AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0717140

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0717140 MtrunA17_Chr1g0183031 94.366 71 4 0 33 103 98 168 2.63e-41 139
Msa0717140 MtrunA17_Chr7g0262551 60.563 71 28 0 33 103 94 164 1.44e-25 97.8
Msa0717140 MtrunA17_Chr1g0183021 63.380 71 26 0 33 103 196 266 5.55e-20 83.2
Msa0717140 MtrunA17_Chr7g0262571 52.113 71 34 0 33 103 149 219 1.35e-19 82.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0717140 AT4G28706.3 63.380 71 26 0 33 103 133 203 3.14e-25 97.8
Msa0717140 AT4G28706.1 63.380 71 26 0 33 103 131 201 3.22e-25 97.8
Msa0717140 AT4G28706.2 63.380 71 26 0 33 103 133 203 3.27e-25 97.8
Msa0717140 AT4G28706.4 63.380 71 26 0 33 103 133 203 4.17e-25 97.8
Msa0717140 AT5G43910.5 43.284 67 38 0 34 100 98 164 2.13e-15 70.1
Msa0717140 AT5G43910.1 42.647 68 39 0 34 101 98 165 2.19e-15 69.7
Msa0717140 AT5G43910.3 42.647 68 39 0 34 101 98 165 2.35e-15 69.7
Msa0717140 AT5G43910.4 43.284 67 38 0 34 100 98 164 3.87e-15 69.3
Msa0717140 AT5G43910.2 43.284 67 38 0 34 100 98 164 6.27e-15 68.9

Find 106 sgRNAs with CRISPR-Local

Find 174 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTCTTCCTTCTCCTAAAA+TGG 0.139040 5_1:+10799837 Msa0717140:five_prime_UTR
CCTCTACTTCCCTATGAAAA+AGG 0.172353 5_1:-10801440 None:intergenic
AGTTCTTTAATTCTCTTTAA+AGG 0.199445 5_1:+10802032 Msa0717140:CDS
TGAATTTGGATTCATTATTT+TGG 0.200877 5_1:-10801984 None:intergenic
ACATCAAAAGGATTTGAATT+TGG 0.215986 5_1:-10801998 None:intergenic
GGTAATTCGAAATTGTTAAT+TGG 0.229016 5_1:+10800386 Msa0717140:five_prime_UTR
TCTTTAAAGGTACTTTATTC+TGG 0.231427 5_1:+10802045 Msa0717140:CDS
TGCTTATGGCTATAAAATAT+TGG 0.261979 5_1:+10801518 Msa0717140:five_prime_UTR
GCTATATAGAATAATTAATA+TGG 0.275187 5_1:+10800975 Msa0717140:five_prime_UTR
ACCTATATAAGCCTTCTAAT+TGG 0.278035 5_1:-10801589 None:intergenic
TCTTGTTTATGATTTGCTTA+TGG 0.278177 5_1:+10801504 Msa0717140:five_prime_UTR
ATCATTATACTTTAAGTCAT+TGG 0.285182 5_1:+10801842 Msa0717140:five_prime_UTR
GTCTAGATCTCTCTTTATTT+CGG 0.290776 5_1:+10799926 Msa0717140:five_prime_UTR
ACATTATGAAACCGCCTTCT+TGG 0.294573 5_1:-10802074 None:intergenic
TTTGTAAACATTGTAATGTT+TGG 0.341945 5_1:+10800647 Msa0717140:five_prime_UTR
CTTACTTACTTATATATTCA+AGG 0.343214 5_1:+10800725 Msa0717140:five_prime_UTR
TTGGTGAAGTTAAATACAAC+TGG 0.344873 5_1:+10801537 Msa0717140:five_prime_UTR
ATTTGACAACACTGCTTTGT+TGG 0.354810 5_1:+10800562 Msa0717140:five_prime_UTR
TAAATTAGTATTAGTTAGAA+TGG 0.355383 5_1:+10801898 Msa0717140:five_prime_UTR
GACTCTACAAGTGCATAGTT+TGG 0.356752 5_1:-10801168 None:intergenic
AGGCATCCTAACATCAAAAT+AGG 0.360561 5_1:-10802976 None:intergenic
TCAATAATAATGTAGCTAAA+TGG 0.361983 5_1:-10802441 None:intergenic
ATAGCATCACATCTTATTAG+AGG 0.363100 5_1:-10800957 None:intergenic
TTTCATTTGTTCGTCATTGT+TGG 0.364427 5_1:+10800083 Msa0717140:five_prime_UTR
CACTTATAAATTTGTTTCAA+TGG 0.366587 5_1:-10800845 None:intergenic
TCTATGACTCTTGAAACTTT+TGG 0.367114 5_1:+10801727 Msa0717140:five_prime_UTR
