AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0736540

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0736540 MtrunA17_Chr5g0433951 96.988 166 5 0 1 166 1 166 1.43e-118 332
Msa0736540 MtrunA17_Chr4g0008801 54.545 165 75 0 1 165 1 165 5.20e-60 183
Msa0736540 MtrunA17_Chr4g0032261 54.777 157 71 0 1 157 1 157 1.08e-58 180
Msa0736540 MtrunA17_Chr7g0240701 49.045 157 80 0 1 157 1 157 5.50e-54 168
Msa0736540 MtrunA17_Chr2g0294421 50.318 157 78 0 1 157 1 157 6.01e-51 160
Msa0736540 MtrunA17_Chr1g0194801 35.849 159 86 6 9 161 10 158 3.29e-20 82.0
Msa0736540 MtrunA17_Chr5g0427291 37.349 166 88 5 6 158 9 171 8.52e-19 79.0
Msa0736540 MtrunA17_Chr1g0192651 35.714 154 83 6 9 157 13 155 1.06e-18 78.2
Msa0736540 MtrunA17_Chr4g0001641 56.140 57 25 0 102 158 10 66 1.24e-17 72.8
Msa0736540 MtrunA17_Chr1g0176311 27.128 188 88 5 7 166 24 190 8.28e-17 73.9
Msa0736540 MtrunA17_Chr1g0176321 27.513 189 92 4 7 166 5 177 6.65e-16 71.2
Msa0736540 MtrunA17_Chr6g0450171 29.932 147 92 3 14 157 24 162 1.63e-14 67.4
Msa0736540 MtrunA17_Chr2g0323291 30.435 161 96 4 7 157 27 181 8.36e-14 66.6
Msa0736540 MtrunA17_Chr1g0195661 28.387 155 96 5 9 157 16 161 2.60e-12 61.6
Msa0736540 MtrunA17_Chr6g0464761 50.000 56 21 1 98 153 15 63 3.69e-12 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0736540 AT3G03270.2 70.701 157 46 0 1 157 1 157 4.24e-79 232
Msa0736540 AT3G03270.1 68.657 134 42 0 1 134 1 134 3.98e-63 193
Msa0736540 AT3G53990.1 50.318 157 78 0 1 157 1 157 5.43e-54 168
Msa0736540 AT3G17020.1 46.711 152 80 1 7 157 9 160 7.05e-49 155
Msa0736540 AT3G53990.2 42.857 98 56 0 1 98 1 98 3.82e-24 91.7
Msa0736540 AT1G09740.2 37.736 159 87 4 7 157 10 164 3.27e-19 80.1
Msa0736540 AT1G09740.1 37.736 159 87 4 7 157 10 164 3.27e-19 80.1
Msa0736540 AT3G62550.1 34.356 163 88 5 3 157 5 156 6.57e-18 76.6
Msa0736540 AT3G58450.2 32.317 164 84 6 7 157 32 181 1.87e-17 76.3
Msa0736540 AT3G17020.2 37.079 89 55 1 7 94 9 97 7.70e-17 72.4
Msa0736540 AT3G58450.1 32.544 169 84 6 7 157 32 188 1.51e-16 73.9
Msa0736540 AT1G11360.2 29.870 154 105 1 7 157 40 193 3.45e-16 73.6
Msa0736540 AT1G11360.1 29.870 154 105 1 7 157 40 193 3.45e-16 73.6
Msa0736540 AT1G11360.4 29.870 154 105 1 7 157 40 193 3.45e-16 73.6
Msa0736540 AT1G11360.3 29.870 154 105 1 7 157 40 193 3.45e-16 73.6
Msa0736540 AT1G68300.1 31.818 154 94 4 7 157 12 157 1.91e-15 70.1
Msa0736540 AT3G01520.1 29.252 147 93 3 14 157 24 162 2.05e-15 70.1
Msa0736540 AT5G14680.1 28.571 147 94 3 14 157 24 162 1.02e-14 68.6
Msa0736540 AT3G11930.4 29.560 159 101 2 9 157 37 194 1.12e-14 68.9
Msa0736540 AT3G11930.1 29.560 159 99 3 9 157 37 192 2.14e-14 68.2
Msa0736540 AT3G11930.2 29.560 159 100 3 9 157 37 193 4.95e-14 67.0
Msa0736540 AT5G54430.1 29.932 147 88 3 7 144 50 190 6.44e-11 59.3
Msa0736540 AT5G54430.4 29.932 147 88 3 7 144 50 190 6.44e-11 59.3
Msa0736540 AT5G54430.3 29.932 147 88 3 7 144 50 190 7.13e-11 59.3
Msa0736540 AT5G54430.6 29.932 147 88 3 7 144 50 190 7.13e-11 59.3
Msa0736540 AT5G54430.2 29.932 147 88 3 7 144 50 190 7.25e-11 59.3
Msa0736540 AT5G54430.5 29.932 147 88 3 7 144 50 190 7.25e-11 59.3

