AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0736130

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0736130 MtrunA17_Chr5g0433481 94.000 150 9 0 1 150 1 150 6.97e-103 291
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0736130 AT1G65820.1 66.667 144 48 0 1 144 1 144 1.46e-67 201
Msa0736130 AT1G65820.2 60.000 160 48 1 1 144 1 160 2.98e-63 191
Msa0736130 AT1G65820.3 70.492 122 36 0 1 122 1 122 1.52e-60 186

Find 74 sgRNAs with CRISPR-Local

Find 285 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCTTCATGTTGATGATTT+TGG 0.072271 5_1:-52082119 Msa0736130:CDS
TTGGCCAAATATGTTGTTTC+TGG 0.148618 5_1:+52081068 None:intergenic
AATCATCAACATGAAGAAAA+TGG 0.174934 5_1:+52082123 None:intergenic
ATACGGTTTGGATACGTTTA+TGG 0.180137 5_1:+52081207 None:intergenic
TTACCCAGAAACAACATATT+TGG 0.195988 5_1:-52081072 Msa0736130:three_prime_UTR
CGCAGCATATATCCCAAGTA+TGG 0.286186 5_1:+52081178 None:intergenic
GCACAAACCAAACCTGGATT+TGG 0.292617 5_1:+52081105 None:intergenic
CCCGAGTTTCAGACGGTTCT+CGG 0.306867 5_1:+52082003 None:intergenic
AGTTCAAATCACTGGTTTGC+AGG 0.309105 5_1:+52081768 None:intergenic
CATTATTCATTTGCAGTCCT+TGG 0.316092 5_1:+52081692 None:intergenic
AGCCTTTGTATGTTACAATA+AGG 0.341454 5_1:-52081634 Msa0736130:three_prime_UTR
TTTCAGATTCAGAAGCATAA+AGG 0.349675 5_1:+52083989 None:intergenic
ACCGTATTTGCACCCATACT+TGG 0.354211 5_1:-52081191 Msa0736130:three_prime_UTR
GAAATGTTACCAAAGGAATA+TGG 0.359770 5_1:-52084202 Msa0736130:CDS
CAGATGTCTCATTGCGGACT+TGG 0.362574 5_1:-52081433 Msa0736130:three_prime_UTR
GCATCGTTTCCAGAGAGTTT+TGG 0.363401 5_1:+52082146 None:intergenic
ATGGTGTCTACACTTTCATT+TGG 0.365189 5_1:-52081810 Msa0736130:CDS
CCTATCATTTGTGCTGTCCT+TGG 0.365216 5_1:-52082082 Msa0736130:CDS
TGCTAAAAGTATTTAAGTTA+TGG 0.373607 5_1:-52081404 Msa0736130:three_prime_UTR
GCCGGCACTACTGGGGCTTA+TGG 0.381706 5_1:-52081829 Msa0736130:CDS
CCCAAGTATGGGTGCAAATA+CGG 0.388717 5_1:+52081190 None:intergenic
TGGCCAAATATGTTGTTTCT+GGG 0.400903 5_1:+52081069 None:intergenic
CAAGCTCTTCAATTGCGTTC+AGG 0.403771 5_1:-52083956 Msa0736130:intron
TTCAGATTCAGAAGCATAAA+GGG 0.404409 5_1:+52083990 None:intergenic
GCCACAAAGCCATATTCCTT+TGG 0.406868 5_1:+52084193 None:intergenic
CACAAACCAAACCTGGATTT+GGG 0.408579 5_1:+52081106 None:intergenic
AAGGCTAAAACTCCAAAATC+TGG 0.416056 5_1:+52081290 None:intergenic
TTGGCCTTGTTTAACAGTAA+AGG 0.430630 5_1:-52081261 Msa0736130:three_prime_UTR
ATCATCAACATGAAGAAAAT+GGG 0.438900 5_1:+52082124 None:intergenic
CCAAGGACAGCACAAATGAT+AGG 0.451302 5_1:+52082082 None:intergenic
GAGATAAAAGTTCAAATCAC+TGG 0.454575 5_1:+52081760 None:intergenic
GTATCTCCCAAATCCAGGTT+TGG 0.456029 5_1:-52081112 Msa0736130:three_prime_UTR
AACTGTAATATCTAAAATAC+AGG 0.460383 5_1:+52081136 None:intergenic
CCGAGAACCGTCTGAAACTC+GGG 0.463273 5_1:-52082003 Msa0736130:intron
GAGAGGGCACCAAAACTCTC+TGG 0.465785 5_1:-52082155 Msa0736130:CDS
CCGAGTTTCAGACGGTTCTC+GGG 0.472239 5_1:+52082004 None:intergenic
TGTCTACACTTTCATTTGGA+TGG 0.477493 5_1:-52081806 Msa0736130:CDS
GTATGGGTGCAAATACGGTT+TGG 0.478873 5_1:+52081195 None:intergenic
CAACGAGATGAGTAATGTAC+TGG 0.483795 5_1:+52084283 None:intergenic
AGTTCGTATCTCCCAAATCC+AGG 0.485107 5_1:-52081117 Msa0736130:three_prime_UTR
CCCGAGAACCGTCTGAAACT+CGG 0.487007 5_1:-52082004 Msa0736130:CDS
CAAAATGCACAAACCAAACC+TGG 0.488178 5_1:+52081099 None:intergenic
GCGAGCTTTGCCAACTTGAA+AGG 0.489911 5_1:+52084129 None:intergenic
ACCAAAGGAATATGGCTTTG+TGG 0.496319 5_1:-52084194 Msa0736130:CDS
ACCATAAGCCCCAGTAGTGC+CGG 0.497715 5_1:+52081828 None:intergenic
GAAAGGTCATGAAGAAATTG+AGG 0.509106 5_1:+52084146 None:intergenic
GATTCAGAAGCATAAAGGGT+TGG 0.509643 5_1:+52083994 None:intergenic
TTACTTTGACCACTAGTACA+TGG 0.520685 5_1:+52081370 None:intergenic
