AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0752870

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0752870 MtrunA17_Chr5g0397281 89.450 218 23 0 1 218 1 218 1.74e-144 404
Msa0752870 MtrunA17_Chr7g0221181 30.882 136 94 0 78 213 73 208 9.72e-24 94.7
Msa0752870 MtrunA17_Chr3g0126191 33.088 136 91 0 73 208 76 211 3.59e-23 94.0
Msa0752870 MtrunA17_Chr2g0289851 23.267 202 135 5 19 201 13 213 1.41e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0752870 AT5G41470.1 49.606 127 64 0 82 208 74 200 1.24e-37 132
Msa0752870 AT1G71480.1 32.576 132 89 0 81 212 69 200 9.69e-26 100
Msa0752870 AT1G71480.3 33.333 105 70 0 81 185 69 173 6.56e-23 91.7
Msa0752870 AT1G71480.2 28.916 83 59 0 81 163 69 151 2.75e-12 62.8

Find 81 sgRNAs with CRISPR-Local

Find 244 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAAACTCTGGTTGGGAATT+TGG 0.130689 5_2:-6586355 None:intergenic
CCACCACTTCCTCCTGTTAT+AGG 0.246729 5_2:-6587766 Msa0752880:CDS
TGACTATGAATTTCACATTC+TGG 0.276043 5_2:-6586859 None:intergenic
CAAACCTTCTTTCCTTGAAA+TGG 0.293968 5_2:-6586699 None:intergenic
ATGCTTGTACTTCAGATCTA+AGG 0.302459 5_2:-6588214 None:intergenic
TGGCATGGTAGAATGGAAAA+AGG 0.312351 5_2:+6587023 Msa0752870:intron
GACCTCATGCAATACTATTA+TGG 0.322975 5_2:+6587514 Msa0752870:three_prime_UTR
TTGAGTTTATTCCCGGATAA+TGG 0.328554 5_2:-6586380 None:intergenic
ATTTAACTGTTGCGGCAAAA+TGG 0.329724 5_2:+6586903 Msa0752870:CDS
TAGAAGAAAACATGACAATT+AGG 0.335380 5_2:+6587094 Msa0752870:CDS
TCCTCCTGTTATAGGATCAT+TGG 0.342440 5_2:-6587758 Msa0752880:CDS
TTGATGTTTGAGTTTATTCC+CGG 0.352803 5_2:-6586387 None:intergenic
GACTCACATTCAGCTGCATT+TGG 0.356576 5_2:-6587402 None:intergenic
GTTACCAAAACTATGCTTCC+AGG 0.364460 5_2:-6587226 None:intergenic
TTACAACACTTGAAAGAGTT+AGG 0.368447 5_2:-6587262 None:intergenic
CCCTCTCGTTGTTGGATACA+TGG 0.370228 5_2:-6587732 Msa0752880:CDS
CAGAGTTTCATGCCATTATC+CGG 0.370820 5_2:+6586368 Msa0752870:CDS
GGCCGGCTATAGGCGACTAT+TGG 0.377649 5_2:+6587790 Msa0752870:three_prime_UTR
AGCTATCTAGTAGTGGATTA+AGG 0.384056 5_2:-6587572 None:intergenic
AAAGGAAACAAAAGTACAAT+TGG 0.393314 5_2:-6587301 None:intergenic
ACCTTCATCAATCCATTTCA+AGG 0.398951 5_2:+6586687 Msa0752870:CDS
ATTGGTTGCCCTCTCGTTGT+TGG 0.399335 5_2:-6587740 Msa0752880:CDS
CAAACCTGGAAGCATAGTTT+TGG 0.405840 5_2:+6587222 Msa0752870:CDS
CAATCCATTTCAAGGAAAGA+AGG 0.408899 5_2:+6586695 Msa0752870:CDS
AAGTGGTGGAGGCCGGCTAT+AGG 0.418164 5_2:+6587780 Msa0752870:three_prime_UTR
TTCTTACAGCAGCTTACTGC+TGG 0.418688 5_2:+6586830 Msa0752870:CDS
AGGAACATGCATGTATGTTA+AGG 0.419513 5_2:-6587475 None:intergenic
TATAGTTACTCACCTGCAAT+TGG 0.426851 5_2:+6588180 Msa0752870:three_prime_UTR
AAAGATGAATCTGGCGAGAT+GGG 0.432844 5_2:-6587838 Msa0752880:CDS
CGGATAATGGCATGAAACTC+TGG 0.438289 5_2:-6586367 None:intergenic
GCAACCAATGATCCTATAAC+AGG 0.441435 5_2:+6587754 Msa0752870:three_prime_UTR
ACAGCAGCTTACTGCTGGCA+TGG 0.459854 5_2:+6586835 Msa0752870:CDS
GCCGGCTATAGGCGACTATT+GGG 0.460498 5_2:+6587791 Msa0752870:three_prime_UTR
ACATACATGCATGTTCCTGC+AGG 0.462917 5_2:+6587480 Msa0752870:three_prime_UTR
TCCTTGAAATGGATTGATGA+AGG 0.467392 5_2:-6586688 None:intergenic
ACCAATTGAATCAATCCAAA+AGG 0.487395 5_2:-6587319 None:intergenic
TACGTGTCAGTATCGTGTCT+TGG 0.489349 5_2:-6588271 None:intergenic
GATCCATTCACTTGGTAGAC+CGG 0.491216 5_2:+6588088 Msa0752870:three_prime_UTR
GGAACATGCATGTATGTTAA+GGG 0.494614 5_2:-6587474 None:intergenic