CTTATATATTCAAGGCTTTG+AGG 0.368161 5_1:+10800733 Msa0717140:five_prime_UTR
ATCTCCCTCACGATCTTCTT+CGG 0.388938 5_1:+10799878 Msa0717140:five_prime_UTR
TATGAAGTAGCATCTATCTA+AGG 0.394509 5_1:-10801785 None:intergenic
ACACAAAAGTTGCTATACTT+TGG 0.407313 5_1:+10801392 Msa0717140:five_prime_UTR
AGGGTGTCAGAATTGAGAAT+TGG 0.408565 5_1:+10800313 Msa0717140:five_prime_UTR
TAGATAGATGCTACTTCATA+AGG 0.408832 5_1:+10801788 Msa0717140:five_prime_UTR
TCATAGGGAAGTAGAGGAGG+AGG 0.411896 5_1:+10801446 Msa0717140:five_prime_UTR
ATGCTACTTCATAAGGCAAT+AGG 0.413627 5_1:+10801795 Msa0717140:five_prime_UTR
TATGAGAAGGAAGGAGTGAT+TGG 0.416679 5_1:-10800220 None:intergenic
ATCATAATTCTAAATAGATT+CGG 0.418155 5_1:+10803066 Msa0717140:three_prime_UTR
TCACTCATGATATTTCTTGA+TGG 0.425202 5_1:+10801692 Msa0717140:five_prime_UTR
GATTTGCATTGAAAGAGATA+TGG 0.426922 5_1:+10801932 Msa0717140:five_prime_UTR
TTTGCACGCGAAAGATCTTC+CGG 0.437545 5_1:-10802921 None:intergenic
TTTCATAATGTACATGTGAT+TGG 0.438113 5_1:+10802086 Msa0717140:CDS
GCAATGACAAGAGCAGTTGC+AGG 0.440063 5_1:-10802996 None:intergenic
GAATCACTAATCCAATTAGA+AGG 0.443094 5_1:+10801578 Msa0717140:five_prime_UTR
TCCAATTAGAAGGCTTATAT+AGG 0.446485 5_1:+10801588 Msa0717140:five_prime_UTR
CACACACTAGATAGATAGTT+AGG 0.447753 5_1:-10801659 None:intergenic
TAAAGCTAACTTGCTACTAC+TGG 0.453580 5_1:-10801131 None:intergenic
ATAATGTACATGTGATTGGC+AGG 0.456154 5_1:+10802090 Msa0717140:CDS
TTATGAGCATCATATAATAA+AGG 0.456384 5_1:+10801051 Msa0717140:five_prime_UTR
AAACCTTCTCCCTCACGATC+TGG 0.459139 5_1:+10799904 Msa0717140:five_prime_UTR
AGATCTTCCGGTACCATTGT+AGG 0.463214 5_1:-10802909 None:intergenic
TGCTCTTGTCATTGCTAAAG+AGG 0.464049 5_1:+10803004 Msa0717140:CDS
GTGTCGTGTAGAATTGTTTG+TGG 0.464156 5_1:+10800494 Msa0717140:five_prime_UTR
ACAAATGAAAGATTTGTAGC+TGG 0.467034 5_1:-10800070 None:intergenic
TCTATCTAGTGTGTGTGTCT+TGG 0.481881 5_1:+10801667 Msa0717140:five_prime_UTR
TGGTGAAAATGCTTTGGTGG+TGG 0.490656 5_1:+10799961 Msa0717140:five_prime_UTR
TGCATGTTAGTGACATCAAA+AGG 0.493800 5_1:-10802010 None:intergenic
AAATTGTTATCTGCATTGAA+TGG 0.495412 5_1:+10802942 Msa0717140:CDS
GAGGATGAGTTGGTGAAAAG+AGG 0.497120 5_1:-10800146 None:intergenic
CATAGGGAAGTAGAGGAGGA+GGG 0.515518 5_1:+10801447 Msa0717140:five_prime_UTR
AATGATATCATGAGTGGTGT+TGG 0.517603 5_1:-10801825 None:intergenic
CAAATGAAAGATTTGTAGCT+GGG 0.518024 5_1:-10800069 None:intergenic
TGTTTCCAGGTCTCAAAGGA+AGG 0.519022 5_1:+10802411 Msa0717140:intron
TGCACTTGTAGAGTCAAGAT+AGG 0.519280 5_1:+10801176 Msa0717140:five_prime_UTR
TAGTCTTTCTATTGTAGACT+TGG 0.520340 5_1:+10801351 Msa0717140:five_prime_UTR
TGCATTGAAAGAGATATGGC+AGG 0.522285 5_1:+10801936 Msa0717140:five_prime_UTR
GGAGTGATTGGTGAGTGAAT+TGG 0.528420 5_1:-10800208 None:intergenic
ATATGAGAGGCTGCATTCAA+AGG 0.530565 5_1:-10801259 None:intergenic
CTTTATTTCGGTACTGTACG+TGG 0.531227 5_1:+10799938 Msa0717140:five_prime_UTR
GAAGAAGATCGTGAGGGAGA+TGG 0.533797 5_1:-10799876 None:intergenic
ATATCTTAATGTGTAAAACT+AGG 0.536246 5_1:-10801635 None:intergenic