TTCTTCATGACCTTTCAAGT+TGG 0.523551 5_1:-52084139 Msa0736130:CDS
ACCTCATCATCAAGAGGCTA+TGG 0.525062 5_1:+52081732 None:intergenic
CCGTATTTGCACCCATACTT+GGG 0.532717 5_1:-52081190 Msa0736130:three_prime_UTR
TTTAAGTTATGGTAGAACCT+TGG 0.539817 5_1:-52081393 Msa0736130:three_prime_UTR
CTGAACGCAATTGAAGAGCT+TGG 0.540036 5_1:+52083957 None:intergenic
TAAACAAGGCCAATTAAGAA+AGG 0.541354 5_1:+52081271 None:intergenic
TGAGTAATGTACTGGTTTCG+TGG 0.544472 5_1:+52084291 None:intergenic
AATTGACGACTACACTCTGC+AGG 0.557869 5_1:+52081486 None:intergenic
GAGTGTAGTCGTCAATTCAC+AGG 0.564595 5_1:-52081480 Msa0736130:three_prime_UTR
TTAATAACCTCATCATCAAG+AGG 0.568571 5_1:+52081726 None:intergenic
ACCATAGCCTCTTGATGATG+AGG 0.570853 5_1:-52081733 Msa0736130:three_prime_UTR
ACGGTTCTCGGGTTTGCCTG+TGG 0.592329 5_1:+52082015 None:intergenic
GGTCATGAAGAAATTGAGGA+AGG 0.595983 5_1:+52084150 None:intergenic
GCTAGATACTTCTACTTCGT+AGG 0.595990 5_1:-52082043 Msa0736130:CDS
GCAGCATATATCCCAAGTAT+GGG 0.604149 5_1:+52081179 None:intergenic
GAACCTTGGCCATGTACTAG+TGG 0.609927 5_1:-52081379 Msa0736130:three_prime_UTR
ACATGTTTAGAGTTTCAACA+TGG 0.610280 5_1:-52081611 Msa0736130:three_prime_UTR
GTCCTTATTGTAACATACAA+AGG 0.616409 5_1:+52081632 None:intergenic
TACTTCGTAGGCTATGCCAC+AGG 0.617511 5_1:-52082031 Msa0736130:CDS
TTAATCACCCGAGTTTCAGA+CGG 0.625019 5_1:+52081996 None:intergenic
ACTGATAGAAATGTTACCAA+AGG 0.627858 5_1:-52084209 Msa0736130:CDS
TGACCACTAGTACATGGCCA+AGG 0.640168 5_1:+52081376 None:intergenic
CAACAGTGTAAAGTACTCCA+AGG 0.641397 5_1:+52082065 None:intergenic
CATGTTTAGAGTTTCAACAT+GGG 0.654346 5_1:-52081610 Msa0736130:three_prime_UTR
TATTAAATAATATAAGACCA+AGG 0.701183 5_1:-52081709 Msa0736130:three_prime_UTR
CTAAATCAGATGTCTCATTG+CGG 0.727194 5_1:-52081439 Msa0736130:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATGCTAAAAATAATTAAT+TGG - chr5_1:52081697-52081716 Msa0736130:three_prime_UTR 10.0%
!!! ATGGTTTTTTTTTTTTTTTT+TGG + chr5_1:52083507-52083526 None:intergenic 10.0%
!! AAAGTAAAATAAATCATGTT+CGG + chr5_1:52081559-52081578 None:intergenic 15.0%
!! ATATTTGTAACATATCAATA+TGG + chr5_1:52081791-52081810 None:intergenic 15.0%
!! TACAAATATATATATGCTAA+AGG - chr5_1:52081802-52081821 Msa0736130:CDS 15.0%
!! ACAAATATATATATGCTAAA+GGG - chr5_1:52081803-52081822 Msa0736130:CDS 15.0%
!!! ATTATTTATACCTTTTAGAA+AGG + chr5_1:52082046-52082065 None:intergenic 15.0%
!! CAATAAATTGTTAAAAAGAT+AGG + chr5_1:52082648-52082667 None:intergenic 15.0%
!!! CTATCTTTTTAACAATTTAT+TGG - chr5_1:52082646-52082665 Msa0736130:intron 15.0%
!! TATTAAATAATATAAGACCA+AGG - chr5_1:52083696-52083715 Msa0736130:intron 15.0%
!! CATCTATATAAACAAATTGA+AGG + chr5_1:52081328-52081347 None:intergenic 20.0%
!!! ATTTTACAAAATAAACTGAC+TGG - chr5_1:52081602-52081621 Msa0736130:three_prime_UTR 20.0%
!! ATTTAGTATTAACAATTCCA+TGG - chr5_1:52081832-52081851 Msa0736130:CDS 20.0%
!! AATTATTTCGACTAATCAAT+TGG - chr5_1:52082020-52082039 Msa0736130:CDS 20.0%
!!! CTAAAAGGTATAAATAATGT+TGG - chr5_1:52082048-52082067 Msa0736130:CDS 20.0%
!! TAAAATCAAACAATCTTTAC+TGG + chr5_1:52082115-52082134 None:intergenic 20.0%
!!! TTTGTTATAGAGAAAATTCT+TGG + chr5_1:52082541-52082560 None:intergenic 20.0%
!! TAACAATTTATTGGAAGATT+TGG - chr5_1:52082655-52082674 Msa0736130:intron 20.0%
!!! TTATTTTTTTGAATCAGCAA+AGG + chr5_1:52083148-52083167 None:intergenic 20.0%
!! TTGTTATAATATTCTGTGTT+TGG - chr5_1:52083219-52083238 Msa0736130:intron 20.0%
!! AATTACAGATATAACACTTA+TGG + chr5_1:52083448-52083467 None:intergenic 20.0%
!! TAATTAAAACCATGATTGAT+TGG - chr5_1:52083463-52083482 Msa0736130:intron 20.0%
!! AATTAAAACCATGATTGATT+GGG - chr5_1:52083464-52083483 Msa0736130:intron 20.0%
!! AGTTACATATTAATCAATCA+TGG + chr5_1:52083526-52083545 None:intergenic 20.0%
