AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0812030

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0812030 MtrunA17_Chr5g0433951 96.386 166 6 0 1 166 1 166 1.41e-117 329
Msa0812030 MtrunA17_Chr4g0008801 56.051 157 69 0 1 157 1 157 1.41e-59 182
Msa0812030 MtrunA17_Chr4g0032261 54.777 157 71 0 1 157 1 157 9.86e-59 180
Msa0812030 MtrunA17_Chr7g0240701 49.045 157 80 0 1 157 1 157 6.55e-54 168
Msa0812030 MtrunA17_Chr2g0294421 50.318 157 78 0 1 157 1 157 6.56e-51 160
Msa0812030 MtrunA17_Chr1g0194801 35.849 159 86 6 9 161 10 158 3.59e-20 82.0
Msa0812030 MtrunA17_Chr5g0427291 37.349 166 88 5 6 158 9 171 9.89e-19 78.6
Msa0812030 MtrunA17_Chr1g0192651 35.714 154 83 6 9 157 13 155 1.44e-18 77.8
Msa0812030 MtrunA17_Chr4g0001641 54.098 61 28 0 98 158 6 66 1.35e-17 72.8
Msa0812030 MtrunA17_Chr1g0176311 27.933 179 80 5 7 157 24 181 1.71e-16 73.2
Msa0812030 MtrunA17_Chr1g0176321 28.333 180 84 4 7 157 5 168 1.88e-15 70.1
Msa0812030 MtrunA17_Chr6g0450171 29.932 147 92 3 14 157 24 162 1.90e-14 67.4
Msa0812030 MtrunA17_Chr2g0323291 30.435 161 96 4 7 157 27 181 9.55e-14 66.2
Msa0812030 MtrunA17_Chr1g0195661 28.387 155 96 5 9 157 16 161 2.55e-12 61.6
Msa0812030 MtrunA17_Chr6g0464761 50.000 56 21 1 98 153 15 63 3.85e-12 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0812030 AT3G03270.2 70.701 157 46 0 1 157 1 157 5.11e-79 232
Msa0812030 AT3G03270.1 68.657 134 42 0 1 134 1 134 5.17e-63 192
Msa0812030 AT3G53990.1 50.318 157 78 0 1 157 1 157 6.12e-54 168
Msa0812030 AT3G17020.1 46.405 153 81 1 7 158 9 161 8.48e-49 155
Msa0812030 AT3G53990.2 42.857 98 56 0 1 98 1 98 4.07e-24 91.7
Msa0812030 AT1G09740.2 37.736 159 87 4 7 157 10 164 4.65e-19 79.7
Msa0812030 AT1G09740.1 37.736 159 87 4 7 157 10 164 4.65e-19 79.7
Msa0812030 AT3G62550.1 34.356 163 88 5 3 157 5 156 6.04e-18 76.6
Msa0812030 AT3G58450.2 32.317 164 84 6 7 157 32 181 2.03e-17 75.9
Msa0812030 AT3G17020.2 35.106 94 60 1 7 99 9 102 7.86e-17 72.4
Msa0812030 AT3G58450.1 32.544 169 84 6 7 157 32 188 1.61e-16 73.9
Msa0812030 AT1G11360.2 29.870 154 105 1 7 157 40 193 3.74e-16 73.6
Msa0812030 AT1G11360.1 29.870 154 105 1 7 157 40 193 3.74e-16 73.6
Msa0812030 AT1G11360.4 29.870 154 105 1 7 157 40 193 3.74e-16 73.6
Msa0812030 AT1G11360.3 29.870 154 105 1 7 157 40 193 3.74e-16 73.6
Msa0812030 AT1G68300.1 31.818 154 94 4 7 157 12 157 1.93e-15 70.1
Msa0812030 AT3G01520.1 29.252 147 93 3 14 157 24 162 2.40e-15 70.1
Msa0812030 AT5G14680.1 28.571 147 94 3 14 157 24 162 1.22e-14 68.2
