AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0712810

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0712810 MtrunA17_Chr5g0406001 96.584 527 18 0 1 527 1 527 0.0 1054
Msa0712810 MtrunA17_Chr8g0371371 64.600 500 164 9 31 525 31 522 0.0 644
Msa0712810 MtrunA17_Chr8g0371351 47.348 509 241 13 33 526 20 516 5.43e-154 450
Msa0712810 MtrunA17_Chr3g0124421 38.497 439 253 12 37 466 25 455 8.88e-94 295
Msa0712810 MtrunA17_Chr5g0425141 37.628 489 271 16 38 513 24 491 7.68e-90 285
Msa0712810 MtrunA17_Chr6g0484731 27.865 384 219 16 112 461 191 550 3.31e-31 127
Msa0712810 MtrunA17_Chr4g0027681 29.082 392 224 18 112 474 77 443 6.71e-31 125
Msa0712810 MtrunA17_Chr7g0221681 26.650 409 250 16 112 486 72 464 4.40e-30 123
Msa0712810 MtrunA17_Chr1g0179751 26.379 417 256 16 75 461 74 469 1.33e-29 122
Msa0712810 MtrunA17_Chr7g0265331 26.971 482 291 21 72 526 45 492 4.00e-29 120
Msa0712810 MtrunA17_Chr5g0419291 26.681 461 279 18 19 461 9 428 6.18e-27 113
Msa0712810 MtrunA17_Chr2g0320621 26.862 443 267 21 72 486 43 456 1.46e-26 112
Msa0712810 MtrunA17_Chr1g0189381 27.865 384 220 19 112 461 69 429 3.39e-26 111
Msa0712810 MtrunA17_Chr3g0095891 27.792 385 218 15 113 461 98 458 1.32e-25 109
Msa0712810 MtrunA17_Chr3g0130931 27.373 453 262 17 34 461 38 448 1.37e-25 109
Msa0712810 MtrunA17_Chr3g0130741 27.913 369 219 16 117 461 89 434 4.95e-24 104
Msa0712810 MtrunA17_Chr5g0435871 25.793 473 285 19 12 461 5 434 1.21e-23 103
Msa0712810 MtrunA17_Chr8g0384731 26.943 386 229 16 114 473 73 431 2.25e-23 102
Msa0712810 MtrunA17_Chr7g0249451 26.247 381 229 14 112 461 71 430 6.28e-23 102
Msa0712810 MtrunA17_Chr5g0442921 26.382 398 226 14 106 463 185 555 1.25e-22 101
Msa0712810 MtrunA17_Chr4g0010951 25.490 408 223 16 112 461 61 445 3.58e-21 96.3
Msa0712810 MtrunA17_Chr8g0352671 25.265 471 285 20 15 461 9 436 5.79e-21 95.5
Msa0712810 MtrunA17_Chr5g0395911 26.431 367 231 15 113 461 68 413 1.09e-20 94.4
Msa0712810 MtrunA17_Chr5g0415951 27.869 366 215 16 119 461 95 434 1.11e-20 94.7
Msa0712810 MtrunA17_Chr2g0307991 26.735 389 235 18 96 461 43 404 1.31e-20 94.0
Msa0712810 MtrunA17_Chr8g0386441 26.632 383 226 16 99 461 82 429 4.14e-20 92.8
Msa0712810 MtrunA17_Chr2g0310801 26.503 366 229 16 113 461 91 433 4.07e-19 90.1
Msa0712810 MtrunA17_Chr7g0275731 26.596 376 216 17 113 461 142 484 3.16e-18 87.4
Msa0712810 MtrunA17_Chr8g0352661 26.250 480 279 26 12 461 2 436 5.38e-18 86.7
Msa0712810 MtrunA17_Chr5g0401601 24.582 419 230 19 119 486 95 478 6.27e-18 87.0
Msa0712810 MtrunA17_Chr4g0053721 25.110 454 262 21 108 525 77 488 6.75e-18 87.0
Msa0712810 MtrunA17_Chr8g0353261 26.882 372 214 17 113 461 97 433 1.60e-17 85.1
Msa0712810 MtrunA17_Chr2g0308001 24.515 412 252 18 106 493 52 428 1.82e-17 85.1
Msa0712810 MtrunA17_Chr1g0164401 28.877 374 203 17 113 461 93 428 2.03e-17 84.7
Msa0712810 MtrunA17_Chr2g0310821 25.967 362 227 14 113 461 109 442 2.09e-17 84.7
Msa0712810 MtrunA17_Chr1g0210201 26.400 375 211 17 113 461 132 467 2.65e-17 84.7
Msa0712810 MtrunA17_Chr1g0159791 25.995 377 233 14 109 461 55 409 2.72e-17 84.3
Msa0712810 MtrunA17_Chr6g0484901 26.703 367 219 14 113 461 157 491 4.78e-17 84.0
Msa0712810 MtrunA17_Chr3g0078321 25.654 382 223 19 122 473 69 419 2.35e-16 81.3
Msa0712810 MtrunA17_Chr6g0487471 25.455 385 222 17 113 465 87 438 4.24e-16 80.5
Msa0712810 MtrunA17_Chr8g0390761 23.876 356 225 13 114 451 118 445 1.49e-15 79.0
Msa0712810 MtrunA17_Chr2g0333991 23.890 473 285 21 19 460 6 434 5.10e-15 77.4
Msa0712810 MtrunA17_Chr3g0099901 24.798 371 229 13 113 461 143 485 5.66e-15 77.4
Msa0712810 MtrunA17_Chr1g0165201 25.936 374 219 15 113 461 129 469 6.61e-15 77.0
Msa0712810 MtrunA17_Chr4g0023351 28.814 236 141 11 111 336 57 275 2.15e-14 73.9
Msa0712810 MtrunA17_Chr2g0312941 25.258 388 233 18 112 461 87 455 2.30e-14 75.5
Msa0712810 MtrunA17_Chr2g0311451 24.000 400 235 16 91 461 76 435 5.38e-14 74.3
Msa0712810 MtrunA17_Chr7g0267171 24.500 400 220 18 116 461 94 465 1.89e-13 72.8
Msa0712810 MtrunA17_Chr2g0291201 25.067 375 229 17 113 461 103 451 2.29e-13 72.4
Msa0712810 MtrunA17_Chr3g0099891 25.936 374 215 18 113 461 118 454 4.00e-12 68.6
Msa0712810 MtrunA17_Chr7g0239001 25.545 321 180 12 115 401 62 357 4.01e-12 68.2
Msa0712810 MtrunA17_Chr8g0352691 26.296 270 163 12 210 461 8 259 8.02e-11 62.8
Msa0712810 MtrunA17_Chr6g0486981 24.116 481 288 25 11 461 3 436 8.24e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0712810 AT2G17760.1 53.737 495 218 8 32 522 26 513 2.53e-176 507
Msa0712810 AT4G35880.1 50.881 454 211 9 17 465 9 455 5.58e-154 450
Msa0712810 AT4G35880.2 49.180 427 205 8 17 438 9 428 1.03e-136 403
Msa0712810 AT3G51330.1 48.874 444 211 12 31 465 21 457 1.17e-131 393
Msa0712810 AT3G51330.3 48.864 440 209 12 31 461 21 453 4.71e-130 387
Msa0712810 AT3G51340.2 46.053 456 229 12 16 465 7 451 8.96e-129 385
Msa0712810 AT3G51350.1 47.191 445 216 14 31 465 21 456 5.09e-128 384
Msa0712810 AT3G51360.1 44.612 464 236 12 24 481 7 455 1.46e-123 371
Msa0712810 AT3G51360.9 44.612 464 236 12 24 481 17 465 1.54e-123 371
Msa0712810 AT3G51340.1 44.421 466 234 14 16 465 7 463 1.67e-120 365