!!! TGCTAAAAGTATTTAAGTTA+TGG - chr5_1:52084001-52084020 Msa0736130:CDS 20.0%
!! AAATATAAAACCAAAAACAG+GGG + chr5_1:52084094-52084113 None:intergenic 20.0%
!! CAAATATAAAACCAAAAACA+GGG + chr5_1:52084095-52084114 None:intergenic 20.0%
!!! AACTGTAATATCTAAAATAC+AGG + chr5_1:52084272-52084291 None:intergenic 20.0%
!!! AAGATGAGTTTGTTTTTTTC+TGG + chr5_1:52081162-52081181 None:intergenic 25.0%
!!! AGTTTGTGATTTTGTTTACA+AGG - chr5_1:52081350-52081369 Msa0736130:three_prime_UTR 25.0%
! AAGTTACGAATCTTATTGTT+TGG + chr5_1:52081532-52081551 None:intergenic 25.0%
! GGATAAATGTTGAATAATCT+AGG - chr5_1:52081623-52081642 Msa0736130:three_prime_UTR 25.0%
! AAATGTTGAATAATCTAGGA+TGG - chr5_1:52081627-52081646 Msa0736130:three_prime_UTR 25.0%
! TATAAATAATGTTGGTCGAT+GGG - chr5_1:52082056-52082075 Msa0736130:CDS 25.0%
!!! TTTAAAAGTCGATTTTGAGA+AGG - chr5_1:52082131-52082150 Msa0736130:CDS 25.0%
!! AATTTATTGGAAGATTTGGT+AGG - chr5_1:52082659-52082678 Msa0736130:intron 25.0%
! AAAATCTATGTATGTGAAGT+TGG - chr5_1:52082748-52082767 Msa0736130:intron 25.0%
! GATAGACAAAAAAAGAATCT+AGG + chr5_1:52083188-52083207 None:intergenic 25.0%
! ATCATCAACATGAAGAAAAT+GGG + chr5_1:52083284-52083303 None:intergenic 25.0%
! AATCATCAACATGAAGAAAA+TGG + chr5_1:52083285-52083304 None:intergenic 25.0%
!!! TTTCTTCATGTTGATGATTT+TGG - chr5_1:52083286-52083305 Msa0736130:intron 25.0%
!!! TTCTTCATGTTGATGATTTT+GGG - chr5_1:52083287-52083306 Msa0736130:intron 25.0%
!!! TTTTTTTTTGGTACAAGAGA+GGG + chr5_1:52083495-52083514 None:intergenic 25.0%
!!! TTTTTTTTTTGGTACAAGAG+AGG + chr5_1:52083496-52083515 None:intergenic 25.0%
! AACTGAATTTATATGCTAAC+AGG - chr5_1:52083542-52083561 Msa0736130:intron 25.0%
! ACTGAATTTATATGCTAACA+GGG - chr5_1:52083543-52083562 Msa0736130:intron 25.0%
! GCAAATATAAAACCAAAAAC+AGG + chr5_1:52084096-52084115 None:intergenic 25.0%
!! GTTTTATATTTGCCAGATTT+TGG - chr5_1:52084103-52084122 Msa0736130:intron 25.0%
!! TTTTCCTTTACTGTTAAACA+AGG + chr5_1:52084151-52084170 None:intergenic 25.0%
ACAAACTCATCTTCCAAAAA+TGG - chr5_1:52081169-52081188 Msa0736130:three_prime_UTR 30.0%
ACTGATAGAAATGTTACCAA+AGG - chr5_1:52081196-52081215 Msa0736130:three_prime_UTR 30.0%
GAAATGTTACCAAAGGAATA+TGG - chr5_1:52081203-52081222 Msa0736130:three_prime_UTR 30.0%
TAAAAAACTTTGTACCTGTG+AGG + chr5_1:52081390-52081409 None:intergenic 30.0%
! TTCAGATTCAGAAGCATAAA+GGG + chr5_1:52081418-52081437 None:intergenic 30.0%
! TTTCAGATTCAGAAGCATAA+AGG + chr5_1:52081419-52081438 None:intergenic 30.0%
ACGAATCTTATTGTTTGGTT+TGG + chr5_1:52081527-52081546 None:intergenic 30.0%
!! GAATAATCTAGGATGGATTT+TGG - chr5_1:52081634-52081653 Msa0736130:three_prime_UTR 30.0%
ATACTACCTAACAAGAAAAG+AGG + chr5_1:52081946-52081965 None:intergenic 30.0%
!! AATCAATTGGCCTTTCTAAA+AGG - chr5_1:52082033-52082052 Msa0736130:CDS 30.0%
GTATAAATAATGTTGGTCGA+TGG - chr5_1:52082055-52082074 Msa0736130:CDS 30.0%
ATAAATAATGTTGGTCGATG+GGG - chr5_1:52082057-52082076 Msa0736130:CDS 30.0%
!! GAGGTTTTTGGATATATCAA+TGG - chr5_1:52082173-52082192 Msa0736130:intron 30.0%
GGAAATTAATTTCTTCCGTT+GGG + chr5_1:52082271-52082290 None:intergenic 30.0%
TGGAAATTAATTTCTTCCGT+TGG + chr5_1:52082272-52082291 None:intergenic 30.0%
ACGGAAGAAATTAATTTCCA+AGG - chr5_1:52082272-52082291 Msa0736130:intron 30.0%
! TCAAAATTGGTTTAACACAG+AGG - chr5_1:52082432-52082451 Msa0736130:intron 30.0%
AAAGGAAGAAACAAGTACAA+AGG + chr5_1:52083081-52083100 None:intergenic 30.0%
! CTTCCTTTATGCAAACTTTT+TGG - chr5_1:52083093-52083112 Msa0736130:intron 30.0%
CTGAATTTATATGCTAACAG+GGG - chr5_1:52083544-52083563 Msa0736130:intron 30.0%
GAGATAAAAGTTCAAATCAC+TGG + chr5_1:52083648-52083667 None:intergenic 30.0%
TTAATAACCTCATCATCAAG+AGG + chr5_1:52083682-52083701 None:intergenic 30.0%
