AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0712640

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0712640 MtrunA17_Chr5g0397281 91.743 218 18 0 1 218 1 218 6.32e-149 416
Msa0712640 MtrunA17_Chr3g0126191 33.824 136 90 0 73 208 76 211 3.03e-24 96.7
Msa0712640 MtrunA17_Chr7g0221181 29.560 159 110 1 57 213 50 208 1.36e-23 94.4
Msa0712640 MtrunA17_Chr2g0289851 24.138 174 120 5 39 201 41 213 1.76e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0712640 AT5G41470.1 48.148 135 70 0 74 208 66 200 6.60e-38 133
Msa0712640 AT1G71480.1 32.576 132 89 0 81 212 69 200 9.29e-26 100
Msa0712640 AT1G71480.3 33.333 105 70 0 81 185 69 173 6.23e-23 91.7
Msa0712640 AT1G71480.2 28.916 83 59 0 81 163 69 151 2.73e-12 62.8

Find 81 sgRNAs with CRISPR-Local

Find 246 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAAACTCTGGTTGGGAATT+TGG 0.130689 5_1:+4482052 None:intergenic
CCACCACTTCCTCCTGTTAT+AGG 0.246729 5_1:+4480641 Msa0712630:CDS
TGACTATGAATTTCACATTC+TGG 0.276043 5_1:+4481548 None:intergenic
CAAACCTTCTTTCCTTGAAA+TGG 0.293968 5_1:+4481708 None:intergenic
ATGCTTGTACTTCAGATCTA+AGG 0.302459 5_1:+4480193 Msa0712630:intron
TGGCATGGTAGAATGGAAAA+AGG 0.312351 5_1:-4481384 Msa0712640:intron
TTGACGGCTGAGTTTATTCC+CGG 0.318740 5_1:+4482020 None:intergenic
GACCTCATGCAATACTATTA+TGG 0.322975 5_1:-4480893 Msa0712640:three_prime_UTR
CTGAGTTTATTCCCGGATAA+TGG 0.326204 5_1:+4482027 None:intergenic
ATTTAACTGTTGCGGCAAAA+TGG 0.329724 5_1:-4481504 Msa0712640:CDS
TAGAAGAAAACATGACAATT+AGG 0.335380 5_1:-4481313 Msa0712640:intron
TCCTCCTGTTATAGGATCAT+TGG 0.342440 5_1:+4480649 Msa0712630:CDS
GACTCACATTCAGCTGCATT+TGG 0.356576 5_1:+4481005 None:intergenic
GTTACCAAAACTATGCTTCC+AGG 0.364460 5_1:+4481181 None:intergenic
TTACAACACTTGAAAGAGTT+AGG 0.368447 5_1:+4481145 None:intergenic
CCCTCTCGTTGTTGGATACA+TGG 0.370228 5_1:+4480675 Msa0712630:CDS
CAGAGTTTCATGCCATTATC+CGG 0.370820 5_1:-4482039 Msa0712640:CDS
GGCCGGCTATAGGCGACTAT+TGG 0.377649 5_1:-4480617 Msa0712640:three_prime_UTR
AGCTATCTAGTAGTGGATTA+AGG 0.384056 5_1:+4480835 None:intergenic
AAAGGAAACAAAAGTACAAT+TGG 0.393314 5_1:+4481106 None:intergenic
ACCTTCATCAATCCATTTCA+AGG 0.398951 5_1:-4481720 Msa0712640:CDS
ATTGGTTGCCCTCTCGTTGT+TGG 0.399335 5_1:+4480667 Msa0712630:CDS
CAAACCTGGAAGCATAGTTT+TGG 0.405840 5_1:-4481185 Msa0712640:CDS
CAATCCATTTCAAGGAAAGA+AGG 0.408899 5_1:-4481712 Msa0712640:intron
AAGTGGTGGAGGCCGGCTAT+AGG 0.418164 5_1:-4480627 Msa0712640:three_prime_UTR
TTCTTACAGCAGCTTACTGC+TGG 0.418688 5_1:-4481577 Msa0712640:CDS
AGGAACATGCATGTATGTTA+AGG 0.419513 5_1:+4480932 None:intergenic
TATAGTTACTCACCTGCAAT+TGG 0.426851 5_1:-4480227 Msa0712640:three_prime_UTR
AAAGATGAATCTGGCGAGAT+GGG 0.432844 5_1:+4480569 Msa0712630:CDS
CGGATAATGGCATGAAACTC+TGG 0.438289 5_1:+4482040 None:intergenic
GCAACCAATGATCCTATAAC+AGG 0.441435 5_1:-4480653 Msa0712640:three_prime_UTR
ACAGCAGCTTACTGCTGGCA+TGG 0.459854 5_1:-4481572 Msa0712640:CDS
GCCGGCTATAGGCGACTATT+GGG 0.460498 5_1:-4480616 Msa0712640:three_prime_UTR
ACATACATGCATGTTCCTGC+AGG 0.462917 5_1:-4480927 Msa0712640:three_prime_UTR
TCCTTGAAATGGATTGATGA+AGG 0.467392 5_1:+4481719 None:intergenic
ACCAATTGAATCAATCCAAA+AGG 0.487395 5_1:+4481088 None:intergenic
TACGTGTCAGTATCGTGTCT+TGG 0.489349 5_1:+4480136 Msa0712630:intron
GATCCATTCACTTGGTAGAC+CGG 0.491216 5_1:-4480319 Msa0712640:three_prime_UTR
GGAACATGCATGTATGTTAA+GGG 0.494614 5_1:+4480933 None:intergenic