Msa0712810 AT3G51330.2 46.651 418 207 12 31 439 21 431 1.14e-112 342
Msa0712810 AT3G51360.10 43.632 424 218 12 23 440 6 414 5.85e-107 327
Msa0712810 AT3G51360.6 43.735 423 217 12 24 440 17 424 7.81e-107 327
Msa0712810 AT3G51340.3 44.059 404 210 11 16 414 7 399 2.61e-104 319
Msa0712810 AT3G51360.7 45.745 376 190 9 112 481 4 371 1.17e-102 315
Msa0712810 AT3G51360.4 43.990 391 202 10 24 412 7 382 3.02e-102 313
Msa0712810 AT3G51340.4 45.745 376 191 10 16 386 7 374 3.68e-102 313
Msa0712810 AT3G51360.5 43.990 391 202 10 24 412 17 392 4.86e-102 313
Msa0712810 AT3G51360.2 45.210 334 169 8 24 356 17 337 6.46e-91 282
Msa0712810 AT5G10080.1 35.950 484 272 14 22 487 8 471 2.27e-89 284
Msa0712810 AT3G51360.3 42.730 337 180 8 150 481 1 329 1.22e-79 254
Msa0712810 AT3G51360.8 42.730 337 180 8 150 481 1 329 1.22e-79 254
Msa0712810 AT3G02740.1 29.323 399 244 14 112 485 84 469 1.86e-35 139
Msa0712810 AT3G02740.2 29.323 399 244 14 112 485 9 394 1.93e-35 137
Msa0712810 AT5G36260.1 26.593 455 258 19 36 461 26 433 2.46e-31 127
Msa0712810 AT5G22850.1 27.176 471 292 19 55 500 29 473 5.12e-31 126
Msa0712810 AT1G05840.1 29.111 371 231 14 112 461 79 438 1.06e-30 125
Msa0712810 AT1G65240.1 27.807 374 226 14 112 461 73 426 3.40e-29 120
Msa0712810 AT4G33490.2 28.721 383 218 18 115 469 62 417 8.62e-29 119
Msa0712810 AT2G36670.2 27.427 412 264 16 72 461 59 457 1.59e-28 119
Msa0712810 AT2G36670.4 27.394 376 242 13 106 461 27 391 3.18e-28 117
Msa0712810 AT2G36670.3 27.394 376 242 13 106 461 27 391 3.18e-28 117
Msa0712810 AT2G36670.1 26.988 415 267 16 72 461 59 462 4.16e-28 118
Msa0712810 AT1G49050.2 28.463 397 213 21 109 461 26 395 4.52e-27 113
Msa0712810 AT1G49050.1 27.806 392 216 20 112 461 202 568 6.42e-26 112
Msa0712810 AT1G08210.1 26.411 443 265 19 112 526 83 492 1.77e-25 110
Msa0712810 AT1G08210.4 26.411 443 265 19 112 526 117 526 2.15e-25 110
Msa0712810 AT1G08210.3 26.411 443 265 19 112 526 131 540 2.55e-25 110
Msa0712810 AT1G44130.1 28.142 366 223 15 117 461 53 399 6.38e-25 107
Msa0712810 AT3G54400.1 26.146 371 218 14 113 461 88 424 3.60e-24 105
Msa0712810 AT4G33490.1 26.849 365 219 16 115 456 59 398 1.79e-23 103
Msa0712810 AT3G50050.1 26.854 391 224 20 119 481 99 455 3.55e-23 103
Msa0712810 AT1G08210.2 25.106 470 264 20 112 526 83 519 4.57e-22 100
Msa0712810 AT2G42980.1 27.132 387 226 15 113 463 160 526 1.77e-21 98.2
Msa0712810 AT3G61820.1 28.272 382 208 18 113 461 135 483 9.36e-21 95.9
Msa0712810 AT5G43100.2 25.591 465 273 24 69 504 37 457 1.09e-20 95.5
Msa0712810 AT5G43100.1 25.847 472 263 26 69 504 37 457 1.89e-20 95.5
Msa0712810 AT2G03200.1 29.288 379 205 19 116 461 110 458 3.45e-20 94.0
Msa0712810 AT1G49050.3 28.881 277 163 12 112 370 202 462 7.32e-20 92.8
Msa0712810 AT1G64830.1 28.836 378 205 19 113 461 86 428 7.32e-20 92.4
Msa0712810 AT3G25700.1 22.829 403 220 18 113 461 84 449 1.55e-19 91.7
Msa0712810 AT1G77480.2 25.000 376 239 16 109 461 64 419 3.87e-19 90.5
Msa0712810 AT1G77480.1 25.000 376 239 16 109 461 64 419 7.62e-19 89.7
Msa0712810 AT3G52500.1 24.627 402 222 13 115 461 92 467 1.51e-18 89.0
Msa0712810 AT5G33340.1 26.913 379 213 17 113 461 90 434 2.50e-18 87.8
Msa0712810 AT1G01300.1 26.330 376 217 15 113 461 142 484 2.55e-18 88.2
Msa0712810 AT4G30040.1 26.776 366 221 16 114 456 86 427 2.76e-18 87.8
Msa0712810 AT3G59080.1 26.302 384 231 14 113 463 170 534 3.73e-18 88.2
Msa0712810 AT5G10770.1 26.575 365 229 15 113 461 132 473 9.07e-18 86.7
Msa0712810 AT5G10760.1 27.671 365 216 14 113 461 132 464 9.46e-18 86.3
Msa0712810 AT2G23945.1 28.065 310 179 15 112 402 95 379 1.35e-17 85.9
Msa0712810 AT5G07030.1 25.474 369 214 17 119 461 121 454 2.16e-17 85.1
Msa0712810 AT3G18490.1 26.596 376 212 18 113 461 162 500 3.59e-16 81.6
Msa0712810 AT4G30030.1 25.538 372 222 15 114 461 79 419 5.84e-16 80.5
Msa0712810 AT1G25510.1 27.078 373 211 17 113 461 148 483 7.61e-16 80.5
Msa0712810 AT3G20015.1 24.889 450 256 18 62 461 53 470 1.53e-15 79.7
Msa0712810 AT1G09750.1 26.700 397 233 18 94 461 81 448 5.81e-15 77.8
Msa0712810 AT4G12920.1 24.793 363 232 15 110 461 55 387 1.13e-13 73.2
Msa0712810 AT1G31450.1 23.077 416 252 17 78 461 62 441 1.91e-13 72.8
Msa0712810 AT2G28040.2 24.119 369 221 18 111 461 54 381 1.13e-12 70.1
Msa0712810 AT3G42550.1 21.447 387 223 14 108 475 73 397 1.14e-12 70.5
Msa0712810 AT2G28040.1 24.119 369 221 18 111 461 63 390 1.48e-12 69.7
Msa0712810 AT5G45120.1 23.923 418 231 16 110 461 82 478 1.90e-12 70.1
Msa0712810 AT1G79720.1 26.700 397 214 19 103 461 125 482 2.12e-11 66.6
Msa0712810 AT5G02190.1 21.615 384 234 13 122 461 82 442 2.16e-11 66.6

Find 126 sgRNAs with CRISPR-Local

Find 248 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTACGATCTCTATTATATT+TGG 0.100965 5_1:-4609156 Msa0712810:three_prime_UTR
AATTTCCTCCCACAATAATT+TGG 0.127702 5_1:+4610702 None:intergenic
GAAGGGGTAGATTGTATTTC+AGG 0.187188 5_1:+4609464 None:intergenic
TGATCCGGTGAAGGGTATTT+TGG 0.196341 5_1:-4613380 Msa0712810:CDS
GATCATCATTATCTGTAATT+AGG 0.222151 5_1:+4611424 None:intergenic
GCTAACCCACTAATTACTTT+TGG 0.224798 5_1:-4611390 Msa0712810:CDS
CTATAATCTAGGGTTTAAAT+TGG 0.243602 5_1:+4609076 None:intergenic