Find 57 sgRNAs with CRISPR-Local

Find 202 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGTGCCTCTAGAGGAATTA+AGG 0.233780 5_1:-53073126 Msa0736540:CDS
CATCTGGACTCATTGGTTAT+TGG 0.247760 5_1:-53072660 Msa0736540:CDS
GGTAGTGGCAAAGGTGTATT+GGG 0.310523 5_1:-53072724 Msa0736540:CDS
ATGATTATTAATATTTACTT+TGG 0.311087 5_1:-53072395 Msa0736540:three_prime_UTR
CTTCATTTCAGTGTGTTGTT+AGG 0.317283 5_1:-53072509 Msa0736540:intron
GTTATTGGAAGCAGGGGTTT+AGG 0.321337 5_1:-53072645 Msa0736540:CDS
CCATCTAAGTGCTAATTTAC+TGG 0.336238 5_1:+53073728 None:intergenic
CCAGTAAATTAGCACTTAGA+TGG 0.339331 5_1:-53073728 Msa0736540:CDS
GGGGTTTAGGTTCCATTAAA+AGG 0.346706 5_1:-53072632 Msa0736540:intron
TCAAGAATATCTATGACTTC+AGG 0.358570 5_1:+53073064 None:intergenic
AGATCTTCATCTGGACTCAT+TGG 0.398485 5_1:-53072667 Msa0736540:CDS
CATGAAACAATTGTCACATA+AGG 0.399976 5_1:-53072144 Msa0736540:three_prime_UTR
AAACAACATAAATACCCCTT+TGG 0.402357 5_1:+53073027 None:intergenic
ATAAACTTTACAAAGCAATA+TGG 0.410129 5_1:-53073100 Msa0736540:CDS
ATTCCCTTGACTACAGTAAC+CGG 0.414500 5_1:+53072449 None:intergenic
TGATTATTAATATTTACTTT+GGG 0.421374 5_1:-53072394 Msa0736540:three_prime_UTR
TGGTAGTGGCAAAGGTGTAT+TGG 0.429624 5_1:-53072725 Msa0736540:CDS
TGTGATCAGCTGAGGGAGGC+TGG 0.432159 5_1:+53073655 None:intergenic
GCATTGCACAATTTCTCCCT+TGG 0.434268 5_1:+53072696 None:intergenic
GGTGCCTCTAGAGGAATTAA+GGG 0.447240 5_1:-53073125 Msa0736540:CDS
TGCTGTGCAAGATCTTCATC+TGG 0.451178 5_1:-53072676 Msa0736540:CDS
ACTCAGATAAATTGTAATGA+TGG 0.464485 5_1:+53072245 None:intergenic
ATGAAACAATTGTCACATAA+GGG 0.468956 5_1:-53072143 Msa0736540:three_prime_UTR
CTCATTGGTTATTGGAAGCA+GGG 0.470129 5_1:-53072652 Msa0736540:CDS
ACTCATTGGTTATTGGAAGC+AGG 0.470238 5_1:-53072653 Msa0736540:CDS
CTTGAACCAGGCAATGGTAG+TGG 0.472382 5_1:-53072739 Msa0736540:intron
ATGGCAAAAGCACACATAGT+TGG 0.510567 5_1:-53073774 Msa0736540:CDS
TTCCTAGGAGTATTGTGCCT+CGG 0.514550 5_1:+53073800 None:intergenic
ATCAAATCAATGCCTAGACT+TGG 0.522276 5_1:+53072418 None:intergenic
CTTTCTAGTGTGATCAGCTG+AGG 0.523922 5_1:+53073647 None:intergenic
CTCAGCTGATCACACTAGAA+AGG 0.525726 5_1:-53073646 Msa0736540:CDS
ACAATACTCCTAGGAAAAGA+TGG 0.525752 5_1:-53073793 Msa0736540:exon
GCTAGCCAAAGCATTTCAAA+AGG 0.531277 5_1:+53072329 None:intergenic
TGCAGCTTTGGTGCCTCTAG+AGG 0.535834 5_1:-53073134 Msa0736540:intron
CCAGGCAATGGTAGTGGCAA+AGG 0.540554 5_1:-53072733 Msa0736540:intron
ATCTCCCTTAATTCCTCTAG+AGG 0.547571 5_1:+53073121 None:intergenic
TTCCGAGGCACAATACTCCT+AGG 0.555541 5_1:-53073802 Msa0736540:five_prime_UTR
TTGCCCGGTTACTGTAGTCA+AGG 0.560158 5_1:-53072453 Msa0736540:CDS
AGGTGTATTGGGGAGATCCA+AGG 0.560692 5_1:-53072713 Msa0736540:CDS
ACACATAGTTGGAGTAGCTA+TGG 0.571440 5_1:-53073763 Msa0736540:CDS
GAATGCAACCTTCCAAGTCT+AGG 0.571630 5_1:-53072430 Msa0736540:CDS
AAAGCAATATGGAATTGCTA+AGG 0.573242 5_1:-53073089 Msa0736540:CDS
TGCCCGGTTACTGTAGTCAA+GGG 0.580340 5_1:-53072452 Msa0736540:CDS
CTAAGTGCTAATTTACTGGT+AGG 0.583052 5_1:+53073732 None:intergenic
TTTCTAGTGTGATCAGCTGA+GGG 0.583250 5_1:+53073648 None:intergenic