GAACAAATCCCATTCACAAC+AGG 0.495206 5_2:+6587045 Msa0752870:CDS
AAGTGATCTACTATCTCTGC+TGG 0.496660 5_2:-6586588 None:intergenic
AGCTGCAGCCTGTTGTGAAT+GGG 0.499013 5_2:-6587053 None:intergenic
AAGCTGCAGCCTGTTGTGAA+TGG 0.506105 5_2:-6587054 None:intergenic
AAGCCGGTCTACCAAGTGAA+TGG 0.508284 5_2:-6588091 Msa0752880:CDS
CTAAGACTATATTTAAGTAG+AGG 0.515504 5_2:+6587661 Msa0752870:three_prime_UTR
AATGGCATGAAACTCTGGTT+GGG 0.526617 5_2:-6586362 None:intergenic
AAAAGATGAATCTGGCGAGA+TGG 0.530203 5_2:-6587839 Msa0752880:CDS
GATCCTATAACAGGAGGAAG+TGG 0.536218 5_2:+6587763 Msa0752870:three_prime_UTR
CAGGAGGAAGTGGTGGAGGC+CGG 0.536906 5_2:+6587773 Msa0752870:three_prime_UTR
CAGCAGCTTACTGCTGGCAT+GGG 0.537521 5_2:+6586836 Msa0752870:CDS
TAATGGCATGAAACTCTGGT+TGG 0.544699 5_2:-6586363 None:intergenic
AGAGTTTCATGCCATTATCC+GGG 0.545323 5_2:+6586369 Msa0752870:CDS
ACCCAATAGTCGCCTATAGC+CGG 0.547078 5_2:-6587792 Msa0752880:CDS
GGGAGATGATTTAACTGTTG+CGG 0.550185 5_2:+6586895 Msa0752870:CDS
CTACCAAGTGAATGGATCAC+AGG 0.559874 5_2:-6588083 Msa0752880:CDS
AGAAACATTACAAATGGATG+TGG 0.563651 5_2:+6586329 None:intergenic
TAGATAGCTACATAAAGCTA+TGG 0.564545 5_2:+6587586 Msa0752870:three_prime_UTR
TGCTGTCCTCATCAGTGCTG+CGG 0.577568 5_2:+6587704 Msa0752870:three_prime_UTR
GTCATCAAACAATGAAGTCA+CGG 0.578442 5_2:-6587376 None:intergenic
ATAACAGGAGGAAGTGGTGG+AGG 0.586799 5_2:+6587769 Msa0752870:three_prime_UTR
ATTGAATCACTAGTCAAACC+TGG 0.588394 5_2:+6587208 Msa0752870:CDS
CCTATAACAGGAGGAAGTGG+TGG 0.596015 5_2:+6587766 Msa0752870:three_prime_UTR
GTGGAACCGCAGCACTGATG+AGG 0.597213 5_2:-6587710 Msa0752880:CDS
GTTGCGGCAAAATGGCACTT+AGG 0.602225 5_2:+6586911 Msa0752870:CDS
GGTCTTCTTAAGAACCCTGC+AGG 0.602800 5_2:-6587495 None:intergenic
TCTAGTAGTGGATTAAGGAA+AGG 0.610402 5_2:-6587567 None:intergenic
TTTATGTAGCTATCTAGTAG+TGG 0.616283 5_2:-6587579 None:intergenic
TACCCTGTGATCCATTCACT+TGG 0.621091 5_2:+6588080 Msa0752870:three_prime_UTR
TTTGCTAGGCGAAAAGCTGA+GGG 0.630682 5_2:-6586421 None:intergenic
CATACATGCATGTTCCTGCA+GGG 0.632925 5_2:+6587481 Msa0752870:three_prime_UTR
ATAGTCAGAAATATATGCGA+GGG 0.636276 5_2:+6586875 Msa0752870:CDS
AAGATGAATCTGGCGAGATG+GGG 0.637726 5_2:-6587837 Msa0752880:CDS
ATCCATAATAGTATTGCATG+AGG 0.639529 5_2:-6587516 None:intergenic
CATAGTCAGAAATATATGCG+AGG 0.642978 5_2:+6586874 Msa0752870:CDS
ACCAATGATCCTATAACAGG+AGG 0.648824 5_2:+6587757 Msa0752870:three_prime_UTR
ACCATGTATCCAACAACGAG+AGG 0.650347 5_2:+6587731 Msa0752870:three_prime_UTR
CCATGTATCCAACAACGAGA+GGG 0.655825 5_2:+6587732 Msa0752870:three_prime_UTR
AGCTGAATGTGAGTCAAACA+TGG 0.656790 5_2:+6587410 Msa0752870:three_prime_UTR
CTATAGATAAAGTTCATGCG+TGG 0.662656 5_2:-6588154 None:intergenic
TACCAAGTGAATGGATCACA+GGG 0.666568 5_2:-6588082 Msa0752880:intron
TCTCGTTGTTGGATACATGG+TGG 0.695822 5_2:-6587729 Msa0752880:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTAAATTTTTCCCGAATTT+GGG - chr5_2:6586564-6586583 None:intergenic 20.0%
!! GCTTATATCATTTATACTTT+AGG - chr5_2:6586987-6587006 None:intergenic 20.0%
!!! TTTCTTCTACTTTTTCTAAT+TGG - chr5_2:6587083-6587102 None:intergenic 20.0%
!!! ATGATATTTTGCATTACTAT+AGG - chr5_2:6587889-6587908 Msa0752880:intron 20.0%
!!! TTTAAATTTTTCCCGAATTT+GGG - chr5_2:6586564-6586583 None:intergenic 20.0%
!! GCTTATATCATTTATACTTT+AGG - chr5_2:6586987-6587006 None:intergenic 20.0%
!!! TTTCTTCTACTTTTTCTAAT+TGG - chr5_2:6587083-6587102 None:intergenic 20.0%