TATCTTGTTGCATTAGATAC+TGG 0.267458 5_1:-4611933 Msa0712810:CDS
GATAGATATTCAACTTGATA+TGG 0.275532 5_1:+4611492 None:intergenic
ATCTGGTACGTGGTGTTATC+TGG 0.281632 5_1:+4613229 None:intergenic
CTGAAACATATAAAGAATTA+TGG 0.282426 5_1:+4613494 None:intergenic
TATACCACATAATTTATCTT+TGG 0.290660 5_1:-4609182 Msa0712810:three_prime_UTR
AAAATTTCGGAAAATATATA+TGG 0.291561 5_1:+4613602 None:intergenic
ACCCCGCCACTTGTTATTAT+CGG 0.293487 5_1:+4609991 None:intergenic
TACTTCAACTACTGGTTTCT+TGG 0.296582 5_1:-4611463 Msa0712810:CDS
CTTGATAGCATTGATTTGAT+AGG 0.319068 5_1:-4609241 Msa0712810:three_prime_UTR
CTCACATAAGCTGCTATTGC+AGG 0.328660 5_1:+4611554 None:intergenic
CATATAAAGAATTATGGATT+TGG 0.335778 5_1:+4613500 None:intergenic
ATACTGGGAGTGACTTGTTC+TGG 0.342280 5_1:-4611917 Msa0712810:CDS
GCAGCTCCAAATGGTCTGTT+TGG 0.347954 5_1:-4611120 Msa0712810:CDS
GTTTGGGAAGAATCACATTT+GGG 0.350381 5_1:-4611008 Msa0712810:CDS
TGGTGAGAGATTATAGTTTA+CGG 0.356510 5_1:+4609096 None:intergenic
CCTTGTTTGGCTAAGATACT+TGG 0.362835 5_1:+4611072 None:intergenic
TTCTTAAATGAGTGACTAGA+TGG 0.366521 5_1:+4609422 None:intergenic
TGTTAACACTGTTCTTTGTT+TGG 0.367503 5_1:-4609869 Msa0712810:CDS
ATTTCCTCCCACAATAATTT+GGG 0.367517 5_1:+4610703 None:intergenic
CATTGGGAACTTCAATTGTC+TGG 0.371141 5_1:+4610058 None:intergenic
AGAACCTGCATTAGCATTTA+CGG 0.372839 5_1:-4609399 Msa0712810:CDS
ACGTACCAGATTAGTTTGTT+TGG 0.379494 5_1:-4613217 Msa0712810:CDS
TTGAGTACTGCTATGACTTA+AGG 0.381947 5_1:-4610434 Msa0712810:intron
ATTTGAAGTTAGCCCTTGTT+TGG 0.382291 5_1:+4611059 None:intergenic
AGCAACAATGTGAATATCAT+TGG 0.391322 5_1:-4609835 Msa0712810:CDS
GGTTTGGGAAGAATCACATT+TGG 0.393676 5_1:-4611009 Msa0712810:CDS
GGGGCGTGCGATCGGTTAAC+AGG 0.398760 5_1:+4609515 None:intergenic
AGTTGTGGGCAAGTTCAAAC+AGG 0.401886 5_1:-4611159 Msa0712810:CDS
GGGAGGAAATTCCGCTGATC+TGG 0.403580 5_1:-4610690 Msa0712810:CDS
CCTAGTCCAAACAGACCATT+TGG 0.407470 5_1:+4611114 None:intergenic
ACTATCCAAACAAACTAATC+TGG 0.410497 5_1:+4613212 None:intergenic
CTCTATACCATGGACACATT+TGG 0.418622 5_1:+4611878 None:intergenic
TTCAATGCAATATTCGATAC+TGG 0.419948 5_1:-4610665 Msa0712810:CDS
CTGGATGGGGCAGCTCCAAA+TGG 0.437755 5_1:-4611129 Msa0712810:CDS
TGACTAGATGGAAGTCTTTG+AGG 0.440995 5_1:+4609434 None:intergenic
AACTACTGGTTTCTTGGTAG+AGG 0.441070 5_1:-4611457 Msa0712810:CDS
TTCATCGTCAAGTTAACATT+GGG 0.443905 5_1:+4610042 None:intergenic
ACACAGTGCTCTTCTTCATC+TGG 0.446019 5_1:-4611525 Msa0712810:CDS
TATTCACGACTTCCCTTGTC+AGG 0.449376 5_1:+4613343 None:intergenic
TGCCACGGTTCTAGTTCATT+CGG 0.451749 5_1:-4613427 Msa0712810:CDS
TAGTTTACGGATTAAATTGA+CGG 0.451993 5_1:+4609109 None:intergenic
CCCCGCCACTTGTTATTATC+GGG 0.460471 5_1:+4609992 None:intergenic
TTGATAGCATTGATTTGATA+GGG 0.465898 5_1:-4609240 Msa0712810:three_prime_UTR
CCAAATGGTCTGTTTGGACT+AGG 0.469915 5_1:-4611114 Msa0712810:CDS
TGAGTAATTTGCTTATAAGC+CGG 0.474988 5_1:+4610617 None:intergenic
CCCAGCTGTGTCTCCTGCTA+TGG 0.476244 5_1:-4609495 Msa0712810:CDS
CGGGGTCGGCGTCTTGCTGA+CGG 0.476845 5_1:-4613286 Msa0712810:CDS
TCAGGATTCACCGCCATAGC+AGG 0.482898 5_1:+4609482 None:intergenic
AGTTACCCAAAAGTAATTAG+TGG 0.486929 5_1:+4611384 None:intergenic
TCAGAAAATACTTCAACTAC+TGG 0.487753 5_1:-4611471 Msa0712810:CDS
TTTCATCGTCAAGTTAACAT+TGG 0.489542 5_1:+4610041 None:intergenic
ATTATGGATTTGGTAGGAGA+TGG 0.490259 5_1:+4613510 None:intergenic
CTCATCATATTCATTCATCA+TGG 0.493531 5_1:-4613542 Msa0712810:exon
AGTCTTTGAGGACCATTTGA+AGG 0.496392 5_1:+4609446 None:intergenic
GAGTAATTTGCTTATAAGCC+GGG 0.497835 5_1:+4610618 None:intergenic
ATACAATCTACCCCTTCAAA+TGG 0.498025 5_1:-4609458 Msa0712810:CDS
ATCTTGTTGCATTAGATACT+GGG 0.501285 5_1:-4611932 Msa0712810:CDS
GCCATAGCAGGAGACACAGC+TGG 0.503097 5_1:+4609494 None:intergenic
TATGCAGAAAACTTCATGAC+TGG 0.503906 5_1:-4609723 Msa0712810:intron
CATGGTATAGAGCTATCAAC+AGG 0.505796 5_1:-4611867 Msa0712810:CDS
CACTGTCACCCAAATTATTG+TGG 0.506380 5_1:-4610711 Msa0712810:CDS
AATGTTAACTTGACGATGAA+AGG 0.513228 5_1:-4610039 Msa0712810:CDS
CCATAGCAGGAGACACAGCT+GGG 0.513250 5_1:+4609495 None:intergenic
CCAAGTATCTTAGCCAAACA+AGG 0.519276 5_1:-4611072 Msa0712810:CDS
TGGGGATAATAATAGTAGCT+TGG 0.520297 5_1:-4610989 Msa0712810:CDS
AAGTCATAGCAGTACTCAAA+GGG 0.522967 5_1:+4610438 None:intergenic
GTTGTTTAAGTATGTAAATG+AGG 0.527623 5_1:+4610640 None:intergenic
ACACAGCTGGGGCGTGCGAT+CGG 0.529343 5_1:+4609507 None:intergenic
ACCCGATAATAACAAGTGGC+GGG 0.529981 5_1:-4609993 Msa0712810:CDS
GTTTGATTTCACAATCACAT+AGG 0.531519 5_1:-4613450 Msa0712810:CDS
GTCTTTGAGGACCATTTGAA+GGG 0.534896 5_1:+4609447 None:intergenic
GTTACCCAAAAGTAATTAGT+GGG 0.541649 5_1:+4611385 None:intergenic
TAAAGAATTATGGATTTGGT+AGG 0.543345 5_1:+4613504 None:intergenic
GCGAGAACATGACTCTAGGT+TGG 0.557604 5_1:-4609680 Msa0712810:CDS
ACTGTCACCCAAATTATTGT+GGG 0.559749 5_1:-4610710 Msa0712810:CDS
GATCGCGAGAACATGACTCT+AGG 0.567796 5_1:-4609684 Msa0712810:CDS
TATTGAGAACGTTCCTGACA+AGG 0.569108 5_1:-4613356 Msa0712810:CDS
TCGTGAATACTATGTTGCTA+TGG 0.570427 5_1:-4613329 Msa0712810:CDS
GAATCCAAAGATAAATTATG+TGG 0.573689 5_1:+4609178 None:intergenic
GCCACCGTAAATGCTAATGC+AGG 0.574848 5_1:+4609395 None:intergenic
