AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0716820

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0716820 MtrunA17_Chr5g0404131 90.377 239 12 5 1 232 71 305 3.75e-148 415
Msa0716820 MtrunA17_Chr8g0373231 55.556 243 82 8 1 232 1 228 3.27e-59 186
Msa0716820 MtrunA17_Chr1g0151461 38.889 90 52 2 89 177 155 242 2.45e-14 70.9
Msa0716820 MtrunA17_Chr7g0271861 56.250 48 19 1 119 166 18 63 2.75e-13 64.7
Msa0716820 MtrunA17_Chr2g0326771 40.000 60 36 0 96 155 189 248 8.24e-13 66.6
Msa0716820 MtrunA17_Chr4g0068031 67.568 37 12 0 123 159 30 66 1.06e-12 63.5
Msa0716820 MtrunA17_Chr4g0071301 69.048 42 12 1 111 152 181 221 1.40e-12 65.9
Msa0716820 MtrunA17_Chr4g0028961 32.143 84 52 1 72 155 186 264 1.08e-11 63.2
Msa0716820 MtrunA17_Chr1g0213161 62.857 35 13 0 121 155 150 184 1.84e-11 62.0
Msa0716820 MtrunA17_Chr5g0404981 38.571 70 41 1 88 155 263 332 5.76e-11 61.6
Msa0716820 MtrunA17_Chr1g0199671 62.162 37 14 0 119 155 238 274 6.40e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0716820 AT3G50870.1 41.281 281 104 10 5 232 9 281 1.66e-48 161
Msa0716820 AT4G36620.1 43.891 221 83 10 27 232 3 197 7.21e-39 134
Msa0716820 AT2G18380.1 41.304 230 93 8 19 230 2 207 1.69e-36 128
Msa0716820 AT5G56860.1 49.462 93 33 3 64 156 185 263 5.78e-17 79.3
Msa0716820 AT5G49300.1 53.731 67 27 2 98 162 11 75 4.49e-15 70.1
Msa0716820 AT4G26150.1 63.830 47 16 1 107 153 184 229 1.16e-13 69.7
Msa0716820 AT1G08010.2 45.070 71 33 2 105 170 203 272 1.38e-12 66.2
Msa0716820 AT1G08010.1 45.070 71 33 2 105 170 203 272 1.38e-12 66.2
Msa0716820 AT1G08010.3 45.070 71 33 2 105 170 203 272 1.38e-12 66.2
Msa0716820 AT3G54810.1 37.234 94 54 1 106 194 212 305 4.33e-12 65.1
Msa0716820 AT3G54810.2 37.234 94 54 1 106 194 212 305 4.33e-12 65.1
Msa0716820 AT5G26930.1 67.568 37 12 0 123 159 26 62 4.39e-12 61.6
Msa0716820 AT3G06740.1 71.429 35 10 0 125 159 43 77 7.18e-12 61.6
Msa0716820 AT3G20750.1 70.588 34 10 0 127 160 163 196 1.66e-11 62.0
Msa0716820 AT2G45050.1 36.585 82 45 2 74 155 137 211 7.10e-11 60.8
Msa0716820 AT4G34680.1 50.980 51 23 1 107 155 162 212 8.06e-11 60.8
Msa0716820 AT4G34680.2 50.980 51 23 1 107 155 162 212 8.06e-11 60.8

Find 73 sgRNAs with CRISPR-Local

Find 160 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCAATCATGATTCTGAAAA+TGG 0.202815 5_1:-10384789 Msa0716820:CDS
GGAGTGAAACAATAGGTTTC+AGG 0.229794 5_1:+10385412 None:intergenic
GTTGGAGTTGTTGTTATTAT+TGG 0.243633 5_1:+10385198 None:intergenic
CTTCATTTCATTAATTTCAT+TGG 0.284470 5_1:-10385786 Msa0716820:five_prime_UTR
GAAGAAGAAGATGAAGAATT+AGG 0.286532 5_1:+10385391 None:intergenic
ATGGCATTCCATTTCTCTCT+TGG 0.336081 5_1:-10384770 Msa0716820:CDS