GAGAGATGAGAGAAATAGCT+TGG 0.546216 5_1:-10800124 None:intergenic
TATTCTGGTGTATACCAAGA+AGG 0.554077 5_1:+10802060 Msa0717140:CDS
GAGGGAGATGGAAGATGTGA+AGG 0.557709 5_1:-10799864 None:intergenic
AGGATCTCCTACAATGGTAC+CGG 0.559044 5_1:+10802902 Msa0717140:CDS
CATTTGTTTCCAGGTCTCAA+AGG 0.559636 5_1:+10802407 Msa0717140:intron
TGCATTGAATGGAGCTAGAG+TGG 0.563511 5_1:+10802953 Msa0717140:CDS
GAGAGAAATAGCTTGGCCAC+TGG 0.568958 5_1:-10800117 None:intergenic
GTTTCCAGGTCTCAAAGGAA+GGG 0.571955 5_1:+10802412 Msa0717140:intron
AACAAGTAATATGAGAAGGA+AGG 0.573153 5_1:-10800229 None:intergenic
TGGTGGTGAAAATGCTTTGG+TGG 0.575117 5_1:+10799958 Msa0717140:five_prime_UTR
ACGTGGTGGTGAAAATGCTT+TGG 0.577107 5_1:+10799955 Msa0717140:five_prime_UTR
CACAGAAGGATCTCCTACAA+TGG 0.585278 5_1:+10802896 Msa0717140:CDS
AGACCAGATCGTGAGGGAGA+AGG 0.586690 5_1:-10799907 None:intergenic
GAGATCTAGACCAGATCGTG+AGG 0.594421 5_1:-10799914 None:intergenic
CTAAAACAAGTAATATGAGA+AGG 0.599237 5_1:-10800233 None:intergenic
CTATAATTCATATACTAGCA+AGG 0.611895 5_1:-10800927 None:intergenic
GCATTGAAAGAGATATGGCA+GGG 0.625573 5_1:+10801937 Msa0717140:five_prime_UTR
CGAACTTGTATATTCACAGA+AGG 0.625943 5_1:+10802882 Msa0717140:CDS
CACATGGAGATAGCATATGT+AGG 0.626011 5_1:-10800601 None:intergenic
GAACTGACATGAGGATGAGT+TGG 0.626814 5_1:-10800156 None:intergenic
AGAACATTGCACCTTGAGCA+TGG 0.627630 5_1:-10800880 None:intergenic
TTTCACCGAAGAAGATCGTG+AGG 0.640740 5_1:-10799883 None:intergenic
TAATGTACATGTGATTGGCA+GGG 0.640861 5_1:+10802091 Msa0717140:CDS
TAGAAAACAACTAGTACACA+TGG 0.641801 5_1:-10800617 None:intergenic
AAGTATAATGATATCATGAG+TGG 0.643506 5_1:-10801831 None:intergenic
TACTTCATAAGGCAATAGGA+TGG 0.650991 5_1:+10801799 Msa0717140:five_prime_UTR
AGATCTAGACCAGATCGTGA+GGG 0.657532 5_1:-10799913 None:intergenic
TATTTCGGTACTGTACGTGG+TGG 0.660901 5_1:+10799941 Msa0717140:five_prime_UTR
TCTGGTGTATACCAAGAAGG+CGG 0.666315 5_1:+10802063 Msa0717140:CDS
GTTGACTAGTACCATGCTCA+AGG 0.666739 5_1:+10800869 Msa0717140:five_prime_UTR
AGAAAGTATCACATGCTGAG+AGG 0.670021 5_1:-10801229 None:intergenic
AATTGGCTTACTCTGACAAG+AGG 0.673683 5_1:-10800191 None:intergenic
TGGTGAAGTTAAATACAACT+GGG 0.675389 5_1:+10801538 Msa0717140:five_prime_UTR
TTTAGGAGAAGGAAGAATGG+AGG 0.676317 5_1:-10799833 None:intergenic
GAGAGTCGAGAACTGACATG+AGG 0.681303 5_1:-10800165 None:intergenic
TTCACCGAAGAAGATCGTGA+GGG 0.695001 5_1:-10799882 None:intergenic
GTTGGCGAAACAATATCCAG+TGG 0.714382 5_1:+10800101 Msa0717140:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTACATTTATTCAATT+TGG + chr5_1:10801757-10801776 Msa0717140:five_prime_UTR 10.0%
!!! TTTTTACATTTATTCAATTT+GGG + chr5_1:10801758-10801777 Msa0717140:five_prime_UTR 10.0%
!! ATTTAAGAATTTGTAAAAAT+AGG - chr5_1:10801883-10801902 None:intergenic 10.0%
!! GCTATATAGAATAATTAATA+TGG + chr5_1:10800975-10800994 Msa0717140:five_prime_UTR 15.0%
!! TAAATTAGTATTAGTTAGAA+TGG + chr5_1:10801898-10801917 Msa0717140:five_prime_UTR 15.0%
!!! TTTAACTTGATCATAATTTT+TGG - chr5_1:10802613-10802632 None:intergenic 15.0%
