGATGGTGGATATT+GGG + chr4_4:82521616-82521635 Msa0708800:intron 40.0%
AGTGTTATGCTTATGGACAG+TGG + chr4_4:82521653-82521672 Msa0708800:intron 40.0%
GGCAAGTTCTGTAATCAGTT+TGG - chr4_4:82521740-82521759 None:intergenic 40.0%
TCAGAGAGTTTCCGGTATAT+AGG - chr4_4:82521975-82521994 None:intergenic 40.0%
ATATACCGGAAACTCTCTGA+TGG + chr4_4:82521975-82521994 Msa0708800:three_prime_UTR 40.0%
GGTGCTCTTCTATATTCAGT+TGG + chr4_4:82522079-82522098 Msa0708800:three_prime_UTR 40.0%
ATCAATGCTACCTCATGTTC+AGG - chr4_4:82522255-82522274 None:intergenic 40.0%
TTGCAGAATGCACAAGCAAT+TGG - chr4_4:82522306-82522325 None:intergenic 40.0%
TGGAATTAGGAGCCAGAAAA+GGG - chr4_4:82522357-82522376 None:intergenic 40.0%
ATGGAATTAGGAGCCAGAAA+AGG - chr4_4:82522358-82522377 None:intergenic 40.0%
!! TTTCTGGCTCCTAATTCCAT+TGG + chr4_4:82522358-82522377 Msa0708800:three_prime_UTR 40.0%
CTAGAATTGCTTCTCAGAAG+TGG - chr4_4:82522553-82522572 None:intergenic 40.0%
TCTAGCCAAAACAACGTGTT+TGG + chr4_4:82522607-82522626 Msa0708800:three_prime_UTR 40.0%
! CTGAACCAAACACGTTGTTT+TGG - chr4_4:82522615-82522634 None:intergenic 40.0%
!! TCATGGAGAATTGCTTTTGG+AGG - chr4_4:82522658-82522677 None:intergenic 40.0%
! GCTTCATGGAGAATTGCTTT+TGG - chr4_4:82522661-82522680 None:intergenic 40.0%
TCTCCTTGAACAACGTGTAT+AGG + chr4_4:82522716-82522735 Msa0708800:three_prime_UTR 40.0%
! GTGAAATAGCGGTGTTGAAG+AGG - chr4_4:82519334-82519353 None:intergenic 45.0%
! GCACGGTAGGTTTTCTTAGA+CGG - chr4_4:82519602-82519621 None:intergenic 45.0%
AGATTAAAGTGGGGGAAGCA+CGG - chr4_4:82519619-82519638 None:intergenic 45.0%
GGATCGCTGAAGCAAGTAAA+CGG - chr4_4:82519652-82519671 None:intergenic 45.0%
! GAATTGGGTCGATTCCGAAT+CGG - chr4_4:82519786-82519805 None:intergenic 45.0%
GATTCGGAATCGACCCAATT+CGG + chr4_4:82519785-82519804 Msa0708800:CDS 45.0%
!! AATCGACCCAATTCGGTGTT+CGG + chr4_4:82519792-82519811 Msa0708800:CDS 45.0%
GTGTAACGCTTCCATAACCT+AGG - chr4_4:82519857-82519876 None:intergenic 45.0%
CACTCATCATCACGGTAACA+AGG - chr4_4:82520016-82520035 None:intergenic 45.0%
TCCTACGTTGTTGTCTATGG+AGG + chr4_4:82520057-82520076 Msa0708800:CDS 45.0%
TCCTCCATAGACAACAACGT+AGG - chr4_4:82520061-82520080 None:intergenic 45.0%
!! GAGGATTCTGATGGTGTTAC+TGG + chr4_4:82520076-82520095 Msa0708800:CDS 45.0%
AGTCCCAGTCCAATTCACAT+TGG - chr4_4:82520113-82520132 None:intergenic 45.0%
!!! CGGAGAGTTTTTTGTTTGCC+AGG + chr4_4:82520212-82520231 Msa0708800:intron 45.0%
CTTCTTAATGTGTGGAGGTG+TGG + chr4_4:82520628-82520647 Msa0708800:intron 45.0%
! TGTGGGAAATGGATTCCCTA+TGG + chr4_4:82520646-82520665 Msa0708800:intron 45.0%
CAATGCATTGCAAGATCGGA+GGG - chr4_4:82520749-82520768 None:intergenic 45.0%