GGTGACAAATGCTTCTTGCC+CGG 0.585729 5_1:-53072468 Msa0736540:CDS
CAGTAAATTAGCACTTAGAT+GGG 0.588277 5_1:-53073727 Msa0736540:CDS
AATCAATGCCTAGACTTGGA+AGG 0.590106 5_1:+53072422 None:intergenic
GGTGTATTGGGGAGATCCAA+GGG 0.593103 5_1:-53072712 Msa0736540:CDS
ATTGTGCCTCGGAATTTCAG+TGG 0.603572 5_1:+53073811 None:intergenic
CTAGTGTGATCAGCTGAGGG+AGG 0.611787 5_1:+53073651 None:intergenic
TCATTGGTTATTGGAAGCAG+GGG 0.616498 5_1:-53072651 Msa0736540:CDS
TTCCCTTGACTACAGTAACC+GGG 0.638321 5_1:+53072450 None:intergenic
AAGAAACCACTGAAATTCCG+AGG 0.659277 5_1:-53073817 Msa0736540:five_prime_UTR
GTAGTGGCAAAGGTGTATTG+GGG 0.678633 5_1:-53072723 Msa0736540:CDS
ATGTGTCATATATTGATGAG+AGG 0.679189 5_1:-53072195 Msa0736540:three_prime_UTR
AGGCAGTGTTAGCAAGCATG+TGG 0.730937 5_1:-53072489 Msa0736540:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGATTATTAATATTTACTT+TGG - chr5_1:53073552-53073571 Msa0736540:intron 10.0%
!! TGATTATTAATATTTACTTT+GGG - chr5_1:53073553-53073572 Msa0736540:intron 10.0%
!! ATGATTATTAATATTTACTT+TGG - chr5_1:53073552-53073571 Msa0736540:intron 10.0%
!! TGATTATTAATATTTACTTT+GGG - chr5_1:53073553-53073572 Msa0736540:intron 10.0%
!!! TTTTTTTCTCATGTTATTAT+CGG + chr5_1:53073045-53073064 None:intergenic 15.0%
!!! TTTTTTTCTCATGTTATTAT+CGG + chr5_1:53073045-53073064 None:intergenic 15.0%
!! ATAAACTTTACAAAGCAATA+TGG - chr5_1:53072847-53072866 Msa0736540:intron 20.0%
!!! TAAACTATAGTACCTTTTAA+TGG + chr5_1:53073330-53073349 None:intergenic 20.0%
!! ATAAACTTTACAAAGCAATA+TGG - chr5_1:53072847-53072866 Msa0736540:intron 20.0%
!!! TAAACTATAGTACCTTTTAA+TGG + chr5_1:53073330-53073349 None:intergenic 20.0%
! TAAAAGAAAGAGAATGAAAG+AGG + chr5_1:53072358-53072377 None:intergenic 25.0%
!! ACTGATGTTATTTAAAAGCT+TGG + chr5_1:53072392-53072411 None:intergenic 25.0%
!! AAGATTTTAAATAGCAGAAG+TGG + chr5_1:53072595-53072614 None:intergenic 25.0%
! TATGTTGTATAGGTTTATCT+TGG - chr5_1:53072731-53072750 Msa0736540:CDS 25.0%
!!! TGACAATTTTGTGTTTTGTT+TGG - chr5_1:53072781-53072800 Msa0736540:intron 25.0%
!!! TTTTTTATGTGTGTCTTTGT+TGG - chr5_1:53073182-53073201 Msa0736540:intron 25.0%
!! AGCTTGAATAATTGTCTTTT+TGG - chr5_1:53073638-53073657 Msa0736540:CDS 25.0%
! ACTCAGATAAATTGTAATGA+TGG + chr5_1:53073705-53073724 None:intergenic 25.0%
! ATGAAACAATTGTCACATAA+GGG - chr5_1:53073804-53073823 Msa0736540:five_prime_UTR 25.0%
! TAAAAGAAAGAGAATGAAAG+AGG + chr5_1:53072358-53072377 None:intergenic 25.0%
!! ACTGATGTTATTTAAAAGCT+TGG + chr5_1:53072392-53072411 None:intergenic 25.0%
!! AAGATTTTAAATAGCAGAAG+TGG + chr5_1:53072595-53072614 None:intergenic 25.0%
! TATGTTGTATAGGTTTATCT+TGG - chr5_1:53072731-53072750 Msa0736540:CDS 25.0%
!!! TGACAATTTTGTGTTTTGTT+TGG - chr5_1:53072781-53072800 Msa0736540:intron 25.0%
!!! TTTTTTATGTGTGTCTTTGT+TGG - chr5_1:53073182-53073201 Msa0736540:intron 25.0%
!! AGCTTGAATAATTGTCTTTT+TGG - chr5_1:53073638-53073657 Msa0736540:CDS 25.0%
! ACTCAGATAAATTGTAATGA+TGG + chr5_1:53073705-53073724 None:intergenic 25.0%
! ATGAAACAATTGTCACATAA+GGG - chr5_1:53073804-53073823 Msa0736540:five_prime_UTR 25.0%
