AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1356760

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1356760 MtrunA17_Chr5g0397281 90.367 218 21 0 1 218 1 218 1.04e-146 410
Msa1356760 MtrunA17_Chr3g0126191 33.824 136 90 0 73 208 76 211 2.46e-24 97.1
Msa1356760 MtrunA17_Chr7g0221181 30.827 133 92 0 81 213 76 208 2.11e-23 94.0
Msa1356760 MtrunA17_Chr2g0289851 23.762 202 134 6 19 201 13 213 3.22e-12 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1356760 AT5G41470.1 48.148 135 70 0 74 208 66 200 8.99e-38 133
Msa1356760 AT1G71480.1 32.576 132 89 0 81 212 69 200 9.00e-26 100
Msa1356760 AT1G71480.3 33.333 105 70 0 81 185 69 173 5.26e-23 92.0
Msa1356760 AT1G71480.2 28.916 83 59 0 81 163 69 151 2.31e-12 63.2

Find 81 sgRNAs with CRISPR-Local

Find 248 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAAACTCTGGTTGGGAATT+TGG 0.130689 tig0015859:-29457 Msa1356750:intron
CCACCACTTCCTCCTGTTAT+AGG 0.246729 tig0015859:-30864 Msa1356770:CDS
TGACTATGAATTTCACATTC+TGG 0.276043 tig0015859:-29961 Msa1356750:intron
CAAACCTTCTTTCCTTGAAA+TGG 0.293968 tig0015859:-29801 Msa1356750:intron
TGGCATGGTAGAATGGAAAA+AGG 0.312351 tig0015859:+30125 Msa1356760:intron
TAGAAGAAAACATGACAATT+AGG 0.322961 tig0015859:+30196 Msa1356760:CDS
GACCTCATGCAATACTATTA+TGG 0.322975 tig0015859:+30612 Msa1356760:three_prime_UTR
TTGAGTTTATTCCCGGATAA+TGG 0.328554 tig0015859:-29482 Msa1356750:intron
ATTTAACTGTTGCGGCAAAA+TGG 0.329724 tig0015859:+30005 Msa1356760:CDS
TCCTCCTGTTATAGGATCAT+TGG 0.342440 tig0015859:-30856 Msa1356770:CDS
TTGATGTTTGAGTTTATTCC+CGG 0.352803 tig0015859:-29489 Msa1356750:intron
GCTTGTGCTTCAGAAATCTA+AGG 0.355699 tig0015859:-31312 None:intergenic
GACTCACATTCAGCTGCATT+TGG 0.356576 tig0015859:-30504 Msa1356750:intron
GTTACCAAAACTATGCTTCC+AGG 0.364460 tig0015859:-30328 Msa1356750:intron
TTACAACACTTGAAAGAGTT+AGG 0.368447 tig0015859:-30364 Msa1356750:intron
CCCTCTCGTTGTTGGATACA+TGG 0.370228 tig0015859:-30830 Msa1356770:CDS
CAGAGTTTCATGCCATTATC+CGG 0.370820 tig0015859:+29470 Msa1356760:CDS
GGCCGGCTATAGGCGACTAT+TGG 0.377649 tig0015859:+30888 Msa1356760:three_prime_UTR
AGCTATCTAGTAGTGGATTA+AGG 0.384056 tig0015859:-30670 None:intergenic
ACCTTCATCAATCCATTTCA+AGG 0.398951 tig0015859:+29789 Msa1356760:CDS
ATTGGTTGCCCTCTCGTTGT+TGG 0.399335 tig0015859:-30838 Msa1356770:CDS
CAAACCTGGAAGCATAGTTT+TGG 0.405840 tig0015859:+30324 Msa1356760:CDS
CAATCCATTTCAAGGAAAGA+AGG 0.408899 tig0015859:+29797 Msa1356760:CDS
AAGTGGTGGAGGCCGGCTAT+AGG 0.418164 tig0015859:+30878 Msa1356760:three_prime_UTR
TTCTTACAGCAGCTTACTGC+TGG 0.418688 tig0015859:+29932 Msa1356760:CDS
AGGAACATGCATGTATGTTA+AGG 0.419513 tig0015859:-30573 None:intergenic
TATAGTTACTCACCTGCAAT+TGG 0.426851 tig0015859:+31278 Msa1356760:three_prime_UTR
AAAGATGAATCTGGCGAGAT+GGG 0.432844 tig0015859:-30936 Msa1356770:CDS
CGGATAATGGCATGAAACTC+TGG 0.438289 tig0015859:-29469 Msa1356750:intron
GCAACCAATGATCCTATAAC+AGG 0.441435 tig0015859:+30852 Msa1356760:three_prime_UTR
TACGTGTCTGTATCGTGTCT+TGG 0.447616 tig0015859:-31371 None:intergenic
ACAGCAGCTTACTGCTGGCA+TGG 0.459854 tig0015859:+29937 Msa1356760:CDS
GCCGGCTATAGGCGACTATT+GGG 0.460498 tig0015859:+30889 Msa1356760:three_prime_UTR
ACATACATGCATGTTCCTGC+AGG 0.462917 tig0015859:+30578 Msa1356760:three_prime_UTR
TCCTTGAAATGGATTGATGA+AGG 0.467392 tig0015859:-29790 Msa1356750:intron
ACCAATTGAATCAATCCAAA+AGG 0.487395 tig0015859:-30421 Msa1356750:intron
GATCCATTCACTTGGTAGAC+CGG 0.491216 tig0015859:+31186 Msa1356760:three_prime_UTR