GAGTGAATGGTAATAATAAA+AGG 0.354102 5_1:+10385600 None:intergenic
GAGAGAAACACTGCATCTTC+TGG 0.356763 5_1:+10384847 None:intergenic
TTCATTTCATTAATTTCATT+GGG 0.375952 5_1:-10385785 Msa0716820:five_prime_UTR
AGTGTTTGAAGATAATAAAA+TGG 0.376545 5_1:+10385654 None:intergenic
AACGCCATCTATCGGCATAA+TGG 0.385803 5_1:-10384931 Msa0716820:CDS
CAGGGAGTGAGTGTGATTGT+AGG 0.390962 5_1:+10385816 None:intergenic
AATGAAAATGAATGATTTGA+AGG 0.402129 5_1:+10385559 None:intergenic
CGGAACTCGTTAGTCATTGC+TGG 0.406108 5_1:+10384825 None:intergenic
TGTTCATGTGGTATGTTTCA+TGG 0.414708 5_1:-10385481 Msa0716820:CDS
CCACCTCCACACCGCTTTGG+AGG 0.424846 5_1:-10385135 Msa0716820:CDS
TAGGTTTCAGGTTCATCATA+AGG 0.424993 5_1:+10385424 None:intergenic
AATGAAATGAAGGGTAAGTC+AGG 0.429512 5_1:+10385797 None:intergenic
ATGCCGATAGATGGCGTTGC+CGG 0.430815 5_1:+10384936 None:intergenic
TTAGAGTAGAGAAGAGTGAA+TGG 0.442320 5_1:+10385587 None:intergenic
TAGAGTCTCCAAGAGAGAAA+TGG 0.442835 5_1:+10384762 None:intergenic
CAATGAAATTAATGAAATGA+AGG 0.448951 5_1:+10385787 None:intergenic
ATAGTAAAGGAGATAAATAG+AGG 0.453860 5_1:+10385756 None:intergenic
AGAATTAGGAGTGAAACAAT+AGG 0.458450 5_1:+10385405 None:intergenic
AGGGAGTGAGTGTGATTGTA+GGG 0.460006 5_1:+10385817 None:intergenic
AGAAGAGCAACTGCTGCAGC+CGG 0.462808 5_1:-10384963 Msa0716820:CDS
ACACCACCTCCACACCGCTT+TGG 0.463073 5_1:-10385138 Msa0716820:CDS
GATTCCATTATGCCGATAGA+TGG 0.464364 5_1:+10384927 None:intergenic
CCTCCAAAGCGGTGTGGAGG+TGG 0.470303 5_1:+10385135 None:intergenic
TATCTCTGCAGAATTACTAC+TGG 0.478719 5_1:+10385874 None:intergenic
TGAGTACTGTTGTACTGTTT+AGG 0.483115 5_1:+10385250 None:intergenic
CACTGCATCTTCTGGTTCTG+TGG 0.485352 5_1:+10384855 None:intergenic
AGCAAGAAGAGAATCATTGT+TGG 0.486549 5_1:+10385180 None:intergenic
GATTGGTTTCATCAATGAAA+CGG 0.487226 5_1:+10384805 None:intergenic
AACTGCTGCAGCCGGCACAC+CGG 0.491074 5_1:-10384955 Msa0716820:CDS
AGATGGCGTTGCCGGTGTGC+CGG 0.497703 5_1:+10384944 None:intergenic
ATGAAAATGAATGATTTGAA+GGG 0.497940 5_1:+10385560 None:intergenic
ACGTTGTCGTGAATTGCAAA+TGG 0.506728 5_1:+10385704 None:intergenic
ATCTCAAGGGCAACACGTGA+TGG 0.509206 5_1:-10385509 Msa0716820:CDS
TCCACACCGCTTTGGAGGAA+CGG 0.515774 5_1:-10385130 Msa0716820:CDS
TTCACGACAACGTGGACTGC+AGG 0.517568 5_1:-10385694 Msa0716820:five_prime_UTR
GTTGATTGTACTCTTTCACT+TGG 0.527724 5_1:-10385367 Msa0716820:CDS
ATGATGCATCGTTCATCTCA+AGG 0.533388 5_1:-10385523 Msa0716820:CDS
TAAAGGAGATAAATAGAGGG+AGG 0.538887 5_1:+10385760 None:intergenic
CAGAACGACGTTCATGATGT+CGG 0.540322 5_1:+10385302 None:intergenic
ATACATACTTACTTACATGA+AGG 0.540444 5_1:-10385907 None:intergenic