!! ATCATAATTCTAAATAGATT+CGG + chr5_1:10803066-10803085 Msa0717140:three_prime_UTR 15.0%
!!! TTCTCATATTACTTGTTTTA+GGG + chr5_1:10800235-10800254 Msa0717140:five_prime_UTR 20.0%
!! TTTGTAAACATTGTAATGTT+TGG + chr5_1:10800647-10800666 Msa0717140:five_prime_UTR 20.0%
!! CTTACTTACTTATATATTCA+AGG + chr5_1:10800725-10800744 Msa0717140:five_prime_UTR 20.0%
!!! CACTTATAAATTTGTTTCAA+TGG - chr5_1:10800848-10800867 None:intergenic 20.0%
!! TTATGAGCATCATATAATAA+AGG + chr5_1:10801051-10801070 Msa0717140:five_prime_UTR 20.0%
!! ATATCTTAATGTGTAAAACT+AGG - chr5_1:10801638-10801657 None:intergenic 20.0%
!! ATCATTATACTTTAAGTCAT+TGG + chr5_1:10801842-10801861 Msa0717140:five_prime_UTR 20.0%
!!! TGAATTTGGATTCATTATTT+TGG - chr5_1:10801987-10802006 None:intergenic 20.0%
!! AGTTCTTTAATTCTCTTTAA+AGG + chr5_1:10802032-10802051 Msa0717140:CDS 20.0%
!! TCAATAATAATGTAGCTAAA+TGG - chr5_1:10802444-10802463 None:intergenic 20.0%
!! ATGTAAAATCATTTAAGCAA+TGG - chr5_1:10802483-10802502 None:intergenic 20.0%
!! TACAAGTATATGAAAGTTAT+TGG + chr5_1:10802576-10802595 Msa0717140:intron 20.0%
! CTAAAACAAGTAATATGAGA+AGG - chr5_1:10800236-10800255 None:intergenic 25.0%
!!! CTTCTCATATTACTTGTTTT+AGG + chr5_1:10800234-10800253 Msa0717140:five_prime_UTR 25.0%
!! TGAGAATTGGAAATTGTTTT+AGG + chr5_1:10800267-10800286 Msa0717140:five_prime_UTR 25.0%
!!! TTGTTTTAGGATTTCAGAAT+TGG + chr5_1:10800280-10800299 Msa0717140:five_prime_UTR 25.0%
!!! TGTTTTAGGATTTCAGAATT+GGG + chr5_1:10800281-10800300 Msa0717140:five_prime_UTR 25.0%
!! TGAGAATTGGAAATTGTTTT+AGG + chr5_1:10800267-10800286 Msa0717140:five_prime_UTR 25.0%
!!! GAGAATTGGAAATTGTTTTA+GGG + chr5_1:10800268-10800287 Msa0717140:five_prime_UTR 25.0%
!!! TTGTTTTAGGGTTTTAGAAT+TGG + chr5_1:10800247-10800266 Msa0717140:five_prime_UTR 25.0%
!!! TGTTTTAGGGTTTTAGAATT+GGG + chr5_1:10800248-10800267 Msa0717140:five_prime_UTR 25.0%
! GGTAATTCGAAATTGTTAAT+TGG + chr5_1:10800386-10800405 Msa0717140:five_prime_UTR 25.0%
!!! TCCTTGCTTTTTTTCTAATT+TGG + chr5_1:10800814-10800833 Msa0717140:five_prime_UTR 25.0%
!!! CCTTGCTTTTTTTCTAATTT+GGG + chr5_1:10800815-10800834 Msa0717140:five_prime_UTR 25.0%
! CTATAATTCATATACTAGCA+AGG - chr5_1:10800930-10800949 None:intergenic 25.0%
!!! TTTTTCTATGCTTTGTTTAC+AGG + chr5_1:10801027-10801046 Msa0717140:five_prime_UTR 25.0%
!!! TTTTCTATGCTTTGTTTACA+GGG + chr5_1:10801028-10801047 Msa0717140:five_prime_UTR 25.0%
!!! AGCCTCTTTTTAAAGATAAT+GGG + chr5_1:10801089-10801108 Msa0717140:five_prime_UTR 25.0%
!! TTCCCATTATCTTTAAAAAG+AGG - chr5_1:10801094-10801113 None:intergenic 25.0%
!!! TCTTGTTTATGATTTGCTTA+TGG + chr5_1:10801504-10801523 Msa0717140:five_prime_UTR 25.0%
! TGCTTATGGCTATAAAATAT+TGG + chr5_1:10801518-10801537 Msa0717140:five_prime_UTR 25.0%
! AAGTATAATGATATCATGAG+TGG - chr5_1:10801834-10801853 None:intergenic 25.0%
!!! TTTTGGTAGTTCTACTAAAT+TGG - chr5_1:10801970-10801989 None:intergenic 25.0%
! ACATCAAAAGGATTTGAATT+TGG - chr5_1:10802001-10802020 None:intergenic 25.0%
!!! TCTTTAAAGGTACTTTATTC+TGG + chr5_1:10802045-10802064 Msa0717140:CDS 25.0%