CAGTAAATTAGCACTTAGAT+GGG - chr5_1:53072220-53072239 Msa0736540:three_prime_UTR 30.0%
CTGTTAAATTTATACGACTG+TGG - chr5_1:53072447-53072466 Msa0736540:CDS 30.0%
TTCATAACTACAGAAAACCA+CGG + chr5_1:53072546-53072565 None:intergenic 30.0%
TGCTGAAACAATCTCATTTA+TGG - chr5_1:53072615-53072634 Msa0736540:intron 30.0%
! GATTTTTGATGTCTCAACAA+CGG + chr5_1:53072647-53072666 None:intergenic 30.0%
AAAATCCATGAATGCAATCT+GGG + chr5_1:53072682-53072701 None:intergenic 30.0%
AAAAATCCATGAATGCAATC+TGG + chr5_1:53072683-53072702 None:intergenic 30.0%
GCACATACTATATGTTGTAT+AGG - chr5_1:53072721-53072740 Msa0736540:CDS 30.0%
AAAGCAATATGGAATTGCTA+AGG - chr5_1:53072858-53072877 Msa0736540:intron 30.0%
TCAAGAATATCTATGACTTC+AGG + chr5_1:53072886-53072905 None:intergenic 30.0%
AAACAACATAAATACCCCTT+TGG + chr5_1:53072923-53072942 None:intergenic 30.0%
TGGTGTAGTTAACACATATA+TGG - chr5_1:53073155-53073174 Msa0736540:intron 30.0%
!! GAATAATTGTCTTTTTGGCA+TGG - chr5_1:53073643-53073662 Msa0736540:CDS 30.0%
ATGTGTCATATATTGATGAG+AGG - chr5_1:53073752-53073771 Msa0736540:CDS 30.0%
CATGAAACAATTGTCACATA+AGG - chr5_1:53073803-53073822 Msa0736540:five_prime_UTR 30.0%
CAGTAAATTAGCACTTAGAT+GGG - chr5_1:53072220-53072239 Msa0736540:three_prime_UTR 30.0%
CTGTTAAATTTATACGACTG+TGG - chr5_1:53072447-53072466 Msa0736540:CDS 30.0%
TTCATAACTACAGAAAACCA+CGG + chr5_1:53072546-53072565 None:intergenic 30.0%
TGCTGAAACAATCTCATTTA+TGG - chr5_1:53072615-53072634 Msa0736540:intron 30.0%
! GATTTTTGATGTCTCAACAA+CGG + chr5_1:53072647-53072666 None:intergenic 30.0%
AAAATCCATGAATGCAATCT+GGG + chr5_1:53072682-53072701 None:intergenic 30.0%
AAAAATCCATGAATGCAATC+TGG + chr5_1:53072683-53072702 None:intergenic 30.0%
GCACATACTATATGTTGTAT+AGG - chr5_1:53072721-53072740 Msa0736540:CDS 30.0%
AAAGCAATATGGAATTGCTA+AGG - chr5_1:53072858-53072877 Msa0736540:intron 30.0%
TCAAGAATATCTATGACTTC+AGG + chr5_1:53072886-53072905 None:intergenic 30.0%
AAACAACATAAATACCCCTT+TGG + chr5_1:53072923-53072942 None:intergenic 30.0%
TGGTGTAGTTAACACATATA+TGG - chr5_1:53073155-53073174 Msa0736540:intron 30.0%
!! GAATAATTGTCTTTTTGGCA+TGG - chr5_1:53073643-53073662 Msa0736540:CDS 30.0%
ATGTGTCATATATTGATGAG+AGG - chr5_1:53073752-53073771 Msa0736540:CDS 30.0%
CATGAAACAATTGTCACATA+AGG - chr5_1:53073803-53073822 Msa0736540:five_prime_UTR 30.0%
ACAATACTCCTAGGAAAAGA+TGG - chr5_1:53072154-53072173 Msa0736540:three_prime_UTR 35.0%
CTAAGTGCTAATTTACTGGT+AGG + chr5_1:53072218-53072237 None:intergenic 35.0%
CCATCTAAGTGCTAATTTAC+TGG + chr5_1:53072222-53072241 None:intergenic 35.0%
CCAGTAAATTAGCACTTAGA+TGG - chr5_1:53072219-53072238 Msa0736540:three_prime_UTR 35.0%
AAATTTATACGACTGTGGTC+TGG - chr5_1:53072452-53072471 Msa0736540:CDS 35.0%
!! AGTCGATTTTGCCTCAATTA+TGG + chr5_1:53072515-53072534 None:intergenic 35.0%
CATATAGTATGTGCTAAGCA+AGG + chr5_1:53072715-53072734 None:intergenic 35.0%
AAACTGCCTCAAAAACCAAA+GGG - chr5_1:53072905-53072924 Msa0736540:intron 35.0%
!!! AAATACCCCTTTGGTTTTTG+AGG + chr5_1:53072914-53072933 None:intergenic 35.0%
! CTTCATTTCAGTGTGTTGTT+AGG - chr5_1:53073438-53073457 Msa0736540:intron 35.0%
ATCAAATCAATGCCTAGACT+TGG + chr5_1:53073532-53073551 None:intergenic 35.0%
