AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0719210

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0719210 MtrunA17_Chr5g0409641 97.428 311 8 0 1 311 1 311 0.0 633
Msa0719210 MtrunA17_Chr5g0409651 93.569 311 20 0 1 311 1 311 0.0 611
Msa0719210 MtrunA17_Chr8g0369391 75.241 311 76 1 1 310 1 311 1.11e-178 496
Msa0719210 MtrunA17_Chr3g0141691 47.557 307 153 3 3 307 4 304 9.69e-95 283
Msa0719210 MtrunA17_Chr5g0404471 47.742 310 154 3 3 310 5 308 3.39e-94 281
Msa0719210 MtrunA17_Chr3g0141681 47.249 309 153 4 3 307 4 306 1.10e-92 277
Msa0719210 MtrunA17_Chr3g0144701 47.249 309 155 4 4 310 9 311 2.98e-90 271
Msa0719210 MtrunA17_Chr5g0404481 42.056 321 166 5 3 310 4 317 1.74e-79 244
Msa0719210 MtrunA17_Chr5g0404511 42.056 321 166 5 3 310 4 317 2.44e-79 244
Msa0719210 MtrunA17_Chr4g0035131 42.997 307 167 3 4 308 45 345 6.81e-79 244
Msa0719210 MtrunA17_Chr3g0078901 42.628 312 159 8 2 307 4 301 7.40e-77 237
Msa0719210 MtrunA17_Chr4g0035121 41.290 310 174 3 1 307 1 305 1.46e-75 234
Msa0719210 MtrunA17_Chr4g0035111 39.482 309 179 3 2 308 5 307 5.06e-74 230
Msa0719210 MtrunA17_Chr3g0078831 41.935 310 164 7 2 307 4 301 6.59e-72 224
Msa0719210 MtrunA17_Chr1g0198771 33.016 315 198 6 1 310 11 317 4.07e-56 185
Msa0719210 MtrunA17_Chr3g0078941 37.931 203 116 5 108 307 12 207 4.20e-39 137
Msa0719210 MtrunA17_Chr3g0078951 43.119 109 53 3 3 110 5 105 1.62e-18 80.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0719210 AT1G32100.1 70.130 308 92 0 3 310 9 316 8.89e-172 479
Msa0719210 AT4G13660.1 68.831 308 96 0 3 310 9 316 9.32e-165 461
Msa0719210 AT4G13660.2 68.831 308 96 0 3 310 9 316 9.32e-165 461
Msa0719210 AT1G75290.1 45.687 313 160 4 3 311 5 311 5.03e-90 271
Msa0719210 AT1G75290.2 45.687 313 160 4 3 311 32 338 1.96e-89 271
Msa0719210 AT1G75280.1 45.833 312 160 4 3 311 5 310 1.89e-88 267
Msa0719210 AT4G39230.1 44.695 311 162 4 3 310 4 307 3.42e-84 256
Msa0719210 AT1G75300.1 44.037 327 154 7 3 310 5 321 1.81e-83 254
Msa0719210 AT1G19540.1 42.765 311 170 4 4 310 3 309 3.50e-77 238
Msa0719210 AT1G19540.2 44.574 258 137 3 4 259 3 256 6.97e-69 215
Msa0719210 AT4G34540.1 38.782 312 168 5 3 307 7 302 2.55e-66 210

Find 73 sgRNAs with CRISPR-Local

Find 176 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGTTTCCTACCTTCAGAATT+TGG 0.123553 5_1:-14199970 Msa0719210:CDS
TGGAATGGACCCAGCTCTTA+TGG 0.218113 5_1:-14199950 Msa0719210:CDS
AGTTTAATTTATCCTCAATA+TGG 0.251134 5_1:+14201636 None:intergenic
GACGTTCCCTGCATCCTTGA+TGG 0.252714 5_1:+14201283 None:intergenic
GAGAAATGGGAGAAACTTAT+TGG 0.253360 5_1:-14199476 Msa0719210:CDS
TCATCAAATGTGACTCTTCC+TGG 0.258900 5_1:+14199913 None:intergenic
TCAGTTTCAAGCCCTATATC+TGG 0.304317 5_1:+14201482 None:intergenic
GTACTTCAACGTCCAGATAT+AGG 0.328641 5_1:-14201494 Msa0719210:CDS
AAGAAAGTCTTGTTCGGATA+TGG 0.342755 5_1:+14199433 None:intergenic
AAAGAGAGCTAGTTGAGAAA+TGG 0.344521 5_1:-14199490 Msa0719210:CDS