! TTTCATAATGTACATGTGAT+TGG + chr5_1:10802086-10802105 Msa0717140:CDS 25.0%
! AAATAGCAAGTTTATGATGT+TGG - chr5_1:10802290-10802309 None:intergenic 25.0%
! TCAAGTTAAATTTAAGCACT+AGG + chr5_1:10802623-10802642 Msa0717140:intron 25.0%
! AAATTGTTATCTGCATTGAA+TGG + chr5_1:10802942-10802961 Msa0717140:CDS 25.0%
! ATGTGAAGGTTTTCCATTTT+AGG - chr5_1:10799853-10799872 None:intergenic 30.0%
! GTCTAGATCTCTCTTTATTT+CGG + chr5_1:10799926-10799945 Msa0717140:five_prime_UTR 30.0%
CAAATGAAAGATTTGTAGCT+GGG - chr5_1:10800072-10800091 None:intergenic 30.0%
ACAAATGAAAGATTTGTAGC+TGG - chr5_1:10800073-10800092 None:intergenic 30.0%
TTTCATTTGTTCGTCATTGT+TGG + chr5_1:10800083-10800102 Msa0717140:five_prime_UTR 30.0%
AACAAGTAATATGAGAAGGA+AGG - chr5_1:10800232-10800251 None:intergenic 30.0%
!!! AGGGTTTTAGAATTGAGAAT+TGG + chr5_1:10800254-10800273 Msa0717140:five_prime_UTR 30.0%
!!! GTTTTAGGATTTCAGAATTG+GGG + chr5_1:10800282-10800301 Msa0717140:five_prime_UTR 30.0%
!!! GTTTTAGGGTTTTAGAATTG+GGG + chr5_1:10800249-10800268 Msa0717140:five_prime_UTR 30.0%
! TTAGAATTGGGGATTGTTTT+AGG + chr5_1:10800352-10800371 Msa0717140:five_prime_UTR 30.0%
!! TAGAATTGGGGATTGTTTTA+GGG + chr5_1:10800353-10800372 Msa0717140:five_prime_UTR 30.0%
TAGAAAACAACTAGTACACA+TGG - chr5_1:10800620-10800639 None:intergenic 30.0%
CTTATATATTCAAGGCTTTG+AGG + chr5_1:10800733-10800752 Msa0717140:five_prime_UTR 30.0%
CCCAAATTAGAAAAAAAGCA+AGG - chr5_1:10800818-10800837 None:intergenic 30.0%
!!! GCTTTTTTTCTAATTTGGGT+CGG + chr5_1:10800819-10800838 Msa0717140:five_prime_UTR 30.0%
ATAGCATCACATCTTATTAG+AGG - chr5_1:10800960-10800979 None:intergenic 30.0%
!! GAGCCTCTTTTTAAAGATAA+TGG + chr5_1:10801088-10801107 Msa0717140:five_prime_UTR 30.0%
! ATGACATTTTCTCATATGAG+AGG - chr5_1:10801275-10801294 None:intergenic 30.0%
TAGTCTTTCTATTGTAGACT+TGG + chr5_1:10801351-10801370 Msa0717140:five_prime_UTR 30.0%
! AAGTATAGCAACTTTTGTGT+TGG - chr5_1:10801392-10801411 None:intergenic 30.0%
ACACAAAAGTTGCTATACTT+TGG + chr5_1:10801392-10801411 Msa0717140:five_prime_UTR 30.0%
!! ATACACTTCCCTTTTTCATA+GGG + chr5_1:10801431-10801450 Msa0717140:five_prime_UTR 30.0%
TTGGTGAAGTTAAATACAAC+TGG + chr5_1:10801537-10801556 Msa0717140:five_prime_UTR 30.0%
TGGTGAAGTTAAATACAACT+GGG + chr5_1:10801538-10801557 Msa0717140:five_prime_UTR 30.0%
GAATCACTAATCCAATTAGA+AGG + chr5_1:10801578-10801597 Msa0717140:five_prime_UTR 30.0%
TCCAATTAGAAGGCTTATAT+AGG + chr5_1:10801588-10801607 Msa0717140:five_prime_UTR 30.0%
ACCTATATAAGCCTTCTAAT+TGG - chr5_1:10801592-10801611 None:intergenic 30.0%
TCACTCATGATATTTCTTGA+TGG + chr5_1:10801692-10801711 Msa0717140:five_prime_UTR 30.0%
! TCTATGACTCTTGAAACTTT+TGG + chr5_1:10801727-10801746 Msa0717140:five_prime_UTR 30.0%
TATGAAGTAGCATCTATCTA+AGG - chr5_1:10801788-10801807 None:intergenic 30.0%
! TAGATAGATGCTACTTCATA+AGG + chr5_1:10801788-10801807 Msa0717140:five_prime_UTR 30.0%
GATTTGCATTGAAAGAGATA+TGG + chr5_1:10801932-10801951 Msa0717140:five_prime_UTR 30.0%
!! AAGCACTAGGAGTATTAAAA+AGG + chr5_1:10802636-10802655 Msa0717140:intron 30.0%