AGCCTTTGTATGTTACAATA+AGG - chr5_1:52083771-52083790 Msa0736130:intron 30.0%
GTCCTTATTGTAACATACAA+AGG + chr5_1:52083776-52083795 None:intergenic 30.0%
! ACATGTTTAGAGTTTCAACA+TGG - chr5_1:52083794-52083813 Msa0736130:intron 30.0%
! CATGTTTAGAGTTTCAACAT+GGG - chr5_1:52083795-52083814 Msa0736130:intron 30.0%
! TTTAAGTTATGGTAGAACCT+TGG - chr5_1:52084012-52084031 Msa0736130:CDS 30.0%
!! GAGTTTTAGCCTTTCTTAAT+TGG - chr5_1:52084125-52084144 Msa0736130:CDS 30.0%
TAAACAAGGCCAATTAAGAA+AGG + chr5_1:52084137-52084156 None:intergenic 30.0%
TTACCCAGAAACAACATATT+TGG - chr5_1:52084333-52084352 Msa0736130:five_prime_UTR 30.0%
GAAAGGTCATGAAGAAATTG+AGG + chr5_1:52081262-52081281 None:intergenic 35.0%
! TTCTTCATGACCTTTCAAGT+TGG - chr5_1:52081266-52081285 Msa0736130:three_prime_UTR 35.0%
! TGTTTGTTGAAATTCCTCAC+AGG - chr5_1:52081373-52081392 Msa0736130:three_prime_UTR 35.0%
TGATTCCTATGTTCCTTGTT+GGG - chr5_1:52081736-52081755 Msa0736130:three_prime_UTR 35.0%
TTAACAATTCCATGGCAATG+AGG - chr5_1:52081840-52081859 Msa0736130:CDS 35.0%
CGATAATCTTTCAACTCCAA+AGG + chr5_1:52081922-52081941 None:intergenic 35.0%
! TATCGTCCTCTTTTCTTGTT+AGG - chr5_1:52081937-52081956 Msa0736130:intron 35.0%
!! TTTTGAGAAGGCTTTTGACT+CGG - chr5_1:52082143-52082162 Msa0736130:CDS 35.0%
GATATATCAATGGTGAGAGT+TGG - chr5_1:52082183-52082202 Msa0736130:intron 35.0%
ATGTCGATACTTGTGAATGA+TGG - chr5_1:52082245-52082264 Msa0736130:intron 35.0%
! AAGGATTTTGTGAACCTAGA+GGG - chr5_1:52082400-52082419 Msa0736130:intron 35.0%
AAATAACCAAGACAGAGATG+TGG - chr5_1:52082558-52082577 Msa0736130:intron 35.0%
ATGTGGATAGATGGTTCAAA+TGG - chr5_1:52082575-52082594 Msa0736130:intron 35.0%
TGTGGATAGATGGTTCAAAT+GGG - chr5_1:52082576-52082595 Msa0736130:intron 35.0%
! AAGATTTGGTAGGCTATACA+AGG - chr5_1:52082669-52082688 Msa0736130:intron 35.0%
! CAAGGCATCAAGAATATGAT+AGG - chr5_1:52082687-52082706 Msa0736130:intron 35.0%
! AAGAATATGATAGGTGGACT+TGG - chr5_1:52082696-52082715 Msa0736130:intron 35.0%
TCTATGTATGTGAAGTTGGA+AGG - chr5_1:52082752-52082771 Msa0736130:intron 35.0%
AGAGGATGTTTCGTCAAATT+TGG - chr5_1:52082813-52082832 Msa0736130:intron 35.0%
TTCGTCAAATTTGGAAGTCT+AGG - chr5_1:52082822-52082841 Msa0736130:intron 35.0%
TCGTCAAATTTGGAAGTCTA+GGG - chr5_1:52082823-52082842 Msa0736130:intron 35.0%
TATCATACCAGTATTCGCAT+TGG + chr5_1:52082890-52082909 None:intergenic 35.0%
!! ACAAAGGTGCTGTAAATATG+TGG + chr5_1:52083065-52083084 None:intergenic 35.0%
GCACCAAAAAGTTTGCATAA+AGG + chr5_1:52083099-52083118 None:intergenic 35.0%
!!! TTCATGTTGATGATTTTGGG+AGG - chr5_1:52083290-52083309 Msa0736130:intron 35.0%
!!! TCATGTTGATGATTTTGGGA+GGG - chr5_1:52083291-52083310 Msa0736130:intron 35.0%
!!! TAACAGGGGATTTTTTATGC+CGG - chr5_1:52083558-52083577 Msa0736130:intron 35.0%
ATGGTGTCTACACTTTCATT+TGG - chr5_1:52083595-52083614 Msa0736130:intron 35.0%
TGTCTACACTTTCATTTGGA+TGG - chr5_1:52083599-52083618 Msa0736130:intron 35.0%
CATTATTCATTTGCAGTCCT+TGG + chr5_1:52083716-52083735 None:intergenic 35.0%
CTAAATCAGATGTCTCATTG+CGG - chr5_1:52083966-52083985 Msa0736130:CDS 35.0%
TTACTTTGACCACTAGTACA+TGG + chr5_1:52084038-52084057 None:intergenic 35.0%
! TACACTATTTCCCCTGTTTT+TGG - chr5_1:52084081-52084100 Msa0736130:intron 35.0%
AAGGCTAAAACTCCAAAATC+TGG + chr5_1:52084118-52084137 None:intergenic 35.0%
!! TTGGCCTTGTTTAACAGTAA+AGG - chr5_1:52084144-52084163 Msa0736130:CDS 35.0%
! ATACGGTTTGGATACGTTTA+TGG + chr5_1:52084201-52084220 None:intergenic 35.0%
!! TGAGTAATGTACTGGTTTCG+TGG + chr5_1:52081117-52081136 None:intergenic 40.0%
CAACGAGATGAGTAATGTAC+TGG + chr5_1:52081125-52081144 None:intergenic 40.0%
!! TCTATCAGTGTCGCCATTTT+TGG + chr5_1:52081185-52081204 None:intergenic 40.0%