TACTTCAACGTCCAGATATA+GGG 0.350833 5_1:-14201493 Msa0719210:CDS
TTGATGAGAAAATGACTATA+AGG 0.356440 5_1:-14199897 Msa0719210:CDS
TTTCTCTAATCATCAAAGTT+TGG 0.357986 5_1:-14201402 Msa0719210:CDS
AAGAGAGCTAGTTGAGAAAT+GGG 0.369973 5_1:-14199489 Msa0719210:CDS
CGAGACAAGGTTGTTCTCTA+TGG 0.382332 5_1:-14199769 Msa0719210:CDS
TGTTTCGCGGCCTACTTTGC+CGG 0.387206 5_1:-14199826 Msa0719210:CDS
GGAATGGACCCAGCTCTTAT+GGG 0.395550 5_1:-14199949 Msa0719210:CDS
GTTGTTATTTGCACCATGTC+TGG 0.405325 5_1:-14201356 Msa0719210:CDS
TGCCGGTAACCTCTCTCAAA+TGG 0.408456 5_1:-14199809 Msa0719210:CDS
TGAACAAGACAATATATCTA+AGG 0.409071 5_1:-14199535 Msa0719210:CDS
TTGAGGATAAATTAAACTAT+AGG 0.412531 5_1:-14201631 Msa0719210:five_prime_UTR
CTTCTTTCATTCAAGAAACT+AGG 0.440060 5_1:-14201446 Msa0719210:CDS
AGAAATGCCCCACTCCTACT+TGG 0.468869 5_1:+14199225 None:intergenic
TCCCATTTGAGAGAGGTTAC+CGG 0.471634 5_1:+14199807 None:intergenic
ATGCGTGTGTATTGCACCTC+TGG 0.472227 5_1:+14199131 None:intergenic
CCACTCCTACTTGGCTTGCA+AGG 0.479291 5_1:+14199234 None:intergenic
GCCGGTAACCTCTCTCAAAT+GGG 0.483634 5_1:-14199808 Msa0719210:CDS
ATTGTCTTGTTCAATGTTCG+AGG 0.498830 5_1:+14199545 None:intergenic
AGAGAGGTTACCGGCAAAGT+AGG 0.504148 5_1:+14199816 None:intergenic
GGAAGAAAGAAAGTCTTGTT+CGG 0.504559 5_1:+14199427 None:intergenic
ATTGAGGCCATCAAGGATGC+AGG 0.506162 5_1:-14201290 Msa0719210:CDS
CAAACTTATTGAGGCCATCA+AGG 0.506330 5_1:-14201297 Msa0719210:CDS
CATCTCCGCCAACTGTTTCG+CGG 0.506412 5_1:-14199839 Msa0719210:CDS
AGTGCATGTCCCATAAGAGC+TGG 0.509909 5_1:+14199940 None:intergenic
GACTTTCTTTCTTCCATGAA+AGG 0.513933 5_1:-14199419 Msa0719210:intron
AAAGTAGGCCGCGAAACAGT+TGG 0.525770 5_1:+14199831 None:intergenic
CCTACCTTCAGAATTTGGAA+TGG 0.527632 5_1:-14199965 Msa0719210:CDS
AACATTAATGGGAAAGAGCA+AGG 0.531316 5_1:-14201594 Msa0719210:exon
GACACATGATATCCATATTG+AGG 0.535746 5_1:-14201648 Msa0719210:five_prime_UTR
TTGTCTTGTTCAATGTTCGA+GGG 0.543328 5_1:+14199546 None:intergenic
GACTATAAGGAAAACAATTG+AGG 0.545612 5_1:-14199884 Msa0719210:CDS
GATGTAAGTGAAAGGAATGT+TGG 0.548414 5_1:+14199858 None:intergenic
GAAACTAGGTGCTCATCTTG+TGG 0.554098 5_1:-14201432 Msa0719210:CDS
GATGCAACTCAAACTTATTG+AGG 0.556594 5_1:-14201306 Msa0719210:CDS
TTGGACCTTGCAAGCCAAGT+AGG 0.556786 5_1:-14199239 Msa0719210:CDS
ACTCTTTCCTCCACGAGACA+AGG 0.562903 5_1:-14199782 Msa0719210:CDS
GGATATCATGTGTCAAGCTA+AGG 0.570190 5_1:+14201657 None:intergenic
TATGGCGACGGCAATGTCAA+AGG 0.572714 5_1:-14199751 Msa0719210:intron
ATGGGACATGCACTTGAACC+AGG 0.574655 5_1:-14199931 Msa0719210:CDS
CCTTGCAAGCCAAGTAGGAG+TGG 0.574853 5_1:-14199234 Msa0719210:CDS
TGAAATACGTGATGGTGAAG+AGG 0.584479 5_1:-14199168 Msa0719210:CDS
CTTGCAAGCCAAGTAGGAGT+GGG 0.587692 5_1:-14199233 Msa0719210:CDS
ATCTGGACGTTGAAGTACAT+AGG 0.593407 5_1:+14201499 None:intergenic
CAACTAGCTCTCTTTGAGTG+AGG 0.596234 5_1:+14199498 None:intergenic
GGGGTACTGGGTATATTGGT+AGG 0.599217 5_1:-14201559 Msa0719210:CDS