TCAATGCATTGCAAGATCGG+AGG - chr4_4:82520750-82520769 None:intergenic 45.0%
GATTGAGTGACTGCAGAATC+CGG + chr4_4:82520768-82520787 Msa0708800:intron 45.0%
! GGATCTGTTGCTGATTACAG+AGG + chr4_4:82520848-82520867 Msa0708800:intron 45.0%
CTAACTACAGTTGGTCCGAT+TGG + chr4_4:82520994-82521013 Msa0708800:intron 45.0%
TACAGTTGGTCCGATTGGTA+AGG + chr4_4:82520999-82521018 Msa0708800:intron 45.0%
! TCTGGTGCTAGAATCTTAGG+TGG + chr4_4:82521102-82521121 Msa0708800:intron 45.0%
TGCTTCTTATTGTGTGGTGG+TGG + chr4_4:82521230-82521249 Msa0708800:intron 45.0%
! TGGGGTTCTGTTGCTGATTA+TGG + chr4_4:82521293-82521312 Msa0708800:intron 45.0%
GGTGTGACATGTCTTGATCT+TGG + chr4_4:82521541-82521560 Msa0708800:intron 45.0%
GACATGTCTTGATCTTGGTG+AGG + chr4_4:82521546-82521565 Msa0708800:intron 45.0%
CTTGATCTTGGTGAGGTTCT+AGG + chr4_4:82521553-82521572 Msa0708800:intron 45.0%
!! CTAGAGTGGATGGTGGATAT+TGG + chr4_4:82521615-82521634 Msa0708800:intron 45.0%
TTGTGGCTATAGCTTGATGG+CGG + chr4_4:82521915-82521934 Msa0708800:three_prime_UTR 45.0%
ACGGGTCACAACCTATATAC+CGG + chr4_4:82521961-82521980 Msa0708800:three_prime_UTR 45.0%
! TCAGTTGGTGCAATGCAAGT+TGG + chr4_4:82522094-82522113 Msa0708800:three_prime_UTR 45.0%
TGAGATGTTGCCTGAACATG+AGG + chr4_4:82522242-82522261 Msa0708800:three_prime_UTR 45.0%
GTAAAAGGAGAAAGGGTAGG+CGG - chr4_4:82522384-82522403 None:intergenic 45.0%
CATGTGATCACTGTCTTTCG+TGG + chr4_4:82522434-82522453 Msa0708800:three_prime_UTR 45.0%
TCTCAGAAGTGGAACCAAAC+AGG - chr4_4:82522542-82522561 None:intergenic 45.0%
! AGGGGTGTGAAGAGGAAGAA+GGG - chr4_4:82519367-82519386 None:intergenic 50.0%
! GAATTTGGAGGGGTGTGAAG+AGG - chr4_4:82519375-82519394 None:intergenic 50.0%
!! GATCGGTGTTGGTGAGGAAA+AGG - chr4_4:82519439-82519458 None:intergenic 50.0%
!! AATGGAGATCGGTGTTGGTG+AGG - chr4_4:82519445-82519464 None:intergenic 50.0%
GGTGAAATGGAGATCGGTGT+TGG - chr4_4:82519450-82519469 None:intergenic 50.0%
GAGAGGGGTGAAATGGAGAT+CGG - chr4_4:82519456-82519475 None:intergenic 50.0%
ATCTCCATTTCACCCCTCTC+TGG + chr4_4:82519456-82519475 Msa0708800:CDS 50.0%
GATTCCAGAGAGGGGTGAAA+TGG - chr4_4:82519463-82519482 None:intergenic 50.0%
!! AAGCATTGGAAGCATCGTCG+AGG - chr4_4:82519673-82519692 None:intergenic 50.0%
CCATCCTCATACGTATCAGC+CGG + chr4_4:82520141-82520160 Msa0708800:CDS 50.0%
TGTCACCGGAGCTTATTCTC+CGG + chr4_4:82520170-82520189 Msa0708800:CDS 50.0%
CTCAACCGGAGAATAAGCTC+CGG - chr4_4:82520178-82520197 None:intergenic 50.0%
! ATGTGTGGAGGTGTGGGAAA+TGG + chr4_4:82520635-82520654 Msa0708800:intron 50.0%
CATTGCAAGATCGGAGGGTA+CGG - chr4_4:82520744-82520763 None:intergenic 50.0%
TCGGTCCATCAGAGAGTTTC+CGG - chr4_4:82521983-82522002 None:intergenic 50.0%