! ACCAAAGGAATATGGCTTTG+TGG - chr5_1:52081211-52081230 Msa0736130:three_prime_UTR 40.0%
GGTCATGAAGAAATTGAGGA+AGG + chr5_1:52081258-52081277 None:intergenic 40.0%
! GATTCAGAAGCATAAAGGGT+TGG + chr5_1:52081414-52081433 None:intergenic 40.0%
!! GTGGAAGTGTCAATGAGTAA+TGG + chr5_1:52081502-52081521 None:intergenic 40.0%
!! CTTATTGTTTGGTTTGGCTG+TGG + chr5_1:52081521-52081540 None:intergenic 40.0%
GTGATTCCTATGTTCCTTGT+TGG - chr5_1:52081735-52081754 Msa0736130:three_prime_UTR 40.0%
GATTCCTATGTTCCTTGTTG+GGG - chr5_1:52081737-52081756 Msa0736130:three_prime_UTR 40.0%
TATACCCCAACAAGGAACAT+AGG + chr5_1:52081744-52081763 None:intergenic 40.0%
TCATCAACTATACCCCAACA+AGG + chr5_1:52081752-52081771 None:intergenic 40.0%
AAATACGCCAACAAGGCTAT+GGG + chr5_1:52081871-52081890 None:intergenic 40.0%
AAAATACGCCAACAAGGCTA+TGG + chr5_1:52081872-52081891 None:intergenic 40.0%
!!! AGCCTTGTTGGCGTATTTTT+TGG - chr5_1:52081873-52081892 Msa0736130:intron 40.0%
CACCAAAAAATACGCCAACA+AGG + chr5_1:52081878-52081897 None:intergenic 40.0%
!! CCAAAGGTGAGTTGACTTTT+GGG + chr5_1:52081906-52081925 None:intergenic 40.0%
!! TCCAAAGGTGAGTTGACTTT+TGG + chr5_1:52081907-52081926 None:intergenic 40.0%
!! TTCCAAGGTTACCGATTTGT+TGG - chr5_1:52082287-52082306 Msa0736130:intron 40.0%
TTCCAACAAATCGGTAACCT+TGG + chr5_1:52082292-52082311 None:intergenic 40.0%
!! AAGGTTACCGATTTGTTGGA+AGG - chr5_1:52082291-52082310 Msa0736130:intron 40.0%
AACACCTCCTTCCAACAAAT+CGG + chr5_1:52082301-52082320 None:intergenic 40.0%
GGTGGAGAAATATGGTCAAA+AGG - chr5_1:52082321-52082340 Msa0736130:intron 40.0%
!! TCAAAAGGCTACCGATTTGT+TGG - chr5_1:52082336-52082355 Msa0736130:intron 40.0%
ACTACCACTCACCAACAAAT+CGG + chr5_1:52082350-52082369 None:intergenic 40.0%
CACCATCTAGAAGCAAAATG+CGG + chr5_1:52082379-52082398 None:intergenic 40.0%
!!! TTTTGCTTCTAGATGGTGGA+AGG - chr5_1:52082381-52082400 Msa0736130:intron 40.0%
! GAAGGATTTTGTGAACCTAG+AGG - chr5_1:52082399-52082418 Msa0736130:intron 40.0%
! ATTTTGTGAACCTAGAGGGA+TGG - chr5_1:52082404-52082423 Msa0736130:intron 40.0%
!! GTAGTGAGGAAAGTTGGTAA+CGG - chr5_1:52082454-52082473 Msa0736130:intron 40.0%
!! AGTTGGTAACGGTACAAGTA+CGG - chr5_1:52082465-52082484 Msa0736130:intron 40.0%
!! TACAAGTACGGGTTTTTGGA+GGG - chr5_1:52082477-52082496 Msa0736130:intron 40.0%
ATCTATCCACATCTCTGTCT+TGG + chr5_1:52082567-52082586 None:intergenic 40.0%
AAGACAGAGATGTGGATAGA+TGG - chr5_1:52082566-52082585 Msa0736130:intron 40.0%
GTGGATAGATGGTTCAAATG+GGG - chr5_1:52082577-52082596 Msa0736130:intron 40.0%
TGGATAGATGGTTCAAATGG+GGG - chr5_1:52082578-52082597 Msa0736130:intron 40.0%
GGCATCAAGAATATGATAGG+TGG - chr5_1:52082690-52082709 Msa0736130:intron 40.0%
ATCGTACGAAGGATGAAAAG+AGG - chr5_1:52082795-52082814 Msa0736130:intron 40.0%
TGTGATACCAATGCGAATAC+TGG - chr5_1:52082880-52082899 Msa0736130:intron 40.0%
!!! AGTTTTTGGCTGGCAGTTTT+TGG - chr5_1:52082949-52082968 Msa0736130:intron 40.0%
! CAACAGTGTAAAGTACTCCA+AGG + chr5_1:52083343-52083362 None:intergenic 40.0%
GCTAGATACTTCTACTTCGT+AGG - chr5_1:52083362-52083381 Msa0736130:intron 40.0%
TTAATCACCCGAGTTTCAGA+CGG + chr5_1:52083412-52083431 None:intergenic 40.0%
!!! ATTTTTTATGCCGGCACTAC+TGG - chr5_1:52083567-52083586 Msa0736130:intron 40.0%
!!! TTTTTTATGCCGGCACTACT+GGG - chr5_1:52083568-52083587 Msa0736130:intron 40.0%
!! AGTTCAAATCACTGGTTTGC+AGG + chr5_1:52083640-52083659 None:intergenic 40.0%
GCAGCATATATCCCAAGTAT+GGG + chr5_1:52084229-52084248 None:intergenic 40.0%
CACAAACCAAACCTGGATTT+GGG + chr5_1:52084302-52084321 None:intergenic 40.0%
CAAAATGCACAAACCAAACC+TGG + chr5_1:52084309-52084328 None:intergenic 40.0%
GCCACAAAGCCATATTCCTT+TGG + chr5_1:52081215-52081234 None:intergenic 45.0%
CTGAACGCAATTGAAGAGCT+TGG + chr5_1:52081451-52081470 None:intergenic 45.0%