CTAGGTGCTCATCTTGTGGA+AGG 0.605859 5_1:-14201428 Msa0719210:CDS
GGGTCCATTCCAAATTCTGA+AGG 0.606637 5_1:+14199961 None:intergenic
CCATTCCAAATTCTGAAGGT+AGG 0.607236 5_1:+14199965 None:intergenic
GTGCATGTCCCATAAGAGCT+GGG 0.609221 5_1:+14199941 None:intergenic
TAGAGAACAACCTTGTCTCG+TGG 0.612182 5_1:+14199772 None:intergenic
TCGAAAATGAACTCCAGACA+TGG 0.615339 5_1:+14201343 None:intergenic
AAGGTTGTTCTCTATGGCGA+CGG 0.618470 5_1:-14199763 Msa0719210:CDS
GGCATCAAAGCTTTATCCAG+AGG 0.622118 5_1:-14199147 Msa0719210:CDS
AGAGTGTTCCCATTTGAGAG+AGG 0.622439 5_1:+14199800 None:intergenic
AGAGGTGCAATACACACGCA+TGG 0.626180 5_1:-14199129 Msa0719210:CDS
TATTGGTAGGAGAATAGTGA+AGG 0.627838 5_1:-14201546 Msa0719210:CDS
TTGGCGGAGATGTAAGTGAA+AGG 0.629490 5_1:+14199850 None:intergenic
TTGAGGCCATCAAGGATGCA+GGG 0.635532 5_1:-14201289 Msa0719210:CDS
GTGAAGGCAAGTTTAGAACA+AGG 0.641128 5_1:-14201530 Msa0719210:CDS
CAAGGATGCAGGGAACGTCA+AGG 0.642191 5_1:-14201279 Msa0719210:intron
GTAGGCCGCGAAACAGTTGG+CGG 0.669276 5_1:+14199834 None:intergenic
TTGCAAGCCAAGTAGGAGTG+GGG 0.683571 5_1:-14199232 Msa0719210:CDS
AGAACAACCTTGTCTCGTGG+AGG 0.687370 5_1:+14199775 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATACAAATATCAATAATTAT+TGG - chr5_1:14199426-14199445 Msa0719210:CDS 10.0%
!! ATATAATAATATGTTTAGAA+AGG + chr5_1:14199597-14199616 None:intergenic 10.0%
!! TTTCTAAACATATTATTATA+TGG - chr5_1:14199596-14199615 Msa0719210:CDS 10.0%
!!! TTTATTAAAATTTCTAGAAA+TGG - chr5_1:14200396-14200415 Msa0719210:intron 10.0%
!!! CAATGTATATTTTTTATTAT+TGG + chr5_1:14201647-14201666 None:intergenic 10.0%
!! CAATAATAAAAAATATACAT+TGG - chr5_1:14201645-14201664 Msa0719210:five_prime_UTR 10.0%
!! ATAATAAAAAATATACATTG+GGG - chr5_1:14201647-14201666 Msa0719210:five_prime_UTR 10.0%
!! TACGTATATATAATTAAGAT+AGG + chr5_1:14199705-14199724 None:intergenic 15.0%
!! AAGTATTATATGTATTGAAT+GGG - chr5_1:14199967-14199986 Msa0719210:CDS 15.0%
!! ACTAGTTAATGAATTATAAA+AGG + chr5_1:14200169-14200188 None:intergenic 15.0%
!!! AATAAATACCTCTAAAAAAA+TGG - chr5_1:14200297-14200316 Msa0719210:intron 15.0%
!!! TTTTTATACCATTTTTTTAG+AGG + chr5_1:14200308-14200327 None:intergenic 15.0%
!!! AATGTTAACTATTTTTTCTA+AGG + chr5_1:14200943-14200962 None:intergenic 15.0%
!!! ATAATTTTATTTTGGGTAAA+GGG + chr5_1:14201275-14201294 None:intergenic 15.0%
!!! ATTTTAGCTATTTATTTTCT+TGG + chr5_1:14201303-14201322 None:intergenic 15.0%
!!! ACTCTTCTTTTTATTTTTTT+AGG - chr5_1:14201359-14201378 Msa0719210:CDS 15.0%
!!! AAATTTGTACTTTTTTTAAG+AGG - chr5_1:14201598-14201617 Msa0719210:exon 15.0%
!!! GTTTAATGAAATCATTTTTT+GGG + chr5_1:14201715-14201734 None:intergenic 15.0%
!!! TGTTTAATGAAATCATTTTT+TGG + chr5_1:14201716-14201735 None:intergenic 15.0%
!!! ACATCCTTTTTTTTTTTTTG+AGG - chr5_1:14198943-14198962 Msa0719210:three_prime_UTR 20.0%
!! TTTGCCTCAAAAAAAAAAAA+AGG + chr5_1:14198950-14198969 None:intergenic 20.0%
