AlfalfaGEDB | Msa0714880

M. sativa cultivar zhongmu-4 / Msa0714880

KEGG: -
GO: -
PFAM: PF00010.29

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0714880	XP_003611730.1	92.430	251	13	2	1	249	1	247	3.42e-138	398

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0714880	sp\|Q9FHA7\|HEC1_ARATH	56.771	192	69	4	17	203	4	186	1.97e-49	165

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0714880	G7K6N1	92.430	251	13	2	1	249	1	247	1.64e-138	398

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TFs/TRs:

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Gene ID	Type	Classification
Msa0714880	TF	bHLH

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0343160	Msa0714880	0.808868	2.507078e-50	-8.615850e-47
Msa0714880	Msa0836460	0.897743	1.045671e-76	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0714880	MtrunA17_Chr5g0402161	92.430	251	13	2	1	249	1	247	3.15e-142	398
Msa0714880	MtrunA17_Chr8g0375121	57.143	266	81	10	1	249	1	250	2.50e-65	203
Msa0714880	MtrunA17_Chr3g0134001	55.024	209	75	8	25	214	40	248	4.46e-58	185
Msa0714880	MtrunA17_Chr8g0384141	74.725	91	23	0	112	202	87	177	2.17e-46	155
Msa0714880	MtrunA17_Chr2g0330641	64.486	107	36	2	100	205	251	356	7.34e-32	120
Msa0714880	MtrunA17_Chr8g0341421	63.636	99	32	1	111	205	296	394	3.32e-29	114
Msa0714880	MtrunA17_Chr7g0243041	68.966	58	18	0	145	202	32	89	3.13e-23	92.0
Msa0714880	MtrunA17_Chr5g0435021	55.696	79	33	1	125	201	202	280	1.68e-20	88.6
Msa0714880	MtrunA17_Chr1g0207301	53.731	67	31	0	135	201	284	350	1.47e-17	80.9
Msa0714880	MtrunA17_Chr1g0152651	62.963	54	20	0	148	201	283	336	2.80e-17	80.1

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0714880	AT5G67060.1	56.771	192	69	4	17	203	4	186	2.00e-50	165
Msa0714880	AT3G50330.1	54.587	218	68	7	14	222	4	199	2.68e-50	164
Msa0714880	AT5G67060.2	56.284	183	67	3	25	203	1	174	2.10e-47	157
Msa0714880	AT4G00120.1	66.667	96	29	1	114	206	87	182	3.74e-39	135
Msa0714880	AT3G21330.1	54.472	123	48	1	90	204	212	334	4.14e-38	137
Msa0714880	AT5G09750.1	75.556	90	22	0	112	201	92	181	7.00e-35	124
Msa0714880	AT5G01305.1	69.492	59	18	0	140	198	38	96	7.41e-22	89.4
Msa0714880	AT5G43175.1	50.725	69	34	0	133	201	126	194	7.91e-19	82.8
Msa0714880	AT4G33880.1	59.677	62	25	0	140	201	267	328	1.51e-18	84.0
Msa0714880	AT5G37800.1	56.164	73	25	1	136	201	191	263	3.58e-18	82.4