CAAGCTCTTCAATTGCGTTC+AGG - chr5_1:52081449-52081468 Msa0736130:three_prime_UTR 45.0%
AATACGCCAACAAGGCTATG+GGG + chr5_1:52081870-52081889 None:intergenic 45.0%
CCCAAAAGTCAACTCACCTT+TGG - chr5_1:52081903-52081922 Msa0736130:intron 45.0%
TATTGACCAACCTCCTAGCT+AGG - chr5_1:52081974-52081993 Msa0736130:intron 45.0%
!! ATGGTGAGAGTTGGTATGTG+CGG - chr5_1:52082192-52082211 Msa0736130:intron 45.0%
ACTTGTGAATGATGGCCCAA+CGG - chr5_1:52082253-52082272 Msa0736130:intron 45.0%
!! GTTACCGATTTGTTGGAAGG+AGG - chr5_1:52082294-52082313 Msa0736130:intron 45.0%
!! GATTTGTTGGAAGGAGGTGT+TGG - chr5_1:52082300-52082319 Msa0736130:intron 45.0%
!! GAGGTGTTGGTGGAGAAATA+TGG - chr5_1:52082313-52082332 Msa0736130:intron 45.0%
! TGCCGCATTTTGCTTCTAGA+TGG - chr5_1:52082374-52082393 Msa0736130:intron 45.0%
! CGCATTTTGCTTCTAGATGG+TGG - chr5_1:52082377-52082396 Msa0736130:intron 45.0%
! AGGGATGGCTAGCTCAAAAT+TGG - chr5_1:52082419-52082438 Msa0736130:intron 45.0%
GGTTTAACACAGAGGTAGTG+AGG - chr5_1:52082440-52082459 Msa0736130:intron 45.0%
ACAGAGGTAGTGAGGAAAGT+TGG - chr5_1:52082448-52082467 Msa0736130:intron 45.0%
!! GTTGGTAACGGTACAAGTAC+GGG - chr5_1:52082466-52082485 Msa0736130:intron 45.0%
! ACGGTACAAGTACGGGTTTT+TGG - chr5_1:52082473-52082492 Msa0736130:intron 45.0%
!! GTACAAGTACGGGTTTTTGG+AGG - chr5_1:52082476-52082495 Msa0736130:intron 45.0%
!! GAGAGGAATGGTGTCATTCA+TGG - chr5_1:52082507-52082526 Msa0736130:intron 45.0%
ATAGATGGTTCAAATGGGGG+AGG - chr5_1:52082581-52082600 Msa0736130:intron 45.0%
TAGATGGTTCAAATGGGGGA+GGG - chr5_1:52082582-52082601 Msa0736130:intron 45.0%
GAGGCGAGATTTCTTCGTAT+GGG - chr5_1:52082619-52082638 Msa0736130:intron 45.0%
! TGATAGGTGGACTTGGAAGT+TGG - chr5_1:52082703-52082722 Msa0736130:intron 45.0%
ACTTGGAAGTTGGAGGAAGT+AGG - chr5_1:52082713-52082732 Msa0736130:intron 45.0%
CTTGGAAGTTGGAGGAAGTA+GGG - chr5_1:52082714-52082733 Msa0736130:intron 45.0%
! TGGAGGAAGTAGGGAGTTTT+TGG - chr5_1:52082723-52082742 Msa0736130:intron 45.0%
! GGAGGAAGTAGGGAGTTTTT+GGG - chr5_1:52082724-52082743 Msa0736130:intron 45.0%
TGTGAAGTTGGAAGGCTTGA+TGG - chr5_1:52082760-52082779 Msa0736130:intron 45.0%
! AGTTGGAAGGCTTGATGGTA+AGG - chr5_1:52082765-52082784 Msa0736130:intron 45.0%
! GTTGGAAGGCTTGATGGTAA+GGG - chr5_1:52082766-52082785 Msa0736130:intron 45.0%
CAAAAACTGCCAGAAACACG+CGG + chr5_1:52082937-52082956 None:intergenic 45.0%
!!! TGTTTCTGGCAGTTTTTGGC+TGG - chr5_1:52082939-52082958 Msa0736130:intron 45.0%
!! TTTTTGGCCATTGGCTGTTG+CGG - chr5_1:52082965-52082984 Msa0736130:intron 45.0%
TGTTGCGGTTTCCTTTCCTT+CGG - chr5_1:52082980-52082999 Msa0736130:intron 45.0%
! CTCTAAGTTCTAGCGGTTGT+GGG - chr5_1:52083008-52083027 Msa0736130:intron 45.0%
! TCTAAGTTCTAGCGGTTGTG+GGG - chr5_1:52083009-52083028 Msa0736130:intron 45.0%
! GCATCGTTTCCAGAGAGTTT+TGG + chr5_1:52083262-52083281 None:intergenic 45.0%
CCAAGGACAGCACAAATGAT+AGG + chr5_1:52083326-52083345 None:intergenic 45.0%
CCTATCATTTGTGCTGTCCT+TGG - chr5_1:52083323-52083342 Msa0736130:intron 45.0%
! TTTTTATGCCGGCACTACTG+GGG - chr5_1:52083569-52083588 Msa0736130:intron 45.0%
!! ACCATAGCCTCTTGATGATG+AGG - chr5_1:52083672-52083691 Msa0736130:intron 45.0%
ACCTCATCATCAAGAGGCTA+TGG + chr5_1:52083676-52083695 None:intergenic 45.0%
AATTGACGACTACACTCTGC+AGG + chr5_1:52083922-52083941 None:intergenic 45.0%
GAGTGTAGTCGTCAATTCAC+AGG - chr5_1:52083925-52083944 Msa0736130:intron 45.0%
GTATGGGTGCAAATACGGTT+TGG + chr5_1:52084213-52084232 None:intergenic 45.0%
ACCGTATTTGCACCCATACT+TGG - chr5_1:52084214-52084233 Msa0736130:CDS 45.0%
CCCAAGTATGGGTGCAAATA+CGG + chr5_1:52084218-52084237 None:intergenic 45.0%
CCGTATTTGCACCCATACTT+GGG - chr5_1:52084215-52084234 Msa0736130:CDS 45.0%
CGCAGCATATATCCCAAGTA+TGG + chr5_1:52084230-52084249 None:intergenic 45.0%