TGCGACGGCCTAATATTGAA+TGG 0.577927 5_1:+4610952 None:intergenic
CACATTTGGTACAATTGCAA+GGG 0.578522 5_1:+4611892 None:intergenic
ACACATTTGGTACAATTGCA+AGG 0.579567 5_1:+4611891 None:intergenic
ATACCCAAAATACCCTTCAC+CGG 0.579612 5_1:+4613376 None:intergenic
GACCCGATAATAACAAGTGG+CGG 0.582074 5_1:-4609994 Msa0712810:CDS
GCAACAAGATACGATGAAGC+TGG 0.584329 5_1:+4611945 None:intergenic
CAGTGCTCTTCTTCATCTGG+TGG 0.585118 5_1:-4611522 Msa0712810:CDS
TAAGTCATAGCAGTACTCAA+AGG 0.585485 5_1:+4610437 None:intergenic
AAAACTTCATGACTGGCTAC+CGG 0.586042 5_1:-4609716 Msa0712810:CDS
AGCTGTGTCTCCTGCTATGG+CGG 0.589379 5_1:-4609492 Msa0712810:CDS
GAACATGACTCTAGGTTGGA+AGG 0.591059 5_1:-4609676 Msa0712810:CDS
ACAATAACCACTTACTGCGA+CGG 0.595509 5_1:+4610937 None:intergenic
TGGTGTTCCAACAGAAACAT+TGG 0.596649 5_1:+4611965 None:intergenic
CCGGATCAGAAAACCGATGG+TGG 0.596699 5_1:+4613395 None:intergenic
GCGTGTGAAGTAAATGTCAA+TGG 0.600629 5_1:+4613664 None:intergenic
CCCGATAATAACAAGTGGCG+GGG 0.603495 5_1:-4609992 Msa0712810:intron
TATAGAGCTATCAACAGGAC+AGG 0.606470 5_1:-4611862 Msa0712810:intron
AATAATAGTAGCTTGGACCA+AGG 0.612712 5_1:-4610982 Msa0712810:CDS
TCGGTTTCGATATCCACCAT+CGG 0.616803 5_1:-4613408 Msa0712810:CDS
GTTAACTTGACGATGAAAGG+TGG 0.618692 5_1:-4610036 Msa0712810:CDS
TCACAATCACATAGGTGCCA+CGG 0.624975 5_1:-4613442 Msa0712810:CDS
AATTGTACCAAATGTGTCCA+TGG 0.625959 5_1:-4611885 Msa0712810:CDS
TCACCGGATCAGAAAACCGA+TGG 0.631331 5_1:+4613392 None:intergenic
GTCACCCAAATTATTGTGGG+AGG 0.641019 5_1:-4610707 Msa0712810:CDS
ATTGAGAACGTTCCTGACAA+GGG 0.643964 5_1:-4613355 Msa0712810:CDS
ATTTACATACTTAAACAACC+CGG 0.646591 5_1:-4610636 Msa0712810:CDS
TGCATTGAACTCCAGATCAG+CGG 0.650143 5_1:+4610679 None:intergenic
AACCGAATGAACTAGAACCG+TGG 0.654040 5_1:+4613425 None:intergenic
TTTGGGAAGAATCACATTTG+GGG 0.655444 5_1:-4611007 Msa0712810:CDS
GGTCGGCGTCTTGCTGACGG+TGG 0.660969 5_1:-4613283 Msa0712810:CDS
CTGTTGATAGCTCTATACCA+TGG 0.663638 5_1:+4611868 None:intergenic
GTTGTGGGCAAGTTCAAACA+GGG 0.666836 5_1:-4611158 Msa0712810:CDS
ATGGACCCGATAATAACAAG+TGG 0.682007 5_1:-4609997 Msa0712810:CDS
CATAGCAGGAGACACAGCTG+GGG 0.686306 5_1:+4609496 None:intergenic
ACCTGCATTAGCATTTACGG+TGG 0.694138 5_1:-4609396 Msa0712810:CDS
TCTTTGAGGACCATTTGAAG+GGG 0.697645 5_1:+4609448 None:intergenic
CAAGTATCTTAGCCAAACAA+GGG 0.698265 5_1:-4611071 Msa0712810:CDS
TTGTGGGCAAGTTCAAACAG+GGG 0.708328 5_1:-4611157 Msa0712810:CDS
AAACAAACTAATCTGGTACG+TGG 0.721432 5_1:+4613219 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAGAAATAATGTAATTAA+AGG + chr5_1:4609821-4609840 None:intergenic 10.0%
!!! CAAAATTCATTTTTTTTTAA+TGG - chr5_1:4610560-4610579 Msa0712810:intron 10.0%
!! AAAATGAAAAGAATAATTAA+GGG + chr5_1:4610606-4610625 None:intergenic 10.0%
!! AAAAATGAAAAGAATAATTA+AGG + chr5_1:4610607-4610626 None:intergenic 10.0%
!!! TAATTATTCTTTTCATTTTT+TGG - chr5_1:4610607-4610626 Msa0712810:intron 10.0%
!!! AATTATTCTTTTCATTTTTT+GGG - chr5_1:4610608-4610627 Msa0712810:intron 10.0%
!! AAAATATTGTATAGTTAAAT+CGG + chr5_1:4611544-4611563 None:intergenic 10.0%
!!! TTAACTATACAATATTTTTT+TGG - chr5_1:4611546-4611565 Msa0712810:CDS 10.0%
!!! ATAATTTTTAAATATGTAAC+TGG - chr5_1:4612208-4612227 Msa0712810:intron 10.0%
!! AAAATTTCGGAAAATATATA+TGG + chr5_1:4609181-4609200 None:intergenic 15.0%
!! TATAACAAAAAATTAAGTAG+TGG + chr5_1:4609608-4609627 None:intergenic 15.0%
!!! TTTTAATTTCATCTTTTTTC+TGG - chr5_1:4609628-4609647 Msa0712810:intron 15.0%
!!! TTTTTAGAAAGAAAAAGATT+GGG - chr5_1:4609743-4609762 Msa0712810:intron 15.0%
!!! TTTCCATTTTAGATAATTTT+TGG - chr5_1:4609838-4609857 Msa0712810:CDS 15.0%
!! ATTCCAAAAATTATCTAAAA+TGG + chr5_1:4609844-4609863 None:intergenic 15.0%
!!! TTTGGAATTGAATATTTAAA+AGG - chr5_1:4609856-4609875 Msa0712810:CDS 15.0%
!! AACATTAATCTCTTAAATTT+AGG + chr5_1:4610135-4610154 None:intergenic 15.0%
!! ATCTAAAAAAAAATCTGATA+TGG - chr5_1:4610416-4610435 Msa0712810:intron 15.0%
!!! GTTACTAAACATTTTTTTTT+TGG + chr5_1:4611480-4611499 None:intergenic 15.0%
!! ATTCAAAAATTAAACTACAA+AGG - chr5_1:4612292-4612311 Msa0712810:intron 15.0%
!! AATTATGTTATGTTATTATC+AGG - chr5_1:4613217-4613236 Msa0712810:CDS 15.0%
!! CATATAAAGAATTATGGATT+TGG + chr5_1:4609283-4609302 None:intergenic 20.0%
!! CTGAAACATATAAAGAATTA+TGG + chr5_1:4609289-4609308 None:intergenic 20.0%
!!! CTTTTTAGAAAGAAAAAGAT+TGG - chr5_1:4609742-4609761 Msa0712810:intron 20.0%
!!! ACTTTTTCTTTGTAAATCTA+AGG + chr5_1:4609998-4610017 None:intergenic 20.0%
!! GAGAGAAATTTATCATAAAA+TGG - chr5_1:4610078-4610097 Msa0712810:intron 20.0%
!! AAAAAATAATCCATAAGTCT+TGG - chr5_1:4610245-4610264 Msa0712810:intron 20.0%
!!! TTCACATTTAGATTCTTTTT+TGG + chr5_1:4610447-4610466 None:intergenic 20.0%
!!! ATTCATTTTTTTTTAATGGG+AGG - chr5_1:4610564-4610583 Msa0712810:intron 20.0%
!! ATGAATCTAAAAATACTTGA+TGG - chr5_1:4610680-4610699 Msa0712810:CDS 20.0%
!! TGAATCTAAAAATACTTGAT+GGG - chr5_1:4610681-4610700 Msa0712810:CDS 20.0%
!!! TGGTTTTGATACATTTTTTT+TGG + chr5_1:4611068-4611087 None:intergenic 20.0%
!! TACAAGATTAAACTATAGTA+TGG + chr5_1:4611088-4611107 None:intergenic 20.0%
!!! AATTCTTTTTCAATGTGTTT+TGG - chr5_1:4611741-4611760 Msa0712810:intron 20.0%