GAACAAATCCCATTCACAAC+AGG 0.495206 5_1:-4481362 Msa0712640:CDS
AAGTGATCTACTATCTCTGC+TGG 0.496660 5_1:+4481819 None:intergenic
AGCTGCAGCCTGTTGTGAAT+GGG 0.499013 5_1:+4481354 None:intergenic
AAGCTGCAGCCTGTTGTGAA+TGG 0.506105 5_1:+4481353 None:intergenic
AAGCCGGTCTACCAAGTGAA+TGG 0.508284 5_1:+4480316 Msa0712630:intron
CTAAGACTATATTTAAGTAG+AGG 0.515504 5_1:-4480746 Msa0712640:three_prime_UTR
AATGGCATGAAACTCTGGTT+GGG 0.526617 5_1:+4482045 None:intergenic
AAAAGATGAATCTGGCGAGA+TGG 0.530203 5_1:+4480568 Msa0712630:CDS
GATCCTATAACAGGAGGAAG+TGG 0.536218 5_1:-4480644 Msa0712640:three_prime_UTR
CAGGAGGAAGTGGTGGAGGC+CGG 0.536906 5_1:-4480634 Msa0712640:three_prime_UTR
CAGCAGCTTACTGCTGGCAT+GGG 0.537521 5_1:-4481571 Msa0712640:CDS
TAATGGCATGAAACTCTGGT+TGG 0.544699 5_1:+4482044 None:intergenic
AGAGTTTCATGCCATTATCC+GGG 0.545323 5_1:-4482038 Msa0712640:CDS
ACCCAATAGTCGCCTATAGC+CGG 0.547078 5_1:+4480615 Msa0712630:CDS
GGGAGATGATTTAACTGTTG+CGG 0.550185 5_1:-4481512 Msa0712640:CDS
CTACCAAGTGAATGGATCAC+AGG 0.559874 5_1:+4480324 Msa0712630:intron
AGAAACATTACAAATGGATG+TGG 0.563651 5_1:-4482078 None:intergenic
TAGATAGCTACATAAAGCTA+TGG 0.564545 5_1:-4480821 Msa0712640:three_prime_UTR
TGCTGTCCTCATCAGTGCTG+CGG 0.577568 5_1:-4480703 Msa0712640:three_prime_UTR
GTCATCAAACAATGAAGTCA+CGG 0.578442 5_1:+4481031 None:intergenic
ATAACAGGAGGAAGTGGTGG+AGG 0.586799 5_1:-4480638 Msa0712640:three_prime_UTR
ATTGAATCACTAGTCAAACC+TGG 0.588394 5_1:-4481199 Msa0712640:CDS
CCTATAACAGGAGGAAGTGG+TGG 0.596015 5_1:-4480641 Msa0712640:three_prime_UTR
GTGGAACCGCAGCACTGATG+AGG 0.597213 5_1:+4480697 Msa0712630:CDS
GTTGCGGCAAAATGGCACTT+AGG 0.602225 5_1:-4481496 Msa0712640:intron
GGTCTTCTTAAGAACCCTGC+AGG 0.602800 5_1:+4480912 None:intergenic
TCTAGTAGTGGATTAAGGAA+AGG 0.610402 5_1:+4480840 None:intergenic
TTTATGTAGCTATCTAGTAG+TGG 0.616283 5_1:+4480828 None:intergenic
TACCCTGTGATCCATTCACT+TGG 0.621091 5_1:-4480327 Msa0712640:three_prime_UTR
CATACATGCATGTTCCTGCA+GGG 0.632925 5_1:-4480926 Msa0712640:three_prime_UTR
ATAGTCAGAAATATATGCGA+GGG 0.636276 5_1:-4481532 Msa0712640:CDS
AAGATGAATCTGGCGAGATG+GGG 0.637726 5_1:+4480570 Msa0712630:CDS
ATCCATAATAGTATTGCATG+AGG 0.639529 5_1:+4480891 None:intergenic
CATAGTCAGAAATATATGCG+AGG 0.642978 5_1:-4481533 Msa0712640:CDS
ACCAATGATCCTATAACAGG+AGG 0.648824 5_1:-4480650 Msa0712640:three_prime_UTR
ACCATGTATCCAACAACGAG+AGG 0.650347 5_1:-4480676 Msa0712640:three_prime_UTR
CCATGTATCCAACAACGAGA+GGG 0.655825 5_1:-4480675 Msa0712640:three_prime_UTR
AGCTGAATGTGAGTCAAACA+TGG 0.656790 5_1:-4480997 Msa0712640:three_prime_UTR
CTATAGATAAAGTTCATGCG+TGG 0.662656 5_1:+4480253 Msa0712630:intron
TACCAAGTGAATGGATCACA+GGG 0.666568 5_1:+4480325 Msa0712630:intron
TTTGCTTGGCGAGAAACTGA+GGG 0.684286 5_1:+4481986 None:intergenic
TCTCGTTGTTGGATACATGG+TGG 0.695822 5_1:+4480678 Msa0712630:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GCTTATATCATTTATACTTT+AGG + chr5_1:4480762-4480781 None:intergenic 20.0%
!!! TTTCTTCTACTTTTTCTAAT+TGG + chr5_1:4480858-4480877 None:intergenic 20.0%
!!! ATGATATTTTGCATTACTAT+AGG + chr5_1:4481664-4481683 None:intergenic 20.0%
!! GCTTATATCATTTATACTTT+AGG + chr5_1:4480762-4480781 None:intergenic 20.0%
!!! TTTCTTCTACTTTTTCTAAT+TGG + chr5_1:4480858-4480877 None:intergenic 20.0%
!!! ATGATATTTTGCATTACTAT+AGG + chr5_1:4481664-4481683 None:intergenic 20.0%
!! TTTGAATTTTTCCCGAATTT+GGG + chr5_1:4480339-4480358 Msa0712630:intron 25.0%