ATACAAAATATATATAGTAA+AGG 0.543734 5_1:+10385743 None:intergenic
ACACCGGCAACGCCATCTAT+CGG 0.547655 5_1:-10384939 Msa0716820:CDS
TGAAAATGAATGATTTGAAG+GGG 0.547996 5_1:+10385561 None:intergenic
TAGTAAAGGAGATAAATAGA+GGG 0.561536 5_1:+10385757 None:intergenic
TCTCAAGGGCAACACGTGAT+GGG 0.577571 5_1:-10385508 Msa0716820:CDS
AAAGGAGATAAATAGAGGGA+GGG 0.578878 5_1:+10385761 None:intergenic
CAGTCACTATGCAATGCATG+TGG 0.582954 5_1:-10385008 Msa0716820:CDS
TACATACTTACTTACATGAA+GGG 0.583328 5_1:-10385906 None:intergenic
ACCACCATCACAACAACTCA+TGG 0.584082 5_1:-10384887 Msa0716820:CDS
CAGTACTCAGTCTCAAACAA+AGG 0.584934 5_1:-10385236 Msa0716820:CDS
TCTTCAGTGAAACGAGTGGA+TGG 0.587900 5_1:+10385340 None:intergenic
ATGAGTTGTTGTGATGGTGG+TGG 0.588375 5_1:+10384889 None:intergenic
AATGAAATTAATGAAATGAA+GGG 0.589066 5_1:+10385788 None:intergenic
TGATGCATCGTTCATCTCAA+GGG 0.590177 5_1:-10385522 Msa0716820:CDS
GTTCCTCCAAAGCGGTGTGG+AGG 0.595180 5_1:+10385132 None:intergenic
ATGAAATGAAGGGTAAGTCA+GGG 0.605058 5_1:+10385798 None:intergenic
GAACGGTCCACGAGGCCCAA+AGG 0.609147 5_1:-10385113 Msa0716820:intron
ACCGTTCCTCCAAAGCGGTG+TGG 0.617219 5_1:+10385129 None:intergenic
TTCATCTTCAGTGAAACGAG+TGG 0.627984 5_1:+10385336 None:intergenic
ATGTGGGATCAGATTCAAGA+AGG 0.634149 5_1:-10384991 Msa0716820:CDS
ACCATGAGTTGTTGTGATGG+TGG 0.656581 5_1:+10384886 None:intergenic
AGTCACTATGCAATGCATGT+GGG 0.668102 5_1:-10385007 Msa0716820:CDS
CGTACCATGAGTTGTTGTGA+TGG 0.677789 5_1:+10384883 None:intergenic
CGTGGACCGTTCCTCCAAAG+CGG 0.688334 5_1:+10385124 None:intergenic
ATTTGCAATTCACGACAACG+TGG 0.691520 5_1:-10385702 Msa0716820:five_prime_UTR
CTTTGGAGGAACGGTCCACG+AGG 0.702547 5_1:-10385121 Msa0716820:CDS
GTGATGGGACACTGTTCATG+TGG 0.723745 5_1:-10385493 Msa0716820:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATACAAAATATATATAGTAA+AGG + chr5_1:10384922-10384941 None:intergenic 10.0%
!! ATACAAAATATATATAGTAA+AGG + chr5_1:10384922-10384941 None:intergenic 10.0%
!! AATGAAATTAATGAAATGAA+GGG + chr5_1:10384877-10384896 None:intergenic 15.0%
!! TTCATTTCATTAATTTCATT+GGG - chr5_1:10384877-10384896 Msa0716820:CDS 15.0%
!! TAAAATGTTATATTTCATGT+TGG + chr5_1:10385619-10385638 None:intergenic 15.0%
!!! ATATAACATTTTATGTTGAA+TGG - chr5_1:10385626-10385645 Msa0716820:five_prime_UTR 15.0%
!! AATGAAATTAATGAAATGAA+GGG + chr5_1:10384877-10384896 None:intergenic 15.0%
!! TTCATTTCATTAATTTCATT+GGG - chr5_1:10384877-10384896 Msa0716820:CDS 15.0%
!! TAAAATGTTATATTTCATGT+TGG + chr5_1:10385619-10385638 None:intergenic 15.0%
!!! ATATAACATTTTATGTTGAA+TGG - chr5_1:10385626-10385645 Msa0716820:five_prime_UTR 15.0%
!! CAATGAAATTAATGAAATGA+AGG + chr5_1:10384878-10384897 None:intergenic 20.0%