!!! AGTTTAATTTATCCTCAATA+TGG + chr5_1:14199026-14199045 None:intergenic 20.0%
!! TTGAGGATAAATTAAACTAT+AGG - chr5_1:14199028-14199047 Msa0719210:three_prime_UTR 20.0%
!! TATAATTTACCAAATAGTGT+TGG - chr5_1:14199748-14199767 Msa0719210:intron 20.0%
!! CAAGTATTATATGTATTGAA+TGG - chr5_1:14199966-14199985 Msa0719210:CDS 20.0%
!! TGATATATACATATTGCTAT+GGG + chr5_1:14200217-14200236 None:intergenic 20.0%
!! TTGATATATACATATTGCTA+TGG + chr5_1:14200218-14200237 None:intergenic 20.0%
!!! TTTAGAGGTATTTATTAGAA+TGG + chr5_1:14200293-14200312 None:intergenic 20.0%
!!! TAAAATTTCTAGAAATGGTA+AGG - chr5_1:14200401-14200420 Msa0719210:intron 20.0%
!!! AAAATTTCTAGAAATGGTAA+GGG - chr5_1:14200402-14200421 Msa0719210:intron 20.0%
!!! TCTAAAATACCTCAAAAAAA+GGG + chr5_1:14200440-14200459 None:intergenic 20.0%
!!! ATCTAAAATACCTCAAAAAA+AGG + chr5_1:14200441-14200460 None:intergenic 20.0%
!! AATATCTATGATTTCATATG+TGG + chr5_1:14200478-14200497 None:intergenic 20.0%
!!! TTGTTTTATATTTACTGTAG+TGG - chr5_1:14200619-14200638 Msa0719210:intron 20.0%
!! ACAATAAGATATATGATATG+AGG - chr5_1:14201017-14201036 Msa0719210:intron 20.0%
!!! CATAATTTTATTTTGGGTAA+AGG + chr5_1:14201276-14201295 None:intergenic 20.0%
!!! GGTGTACATAATTTTATTTT+GGG + chr5_1:14201282-14201301 None:intergenic 20.0%
!!! TGGTGTACATAATTTTATTT+TGG + chr5_1:14201283-14201302 None:intergenic 20.0%
!!! TTCTATCATATTTTCTATGA+AGG - chr5_1:14201453-14201472 Msa0719210:CDS 20.0%
!!! TCTATCATATTTTCTATGAA+GGG - chr5_1:14201454-14201473 Msa0719210:CDS 20.0%
! ACAAGAGAGAAAAACATTAA+TGG - chr5_1:14199053-14199072 Msa0719210:three_prime_UTR 25.0%
! CAAGAGAGAAAAACATTAAT+GGG - chr5_1:14199054-14199073 Msa0719210:three_prime_UTR 25.0%
! TTTCTCTAATCATCAAAGTT+TGG - chr5_1:14199257-14199276 Msa0719210:intron 25.0%
! GAAAAAGTTTGAAGATCTAA+AGG + chr5_1:14199500-14199519 None:intergenic 25.0%
! ATGAAGTAAGTTGTTTGTTT+AGG - chr5_1:14199833-14199852 Msa0719210:CDS 25.0%
! TGATTCAATCATTTCATCAA+GGG + chr5_1:14199914-14199933 None:intergenic 25.0%
! TTGATTCAATCATTTCATCA+AGG + chr5_1:14199915-14199934 None:intergenic 25.0%
! ACATATAATACTTGAACTTG+CGG + chr5_1:14199960-14199979 None:intergenic 25.0%
!! AAAAAAACTGATTTCTCTCA+TGG - chr5_1:14200551-14200570 Msa0719210:intron 25.0%
!! AAAAAACTGATTTCTCTCAT+GGG - chr5_1:14200552-14200571 Msa0719210:intron 25.0%
! TTGATGAGAAAATGACTATA+AGG - chr5_1:14200762-14200781 Msa0719210:intron 25.0%
! TGAACAAGACAATATATCTA+AGG - chr5_1:14201124-14201143 Msa0719210:intron 25.0%
!!! AATATTTATTGACTCTTGAC+TGG - chr5_1:14201383-14201402 Msa0719210:CDS 25.0%
CTTCTTTCATTCAAGAAACT+AGG - chr5_1:14199213-14199232 Msa0719210:CDS 30.0%
GATTCAAACTTCAATGAAGA+AGG + chr5_1:14199472-14199491 None:intergenic 30.0%
TAGCACTACTCTTAAACTAA+AGG + chr5_1:14199526-14199545 None:intergenic 30.0%
TCAACATATCCAACACTATT+TGG + chr5_1:14199760-14199779 None:intergenic 30.0%
TAATTCATTAACTAGTACCC+TGG - chr5_1:14200173-14200192 Msa0719210:intron 30.0%