GGAACATGCATGTATGTTAA+GGG 0.494614 tig0015859:-30572 None:intergenic
GAACAAATCCCATTCACAAC+AGG 0.495206 tig0015859:+30147 Msa1356760:CDS
AAGTGATCTACTATCTCTGC+TGG 0.496660 tig0015859:-29690 Msa1356750:intron
AGCTGCAGCCTGTTGTGAAT+GGG 0.499013 tig0015859:-30155 Msa1356750:intron
AAGCTGCAGCCTGTTGTGAA+TGG 0.506105 tig0015859:-30156 Msa1356750:intron
AAGCCGGTCTACCAAGTGAA+TGG 0.508284 tig0015859:-31189 Msa1356770:CDS
AAAGGAAACAAAAGTACAGT+TGG 0.510946 tig0015859:-30403 Msa1356750:intron
CTAAGACTATATTTAAGTAG+AGG 0.515504 tig0015859:+30759 Msa1356760:three_prime_UTR
AATGGCATGAAACTCTGGTT+GGG 0.526617 tig0015859:-29464 Msa1356750:intron
AAAAGATGAATCTGGCGAGA+TGG 0.530203 tig0015859:-30937 Msa1356770:CDS
GATCCTATAACAGGAGGAAG+TGG 0.536218 tig0015859:+30861 Msa1356760:three_prime_UTR
CAGGAGGAAGTGGTGGAGGC+CGG 0.536906 tig0015859:+30871 Msa1356760:three_prime_UTR
CAGCAGCTTACTGCTGGCAT+GGG 0.537521 tig0015859:+29938 Msa1356760:CDS
TAATGGCATGAAACTCTGGT+TGG 0.544699 tig0015859:-29465 Msa1356750:intron
AGAGTTTCATGCCATTATCC+GGG 0.545323 tig0015859:+29471 Msa1356760:CDS
ACCCAATAGTCGCCTATAGC+CGG 0.547078 tig0015859:-30890 Msa1356770:CDS
GGGAGATGATTTAACTGTTG+CGG 0.550185 tig0015859:+29997 Msa1356760:CDS
CTACCAAGTGAATGGATCAC+AGG 0.559874 tig0015859:-31181 Msa1356770:CDS
AGAAACATTACAAATGGATG+TGG 0.563651 tig0015859:+29431 None:intergenic
TAGATAGCTACATAAAGCTA+TGG 0.564545 tig0015859:+30684 Msa1356760:three_prime_UTR
TGCTGTCCTCATCAGTGCTG+CGG 0.577568 tig0015859:+30802 Msa1356760:three_prime_UTR
GTCATCAAACAATGAAGTCA+CGG 0.578442 tig0015859:-30478 Msa1356750:intron
ATAACAGGAGGAAGTGGTGG+AGG 0.586799 tig0015859:+30867 Msa1356760:three_prime_UTR
ATTGAATCACTAGTCAAACC+TGG 0.588394 tig0015859:+30310 Msa1356760:CDS
CCTATAACAGGAGGAAGTGG+TGG 0.596015 tig0015859:+30864 Msa1356760:three_prime_UTR
GTGGAACCGCAGCACTGATG+AGG 0.597213 tig0015859:-30808 Msa1356770:CDS
GTTGCGGCAAAATGGCACTT+AGG 0.602225 tig0015859:+30013 Msa1356760:CDS
GGTCTTCTTAAGAACCCTGC+AGG 0.602800 tig0015859:-30593 None:intergenic
TCTAGTAGTGGATTAAGGAA+AGG 0.610402 tig0015859:-30665 None:intergenic
TTTATGTAGCTATCTAGTAG+TGG 0.616283 tig0015859:-30677 None:intergenic
TACCCTGTGATCCATTCACT+TGG 0.621091 tig0015859:+31178 Msa1356760:three_prime_UTR
TTTGCTAGGCGAAAAGCTGA+GGG 0.630682 tig0015859:-29523 Msa1356750:intron
CATACATGCATGTTCCTGCA+GGG 0.632925 tig0015859:+30579 Msa1356760:three_prime_UTR
ATAGTCAGAAATATATGCGA+GGG 0.636276 tig0015859:+29977 Msa1356760:CDS
AAGATGAATCTGGCGAGATG+GGG 0.637726 tig0015859:-30935 Msa1356770:CDS
ATCCATAATAGTATTGCATG+AGG 0.639529 tig0015859:-30614 None:intergenic
CATAGTCAGAAATATATGCG+AGG 0.642978 tig0015859:+29976 Msa1356760:CDS
ACCAATGATCCTATAACAGG+AGG 0.648824 tig0015859:+30855 Msa1356760:three_prime_UTR
ACCATGTATCCAACAACGAG+AGG 0.650347 tig0015859:+30829 Msa1356760:three_prime_UTR
CCATGTATCCAACAACGAGA+GGG 0.655825 tig0015859:+30830 Msa1356760:three_prime_UTR
AGCTGAATGTGAGTCAAACA+TGG 0.656790 tig0015859:+30512 Msa1356760:three_prime_UTR
CTATAGATAAAGTTCATGCG+TGG 0.662656 tig0015859:-31252 None:intergenic
TACCAAGTGAATGGATCACA+GGG 0.666568 tig0015859:-31180 Msa1356770:intron
TCTCGTTGTTGGATACATGG+TGG 0.695822 tig0015859:-30827 Msa1356770:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GCTTATATCATTTATACTTT+AGG - tig0015859:30089-30108 Msa1356750:intron 20.0%
!!! TTTCTTCTACTTTTTCTAAT+TGG - tig0015859:30185-30204 Msa1356750:intron 20.0%
!!! ATGATATTTTGCATTACTAT+AGG - tig0015859:30987-31006 Msa1356770:intron 20.0%