!! TTAATTAATAAGAACTCAAG+TGG + chr5_1:4612248-4612267 None:intergenic 20.0%
!! AATAACTAATGGAGAATTAA+AGG + chr5_1:4613187-4613206 None:intergenic 20.0%
!! TTCTCATTAACAATAACTAA+TGG + chr5_1:4613198-4613217 None:intergenic 20.0%
!! CTAAAAAAATTGATAAAAGC+AGG + chr5_1:4613417-4613436 None:intergenic 20.0%
!!! ATTACTATACGTTTTTTTTC+TGG - chr5_1:4613513-4613532 Msa0712810:CDS 20.0%
!! TATACCACATAATTTATCTT+TGG - chr5_1:4613598-4613617 Msa0712810:five_prime_UTR 20.0%
! CGTGTGATATGAAAAAATTT+CGG + chr5_1:4609194-4609213 None:intergenic 25.0%
!! TAAAGAATTATGGATTTGGT+AGG + chr5_1:4609279-4609298 None:intergenic 25.0%
! ATAAAGTTCATAGAATCACA+CGG - chr5_1:4609712-4609731 Msa0712810:CDS 25.0%
! GTAATTAAAGGCTAAAGATT+TGG + chr5_1:4609809-4609828 None:intergenic 25.0%
! TATACTACACTAAATTGTAC+AGG - chr5_1:4609942-4609961 Msa0712810:intron 25.0%
!!! CATTTAGATTCTTTTTTGGA+AGG + chr5_1:4610443-4610462 None:intergenic 25.0%
! ATCAAGAATGCATAACAAAA+AGG + chr5_1:4610712-4610731 None:intergenic 25.0%
! GATAGATATTCAACTTGATA+TGG + chr5_1:4611291-4611310 None:intergenic 25.0%
! GATCATCATTATCTGTAATT+AGG + chr5_1:4611359-4611378 None:intergenic 25.0%
!!! TTTTTTGGACTTGCTTTTTT+TGG - chr5_1:4611561-4611580 Msa0712810:CDS 25.0%
! GAAAAACACCATTCAATATT+AGG - chr5_1:4611820-4611839 Msa0712810:intron 25.0%
! CTTTAAACTATCTTTCTGAA+AGG - chr5_1:4611976-4611995 Msa0712810:CDS 25.0%
! GTTGTTTAAGTATGTAAATG+AGG + chr5_1:4612143-4612162 None:intergenic 25.0%
! ATTTACATACTTAAACAACC+CGG - chr5_1:4612144-4612163 Msa0712810:intron 25.0%
! TTGTTAAGTTAGTTACAAGT+TGG - chr5_1:4612475-4612494 Msa0712810:intron 25.0%
!! GAGCATGAAATATAACTTTT+TGG - chr5_1:4612814-4612833 Msa0712810:intron 25.0%
! AACCTTATAAATGATTCATG+TGG - chr5_1:4612874-4612893 Msa0712810:intron 25.0%
! TACCACATGAATCATTTATA+AGG + chr5_1:4612879-4612898 None:intergenic 25.0%
!! TTTGAAGTACAAACTACAAA+GGG + chr5_1:4613153-4613172 None:intergenic 25.0%
!!! TTTTGAAGTACAAACTACAA+AGG + chr5_1:4613154-4613173 None:intergenic 25.0%
!!! TTGATAGCATTGATTTGATA+GGG - chr5_1:4613540-4613559 Msa0712810:exon 25.0%
! GAATCCAAAGATAAATTATG+TGG + chr5_1:4613605-4613624 None:intergenic 25.0%
! CTTACGATCTCTATTATATT+TGG - chr5_1:4613624-4613643 Msa0712810:five_prime_UTR 25.0%
!! TAGTTTACGGATTAAATTGA+CGG + chr5_1:4613674-4613693 None:intergenic 25.0%
CTCATCATATTCATTCATCA+TGG - chr5_1:4609238-4609257 Msa0712810:three_prime_UTR 30.0%
! GTTTGATTTCACAATCACAT+AGG - chr5_1:4609330-4609349 Msa0712810:three_prime_UTR 30.0%
ACTATCCAAACAAACTAATC+TGG + chr5_1:4609571-4609590 None:intergenic 30.0%
TAAAGTTCATAGAATCACAC+GGG - chr5_1:4609713-4609732 Msa0712810:CDS 30.0%
AATTGCTATGTTTGAACACT+TGG - chr5_1:4610289-4610308 Msa0712810:intron 30.0%
TTGAAAATCACACACACAAA+TGG + chr5_1:4610331-4610350 None:intergenic 30.0%
!!! TCTTTTCATTTTTTGGGTCA+TGG - chr5_1:4610614-4610633 Msa0712810:CDS 30.0%
! TTTGTTATGCATTCTTGATG+TGG - chr5_1:4610713-4610732 Msa0712810:CDS 30.0%
!!! CATTGCTTATTTTTTCCTCT+TGG - chr5_1:4610769-4610788 Msa0712810:intron 30.0%
AAACTGAAATACAAACCAAG+AGG + chr5_1:4610787-4610806 None:intergenic 30.0%
TATCTTGTTGCATTAGATAC+TGG - chr5_1:4610847-4610866 Msa0712810:intron 30.0%
ATCTTGTTGCATTAGATACT+GGG - chr5_1:4610848-4610867 Msa0712810:intron 30.0%
!!! TTTTTTTTGGCACACAACAA+AGG + chr5_1:4611055-4611074 None:intergenic 30.0%
TCAGAAAATACTTCAACTAC+TGG - chr5_1:4611309-4611328 Msa0712810:intron 30.0%
!! CTAACCCACTAATTACTTTT+GGG - chr5_1:4611391-4611410 Msa0712810:CDS 30.0%
GTTACCCAAAAGTAATTAGT+GGG + chr5_1:4611398-4611417 None:intergenic 30.0%
AGTTACCCAAAAGTAATTAG+TGG + chr5_1:4611399-4611418 None:intergenic 30.0%
! TATTGAATGGTGTTTTTCCT+TGG + chr5_1:4611818-4611837 None:intergenic 30.0%
!!! CATATATGTTTTTAGCAGCT+TGG - chr5_1:4611887-4611906 Msa0712810:CDS 30.0%
!! TAGACCTTTGAGGTTAATTT+GGG + chr5_1:4611955-4611974 None:intergenic 30.0%
!! ATAGACCTTTGAGGTTAATT+TGG + chr5_1:4611956-4611975 None:intergenic 30.0%
AGTTTAAAGATAGACCTTTG+AGG + chr5_1:4611965-4611984 None:intergenic 30.0%
ATTTCCTCCCACAATAATTT+GGG + chr5_1:4612080-4612099 None:intergenic 30.0%
AATTTCCTCCCACAATAATT+TGG + chr5_1:4612081-4612100 None:intergenic 30.0%
TTCAATGCAATATTCGATAC+TGG - chr5_1:4612115-4612134 Msa0712810:intron 30.0%
!! TGAGTAATTTGCTTATAAGC+CGG + chr5_1:4612166-4612185 None:intergenic 30.0%
TAATAAGAACTCAAGTGGAA+TGG + chr5_1:4612243-4612262 None:intergenic 30.0%
GAATCAAACTGCAAAAACAA+TGG + chr5_1:4612276-4612295 None:intergenic 30.0%
TTCATCGTCAAGTTAACATT+GGG + chr5_1:4612741-4612760 None:intergenic 30.0%
TTTCATCGTCAAGTTAACAT+TGG + chr5_1:4612742-4612761 None:intergenic 30.0%
! AATGTTAACTTGACGATGAA+AGG - chr5_1:4612741-4612760 Msa0712810:intron 30.0%
!! TGGAGACAATTATTTTGTCA+TGG - chr5_1:4612764-4612783 Msa0712810:intron 30.0%
TGTTAACACTGTTCTTTGTT+TGG - chr5_1:4612911-4612930 Msa0712810:intron 30.0%
AGCAACAATGTGAATATCAT+TGG - chr5_1:4612945-4612964 Msa0712810:intron 30.0%
TTCTTAAATGAGTGACTAGA+TGG + chr5_1:4613361-4613380 None:intergenic 30.0%
!! CTTGATAGCATTGATTTGAT+AGG - chr5_1:4613539-4613558 Msa0712810:exon 30.0%
TGGTGAGAGATTATAGTTTA+CGG + chr5_1:4613687-4613706 None:intergenic 30.0%
! ATTATGGATTTGGTAGGAGA+TGG + chr5_1:4609273-4609292 None:intergenic 35.0%