! TTTTAGCAAATAGTTGAGCA+AGG - chr5_1:14200239-14200258 Msa0719210:intron 30.0%
ATGATTTCATATGTGGTCAA+AGG + chr5_1:14200471-14200490 None:intergenic 30.0%
TTTATCGAAAGCTACTATAG+TGG - chr5_1:14200590-14200609 Msa0719210:intron 30.0%
GACTATAAGGAAAACAATTG+AGG - chr5_1:14200775-14200794 Msa0719210:intron 30.0%
!!! GCAAATTTTGAAATACGTGA+TGG - chr5_1:14201483-14201502 Msa0719210:CDS 30.0%
GACACATGATATCCATATTG+AGG - chr5_1:14199011-14199030 Msa0719210:three_prime_UTR 35.0%
AACATTAATGGGAAAGAGCA+AGG - chr5_1:14199065-14199084 Msa0719210:three_prime_UTR 35.0%
!!! AAAGAGCAAGGTTTTAGTTG+TGG - chr5_1:14199077-14199096 Msa0719210:three_prime_UTR 35.0%
!!! AAGAGCAAGGTTTTAGTTGT+GGG - chr5_1:14199078-14199097 Msa0719210:three_prime_UTR 35.0%
TATTGGTAGGAGAATAGTGA+AGG - chr5_1:14199113-14199132 Msa0719210:CDS 35.0%
TACTTCAACGTCCAGATATA+GGG - chr5_1:14199166-14199185 Msa0719210:CDS 35.0%
GATGCAACTCAAACTTATTG+AGG - chr5_1:14199353-14199372 Msa0719210:intron 35.0%
CTTCATTGAAGTTTGAATCG+TGG - chr5_1:14199473-14199492 Msa0719210:CDS 35.0%
!! GCTAATGGTAATTTGTTGTG+TGG + chr5_1:14199888-14199907 None:intergenic 35.0%
AACATGTGTGGGTGTTTATT+GGG - chr5_1:14200267-14200286 Msa0719210:intron 35.0%
TAGAAATGGTAAGGGTTCAA+AGG - chr5_1:14200410-14200429 Msa0719210:intron 35.0%
TTTCATATGTGGTCAAAGGT+GGG + chr5_1:14200467-14200486 None:intergenic 35.0%
ATTTCATATGTGGTCAAAGG+TGG + chr5_1:14200468-14200487 None:intergenic 35.0%
GATGTAAGTGAAAGGAATGT+TGG + chr5_1:14200804-14200823 None:intergenic 35.0%
! TTTTGCTAATCATGTGAGCT+AGG - chr5_1:14200968-14200987 Msa0719210:intron 35.0%
TTTGCTAATCATGTGAGCTA+GGG - chr5_1:14200969-14200988 Msa0719210:intron 35.0%
!!! GTTTGTGGCAGTTGTTTTTA+TGG - chr5_1:14201047-14201066 Msa0719210:intron 35.0%
! TTGTCTTGTTCAATGTTCGA+GGG + chr5_1:14201116-14201135 None:intergenic 35.0%
! ATTGTCTTGTTCAATGTTCG+AGG + chr5_1:14201117-14201136 None:intergenic 35.0%
AAAGAGAGCTAGTTGAGAAA+TGG - chr5_1:14201169-14201188 Msa0719210:intron 35.0%
AAGAGAGCTAGTTGAGAAAT+GGG - chr5_1:14201170-14201189 Msa0719210:intron 35.0%
GAGAAATGGGAGAAACTTAT+TGG - chr5_1:14201183-14201202 Msa0719210:intron 35.0%
!! AAGAAAGTCTTGTTCGGATA+TGG + chr5_1:14201229-14201248 None:intergenic 35.0%
!! GGAAGAAAGAAAGTCTTGTT+CGG + chr5_1:14201235-14201254 None:intergenic 35.0%
GACTTTCTTTCTTCCATGAA+AGG - chr5_1:14201240-14201259 Msa0719210:intron 35.0%
TCTTGACTGGTGTGAAATTT+AGG - chr5_1:14201396-14201415 Msa0719210:CDS 35.0%
! ACTGGTGTGAAATTTAGGTT+TGG - chr5_1:14201401-14201420 Msa0719210:CDS 35.0%
GGATATCATGTGTCAAGCTA+AGG + chr5_1:14199005-14199024 None:intergenic 40.0%
!!! AGAGCAAGGTTTTAGTTGTG+GGG - chr5_1:14199079-14199098 Msa0719210:three_prime_UTR 40.0%
! GTGAAGGCAAGTTTAGAACA+AGG - chr5_1:14199129-14199148 Msa0719210:CDS 40.0%
ATCTGGACGTTGAAGTACAT+AGG + chr5_1:14199163-14199182 None:intergenic 40.0%
GTACTTCAACGTCCAGATAT+AGG - chr5_1:14199165-14199184 Msa0719210:CDS 40.0%