!!! ATGATATTTTGCATTACTAT+AGG - chr5_2:6587889-6587908 Msa0752880:intron 20.0%
! TCAGTCATATTATCAAAAAG+AGG + chr5_2:6586537-6586556 Msa0752870:CDS 25.0%
!! TTAAATTTTTCCCGAATTTG+GGG - chr5_2:6586563-6586582 None:intergenic 25.0%
!! GTTTAAATTTTTCCCGAATT+TGG - chr5_2:6586565-6586584 None:intergenic 25.0%
! TGAAAAAGAGCTAAAACAAT+TGG + chr5_2:6586626-6586645 Msa0752870:CDS 25.0%
! TAATCTAAACATAACATTGC+AGG + chr5_2:6586796-6586815 Msa0752870:intron 25.0%
! AAAAGCAATAAATCTAGGAA+AGG - chr5_2:6586944-6586963 None:intergenic 25.0%
! TAGAAGAAAACATGACAATT+AGG + chr5_2:6587094-6587113 Msa0752870:CDS 25.0%
! CTCACTATATAATTGAATTC+AGG + chr5_2:6587170-6587189 Msa0752870:intron 25.0%
!! AAAGGAAACAAAAGTACAAT+TGG - chr5_2:6587304-6587323 None:intergenic 25.0%
!!! ATTGTACTTTTGTTTCCTTT+TGG + chr5_2:6587304-6587323 Msa0752870:three_prime_UTR 25.0%
!! GTATGTTAAGGGATATTTTT+GGG - chr5_2:6587466-6587485 None:intergenic 25.0%
!! TGTATGTTAAGGGATATTTT+TGG - chr5_2:6587467-6587486 None:intergenic 25.0%
! CTAAGACTATATTTAAGTAG+AGG + chr5_2:6587661-6587680 Msa0752870:three_prime_UTR 25.0%
! AAACAAGAAAAAGATGAATC+TGG - chr5_2:6587850-6587869 Msa0752880:CDS 25.0%
!!! TCATCTTTTTCTTGTTTCTT+AGG + chr5_2:6587853-6587872 Msa0752870:three_prime_UTR 25.0%
! TTGCATTACTATAGGTATAT+TGG - chr5_2:6587881-6587900 Msa0752880:intron 25.0%
! TCAGTCATATTATCAAAAAG+AGG + chr5_2:6586537-6586556 Msa0752870:CDS 25.0%
!! TTAAATTTTTCCCGAATTTG+GGG - chr5_2:6586563-6586582 None:intergenic 25.0%
!! GTTTAAATTTTTCCCGAATT+TGG - chr5_2:6586565-6586584 None:intergenic 25.0%
! TGAAAAAGAGCTAAAACAAT+TGG + chr5_2:6586626-6586645 Msa0752870:CDS 25.0%
! TAATCTAAACATAACATTGC+AGG + chr5_2:6586796-6586815 Msa0752870:intron 25.0%
! AAAAGCAATAAATCTAGGAA+AGG - chr5_2:6586944-6586963 None:intergenic 25.0%
! TAGAAGAAAACATGACAATT+AGG + chr5_2:6587094-6587113 Msa0752870:CDS 25.0%
! CTCACTATATAATTGAATTC+AGG + chr5_2:6587170-6587189 Msa0752870:intron 25.0%
!! AAAGGAAACAAAAGTACAAT+TGG - chr5_2:6587304-6587323 None:intergenic 25.0%
!!! ATTGTACTTTTGTTTCCTTT+TGG + chr5_2:6587304-6587323 Msa0752870:three_prime_UTR 25.0%
!! GTATGTTAAGGGATATTTTT+GGG - chr5_2:6587466-6587485 None:intergenic 25.0%
!! TGTATGTTAAGGGATATTTT+TGG - chr5_2:6587467-6587486 None:intergenic 25.0%
! CTAAGACTATATTTAAGTAG+AGG + chr5_2:6587661-6587680 Msa0752870:three_prime_UTR 25.0%
! AAACAAGAAAAAGATGAATC+TGG - chr5_2:6587850-6587869 Msa0752880:CDS 25.0%
!!! TCATCTTTTTCTTGTTTCTT+AGG + chr5_2:6587853-6587872 Msa0752870:three_prime_UTR 25.0%
! TTGCATTACTATAGGTATAT+TGG - chr5_2:6587881-6587900 Msa0752880:intron 25.0%
! TTGATGTTTGAGTTTATTCC+CGG - chr5_2:6586390-6586409 None:intergenic 30.0%
AGAATGTCAAAAACAGAACT+TGG + chr5_2:6586499-6586518 Msa0752870:CDS 30.0%
GAATGTCAAAAACAGAACTT+GGG + chr5_2:6586500-6586519 Msa0752870:CDS 30.0%
GTTATTAGAACTTAGAAGGA+TGG - chr5_2:6586733-6586752 None:intergenic 30.0%
CTAGGTTATTAGAACTTAGA+AGG - chr5_2:6586737-6586756 None:intergenic 30.0%
TGACTATGAATTTCACATTC+TGG - chr5_2:6586862-6586881 None:intergenic 30.0%
ATAGTCAGAAATATATGCGA+GGG + chr5_2:6586875-6586894 Msa0752870:CDS 30.0%
AGCAGAAAAGCAATAAATCT+AGG - chr5_2:6586949-6586968 None:intergenic 30.0%
! AAAGAGTTAGGTTCTGTTTT+AGG - chr5_2:6587253-6587272 None:intergenic 30.0%
TTACAACACTTGAAAGAGTT+AGG - chr5_2:6587265-6587284 None:intergenic 30.0%
!! TCCTTTTGGATTGATTCAAT+TGG + chr5_2:6587318-6587337 Msa0752870:three_prime_UTR 30.0%