!! CTTCATTTCATTAATTTCAT+TGG - chr5_1:10384876-10384895 Msa0716820:CDS 20.0%
!! AGTGTTTGAAGATAATAAAA+TGG + chr5_1:10385011-10385030 None:intergenic 20.0%
!! ATGAAAATGAATGATTTGAA+GGG + chr5_1:10385105-10385124 None:intergenic 20.0%
!! AATGAAAATGAATGATTTGA+AGG + chr5_1:10385106-10385125 None:intergenic 20.0%
!! TTATATTTCATGTTGGTATT+TGG + chr5_1:10385612-10385631 None:intergenic 20.0%
!! CAATGAAATTAATGAAATGA+AGG + chr5_1:10384878-10384897 None:intergenic 20.0%
!! CTTCATTTCATTAATTTCAT+TGG - chr5_1:10384876-10384895 Msa0716820:CDS 20.0%
!! AGTGTTTGAAGATAATAAAA+TGG + chr5_1:10385011-10385030 None:intergenic 20.0%
!! ATGAAAATGAATGATTTGAA+GGG + chr5_1:10385105-10385124 None:intergenic 20.0%
!! AATGAAAATGAATGATTTGA+AGG + chr5_1:10385106-10385125 None:intergenic 20.0%
!! TTATATTTCATGTTGGTATT+TGG + chr5_1:10385612-10385631 None:intergenic 20.0%
! TAGTAAAGGAGATAAATAGA+GGG + chr5_1:10384908-10384927 None:intergenic 25.0%
! ATAGTAAAGGAGATAAATAG+AGG + chr5_1:10384909-10384928 None:intergenic 25.0%
! GAGTGAATGGTAATAATAAA+AGG + chr5_1:10385065-10385084 None:intergenic 25.0%
! TGAAAATGAATGATTTGAAG+GGG + chr5_1:10385104-10385123 None:intergenic 25.0%
! TTCTGTCAAAAATTTCTGTT+GGG - chr5_1:10385378-10385397 Msa0716820:CDS 25.0%
!!! TGTAGAGTATTTTTACCTTT+GGG + chr5_1:10385567-10385586 None:intergenic 25.0%
!!! ATGTAGAGTATTTTTACCTT+TGG + chr5_1:10385568-10385587 None:intergenic 25.0%
! TAGTAAAGGAGATAAATAGA+GGG + chr5_1:10384908-10384927 None:intergenic 25.0%
! ATAGTAAAGGAGATAAATAG+AGG + chr5_1:10384909-10384928 None:intergenic 25.0%
! GAGTGAATGGTAATAATAAA+AGG + chr5_1:10385065-10385084 None:intergenic 25.0%
! TGAAAATGAATGATTTGAAG+GGG + chr5_1:10385104-10385123 None:intergenic 25.0%
! TTCTGTCAAAAATTTCTGTT+GGG - chr5_1:10385378-10385397 Msa0716820:CDS 25.0%
!!! TGTAGAGTATTTTTACCTTT+GGG + chr5_1:10385567-10385586 None:intergenic 25.0%
!!! ATGTAGAGTATTTTTACCTT+TGG + chr5_1:10385568-10385587 None:intergenic 25.0%
AGAATTAGGAGTGAAACAAT+AGG + chr5_1:10385260-10385279 None:intergenic 30.0%
GAAGAAGAAGATGAAGAATT+AGG + chr5_1:10385274-10385293 None:intergenic 30.0%
GTTCTGTCAAAAATTTCTGT+TGG - chr5_1:10385377-10385396 Msa0716820:CDS 30.0%
! GTTGGAGTTGTTGTTATTAT+TGG + chr5_1:10385467-10385486 None:intergenic 30.0%
!! GATTGGTTTCATCAATGAAA+CGG + chr5_1:10385860-10385879 None:intergenic 30.0%
! ACCAATCATGATTCTGAAAA+TGG - chr5_1:10385873-10385892 Msa0716820:five_prime_UTR 30.0%
AGAATTAGGAGTGAAACAAT+AGG + chr5_1:10385260-10385279 None:intergenic 30.0%
GAAGAAGAAGATGAAGAATT+AGG + chr5_1:10385274-10385293 None:intergenic 30.0%
GTTCTGTCAAAAATTTCTGT+TGG - chr5_1:10385377-10385396 Msa0716820:CDS 30.0%