Msa0812030 AT3G11930.4 29.560 159 101 2 9 157 37 194 1.22e-14 68.9
Msa0812030 AT3G11930.1 29.560 159 99 3 9 157 37 192 2.45e-14 67.8
Msa0812030 AT3G11930.2 29.560 159 100 3 9 157 37 193 5.27e-14 67.0
Msa0812030 AT5G54430.3 29.932 147 88 3 7 144 50 190 8.78e-11 58.9
Msa0812030 AT5G54430.6 29.932 147 88 3 7 144 50 190 8.78e-11 58.9
Msa0812030 AT5G54430.1 29.932 147 88 3 7 144 50 190 9.06e-11 58.9
Msa0812030 AT5G54430.4 29.932 147 88 3 7 144 50 190 9.06e-11 58.9
Msa0812030 AT5G54430.2 29.932 147 88 3 7 144 50 190 9.78e-11 58.9
Msa0812030 AT5G54430.5 29.932 147 88 3 7 144 50 190 9.78e-11 58.9

Find 56 sgRNAs with CRISPR-Local

Find 202 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGTGCCTCTAGAGGAATTA+AGG 0.233780 5_3:-41854022 Msa0812030:CDS
CATCTGGACTCATTGGTTAT+TGG 0.247760 5_3:-41853557 Msa0812030:CDS
GGTAGTGGCAAAGGTGTATT+GGG 0.310523 5_3:-41853621 Msa0812030:CDS
ATGATTATTAATATTTACTT+TGG 0.311087 5_3:-41853292 Msa0812030:three_prime_UTR
CTTCATTTCAGTGTGTTGTT+AGG 0.317283 5_3:-41853406 Msa0812030:intron
GTTATTGGAAGCAGGGGTTT+AGG 0.321337 5_3:-41853542 Msa0812030:CDS
CCATCTAAGTGCTAATTTAC+TGG 0.336238 5_3:+41854624 None:intergenic
CCAGTAAATTAGCACTTAGA+TGG 0.339331 5_3:-41854624 Msa0812030:CDS
GGGGTTTAGGTTCCATTAAA+AGG 0.346706 5_3:-41853529 Msa0812030:intron
TCAAGAATATCTATGACTTC+AGG 0.358570 5_3:+41853960 None:intergenic
GAATGCAACCTTCCAAGTTT+AGG 0.395882 5_3:-41853327 Msa0812030:CDS
AGATCTTCATCTGGACTCAT+TGG 0.398485 5_3:-41853564 Msa0812030:CDS
CATGAAACAATTGTCACATA+AGG 0.399976 5_3:-41853041 Msa0812030:three_prime_UTR
AAACAACATAAATACCCCTT+TGG 0.402357 5_3:+41853923 None:intergenic
ATAAACTTTACAAAGCAATA+TGG 0.410129 5_3:-41853996 Msa0812030:CDS
ATTCCCTTGACTACAGTAAC+CGG 0.414500 5_3:+41853346 None:intergenic
TGATTATTAATATTTACTTT+GGG 0.421374 5_3:-41853291 Msa0812030:three_prime_UTR
TGGTAGTGGCAAAGGTGTAT+TGG 0.429624 5_3:-41853622 Msa0812030:CDS
GCATTGCACAATTTCTCCCT+TGG 0.434268 5_3:+41853593 None:intergenic
GGTGCCTCTAGAGGAATTAA+GGG 0.447240 5_3:-41854021 Msa0812030:CDS
TGCTGTGCAAGATCTTCATC+TGG 0.451178 5_3:-41853573 Msa0812030:CDS
ACTCAGATAAATTGTAATGA+TGG 0.464485 5_3:+41853142 None:intergenic
ATGAAACAATTGTCACATAA+GGG 0.468956 5_3:-41853040 Msa0812030:three_prime_UTR
CTCATTGGTTATTGGAAGCA+GGG 0.470129 5_3:-41853549 Msa0812030:CDS
ACTCATTGGTTATTGGAAGC+AGG 0.470238 5_3:-41853550 Msa0812030:CDS
CTTGAACCAGGCAATGGTAG+TGG 0.472382 5_3:-41853636 Msa0812030:intron
ATCAAATCAATGCCTAAACT+TGG 0.476315 5_3:+41853315 None:intergenic
ATGGCAAAAGCACACATAGT+TGG 0.510567 5_3:-41854670 Msa0812030:CDS