ACCAATTGAATCAATCCAAA+AGG - chr5_2:6587322-6587341 None:intergenic 30.0%
ATCCATAATAGTATTGCATG+AGG - chr5_2:6587519-6587538 None:intergenic 30.0%
TTTATGTAGCTATCTAGTAG+TGG - chr5_2:6587582-6587601 None:intergenic 30.0%
TAGATAGCTACATAAAGCTA+TGG + chr5_2:6587586-6587605 Msa0752870:three_prime_UTR 30.0%
!! TAGTTTGAGTGTTGAACAAT+GGG - chr5_2:6587972-6587991 Msa0752880:intron 30.0%
AAGTACAAGCATGAAAAACT+CGG + chr5_2:6588225-6588244 Msa0752870:three_prime_UTR 30.0%
! TTGATGTTTGAGTTTATTCC+CGG - chr5_2:6586390-6586409 None:intergenic 30.0%
AGAATGTCAAAAACAGAACT+TGG + chr5_2:6586499-6586518 Msa0752870:CDS 30.0%
GAATGTCAAAAACAGAACTT+GGG + chr5_2:6586500-6586519 Msa0752870:CDS 30.0%
GTTATTAGAACTTAGAAGGA+TGG - chr5_2:6586733-6586752 None:intergenic 30.0%
CTAGGTTATTAGAACTTAGA+AGG - chr5_2:6586737-6586756 None:intergenic 30.0%
TGACTATGAATTTCACATTC+TGG - chr5_2:6586862-6586881 None:intergenic 30.0%
ATAGTCAGAAATATATGCGA+GGG + chr5_2:6586875-6586894 Msa0752870:CDS 30.0%
AGCAGAAAAGCAATAAATCT+AGG - chr5_2:6586949-6586968 None:intergenic 30.0%
! AAAGAGTTAGGTTCTGTTTT+AGG - chr5_2:6587253-6587272 None:intergenic 30.0%
TTACAACACTTGAAAGAGTT+AGG - chr5_2:6587265-6587284 None:intergenic 30.0%
!! TCCTTTTGGATTGATTCAAT+TGG + chr5_2:6587318-6587337 Msa0752870:three_prime_UTR 30.0%
ACCAATTGAATCAATCCAAA+AGG - chr5_2:6587322-6587341 None:intergenic 30.0%
ATCCATAATAGTATTGCATG+AGG - chr5_2:6587519-6587538 None:intergenic 30.0%
TTTATGTAGCTATCTAGTAG+TGG - chr5_2:6587582-6587601 None:intergenic 30.0%
TAGATAGCTACATAAAGCTA+TGG + chr5_2:6587586-6587605 Msa0752870:three_prime_UTR 30.0%
!! TAGTTTGAGTGTTGAACAAT+GGG - chr5_2:6587972-6587991 Msa0752880:intron 30.0%
AAGTACAAGCATGAAAAACT+CGG + chr5_2:6588225-6588244 Msa0752870:three_prime_UTR 30.0%
TTGAGTTTATTCCCGGATAA+TGG - chr5_2:6586383-6586402 None:intergenic 35.0%
! AAGTTGCATGATGTTTTGCT+AGG - chr5_2:6586438-6586457 None:intergenic 35.0%
ATGCAACTTGCACAAAAAGA+TGG + chr5_2:6586449-6586468 Msa0752870:CDS 35.0%
ACCTTCATCAATCCATTTCA+AGG + chr5_2:6586687-6586706 Msa0752870:CDS 35.0%
!! TCCTTGAAATGGATTGATGA+AGG - chr5_2:6586691-6586710 None:intergenic 35.0%
CAATCCATTTCAAGGAAAGA+AGG + chr5_2:6586695-6586714 Msa0752870:CDS 35.0%
CAAACCTTCTTTCCTTGAAA+TGG - chr5_2:6586702-6586721 None:intergenic 35.0%
AGCTGTTTACTCGATTCAAT+CGG - chr5_2:6586768-6586787 None:intergenic 35.0%
CATAGTCAGAAATATATGCG+AGG + chr5_2:6586874-6586893 Msa0752870:CDS 35.0%
ATTTAACTGTTGCGGCAAAA+TGG + chr5_2:6586903-6586922 Msa0752870:CDS 35.0%
! AAGCCTGACGAATATCTTTT+TGG + chr5_2:6587003-6587022 Msa0752870:intron 35.0%
ATGCCAAAAAGATATTCGTC+AGG - chr5_2:6587009-6587028 None:intergenic 35.0%
!! TGACGAATATCTTTTTGGCA+TGG + chr5_2:6587008-6587027 Msa0752870:intron 35.0%
!! ATCTTTTTGGCATGGTAGAA+TGG + chr5_2:6587016-6587035 Msa0752870:intron 35.0%
ATTGAATCACTAGTCAAACC+TGG + chr5_2:6587208-6587227 Msa0752870:CDS 35.0%
GTCATCAAACAATGAAGTCA+CGG - chr5_2:6587379-6587398 None:intergenic 35.0%
GGAACATGCATGTATGTTAA+GGG - chr5_2:6587477-6587496 None:intergenic 35.0%
AGGAACATGCATGTATGTTA+AGG - chr5_2:6587478-6587497 None:intergenic 35.0%
GACCTCATGCAATACTATTA+TGG + chr5_2:6587514-6587533 Msa0752870:three_prime_UTR 35.0%
!! TCTAGTAGTGGATTAAGGAA+AGG - chr5_2:6587570-6587589 None:intergenic 35.0%
!! AGCTATCTAGTAGTGGATTA+AGG - chr5_2:6587575-6587594 None:intergenic 35.0%