! TGAAAAAGAGCTAAAACAAT+TGG - chr5_1:4480401-4480420 Msa0712640:intron 25.0%
! TAATCTAAACATAACATTGC+AGG - chr5_1:4480571-4480590 Msa0712640:three_prime_UTR 25.0%
! AAAAGCAATAAATCTAGGAA+AGG + chr5_1:4480719-4480738 None:intergenic 25.0%
! TAGAAGAAAACATGACAATT+AGG - chr5_1:4480869-4480888 Msa0712640:three_prime_UTR 25.0%
! CTCACTATATAATTGAATTC+AGG - chr5_1:4480945-4480964 Msa0712640:three_prime_UTR 25.0%
!! AAAGGAAACAAAAGTACAAT+TGG + chr5_1:4481079-4481098 None:intergenic 25.0%
!!! ATTGTACTTTTGTTTCCTTT+TGG - chr5_1:4481079-4481098 Msa0712640:three_prime_UTR 25.0%
!! GTATGTTAAGGGATATTTTT+GGG + chr5_1:4481241-4481260 None:intergenic 25.0%
!! TGTATGTTAAGGGATATTTT+TGG + chr5_1:4481242-4481261 None:intergenic 25.0%
! CTAAGACTATATTTAAGTAG+AGG - chr5_1:4481436-4481455 Msa0712640:intron 25.0%
! AAACAAGAAAAAGATGAATC+TGG + chr5_1:4481625-4481644 None:intergenic 25.0%
!!! TCATCTTTTTCTTGTTTCTT+AGG - chr5_1:4481628-4481647 Msa0712640:intron 25.0%
! TTGCATTACTATAGGTATAT+TGG + chr5_1:4481656-4481675 None:intergenic 25.0%
!! TTTGAATTTTTCCCGAATTT+GGG + chr5_1:4480339-4480358 Msa0712630:intron 25.0%
! TGAAAAAGAGCTAAAACAAT+TGG - chr5_1:4480401-4480420 Msa0712640:intron 25.0%
! TAATCTAAACATAACATTGC+AGG - chr5_1:4480571-4480590 Msa0712640:three_prime_UTR 25.0%
! AAAAGCAATAAATCTAGGAA+AGG + chr5_1:4480719-4480738 None:intergenic 25.0%
! TAGAAGAAAACATGACAATT+AGG - chr5_1:4480869-4480888 Msa0712640:three_prime_UTR 25.0%
! CTCACTATATAATTGAATTC+AGG - chr5_1:4480945-4480964 Msa0712640:three_prime_UTR 25.0%
!! AAAGGAAACAAAAGTACAAT+TGG + chr5_1:4481079-4481098 None:intergenic 25.0%
!!! ATTGTACTTTTGTTTCCTTT+TGG - chr5_1:4481079-4481098 Msa0712640:three_prime_UTR 25.0%
!! GTATGTTAAGGGATATTTTT+GGG + chr5_1:4481241-4481260 None:intergenic 25.0%
!! TGTATGTTAAGGGATATTTT+TGG + chr5_1:4481242-4481261 None:intergenic 25.0%
! CTAAGACTATATTTAAGTAG+AGG - chr5_1:4481436-4481455 Msa0712640:intron 25.0%
! AAACAAGAAAAAGATGAATC+TGG + chr5_1:4481625-4481644 None:intergenic 25.0%
!!! TCATCTTTTTCTTGTTTCTT+AGG - chr5_1:4481628-4481647 Msa0712640:intron 25.0%
! TTGCATTACTATAGGTATAT+TGG + chr5_1:4481656-4481675 None:intergenic 25.0%
AGAATGTCAAAAACAGAACT+TGG - chr5_1:4480274-4480293 Msa0712640:three_prime_UTR 30.0%
GAATGTCAAAAACAGAACTT+GGG - chr5_1:4480275-4480294 Msa0712640:three_prime_UTR 30.0%
TCAGCCATATTATCAAAAAG+AGG - chr5_1:4480312-4480331 Msa0712640:three_prime_UTR 30.0%
! TTGAATTTTTCCCGAATTTG+GGG + chr5_1:4480338-4480357 Msa0712630:intron 30.0%
! GTTTGAATTTTTCCCGAATT+TGG + chr5_1:4480340-4480359 Msa0712630:intron 30.0%
GTTATTAGAACTTAGAAGGA+TGG + chr5_1:4480508-4480527 Msa0712630:intron 30.0%
CTAGGTTATTAGAACTTAGA+AGG + chr5_1:4480512-4480531 Msa0712630:intron 30.0%
TGACTATGAATTTCACATTC+TGG + chr5_1:4480637-4480656 Msa0712630:CDS 30.0%
ATAGTCAGAAATATATGCGA+GGG - chr5_1:4480650-4480669 Msa0712640:three_prime_UTR 30.0%
AGCAGAAAAGCAATAAATCT+AGG + chr5_1:4480724-4480743 None:intergenic 30.0%
! AAAGAGTTAGGTTCTGTTTT+AGG + chr5_1:4481028-4481047 None:intergenic 30.0%
TTACAACACTTGAAAGAGTT+AGG + chr5_1:4481040-4481059 None:intergenic 30.0%
!! TCCTTTTGGATTGATTCAAT+TGG - chr5_1:4481093-4481112 Msa0712640:three_prime_UTR 30.0%
ACCAATTGAATCAATCCAAA+AGG + chr5_1:4481097-4481116 None:intergenic 30.0%
ATCCATAATAGTATTGCATG+AGG + chr5_1:4481294-4481313 None:intergenic 30.0%
TTTATGTAGCTATCTAGTAG+TGG + chr5_1:4481357-4481376 None:intergenic 30.0%
TAGATAGCTACATAAAGCTA+TGG - chr5_1:4481361-4481380 Msa0712640:CDS 30.0%