TTCCTAGGAGTATTGTGCCT+CGG 0.514550 5_3:+41854696 None:intergenic
CTTTCTAGTGTGATCAGCTG+AGG 0.523922 5_3:+41854543 None:intergenic
CTCAGCTGATCACACTAGAA+AGG 0.525726 5_3:-41854542 Msa0812030:CDS
ACAATACTCCTAGGAAAAGA+TGG 0.525752 5_3:-41854689 Msa0812030:exon
GCTAGCCAAAGCATTTCAAA+AGG 0.531277 5_3:+41853226 None:intergenic
TGCAGCTTTGGTGCCTCTAG+AGG 0.535834 5_3:-41854030 Msa0812030:intron
CCAGGCAATGGTAGTGGCAA+AGG 0.540554 5_3:-41853630 Msa0812030:intron
ATCTCCCTTAATTCCTCTAG+AGG 0.547571 5_3:+41854017 None:intergenic
TTCCGAGGCACAATACTCCT+AGG 0.555541 5_3:-41854698 Msa0812030:five_prime_UTR
TTGCCCGGTTACTGTAGTCA+AGG 0.560158 5_3:-41853350 Msa0812030:CDS
AGGTGTATTGGGGAGATCCA+AGG 0.560692 5_3:-41853610 Msa0812030:CDS
AATCAATGCCTAAACTTGGA+AGG 0.563474 5_3:+41853319 None:intergenic
ACACATAGTTGGAGTAGCTA+TGG 0.571440 5_3:-41854659 Msa0812030:CDS
AAAGCAATATGGAATTGCTA+AGG 0.573242 5_3:-41853985 Msa0812030:CDS
TGCCCGGTTACTGTAGTCAA+GGG 0.580340 5_3:-41853349 Msa0812030:CDS
CTAAGTGCTAATTTACTGGT+AGG 0.583052 5_3:+41854628 None:intergenic
TTTCTAGTGTGATCAGCTGA+GGG 0.583250 5_3:+41854544 None:intergenic
GGTGACAAATGCTTCTTGCC+CGG 0.585729 5_3:-41853365 Msa0812030:CDS
CAGTAAATTAGCACTTAGAT+GGG 0.588277 5_3:-41854623 Msa0812030:CDS
GGTGTATTGGGGAGATCCAA+GGG 0.593103 5_3:-41853609 Msa0812030:CDS
ATTGTGCCTCGGAATTTCAG+TGG 0.603572 5_3:+41854707 None:intergenic
CTAGTGTGATCAGCTGAGGG+AGG 0.611787 5_3:+41854547 None:intergenic
TCATTGGTTATTGGAAGCAG+GGG 0.616498 5_3:-41853548 Msa0812030:CDS
TTCCCTTGACTACAGTAACC+GGG 0.638321 5_3:+41853347 None:intergenic
AAGAAACCACTGAAATTCCG+AGG 0.659277 5_3:-41854713 Msa0812030:five_prime_UTR
GTAGTGGCAAAGGTGTATTG+GGG 0.678633 5_3:-41853620 Msa0812030:CDS
ATGTGTCATATATTGATGAG+AGG 0.679189 5_3:-41853092 Msa0812030:three_prime_UTR
AGGCAGTGTTAGCAAGCATG+TGG 0.730937 5_3:-41853386 Msa0812030:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGATTATTAATATTTACTT+TGG - chr5_3:41854448-41854467 Msa0812030:intron 10.0%
!! TGATTATTAATATTTACTTT+GGG - chr5_3:41854449-41854468 Msa0812030:intron 10.0%
!! ATGATTATTAATATTTACTT+TGG - chr5_3:41854448-41854467 Msa0812030:intron 10.0%
!! TGATTATTAATATTTACTTT+GGG - chr5_3:41854449-41854468 Msa0812030:intron 10.0%
!!! TTTTTTTCTCATGTTATTAT+CGG + chr5_3:41853941-41853960 None:intergenic 15.0%
!!! TTTTTTTCTCATGTTATTAT+CGG + chr5_3:41853941-41853960 None:intergenic 15.0%
!! TATGATAATTCAAAGAATTG+CGG - chr5_3:41853324-41853343 Msa0812030:CDS 20.0%
!! ATAAACTTTACAAAGCAATA+TGG - chr5_3:41853744-41853763 Msa0812030:intron 20.0%