TCAGTTTCAAGCCCTATATC+TGG + chr5_1:14199180-14199199 None:intergenic 40.0%
!! GTTGTTATTTGCACCATGTC+TGG - chr5_1:14199303-14199322 Msa0719210:intron 40.0%
TCGAAAATGAACTCCAGACA+TGG + chr5_1:14199319-14199338 None:intergenic 40.0%
CAAACTTATTGAGGCCATCA+AGG - chr5_1:14199362-14199381 Msa0719210:intron 40.0%
!! TCTTTTGTGTGTCGATGCTT+CGG - chr5_1:14199635-14199654 Msa0719210:intron 40.0%
TTTCATCAAGGGACAGCTAA+TGG + chr5_1:14199903-14199922 None:intergenic 40.0%
GTATAACTTGACTAGTACCC+CGG - chr5_1:14200036-14200055 Msa0719210:intron 40.0%
TTAGCTGCTTAACTAGTGTC+CGG + chr5_1:14200058-14200077 None:intergenic 40.0%
TTCATTAACTAGTACCCTGG+AGG - chr5_1:14200176-14200195 Msa0719210:intron 40.0%
AGCAAGGTAAAGAACATGTG+TGG - chr5_1:14200255-14200274 Msa0719210:intron 40.0%
GCAAGGTAAAGAACATGTGT+GGG - chr5_1:14200256-14200275 Msa0719210:intron 40.0%
GAACATGTGTGGGTGTTTAT+TGG - chr5_1:14200266-14200285 Msa0719210:intron 40.0%
AAATGGTAAGGGTTCAAAGG+TGG - chr5_1:14200413-14200432 Msa0719210:intron 40.0%
!!! AAAGGTGGACCCTTTTTTTG+AGG - chr5_1:14200428-14200447 Msa0719210:intron 40.0%
CGTTTCCTACCTTCAGAATT+TGG - chr5_1:14200689-14200708 Msa0719210:intron 40.0%
! CCATTCCAAATTCTGAAGGT+AGG + chr5_1:14200697-14200716 None:intergenic 40.0%
CCTACCTTCAGAATTTGGAA+TGG - chr5_1:14200694-14200713 Msa0719210:intron 40.0%
TCATCAAATGTGACTCTTCC+TGG + chr5_1:14200749-14200768 None:intergenic 40.0%
! CTTTGAGTGAGGATGTTTTC+TGG + chr5_1:14201153-14201172 None:intergenic 40.0%
! AGGGTGTGTTTTACCTTTCA+TGG + chr5_1:14201256-14201275 None:intergenic 40.0%
! TGAAATACGTGATGGTGAAG+AGG - chr5_1:14201491-14201510 Msa0719210:CDS 40.0%
!!! GAGCAAGGTTTTAGTTGTGG+GGG - chr5_1:14199080-14199099 Msa0719210:three_prime_UTR 45.0%
!!! AGCAAGGTTTTAGTTGTGGG+GGG - chr5_1:14199081-14199100 Msa0719210:three_prime_UTR 45.0%
!!! TTTTAGTTGTGGGGGGTACT+GGG - chr5_1:14199088-14199107 Msa0719210:exon 45.0%
GAAACTAGGTGCTCATCTTG+TGG - chr5_1:14199227-14199246 Msa0719210:CDS 45.0%
TAGCTGCTTAACTAGTGTCC+GGG + chr5_1:14200057-14200076 None:intergenic 45.0%
GGGTCCATTCCAAATTCTGA+AGG + chr5_1:14200701-14200720 None:intergenic 45.0%
TTGGCGGAGATGTAAGTGAA+AGG + chr5_1:14200812-14200831 None:intergenic 45.0%
TCCCATTTGAGAGAGGTTAC+CGG + chr5_1:14200855-14200874 None:intergenic 45.0%
! AGAGTGTTCCCATTTGAGAG+AGG + chr5_1:14200862-14200881 None:intergenic 45.0%
TAGAGAACAACCTTGTCTCG+TGG + chr5_1:14200890-14200909 None:intergenic 45.0%
CGAGACAAGGTTGTTCTCTA+TGG - chr5_1:14200890-14200909 Msa0719210:intron 45.0%
AAGGTTGTTCTCTATGGCGA+CGG - chr5_1:14200896-14200915 Msa0719210:intron 45.0%
ATATGAGGTGCGCATGTTTG+TGG - chr5_1:14201032-14201051 Msa0719210:intron 45.0%
! TGAGTGAGGATGTTTTCTGG+TGG + chr5_1:14201150-14201169 None:intergenic 45.0%
! CAACTAGCTCTCTTTGAGTG+AGG + chr5_1:14201164-14201183 None:intergenic 45.0%
! GGCATCAAAGCTTTATCCAG+AGG - chr5_1:14201512-14201531 Msa0719210:CDS 45.0%
!!! TTATTTTACTAAAAATATTA+AGG + chr5_1:14200336-14200355 None:intergenic 5.0%