AGTTCGTATCTCCCAAATCC+AGG - chr5_1:52084288-52084307 Msa0736130:five_prime_UTR 45.0%
GTATCTCCCAAATCCAGGTT+TGG - chr5_1:52084293-52084312 Msa0736130:five_prime_UTR 45.0%
GCACAAACCAAACCTGGATT+TGG + chr5_1:52084303-52084322 None:intergenic 45.0%
GCGAGCTTTGCCAACTTGAA+AGG + chr5_1:52081279-52081298 None:intergenic 50.0%
AATGTTGGTCGATGGGGTGA+TGG - chr5_1:52082063-52082082 Msa0736130:CDS 50.0%
!!! TGAGAAGGCTTTTGACTCGG+TGG - chr5_1:52082146-52082165 Msa0736130:CDS 50.0%
!!! GAGAAGGCTTTTGACTCGGT+GGG - chr5_1:52082147-52082166 Msa0736130:CDS 50.0%
!! TTGTTGGAAGGAGGTGTTGG+TGG - chr5_1:52082303-52082322 Msa0736130:intron 50.0%
!! GCTACCGATTTGTTGGTGAG+TGG - chr5_1:52082343-52082362 Msa0736130:intron 50.0%
TTTGAGCTAGCCATCCCTCT+AGG + chr5_1:52082417-52082436 None:intergenic 50.0%
!! AAGTACGGGTTTTTGGAGGG+TGG - chr5_1:52082480-52082499 Msa0736130:intron 50.0%
GGAGGCGAGATTTCTTCGTA+TGG - chr5_1:52082618-52082637 Msa0736130:intron 50.0%
! TAGGTGGACTTGGAAGTTGG+AGG - chr5_1:52082706-52082725 Msa0736130:intron 50.0%
AAGGGAGGACAATCGTACGA+AGG - chr5_1:52082784-52082803 Msa0736130:intron 50.0%
CATTACTGACCGCGTGTTTC+TGG - chr5_1:52082925-52082944 Msa0736130:intron 50.0%
!!! CGCGTGTTTCTGGCAGTTTT+TGG - chr5_1:52082935-52082954 Msa0736130:intron 50.0%
AAGGAAACCGCAACAGCCAA+TGG + chr5_1:52082975-52082994 None:intergenic 50.0%
GTTGCGGTTTCCTTTCCTTC+GGG - chr5_1:52082981-52083000 Msa0736130:intron 50.0%
TTGCGGTTTCCTTTCCTTCG+GGG - chr5_1:52082982-52083001 Msa0736130:intron 50.0%
! GCTCTAAGTTCTAGCGGTTG+TGG - chr5_1:52083007-52083026 Msa0736130:intron 50.0%
! TGTGTTTGGTGAGTGCAGAG+AGG - chr5_1:52083233-52083252 Msa0736130:intron 50.0%
! GTGTTTGGTGAGTGCAGAGA+GGG - chr5_1:52083234-52083253 Msa0736130:intron 50.0%
TACTTCGTAGGCTATGCCAC+AGG - chr5_1:52083374-52083393 Msa0736130:intron 50.0%
GGGCAAAGCCCAATCAATCA+TGG + chr5_1:52083475-52083494 None:intergenic 50.0%
CAGATGTCTCATTGCGGACT+TGG - chr5_1:52083972-52083991 Msa0736130:CDS 50.0%
GAACCTTGGCCATGTACTAG+TGG - chr5_1:52084026-52084045 Msa0736130:intron 50.0%
TGACCACTAGTACATGGCCA+AGG + chr5_1:52084032-52084051 None:intergenic 50.0%
TGACCAACCTCCTAGCTAGG+AGG - chr5_1:52081977-52081996 Msa0736130:intron 55.0%
CAACCTCCTAGCTAGGAGGT+TGG + chr5_1:52081981-52082000 None:intergenic 55.0%
CAACCTCCTAGCTAGGAGGT+TGG - chr5_1:52081981-52082000 Msa0736130:intron 55.0%
TTGCCAACCTCCTAGCTAGG+AGG + chr5_1:52081987-52082006 None:intergenic 55.0%
CACTTGCCAACCTCCTAGCT+AGG + chr5_1:52081990-52082009 None:intergenic 55.0%
AGCTAGGAGGTTGGCAAGTG+TGG - chr5_1:52081990-52082009 Msa0736130:intron 55.0%
!!! AGGCTTTTGACTCGGTGGGT+TGG - chr5_1:52082151-52082170 Msa0736130:CDS 55.0%
!!! CTTTTGACTCGGTGGGTTGG+AGG - chr5_1:52082154-52082173 Msa0736130:CDS 55.0%
!!! CTCGGTGGGTTGGAGGTTTT+TGG - chr5_1:52082161-52082180 Msa0736130:intron 55.0%
TGCGGTTGAGTGTGTGTGTG+TGG - chr5_1:52082210-52082229 Msa0736130:intron 55.0%
GCGGTTGAGTGTGTGTGTGT+GGG - chr5_1:52082211-52082230 Msa0736130:intron 55.0%
CGGTTGAGTGTGTGTGTGTG+GGG - chr5_1:52082212-52082231 Msa0736130:intron 55.0%
TTGAGTGTGTGTGTGTGGGG+AGG - chr5_1:52082215-52082234 Msa0736130:intron 55.0%
TGAGTGTGTGTGTGTGGGGA+GGG - chr5_1:52082216-52082235 Msa0736130:intron 55.0%
ATGGTTCAAATGGGGGAGGG+TGG - chr5_1:52082585-52082604 Msa0736130:intron 55.0%
TGGTTCAAATGGGGGAGGGT+GGG - chr5_1:52082586-52082605 Msa0736130:intron 55.0%
! GGAAGGCTTGATGGTAAGGG+AGG - chr5_1:52082769-52082788 Msa0736130:intron 55.0%
!!! GGCTGGCAGTTTTTGGCCAT+TGG - chr5_1:52082956-52082975 Msa0736130:intron 55.0%
TTAGAGCTGCCCCGAAGGAA+AGG + chr5_1:52082994-52083013 None:intergenic 55.0%
AGAACTTAGAGCTGCCCCGA+AGG + chr5_1:52082999-52083018 None:intergenic 55.0%
!! GGGGCAGCTCTAAGTTCTAG+CGG - chr5_1:52083001-52083020 Msa0736130:intron 55.0%