!! TAGTTTGAGTGTTGAACAAT+GGG + chr5_1:4481747-4481766 None:intergenic 30.0%
AAGTACAAGCATGAAAAACT+CGG - chr5_1:4482000-4482019 Msa0712640:CDS 30.0%
AGAATGTCAAAAACAGAACT+TGG - chr5_1:4480274-4480293 Msa0712640:three_prime_UTR 30.0%
GAATGTCAAAAACAGAACTT+GGG - chr5_1:4480275-4480294 Msa0712640:three_prime_UTR 30.0%
TCAGCCATATTATCAAAAAG+AGG - chr5_1:4480312-4480331 Msa0712640:three_prime_UTR 30.0%
! TTGAATTTTTCCCGAATTTG+GGG + chr5_1:4480338-4480357 Msa0712630:intron 30.0%
! GTTTGAATTTTTCCCGAATT+TGG + chr5_1:4480340-4480359 Msa0712630:intron 30.0%
GTTATTAGAACTTAGAAGGA+TGG + chr5_1:4480508-4480527 Msa0712630:intron 30.0%
CTAGGTTATTAGAACTTAGA+AGG + chr5_1:4480512-4480531 Msa0712630:intron 30.0%
TGACTATGAATTTCACATTC+TGG + chr5_1:4480637-4480656 Msa0712630:CDS 30.0%
ATAGTCAGAAATATATGCGA+GGG - chr5_1:4480650-4480669 Msa0712640:three_prime_UTR 30.0%
AGCAGAAAAGCAATAAATCT+AGG + chr5_1:4480724-4480743 None:intergenic 30.0%
! AAAGAGTTAGGTTCTGTTTT+AGG + chr5_1:4481028-4481047 None:intergenic 30.0%
TTACAACACTTGAAAGAGTT+AGG + chr5_1:4481040-4481059 None:intergenic 30.0%
!! TCCTTTTGGATTGATTCAAT+TGG - chr5_1:4481093-4481112 Msa0712640:three_prime_UTR 30.0%
ACCAATTGAATCAATCCAAA+AGG + chr5_1:4481097-4481116 None:intergenic 30.0%
ATCCATAATAGTATTGCATG+AGG + chr5_1:4481294-4481313 None:intergenic 30.0%
TTTATGTAGCTATCTAGTAG+TGG + chr5_1:4481357-4481376 None:intergenic 30.0%
TAGATAGCTACATAAAGCTA+TGG - chr5_1:4481361-4481380 Msa0712640:CDS 30.0%
!! TAGTTTGAGTGTTGAACAAT+GGG + chr5_1:4481747-4481766 None:intergenic 30.0%
AAGTACAAGCATGAAAAACT+CGG - chr5_1:4482000-4482019 Msa0712640:CDS 30.0%
!!! AAGTTGCATGTTGTTTTGCT+TGG + chr5_1:4480213-4480232 Msa0712630:intron 35.0%
ACCTTCATCAATCCATTTCA+AGG - chr5_1:4480462-4480481 Msa0712640:intron 35.0%
!! TCCTTGAAATGGATTGATGA+AGG + chr5_1:4480466-4480485 Msa0712630:intron 35.0%
CAATCCATTTCAAGGAAAGA+AGG - chr5_1:4480470-4480489 Msa0712640:intron 35.0%
CAAACCTTCTTTCCTTGAAA+TGG + chr5_1:4480477-4480496 Msa0712630:intron 35.0%
CATAGTCAGAAATATATGCG+AGG - chr5_1:4480649-4480668 Msa0712640:three_prime_UTR 35.0%
ATTTAACTGTTGCGGCAAAA+TGG - chr5_1:4480678-4480697 Msa0712640:three_prime_UTR 35.0%
! AAGCCTGACGAATATCTTTT+TGG - chr5_1:4480778-4480797 Msa0712640:three_prime_UTR 35.0%
ATGCCAAAAAGATATTCGTC+AGG + chr5_1:4480784-4480803 None:intergenic 35.0%
!! TGACGAATATCTTTTTGGCA+TGG - chr5_1:4480783-4480802 Msa0712640:three_prime_UTR 35.0%
!! ATCTTTTTGGCATGGTAGAA+TGG - chr5_1:4480791-4480810 Msa0712640:three_prime_UTR 35.0%
ATTGAATCACTAGTCAAACC+TGG - chr5_1:4480983-4481002 Msa0712640:three_prime_UTR 35.0%
GTCATCAAACAATGAAGTCA+CGG + chr5_1:4481154-4481173 None:intergenic 35.0%
GGAACATGCATGTATGTTAA+GGG + chr5_1:4481252-4481271 None:intergenic 35.0%
AGGAACATGCATGTATGTTA+AGG + chr5_1:4481253-4481272 None:intergenic 35.0%
GACCTCATGCAATACTATTA+TGG - chr5_1:4481289-4481308 Msa0712640:intron 35.0%
!! TCTAGTAGTGGATTAAGGAA+AGG + chr5_1:4481345-4481364 None:intergenic 35.0%
!! AGCTATCTAGTAGTGGATTA+AGG + chr5_1:4481350-4481369 None:intergenic 35.0%
!! CTAGTTTGAGTGTTGAACAA+TGG + chr5_1:4481748-4481767 None:intergenic 35.0%
TGCGAAAAACAGATTTAAGC+CGG + chr5_1:4481885-4481904 None:intergenic 35.0%
CTATAGATAAAGTTCATGCG+TGG + chr5_1:4481932-4481951 None:intergenic 35.0%
TATAGTTACTCACCTGCAAT+TGG - chr5_1:4481955-4481974 Msa0712640:CDS 35.0%
!!! GCTTGTTTTATTCCAATTGC+AGG + chr5_1:4481970-4481989 None:intergenic 35.0%