!! GCTTATATCATTTATACTTT+AGG - tig0015859:30089-30108 Msa1356750:intron 20.0%
!!! TTTCTTCTACTTTTTCTAAT+TGG - tig0015859:30185-30204 Msa1356750:intron 20.0%
!!! ATGATATTTTGCATTACTAT+AGG - tig0015859:30987-31006 Msa1356770:intron 20.0%
!! TTTGAATTTTTCCCGAATTT+GGG - tig0015859:29666-29685 Msa1356750:intron 25.0%
! TGAAAAAGAGCTAAAACAAT+TGG + tig0015859:29728-29747 Msa1356760:CDS 25.0%
! TAATCTAAACATAACATTGC+AGG + tig0015859:29898-29917 Msa1356760:intron 25.0%
! AAAAGCAATAAATCTAGGAA+AGG - tig0015859:30046-30065 Msa1356750:intron 25.0%
! TAGAAGAAAACATGACAATT+AGG + tig0015859:30196-30215 Msa1356760:CDS 25.0%
!! GTATGTTAAGGGATATTTTT+GGG - tig0015859:30564-30583 Msa1356750:intron 25.0%
!! TGTATGTTAAGGGATATTTT+TGG - tig0015859:30565-30584 Msa1356750:intron 25.0%
! CTAAGACTATATTTAAGTAG+AGG + tig0015859:30759-30778 Msa1356760:three_prime_UTR 25.0%
! AAACAAGAAAAAGATGAATC+TGG - tig0015859:30948-30967 Msa1356770:CDS 25.0%
!!! TCATCTTTTTCTTGTTTCTT+AGG + tig0015859:30951-30970 Msa1356760:three_prime_UTR 25.0%
! TTGCATTACTATAGGTATAT+TGG - tig0015859:30979-30998 Msa1356770:intron 25.0%
!! TTTGAATTTTTCCCGAATTT+GGG - tig0015859:29666-29685 Msa1356750:intron 25.0%
! TGAAAAAGAGCTAAAACAAT+TGG + tig0015859:29728-29747 Msa1356760:CDS 25.0%
! TAATCTAAACATAACATTGC+AGG + tig0015859:29898-29917 Msa1356760:intron 25.0%
! AAAAGCAATAAATCTAGGAA+AGG - tig0015859:30046-30065 Msa1356750:intron 25.0%
! TAGAAGAAAACATGACAATT+AGG + tig0015859:30196-30215 Msa1356760:CDS 25.0%
!! GTATGTTAAGGGATATTTTT+GGG - tig0015859:30564-30583 Msa1356750:intron 25.0%
!! TGTATGTTAAGGGATATTTT+TGG - tig0015859:30565-30584 Msa1356750:intron 25.0%
! CTAAGACTATATTTAAGTAG+AGG + tig0015859:30759-30778 Msa1356760:three_prime_UTR 25.0%
! AAACAAGAAAAAGATGAATC+TGG - tig0015859:30948-30967 Msa1356770:CDS 25.0%
!!! TCATCTTTTTCTTGTTTCTT+AGG + tig0015859:30951-30970 Msa1356760:three_prime_UTR 25.0%
! TTGCATTACTATAGGTATAT+TGG - tig0015859:30979-30998 Msa1356770:intron 25.0%
! TTGATGTTTGAGTTTATTCC+CGG - tig0015859:29492-29511 Msa1356750:intron 30.0%
AGAATGTCAAAAACAGAACT+TGG + tig0015859:29601-29620 Msa1356760:CDS 30.0%
GAATGTCAAAAACAGAACTT+GGG + tig0015859:29602-29621 Msa1356760:CDS 30.0%
AATGTCAAAAACAGAACTTG+GGG + tig0015859:29603-29622 Msa1356760:CDS 30.0%
TCAGCCATATTATCAAAAAG+AGG + tig0015859:29639-29658 Msa1356760:CDS 30.0%
! TTGAATTTTTCCCGAATTTG+GGG - tig0015859:29665-29684 Msa1356750:intron 30.0%
! GTTTGAATTTTTCCCGAATT+TGG - tig0015859:29667-29686 Msa1356750:intron 30.0%
GTTATTAGAACTTAGAAGGA+TGG - tig0015859:29835-29854 Msa1356750:intron 30.0%
CTAGGTTATTAGAACTTAGA+AGG - tig0015859:29839-29858 Msa1356750:intron 30.0%
TGACTATGAATTTCACATTC+TGG - tig0015859:29964-29983 Msa1356750:intron 30.0%
ATAGTCAGAAATATATGCGA+GGG + tig0015859:29977-29996 Msa1356760:CDS 30.0%
AGCAGAAAAGCAATAAATCT+AGG - tig0015859:30051-30070 Msa1356750:intron 30.0%
CTCACTATGTAATTGAATTC+AGG + tig0015859:30272-30291 Msa1356760:intron 30.0%
! AAAGAGTTAGGTTCTGTTTT+AGG - tig0015859:30355-30374 Msa1356750:intron 30.0%
TTACAACACTTGAAAGAGTT+AGG - tig0015859:30367-30386 Msa1356750:intron 30.0%
! AAAGGAAACAAAAGTACAGT+TGG - tig0015859:30406-30425 Msa1356750:intron 30.0%
!! ACTGTACTTTTGTTTCCTTT+TGG + tig0015859:30406-30425 Msa1356760:three_prime_UTR 30.0%
!! TCCTTTTGGATTGATTCAAT+TGG + tig0015859:30420-30439 Msa1356760:three_prime_UTR 30.0%
ACCAATTGAATCAATCCAAA+AGG - tig0015859:30424-30443 Msa1356750:intron 30.0%
ATCCATAATAGTATTGCATG+AGG - tig0015859:30617-30636 None:intergenic 30.0%
TTTATGTAGCTATCTAGTAG+TGG - tig0015859:30680-30699 None:intergenic 30.0%