AGACATGCTTGATAGATAAT+TGG + chr5_1:10802692-10802711 Msa0717140:intron 30.0%
AATTGGATTTCTTAAGCATG+TGG + chr5_1:10802709-10802728 Msa0717140:intron 30.0%
! TAAGCATGTGGTTTGATAAT+CGG + chr5_1:10802721-10802740 Msa0717140:intron 30.0%
TCCTATAATGTTGAACATTC+AGG + chr5_1:10802853-10802872 Msa0717140:intron 30.0%
TCCTGAATGTTCAACATTAT+AGG - chr5_1:10802857-10802876 None:intergenic 30.0%
! CTATTAGTACTTTTCTCAAG+AGG + chr5_1:10803112-10803131 Msa0717140:three_prime_UTR 30.0%
!! CATTTTAGGAGAAGGAAGAA+TGG - chr5_1:10799839-10799858 None:intergenic 35.0%
CATTCTTCCTTCTCCTAAAA+TGG + chr5_1:10799837-10799856 Msa0717140:five_prime_UTR 35.0%
!! AGGTTTTCCATTTTAGGAGA+AGG - chr5_1:10799847-10799866 None:intergenic 35.0%
! TCAGAATTGGGGATTGTTTT+AGG + chr5_1:10800293-10800312 Msa0717140:five_prime_UTR 35.0%
!! CAGAATTGGGGATTGTTTTA+GGG + chr5_1:10800294-10800313 Msa0717140:five_prime_UTR 35.0%
!!! TTGTTTTAGGGTGTCAGAAT+TGG + chr5_1:10800306-10800325 Msa0717140:five_prime_UTR 35.0%
ATTTGACAACACTGCTTTGT+TGG + chr5_1:10800562-10800581 Msa0717140:five_prime_UTR 35.0%
!! TAAAGCTAACTTGCTACTAC+TGG - chr5_1:10801134-10801153 None:intergenic 35.0%
!! GATACACTTCCCTTTTTCAT+AGG + chr5_1:10801430-10801449 Msa0717140:five_prime_UTR 35.0%
CTCTACTTCCCTATGAAAAA+GGG - chr5_1:10801442-10801461 None:intergenic 35.0%
CACACACTAGATAGATAGTT+AGG - chr5_1:10801662-10801681 None:intergenic 35.0%
! ATGCTACTTCATAAGGCAAT+AGG + chr5_1:10801795-10801814 Msa0717140:five_prime_UTR 35.0%
TACTTCATAAGGCAATAGGA+TGG + chr5_1:10801799-10801818 Msa0717140:five_prime_UTR 35.0%
AATGATATCATGAGTGGTGT+TGG - chr5_1:10801828-10801847 None:intergenic 35.0%
TGCATGTTAGTGACATCAAA+AGG - chr5_1:10802013-10802032 None:intergenic 35.0%
TATTCTGGTGTATACCAAGA+AGG + chr5_1:10802060-10802079 Msa0717140:CDS 35.0%
ATAATGTACATGTGATTGGC+AGG + chr5_1:10802090-10802109 Msa0717140:CDS 35.0%
TAATGTACATGTGATTGGCA+GGG + chr5_1:10802091-10802110 Msa0717140:CDS 35.0%
GGACCTTACATATTCACTTT+AGG + chr5_1:10802123-10802142 Msa0717140:intron 35.0%
GTGCCTAAAGTGAATATGTA+AGG - chr5_1:10802129-10802148 None:intergenic 35.0%
TTCCTTTCTCATTTGTTTCC+AGG + chr5_1:10802398-10802417 Msa0717140:intron 35.0%
CAAGTGAAAAATTAGGAGGA+AGG - chr5_1:10802525-10802544 None:intergenic 35.0%
! TCCTCCTAATTTTTCACTTG+TGG + chr5_1:10802525-10802544 Msa0717140:intron 35.0%
! CCTCCTAATTTTTCACTTGT+GGG + chr5_1:10802526-10802545 Msa0717140:intron 35.0%
! CTCCTAATTTTTCACTTGTG+GGG + chr5_1:10802527-10802546 Msa0717140:intron 35.0%
GACAGTAGACACTATGTTAT+TGG - chr5_1:10802667-10802686 None:intergenic 35.0%
!! CAACTGCTAATTTTGACACA+TGG + chr5_1:10802789-10802808 Msa0717140:intron 35.0%
CGAACTTGTATATTCACAGA+AGG + chr5_1:10802882-10802901 Msa0717140:CDS 35.0%
AGGCATCCTAACATCAAAAT+AGG - chr5_1:10802979-10802998 None:intergenic 35.0%
! CTTTATTTCGGTACTGTACG+TGG + chr5_1:10799938-10799957 Msa0717140:five_prime_UTR 40.0%
! CTCTCTCTCTCTCTTCATTT+TGG + chr5_1:10800033-10800052 Msa0717140:five_prime_UTR 40.0%
GAGAGATGAGAGAAATAGCT+TGG - chr5_1:10800127-10800146 None:intergenic 40.0%