ATGCTTGTACTTCAGATCTA+AGG + chr5_1:4481992-4482011 None:intergenic 35.0%
!!! AAGTTGCATGTTGTTTTGCT+TGG + chr5_1:4480213-4480232 Msa0712630:intron 35.0%
ACCTTCATCAATCCATTTCA+AGG - chr5_1:4480462-4480481 Msa0712640:intron 35.0%
!! TCCTTGAAATGGATTGATGA+AGG + chr5_1:4480466-4480485 Msa0712630:intron 35.0%
CAATCCATTTCAAGGAAAGA+AGG - chr5_1:4480470-4480489 Msa0712640:intron 35.0%
CAAACCTTCTTTCCTTGAAA+TGG + chr5_1:4480477-4480496 Msa0712630:intron 35.0%
CATAGTCAGAAATATATGCG+AGG - chr5_1:4480649-4480668 Msa0712640:three_prime_UTR 35.0%
ATTTAACTGTTGCGGCAAAA+TGG - chr5_1:4480678-4480697 Msa0712640:three_prime_UTR 35.0%
! AAGCCTGACGAATATCTTTT+TGG - chr5_1:4480778-4480797 Msa0712640:three_prime_UTR 35.0%
ATGCCAAAAAGATATTCGTC+AGG + chr5_1:4480784-4480803 None:intergenic 35.0%
!! TGACGAATATCTTTTTGGCA+TGG - chr5_1:4480783-4480802 Msa0712640:three_prime_UTR 35.0%
!! ATCTTTTTGGCATGGTAGAA+TGG - chr5_1:4480791-4480810 Msa0712640:three_prime_UTR 35.0%
ATTGAATCACTAGTCAAACC+TGG - chr5_1:4480983-4481002 Msa0712640:three_prime_UTR 35.0%
GTCATCAAACAATGAAGTCA+CGG + chr5_1:4481154-4481173 None:intergenic 35.0%
GGAACATGCATGTATGTTAA+GGG + chr5_1:4481252-4481271 None:intergenic 35.0%
AGGAACATGCATGTATGTTA+AGG + chr5_1:4481253-4481272 None:intergenic 35.0%
GACCTCATGCAATACTATTA+TGG - chr5_1:4481289-4481308 Msa0712640:intron 35.0%
!! TCTAGTAGTGGATTAAGGAA+AGG + chr5_1:4481345-4481364 None:intergenic 35.0%
!! AGCTATCTAGTAGTGGATTA+AGG + chr5_1:4481350-4481369 None:intergenic 35.0%
!! CTAGTTTGAGTGTTGAACAA+TGG + chr5_1:4481748-4481767 None:intergenic 35.0%
TGCGAAAAACAGATTTAAGC+CGG + chr5_1:4481885-4481904 None:intergenic 35.0%
CTATAGATAAAGTTCATGCG+TGG + chr5_1:4481932-4481951 None:intergenic 35.0%
TATAGTTACTCACCTGCAAT+TGG - chr5_1:4481955-4481974 Msa0712640:CDS 35.0%
!!! GCTTGTTTTATTCCAATTGC+AGG + chr5_1:4481970-4481989 None:intergenic 35.0%
ATGCTTGTACTTCAGATCTA+AGG + chr5_1:4481992-4482011 None:intergenic 35.0%
TGAAACTCTGGTTGGGAATT+TGG + chr5_1:4480133-4480152 Msa0712630:intron 40.0%
AATGGCATGAAACTCTGGTT+GGG + chr5_1:4480140-4480159 Msa0712630:intron 40.0%
TAATGGCATGAAACTCTGGT+TGG + chr5_1:4480141-4480160 Msa0712630:intron 40.0%
CAGAGTTTCATGCCATTATC+CGG - chr5_1:4480143-4480162 Msa0712640:three_prime_UTR 40.0%
AGAGTTTCATGCCATTATCC+GGG - chr5_1:4480144-4480163 Msa0712640:three_prime_UTR 40.0%
CTGAGTTTATTCCCGGATAA+TGG + chr5_1:4480158-4480177 Msa0712630:intron 40.0%
!! GGGGCCTCTTTTTGATAATA+TGG + chr5_1:4480319-4480338 Msa0712630:intron 40.0%
TCAAAAAGAGGCCCCAAATT+CGG - chr5_1:4480324-4480343 Msa0712640:three_prime_UTR 40.0%
AAGTGATCTACTATCTCTGC+TGG + chr5_1:4480366-4480385 Msa0712630:intron 40.0%
AGCTGTTTACTCGATGCAAT+CGG + chr5_1:4480543-4480562 Msa0712630:CDS 40.0%
GGGAGATGATTTAACTGTTG+CGG - chr5_1:4480670-4480689 Msa0712640:three_prime_UTR 40.0%
TGGCATGGTAGAATGGAAAA+AGG - chr5_1:4480798-4480817 Msa0712640:three_prime_UTR 40.0%
GAACAAATCCCATTCACAAC+AGG - chr5_1:4480820-4480839 Msa0712640:three_prime_UTR 40.0%
!! CAAACCTGGAAGCATAGTTT+TGG - chr5_1:4480997-4481016 Msa0712640:three_prime_UTR 40.0%
GTTACCAAAACTATGCTTCC+AGG + chr5_1:4481004-4481023 None:intergenic 40.0%
AGCTGAATGTGAGTCAAACA+TGG - chr5_1:4481185-4481204 Msa0712640:CDS 40.0%
GCAACCAATGATCCTATAAC+AGG - chr5_1:4481529-4481548 Msa0712640:CDS 40.0%
ACCAATGATCCTATAACAGG+AGG - chr5_1:4481532-4481551 Msa0712640:CDS 40.0%
TCCTCCTGTTATAGGATCAT+TGG + chr5_1:4481536-4481555 None:intergenic 40.0%
AAAGATGAATCTGGCGAGAT+GGG + chr5_1:4481616-4481635 None:intergenic 40.0%