! GTTGGAGTTGTTGTTATTAT+TGG + chr5_1:10385467-10385486 None:intergenic 30.0%
!! GATTGGTTTCATCAATGAAA+CGG + chr5_1:10385860-10385879 None:intergenic 30.0%
! ACCAATCATGATTCTGAAAA+TGG - chr5_1:10385873-10385892 Msa0716820:five_prime_UTR 30.0%
TATCTCTGCAGAATTACTAC+TGG + chr5_1:10384791-10384810 None:intergenic 35.0%
ATGAAATGAAGGGTAAGTCA+GGG + chr5_1:10384867-10384886 None:intergenic 35.0%
AATGAAATGAAGGGTAAGTC+AGG + chr5_1:10384868-10384887 None:intergenic 35.0%
AAAGGAGATAAATAGAGGGA+GGG + chr5_1:10384904-10384923 None:intergenic 35.0%
TAAAGGAGATAAATAGAGGG+AGG + chr5_1:10384905-10384924 None:intergenic 35.0%
TTAGAGTAGAGAAGAGTGAA+TGG + chr5_1:10385078-10385097 None:intergenic 35.0%
TGTTCATGTGGTATGTTTCA+TGG - chr5_1:10385181-10385200 Msa0716820:CDS 35.0%
!! TAGGTTTCAGGTTCATCATA+AGG + chr5_1:10385241-10385260 None:intergenic 35.0%
GTTGATTGTACTCTTTCACT+TGG - chr5_1:10385295-10385314 Msa0716820:CDS 35.0%
! TGAGTACTGTTGTACTGTTT+AGG + chr5_1:10385415-10385434 None:intergenic 35.0%
AGCAAGAAGAGAATCATTGT+TGG + chr5_1:10385485-10385504 None:intergenic 35.0%
!! GCCATTTTCAGAATCATGAT+TGG + chr5_1:10385877-10385896 None:intergenic 35.0%
TATCTCTGCAGAATTACTAC+TGG + chr5_1:10384791-10384810 None:intergenic 35.0%
ATGAAATGAAGGGTAAGTCA+GGG + chr5_1:10384867-10384886 None:intergenic 35.0%
AATGAAATGAAGGGTAAGTC+AGG + chr5_1:10384868-10384887 None:intergenic 35.0%
AAAGGAGATAAATAGAGGGA+GGG + chr5_1:10384904-10384923 None:intergenic 35.0%
TAAAGGAGATAAATAGAGGG+AGG + chr5_1:10384905-10384924 None:intergenic 35.0%
TTAGAGTAGAGAAGAGTGAA+TGG + chr5_1:10385078-10385097 None:intergenic 35.0%
TGTTCATGTGGTATGTTTCA+TGG - chr5_1:10385181-10385200 Msa0716820:CDS 35.0%
!! TAGGTTTCAGGTTCATCATA+AGG + chr5_1:10385241-10385260 None:intergenic 35.0%
GTTGATTGTACTCTTTCACT+TGG - chr5_1:10385295-10385314 Msa0716820:CDS 35.0%
! TGAGTACTGTTGTACTGTTT+AGG + chr5_1:10385415-10385434 None:intergenic 35.0%
AGCAAGAAGAGAATCATTGT+TGG + chr5_1:10385485-10385504 None:intergenic 35.0%
!! GCCATTTTCAGAATCATGAT+TGG + chr5_1:10385877-10385896 None:intergenic 35.0%
ACGTTGTCGTGAATTGCAAA+TGG + chr5_1:10384961-10384980 None:intergenic 40.0%
ATTTGCAATTCACGACAACG+TGG - chr5_1:10384960-10384979 Msa0716820:CDS 40.0%
ATGATGCATCGTTCATCTCA+AGG - chr5_1:10385139-10385158 Msa0716820:CDS 40.0%
TGATGCATCGTTCATCTCAA+GGG - chr5_1:10385140-10385159 Msa0716820:CDS 40.0%
GGAGTGAAACAATAGGTTTC+AGG + chr5_1:10385253-10385272 None:intergenic 40.0%
TTCATCTTCAGTGAAACGAG+TGG + chr5_1:10385329-10385348 None:intergenic 40.0%
CAGTACTCAGTCTCAAACAA+AGG - chr5_1:10385426-10385445 Msa0716820:CDS 40.0%
AGTCACTATGCAATGCATGT+GGG - chr5_1:10385655-10385674 Msa0716820:five_prime_UTR 40.0%