TCCTTTCCTTAGGGCCTGTT+TGG + chr4_4:82522525-82522544 Msa0708800:three_prime_UTR 50.0%
ACCAAACAGGCCCTAAGGAA+AGG - chr4_4:82522529-82522548 None:intergenic 50.0%
GGGTGTGAAGAGGAAGAAGG+GGG - chr4_4:82519365-82519384 None:intergenic 55.0%
GGGGTGTGAAGAGGAAGAAG+GGG - chr4_4:82519366-82519385 None:intergenic 55.0%
! GAGGGGTGTGAAGAGGAAGA+AGG - chr4_4:82519368-82519387 None:intergenic 55.0%
! TAAAGTGGGGGAAGCACGGT+AGG - chr4_4:82519615-82519634 None:intergenic 55.0%
! CGTGGCGGAGATTAAAGTGG+GGG - chr4_4:82519627-82519646 None:intergenic 55.0%
! GCGTGGCGGAGATTAAAGTG+GGG - chr4_4:82519628-82519647 None:intergenic 55.0%
! CGCGTGGCGGAGATTAAAGT+GGG - chr4_4:82519629-82519648 None:intergenic 55.0%
TGAAGCAAGTAAACGGCGCG+TGG - chr4_4:82519645-82519664 None:intergenic 55.0%
TGGACGTTACGCGATGATGC+CGG + chr4_4:82519883-82519902 Msa0708800:CDS 55.0%
CCGGCTGATACGTATGAGGA+TGG - chr4_4:82520144-82520163 None:intergenic 55.0%
TCCTCATACGTATCAGCCGG+AGG + chr4_4:82520144-82520163 Msa0708800:CDS 55.0%
TCCTCCGGCTGATACGTATG+AGG - chr4_4:82520148-82520167 None:intergenic 55.0%
GGTAGGCGGCCAATGGAATT+AGG - chr4_4:82522370-82522389 None:intergenic 55.0%
GTGGAACCAAACAGGCCCTA+AGG - chr4_4:82522534-82522553 None:intergenic 55.0%
! TGCTTAAACGGAGGCGCGTG+AGG - chr4_4:82519550-82519569 None:intergenic 60.0%
! GCGCGTGGCGGAGATTAAAG+TGG - chr4_4:82519630-82519649 None:intergenic 60.0%
AGCAAGTAAACGGCGCGTGG+CGG - chr4_4:82519642-82519661 None:intergenic 60.0%
!! TCAGCCGGAGGAAGTGTCAC+CGG + chr4_4:82520156-82520175 Msa0708800:CDS 60.0%
AGCTCCGGTGACACTTCCTC+CGG - chr4_4:82520163-82520182 None:intergenic 60.0%
GTGACTGCAGAATCCGGCTC+CGG + chr4_4:82520774-82520793 Msa0708800:intron 60.0%
GAGAAAGGGTAGGCGGCCAA+TGG - chr4_4:82522377-82522396 None:intergenic 60.0%
GGTTAACGCGCGGGTCGGAA+TGG - chr4_4:82519529-82519548 None:intergenic 65.0%
CGTGAGGTTAACGCGCGGGT+CGG - chr4_4:82519534-82519553 None:intergenic 65.0%
AGGCGCGTGAGGTTAACGCG+CGG - chr4_4:82519539-82519558 None:intergenic 65.0%
!! TGGAAGCGTTACACGGCGCG+TGG + chr4_4:82519863-82519882 Msa0708800:CDS 65.0%
ACTGCAGAATCCGGCTCCGG+TGG + chr4_4:82520777-82520796 Msa0708800:intron 65.0%
AGAATCCGGCTCCGGTGGTG+TGG + chr4_4:82520782-82520801 Msa0708800:intron 65.0%
GAATCCGGCTCCGGTGGTGT+GGG + chr4_4:82520783-82520802 Msa0708800:intron 65.0%
ACTTCGCACGCCCACACCAC+CGG - chr4_4:82520796-82520815 None:intergenic 65.0%
GGCGCGTGAGGTTAACGCGC+GGG - chr4_4:82519538-82519557 None:intergenic 70.0%
CACGCCCACACCACCGGAGC+CGG - chr4_4:82520790-82520809 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 82519321 82522783 82519321 ID=Msa0708800;Name=Msa0708800