AAAAGATGAATCTGGCGAGA+TGG + chr5_1:4481617-4481636 None:intergenic 40.0%
TACCAAGTGAATGGATCACA+GGG + chr5_1:4481860-4481879 None:intergenic 40.0%
TGAAACTCTGGTTGGGAATT+TGG + chr5_1:4480133-4480152 Msa0712630:intron 40.0%
AATGGCATGAAACTCTGGTT+GGG + chr5_1:4480140-4480159 Msa0712630:intron 40.0%
TAATGGCATGAAACTCTGGT+TGG + chr5_1:4480141-4480160 Msa0712630:intron 40.0%
CAGAGTTTCATGCCATTATC+CGG - chr5_1:4480143-4480162 Msa0712640:three_prime_UTR 40.0%
AGAGTTTCATGCCATTATCC+GGG - chr5_1:4480144-4480163 Msa0712640:three_prime_UTR 40.0%
CTGAGTTTATTCCCGGATAA+TGG + chr5_1:4480158-4480177 Msa0712630:intron 40.0%
!! GGGGCCTCTTTTTGATAATA+TGG + chr5_1:4480319-4480338 Msa0712630:intron 40.0%
TCAAAAAGAGGCCCCAAATT+CGG - chr5_1:4480324-4480343 Msa0712640:three_prime_UTR 40.0%
AAGTGATCTACTATCTCTGC+TGG + chr5_1:4480366-4480385 Msa0712630:intron 40.0%
AGCTGTTTACTCGATGCAAT+CGG + chr5_1:4480543-4480562 Msa0712630:CDS 40.0%
GGGAGATGATTTAACTGTTG+CGG - chr5_1:4480670-4480689 Msa0712640:three_prime_UTR 40.0%
TGGCATGGTAGAATGGAAAA+AGG - chr5_1:4480798-4480817 Msa0712640:three_prime_UTR 40.0%
GAACAAATCCCATTCACAAC+AGG - chr5_1:4480820-4480839 Msa0712640:three_prime_UTR 40.0%
!! CAAACCTGGAAGCATAGTTT+TGG - chr5_1:4480997-4481016 Msa0712640:three_prime_UTR 40.0%
GTTACCAAAACTATGCTTCC+AGG + chr5_1:4481004-4481023 None:intergenic 40.0%
AGCTGAATGTGAGTCAAACA+TGG - chr5_1:4481185-4481204 Msa0712640:CDS 40.0%
GCAACCAATGATCCTATAAC+AGG - chr5_1:4481529-4481548 Msa0712640:CDS 40.0%
ACCAATGATCCTATAACAGG+AGG - chr5_1:4481532-4481551 Msa0712640:CDS 40.0%
TCCTCCTGTTATAGGATCAT+TGG + chr5_1:4481536-4481555 None:intergenic 40.0%
AAAGATGAATCTGGCGAGAT+GGG + chr5_1:4481616-4481635 None:intergenic 40.0%
AAAAGATGAATCTGGCGAGA+TGG + chr5_1:4481617-4481636 None:intergenic 40.0%
TACCAAGTGAATGGATCACA+GGG + chr5_1:4481860-4481879 None:intergenic 40.0%
! CGGATAATGGCATGAAACTC+TGG + chr5_1:4480145-4480164 Msa0712630:intron 45.0%
TTGACGGCTGAGTTTATTCC+CGG + chr5_1:4480165-4480184 Msa0712630:intron 45.0%
! GAGGGTGAGTCTCTTTTTGA+CGG + chr5_1:4480181-4480200 Msa0712630:intron 45.0%
TTTGCTTGGCGAGAAACTGA+GGG + chr5_1:4480199-4480218 Msa0712630:intron 45.0%
! TTTTGCTTGGCGAGAAACTG+AGG + chr5_1:4480200-4480219 Msa0712630:intron 45.0%
CAAAAAGAGGCCCCAAATTC+GGG - chr5_1:4480325-4480344 Msa0712640:three_prime_UTR 45.0%
ATGCAATCGGGGTTACTTCT+AGG + chr5_1:4480530-4480549 Msa0712630:intron 45.0%
CTGTTTACTCGATGCAATCG+GGG + chr5_1:4480541-4480560 Msa0712630:intron 45.0%
GCTGTTTACTCGATGCAATC+GGG + chr5_1:4480542-4480561 Msa0712630:intron 45.0%
TTCTTACAGCAGCTTACTGC+TGG - chr5_1:4480605-4480624 Msa0712640:three_prime_UTR 45.0%
GACTCACATTCAGCTGCATT+TGG + chr5_1:4481180-4481199 None:intergenic 45.0%
ACATACATGCATGTTCCTGC+AGG - chr5_1:4481255-4481274 Msa0712640:intron 45.0%
CATACATGCATGTTCCTGCA+GGG - chr5_1:4481256-4481275 Msa0712640:intron 45.0%
TCTCGTTGTTGGATACATGG+TGG + chr5_1:4481507-4481526 None:intergenic 45.0%
ACCATGTATCCAACAACGAG+AGG - chr5_1:4481506-4481525 Msa0712640:CDS 45.0%
CCATGTATCCAACAACGAGA+GGG - chr5_1:4481507-4481526 Msa0712640:CDS 45.0%
GATCCTATAACAGGAGGAAG+TGG - chr5_1:4481538-4481557 Msa0712640:CDS 45.0%
AAGATGAATCTGGCGAGATG+GGG + chr5_1:4481615-4481634 None:intergenic 45.0%
TACCCTGTGATCCATTCACT+TGG - chr5_1:4481855-4481874 Msa0712640:CDS 45.0%
CTACCAAGTGAATGGATCAC+AGG + chr5_1:4481861-4481880 None:intergenic 45.0%
GATCCATTCACTTGGTAGAC+CGG - chr5_1:4481863-4481882 Msa0712640:CDS 45.0%