ATGTGGGATCAGATTCAAGA+AGG - chr5_1:10385671-10385690 Msa0716820:five_prime_UTR 40.0%
GATTCCATTATGCCGATAGA+TGG + chr5_1:10385738-10385757 None:intergenic 40.0%
ATGGCATTCCATTTCTCTCT+TGG - chr5_1:10385892-10385911 Msa0716820:five_prime_UTR 40.0%
TAGAGTCTCCAAGAGAGAAA+TGG + chr5_1:10385903-10385922 None:intergenic 40.0%
ACGTTGTCGTGAATTGCAAA+TGG + chr5_1:10384961-10384980 None:intergenic 40.0%
ATTTGCAATTCACGACAACG+TGG - chr5_1:10384960-10384979 Msa0716820:CDS 40.0%
ATGATGCATCGTTCATCTCA+AGG - chr5_1:10385139-10385158 Msa0716820:CDS 40.0%
TGATGCATCGTTCATCTCAA+GGG - chr5_1:10385140-10385159 Msa0716820:CDS 40.0%
GGAGTGAAACAATAGGTTTC+AGG + chr5_1:10385253-10385272 None:intergenic 40.0%
TTCATCTTCAGTGAAACGAG+TGG + chr5_1:10385329-10385348 None:intergenic 40.0%
CAGTACTCAGTCTCAAACAA+AGG - chr5_1:10385426-10385445 Msa0716820:CDS 40.0%
AGTCACTATGCAATGCATGT+GGG - chr5_1:10385655-10385674 Msa0716820:five_prime_UTR 40.0%
ATGTGGGATCAGATTCAAGA+AGG - chr5_1:10385671-10385690 Msa0716820:five_prime_UTR 40.0%
GATTCCATTATGCCGATAGA+TGG + chr5_1:10385738-10385757 None:intergenic 40.0%
ATGGCATTCCATTTCTCTCT+TGG - chr5_1:10385892-10385911 Msa0716820:five_prime_UTR 40.0%
TAGAGTCTCCAAGAGAGAAA+TGG + chr5_1:10385903-10385922 None:intergenic 40.0%
AGGGAGTGAGTGTGATTGTA+GGG + chr5_1:10384848-10384867 None:intergenic 45.0%
TCTTCAGTGAAACGAGTGGA+TGG + chr5_1:10385325-10385344 None:intergenic 45.0%
CAGAACGACGTTCATGATGT+CGG + chr5_1:10385363-10385382 None:intergenic 45.0%
! ATTTTTACCTTTGGGCCTCG+TGG + chr5_1:10385559-10385578 None:intergenic 45.0%
CAGTCACTATGCAATGCATG+TGG - chr5_1:10385654-10385673 Msa0716820:five_prime_UTR 45.0%
AACGCCATCTATCGGCATAA+TGG - chr5_1:10385731-10385750 Msa0716820:five_prime_UTR 45.0%
!! ATGAGTTGTTGTGATGGTGG+TGG + chr5_1:10385776-10385795 None:intergenic 45.0%
ACCACCATCACAACAACTCA+TGG - chr5_1:10385775-10385794 Msa0716820:five_prime_UTR 45.0%
ACCATGAGTTGTTGTGATGG+TGG + chr5_1:10385779-10385798 None:intergenic 45.0%
CGTACCATGAGTTGTTGTGA+TGG + chr5_1:10385782-10385801 None:intergenic 45.0%
GAGAGAAACACTGCATCTTC+TGG + chr5_1:10385818-10385837 None:intergenic 45.0%
AGGGAGTGAGTGTGATTGTA+GGG + chr5_1:10384848-10384867 None:intergenic 45.0%
TCTTCAGTGAAACGAGTGGA+TGG + chr5_1:10385325-10385344 None:intergenic 45.0%
CAGAACGACGTTCATGATGT+CGG + chr5_1:10385363-10385382 None:intergenic 45.0%
! ATTTTTACCTTTGGGCCTCG+TGG + chr5_1:10385559-10385578 None:intergenic 45.0%
CAGTCACTATGCAATGCATG+TGG - chr5_1:10385654-10385673 Msa0716820:five_prime_UTR 45.0%
AACGCCATCTATCGGCATAA+TGG - chr5_1:10385731-10385750 Msa0716820:five_prime_UTR 45.0%
!! ATGAGTTGTTGTGATGGTGG+TGG + chr5_1:10385776-10385795 None:intergenic 45.0%