!! CTAGTTTGAGTGTTGAACAA+TGG - chr5_2:6587973-6587992 Msa0752880:intron 35.0%
TGCGAAAAACAGATTTAAGC+CGG - chr5_2:6588110-6588129 Msa0752880:CDS 35.0%
CTATAGATAAAGTTCATGCG+TGG - chr5_2:6588157-6588176 None:intergenic 35.0%
TATAGTTACTCACCTGCAAT+TGG + chr5_2:6588180-6588199 Msa0752870:three_prime_UTR 35.0%
!!! GCTTGTTTTATTCCAATTGC+AGG - chr5_2:6588195-6588214 None:intergenic 35.0%
ATGCTTGTACTTCAGATCTA+AGG - chr5_2:6588217-6588236 None:intergenic 35.0%
TTGAGTTTATTCCCGGATAA+TGG - chr5_2:6586383-6586402 None:intergenic 35.0%
! AAGTTGCATGATGTTTTGCT+AGG - chr5_2:6586438-6586457 None:intergenic 35.0%
ATGCAACTTGCACAAAAAGA+TGG + chr5_2:6586449-6586468 Msa0752870:CDS 35.0%
ACCTTCATCAATCCATTTCA+AGG + chr5_2:6586687-6586706 Msa0752870:CDS 35.0%
!! TCCTTGAAATGGATTGATGA+AGG - chr5_2:6586691-6586710 None:intergenic 35.0%
CAATCCATTTCAAGGAAAGA+AGG + chr5_2:6586695-6586714 Msa0752870:CDS 35.0%
CAAACCTTCTTTCCTTGAAA+TGG - chr5_2:6586702-6586721 None:intergenic 35.0%
AGCTGTTTACTCGATTCAAT+CGG - chr5_2:6586768-6586787 None:intergenic 35.0%
CATAGTCAGAAATATATGCG+AGG + chr5_2:6586874-6586893 Msa0752870:CDS 35.0%
ATTTAACTGTTGCGGCAAAA+TGG + chr5_2:6586903-6586922 Msa0752870:CDS 35.0%
! AAGCCTGACGAATATCTTTT+TGG + chr5_2:6587003-6587022 Msa0752870:intron 35.0%
ATGCCAAAAAGATATTCGTC+AGG - chr5_2:6587009-6587028 None:intergenic 35.0%
!! TGACGAATATCTTTTTGGCA+TGG + chr5_2:6587008-6587027 Msa0752870:intron 35.0%
!! ATCTTTTTGGCATGGTAGAA+TGG + chr5_2:6587016-6587035 Msa0752870:intron 35.0%
ATTGAATCACTAGTCAAACC+TGG + chr5_2:6587208-6587227 Msa0752870:CDS 35.0%
GTCATCAAACAATGAAGTCA+CGG - chr5_2:6587379-6587398 None:intergenic 35.0%
GGAACATGCATGTATGTTAA+GGG - chr5_2:6587477-6587496 None:intergenic 35.0%
AGGAACATGCATGTATGTTA+AGG - chr5_2:6587478-6587497 None:intergenic 35.0%
GACCTCATGCAATACTATTA+TGG + chr5_2:6587514-6587533 Msa0752870:three_prime_UTR 35.0%
!! TCTAGTAGTGGATTAAGGAA+AGG - chr5_2:6587570-6587589 None:intergenic 35.0%
!! AGCTATCTAGTAGTGGATTA+AGG - chr5_2:6587575-6587594 None:intergenic 35.0%
!! CTAGTTTGAGTGTTGAACAA+TGG - chr5_2:6587973-6587992 Msa0752880:intron 35.0%
TGCGAAAAACAGATTTAAGC+CGG - chr5_2:6588110-6588129 Msa0752880:CDS 35.0%
CTATAGATAAAGTTCATGCG+TGG - chr5_2:6588157-6588176 None:intergenic 35.0%
TATAGTTACTCACCTGCAAT+TGG + chr5_2:6588180-6588199 Msa0752870:three_prime_UTR 35.0%
!!! GCTTGTTTTATTCCAATTGC+AGG - chr5_2:6588195-6588214 None:intergenic 35.0%
ATGCTTGTACTTCAGATCTA+AGG - chr5_2:6588217-6588236 None:intergenic 35.0%
TGAAACTCTGGTTGGGAATT+TGG - chr5_2:6586358-6586377 None:intergenic 40.0%
AATGGCATGAAACTCTGGTT+GGG - chr5_2:6586365-6586384 None:intergenic 40.0%
TAATGGCATGAAACTCTGGT+TGG - chr5_2:6586366-6586385 None:intergenic 40.0%
CAGAGTTTCATGCCATTATC+CGG + chr5_2:6586368-6586387 Msa0752870:CDS 40.0%
AGAGTTTCATGCCATTATCC+GGG + chr5_2:6586369-6586388 Msa0752870:CDS 40.0%
TCAAAAAGAGGCCCCAAATT+CGG + chr5_2:6586549-6586568 Msa0752870:CDS 40.0%
AAGTGATCTACTATCTCTGC+TGG - chr5_2:6586591-6586610 None:intergenic 40.0%
ATTCAATCGGGGTTACTTCT+AGG - chr5_2:6586755-6586774 None:intergenic 40.0%
CTGTTTACTCGATTCAATCG+GGG - chr5_2:6586766-6586785 None:intergenic 40.0%
GCTGTTTACTCGATTCAATC+GGG - chr5_2:6586767-6586786 None:intergenic 40.0%
GGGAGATGATTTAACTGTTG+CGG + chr5_2:6586895-6586914 Msa0752870:CDS 40.0%
TGGCATGGTAGAATGGAAAA+AGG + chr5_2:6587023-6587042 Msa0752870:intron 40.0%
GAACAAATCCCATTCACAAC+AGG + chr5_2:6587045-6587064 Msa0752870:CDS 40.0%