!!! CTGTACCTTTTGAAATGCTT+TGG - chr5_1:53073613-53073632 Msa0736540:intron 35.0%
ACAATACTCCTAGGAAAAGA+TGG - chr5_1:53072154-53072173 Msa0736540:three_prime_UTR 35.0%
CTAAGTGCTAATTTACTGGT+AGG + chr5_1:53072218-53072237 None:intergenic 35.0%
CCATCTAAGTGCTAATTTAC+TGG + chr5_1:53072222-53072241 None:intergenic 35.0%
CCAGTAAATTAGCACTTAGA+TGG - chr5_1:53072219-53072238 Msa0736540:three_prime_UTR 35.0%
AAATTTATACGACTGTGGTC+TGG - chr5_1:53072452-53072471 Msa0736540:CDS 35.0%
!! AGTCGATTTTGCCTCAATTA+TGG + chr5_1:53072515-53072534 None:intergenic 35.0%
CATATAGTATGTGCTAAGCA+AGG + chr5_1:53072715-53072734 None:intergenic 35.0%
AAACTGCCTCAAAAACCAAA+GGG - chr5_1:53072905-53072924 Msa0736540:intron 35.0%
!!! AAATACCCCTTTGGTTTTTG+AGG + chr5_1:53072914-53072933 None:intergenic 35.0%
! CTTCATTTCAGTGTGTTGTT+AGG - chr5_1:53073438-53073457 Msa0736540:intron 35.0%
ATCAAATCAATGCCTAGACT+TGG + chr5_1:53073532-53073551 None:intergenic 35.0%
!!! CTGTACCTTTTGAAATGCTT+TGG - chr5_1:53073613-53073632 Msa0736540:intron 35.0%
! TGCTTTTGCCATCTTTTCCT+AGG + chr5_1:53072165-53072184 None:intergenic 40.0%
! ATGGCAAAAGCACACATAGT+TGG - chr5_1:53072173-53072192 Msa0736540:three_prime_UTR 40.0%
ACACATAGTTGGAGTAGCTA+TGG - chr5_1:53072184-53072203 Msa0736540:three_prime_UTR 40.0%
! TTTCTAGTGTGATCAGCTGA+GGG + chr5_1:53072302-53072321 None:intergenic 40.0%
!! AAGGAGCTTTTTGAAGACAC+TGG - chr5_1:53072320-53072339 Msa0736540:three_prime_UTR 40.0%
TGTCTCTACGACCATAATTG+AGG - chr5_1:53072501-53072520 Msa0736540:CDS 40.0%
AAATCGACTATAAGTGACCG+TGG - chr5_1:53072526-53072545 Msa0736540:intron 40.0%
!! TGGTTGATGATTTGCAGCTT+TGG - chr5_1:53072801-53072820 Msa0736540:intron 40.0%
ATCTCCCTTAATTCCTCTAG+AGG + chr5_1:53072829-53072848 None:intergenic 40.0%
GAAACTGCCTCAAAAACCAA+AGG - chr5_1:53072904-53072923 Msa0736540:intron 40.0%
AACTGCCTCAAAAACCAAAG+GGG - chr5_1:53072906-53072925 Msa0736540:intron 40.0%
TCTGATGTCTAAATCTGCGT+CGG + chr5_1:53073009-53073028 None:intergenic 40.0%
CTTGTATCTGTGTCAGATAG+TGG - chr5_1:53073090-53073109 Msa0736540:CDS 40.0%
!! TTGAAGTGTCGATGCTTCAT+AGG - chr5_1:53073126-53073145 Msa0736540:CDS 40.0%
CGATGCTTCATAGGAATGTA+TGG - chr5_1:53073135-53073154 Msa0736540:intron 40.0%
!! CTTTGTTGGTTTCTTGAACC+AGG - chr5_1:53073196-53073215 Msa0736540:intron 40.0%
AGATCTTCATCTGGACTCAT+TGG - chr5_1:53073280-53073299 Msa0736540:intron 40.0%
! CATCTGGACTCATTGGTTAT+TGG - chr5_1:53073287-53073306 Msa0736540:intron 40.0%
! ACTCATTGGTTATTGGAAGC+AGG - chr5_1:53073294-53073313 Msa0736540:intron 40.0%
! CTCATTGGTTATTGGAAGCA+GGG - chr5_1:53073295-53073314 Msa0736540:intron 40.0%
! TCATTGGTTATTGGAAGCAG+GGG - chr5_1:53073296-53073315 Msa0736540:intron 40.0%
! GGGGTTTAGGTTCCATTAAA+AGG - chr5_1:53073315-53073334 Msa0736540:intron 40.0%
ATTCCCTTGACTACAGTAAC+CGG + chr5_1:53073501-53073520 None:intergenic 40.0%
AATCAATGCCTAGACTTGGA+AGG + chr5_1:53073528-53073547 None:intergenic 40.0%
! GCTAGCCAAAGCATTTCAAA+AGG + chr5_1:53073621-53073640 None:intergenic 40.0%
! TGCTTTTGCCATCTTTTCCT+AGG + chr5_1:53072165-53072184 None:intergenic 40.0%