TAGATAGCTACATAAAGCTA+TGG + tig0015859:30684-30703 Msa1356760:three_prime_UTR 30.0%
!! TAGTTTGAGTGTTGAACAAT+GGG - tig0015859:31070-31089 Msa1356770:intron 30.0%
TGCAAAAAACAGATTTAAGC+CGG - tig0015859:31208-31227 Msa1356770:CDS 30.0%
! TTGATGTTTGAGTTTATTCC+CGG - tig0015859:29492-29511 Msa1356750:intron 30.0%
AGAATGTCAAAAACAGAACT+TGG + tig0015859:29601-29620 Msa1356760:CDS 30.0%
GAATGTCAAAAACAGAACTT+GGG + tig0015859:29602-29621 Msa1356760:CDS 30.0%
AATGTCAAAAACAGAACTTG+GGG + tig0015859:29603-29622 Msa1356760:CDS 30.0%
TCAGCCATATTATCAAAAAG+AGG + tig0015859:29639-29658 Msa1356760:CDS 30.0%
! TTGAATTTTTCCCGAATTTG+GGG - tig0015859:29665-29684 Msa1356750:intron 30.0%
! GTTTGAATTTTTCCCGAATT+TGG - tig0015859:29667-29686 Msa1356750:intron 30.0%
GTTATTAGAACTTAGAAGGA+TGG - tig0015859:29835-29854 Msa1356750:intron 30.0%
CTAGGTTATTAGAACTTAGA+AGG - tig0015859:29839-29858 Msa1356750:intron 30.0%
TGACTATGAATTTCACATTC+TGG - tig0015859:29964-29983 Msa1356750:intron 30.0%
ATAGTCAGAAATATATGCGA+GGG + tig0015859:29977-29996 Msa1356760:CDS 30.0%
AGCAGAAAAGCAATAAATCT+AGG - tig0015859:30051-30070 Msa1356750:intron 30.0%
CTCACTATGTAATTGAATTC+AGG + tig0015859:30272-30291 Msa1356760:intron 30.0%
! AAAGAGTTAGGTTCTGTTTT+AGG - tig0015859:30355-30374 Msa1356750:intron 30.0%
TTACAACACTTGAAAGAGTT+AGG - tig0015859:30367-30386 Msa1356750:intron 30.0%
! AAAGGAAACAAAAGTACAGT+TGG - tig0015859:30406-30425 Msa1356750:intron 30.0%
!! ACTGTACTTTTGTTTCCTTT+TGG + tig0015859:30406-30425 Msa1356760:three_prime_UTR 30.0%
!! TCCTTTTGGATTGATTCAAT+TGG + tig0015859:30420-30439 Msa1356760:three_prime_UTR 30.0%
ACCAATTGAATCAATCCAAA+AGG - tig0015859:30424-30443 Msa1356750:intron 30.0%
ATCCATAATAGTATTGCATG+AGG - tig0015859:30617-30636 None:intergenic 30.0%
TTTATGTAGCTATCTAGTAG+TGG - tig0015859:30680-30699 None:intergenic 30.0%
TAGATAGCTACATAAAGCTA+TGG + tig0015859:30684-30703 Msa1356760:three_prime_UTR 30.0%
!! TAGTTTGAGTGTTGAACAAT+GGG - tig0015859:31070-31089 Msa1356770:intron 30.0%
TGCAAAAAACAGATTTAAGC+CGG - tig0015859:31208-31227 Msa1356770:CDS 30.0%
TTGAGTTTATTCCCGGATAA+TGG - tig0015859:29485-29504 Msa1356750:intron 35.0%
! AAGTTGCATGATGTTTTGCT+AGG - tig0015859:29540-29559 Msa1356750:intron 35.0%
ATGCAACTTGCACAAAAAGA+TGG + tig0015859:29551-29570 Msa1356760:CDS 35.0%
ACCTTCATCAATCCATTTCA+AGG + tig0015859:29789-29808 Msa1356760:CDS 35.0%
!! TCCTTGAAATGGATTGATGA+AGG - tig0015859:29793-29812 Msa1356750:intron 35.0%
CAATCCATTTCAAGGAAAGA+AGG + tig0015859:29797-29816 Msa1356760:CDS 35.0%
CAAACCTTCTTTCCTTGAAA+TGG - tig0015859:29804-29823 Msa1356750:intron 35.0%
CATAGTCAGAAATATATGCG+AGG + tig0015859:29976-29995 Msa1356760:CDS 35.0%
ATTTAACTGTTGCGGCAAAA+TGG + tig0015859:30005-30024 Msa1356760:CDS 35.0%
! AAGCCTGACGAATATCTTTT+TGG + tig0015859:30105-30124 Msa1356760:intron 35.0%
ATGCCAAAAAGATATTCGTC+AGG - tig0015859:30111-30130 Msa1356750:intron 35.0%
!! TGACGAATATCTTTTTGGCA+TGG + tig0015859:30110-30129 Msa1356760:intron 35.0%
!! ATCTTTTTGGCATGGTAGAA+TGG + tig0015859:30118-30137 Msa1356760:intron 35.0%
ATTGAATCACTAGTCAAACC+TGG + tig0015859:30310-30329 Msa1356760:CDS 35.0%
GTCATCAAACAATGAAGTCA+CGG - tig0015859:30481-30500 Msa1356750:intron 35.0%
GGAACATGCATGTATGTTAA+GGG - tig0015859:30575-30594 None:intergenic 35.0%
AGGAACATGCATGTATGTTA+AGG - tig0015859:30576-30595 None:intergenic 35.0%
GACCTCATGCAATACTATTA+TGG + tig0015859:30612-30631 Msa1356760:three_prime_UTR 35.0%
!! TCTAGTAGTGGATTAAGGAA+AGG - tig0015859:30668-30687 None:intergenic 35.0%