!! CAAACCTGGAAGCATAGTTT+TGG + chr5_2:6587222-6587241 Msa0752870:CDS 40.0%
GTTACCAAAACTATGCTTCC+AGG - chr5_2:6587229-6587248 None:intergenic 40.0%
AGCTGAATGTGAGTCAAACA+TGG + chr5_2:6587410-6587429 Msa0752870:three_prime_UTR 40.0%
GCAACCAATGATCCTATAAC+AGG + chr5_2:6587754-6587773 Msa0752870:three_prime_UTR 40.0%
ACCAATGATCCTATAACAGG+AGG + chr5_2:6587757-6587776 Msa0752870:three_prime_UTR 40.0%
TCCTCCTGTTATAGGATCAT+TGG - chr5_2:6587761-6587780 Msa0752880:CDS 40.0%
AAAGATGAATCTGGCGAGAT+GGG - chr5_2:6587841-6587860 Msa0752880:CDS 40.0%
AAAAGATGAATCTGGCGAGA+TGG - chr5_2:6587842-6587861 Msa0752880:CDS 40.0%
TACCAAGTGAATGGATCACA+GGG - chr5_2:6588085-6588104 Msa0752880:CDS 40.0%
TGAAACTCTGGTTGGGAATT+TGG - chr5_2:6586358-6586377 None:intergenic 40.0%
AATGGCATGAAACTCTGGTT+GGG - chr5_2:6586365-6586384 None:intergenic 40.0%
TAATGGCATGAAACTCTGGT+TGG - chr5_2:6586366-6586385 None:intergenic 40.0%
CAGAGTTTCATGCCATTATC+CGG + chr5_2:6586368-6586387 Msa0752870:CDS 40.0%
AGAGTTTCATGCCATTATCC+GGG + chr5_2:6586369-6586388 Msa0752870:CDS 40.0%
TCAAAAAGAGGCCCCAAATT+CGG + chr5_2:6586549-6586568 Msa0752870:CDS 40.0%
AAGTGATCTACTATCTCTGC+TGG - chr5_2:6586591-6586610 None:intergenic 40.0%
ATTCAATCGGGGTTACTTCT+AGG - chr5_2:6586755-6586774 None:intergenic 40.0%
CTGTTTACTCGATTCAATCG+GGG - chr5_2:6586766-6586785 None:intergenic 40.0%
GCTGTTTACTCGATTCAATC+GGG - chr5_2:6586767-6586786 None:intergenic 40.0%
GGGAGATGATTTAACTGTTG+CGG + chr5_2:6586895-6586914 Msa0752870:CDS 40.0%
TGGCATGGTAGAATGGAAAA+AGG + chr5_2:6587023-6587042 Msa0752870:intron 40.0%
GAACAAATCCCATTCACAAC+AGG + chr5_2:6587045-6587064 Msa0752870:CDS 40.0%
!! CAAACCTGGAAGCATAGTTT+TGG + chr5_2:6587222-6587241 Msa0752870:CDS 40.0%
GTTACCAAAACTATGCTTCC+AGG - chr5_2:6587229-6587248 None:intergenic 40.0%
AGCTGAATGTGAGTCAAACA+TGG + chr5_2:6587410-6587429 Msa0752870:three_prime_UTR 40.0%
GCAACCAATGATCCTATAAC+AGG + chr5_2:6587754-6587773 Msa0752870:three_prime_UTR 40.0%
ACCAATGATCCTATAACAGG+AGG + chr5_2:6587757-6587776 Msa0752870:three_prime_UTR 40.0%
TCCTCCTGTTATAGGATCAT+TGG - chr5_2:6587761-6587780 Msa0752880:CDS 40.0%
AAAGATGAATCTGGCGAGAT+GGG - chr5_2:6587841-6587860 Msa0752880:CDS 40.0%
AAAAGATGAATCTGGCGAGA+TGG - chr5_2:6587842-6587861 Msa0752880:CDS 40.0%
TACCAAGTGAATGGATCACA+GGG - chr5_2:6588085-6588104 Msa0752880:CDS 40.0%
! CGGATAATGGCATGAAACTC+TGG - chr5_2:6586370-6586389 None:intergenic 45.0%
TTTGCTAGGCGAAAAGCTGA+GGG - chr5_2:6586424-6586443 None:intergenic 45.0%
! TTTTGCTAGGCGAAAAGCTG+AGG - chr5_2:6586425-6586444 None:intergenic 45.0%
CAAAAAGAGGCCCCAAATTC+GGG + chr5_2:6586550-6586569 Msa0752870:CDS 45.0%
TTCTTACAGCAGCTTACTGC+TGG + chr5_2:6586830-6586849 Msa0752870:CDS 45.0%
GACTCACATTCAGCTGCATT+TGG - chr5_2:6587405-6587424 None:intergenic 45.0%
ACATACATGCATGTTCCTGC+AGG + chr5_2:6587480-6587499 Msa0752870:three_prime_UTR 45.0%
CATACATGCATGTTCCTGCA+GGG + chr5_2:6587481-6587500 Msa0752870:three_prime_UTR 45.0%
TCTCGTTGTTGGATACATGG+TGG - chr5_2:6587732-6587751 Msa0752880:CDS 45.0%
ACCATGTATCCAACAACGAG+AGG + chr5_2:6587731-6587750 Msa0752870:three_prime_UTR 45.0%
CCATGTATCCAACAACGAGA+GGG + chr5_2:6587732-6587751 Msa0752870:three_prime_UTR 45.0%
GATCCTATAACAGGAGGAAG+TGG + chr5_2:6587763-6587782 Msa0752870:three_prime_UTR 45.0%
AAGATGAATCTGGCGAGATG+GGG - chr5_2:6587840-6587859 Msa0752880:CDS 45.0%
TACCCTGTGATCCATTCACT+TGG + chr5_2:6588080-6588099 Msa0752870:three_prime_UTR 45.0%