!!! TAAACTATAGTACCTTTTAA+TGG + chr5_3:41854226-41854245 None:intergenic 20.0%
!! TATGATAATTCAAAGAATTG+CGG - chr5_3:41853324-41853343 Msa0812030:CDS 20.0%
!! ATAAACTTTACAAAGCAATA+TGG - chr5_3:41853744-41853763 Msa0812030:intron 20.0%
!!! TAAACTATAGTACCTTTTAA+TGG + chr5_3:41854226-41854245 None:intergenic 20.0%
! TAAAAGAAAGAGAATGAAAG+AGG + chr5_3:41853255-41853274 None:intergenic 25.0%
!! ACTGATGTTATTTAAAAGCT+TGG + chr5_3:41853289-41853308 None:intergenic 25.0%
! TTAATAACTACAGAAAACCA+CGG + chr5_3:41853443-41853462 None:intergenic 25.0%
! TATGTTGTATAGGTTTATCT+TGG - chr5_3:41853628-41853647 Msa0812030:CDS 25.0%
!!! TGACAATTTTGTGTTTTGTT+TGG - chr5_3:41853678-41853697 Msa0812030:intron 25.0%
!!! TTTTTTATGTGTGTCTTTGT+TGG - chr5_3:41854078-41854097 Msa0812030:intron 25.0%
!! AGCTTGAATAATTGTCTTTT+TGG - chr5_3:41854534-41854553 Msa0812030:CDS 25.0%
! ACTCAGATAAATTGTAATGA+TGG + chr5_3:41854601-41854620 None:intergenic 25.0%
! ATGAAACAATTGTCACATAA+GGG - chr5_3:41854700-41854719 Msa0812030:five_prime_UTR 25.0%
! TAAAAGAAAGAGAATGAAAG+AGG + chr5_3:41853255-41853274 None:intergenic 25.0%
!! ACTGATGTTATTTAAAAGCT+TGG + chr5_3:41853289-41853308 None:intergenic 25.0%
! TTAATAACTACAGAAAACCA+CGG + chr5_3:41853443-41853462 None:intergenic 25.0%
! TATGTTGTATAGGTTTATCT+TGG - chr5_3:41853628-41853647 Msa0812030:CDS 25.0%
!!! TGACAATTTTGTGTTTTGTT+TGG - chr5_3:41853678-41853697 Msa0812030:intron 25.0%
!!! TTTTTTATGTGTGTCTTTGT+TGG - chr5_3:41854078-41854097 Msa0812030:intron 25.0%
!! AGCTTGAATAATTGTCTTTT+TGG - chr5_3:41854534-41854553 Msa0812030:CDS 25.0%
! ACTCAGATAAATTGTAATGA+TGG + chr5_3:41854601-41854620 None:intergenic 25.0%
! ATGAAACAATTGTCACATAA+GGG - chr5_3:41854700-41854719 Msa0812030:five_prime_UTR 25.0%
CAGTAAATTAGCACTTAGAT+GGG - chr5_3:41853117-41853136 Msa0812030:three_prime_UTR 30.0%
! AAGATTTTAAATAGCAGTCG+TGG + chr5_3:41853492-41853511 None:intergenic 30.0%
TGCTGAAACAATCTCATTTA+TGG - chr5_3:41853512-41853531 Msa0812030:intron 30.0%
! GATTTTTGATGTCTCAACAA+CGG + chr5_3:41853544-41853563 None:intergenic 30.0%
AAAATCCATGAATGCAATCT+GGG + chr5_3:41853579-41853598 None:intergenic 30.0%
AAAAATCCATGAATGCAATC+TGG + chr5_3:41853580-41853599 None:intergenic 30.0%
GCACATACTATATGTTGTAT+AGG - chr5_3:41853618-41853637 Msa0812030:CDS 30.0%
AAAGCAATATGGAATTGCTA+AGG - chr5_3:41853755-41853774 Msa0812030:intron 30.0%
TCAAGAATATCTATGACTTC+AGG + chr5_3:41853783-41853802 None:intergenic 30.0%
AAACAACATAAATACCCCTT+TGG + chr5_3:41853820-41853839 None:intergenic 30.0%
TGGTGTAGTTAACACATATA+TGG - chr5_3:41854051-41854070 Msa0812030:intron 30.0%