!! AGCTATCTAGTAGTGGATTA+AGG - tig0015859:30673-30692 None:intergenic 35.0%
!! CTAGTTTGAGTGTTGAACAA+TGG - tig0015859:31071-31090 Msa1356770:intron 35.0%
CTATAGATAAAGTTCATGCG+TGG - tig0015859:31255-31274 None:intergenic 35.0%
TATAGTTACTCACCTGCAAT+TGG + tig0015859:31278-31297 Msa1356760:three_prime_UTR 35.0%
!!! GCTTGTTTTATTCCAATTGC+AGG - tig0015859:31293-31312 None:intergenic 35.0%
AAGCACAAGCATGAAAAACT+CGG + tig0015859:31325-31344 Msa1356760:three_prime_UTR 35.0%
TTGAGTTTATTCCCGGATAA+TGG - tig0015859:29485-29504 Msa1356750:intron 35.0%
! AAGTTGCATGATGTTTTGCT+AGG - tig0015859:29540-29559 Msa1356750:intron 35.0%
ATGCAACTTGCACAAAAAGA+TGG + tig0015859:29551-29570 Msa1356760:CDS 35.0%
ACCTTCATCAATCCATTTCA+AGG + tig0015859:29789-29808 Msa1356760:CDS 35.0%
!! TCCTTGAAATGGATTGATGA+AGG - tig0015859:29793-29812 Msa1356750:intron 35.0%
CAATCCATTTCAAGGAAAGA+AGG + tig0015859:29797-29816 Msa1356760:CDS 35.0%
CAAACCTTCTTTCCTTGAAA+TGG - tig0015859:29804-29823 Msa1356750:intron 35.0%
CATAGTCAGAAATATATGCG+AGG + tig0015859:29976-29995 Msa1356760:CDS 35.0%
ATTTAACTGTTGCGGCAAAA+TGG + tig0015859:30005-30024 Msa1356760:CDS 35.0%
! AAGCCTGACGAATATCTTTT+TGG + tig0015859:30105-30124 Msa1356760:intron 35.0%
ATGCCAAAAAGATATTCGTC+AGG - tig0015859:30111-30130 Msa1356750:intron 35.0%
!! TGACGAATATCTTTTTGGCA+TGG + tig0015859:30110-30129 Msa1356760:intron 35.0%
!! ATCTTTTTGGCATGGTAGAA+TGG + tig0015859:30118-30137 Msa1356760:intron 35.0%
ATTGAATCACTAGTCAAACC+TGG + tig0015859:30310-30329 Msa1356760:CDS 35.0%
GTCATCAAACAATGAAGTCA+CGG - tig0015859:30481-30500 Msa1356750:intron 35.0%
GGAACATGCATGTATGTTAA+GGG - tig0015859:30575-30594 None:intergenic 35.0%
AGGAACATGCATGTATGTTA+AGG - tig0015859:30576-30595 None:intergenic 35.0%
GACCTCATGCAATACTATTA+TGG + tig0015859:30612-30631 Msa1356760:three_prime_UTR 35.0%
!! TCTAGTAGTGGATTAAGGAA+AGG - tig0015859:30668-30687 None:intergenic 35.0%
!! AGCTATCTAGTAGTGGATTA+AGG - tig0015859:30673-30692 None:intergenic 35.0%
!! CTAGTTTGAGTGTTGAACAA+TGG - tig0015859:31071-31090 Msa1356770:intron 35.0%
CTATAGATAAAGTTCATGCG+TGG - tig0015859:31255-31274 None:intergenic 35.0%
TATAGTTACTCACCTGCAAT+TGG + tig0015859:31278-31297 Msa1356760:three_prime_UTR 35.0%
!!! GCTTGTTTTATTCCAATTGC+AGG - tig0015859:31293-31312 None:intergenic 35.0%
AAGCACAAGCATGAAAAACT+CGG + tig0015859:31325-31344 Msa1356760:three_prime_UTR 35.0%
TGAAACTCTGGTTGGGAATT+TGG - tig0015859:29460-29479 Msa1356750:intron 40.0%
AATGGCATGAAACTCTGGTT+GGG - tig0015859:29467-29486 Msa1356750:intron 40.0%
TAATGGCATGAAACTCTGGT+TGG - tig0015859:29468-29487 Msa1356750:intron 40.0%
CAGAGTTTCATGCCATTATC+CGG + tig0015859:29470-29489 Msa1356760:CDS 40.0%
AGAGTTTCATGCCATTATCC+GGG + tig0015859:29471-29490 Msa1356760:CDS 40.0%
!! GGGGCCTCTTTTTGATAATA+TGG - tig0015859:29646-29665 Msa1356750:intron 40.0%
TCAAAAAGAGGCCCCAAATT+CGG + tig0015859:29651-29670 Msa1356760:CDS 40.0%
AAGTGATCTACTATCTCTGC+TGG - tig0015859:29693-29712 Msa1356750:intron 40.0%
AGCTGTTTACTCGATGCAAT+CGG - tig0015859:29870-29889 Msa1356750:intron 40.0%
GGGAGATGATTTAACTGTTG+CGG + tig0015859:29997-30016 Msa1356760:CDS 40.0%
TGGCATGGTAGAATGGAAAA+AGG + tig0015859:30125-30144 Msa1356760:intron 40.0%
GAACAAATCCCATTCACAAC+AGG + tig0015859:30147-30166 Msa1356760:CDS 40.0%
!! CAAACCTGGAAGCATAGTTT+TGG + tig0015859:30324-30343 Msa1356760:CDS 40.0%
GTTACCAAAACTATGCTTCC+AGG - tig0015859:30331-30350 Msa1356750:intron 40.0%
AGCTGAATGTGAGTCAAACA+TGG + tig0015859:30512-30531 Msa1356760:three_prime_UTR 40.0%