! AATTGGCTTACTCTGACAAG+AGG - chr5_1:10800194-10800213 None:intergenic 40.0%
TATGAGAAGGAAGGAGTGAT+TGG - chr5_1:10800223-10800242 None:intergenic 40.0%
! AGGGTGTCAGAATTGAGAAT+TGG + chr5_1:10800313-10800332 Msa0717140:five_prime_UTR 40.0%
GTGTCGTGTAGAATTGTTTG+TGG + chr5_1:10800494-10800513 Msa0717140:five_prime_UTR 40.0%
CACATGGAGATAGCATATGT+AGG - chr5_1:10800604-10800623 None:intergenic 40.0%
GACTCTACAAGTGCATAGTT+TGG - chr5_1:10801171-10801190 None:intergenic 40.0%
TGCACTTGTAGAGTCAAGAT+AGG + chr5_1:10801176-10801195 Msa0717140:five_prime_UTR 40.0%
!! AGAAAGTATCACATGCTGAG+AGG - chr5_1:10801232-10801251 None:intergenic 40.0%
ATATGAGAGGCTGCATTCAA+AGG - chr5_1:10801262-10801281 None:intergenic 40.0%
CCTCTACTTCCCTATGAAAA+AGG - chr5_1:10801443-10801462 None:intergenic 40.0%
!! CCTTTTTCATAGGGAAGTAG+AGG + chr5_1:10801440-10801459 Msa0717140:five_prime_UTR 40.0%
! TTTTCATAGGGAAGTAGAGG+AGG + chr5_1:10801443-10801462 Msa0717140:five_prime_UTR 40.0%
TCTATCTAGTGTGTGTGTCT+TGG + chr5_1:10801667-10801686 Msa0717140:five_prime_UTR 40.0%
TGCATTGAAAGAGATATGGC+AGG + chr5_1:10801936-10801955 Msa0717140:five_prime_UTR 40.0%
GCATTGAAAGAGATATGGCA+GGG + chr5_1:10801937-10801956 Msa0717140:five_prime_UTR 40.0%
ACATTATGAAACCGCCTTCT+TGG - chr5_1:10802077-10802096 None:intergenic 40.0%
! TGATTGGCAGGGTATCATAA+TGG + chr5_1:10802102-10802121 Msa0717140:intron 40.0%
GACCTGGAAACAAATGAGAA+AGG - chr5_1:10802403-10802422 None:intergenic 40.0%
CATTTGTTTCCAGGTCTCAA+AGG + chr5_1:10802407-10802426 Msa0717140:intron 40.0%
TAAGCAATGGTACATACGTG+TGG - chr5_1:10802470-10802489 None:intergenic 40.0%
CCCACAAGTGAAAAATTAGG+AGG - chr5_1:10802529-10802548 None:intergenic 40.0%
AGCCCCACAAGTGAAAAATT+AGG - chr5_1:10802532-10802551 None:intergenic 40.0%
!!! GAGTGGCCTATTTTGATGTT+AGG + chr5_1:10802970-10802989 Msa0717140:CDS 40.0%
TGCTCTTGTCATTGCTAAAG+AGG + chr5_1:10803004-10803023 Msa0717140:CDS 40.0%
ATCTCCCTCACGATCTTCTT+CGG + chr5_1:10799878-10799897 Msa0717140:five_prime_UTR 45.0%
TTCACCGAAGAAGATCGTGA+GGG - chr5_1:10799885-10799904 None:intergenic 45.0%
TTTCACCGAAGAAGATCGTG+AGG - chr5_1:10799886-10799905 None:intergenic 45.0%
AGATCTAGACCAGATCGTGA+GGG - chr5_1:10799916-10799935 None:intergenic 45.0%
TATTTCGGTACTGTACGTGG+TGG + chr5_1:10799941-10799960 Msa0717140:five_prime_UTR 45.0%
! ACGTGGTGGTGAAAATGCTT+TGG + chr5_1:10799955-10799974 Msa0717140:five_prime_UTR 45.0%
! TGGTGGTGAAAATGCTTTGG+TGG + chr5_1:10799958-10799977 Msa0717140:five_prime_UTR 45.0%
TGGTGAAAATGCTTTGGTGG+TGG + chr5_1:10799961-10799980 Msa0717140:five_prime_UTR 45.0%
GTTGGCGAAACAATATCCAG+TGG + chr5_1:10800101-10800120 Msa0717140:five_prime_UTR 45.0%
!! GAGGATGAGTTGGTGAAAAG+AGG - chr5_1:10800149-10800168 None:intergenic 45.0%
GAACTGACATGAGGATGAGT+TGG - chr5_1:10800159-10800178 None:intergenic 45.0%
GGAGTGATTGGTGAGTGAAT+TGG - chr5_1:10800211-10800230 None:intergenic 45.0%
GTTGACTAGTACCATGCTCA+AGG + chr5_1:10800869-10800888 Msa0717140:five_prime_UTR 45.0%