! ATGGCAAAAGCACACATAGT+TGG - chr5_1:53072173-53072192 Msa0736540:three_prime_UTR 40.0%
ACACATAGTTGGAGTAGCTA+TGG - chr5_1:53072184-53072203 Msa0736540:three_prime_UTR 40.0%
! TTTCTAGTGTGATCAGCTGA+GGG + chr5_1:53072302-53072321 None:intergenic 40.0%
!! AAGGAGCTTTTTGAAGACAC+TGG - chr5_1:53072320-53072339 Msa0736540:three_prime_UTR 40.0%
TGTCTCTACGACCATAATTG+AGG - chr5_1:53072501-53072520 Msa0736540:CDS 40.0%
AAATCGACTATAAGTGACCG+TGG - chr5_1:53072526-53072545 Msa0736540:intron 40.0%
!! TGGTTGATGATTTGCAGCTT+TGG - chr5_1:53072801-53072820 Msa0736540:intron 40.0%
ATCTCCCTTAATTCCTCTAG+AGG + chr5_1:53072829-53072848 None:intergenic 40.0%
GAAACTGCCTCAAAAACCAA+AGG - chr5_1:53072904-53072923 Msa0736540:intron 40.0%
AACTGCCTCAAAAACCAAAG+GGG - chr5_1:53072906-53072925 Msa0736540:intron 40.0%
TCTGATGTCTAAATCTGCGT+CGG + chr5_1:53073009-53073028 None:intergenic 40.0%
CTTGTATCTGTGTCAGATAG+TGG - chr5_1:53073090-53073109 Msa0736540:CDS 40.0%
!! TTGAAGTGTCGATGCTTCAT+AGG - chr5_1:53073126-53073145 Msa0736540:CDS 40.0%
CGATGCTTCATAGGAATGTA+TGG - chr5_1:53073135-53073154 Msa0736540:intron 40.0%
!! CTTTGTTGGTTTCTTGAACC+AGG - chr5_1:53073196-53073215 Msa0736540:intron 40.0%
AGATCTTCATCTGGACTCAT+TGG - chr5_1:53073280-53073299 Msa0736540:intron 40.0%
! CATCTGGACTCATTGGTTAT+TGG - chr5_1:53073287-53073306 Msa0736540:intron 40.0%
! ACTCATTGGTTATTGGAAGC+AGG - chr5_1:53073294-53073313 Msa0736540:intron 40.0%
! CTCATTGGTTATTGGAAGCA+GGG - chr5_1:53073295-53073314 Msa0736540:intron 40.0%
! TCATTGGTTATTGGAAGCAG+GGG - chr5_1:53073296-53073315 Msa0736540:intron 40.0%
! GGGGTTTAGGTTCCATTAAA+AGG - chr5_1:53073315-53073334 Msa0736540:intron 40.0%
ATTCCCTTGACTACAGTAAC+CGG + chr5_1:53073501-53073520 None:intergenic 40.0%
AATCAATGCCTAGACTTGGA+AGG + chr5_1:53073528-53073547 None:intergenic 40.0%
! GCTAGCCAAAGCATTTCAAA+AGG + chr5_1:53073621-53073640 None:intergenic 40.0%
ATTGTGCCTCGGAATTTCAG+TGG + chr5_1:53072139-53072158 None:intergenic 45.0%
!! TTCCTAGGAGTATTGTGCCT+CGG + chr5_1:53072150-53072169 None:intergenic 45.0%
! CTTTCTAGTGTGATCAGCTG+AGG + chr5_1:53072303-53072322 None:intergenic 45.0%
CTCAGCTGATCACACTAGAA+AGG - chr5_1:53072301-53072320 Msa0736540:three_prime_UTR 45.0%
ACATCGAAACCCTGATGTTG+CGG + chr5_1:53072484-53072503 None:intergenic 45.0%
! TGGTGCCTCTAGAGGAATTA+AGG - chr5_1:53072821-53072840 Msa0736540:intron 45.0%
! GGTGCCTCTAGAGGAATTAA+GGG - chr5_1:53072822-53072841 Msa0736540:intron 45.0%
TGGTTTCTTGAACCAGGCAA+TGG - chr5_1:53073202-53073221 Msa0736540:intron 45.0%
!! TGGTAGTGGCAAAGGTGTAT+TGG - chr5_1:53073222-53073241 Msa0736540:intron 45.0%
! GGTAGTGGCAAAGGTGTATT+GGG - chr5_1:53073223-53073242 Msa0736540:intron 45.0%
! GTAGTGGCAAAGGTGTATTG+GGG - chr5_1:53073224-53073243 Msa0736540:intron 45.0%
GCATTGCACAATTTCTCCCT+TGG + chr5_1:53073254-53073273 None:intergenic 45.0%
TGCTGTGCAAGATCTTCATC+TGG - chr5_1:53073271-53073290 Msa0736540:intron 45.0%
GTTATTGGAAGCAGGGGTTT+AGG - chr5_1:53073302-53073321 Msa0736540:intron 45.0%
TTCCCTTGACTACAGTAACC+GGG + chr5_1:53073500-53073519 None:intergenic 45.0%