GCAACCAATGATCCTATAAC+AGG + tig0015859:30852-30871 Msa1356760:three_prime_UTR 40.0%
ACCAATGATCCTATAACAGG+AGG + tig0015859:30855-30874 Msa1356760:three_prime_UTR 40.0%
TCCTCCTGTTATAGGATCAT+TGG - tig0015859:30859-30878 Msa1356770:CDS 40.0%
AAAGATGAATCTGGCGAGAT+GGG - tig0015859:30939-30958 Msa1356770:CDS 40.0%
AAAAGATGAATCTGGCGAGA+TGG - tig0015859:30940-30959 Msa1356770:CDS 40.0%
TACCAAGTGAATGGATCACA+GGG - tig0015859:31183-31202 Msa1356770:CDS 40.0%
GCTTGTGCTTCAGAAATCTA+AGG - tig0015859:31315-31334 None:intergenic 40.0%
TGAAACTCTGGTTGGGAATT+TGG - tig0015859:29460-29479 Msa1356750:intron 40.0%
AATGGCATGAAACTCTGGTT+GGG - tig0015859:29467-29486 Msa1356750:intron 40.0%
TAATGGCATGAAACTCTGGT+TGG - tig0015859:29468-29487 Msa1356750:intron 40.0%
CAGAGTTTCATGCCATTATC+CGG + tig0015859:29470-29489 Msa1356760:CDS 40.0%
AGAGTTTCATGCCATTATCC+GGG + tig0015859:29471-29490 Msa1356760:CDS 40.0%
!! GGGGCCTCTTTTTGATAATA+TGG - tig0015859:29646-29665 Msa1356750:intron 40.0%
TCAAAAAGAGGCCCCAAATT+CGG + tig0015859:29651-29670 Msa1356760:CDS 40.0%
AAGTGATCTACTATCTCTGC+TGG - tig0015859:29693-29712 Msa1356750:intron 40.0%
AGCTGTTTACTCGATGCAAT+CGG - tig0015859:29870-29889 Msa1356750:intron 40.0%
GGGAGATGATTTAACTGTTG+CGG + tig0015859:29997-30016 Msa1356760:CDS 40.0%
TGGCATGGTAGAATGGAAAA+AGG + tig0015859:30125-30144 Msa1356760:intron 40.0%
GAACAAATCCCATTCACAAC+AGG + tig0015859:30147-30166 Msa1356760:CDS 40.0%
!! CAAACCTGGAAGCATAGTTT+TGG + tig0015859:30324-30343 Msa1356760:CDS 40.0%
GTTACCAAAACTATGCTTCC+AGG - tig0015859:30331-30350 Msa1356750:intron 40.0%
AGCTGAATGTGAGTCAAACA+TGG + tig0015859:30512-30531 Msa1356760:three_prime_UTR 40.0%
GCAACCAATGATCCTATAAC+AGG + tig0015859:30852-30871 Msa1356760:three_prime_UTR 40.0%
ACCAATGATCCTATAACAGG+AGG + tig0015859:30855-30874 Msa1356760:three_prime_UTR 40.0%
TCCTCCTGTTATAGGATCAT+TGG - tig0015859:30859-30878 Msa1356770:CDS 40.0%
AAAGATGAATCTGGCGAGAT+GGG - tig0015859:30939-30958 Msa1356770:CDS 40.0%
AAAAGATGAATCTGGCGAGA+TGG - tig0015859:30940-30959 Msa1356770:CDS 40.0%
TACCAAGTGAATGGATCACA+GGG - tig0015859:31183-31202 Msa1356770:CDS 40.0%
GCTTGTGCTTCAGAAATCTA+AGG - tig0015859:31315-31334 None:intergenic 40.0%
! CGGATAATGGCATGAAACTC+TGG - tig0015859:29472-29491 Msa1356750:intron 45.0%
TTTGCTAGGCGAAAAGCTGA+GGG - tig0015859:29526-29545 Msa1356750:intron 45.0%
! TTTTGCTAGGCGAAAAGCTG+AGG - tig0015859:29527-29546 Msa1356750:intron 45.0%
CAAAAAGAGGCCCCAAATTC+GGG + tig0015859:29652-29671 Msa1356760:CDS 45.0%
ATGCAATCGGGGTTACTTCT+AGG - tig0015859:29857-29876 Msa1356750:intron 45.0%
CTGTTTACTCGATGCAATCG+GGG - tig0015859:29868-29887 Msa1356750:intron 45.0%
GCTGTTTACTCGATGCAATC+GGG - tig0015859:29869-29888 Msa1356750:intron 45.0%
TTCTTACAGCAGCTTACTGC+TGG + tig0015859:29932-29951 Msa1356760:CDS 45.0%
GACTCACATTCAGCTGCATT+TGG - tig0015859:30507-30526 Msa1356750:intron 45.0%
ACATACATGCATGTTCCTGC+AGG + tig0015859:30578-30597 Msa1356760:three_prime_UTR 45.0%
CATACATGCATGTTCCTGCA+GGG + tig0015859:30579-30598 Msa1356760:three_prime_UTR 45.0%
TCTCGTTGTTGGATACATGG+TGG - tig0015859:30830-30849 Msa1356770:CDS 45.0%
ACCATGTATCCAACAACGAG+AGG + tig0015859:30829-30848 Msa1356760:three_prime_UTR 45.0%
CCATGTATCCAACAACGAGA+GGG + tig0015859:30830-30849 Msa1356760:three_prime_UTR 45.0%
GATCCTATAACAGGAGGAAG+TGG + tig0015859:30861-30880 Msa1356760:three_prime_UTR 45.0%
AAGATGAATCTGGCGAGATG+GGG - tig0015859:30938-30957 Msa1356770:CDS 45.0%
TACCCTGTGATCCATTCACT+TGG + tig0015859:31178-31197 Msa1356760:three_prime_UTR 45.0%
