AlfalfaGEDB | Msa0812030

M. sativa cultivar zhongmu-4 / Msa0812030

KEGG: -
GO: GO:0000166 GO:0003674 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0008144 GO:0016020 GO:0016208 GO:0017076 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0036094 GO:0043167 GO:0043168 GO:0043169 GO:0044464 GO:0071944 GO:0097159 GO:0097367 GO:1901265 GO:1901363
PFAM: PF00582.29

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0812030	XP_003616191.1	96.386	166	6	0	1	166	1	166	1.54e-113	329

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0812030	sp\|Q8LGG8\|USPAL_ARATH	29.252	147	93	3	14	157	24	162	2.36e-14	70.1

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0812030	G7KFW2	96.386	166	6	0	1	166	1	166	7.34e-114	329

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0736540	Msa0812030	0.938980	2.737628e-99	-8.615850e-47
Msa0781680	Msa0812030	0.915804	3.807438e-85	-8.615850e-47
Msa0812030	Msa0856060	0.931302	4.619201e-94	-8.615850e-47
Msa0812030	Msa0993190	0.802319	5.934859e-49	-8.615850e-47
Msa0812030	Msa1289050	0.817504	3.187034e-52	-8.615850e-47
Msa0812030	Msa1289090	0.818208	2.210325e-52	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0812030	MtrunA17_Chr5g0433951	96.386	166	6	0	1	166	1	166	1.41e-117	329
Msa0812030	MtrunA17_Chr4g0008801	56.051	157	69	0	1	157	1	157	1.41e-59	182
Msa0812030	MtrunA17_Chr4g0032261	54.777	157	71	0	1	157	1	157	9.86e-59	180
Msa0812030	MtrunA17_Chr7g0240701	49.045	157	80	0	1	157	1	157	6.55e-54	168
Msa0812030	MtrunA17_Chr2g0294421	50.318	157	78	0	1	157	1	157	6.56e-51	160
Msa0812030	MtrunA17_Chr1g0194801	35.849	159	86	6	9	161	10	158	3.59e-20	82.0
Msa0812030	MtrunA17_Chr5g0427291	37.349	166	88	5	6	158	9	171	9.89e-19	78.6
Msa0812030	MtrunA17_Chr1g0192651	35.714	154	83	6	9	157	13	155	1.44e-18	77.8
Msa0812030	MtrunA17_Chr4g0001641	54.098	61	28	0	98	158	6	66	1.35e-17	72.8
Msa0812030	MtrunA17_Chr1g0176311	27.933	179	80	5	7	157	24	181	1.71e-16	73.2

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0812030	AT3G03270.2	70.701	157	46	0	1	157	1	157	5.11e-79	232
Msa0812030	AT3G03270.1	68.657	134	42	0	1	134	1	134	5.17e-63	192
Msa0812030	AT3G53990.1	50.318	157	78	0	1	157	1	157	6.12e-54	168
Msa0812030	AT3G17020.1	46.405	153	81	1	7	158	9	161	8.48e-49	155
Msa0812030	AT3G53990.2	42.857	98	56	0	1	98	1	98	4.07e-24	91.7
Msa0812030	AT1G09740.2	37.736	159	87	4	7	157	10	164	4.65e-19	79.7
Msa0812030	AT1G09740.1	37.736	159	87	4	7	157	10	164	4.65e-19	79.7
Msa0812030	AT3G62550.1	34.356	163	88	5	3	157	5	156	6.04e-18	76.6
Msa0812030	AT3G58450.2	32.317	164	84	6	7	157	32	181	2.03e-17	75.9
Msa0812030	AT3G17020.2	35.106	94	60	1	7	99	9	102	7.86e-17	72.4

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Find 56 sgRNAs with CRISPR-Local