ATCAAATCAATGCCTAAACT+TGG + chr5_3:41854428-41854447 None:intergenic 30.0%
!! GAATAATTGTCTTTTTGGCA+TGG - chr5_3:41854539-41854558 Msa0812030:CDS 30.0%
ATGTGTCATATATTGATGAG+AGG - chr5_3:41854648-41854667 Msa0812030:CDS 30.0%
CATGAAACAATTGTCACATA+AGG - chr5_3:41854699-41854718 Msa0812030:five_prime_UTR 30.0%
CAGTAAATTAGCACTTAGAT+GGG - chr5_3:41853117-41853136 Msa0812030:three_prime_UTR 30.0%
! AAGATTTTAAATAGCAGTCG+TGG + chr5_3:41853492-41853511 None:intergenic 30.0%
TGCTGAAACAATCTCATTTA+TGG - chr5_3:41853512-41853531 Msa0812030:intron 30.0%
! GATTTTTGATGTCTCAACAA+CGG + chr5_3:41853544-41853563 None:intergenic 30.0%
AAAATCCATGAATGCAATCT+GGG + chr5_3:41853579-41853598 None:intergenic 30.0%
AAAAATCCATGAATGCAATC+TGG + chr5_3:41853580-41853599 None:intergenic 30.0%
GCACATACTATATGTTGTAT+AGG - chr5_3:41853618-41853637 Msa0812030:CDS 30.0%
AAAGCAATATGGAATTGCTA+AGG - chr5_3:41853755-41853774 Msa0812030:intron 30.0%
TCAAGAATATCTATGACTTC+AGG + chr5_3:41853783-41853802 None:intergenic 30.0%
AAACAACATAAATACCCCTT+TGG + chr5_3:41853820-41853839 None:intergenic 30.0%
TGGTGTAGTTAACACATATA+TGG - chr5_3:41854051-41854070 Msa0812030:intron 30.0%
ATCAAATCAATGCCTAAACT+TGG + chr5_3:41854428-41854447 None:intergenic 30.0%
!! GAATAATTGTCTTTTTGGCA+TGG - chr5_3:41854539-41854558 Msa0812030:CDS 30.0%
ATGTGTCATATATTGATGAG+AGG - chr5_3:41854648-41854667 Msa0812030:CDS 30.0%
CATGAAACAATTGTCACATA+AGG - chr5_3:41854699-41854718 Msa0812030:five_prime_UTR 30.0%
ACAATACTCCTAGGAAAAGA+TGG - chr5_3:41853051-41853070 Msa0812030:three_prime_UTR 35.0%
CTAAGTGCTAATTTACTGGT+AGG + chr5_3:41853115-41853134 None:intergenic 35.0%
CCATCTAAGTGCTAATTTAC+TGG + chr5_3:41853119-41853138 None:intergenic 35.0%
CCAGTAAATTAGCACTTAGA+TGG - chr5_3:41853116-41853135 Msa0812030:three_prime_UTR 35.0%
CGGTTAAATTTATACGACTG+TGG - chr5_3:41853344-41853363 Msa0812030:CDS 35.0%
AAATTTATACGACTGTGGTC+TGG - chr5_3:41853349-41853368 Msa0812030:CDS 35.0%
!! AGTCGATTTTGCCTCAATTA+TGG + chr5_3:41853412-41853431 None:intergenic 35.0%
CATATAGTATGTGCTAAGCA+AGG + chr5_3:41853612-41853631 None:intergenic 35.0%
AAACTGCCTCAAAAACCAAA+GGG - chr5_3:41853802-41853821 Msa0812030:intron 35.0%
!!! AAATACCCCTTTGGTTTTTG+AGG + chr5_3:41853811-41853830 None:intergenic 35.0%
! CTTCATTTCAGTGTGTTGTT+AGG - chr5_3:41854334-41854353 Msa0812030:intron 35.0%
AATCAATGCCTAAACTTGGA+AGG + chr5_3:41854424-41854443 None:intergenic 35.0%
!!! CTGTACCTTTTGAAATGCTT+TGG - chr5_3:41854509-41854528 Msa0812030:intron 35.0%