GAATGCAACCTTCCAAGTCT+AGG - chr5_1:53073517-53073536 Msa0736540:intron 45.0%
ATTGTGCCTCGGAATTTCAG+TGG + chr5_1:53072139-53072158 None:intergenic 45.0%
!! TTCCTAGGAGTATTGTGCCT+CGG + chr5_1:53072150-53072169 None:intergenic 45.0%
! CTTTCTAGTGTGATCAGCTG+AGG + chr5_1:53072303-53072322 None:intergenic 45.0%
CTCAGCTGATCACACTAGAA+AGG - chr5_1:53072301-53072320 Msa0736540:three_prime_UTR 45.0%
ACATCGAAACCCTGATGTTG+CGG + chr5_1:53072484-53072503 None:intergenic 45.0%
! TGGTGCCTCTAGAGGAATTA+AGG - chr5_1:53072821-53072840 Msa0736540:intron 45.0%
! GGTGCCTCTAGAGGAATTAA+GGG - chr5_1:53072822-53072841 Msa0736540:intron 45.0%
TGGTTTCTTGAACCAGGCAA+TGG - chr5_1:53073202-53073221 Msa0736540:intron 45.0%
!! TGGTAGTGGCAAAGGTGTAT+TGG - chr5_1:53073222-53073241 Msa0736540:intron 45.0%
! GGTAGTGGCAAAGGTGTATT+GGG - chr5_1:53073223-53073242 Msa0736540:intron 45.0%
! GTAGTGGCAAAGGTGTATTG+GGG - chr5_1:53073224-53073243 Msa0736540:intron 45.0%
GCATTGCACAATTTCTCCCT+TGG + chr5_1:53073254-53073273 None:intergenic 45.0%
TGCTGTGCAAGATCTTCATC+TGG - chr5_1:53073271-53073290 Msa0736540:intron 45.0%
GTTATTGGAAGCAGGGGTTT+AGG - chr5_1:53073302-53073321 Msa0736540:intron 45.0%
TTCCCTTGACTACAGTAACC+GGG + chr5_1:53073500-53073519 None:intergenic 45.0%
GAATGCAACCTTCCAAGTCT+AGG - chr5_1:53073517-53073536 Msa0736540:intron 45.0%
TTCCGAGGCACAATACTCCT+AGG - chr5_1:53072145-53072164 Msa0736540:three_prime_UTR 50.0%
TATACGACTGTGGTCTGGAC+CGG - chr5_1:53072457-53072476 Msa0736540:CDS 50.0%
CTTGAACCAGGCAATGGTAG+TGG - chr5_1:53073208-53073227 Msa0736540:intron 50.0%
AGGTGTATTGGGGAGATCCA+AGG - chr5_1:53073234-53073253 Msa0736540:intron 50.0%
GGTGTATTGGGGAGATCCAA+GGG - chr5_1:53073235-53073254 Msa0736540:intron 50.0%
AGGCAGTGTTAGCAAGCATG+TGG - chr5_1:53073458-53073477 Msa0736540:intron 50.0%
GGTGACAAATGCTTCTTGCC+CGG - chr5_1:53073479-53073498 Msa0736540:intron 50.0%
TTGCCCGGTTACTGTAGTCA+AGG - chr5_1:53073494-53073513 Msa0736540:intron 50.0%
TGCCCGGTTACTGTAGTCAA+GGG - chr5_1:53073495-53073514 Msa0736540:intron 50.0%
TTCCGAGGCACAATACTCCT+AGG - chr5_1:53072145-53072164 Msa0736540:three_prime_UTR 50.0%
TATACGACTGTGGTCTGGAC+CGG - chr5_1:53072457-53072476 Msa0736540:CDS 50.0%
CTTGAACCAGGCAATGGTAG+TGG - chr5_1:53073208-53073227 Msa0736540:intron 50.0%
AGGTGTATTGGGGAGATCCA+AGG - chr5_1:53073234-53073253 Msa0736540:intron 50.0%
GGTGTATTGGGGAGATCCAA+GGG - chr5_1:53073235-53073254 Msa0736540:intron 50.0%
AGGCAGTGTTAGCAAGCATG+TGG - chr5_1:53073458-53073477 Msa0736540:intron 50.0%
GGTGACAAATGCTTCTTGCC+CGG - chr5_1:53073479-53073498 Msa0736540:intron 50.0%
TTGCCCGGTTACTGTAGTCA+AGG - chr5_1:53073494-53073513 Msa0736540:intron 50.0%
TGCCCGGTTACTGTAGTCAA+GGG - chr5_1:53073495-53073514 Msa0736540:intron 50.0%
CTAGTGTGATCAGCTGAGGG+AGG + chr5_1:53072299-53072318 None:intergenic 55.0%
ATACGACTGTGGTCTGGACC+GGG - chr5_1:53072458-53072477 Msa0736540:CDS 55.0%
CGCAGCCCAGATTGCATTCA+TGG - chr5_1:53072674-53072693 Msa0736540:CDS 55.0%
!! TGCAGCTTTGGTGCCTCTAG+AGG - chr5_1:53072813-53072832 Msa0736540:intron 55.0%
CCTTTGCCACTACCATTGCC+TGG + chr5_1:53073217-53073236 None:intergenic 55.0%