CTACCAAGTGAATGGATCAC+AGG - chr5_2:6588086-6588105 Msa0752880:CDS 45.0%
GATCCATTCACTTGGTAGAC+CGG + chr5_2:6588088-6588107 Msa0752870:three_prime_UTR 45.0%
TACGTGTCAGTATCGTGTCT+TGG - chr5_2:6588274-6588293 None:intergenic 45.0%
! CGGATAATGGCATGAAACTC+TGG - chr5_2:6586370-6586389 None:intergenic 45.0%
TTTGCTAGGCGAAAAGCTGA+GGG - chr5_2:6586424-6586443 None:intergenic 45.0%
! TTTTGCTAGGCGAAAAGCTG+AGG - chr5_2:6586425-6586444 None:intergenic 45.0%
CAAAAAGAGGCCCCAAATTC+GGG + chr5_2:6586550-6586569 Msa0752870:CDS 45.0%
TTCTTACAGCAGCTTACTGC+TGG + chr5_2:6586830-6586849 Msa0752870:CDS 45.0%
GACTCACATTCAGCTGCATT+TGG - chr5_2:6587405-6587424 None:intergenic 45.0%
ACATACATGCATGTTCCTGC+AGG + chr5_2:6587480-6587499 Msa0752870:three_prime_UTR 45.0%
CATACATGCATGTTCCTGCA+GGG + chr5_2:6587481-6587500 Msa0752870:three_prime_UTR 45.0%
TCTCGTTGTTGGATACATGG+TGG - chr5_2:6587732-6587751 Msa0752880:CDS 45.0%
ACCATGTATCCAACAACGAG+AGG + chr5_2:6587731-6587750 Msa0752870:three_prime_UTR 45.0%
CCATGTATCCAACAACGAGA+GGG + chr5_2:6587732-6587751 Msa0752870:three_prime_UTR 45.0%
GATCCTATAACAGGAGGAAG+TGG + chr5_2:6587763-6587782 Msa0752870:three_prime_UTR 45.0%
AAGATGAATCTGGCGAGATG+GGG - chr5_2:6587840-6587859 Msa0752880:CDS 45.0%
TACCCTGTGATCCATTCACT+TGG + chr5_2:6588080-6588099 Msa0752870:three_prime_UTR 45.0%
CTACCAAGTGAATGGATCAC+AGG - chr5_2:6588086-6588105 Msa0752880:CDS 45.0%
GATCCATTCACTTGGTAGAC+CGG + chr5_2:6588088-6588107 Msa0752870:three_prime_UTR 45.0%
TACGTGTCAGTATCGTGTCT+TGG - chr5_2:6588274-6588293 None:intergenic 45.0%
GTTGCGGCAAAATGGCACTT+AGG + chr5_2:6586911-6586930 Msa0752870:CDS 50.0%
AGCTGCAGCCTGTTGTGAAT+GGG - chr5_2:6587056-6587075 None:intergenic 50.0%
AAGCTGCAGCCTGTTGTGAA+TGG - chr5_2:6587057-6587076 None:intergenic 50.0%
GGTCTTCTTAAGAACCCTGC+AGG - chr5_2:6587498-6587517 None:intergenic 50.0%
CCCTCTCGTTGTTGGATACA+TGG - chr5_2:6587735-6587754 Msa0752880:CDS 50.0%
ATTGGTTGCCCTCTCGTTGT+TGG - chr5_2:6587743-6587762 Msa0752880:CDS 50.0%
CCACCACTTCCTCCTGTTAT+AGG - chr5_2:6587769-6587788 Msa0752880:CDS 50.0%
CCTATAACAGGAGGAAGTGG+TGG + chr5_2:6587766-6587785 Msa0752870:three_prime_UTR 50.0%
ATAACAGGAGGAAGTGGTGG+AGG + chr5_2:6587769-6587788 Msa0752870:three_prime_UTR 50.0%
ACCCAATAGTCGCCTATAGC+CGG - chr5_2:6587795-6587814 Msa0752880:CDS 50.0%
AAGCCGGTCTACCAAGTGAA+TGG - chr5_2:6588094-6588113 Msa0752880:CDS 50.0%
GTTGCGGCAAAATGGCACTT+AGG + chr5_2:6586911-6586930 Msa0752870:CDS 50.0%
AGCTGCAGCCTGTTGTGAAT+GGG - chr5_2:6587056-6587075 None:intergenic 50.0%
AAGCTGCAGCCTGTTGTGAA+TGG - chr5_2:6587057-6587076 None:intergenic 50.0%
GGTCTTCTTAAGAACCCTGC+AGG - chr5_2:6587498-6587517 None:intergenic 50.0%
CCCTCTCGTTGTTGGATACA+TGG - chr5_2:6587735-6587754 Msa0752880:CDS 50.0%
ATTGGTTGCCCTCTCGTTGT+TGG - chr5_2:6587743-6587762 Msa0752880:CDS 50.0%
CCACCACTTCCTCCTGTTAT+AGG - chr5_2:6587769-6587788 Msa0752880:CDS 50.0%
CCTATAACAGGAGGAAGTGG+TGG + chr5_2:6587766-6587785 Msa0752870:three_prime_UTR 50.0%
ATAACAGGAGGAAGTGGTGG+AGG + chr5_2:6587769-6587788 Msa0752870:three_prime_UTR 50.0%
ACCCAATAGTCGCCTATAGC+CGG - chr5_2:6587795-6587814 Msa0752880:CDS 50.0%
AAGCCGGTCTACCAAGTGAA+TGG - chr5_2:6588094-6588113 Msa0752880:CDS 50.0%
ACAGCAGCTTACTGCTGGCA+TGG + chr5_2:6586835-6586854 Msa0752870:CDS 55.0%
CAGCAGCTTACTGCTGGCAT+GGG + chr5_2:6586836-6586855 Msa0752870:CDS 55.0%