ACCACCATCACAACAACTCA+TGG - chr5_1:10385775-10385794 Msa0716820:five_prime_UTR 45.0%
ACCATGAGTTGTTGTGATGG+TGG + chr5_1:10385779-10385798 None:intergenic 45.0%
CGTACCATGAGTTGTTGTGA+TGG + chr5_1:10385782-10385801 None:intergenic 45.0%
GAGAGAAACACTGCATCTTC+TGG + chr5_1:10385818-10385837 None:intergenic 45.0%
CAGGGAGTGAGTGTGATTGT+AGG + chr5_1:10384849-10384868 None:intergenic 50.0%
ATCTCAAGGGCAACACGTGA+TGG - chr5_1:10385153-10385172 Msa0716820:CDS 50.0%
TCTCAAGGGCAACACGTGAT+GGG - chr5_1:10385154-10385173 Msa0716820:CDS 50.0%
! GTGATGGGACACTGTTCATG+TGG - chr5_1:10385169-10385188 Msa0716820:CDS 50.0%
!! CACTGCATCTTCTGGTTCTG+TGG + chr5_1:10385810-10385829 None:intergenic 50.0%
CGGAACTCGTTAGTCATTGC+TGG + chr5_1:10385840-10385859 None:intergenic 50.0%
CAGGGAGTGAGTGTGATTGT+AGG + chr5_1:10384849-10384868 None:intergenic 50.0%
ATCTCAAGGGCAACACGTGA+TGG - chr5_1:10385153-10385172 Msa0716820:CDS 50.0%
TCTCAAGGGCAACACGTGAT+GGG - chr5_1:10385154-10385173 Msa0716820:CDS 50.0%
! GTGATGGGACACTGTTCATG+TGG - chr5_1:10385169-10385188 Msa0716820:CDS 50.0%
!! CACTGCATCTTCTGGTTCTG+TGG + chr5_1:10385810-10385829 None:intergenic 50.0%
CGGAACTCGTTAGTCATTGC+TGG + chr5_1:10385840-10385859 None:intergenic 50.0%
! TTCACGACAACGTGGACTGC+AGG - chr5_1:10384968-10384987 Msa0716820:CDS 55.0%
!! TCCACACCGCTTTGGAGGAA+CGG - chr5_1:10385532-10385551 Msa0716820:exon 55.0%
AGAAGAGCAACTGCTGCAGC+CGG - chr5_1:10385699-10385718 Msa0716820:five_prime_UTR 55.0%
ACACCGGCAACGCCATCTAT+CGG - chr5_1:10385723-10385742 Msa0716820:five_prime_UTR 55.0%
ATGCCGATAGATGGCGTTGC+CGG + chr5_1:10385729-10385748 None:intergenic 55.0%
! TTCACGACAACGTGGACTGC+AGG - chr5_1:10384968-10384987 Msa0716820:CDS 55.0%
!! TCCACACCGCTTTGGAGGAA+CGG - chr5_1:10385532-10385551 Msa0716820:exon 55.0%
AGAAGAGCAACTGCTGCAGC+CGG - chr5_1:10385699-10385718 Msa0716820:five_prime_UTR 55.0%
ACACCGGCAACGCCATCTAT+CGG - chr5_1:10385723-10385742 Msa0716820:five_prime_UTR 55.0%
ATGCCGATAGATGGCGTTGC+CGG + chr5_1:10385729-10385748 None:intergenic 55.0%
ACACCACCTCCACACCGCTT+TGG - chr5_1:10385524-10385543 Msa0716820:CDS 60.0%
GTTCCTCCAAAGCGGTGTGG+AGG + chr5_1:10385533-10385552 None:intergenic 60.0%
ACCGTTCCTCCAAAGCGGTG+TGG + chr5_1:10385536-10385555 None:intergenic 60.0%
CGTGGACCGTTCCTCCAAAG+CGG + chr5_1:10385541-10385560 None:intergenic 60.0%
! CTTTGGAGGAACGGTCCACG+AGG - chr5_1:10385541-10385560 Msa0716820:exon 60.0%
ACACCACCTCCACACCGCTT+TGG - chr5_1:10385524-10385543 Msa0716820:CDS 60.0%
GTTCCTCCAAAGCGGTGTGG+AGG + chr5_1:10385533-10385552 None:intergenic 60.0%
ACCGTTCCTCCAAAGCGGTG+TGG + chr5_1:10385536-10385555 None:intergenic 60.0%