!! CCAGGCAATGGTAGTGGCAA+AGG - chr5_1:53073214-53073233 Msa0736540:intron 55.0%
CTAGTGTGATCAGCTGAGGG+AGG + chr5_1:53072299-53072318 None:intergenic 55.0%
ATACGACTGTGGTCTGGACC+GGG - chr5_1:53072458-53072477 Msa0736540:CDS 55.0%
CGCAGCCCAGATTGCATTCA+TGG - chr5_1:53072674-53072693 Msa0736540:CDS 55.0%
!! TGCAGCTTTGGTGCCTCTAG+AGG - chr5_1:53072813-53072832 Msa0736540:intron 55.0%
CCTTTGCCACTACCATTGCC+TGG + chr5_1:53073217-53073236 None:intergenic 55.0%
!! CCAGGCAATGGTAGTGGCAA+AGG - chr5_1:53073214-53073233 Msa0736540:intron 55.0%
TGTGATCAGCTGAGGGAGGC+TGG + chr5_1:53072295-53072314 None:intergenic 60.0%
GAAACCCTGATGTTGCGGCC+CGG + chr5_1:53072479-53072498 None:intergenic 60.0%
TGTGATCAGCTGAGGGAGGC+TGG + chr5_1:53072295-53072314 None:intergenic 60.0%
GAAACCCTGATGTTGCGGCC+CGG + chr5_1:53072479-53072498 None:intergenic 60.0%
TGGACCGGGCCGCAACATCA+GGG - chr5_1:53072472-53072491 Msa0736540:CDS 65.0%
TGGACCGGGCCGCAACATCA+GGG - chr5_1:53072472-53072491 Msa0736540:CDS 65.0%
CTGGACCGGGCCGCAACATC+AGG - chr5_1:53072471-53072490 Msa0736540:CDS 70.0%
CTGGACCGGGCCGCAACATC+AGG - chr5_1:53072471-53072490 Msa0736540:CDS 70.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 53072131 53073838 53072131 ID=Msa0736540;Name=Msa0736540
chr5_1 mRNA 53072131 53073838 53072131 ID=Msa0736540-mRNA-1;Parent=Msa0736540;Name=Msa0736540-mRNA-1;_AED=0.09;_eAED=0.09;_QI=42|1|1|1|1|1|4|293|166
chr5_1 exon 53072131 53072520 53072131 ID=Msa0736540-mRNA-1:exon:14933;Parent=Msa0736540-mRNA-1
chr5_1 exon 53072633 53072751 53072633 ID=Msa0736540-mRNA-1:exon:14932;Parent=Msa0736540-mRNA-1
chr5_1 exon 53073042 53073151 53073042 ID=Msa0736540-mRNA-1:exon:14931;Parent=Msa0736540-mRNA-1
chr5_1 exon 53073622 53073838 53073622 ID=Msa0736540-mRNA-1:exon:14930;Parent=Msa0736540-mRNA-1
chr5_1 five_prime_UTR 53073797 53073838 53073797 ID=Msa0736540-mRNA-1:five_prime_utr;Parent=Msa0736540-mRNA-1
chr5_1 CDS 53073622 53073796 53073622 ID=Msa0736540-mRNA-1:cds;Parent=Msa0736540-mRNA-1
chr5_1 CDS 53073042 53073151 53073042 ID=Msa0736540-mRNA-1:cds;Parent=Msa0736540-mRNA-1
chr5_1 CDS 53072633 53072751 53072633 ID=Msa0736540-mRNA-1:cds;Parent=Msa0736540-mRNA-1
chr5_1 CDS 53072424 53072520 53072424 ID=Msa0736540-mRNA-1:cds;Parent=Msa0736540-mRNA-1
chr5_1 three_prime_UTR 53072131 53072423 53072131 ID=Msa0736540-mRNA-1:three_prime_utr;Parent=Msa0736540-mRNA-1
Gene Sequence

>Msa0736540

ATGGCAAAAGCACACATAGTTGGAGTAGCTATGGACTTCTCTCCTACCAGTAAATTAGCACTTAGATGGGCTGTTGATAATCTGATCAACAAAAATGATCAAATCATCATGATCAATGTCCAGCCTCCCTCAGCTGATCACACTAGAAAGGAGCTTTTTGAAGACACTGGATCACCTTTGGTGCCTCTAGAGGAATTAAGGGAGATAAACTTTACAAAGCAATATGGAATTGCTAAGGATCCTGAAGTCATAGATATTCTTGAAACTGCCTCAAAAACCAAAGGGGCAATGGTAGTGGCAAAGGTGTATTGGGGAGATCCAAGGGAGAAATTGTGCAATGCTGTGCAAGATCTTCATCTGGACTCATTGGTTATTGGAAGCAGGGGTTTAGGTTCCATTAAAAGTGTGTTGTTAGGCAGTGTTAGCAAGCATGTGGTGACAAATGCTTCTTGCCCGGTTACTGTAGTCAAGGGAATGCAACCTTCCAAGTCTAGGCATTGA
Protein sequence

>Msa0736540

MAKAHIVGVAMDFSPTSKLALRWAVDNLINKNDQIIMINVQPPSADHTRKELFEDTGSPLVPLEELREINFTKQYGIAKDPEVIDILETASKTKGAMVVAKVYWGDPREKLCNAVQDLHLDSLVIGSRGLGSIKSVLLGSVSKHVVTNASCPVTVVKGMQPSKSRH*