ACAATACTCCTAGGAAAAGA+TGG - chr5_3:41853051-41853070 Msa0812030:three_prime_UTR 35.0%
CTAAGTGCTAATTTACTGGT+AGG + chr5_3:41853115-41853134 None:intergenic 35.0%
CCATCTAAGTGCTAATTTAC+TGG + chr5_3:41853119-41853138 None:intergenic 35.0%
CCAGTAAATTAGCACTTAGA+TGG - chr5_3:41853116-41853135 Msa0812030:three_prime_UTR 35.0%
CGGTTAAATTTATACGACTG+TGG - chr5_3:41853344-41853363 Msa0812030:CDS 35.0%
AAATTTATACGACTGTGGTC+TGG - chr5_3:41853349-41853368 Msa0812030:CDS 35.0%
!! AGTCGATTTTGCCTCAATTA+TGG + chr5_3:41853412-41853431 None:intergenic 35.0%
CATATAGTATGTGCTAAGCA+AGG + chr5_3:41853612-41853631 None:intergenic 35.0%
AAACTGCCTCAAAAACCAAA+GGG - chr5_3:41853802-41853821 Msa0812030:intron 35.0%
!!! AAATACCCCTTTGGTTTTTG+AGG + chr5_3:41853811-41853830 None:intergenic 35.0%
! CTTCATTTCAGTGTGTTGTT+AGG - chr5_3:41854334-41854353 Msa0812030:intron 35.0%
AATCAATGCCTAAACTTGGA+AGG + chr5_3:41854424-41854443 None:intergenic 35.0%
!!! CTGTACCTTTTGAAATGCTT+TGG - chr5_3:41854509-41854528 Msa0812030:intron 35.0%
! TGCTTTTGCCATCTTTTCCT+AGG + chr5_3:41853062-41853081 None:intergenic 40.0%
! ATGGCAAAAGCACACATAGT+TGG - chr5_3:41853070-41853089 Msa0812030:three_prime_UTR 40.0%
ACACATAGTTGGAGTAGCTA+TGG - chr5_3:41853081-41853100 Msa0812030:three_prime_UTR 40.0%
! TTTCTAGTGTGATCAGCTGA+GGG + chr5_3:41853199-41853218 None:intergenic 40.0%
!! AAGGAGCTTTTTGAAGACAC+TGG - chr5_3:41853217-41853236 Msa0812030:three_prime_UTR 40.0%
TGTCTCTACGACCATAATTG+AGG - chr5_3:41853398-41853417 Msa0812030:CDS 40.0%
AAATCGACTATAAGTGACCG+TGG - chr5_3:41853423-41853442 Msa0812030:intron 40.0%
!! TGGTTGATGATTTGCAGCTT+TGG - chr5_3:41853698-41853717 Msa0812030:intron 40.0%
ATCTCCCTTAATTCCTCTAG+AGG + chr5_3:41853726-41853745 None:intergenic 40.0%
GAAACTGCCTCAAAAACCAA+AGG - chr5_3:41853801-41853820 Msa0812030:intron 40.0%
AACTGCCTCAAAAACCAAAG+GGG - chr5_3:41853803-41853822 Msa0812030:intron 40.0%
TCTGATGTCTAAATCTGCGT+CGG + chr5_3:41853905-41853924 None:intergenic 40.0%
CTTGTATCTGTGTCAGATAG+TGG - chr5_3:41853986-41854005 Msa0812030:CDS 40.0%
!! TTGAAGTGTCGATGCTTCAT+AGG - chr5_3:41854022-41854041 Msa0812030:CDS 40.0%
CGATGCTTCATAGGAATGTA+TGG - chr5_3:41854031-41854050 Msa0812030:intron 40.0%
!! CTTTGTTGGTTTCTTGAACC+AGG - chr5_3:41854092-41854111 Msa0812030:intron 40.0%
AGATCTTCATCTGGACTCAT+TGG - chr5_3:41854176-41854195 Msa0812030:intron 40.0%
! CATCTGGACTCATTGGTTAT+TGG - chr5_3:41854183-41854202 Msa0812030:intron 40.0%
! ACTCATTGGTTATTGGAAGC+AGG - chr5_3:41854190-41854209 Msa0812030:intron 40.0%