! AGTTCTAGCGGTTGTGGGGA+TGG - chr5_1:52083013-52083032 Msa0736130:intron 55.0%
GTTCTAGCGGTTGTGGGGAT+GGG - chr5_1:52083014-52083033 Msa0736130:intron 55.0%
!! CGGTTGTGGGGATGGGTTTT+TGG - chr5_1:52083021-52083040 Msa0736130:intron 55.0%
!! GAGAGGGCACCAAAACTCTC+TGG - chr5_1:52083250-52083269 Msa0736130:intron 55.0%
CCGAGTTTCAGACGGTTCTC+GGG + chr5_1:52083404-52083423 None:intergenic 55.0%
! CCCGAGAACCGTCTGAAACT+CGG - chr5_1:52083401-52083420 Msa0736130:intron 55.0%
CCCGAGTTTCAGACGGTTCT+CGG + chr5_1:52083405-52083424 None:intergenic 55.0%
! CCGAGAACCGTCTGAAACTC+GGG - chr5_1:52083402-52083421 Msa0736130:intron 55.0%
ACCATAAGCCCCAGTAGTGC+CGG + chr5_1:52083580-52083599 None:intergenic 55.0%
TGGGGACAGCCTCATTGCCA+TGG + chr5_1:52081852-52081871 None:intergenic 60.0%
!! GGCTGTCCCCATAGCCTTGT+TGG - chr5_1:52081861-52081880 Msa0736130:intron 60.0%
GAGTGTGTGTGTGTGGGGAG+GGG - chr5_1:52082217-52082236 Msa0736130:intron 60.0%
AGTGTGTGTGTGTGGGGAGG+GGG - chr5_1:52082218-52082237 Msa0736130:intron 60.0%
!! TTTTGGAGGGTGGCGTGGAG+AGG - chr5_1:52082490-52082509 Msa0736130:intron 60.0%
GGTTCAAATGGGGGAGGGTG+GGG - chr5_1:52082587-52082606 Msa0736130:intron 60.0%
GTTCAAATGGGGGAGGGTGG+GGG - chr5_1:52082588-52082607 Msa0736130:intron 60.0%
! GAGGGTGGGGGTTTTCTTGG+AGG - chr5_1:52082600-52082619 Msa0736130:intron 60.0%
ACGGTTCTCGGGTTTGCCTG+TGG + chr5_1:52083393-52083412 None:intergenic 60.0%
GTGTGTGTGTGTGGGGAGGG+GGG - chr5_1:52082219-52082238 Msa0736130:intron 65.0%
!! CGGGTTTTTGGAGGGTGGCG+TGG - chr5_1:52082485-52082504 Msa0736130:intron 65.0%
GAGGGTGGCGTGGAGAGGAA+TGG - chr5_1:52082495-52082514 Msa0736130:intron 65.0%
! GGGGAGGGTGGGGGTTTTCT+TGG - chr5_1:52082597-52082616 Msa0736130:intron 65.0%
GCCGGCACTACTGGGGCTTA+TGG - chr5_1:52083576-52083595 Msa0736130:intron 65.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 52081071 52084356 52081071 ID=Msa0736130;Name=Msa0736130
chr5_1 mRNA 52081071 52084356 52081071 ID=Msa0736130-mRNA-1;Parent=Msa0736130;Name=Msa0736130-mRNA-1;_AED=0.01;_eAED=0.01;_QI=117|1|1|1|1|1|4|705|150
chr5_1 exon 52081071 52081863 52081071 ID=Msa0736130-mRNA-1:exon:14710;Parent=Msa0736130-mRNA-1
chr5_1 exon 52082004 52082176 52082004 ID=Msa0736130-mRNA-1:exon:14709;Parent=Msa0736130-mRNA-1
chr5_1 exon 52083957 52084032 52083957 ID=Msa0736130-mRNA-1:exon:14708;Parent=Msa0736130-mRNA-1
chr5_1 exon 52084124 52084356 52084124 ID=Msa0736130-mRNA-1:exon:14707;Parent=Msa0736130-mRNA-1
chr5_1 five_prime_UTR 52084240 52084356 52084240 ID=Msa0736130-mRNA-1:five_prime_utr;Parent=Msa0736130-mRNA-1
chr5_1 CDS 52084124 52084239 52084124 ID=Msa0736130-mRNA-1:cds;Parent=Msa0736130-mRNA-1
chr5_1 CDS 52083957 52084032 52083957 ID=Msa0736130-mRNA-1:cds;Parent=Msa0736130-mRNA-1
chr5_1 CDS 52082004 52082176 52082004 ID=Msa0736130-mRNA-1:cds;Parent=Msa0736130-mRNA-1
chr5_1 CDS 52081776 52081863 52081776 ID=Msa0736130-mRNA-1:cds;Parent=Msa0736130-mRNA-1
chr5_1 three_prime_UTR 52081071 52081775 52081071 ID=Msa0736130-mRNA-1:three_prime_utr;Parent=Msa0736130-mRNA-1
Gene Sequence

>Msa0736130

ATGGCGACACTGATAGAAATGTTACCAAAGGAATATGGCTTTGTGGCAATCGTTCTCGTTCTCTACACCTTCCTCAATTTCTTCATGACCTTTCAAGTTGGCAAAGCTCGCAAGAAGTACAAAGTTTTTTATCCAACCCTTTATGCTTCTGAATCTGAAAACAAAGATGCCAAGCTCTTCAATTGCGTTCAGAGAGGGCACCAAAACTCTCTGGAAACGATGCCCATTTTCTTCATGTTGATGATTTTGGGAGGGCTGAAGCATCCTATCATTTGTGCTGTCCTTGGAGTACTTTACACTGTTGCTAGATACTTCTACTTCGTAGGCTATGCCACAGGCAAACCCGAGAACCGTCTGAAACTCGGGGGATTTTTTATGCCGGCACTACTGGGGCTTATGGTGTCTACACTTTCATTTGGATGGAGTCTTGTCAATCAGCCTGCAAACCAGTGA
Protein sequence

>Msa0736130

MATLIEMLPKEYGFVAIVLVLYTFLNFFMTFQVGKARKKYKVFYPTLYASESENKDAKLFNCVQRGHQNSLETMPIFFMLMILGGLKHPIICAVLGVLYTVARYFYFVGYATGKPENRLKLGGFFMPALLGLMVSTLSFGWSLVNQPANQ*