!! CAATTTATTAAAAAAAAAAA+AGG - chr5_1:14200365-14200384 Msa0719210:intron 5.0%
!! AATAATAAAAAATATACATT+GGG - chr5_1:14201646-14201665 Msa0719210:five_prime_UTR 5.0%
!!! GTTTTAGTTGTGGGGGGTAC+TGG - chr5_1:14199087-14199106 Msa0719210:exon 50.0%
!! GGGGTACTGGGTATATTGGT+AGG - chr5_1:14199100-14199119 Msa0719210:exon 50.0%
CTAGGTGCTCATCTTGTGGA+AGG - chr5_1:14199231-14199250 Msa0719210:CDS 50.0%
ATTGAGGCCATCAAGGATGC+AGG - chr5_1:14199369-14199388 Msa0719210:intron 50.0%
TTGAGGCCATCAAGGATGCA+GGG - chr5_1:14199370-14199389 Msa0719210:intron 50.0%
GCTTGAGCTAGCTAGGTACT+AGG + chr5_1:14199938-14199957 None:intergenic 50.0%
AGCTGCTTAACTAGTGTCCG+GGG + chr5_1:14200056-14200075 None:intergenic 50.0%
TAACTAGTACCCTGGAGGCA+CGG - chr5_1:14200181-14200200 Msa0719210:intron 50.0%
AATGTTAACCCGTGCCTCCA+GGG + chr5_1:14200193-14200212 None:intergenic 50.0%
AAATGTTAACCCGTGCCTCC+AGG + chr5_1:14200194-14200213 None:intergenic 50.0%
CATATGTGGTCAAAGGTGGG+TGG + chr5_1:14200464-14200483 None:intergenic 50.0%
GGTAGGAAACGCTAGTGGAA+AGG + chr5_1:14200680-14200699 None:intergenic 50.0%
CTGAAGGTAGGAAACGCTAG+TGG + chr5_1:14200685-14200704 None:intergenic 50.0%
TGGAATGGACCCAGCTCTTA+TGG - chr5_1:14200709-14200728 Msa0719210:intron 50.0%
GGAATGGACCCAGCTCTTAT+GGG - chr5_1:14200710-14200729 Msa0719210:intron 50.0%
GTGCATGTCCCATAAGAGCT+GGG + chr5_1:14200721-14200740 None:intergenic 50.0%
AGTGCATGTCCCATAAGAGC+TGG + chr5_1:14200722-14200741 None:intergenic 50.0%
ATGGGACATGCACTTGAACC+AGG - chr5_1:14200728-14200747 Msa0719210:intron 50.0%
AAAGTAGGCCGCGAAACAGT+TGG + chr5_1:14200831-14200850 None:intergenic 50.0%
AGAGAGGTTACCGGCAAAGT+AGG + chr5_1:14200846-14200865 None:intergenic 50.0%
TGCCGGTAACCTCTCTCAAA+TGG - chr5_1:14200850-14200869 Msa0719210:intron 50.0%
GCCGGTAACCTCTCTCAAAT+GGG - chr5_1:14200851-14200870 Msa0719210:intron 50.0%
ACTCTTTCCTCCACGAGACA+AGG - chr5_1:14200877-14200896 Msa0719210:intron 50.0%
AGAACAACCTTGTCTCGTGG+AGG + chr5_1:14200887-14200906 None:intergenic 50.0%
TATGGCGACGGCAATGTCAA+AGG - chr5_1:14200908-14200927 Msa0719210:intron 50.0%
TTGGACCTTGCAAGCCAAGT+AGG - chr5_1:14201420-14201439 Msa0719210:CDS 50.0%
CTTGCAAGCCAAGTAGGAGT+GGG - chr5_1:14201426-14201445 Msa0719210:CDS 50.0%
TTGCAAGCCAAGTAGGAGTG+GGG - chr5_1:14201427-14201446 Msa0719210:CDS 50.0%
AGAAATGCCCCACTCCTACT+TGG + chr5_1:14201437-14201456 None:intergenic 50.0%
ATGCGTGTGTATTGCACCTC+TGG + chr5_1:14201531-14201550 None:intergenic 50.0%
! AGAGGTGCAATACACACGCA+TGG - chr5_1:14201530-14201549 Msa0719210:CDS 50.0%
!! GTGGGGGGTACTGGGTATAT+TGG - chr5_1:14199096-14199115 Msa0719210:exon 55.0%
GACGTTCCCTGCATCCTTGA+TGG + chr5_1:14199379-14199398 None:intergenic 55.0%
CAAGGATGCAGGGAACGTCA+AGG - chr5_1:14199380-14199399 Msa0719210:intron 55.0%
ACTTGCGGCTTGAGCTAGCT+AGG + chr5_1:14199945-14199964 None:intergenic 55.0%
! AACTAGTACCCTGGAGGCAC+GGG - chr5_1:14200182-14200201 Msa0719210:intron 55.0%