CTACCAAGTGAATGGATCAC+AGG - tig0015859:31184-31203 Msa1356770:CDS 45.0%
GATCCATTCACTTGGTAGAC+CGG + tig0015859:31186-31205 Msa1356760:three_prime_UTR 45.0%
TACGTGTCTGTATCGTGTCT+TGG - tig0015859:31374-31393 None:intergenic 45.0%
! CGGATAATGGCATGAAACTC+TGG - tig0015859:29472-29491 Msa1356750:intron 45.0%
TTTGCTAGGCGAAAAGCTGA+GGG - tig0015859:29526-29545 Msa1356750:intron 45.0%
! TTTTGCTAGGCGAAAAGCTG+AGG - tig0015859:29527-29546 Msa1356750:intron 45.0%
CAAAAAGAGGCCCCAAATTC+GGG + tig0015859:29652-29671 Msa1356760:CDS 45.0%
ATGCAATCGGGGTTACTTCT+AGG - tig0015859:29857-29876 Msa1356750:intron 45.0%
CTGTTTACTCGATGCAATCG+GGG - tig0015859:29868-29887 Msa1356750:intron 45.0%
GCTGTTTACTCGATGCAATC+GGG - tig0015859:29869-29888 Msa1356750:intron 45.0%
TTCTTACAGCAGCTTACTGC+TGG + tig0015859:29932-29951 Msa1356760:CDS 45.0%
GACTCACATTCAGCTGCATT+TGG - tig0015859:30507-30526 Msa1356750:intron 45.0%
ACATACATGCATGTTCCTGC+AGG + tig0015859:30578-30597 Msa1356760:three_prime_UTR 45.0%
CATACATGCATGTTCCTGCA+GGG + tig0015859:30579-30598 Msa1356760:three_prime_UTR 45.0%
TCTCGTTGTTGGATACATGG+TGG - tig0015859:30830-30849 Msa1356770:CDS 45.0%
ACCATGTATCCAACAACGAG+AGG + tig0015859:30829-30848 Msa1356760:three_prime_UTR 45.0%
CCATGTATCCAACAACGAGA+GGG + tig0015859:30830-30849 Msa1356760:three_prime_UTR 45.0%
GATCCTATAACAGGAGGAAG+TGG + tig0015859:30861-30880 Msa1356760:three_prime_UTR 45.0%
AAGATGAATCTGGCGAGATG+GGG - tig0015859:30938-30957 Msa1356770:CDS 45.0%
TACCCTGTGATCCATTCACT+TGG + tig0015859:31178-31197 Msa1356760:three_prime_UTR 45.0%
CTACCAAGTGAATGGATCAC+AGG - tig0015859:31184-31203 Msa1356770:CDS 45.0%
GATCCATTCACTTGGTAGAC+CGG + tig0015859:31186-31205 Msa1356760:three_prime_UTR 45.0%
TACGTGTCTGTATCGTGTCT+TGG - tig0015859:31374-31393 None:intergenic 45.0%
GTTGCGGCAAAATGGCACTT+AGG + tig0015859:30013-30032 Msa1356760:CDS 50.0%
AGCTGCAGCCTGTTGTGAAT+GGG - tig0015859:30158-30177 Msa1356750:intron 50.0%
AAGCTGCAGCCTGTTGTGAA+TGG - tig0015859:30159-30178 Msa1356750:intron 50.0%
GGTCTTCTTAAGAACCCTGC+AGG - tig0015859:30596-30615 None:intergenic 50.0%
CCCTCTCGTTGTTGGATACA+TGG - tig0015859:30833-30852 Msa1356770:CDS 50.0%
ATTGGTTGCCCTCTCGTTGT+TGG - tig0015859:30841-30860 Msa1356770:CDS 50.0%
CCACCACTTCCTCCTGTTAT+AGG - tig0015859:30867-30886 Msa1356770:CDS 50.0%
CCTATAACAGGAGGAAGTGG+TGG + tig0015859:30864-30883 Msa1356760:three_prime_UTR 50.0%
ATAACAGGAGGAAGTGGTGG+AGG + tig0015859:30867-30886 Msa1356760:three_prime_UTR 50.0%
ACCCAATAGTCGCCTATAGC+CGG - tig0015859:30893-30912 Msa1356770:CDS 50.0%
AAGCCGGTCTACCAAGTGAA+TGG - tig0015859:31192-31211 Msa1356770:CDS 50.0%
GTTGCGGCAAAATGGCACTT+AGG + tig0015859:30013-30032 Msa1356760:CDS 50.0%
AGCTGCAGCCTGTTGTGAAT+GGG - tig0015859:30158-30177 Msa1356750:intron 50.0%
AAGCTGCAGCCTGTTGTGAA+TGG - tig0015859:30159-30178 Msa1356750:intron 50.0%
GGTCTTCTTAAGAACCCTGC+AGG - tig0015859:30596-30615 None:intergenic 50.0%
CCCTCTCGTTGTTGGATACA+TGG - tig0015859:30833-30852 Msa1356770:CDS 50.0%
ATTGGTTGCCCTCTCGTTGT+TGG - tig0015859:30841-30860 Msa1356770:CDS 50.0%
CCACCACTTCCTCCTGTTAT+AGG - tig0015859:30867-30886 Msa1356770:CDS 50.0%
CCTATAACAGGAGGAAGTGG+TGG + tig0015859:30864-30883 Msa1356760:three_prime_UTR 50.0%
ATAACAGGAGGAAGTGGTGG+AGG + tig0015859:30867-30886 Msa1356760:three_prime_UTR 50.0%
ACCCAATAGTCGCCTATAGC+CGG - tig0015859:30893-30912 Msa1356770:CDS 50.0%
AAGCCGGTCTACCAAGTGAA+TGG - tig0015859:31192-31211 Msa1356770:CDS 50.0%
ACAGCAGCTTACTGCTGGCA+TGG + tig0015859:29937-29956 Msa1356760:CDS 55.0%