AGAACATTGCACCTTGAGCA+TGG - chr5_1:10800883-10800902 None:intergenic 45.0%
TCTGGTGTATACCAAGAAGG+CGG + chr5_1:10802063-10802082 Msa0717140:CDS 45.0%
TGTTTCCAGGTCTCAAAGGA+AGG + chr5_1:10802411-10802430 Msa0717140:intron 45.0%
GTTTCCAGGTCTCAAAGGAA+GGG + chr5_1:10802412-10802431 Msa0717140:intron 45.0%
CACAGAAGGATCTCCTACAA+TGG + chr5_1:10802896-10802915 Msa0717140:CDS 45.0%
AGGATCTCCTACAATGGTAC+CGG + chr5_1:10802902-10802921 Msa0717140:CDS 45.0%
AGATCTTCCGGTACCATTGT+AGG - chr5_1:10802912-10802931 None:intergenic 45.0%
TTTGCACGCGAAAGATCTTC+CGG - chr5_1:10802924-10802943 None:intergenic 45.0%
TGCATTGAATGGAGCTAGAG+TGG + chr5_1:10802953-10802972 Msa0717140:CDS 45.0%
GAGGGAGATGGAAGATGTGA+AGG - chr5_1:10799867-10799886 None:intergenic 50.0%
GAAGAAGATCGTGAGGGAGA+TGG - chr5_1:10799879-10799898 None:intergenic 50.0%
AAACCTTCTCCCTCACGATC+TGG + chr5_1:10799904-10799923 Msa0717140:five_prime_UTR 50.0%
GAGATCTAGACCAGATCGTG+AGG - chr5_1:10799917-10799936 None:intergenic 50.0%
GAGAGAAATAGCTTGGCCAC+TGG - chr5_1:10800120-10800139 None:intergenic 50.0%
GAGAGTCGAGAACTGACATG+AGG - chr5_1:10800168-10800187 None:intergenic 50.0%
TCATAGGGAAGTAGAGGAGG+AGG + chr5_1:10801446-10801465 Msa0717140:five_prime_UTR 50.0%
CATAGGGAAGTAGAGGAGGA+GGG + chr5_1:10801447-10801466 Msa0717140:five_prime_UTR 50.0%
!! TAGTCCCTTCCTTTGAGACC+TGG - chr5_1:10802419-10802438 None:intergenic 50.0%
GCAATGACAAGAGCAGTTGC+AGG - chr5_1:10802999-10803018 None:intergenic 50.0%
AGACCAGATCGTGAGGGAGA+AGG - chr5_1:10799910-10799929 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 10799834 10803153 10799834 ID=Msa0717140;Name=Msa0717140
chr5_1 mRNA 10799834 10803153 10799834 ID=Msa0717140-mRNA-1;Parent=Msa0717140;Name=Msa0717140-mRNA-1;_AED=0.04;_eAED=0.04;_QI=2183|1|1|1|0.5|0.66|3|113|104
chr5_1 exon 10799834 10802112 10799834 ID=Msa0717140-mRNA-1:exon:4407;Parent=Msa0717140-mRNA-1
chr5_1 exon 10802420 10802472 10802420 ID=Msa0717140-mRNA-1:exon:4408;Parent=Msa0717140-mRNA-1
chr5_1 exon 10802875 10803153 10802875 ID=Msa0717140-mRNA-1:exon:4409;Parent=Msa0717140-mRNA-1
chr5_1 five_prime_UTR 10799834 10802016 10799834 ID=Msa0717140-mRNA-1:five_prime_utr;Parent=Msa0717140-mRNA-1
chr5_1 CDS 10802017 10802112 10802017 ID=Msa0717140-mRNA-1:cds;Parent=Msa0717140-mRNA-1
chr5_1 CDS 10802420 10802472 10802420 ID=Msa0717140-mRNA-1:cds;Parent=Msa0717140-mRNA-1
chr5_1 CDS 10802875 10803040 10802875 ID=Msa0717140-mRNA-1:cds;Parent=Msa0717140-mRNA-1
chr5_1 three_prime_UTR 10803041 10803153 10803041 ID=Msa0717140-mRNA-1:three_prime_utr;Parent=Msa0717140-mRNA-1
Gene Sequence

>Msa0717140

ATGTCACTAACATGCAGTTCTTTAATTCTCTTTAAAGGTACTTTATTCTGGTGTATACCAAGAAGGCGGTTTCATAATGTACATGTGATTGGCAGGGTCTCAAAGGAAGGGACTACACCATTTAGCTACATTATTATTGACAACCACACGAAGACACGAACTTGTATATTCACAGAAGGATCTCCTACAATGGTACCGGAAGATCTTTCGCGTGCAAAATTGTTATCTGCATTGAATGGAGCTAGAGTGGCCTATTTTGATGTTAGGATGCCTGCAACTGCTCTTGTCATTGCTAAAGAGGTAATTAGATATTAG
Protein sequence

>Msa0717140

MSLTCSSLILFKGTLFWCIPRRRFHNVHVIGRVSKEGTTPFSYIIIDNHTKTRTCIFTEGSPTMVPEDLSRAKLLSALNGARVAYFDVRMPATALVIAKEVIRY*