TACGTGTCAGTATCGTGTCT+TGG + chr5_1:4482049-4482068 None:intergenic 45.0%
! CGGATAATGGCATGAAACTC+TGG + chr5_1:4480145-4480164 Msa0712630:intron 45.0%
TTGACGGCTGAGTTTATTCC+CGG + chr5_1:4480165-4480184 Msa0712630:intron 45.0%
! GAGGGTGAGTCTCTTTTTGA+CGG + chr5_1:4480181-4480200 Msa0712630:intron 45.0%
TTTGCTTGGCGAGAAACTGA+GGG + chr5_1:4480199-4480218 Msa0712630:intron 45.0%
! TTTTGCTTGGCGAGAAACTG+AGG + chr5_1:4480200-4480219 Msa0712630:intron 45.0%
CAAAAAGAGGCCCCAAATTC+GGG - chr5_1:4480325-4480344 Msa0712640:three_prime_UTR 45.0%
ATGCAATCGGGGTTACTTCT+AGG + chr5_1:4480530-4480549 Msa0712630:intron 45.0%
CTGTTTACTCGATGCAATCG+GGG + chr5_1:4480541-4480560 Msa0712630:intron 45.0%
GCTGTTTACTCGATGCAATC+GGG + chr5_1:4480542-4480561 Msa0712630:intron 45.0%
TTCTTACAGCAGCTTACTGC+TGG - chr5_1:4480605-4480624 Msa0712640:three_prime_UTR 45.0%
GACTCACATTCAGCTGCATT+TGG + chr5_1:4481180-4481199 None:intergenic 45.0%
ACATACATGCATGTTCCTGC+AGG - chr5_1:4481255-4481274 Msa0712640:intron 45.0%
CATACATGCATGTTCCTGCA+GGG - chr5_1:4481256-4481275 Msa0712640:intron 45.0%
TCTCGTTGTTGGATACATGG+TGG + chr5_1:4481507-4481526 None:intergenic 45.0%
ACCATGTATCCAACAACGAG+AGG - chr5_1:4481506-4481525 Msa0712640:CDS 45.0%
CCATGTATCCAACAACGAGA+GGG - chr5_1:4481507-4481526 Msa0712640:CDS 45.0%
GATCCTATAACAGGAGGAAG+TGG - chr5_1:4481538-4481557 Msa0712640:CDS 45.0%
AAGATGAATCTGGCGAGATG+GGG + chr5_1:4481615-4481634 None:intergenic 45.0%
TACCCTGTGATCCATTCACT+TGG - chr5_1:4481855-4481874 Msa0712640:CDS 45.0%
CTACCAAGTGAATGGATCAC+AGG + chr5_1:4481861-4481880 None:intergenic 45.0%
GATCCATTCACTTGGTAGAC+CGG - chr5_1:4481863-4481882 Msa0712640:CDS 45.0%
TACGTGTCAGTATCGTGTCT+TGG + chr5_1:4482049-4482068 None:intergenic 45.0%
GTTGCGGCAAAATGGCACTT+AGG - chr5_1:4480686-4480705 Msa0712640:three_prime_UTR 50.0%
AGCTGCAGCCTGTTGTGAAT+GGG + chr5_1:4480831-4480850 None:intergenic 50.0%
AAGCTGCAGCCTGTTGTGAA+TGG + chr5_1:4480832-4480851 None:intergenic 50.0%
GGTCTTCTTAAGAACCCTGC+AGG + chr5_1:4481273-4481292 None:intergenic 50.0%
CCCTCTCGTTGTTGGATACA+TGG + chr5_1:4481510-4481529 None:intergenic 50.0%
ATTGGTTGCCCTCTCGTTGT+TGG + chr5_1:4481518-4481537 None:intergenic 50.0%
CCACCACTTCCTCCTGTTAT+AGG + chr5_1:4481544-4481563 None:intergenic 50.0%
CCTATAACAGGAGGAAGTGG+TGG - chr5_1:4481541-4481560 Msa0712640:CDS 50.0%
ATAACAGGAGGAAGTGGTGG+AGG - chr5_1:4481544-4481563 Msa0712640:CDS 50.0%
ACCCAATAGTCGCCTATAGC+CGG + chr5_1:4481570-4481589 None:intergenic 50.0%
AAGCCGGTCTACCAAGTGAA+TGG + chr5_1:4481869-4481888 None:intergenic 50.0%
GTTGCGGCAAAATGGCACTT+AGG - chr5_1:4480686-4480705 Msa0712640:three_prime_UTR 50.0%
AGCTGCAGCCTGTTGTGAAT+GGG + chr5_1:4480831-4480850 None:intergenic 50.0%
AAGCTGCAGCCTGTTGTGAA+TGG + chr5_1:4480832-4480851 None:intergenic 50.0%
GGTCTTCTTAAGAACCCTGC+AGG + chr5_1:4481273-4481292 None:intergenic 50.0%
CCCTCTCGTTGTTGGATACA+TGG + chr5_1:4481510-4481529 None:intergenic 50.0%
ATTGGTTGCCCTCTCGTTGT+TGG + chr5_1:4481518-4481537 None:intergenic 50.0%
CCACCACTTCCTCCTGTTAT+AGG + chr5_1:4481544-4481563 None:intergenic 50.0%
CCTATAACAGGAGGAAGTGG+TGG - chr5_1:4481541-4481560 Msa0712640:CDS 50.0%
ATAACAGGAGGAAGTGGTGG+AGG - chr5_1:4481544-4481563 Msa0712640:CDS 50.0%
ACCCAATAGTCGCCTATAGC+CGG + chr5_1:4481570-4481589 None:intergenic 50.0%
AAGCCGGTCTACCAAGTGAA+TGG + chr5_1:4481869-4481888 None:intergenic 50.0%
ACAGCAGCTTACTGCTGGCA+TGG - chr5_1:4480610-4480629 Msa0712640:three_prime_UTR 55.0%