CATCTCCGCCAACTGTTTCG+CGG - chr5_1:14200820-14200839 Msa0719210:intron 55.0%
TGTTTCGCGGCCTACTTTGC+CGG - chr5_1:14200833-14200852 Msa0719210:intron 55.0%
!! CCACTCCTACTTGGCTTGCA+AGG + chr5_1:14201428-14201447 None:intergenic 55.0%
CCTTGCAAGCCAAGTAGGAG+TGG - chr5_1:14201425-14201444 Msa0719210:CDS 55.0%
GTAGGCCGCGAAACAGTTGG+CGG + chr5_1:14200828-14200847 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 14198924 14201757 14198924 ID=Msa0719210;Name=Msa0719210
chr5_1 mRNA 14198924 14201757 14198924 ID=Msa0719210-mRNA-1;Parent=Msa0719210;Name=Msa0719210-mRNA-1;_AED=0.07;_eAED=0.07;_QI=148|1|1|1|1|1|4|179|311
chr5_1 exon 14198924 14199263 14198924 ID=Msa0719210-mRNA-1:exon:5620;Parent=Msa0719210-mRNA-1
chr5_1 exon 14199420 14199623 14199420 ID=Msa0719210-mRNA-1:exon:5619;Parent=Msa0719210-mRNA-1
chr5_1 exon 14199752 14199992 14199752 ID=Msa0719210-mRNA-1:exon:5618;Parent=Msa0719210-mRNA-1
chr5_1 exon 14201280 14201757 14201280 ID=Msa0719210-mRNA-1:exon:5617;Parent=Msa0719210-mRNA-1
chr5_1 five_prime_UTR 14201610 14201757 14201610 ID=Msa0719210-mRNA-1:five_prime_utr;Parent=Msa0719210-mRNA-1
chr5_1 CDS 14201280 14201609 14201280 ID=Msa0719210-mRNA-1:cds;Parent=Msa0719210-mRNA-1
chr5_1 CDS 14199752 14199992 14199752 ID=Msa0719210-mRNA-1:cds;Parent=Msa0719210-mRNA-1
chr5_1 CDS 14199420 14199623 14199420 ID=Msa0719210-mRNA-1:cds;Parent=Msa0719210-mRNA-1
chr5_1 CDS 14199103 14199263 14199103 ID=Msa0719210-mRNA-1:cds;Parent=Msa0719210-mRNA-1
chr5_1 three_prime_UTR 14198924 14199102 14198924 ID=Msa0719210-mRNA-1:three_prime_utr;Parent=Msa0719210-mRNA-1
Gene Sequence

>Msa0719210

ATGGGAAAGAGCAAGGTTTTAGTTGTGGGGGGTACTGGGTATATTGGTAGGAGAATAGTGAAGGCAAGTTTAGAACAAGGTCATGAAACCTATGTACTTCAACGTCCAGATATAGGGCTTGAAACTGAGAAAGTGCAGATGCTTCTTTCATTCAAGAAACTAGGTGCTCATCTTGTGGAAGGTTCTTTCTCTAATCATCAAAGTTTGGTAGATGCTGTGAAACTAGTTGATGTTGTTATTTGCACCATGTCTGGAGTTCATTTTCGATCTCACAACTTGATGATGCAACTCAAACTTATTGAGGCCATCAAGGATGCAGGGAACGTCAAGCGTTTCCTACCTTCAGAATTTGGAATGGACCCAGCTCTTATGGGACATGCACTTGAACCAGGAAGAGTCACATTTGATGAGAAAATGACTATAAGGAAAACAATTGAGGATGCCAACATTCCTTTCACTTACATCTCCGCCAACTGTTTCGCGGCCTACTTTGCCGGTAACCTCTCTCAAATGGGAACACTCTTTCCTCCACGAGACAAGGTTGTTCTCTATGGCGACGGCAATGTCAAAGTTGTTTTTATGGATGAAGATGATGTTGCAACATACACAATTAAGACAATTAATGACCCTCGAACATTGAACAAGACAATATATCTAAGGCCACCAGAAAACATCCTCACTCAAAGAGAGCTAGTTGAGAAATGGGAGAAACTTATTGGAAAGCAACTAGAGAAGTCTACCATATCCGAACAAGACTTTCTTTCTTCCATGAAAGGTTTGGACCTTGCAAGCCAAGTAGGAGTGGGGCATTTCTATCATATTTTCTATGAAGGGTGTTTAGCAAATTTTGAAATACGTGATGGTGAAGAGGCATCAAAGCTTTATCCAGAGGTGCAATACACACGCATGGATGAATATCTAAAATTATATGCGTGA
Protein sequence

>Msa0719210

MGKSKVLVVGGTGYIGRRIVKASLEQGHETYVLQRPDIGLETEKVQMLLSFKKLGAHLVEGSFSNHQSLVDAVKLVDVVICTMSGVHFRSHNLMMQLKLIEAIKDAGNVKRFLPSEFGMDPALMGHALEPGRVTFDEKMTIRKTIEDANIPFTYISANCFAAYFAGNLSQMGTLFPPRDKVVLYGDGNVKVVFMDEDDVATYTIKTINDPRTLNKTIYLRPPENILTQRELVEKWEKLIGKQLEKSTISEQDFLSSMKGLDLASQVGVGHFYHIFYEGCLANFEIRDGEEASKLYPEVQYTRMDEYLKLYA*