Find 202 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACAACATAAATACCCCTT+TGG	0.402357	5_3:+41853923	None:intergenic
AAAGCAATATGGAATTGCTA+AGG	0.573242	5_3:-41853985	Msa0812030:CDS
AAGAAACCACTGAAATTCCG+AGG	0.659277	5_3:-41854713	Msa0812030:five_prime_UTR
AATCAATGCCTAAACTTGGA+AGG	0.563474	5_3:+41853319	None:intergenic
ACAATACTCCTAGGAAAAGA+TGG	0.525752	5_3:-41854689	Msa0812030:exon
ACACATAGTTGGAGTAGCTA+TGG	0.571440	5_3:-41854659	Msa0812030:CDS
ACTCAGATAAATTGTAATGA+TGG	0.464485	5_3:+41853142	None:intergenic
ACTCATTGGTTATTGGAAGC+AGG	0.470238	5_3:-41853550	Msa0812030:CDS
AGATCTTCATCTGGACTCAT+TGG	0.398485	5_3:-41853564	Msa0812030:CDS
AGGCAGTGTTAGCAAGCATG+TGG	0.730937	5_3:-41853386	Msa0812030:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CAGTAAATTAGCACTTAGAT+GGG	-	chr5_3:41853117-41853136	Msa0812030:three_prime_UTR	30.0%
TGCTGAAACAATCTCATTTA+TGG	-	chr5_3:41853512-41853531	Msa0812030:intron	30.0%
AAAATCCATGAATGCAATCT+GGG	+	chr5_3:41853579-41853598	None:intergenic	30.0%
AAAAATCCATGAATGCAATC+TGG	+	chr5_3:41853580-41853599	None:intergenic	30.0%
GCACATACTATATGTTGTAT+AGG	-	chr5_3:41853618-41853637	Msa0812030:CDS	30.0%
AAAGCAATATGGAATTGCTA+AGG	-	chr5_3:41853755-41853774	Msa0812030:intron	30.0%
TCAAGAATATCTATGACTTC+AGG	+	chr5_3:41853783-41853802	None:intergenic	30.0%
AAACAACATAAATACCCCTT+TGG	+	chr5_3:41853820-41853839	None:intergenic	30.0%
TGGTGTAGTTAACACATATA+TGG	-	chr5_3:41854051-41854070	Msa0812030:intron	30.0%
ATCAAATCAATGCCTAAACT+TGG	+	chr5_3:41854428-41854447	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_3	gene	41853028	41854734	41853028	ID=Msa0812030;Name=Msa0812030
chr5_3	mRNA	41853028	41854734	41853028	ID=Msa0812030-mRNA-1;Parent=Msa0812030;Name=Msa0812030-mRNA-1;_AED=0.09;_eAED=0.09;_QI=42\|1\|1\|1\|1\|1\|4\|293\|166
chr5_3	exon	41853028	41853417	41853028	ID=Msa0812030-mRNA-1:exon:12844;Parent=Msa0812030-mRNA-1
chr5_3	exon	41853530	41853648	41853530	ID=Msa0812030-mRNA-1:exon:12843;Parent=Msa0812030-mRNA-1
chr5_3	exon	41853938	41854047	41853938	ID=Msa0812030-mRNA-1:exon:12842;Parent=Msa0812030-mRNA-1
chr5_3	exon	41854518	41854734	41854518	ID=Msa0812030-mRNA-1:exon:12841;Parent=Msa0812030-mRNA-1
chr5_3	five_prime_UTR	41854693	41854734	41854693	ID=Msa0812030-mRNA-1:five_prime_utr;Parent=Msa0812030-mRNA-1
chr5_3	CDS	41854518	41854692	41854518	ID=Msa0812030-mRNA-1:cds;Parent=Msa0812030-mRNA-1
chr5_3	CDS	41853938	41854047	41853938	ID=Msa0812030-mRNA-1:cds;Parent=Msa0812030-mRNA-1
chr5_3	CDS	41853530	41853648	41853530	ID=Msa0812030-mRNA-1:cds;Parent=Msa0812030-mRNA-1
chr5_3	CDS	41853321	41853417	41853321	ID=Msa0812030-mRNA-1:cds;Parent=Msa0812030-mRNA-1
chr5_3	three_prime_UTR	41853028	41853320	41853028	ID=Msa0812030-mRNA-1:three_prime_utr;Parent=Msa0812030-mRNA-1

Gene Sequence

>Msa0812030

ATGGCAAAAGCACACATAGTTGGAGTAGCTATGGACTTCTCTCCTACCAGTAAATTAGCACTTAGATGGGCTGTTGATAATCTGATCAACAAAAATGATCAAATCATCATGATCAATGTTCAGCCTCCCTCAGCTGATCACACTAGAAAGGAGCTTTTTGAAGACACTGGATCACCTTTGGTGCCTCTAGAGGAATTAAGGGAGATAAACTTTACAAAGCAATATGGAATTGCTAAGGATCCTGAAGTCATAGATATTCTTGAAACTGCCTCAAAAACCAAAGGGGCAATGGTAGTGGCAAAGGTGTATTGGGGAGATCCAAGGGAGAAATTGTGCAATGCTGTGCAAGATCTTCATCTGGACTCATTGGTTATTGGAAGCAGGGGTTTAGGTTCCATTAAAAGTGTGTTGTTAGGCAGTGTTAGCAAGCATGTGGTGACAAATGCTTCTTGCCCGGTTACTGTAGTCAAGGGAATGCAACCTTCCAAGTTTAGGCATTGA

Protein sequence

>Msa0812030

MAKAHIVGVAMDFSPTSKLALRWAVDNLINKNDQIIMINVQPPSADHTRKELFEDTGSPLVPLEELREINFTKQYGIAKDPEVIDILETASKTKGAMVVAKVYWGDPREKLCNAVQDLHLDSLVIGSRGLGSIKSVLLGSVSKHVVTNASCPVTVVKGMQPSKFRH*