CAGCAGCTTACTGCTGGCAT+GGG - chr5_1:4480611-4480630 Msa0712640:three_prime_UTR 55.0%
TGCTGTCCTCATCAGTGCTG+CGG - chr5_1:4481479-4481498 Msa0712640:intron 55.0%
GCCGGCTATAGGCGACTATT+GGG - chr5_1:4481566-4481585 Msa0712640:CDS 55.0%
ACAGCAGCTTACTGCTGGCA+TGG - chr5_1:4480610-4480629 Msa0712640:three_prime_UTR 55.0%
CAGCAGCTTACTGCTGGCAT+GGG - chr5_1:4480611-4480630 Msa0712640:three_prime_UTR 55.0%
TGCTGTCCTCATCAGTGCTG+CGG - chr5_1:4481479-4481498 Msa0712640:intron 55.0%
GCCGGCTATAGGCGACTATT+GGG - chr5_1:4481566-4481585 Msa0712640:CDS 55.0%
!! GTGGAACCGCAGCACTGATG+AGG + chr5_1:4481488-4481507 None:intergenic 60.0%
! AAGTGGTGGAGGCCGGCTAT+AGG - chr5_1:4481555-4481574 Msa0712640:CDS 60.0%
GGCCGGCTATAGGCGACTAT+TGG - chr5_1:4481565-4481584 Msa0712640:CDS 60.0%
!! GTGGAACCGCAGCACTGATG+AGG + chr5_1:4481488-4481507 None:intergenic 60.0%
! AAGTGGTGGAGGCCGGCTAT+AGG - chr5_1:4481555-4481574 Msa0712640:CDS 60.0%
GGCCGGCTATAGGCGACTAT+TGG - chr5_1:4481565-4481584 Msa0712640:CDS 60.0%
! CAGGAGGAAGTGGTGGAGGC+CGG - chr5_1:4481548-4481567 Msa0712640:CDS 65.0%
! CAGGAGGAAGTGGTGGAGGC+CGG - chr5_1:4481548-4481567 Msa0712640:CDS 65.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 4480117 4482087 4480117 ID=Msa0712640;Name=Msa0712640
chr5_1 mRNA 4480117 4482087 4480117 ID=Msa0712640-mRNA-1;Parent=Msa0712640;Name=Msa0712640-mRNA-1;_AED=0.08;_eAED=0.08;_QI=0|1|0.8|1|0.75|0.6|5|819|225
chr5_1 exon 4480117 4480350 4480117 ID=Msa0712640-mRNA-1:exon:1848;Parent=Msa0712640-mRNA-1
chr5_1 exon 4480547 4481237 4480547 ID=Msa0712640-mRNA-1:exon:1847;Parent=Msa0712640-mRNA-1
chr5_1 exon 4481314 4481395 4481314 ID=Msa0712640-mRNA-1:exon:1846;Parent=Msa0712640-mRNA-1
chr5_1 exon 4481497 4481611 4481497 ID=Msa0712640-mRNA-1:exon:1845;Parent=Msa0712640-mRNA-1
chr5_1 exon 4481713 4482087 4481713 ID=Msa0712640-mRNA-1:exon:1844;Parent=Msa0712640-mRNA-1
chr5_1 CDS 4481713 4482087 4481713 ID=Msa0712640-mRNA-1:cds;Parent=Msa0712640-mRNA-1
chr5_1 CDS 4481497 4481611 4481497 ID=Msa0712640-mRNA-1:cds;Parent=Msa0712640-mRNA-1
chr5_1 CDS 4481314 4481395 4481314 ID=Msa0712640-mRNA-1:cds;Parent=Msa0712640-mRNA-1
chr5_1 CDS 4481132 4481237 4481132 ID=Msa0712640-mRNA-1:cds;Parent=Msa0712640-mRNA-1
chr5_1 three_prime_UTR 4480547 4481131 4480547 ID=Msa0712640-mRNA-1:three_prime_utr;Parent=Msa0712640-mRNA-1
chr5_1 three_prime_UTR 4480117 4480350 4480117 ID=Msa0712640-mRNA-1:three_prime_utr;Parent=Msa0712640-mRNA-1
Gene Sequence

>Msa0712640

ATGGATGTGGTAGCCAAATTCCCAACCAGAGTTTCATGCCATTATCCGGGAATAAACTCAGCCGTCAAAAAGAGACTCACCCTCAGTTTCTCGCCAAGCAAAACAACATGCAACTTGCACAAAAAGATAGAGATACAACAAAGAAGCATTTGTTTCAAGAATGTCAAAAACAGAACTTGGGATAACAATAGAATTTCAGCCATATTATCAAAAAGAGGCCCCAAATTCGGGAAAAATTCAAACTCTCCAGCAGAGATAGTAGATCACTTCTACACATGTATCAATGAAAAAGAGCTAAAACAATTGGATGAATACATATCTCAAGATGCTTGTTTTTATGACTATACCTTCATCAATCCATTTCAAGGAAAGAAGGAAGTGATGCATTTCTTACAGCAGCTTACTGCTGGCATGGGCCAGAATGTGAAATTCATAGTCAGAAATATATGCGAGGGAGATGATTTAACTGTTGCGGCAAAATGGCACTTAGAATGGAAAAAGGAACAAATCCCATTCACAACAGGCTGCAGCTTCTTCCAATTAGAAAAAGTAGAAGAAAACATGACAATTAGGAGAGCTGAGATATTTATTGAATCACTAGTCAAACCTGGAAGCATAGTTTTGGTAACTCCTAAAACAGAACCTAACTCTTTCAAGTGTTGTAATTCTTATACGTGA
Protein sequence

>Msa0712640

MDVVAKFPTRVSCHYPGINSAVKKRLTLSFSPSKTTCNLHKKIEIQQRSICFKNVKNRTWDNNRISAILSKRGPKFGKNSNSPAEIVDHFYTCINEKELKQLDEYISQDACFYDYTFINPFQGKKEVMHFLQQLTAGMGQNVKFIVRNICEGDDLTVAAKWHLEWKKEQIPFTTGCSFFQLEKVEENMTIRRAEIFIESLVKPGSIVLVTPKTEPNSFKCCNSYT*