TGCTGTCCTCATCAGTGCTG+CGG + chr5_2:6587704-6587723 Msa0752870:three_prime_UTR 55.0%
GCCGGCTATAGGCGACTATT+GGG + chr5_2:6587791-6587810 Msa0752870:three_prime_UTR 55.0%
ACAGCAGCTTACTGCTGGCA+TGG + chr5_2:6586835-6586854 Msa0752870:CDS 55.0%
CAGCAGCTTACTGCTGGCAT+GGG + chr5_2:6586836-6586855 Msa0752870:CDS 55.0%
TGCTGTCCTCATCAGTGCTG+CGG + chr5_2:6587704-6587723 Msa0752870:three_prime_UTR 55.0%
GCCGGCTATAGGCGACTATT+GGG + chr5_2:6587791-6587810 Msa0752870:three_prime_UTR 55.0%
!! GTGGAACCGCAGCACTGATG+AGG - chr5_2:6587713-6587732 Msa0752880:CDS 60.0%
! AAGTGGTGGAGGCCGGCTAT+AGG + chr5_2:6587780-6587799 Msa0752870:three_prime_UTR 60.0%
GGCCGGCTATAGGCGACTAT+TGG + chr5_2:6587790-6587809 Msa0752870:three_prime_UTR 60.0%
!! GTGGAACCGCAGCACTGATG+AGG - chr5_2:6587713-6587732 Msa0752880:CDS 60.0%
! AAGTGGTGGAGGCCGGCTAT+AGG + chr5_2:6587780-6587799 Msa0752870:three_prime_UTR 60.0%
GGCCGGCTATAGGCGACTAT+TGG + chr5_2:6587790-6587809 Msa0752870:three_prime_UTR 60.0%
! CAGGAGGAAGTGGTGGAGGC+CGG + chr5_2:6587773-6587792 Msa0752870:three_prime_UTR 65.0%
! CAGGAGGAAGTGGTGGAGGC+CGG + chr5_2:6587773-6587792 Msa0752870:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 6586342 6588312 6586342 ID=Msa0752870;Name=Msa0752870
chr5_2 mRNA 6586342 6588312 6586342 ID=Msa0752870-mRNA-1;Parent=Msa0752870;Name=Msa0752870-mRNA-1;_AED=0.08;_eAED=0.08;_QI=0|1|0.8|1|0.75|0.6|5|819|225
chr5_2 exon 6586342 6586716 6586342 ID=Msa0752870-mRNA-1:exon:2752;Parent=Msa0752870-mRNA-1
chr5_2 exon 6586818 6586932 6586818 ID=Msa0752870-mRNA-1:exon:2753;Parent=Msa0752870-mRNA-1
chr5_2 exon 6587034 6587115 6587034 ID=Msa0752870-mRNA-1:exon:2754;Parent=Msa0752870-mRNA-1
chr5_2 exon 6587192 6587882 6587192 ID=Msa0752870-mRNA-1:exon:2755;Parent=Msa0752870-mRNA-1
chr5_2 exon 6588079 6588312 6588079 ID=Msa0752870-mRNA-1:exon:2756;Parent=Msa0752870-mRNA-1
chr5_2 CDS 6586342 6586716 6586342 ID=Msa0752870-mRNA-1:cds;Parent=Msa0752870-mRNA-1
chr5_2 CDS 6586818 6586932 6586818 ID=Msa0752870-mRNA-1:cds;Parent=Msa0752870-mRNA-1
chr5_2 CDS 6587034 6587115 6587034 ID=Msa0752870-mRNA-1:cds;Parent=Msa0752870-mRNA-1
chr5_2 CDS 6587192 6587297 6587192 ID=Msa0752870-mRNA-1:cds;Parent=Msa0752870-mRNA-1
chr5_2 three_prime_UTR 6587298 6587882 6587298 ID=Msa0752870-mRNA-1:three_prime_utr;Parent=Msa0752870-mRNA-1
chr5_2 three_prime_UTR 6588079 6588312 6588079 ID=Msa0752870-mRNA-1:three_prime_utr;Parent=Msa0752870-mRNA-1
Gene Sequence

>Msa0752870

ATGGATGTGGTAGCCAAATTCCCAACCAGAGTTTCATGCCATTATCCGGGAATAAACTCAAACATCAAAAAGAGACTCGCCCTCAGCTTTTCGCCTAGCAAAACATCATGCAACTTGCACAAAAAGATGGAGATACAACAAAGAAGCATTTGTTTCAAGAATGTCAAAAACAGAACTTGGGATAACAATATAATTTCAGTCATATTATCAAAAAGAGGCCCCAAATTCGGGAAAAATTTAAACTCTCCAGCAGAGATAGTAGATCACTTCTACACATGTATCAATGAAAAAGAGCTAAAACAATTGGATGAATACATATCACAAGATGCTTGCTTTTATGACTATACCTTCATCAATCCATTTCAAGGAAAGAAGGAAGTGATGCATTTCTTACAGCAGCTTACTGCTGGCATGGGCCAGAATGTGAAATTCATAGTCAGAAATATATGCGAGGGAGATGATTTAACTGTTGCGGCAAAATGGCACTTAGAATGGAAAAAGGAACAAATCCCATTCACAACAGGCTGCAGCTTCTTCCAATTAGAAAAAGTAGAAGAAAACATGACAATTAGGAGAGCTGAGATATTTATTGAATCACTAGTCAAACCTGGAAGCATAGTTTTGGTAACTCCTAAAACAGAACCTAACTCTTTCAAGTGTTGTAATTCTTATACGTGA
Protein sequence

>Msa0752870

MDVVAKFPTRVSCHYPGINSNIKKRLALSFSPSKTSCNLHKKMEIQQRSICFKNVKNRTWDNNIISVILSKRGPKFGKNLNSPAEIVDHFYTCINEKELKQLDEYISQDACFYDYTFINPFQGKKEVMHFLQQLTAGMGQNVKFIVRNICEGDDLTVAAKWHLEWKKEQIPFTTGCSFFQLEKVEENMTIRRAEIFIESLVKPGSIVLVTPKTEPNSFKCCNSYT*