TCGTGAATACTATGTTGCTA+TGG - chr5_1:4609451-4609470 Msa0712810:CDS 35.0%
AAACAAACTAATCTGGTACG+TGG + chr5_1:4609564-4609583 None:intergenic 35.0%
!! ACGTACCAGATTAGTTTGTT+TGG - chr5_1:4609563-4609582 Msa0712810:intron 35.0%
CAACCACATATGTTTGTGTT+TGG - chr5_1:4609682-4609701 Msa0712810:CDS 35.0%
GTACCAAACACAAACATATG+TGG + chr5_1:4609688-4609707 None:intergenic 35.0%
AAAATCACACACACAAATGG+AGG + chr5_1:4610328-4610347 None:intergenic 35.0%
!! CATTTTGCCAATGTTTCTGT+TGG - chr5_1:4610808-4610827 Msa0712810:intron 35.0%
CACATTTGGTACAATTGCAA+GGG + chr5_1:4610891-4610910 None:intergenic 35.0%
ACACATTTGGTACAATTGCA+AGG + chr5_1:4610892-4610911 None:intergenic 35.0%
AATTGTACCAAATGTGTCCA+TGG - chr5_1:4610895-4610914 Msa0712810:intron 35.0%
CAAAACTATACTGCAGAGAT+TGG - chr5_1:4610982-4611001 Msa0712810:CDS 35.0%
ACATCTTAATACCCCTACTT+AGG + chr5_1:4611024-4611043 None:intergenic 35.0%
!! AAGTAGGGGTATTAAGATGT+CGG - chr5_1:4611024-4611043 Msa0712810:CDS 35.0%
!! TACTTCAACTACTGGTTTCT+TGG - chr5_1:4611317-4611336 Msa0712810:intron 35.0%
! GCTAACCCACTAATTACTTT+TGG - chr5_1:4611390-4611409 Msa0712810:CDS 35.0%
TTCCTCGCTAAGTTTAGTAA+TGG - chr5_1:4611445-4611464 Msa0712810:CDS 35.0%
TTCCATTACTAAACTTAGCG+AGG + chr5_1:4611450-4611469 None:intergenic 35.0%
ATTGAATGTGTTGCAGTTGT+GGG - chr5_1:4611607-4611626 Msa0712810:CDS 35.0%
CAAGTATCTTAGCCAAACAA+GGG - chr5_1:4611709-4611728 Msa0712810:intron 35.0%
! ATTTGAAGTTAGCCCTTGTT+TGG + chr5_1:4611724-4611743 None:intergenic 35.0%
!!! TCAATGTGTTTTGGTGATGA+TGG - chr5_1:4611750-4611769 Msa0712810:intron 35.0%
!!! TGTTTTGGTGATGATGGTTT+GGG - chr5_1:4611756-4611775 Msa0712810:intron 35.0%
GTTTGGGAAGAATCACATTT+GGG - chr5_1:4611772-4611791 Msa0712810:intron 35.0%
TTTGGGAAGAATCACATTTG+GGG - chr5_1:4611773-4611792 Msa0712810:intron 35.0%
TGGGGATAATAATAGTAGCT+TGG - chr5_1:4611791-4611810 Msa0712810:intron 35.0%
AATAATAGTAGCTTGGACCA+AGG - chr5_1:4611798-4611817 Msa0712810:intron 35.0%
TAGCAGCTTGGATAATGTTA+AGG - chr5_1:4611899-4611918 Msa0712810:CDS 35.0%
ACTGTCACCCAAATTATTGT+GGG - chr5_1:4612070-4612089 Msa0712810:intron 35.0%
!! GAGTAATTTGCTTATAAGCC+GGG + chr5_1:4612165-4612184 None:intergenic 35.0%
AAGTCATAGCAGTACTCAAA+GGG + chr5_1:4612345-4612364 None:intergenic 35.0%
TAAGTCATAGCAGTACTCAA+AGG + chr5_1:4612346-4612365 None:intergenic 35.0%
! TTGAGTACTGCTATGACTTA+AGG - chr5_1:4612346-4612365 Msa0712810:intron 35.0%
TATATCCAACAATCAGTGCT+TGG + chr5_1:4612427-4612446 None:intergenic 35.0%
! CACTGATTGTTGGATATAGT+TGG - chr5_1:4612429-4612448 Msa0712810:intron 35.0%
ATTCATGTGGTATTCATTCC+AGG - chr5_1:4612887-4612906 Msa0712810:intron 35.0%
TTCATGTGGTATTCATTCCA+GGG - chr5_1:4612888-4612907 Msa0712810:intron 35.0%
TATGCAGAAAACTTCATGAC+TGG - chr5_1:4613057-4613076 Msa0712810:intron 35.0%
ATACAATCTACCCCTTCAAA+TGG - chr5_1:4613322-4613341 Msa0712810:CDS 35.0%
AGAACCTGCATTAGCATTTA+CGG - chr5_1:4613381-4613400 Msa0712810:CDS 35.0%
GCGTGTGAAGTAAATGTCAA+TGG + chr5_1:4609119-4609138 None:intergenic 40.0%
ATACCCAAAATACCCTTCAC+CGG + chr5_1:4609407-4609426 None:intergenic 40.0%
TATTGAGAACGTTCCTGACA+AGG - chr5_1:4609424-4609443 Msa0712810:CDS 40.0%
ATTGAGAACGTTCCTGACAA+GGG - chr5_1:4609425-4609444 Msa0712810:CDS 40.0%
TGGTGTTCCAACAGAAACAT+TGG + chr5_1:4610818-4610837 None:intergenic 40.0%
CTCTATACCATGGACACATT+TGG + chr5_1:4610905-4610924 None:intergenic 40.0%
!! CTGTTGATAGCTCTATACCA+TGG + chr5_1:4610915-4610934 None:intergenic 40.0%
CATGGTATAGAGCTATCAAC+AGG - chr5_1:4610913-4610932 Msa0712810:intron 40.0%
TATAGAGCTATCAACAGGAC+AGG - chr5_1:4610918-4610937 Msa0712810:intron 40.0%
!! AACTACTGGTTTCTTGGTAG+AGG - chr5_1:4611323-4611342 Msa0712810:intron 40.0%
CATTGAATGTGTTGCAGTTG+TGG - chr5_1:4611606-4611625 Msa0712810:CDS 40.0%
! CCTTGTTTGGCTAAGATACT+TGG + chr5_1:4611711-4611730 None:intergenic 40.0%
CCAAGTATCTTAGCCAAACA+AGG - chr5_1:4611708-4611727 Msa0712810:intron 40.0%
!!! GTGTTTTGGTGATGATGGTT+TGG - chr5_1:4611755-4611774 Msa0712810:intron 40.0%
GGTTTGGGAAGAATCACATT+TGG - chr5_1:4611771-4611790 Msa0712810:intron 40.0%
ACAATAACCACTTACTGCGA+CGG + chr5_1:4611846-4611865 None:intergenic 40.0%
CACTGTCACCCAAATTATTG+TGG - chr5_1:4612069-4612088 Msa0712810:intron 40.0%
!! AATCACCAAGCACTGATTGT+TGG - chr5_1:4612419-4612438 Msa0712810:intron 40.0%
CTGATACACTTTACAGTTGG+TGG + chr5_1:4612596-4612615 None:intergenic 40.0%
CAGCTGATACACTTTACAGT+TGG + chr5_1:4612599-4612618 None:intergenic 40.0%
CTGATACACTTTACAGTTGG+TGG + chr5_1:4612596-4612615 None:intergenic 40.0%
CAGCTGATACACTTTACAGT+TGG + chr5_1:4612599-4612618 None:intergenic 40.0%
CATTGGGAACTTCAATTGTC+TGG + chr5_1:4612725-4612744 None:intergenic 40.0%
! GTTAACTTGACGATGAAAGG+TGG - chr5_1:4612744-4612763 Msa0712810:intron 40.0%
ATGGACCCGATAATAACAAG+TGG - chr5_1:4612783-4612802 Msa0712810:intron 40.0%
AAAGAACAGTGTTAACACCC+TGG + chr5_1:4612908-4612927 None:intergenic 40.0%
! AAAACTTCATGACTGGCTAC+CGG - chr5_1:4613064-4613083 Msa0712810:intron 40.0%
TCTCGCGATCAAAAACTATC+CGG + chr5_1:4613086-4613105 None:intergenic 40.0%
GAAGGGGTAGATTGTATTTC+AGG + chr5_1:4613319-4613338 None:intergenic 40.0%
! TCTTTGAGGACCATTTGAAG+GGG + chr5_1:4613335-4613354 None:intergenic 40.0%