! CTCATTGGTTATTGGAAGCA+GGG - chr5_3:41854191-41854210 Msa0812030:intron 40.0%
! TCATTGGTTATTGGAAGCAG+GGG - chr5_3:41854192-41854211 Msa0812030:intron 40.0%
! GGGGTTTAGGTTCCATTAAA+AGG - chr5_3:41854211-41854230 Msa0812030:intron 40.0%
ATTCCCTTGACTACAGTAAC+CGG + chr5_3:41854397-41854416 None:intergenic 40.0%
GAATGCAACCTTCCAAGTTT+AGG - chr5_3:41854413-41854432 Msa0812030:intron 40.0%
! GCTAGCCAAAGCATTTCAAA+AGG + chr5_3:41854517-41854536 None:intergenic 40.0%
! TGCTTTTGCCATCTTTTCCT+AGG + chr5_3:41853062-41853081 None:intergenic 40.0%
! ATGGCAAAAGCACACATAGT+TGG - chr5_3:41853070-41853089 Msa0812030:three_prime_UTR 40.0%
ACACATAGTTGGAGTAGCTA+TGG - chr5_3:41853081-41853100 Msa0812030:three_prime_UTR 40.0%
! TTTCTAGTGTGATCAGCTGA+GGG + chr5_3:41853199-41853218 None:intergenic 40.0%
!! AAGGAGCTTTTTGAAGACAC+TGG - chr5_3:41853217-41853236 Msa0812030:three_prime_UTR 40.0%
TGTCTCTACGACCATAATTG+AGG - chr5_3:41853398-41853417 Msa0812030:CDS 40.0%
AAATCGACTATAAGTGACCG+TGG - chr5_3:41853423-41853442 Msa0812030:intron 40.0%
!! TGGTTGATGATTTGCAGCTT+TGG - chr5_3:41853698-41853717 Msa0812030:intron 40.0%
ATCTCCCTTAATTCCTCTAG+AGG + chr5_3:41853726-41853745 None:intergenic 40.0%
GAAACTGCCTCAAAAACCAA+AGG - chr5_3:41853801-41853820 Msa0812030:intron 40.0%
AACTGCCTCAAAAACCAAAG+GGG - chr5_3:41853803-41853822 Msa0812030:intron 40.0%
TCTGATGTCTAAATCTGCGT+CGG + chr5_3:41853905-41853924 None:intergenic 40.0%
CTTGTATCTGTGTCAGATAG+TGG - chr5_3:41853986-41854005 Msa0812030:CDS 40.0%
!! TTGAAGTGTCGATGCTTCAT+AGG - chr5_3:41854022-41854041 Msa0812030:CDS 40.0%
CGATGCTTCATAGGAATGTA+TGG - chr5_3:41854031-41854050 Msa0812030:intron 40.0%
!! CTTTGTTGGTTTCTTGAACC+AGG - chr5_3:41854092-41854111 Msa0812030:intron 40.0%
AGATCTTCATCTGGACTCAT+TGG - chr5_3:41854176-41854195 Msa0812030:intron 40.0%
! CATCTGGACTCATTGGTTAT+TGG - chr5_3:41854183-41854202 Msa0812030:intron 40.0%
! ACTCATTGGTTATTGGAAGC+AGG - chr5_3:41854190-41854209 Msa0812030:intron 40.0%
! CTCATTGGTTATTGGAAGCA+GGG - chr5_3:41854191-41854210 Msa0812030:intron 40.0%
! TCATTGGTTATTGGAAGCAG+GGG - chr5_3:41854192-41854211 Msa0812030:intron 40.0%
! GGGGTTTAGGTTCCATTAAA+AGG - chr5_3:41854211-41854230 Msa0812030:intron 40.0%
ATTCCCTTGACTACAGTAAC+CGG + chr5_3:41854397-41854416 None:intergenic 40.0%
GAATGCAACCTTCCAAGTTT+AGG - chr5_3:41854413-41854432 Msa0812030:intron 40.0%
! GCTAGCCAAAGCATTTCAAA+AGG + chr5_3:41854517-41854536 None:intergenic 40.0%
ATTGTGCCTCGGAATTTCAG+TGG + chr5_3:41853036-41853055 None:intergenic 45.0%
!! TTCCTAGGAGTATTGTGCCT+CGG + chr5_3:41853047-41853066 None:intergenic 45.0%