chr4_4 mRNA 82519321 82522783 82519321 ID=Msa0708800-mRNA-1;Parent=Msa0708800;Name=Msa0708800-mRNA-1;_AED=0.12;_eAED=0.23;_QI=0|0|0.16|1|0|0|6|891|301
chr4_4 exon 82519321 82519541 82519321 ID=Msa0708800-mRNA-1:exon:27149;Parent=Msa0708800-mRNA-1
chr4_4 exon 82519574 82519813 82519574 ID=Msa0708800-mRNA-1:exon:27150;Parent=Msa0708800-mRNA-1
chr4_4 exon 82519829 82519970 82519829 ID=Msa0708800-mRNA-1:exon:27151;Parent=Msa0708800-mRNA-1
chr4_4 exon 82520010 82520224 82520010 ID=Msa0708800-mRNA-1:exon:27152;Parent=Msa0708800-mRNA-1
chr4_4 exon 82520234 82520282 82520234 ID=Msa0708800-mRNA-1:exon:27153;Parent=Msa0708800-mRNA-1
chr4_4 exon 82521854 82522783 82521854 ID=Msa0708800-mRNA-1:exon:27154;Parent=Msa0708800-mRNA-1
chr4_4 CDS 82519321 82519541 82519321 ID=Msa0708800-mRNA-1:cds;Parent=Msa0708800-mRNA-1
chr4_4 CDS 82519574 82519813 82519574 ID=Msa0708800-mRNA-1:cds;Parent=Msa0708800-mRNA-1
chr4_4 CDS 82519829 82519970 82519829 ID=Msa0708800-mRNA-1:cds;Parent=Msa0708800-mRNA-1
chr4_4 CDS 82520010 82520224 82520010 ID=Msa0708800-mRNA-1:cds;Parent=Msa0708800-mRNA-1
chr4_4 CDS 82520234 82520282 82520234 ID=Msa0708800-mRNA-1:cds;Parent=Msa0708800-mRNA-1
chr4_4 CDS 82521854 82521892 82521854 ID=Msa0708800-mRNA-1:cds;Parent=Msa0708800-mRNA-1
chr4_4 three_prime_UTR 82521893 82522783 82521893 ID=Msa0708800-mRNA-1:three_prime_utr;Parent=Msa0708800-mRNA-1
Gene Sequence

>Msa0708800

ATGACATCAACCTCTTCAACACCGCTATTTCACATTCTACACCCCCTTCTTCCTCTTCACACCCCTCCAAATTCTTTCATCTCTCTTCCAAAAACCCTACCTTCAAAATCGCTTTCCTTTTCCTCACCAACACCGATCTCCATTTCACCCCTCTCTGGAATCTCTTCTTTCAAACAACCCCATCTAAATTATTCAATGTTTATGTCCATTCCGACCCGCGCTTACAACCCCATCTTCAAATTTGTACCGTCTAAGAAAACCTACCGTGCTTCCCCCACTTTAATCTCCGCCACGCGCCGTTTACTTGCTTCAGCGATCCTCGACGATGCTTCCAATGCTTATTTCATCGTTCTTTCGCAATATTGTATCCCTCTTCATTCGTTTGATTATATCTATAAATCACTTTTTCTTTCACCAACGTTTGATTTAACCGATTCGGAATCGACCCAATTCGGTGTTCGCTTTATTGAAATTATCAACAATGGTCCTAGGTTATGGAAGCGTTACACGGCGCGTGGACGTTACGCGATGATGCCGGAAGTTCCGTTTGAGAAATTTCGTGTTGGGTCGCAGTTTTTTACTTTGACAAGAAAACATGCTTTGGTTCCTTGTTACCGTGATGATGAGTGTTATCCTGAGGAACATTATTTTCCTACGTTGTTGTCTATGGAGGATTCTGATGGTGTTACTGGCTATACACTTACCAATGTGAATTGGACTGGGACTGTTAATGGCCATCCTCATACGTATCAGCCGGAGGAAGTGTCACCGGAGCTTATTCTCCGGTTGAGAAAGTCGAATAATTCGGAGAGTTTTTTGAAATTTGTTCCTGATTGCTTGGAACCATTGATGGGTATTGCCAAATCTATGAAAAAGTTCAGTGAATGTGAAGATATTTATGGCTGA
Protein sequence

>Msa0708800

MTSTSSTPLFHILHPLLPLHTPPNSFISLPKTLPSKSLSFSSPTPISISPLSGISSFKQPHLNYSMFMSIPTRAYNPIFKFVPSKKTYRASPTLISATRRLLASAILDDASNAYFIVLSQYCIPLHSFDYIYKSLFLSPTFDLTDSESTQFGVRFIEIINNGPRLWKRYTARGRYAMMPEVPFEKFRVGSQFFTLTRKHALVPCYRDDECYPEEHYFPTLLSMEDSDGVTGYTLTNVNWTGTVNGHPHTYQPEEVSPELILRLRKSNNSESFLKFVPDCLEPLMGIAKSMKKFSECEDIYG*