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Find 58 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGAATGTGAAGATTTCAA+AGG	0.590303	5_1:+7701337	Msa0714880:CDS
AAAGAATTAGTGAAAGAATA+AGG	0.285456	5_1:+7701393	Msa0714880:CDS
AACAGAGTTCAAATCCCATC+AGG	0.513806	5_1:+7701182	Msa0714880:CDS
AATAGACCTTTGAATGTTGT+TGG	0.403761	5_1:+7701536	Msa0714880:CDS
AATGGTGGATTGATCAATGA+AGG	0.629442	5_1:-7701113	None:intergenic
AATTATTCTTCTTTGCTTAG+AGG	0.274535	5_1:+7701590	Msa0714880:CDS
AATTGTTGGTTCTTCCTGAT+GGG	0.499420	5_1:-7701196	None:intergenic
ACAAACAATGATTTCCCTTA+TGG	0.235794	5_1:+7701062	Msa0714880:CDS
ACTAAGCATTTGTTTGGAAG+TGG	0.402015	5_1:-7701642	None:intergenic
ACTCTGTTGGGTTGAAGCGA+CGG	0.575367	5_1:-7701167	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACAAACAATGATTTCCCTTA+TGG	+	chr5_1:7701062-7701081	Msa0714880:CDS	30.0%
ATAGGAGAATGAAGAATTGT+TGG	-	chr5_1:7701213-7701232	None:intergenic	30.0%
ATCTTCACATTCTTTCTCTT+TGG	-	chr5_1:7701332-7701351	None:intergenic	30.0%
AATAGACCTTTGAATGTTGT+TGG	+	chr5_1:7701536-7701555	Msa0714880:CDS	30.0%
ATAGACCTTTGAATGTTGTT+GGG	+	chr5_1:7701537-7701556	Msa0714880:CDS	30.0%
ACAAACAATGATTTCCCTTA+TGG	+	chr5_1:7701062-7701081	Msa0714880:CDS	30.0%
ATAGGAGAATGAAGAATTGT+TGG	-	chr5_1:7701213-7701232	None:intergenic	30.0%
ATCTTCACATTCTTTCTCTT+TGG	-	chr5_1:7701332-7701351	None:intergenic	30.0%
AATAGACCTTTGAATGTTGT+TGG	+	chr5_1:7701536-7701555	Msa0714880:CDS	30.0%
ATAGACCTTTGAATGTTGTT+GGG	+	chr5_1:7701537-7701556	Msa0714880:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_1	gene	7700918	7701667	7700918	ID=Msa0714880;Name=Msa0714880
chr5_1	mRNA	7700918	7701667	7700918	ID=Msa0714880-mRNA-1;Parent=Msa0714880;Name=Msa0714880-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|249
chr5_1	exon	7700918	7701667	7700918	ID=Msa0714880-mRNA-1:exon:3225;Parent=Msa0714880-mRNA-1
chr5_1	CDS	7700918	7701667	7700918	ID=Msa0714880-mRNA-1:cds;Parent=Msa0714880-mRNA-1

Gene Sequence

>Msa0714880

ATGGATGAGGACATACTGAAAACTTTGGGAAATGTTGAAAGTAACATGGACATGATGACAATGATGATGCAAATGGAAAAGTTCCCTGATTCATATGAACCTTTTTATAGCAATACCAATAACAACACTCTCAATCTCAATCTCACAAACAATGATTTCCCTTATGGAAACTCAAATGTAACTTCATCACTCACACCTTCATTGATCAATCCACCATTGTCTTATCCTCTTCAACAACCAATGACACCGCCGTCGCTTCAACCCAACAGAGTTCAAATCCCATCAGGAAGAACCAACAATTCTTCATTCTCCTATCCGGAGAAGAAGAATTCAATGGCAGCAATGAGAGAGATGATATTTCGTATGGCGGTTATGCAACCGATTAACATAGACCCAGAAACCATTAAACCACCAAAGAGAAAGAATGTGAAGATTTCAAAGGATCCACAAAGTGTAGCAGCAAGACATAGAAGAGAAAGAATTAGTGAAAGAATAAGGATATTACAAAGATTAGTACCTGGTGGAACAAAAATGGACACTGCTTCTATGTTAGATGAAGCTATACATTATGTGAAGTTTTTGAAGAAACAAGTTCAAACGTTGGAACAAGTTGGGGCAAATAGACCTTTGAATGTTGTTGGGTTTCAAGGAGTGGTTTCTAATGCTAATTTTAATTATTCTTCTTTGCTTAGAGGTTGTTCATCACCTTGCCAAATGGTGGGTCCCACTTCCAAACAAATGCTTAGTTGA

Protein sequence

>Msa0714880

MDEDILKTLGNVESNMDMMTMMMQMEKFPDSYEPFYSNTNNNTLNLNLTNNDFPYGNSNVTSSLTPSLINPPLSYPLQQPMTPPSLQPNRVQIPSGRTNNSSFSYPEKKNSMAAMREMIFRMAVMQPINIDPETIKPPKRKNVKISKDPQSVAARHRRERISERIRILQRLVPGGTKMDTASMLDEAIHYVKFLKKQVQTLEQVGANRPLNVVGFQGVVSNANFNYSSLLRGCSSPCQMVGPTSKQMLS*