CGTGGACCGTTCCTCCAAAG+CGG + chr5_1:10385541-10385560 None:intergenic 60.0%
! CTTTGGAGGAACGGTCCACG+AGG - chr5_1:10385541-10385560 Msa0716820:exon 60.0%
CCTCCAAAGCGGTGTGGAGG+TGG + chr5_1:10385530-10385549 None:intergenic 65.0%
CCACCTCCACACCGCTTTGG+AGG - chr5_1:10385527-10385546 Msa0716820:exon 65.0%
GAACGGTCCACGAGGCCCAA+AGG - chr5_1:10385549-10385568 Msa0716820:five_prime_UTR 65.0%
AACTGCTGCAGCCGGCACAC+CGG - chr5_1:10385707-10385726 Msa0716820:five_prime_UTR 65.0%
AGATGGCGTTGCCGGTGTGC+CGG + chr5_1:10385721-10385740 None:intergenic 65.0%
CCTCCAAAGCGGTGTGGAGG+TGG + chr5_1:10385530-10385549 None:intergenic 65.0%
CCACCTCCACACCGCTTTGG+AGG - chr5_1:10385527-10385546 Msa0716820:exon 65.0%
GAACGGTCCACGAGGCCCAA+AGG - chr5_1:10385549-10385568 Msa0716820:five_prime_UTR 65.0%
AACTGCTGCAGCCGGCACAC+CGG - chr5_1:10385707-10385726 Msa0716820:five_prime_UTR 65.0%
AGATGGCGTTGCCGGTGTGC+CGG + chr5_1:10385721-10385740 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 10384761 10385923 10384761 ID=Msa0716820;Name=Msa0716820
chr5_1 mRNA 10384761 10385923 10384761 ID=Msa0716820-mRNA-1;Parent=Msa0716820;Name=Msa0716820-mRNA-1;_AED=0.00;_eAED=0.00;_QI=378|1|0.5|1|1|1|2|0|232
chr5_1 exon 10384761 10385027 10384761 ID=Msa0716820-mRNA-1:exon:4147;Parent=Msa0716820-mRNA-1
chr5_1 exon 10385114 10385923 10385114 ID=Msa0716820-mRNA-1:exon:4146;Parent=Msa0716820-mRNA-1
chr5_1 five_prime_UTR 10385546 10385923 10385546 ID=Msa0716820-mRNA-1:five_prime_utr;Parent=Msa0716820-mRNA-1
chr5_1 CDS 10385114 10385545 10385114 ID=Msa0716820-mRNA-1:cds;Parent=Msa0716820-mRNA-1
chr5_1 CDS 10384761 10385027 10384761 ID=Msa0716820-mRNA-1:cds;Parent=Msa0716820-mRNA-1
Gene Sequence

>Msa0716820

ATGATGCATCGTTCATCTCAAGGGCAACACGTGATGGGACACTGTTCATGTGGTATGTTTCATGGACAAAACAACTCATTCTCAATGTTATTCTCTACACCTTATGATGAACCTGAAACCTATTGTTTCACTCCTAATTCTTCATCTTCTTCTTCTGTTGATTGTACTCTTTCACTTGGAACACCATCCACTCGTTTCACTGAAGATGAAGAGAAAAGAAGCCGACATCATGAACGTCGTTCTGTCAAAAATTTCTGTTGGGATTTGCTAACACCTAAACAGTACAACAGTACTCAGTCTCAAACAAAGGCAACACGTGCAAATACCAATAATAACAACAACTCCAACAATGATTCTCTTCTTGCTCGTAGATGTGCAAACTGTGACACCACCTCCACACCGCTTTGGAGGAACGGTCCACGAGGCCCAAAGTCACTATGCAATGCATGTGGGATCAGATTCAAGAAGGAAGAGAGAAGAGCAACTGCTGCAGCCGGCACACCGGCAACGCCATCTATCGGCATAATGGAATCAGCTTCGATGTATAACCACCACCATCACAACAACTCATGGTACGTACAACCACAGAACCAGAAGATGCAGTGTTTCTCTCCAGCAATGACTAACGAGTTCCGTTTCATTGATGAAACCAATCATGATTCTGAAAATGGCATTCCATTTCTCTCTTGGAGACTCTAA
Protein sequence

>Msa0716820

MMHRSSQGQHVMGHCSCGMFHGQNNSFSMLFSTPYDEPETYCFTPNSSSSSSVDCTLSLGTPSTRFTEDEEKRSRHHERRSVKNFCWDLLTPKQYNSTQSQTKATRANTNNNNNSNNDSLLARRCANCDTTSTPLWRNGPRGPKSLCNACGIRFKKEERRATAAAGTPATPSIGIMESASMYNHHHHNNSWYVQPQNQKMQCFSPAMTNEFRFIDETNHDSENGIPFLSWRL*