! GTCTTTGAGGACCATTTGAA+GGG + chr5_1:4613336-4613355 None:intergenic 40.0%
! AGTCTTTGAGGACCATTTGA+AGG + chr5_1:4613337-4613356 None:intergenic 40.0%
TGACTAGATGGAAGTCTTTG+AGG + chr5_1:4613349-4613368 None:intergenic 40.0%
TCACAATCACATAGGTGCCA+CGG - chr5_1:4609338-4609357 Msa0712810:three_prime_UTR 45.0%
!! TGCCACGGTTCTAGTTCATT+CGG - chr5_1:4609353-4609372 Msa0712810:exon 45.0%
AACCGAATGAACTAGAACCG+TGG + chr5_1:4609358-4609377 None:intergenic 45.0%
TCGGTTTCGATATCCACCAT+CGG - chr5_1:4609372-4609391 Msa0712810:CDS 45.0%
!!! TGATCCGGTGAAGGGTATTT+TGG - chr5_1:4609400-4609419 Msa0712810:CDS 45.0%
!!! GATCCGGTGAAGGGTATTTT+GGG - chr5_1:4609401-4609420 Msa0712810:CDS 45.0%
TATTCACGACTTCCCTTGTC+AGG + chr5_1:4609440-4609459 None:intergenic 45.0%
!! ATCTGGTACGTGGTGTTATC+TGG + chr5_1:4609554-4609573 None:intergenic 45.0%
GCTGATTGAGCCAAGACTTA+TGG + chr5_1:4610258-4610277 None:intergenic 45.0%
CACACACAAATGGAGGAGTT+GGG + chr5_1:4610321-4610340 None:intergenic 45.0%
ACACACACAAATGGAGGAGT+TGG + chr5_1:4610322-4610341 None:intergenic 45.0%
GCAACAAGATACGATGAAGC+TGG + chr5_1:4610838-4610857 None:intergenic 45.0%
!! ATACTGGGAGTGACTTGTTC+TGG - chr5_1:4610863-4610882 Msa0712810:intron 45.0%
GCAAGCTTGTGACCTAAGTA+GGG - chr5_1:4611009-4611028 Msa0712810:CDS 45.0%
CAAGCTTGTGACCTAAGTAG+GGG - chr5_1:4611010-4611029 Msa0712810:CDS 45.0%
!! AGGGGTATTAAGATGTCGGA+AGG - chr5_1:4611028-4611047 Msa0712810:CDS 45.0%
! GCAGGCAACATTTTTGCTAG+TGG + chr5_1:4611211-4611230 None:intergenic 45.0%
CTCACATAAGCTGCTATTGC+AGG + chr5_1:4611229-4611248 None:intergenic 45.0%
ACACAGTGCTCTTCTTCATC+TGG - chr5_1:4611255-4611274 Msa0712810:intron 45.0%
AGTTGTGGGCAAGTTCAAAC+AGG - chr5_1:4611621-4611640 Msa0712810:intron 45.0%
GTTGTGGGCAAGTTCAAACA+GGG - chr5_1:4611622-4611641 Msa0712810:intron 45.0%
TTGTGGGCAAGTTCAAACAG+GGG - chr5_1:4611623-4611642 Msa0712810:intron 45.0%
! AGTTCAAACAGGGGCTTTTC+TGG - chr5_1:4611632-4611651 Msa0712810:intron 45.0%
! AAACAGGGGCTTTTCTGGAT+GGG - chr5_1:4611637-4611656 Msa0712810:intron 45.0%
CCTAGTCCAAACAGACCATT+TGG + chr5_1:4611669-4611688 None:intergenic 45.0%
CCAAATGGTCTGTTTGGACT+AGG - chr5_1:4611666-4611685 Msa0712810:intron 45.0%
TGCGACGGCCTAATATTGAA+TGG + chr5_1:4611831-4611850 None:intergenic 45.0%
!! CCTTTGAGGTTAATTTGGGC+AGG + chr5_1:4611951-4611970 None:intergenic 45.0%
CCTGCCCAAATTAACCTCAA+AGG - chr5_1:4611948-4611967 Msa0712810:CDS 45.0%
GTCACCCAAATTATTGTGGG+AGG - chr5_1:4612073-4612092 Msa0712810:intron 45.0%
TGCATTGAACTCCAGATCAG+CGG + chr5_1:4612104-4612123 None:intergenic 45.0%
GACCCGATAATAACAAGTGG+CGG - chr5_1:4612786-4612805 Msa0712810:intron 45.0%
ACCCGATAATAACAAGTGGC+GGG - chr5_1:4612787-4612806 Msa0712810:intron 45.0%
!! ACCCCGCCACTTGTTATTAT+CGG + chr5_1:4612792-4612811 None:intergenic 45.0%
! GAACATGACTCTAGGTTGGA+AGG - chr5_1:4613104-4613123 Msa0712810:intron 45.0%
ACCTGCATTAGCATTTACGG+TGG - chr5_1:4613384-4613403 Msa0712810:CDS 45.0%
!!! AATTTTAAACTAATAAAAAA+GGG + chr5_1:4610515-4610534 None:intergenic 5.0%
!!! AAATTTTAAACTAATAAAAA+AGG + chr5_1:4610516-4610535 None:intergenic 5.0%
!!! AAAATTCATTTTTTTTTAAT+GGG - chr5_1:4610561-4610580 Msa0712810:intron 5.0%
!!! CCACCATCGGTTTTCTGATC+CGG - chr5_1:4609385-4609404 Msa0712810:CDS 50.0%
TCACCGGATCAGAAAACCGA+TGG + chr5_1:4609391-4609410 None:intergenic 50.0%
! TCGGTTTTCTGATCCGGTGA+AGG - chr5_1:4609391-4609410 Msa0712810:CDS 50.0%
! CGGTTTTCTGATCCGGTGAA+GGG - chr5_1:4609392-4609411 Msa0712810:CDS 50.0%
CCGAAAAACACGGTCTCTGT+GGG + chr5_1:4609477-4609496 None:intergenic 50.0%
! CCCACAGAGACCGTGTTTTT+CGG - chr5_1:4609474-4609493 Msa0712810:CDS 50.0%
! CCACAGAGACCGTGTTTTTC+GGG - chr5_1:4609475-4609494 Msa0712810:CDS 50.0%
! CACAGAGACCGTGTTTTTCG+GGG - chr5_1:4609476-4609495 Msa0712810:CDS 50.0%
CGCAAGCTTGTGACCTAAGT+AGG - chr5_1:4611008-4611027 Msa0712810:CDS 50.0%
CAGTGCTCTTCTTCATCTGG+TGG - chr5_1:4611258-4611277 Msa0712810:intron 50.0%
! CAAACAGGGGCTTTTCTGGA+TGG - chr5_1:4611636-4611655 Msa0712810:intron 50.0%
! AACAGGGGCTTTTCTGGATG+GGG - chr5_1:4611638-4611657 Msa0712810:intron 50.0%
GCAGCTCCAAATGGTCTGTT+TGG - chr5_1:4611660-4611679 Msa0712810:intron 50.0%
TATTAGGCCGTCGCAGTAAG+TGG - chr5_1:4611836-4611855 Msa0712810:intron 50.0%
!! CCCCGCCACTTGTTATTATC+GGG + chr5_1:4612791-4612810 None:intergenic 50.0%
CCCGATAATAACAAGTGGCG+GGG - chr5_1:4612788-4612807 Msa0712810:intron 50.0%
GATCGCGAGAACATGACTCT+AGG - chr5_1:4613096-4613115 Msa0712810:intron 50.0%
GCGAGAACATGACTCTAGGT+TGG - chr5_1:4613100-4613119 Msa0712810:intron 50.0%
GCCACCGTAAATGCTAATGC+AGG + chr5_1:4613388-4613407 None:intergenic 50.0%
CCGGATCAGAAAACCGATGG+TGG + chr5_1:4609388-4609407 None:intergenic 55.0%
CCCGAAAAACACGGTCTCTG+TGG + chr5_1:4609478-4609497 None:intergenic 55.0%
!!! GAGACCGTGTTTTTCGGGGT+CGG - chr5_1:4609480-4609499 Msa0712810:CDS 55.0%
GGGAGGAAATTCCGCTGATC+TGG - chr5_1:4612090-4612109 Msa0712810:intron 55.0%
CATAGCAGGAGACACAGCTG+GGG + chr5_1:4613287-4613306 None:intergenic 55.0%
CCATAGCAGGAGACACAGCT+GGG + chr5_1:4613288-4613307 None:intergenic 55.0%
AGCTGTGTCTCCTGCTATGG+CGG - chr5_1:4613288-4613307 Msa0712810:CDS 55.0%