CAGCAGCTTACTGCTGGCAT+GGG + tig0015859:29938-29957 Msa1356760:CDS 55.0%
TGCTGTCCTCATCAGTGCTG+CGG + tig0015859:30802-30821 Msa1356760:three_prime_UTR 55.0%
GCCGGCTATAGGCGACTATT+GGG + tig0015859:30889-30908 Msa1356760:three_prime_UTR 55.0%
ACAGCAGCTTACTGCTGGCA+TGG + tig0015859:29937-29956 Msa1356760:CDS 55.0%
CAGCAGCTTACTGCTGGCAT+GGG + tig0015859:29938-29957 Msa1356760:CDS 55.0%
TGCTGTCCTCATCAGTGCTG+CGG + tig0015859:30802-30821 Msa1356760:three_prime_UTR 55.0%
GCCGGCTATAGGCGACTATT+GGG + tig0015859:30889-30908 Msa1356760:three_prime_UTR 55.0%
!! GTGGAACCGCAGCACTGATG+AGG - tig0015859:30811-30830 Msa1356770:CDS 60.0%
! AAGTGGTGGAGGCCGGCTAT+AGG + tig0015859:30878-30897 Msa1356760:three_prime_UTR 60.0%
GGCCGGCTATAGGCGACTAT+TGG + tig0015859:30888-30907 Msa1356760:three_prime_UTR 60.0%
!! GTGGAACCGCAGCACTGATG+AGG - tig0015859:30811-30830 Msa1356770:CDS 60.0%
! AAGTGGTGGAGGCCGGCTAT+AGG + tig0015859:30878-30897 Msa1356760:three_prime_UTR 60.0%
GGCCGGCTATAGGCGACTAT+TGG + tig0015859:30888-30907 Msa1356760:three_prime_UTR 60.0%
! CAGGAGGAAGTGGTGGAGGC+CGG + tig0015859:30871-30890 Msa1356760:three_prime_UTR 65.0%
! CAGGAGGAAGTGGTGGAGGC+CGG + tig0015859:30871-30890 Msa1356760:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
tig0015859 gene 29444 31412 29444 ID=Msa1356760;Name=Msa1356760
tig0015859 mRNA 29444 31412 29444 ID=Msa1356760-mRNA-1;Parent=Msa1356760;Name=Msa1356760-mRNA-1;_AED=0.08;_eAED=0.08;_QI=0|1|0.8|1|0.75|0.6|5|817|225
tig0015859 exon 29444 29818 29444 ID=Msa1356760-mRNA-1:exon:10102;Parent=Msa1356760-mRNA-1
tig0015859 exon 29920 30034 29920 ID=Msa1356760-mRNA-1:exon:10103;Parent=Msa1356760-mRNA-1
tig0015859 exon 30136 30217 30136 ID=Msa1356760-mRNA-1:exon:10104;Parent=Msa1356760-mRNA-1
tig0015859 exon 30294 30980 30294 ID=Msa1356760-mRNA-1:exon:10105;Parent=Msa1356760-mRNA-1
tig0015859 exon 31177 31412 31177 ID=Msa1356760-mRNA-1:exon:10106;Parent=Msa1356760-mRNA-1
tig0015859 CDS 29444 29818 29444 ID=Msa1356760-mRNA-1:cds;Parent=Msa1356760-mRNA-1
tig0015859 CDS 29920 30034 29920 ID=Msa1356760-mRNA-1:cds;Parent=Msa1356760-mRNA-1
tig0015859 CDS 30136 30217 30136 ID=Msa1356760-mRNA-1:cds;Parent=Msa1356760-mRNA-1
tig0015859 CDS 30294 30399 30294 ID=Msa1356760-mRNA-1:cds;Parent=Msa1356760-mRNA-1
tig0015859 three_prime_UTR 30400 30980 30400 ID=Msa1356760-mRNA-1:three_prime_utr;Parent=Msa1356760-mRNA-1
tig0015859 three_prime_UTR 31177 31412 31177 ID=Msa1356760-mRNA-1:three_prime_utr;Parent=Msa1356760-mRNA-1
Gene Sequence

>Msa1356760

ATGGATGTGGTAGCCAAATTCCCAACCAGAGTTTCATGCCATTATCCGGGAATAAACTCAAACATCAAAAAGAGACTCGCCCTCAGCTTTTCGCCTAGCAAAACATCATGCAACTTGCACAAAAAGATGGAGATACAACAAAGAAGCATTTGTTTCAAGAATGTCAAAAACAGAACTTGGGGTAACAATAGAATTTCAGCCATATTATCAAAAAGAGGCCCCAAATTCGGGAAAAATTCAAACTCTCCAGCAGAGATAGTAGATCACTTCTACACATGTATCAATGAAAAAGAGCTAAAACAATTGGATGAATACATATCACAAGATGCTTGCTTTTATGACTATACCTTCATCAATCCATTTCAAGGAAAGAAGGAAGTGATGCATTTCTTACAGCAGCTTACTGCTGGCATGGGCCAGAATGTGAAATTCATAGTCAGAAATATATGCGAGGGAGATGATTTAACTGTTGCGGCAAAATGGCACTTAGAATGGAAAAAGGAACAAATCCCATTCACAACAGGCTGCAGCTTCTTCCAATTAGAAAAAGTAGAAGAAAACATGACAATTAGGAGAGCTGAGATATTTATTGAATCACTAGTCAAACCTGGAAGCATAGTTTTGGTAACTCCTAAAACAGAACCTAACTCTTTCAAGTGTTGTAATTCTTATACGTGA
Protein sequence

>Msa1356760

MDVVAKFPTRVSCHYPGINSNIKKRLALSFSPSKTSCNLHKKMEIQQRSICFKNVKNRTWGNNRISAILSKRGPKFGKNSNSPAEIVDHFYTCINEKELKQLDEYISQDACFYDYTFINPFQGKKEVMHFLQQLTAGMGQNVKFIVRNICEGDDLTVAAKWHLEWKKEQIPFTTGCSFFQLEKVEENMTIRRAEIFIESLVKPGSIVLVTPKTEPNSFKCCNSYT*