TCAGGATTCACCGCCATAGC+AGG + chr5_1:4613301-4613320 None:intergenic 55.0%
GACGCCGACCCCGAAAAACA+CGG + chr5_1:4609487-4609506 None:intergenic 60.0%
CTGGATGGGGCAGCTCCAAA+TGG - chr5_1:4611651-4611670 Msa0712810:intron 60.0%
CCCAGCTGTGTCTCCTGCTA+TGG - chr5_1:4613285-4613304 Msa0712810:CDS 60.0%
GCCATAGCAGGAGACACAGC+TGG + chr5_1:4613289-4613308 None:intergenic 60.0%
GGGGCGTGCGATCGGTTAAC+AGG + chr5_1:4613268-4613287 None:intergenic 65.0%
ACACAGCTGGGGCGTGCGAT+CGG + chr5_1:4613276-4613295 None:intergenic 65.0%
CGGGGTCGGCGTCTTGCTGA+CGG - chr5_1:4609494-4609513 Msa0712810:CDS 70.0%
GGTCGGCGTCTTGCTGACGG+TGG - chr5_1:4609497-4609516 Msa0712810:CDS 70.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 4609084 4613718 4609084 ID=Msa0712810;Name=Msa0712810
chr5_1 mRNA 4609084 4613718 4609084 ID=Msa0712810-mRNA-1;Parent=Msa0712810;Name=Msa0712810-mRNA-1;_AED=0.08;_eAED=0.08;_QI=173|1|1|1|1|1|10|277|527
chr5_1 exon 4609084 4609563 4609084 ID=Msa0712810-mRNA-1:exon:1949;Parent=Msa0712810-mRNA-1
chr5_1 exon 4609667 4609738 4609667 ID=Msa0712810-mRNA-1:exon:1948;Parent=Msa0712810-mRNA-1
chr5_1 exon 4609833 4609893 4609833 ID=Msa0712810-mRNA-1:exon:1947;Parent=Msa0712810-mRNA-1
chr5_1 exon 4609993 4610086 4609993 ID=Msa0712810-mRNA-1:exon:1946;Parent=Msa0712810-mRNA-1
chr5_1 exon 4610435 4610514 4610435 ID=Msa0712810-mRNA-1:exon:1945;Parent=Msa0712810-mRNA-1
chr5_1 exon 4610613 4610748 4610613 ID=Msa0712810-mRNA-1:exon:1944;Parent=Msa0712810-mRNA-1
chr5_1 exon 4610952 4611179 4610952 ID=Msa0712810-mRNA-1:exon:1943;Parent=Msa0712810-mRNA-1
chr5_1 exon 4611390 4611631 4611390 ID=Msa0712810-mRNA-1:exon:1942;Parent=Msa0712810-mRNA-1
chr5_1 exon 4611863 4611998 4611863 ID=Msa0712810-mRNA-1:exon:1941;Parent=Msa0712810-mRNA-1
chr5_1 exon 4613214 4613718 4613214 ID=Msa0712810-mRNA-1:exon:1940;Parent=Msa0712810-mRNA-1
chr5_1 five_prime_UTR 4613546 4613718 4613546 ID=Msa0712810-mRNA-1:five_prime_utr;Parent=Msa0712810-mRNA-1
chr5_1 CDS 4613214 4613545 4613214 ID=Msa0712810-mRNA-1:cds;Parent=Msa0712810-mRNA-1
chr5_1 CDS 4611863 4611998 4611863 ID=Msa0712810-mRNA-1:cds;Parent=Msa0712810-mRNA-1
chr5_1 CDS 4611390 4611631 4611390 ID=Msa0712810-mRNA-1:cds;Parent=Msa0712810-mRNA-1
chr5_1 CDS 4610952 4611179 4610952 ID=Msa0712810-mRNA-1:cds;Parent=Msa0712810-mRNA-1
chr5_1 CDS 4610613 4610748 4610613 ID=Msa0712810-mRNA-1:cds;Parent=Msa0712810-mRNA-1
chr5_1 CDS 4610435 4610514 4610435 ID=Msa0712810-mRNA-1:cds;Parent=Msa0712810-mRNA-1
chr5_1 CDS 4609993 4610086 4609993 ID=Msa0712810-mRNA-1:cds;Parent=Msa0712810-mRNA-1
chr5_1 CDS 4609833 4609893 4609833 ID=Msa0712810-mRNA-1:cds;Parent=Msa0712810-mRNA-1
chr5_1 CDS 4609667 4609738 4609667 ID=Msa0712810-mRNA-1:cds;Parent=Msa0712810-mRNA-1
chr5_1 CDS 4609361 4609563 4609361 ID=Msa0712810-mRNA-1:cds;Parent=Msa0712810-mRNA-1
chr5_1 three_prime_UTR 4609084 4609360 4609084 ID=Msa0712810-mRNA-1:three_prime_utr;Parent=Msa0712810-mRNA-1
Gene Sequence

>Msa0712810

ATGGCTTTTCTTTCCATCTCCTACCAAATCCATAATTCTTTATATGTTTCAGTGTTATTAACGTTAATACTATGTTTGATTTCACAATCACATAGGTGCCACGGTTCTAGTTCATTCGGTTTCGATATCCACCATCGGTTTTCTGATCCGGTGAAGGGTATTTTGGGTATTGAGAACGTTCCTGACAAGGGAAGTCGTGAATACTATGTTGCTATGGCCCACAGAGACCGTGTTTTTCGGGGTCGGCGTCTTGCTGACGGTGGTGATGTTGATCAGAAGCTTCTTACGTTTTCTCCAGATAACACCACGTACCAGATTAGTTTGTTTGGATATTTGCATTTTGCCAATGTTTCTGTTGGAACACCAGCTTCATCGTATCTTGTTGCATTAGATACTGGGAGTGACTTGTTCTGGCTCCCTTGCAATTGTACCAAATGTGTCCATGGTATAGAGCTATCAACAGGACAGAAAATTGCTTTTAATATCTATGATAACAAAGAATCATCCACTAGCAAAAATGTTGCCTGCAATAGCAGCTTATGTGAGCAGAAGACACAGTGCTCTTCTTCATCTGGTGGCACTTGTCCATATCAAGTTGAATATCTATCAGAAAATACTTCAACTACTGGTTTCTTGGTAGAGGATGTGTTGCACCTAATTACAGATAATGATGATCAAACTCAACATGCTAACCCACTAATTACTTTTGGTTGTGGGCAAGTTCAAACAGGGGCTTTTCTGGATGGGGCAGCTCCAAATGGTCTGTTTGGACTAGGAATGAGTGATGTATCTGTTCCAAGTATCTTAGCCAAACAAGGGCTAACTTCAAATTCTTTTTCAATGTGTTTTGGTGATGATGGTTTGGGAAGAATCACATTTGGGGATAATAATAGTAGCTTGGACCAAGGAAAAACACCATTCAATATTAGGCCGTCGCATTCAACTTACAACATCACTGTCACCCAAATTATTGTGGGAGGAAATTCCGCTGATCTGGAGTTCAATGCAATATTCGATACTGGTACCTCATTTACATACTTAAACAACCCGGCTTATAAGCAAATTACTCAAAGTTTTGATTCAAAAATTAAACTACAAAGGCATTCATTTTCTGATTCTGATGATCTTCCCTTTGAGTACTGCTATGACTTAAGAACAAACCAGACAATTGAAGTTCCCAATGTTAACTTGACGATGAAAGGTGGAGACAATTATTTTGTCATGGACCCGATAATAACAAGTGGCGGGGGTGTTAACACTGTTCTTTGTTTGGCTGTTTTGAAAAGCAACAATGTGAATATCATTGGACAAAACTTCATGACTGGCTACCGGATAGTTTTTGATCGCGAGAACATGACTCTAGGTTGGAAGGAATCTAACTGTTATGATGATGAACTCTCGAGTTTACCTGTTAACCGATCGCACGCCCCAGCTGTGTCTCCTGCTATGGCGGTGAATCCTGAAATACAATCTACCCCTTCAAATGGTCCTCAAAGACTTCCATCTAGTCACTCATTTAAGAAAGAACCTGCATTAGCATTTACGGTGGCAATTATCCTGCTTTTATCAATTTTTTTAGCATAG
Protein sequence

>Msa0712810

MAFLSISYQIHNSLYVSVLLTLILCLISQSHRCHGSSSFGFDIHHRFSDPVKGILGIENVPDKGSREYYVAMAHRDRVFRGRRLADGGDVDQKLLTFSPDNTTYQISLFGYLHFANVSVGTPASSYLVALDTGSDLFWLPCNCTKCVHGIELSTGQKIAFNIYDNKESSTSKNVACNSSLCEQKTQCSSSSGGTCPYQVEYLSENTSTTGFLVEDVLHLITDNDDQTQHANPLITFGCGQVQTGAFLDGAAPNGLFGLGMSDVSVPSILAKQGLTSNSFSMCFGDDGLGRITFGDNNSSLDQGKTPFNIRPSHSTYNITVTQIIVGGNSADLEFNAIFDTGTSFTYLNNPAYKQITQSFDSKIKLQRHSFSDSDDLPFEYCYDLRTNQTIEVPNVNLTMKGGDNYFVMDPIITSGGGVNTVLCLAVLKSNNVNIIGQNFMTGYRIVFDRENMTLGWKESNCYDDELSSLPVNRSHAPAVSPAMAVNPEIQSTPSNGPQRLPSSHSFKKEPALAFTVAIILLLSIFLA*