! CTTTCTAGTGTGATCAGCTG+AGG + chr5_3:41853200-41853219 None:intergenic 45.0%
CTCAGCTGATCACACTAGAA+AGG - chr5_3:41853198-41853217 Msa0812030:three_prime_UTR 45.0%
ACATCGAAACCCTGATGTTG+CGG + chr5_3:41853381-41853400 None:intergenic 45.0%
! TGGTGCCTCTAGAGGAATTA+AGG - chr5_3:41853718-41853737 Msa0812030:intron 45.0%
! GGTGCCTCTAGAGGAATTAA+GGG - chr5_3:41853719-41853738 Msa0812030:intron 45.0%
TGGTTTCTTGAACCAGGCAA+TGG - chr5_3:41854098-41854117 Msa0812030:intron 45.0%
!! TGGTAGTGGCAAAGGTGTAT+TGG - chr5_3:41854118-41854137 Msa0812030:intron 45.0%
! GGTAGTGGCAAAGGTGTATT+GGG - chr5_3:41854119-41854138 Msa0812030:intron 45.0%
! GTAGTGGCAAAGGTGTATTG+GGG - chr5_3:41854120-41854139 Msa0812030:intron 45.0%
GCATTGCACAATTTCTCCCT+TGG + chr5_3:41854150-41854169 None:intergenic 45.0%
TGCTGTGCAAGATCTTCATC+TGG - chr5_3:41854167-41854186 Msa0812030:intron 45.0%
GTTATTGGAAGCAGGGGTTT+AGG - chr5_3:41854198-41854217 Msa0812030:intron 45.0%
TTCCCTTGACTACAGTAACC+GGG + chr5_3:41854396-41854415 None:intergenic 45.0%
ATTGTGCCTCGGAATTTCAG+TGG + chr5_3:41853036-41853055 None:intergenic 45.0%
!! TTCCTAGGAGTATTGTGCCT+CGG + chr5_3:41853047-41853066 None:intergenic 45.0%
! CTTTCTAGTGTGATCAGCTG+AGG + chr5_3:41853200-41853219 None:intergenic 45.0%
CTCAGCTGATCACACTAGAA+AGG - chr5_3:41853198-41853217 Msa0812030:three_prime_UTR 45.0%
ACATCGAAACCCTGATGTTG+CGG + chr5_3:41853381-41853400 None:intergenic 45.0%
! TGGTGCCTCTAGAGGAATTA+AGG - chr5_3:41853718-41853737 Msa0812030:intron 45.0%
! GGTGCCTCTAGAGGAATTAA+GGG - chr5_3:41853719-41853738 Msa0812030:intron 45.0%
TGGTTTCTTGAACCAGGCAA+TGG - chr5_3:41854098-41854117 Msa0812030:intron 45.0%
!! TGGTAGTGGCAAAGGTGTAT+TGG - chr5_3:41854118-41854137 Msa0812030:intron 45.0%
! GGTAGTGGCAAAGGTGTATT+GGG - chr5_3:41854119-41854138 Msa0812030:intron 45.0%
! GTAGTGGCAAAGGTGTATTG+GGG - chr5_3:41854120-41854139 Msa0812030:intron 45.0%
GCATTGCACAATTTCTCCCT+TGG + chr5_3:41854150-41854169 None:intergenic 45.0%
TGCTGTGCAAGATCTTCATC+TGG - chr5_3:41854167-41854186 Msa0812030:intron 45.0%
GTTATTGGAAGCAGGGGTTT+AGG - chr5_3:41854198-41854217 Msa0812030:intron 45.0%
TTCCCTTGACTACAGTAACC+GGG + chr5_3:41854396-41854415 None:intergenic 45.0%
TTCCGAGGCACAATACTCCT+AGG - chr5_3:41853042-41853061 Msa0812030:three_prime_UTR 50.0%
TATACGACTGTGGTCTGGAC+CGG - chr5_3:41853354-41853373 Msa0812030:CDS 50.0%
CTTGAACCAGGCAATGGTAG+TGG - chr5_3:41854104-41854123 Msa0812030:intron 50.0%
AGGTGTATTGGGGAGATCCA+AGG - chr5_3:41854130-41854149 Msa0812030:intron 50.0%
GGTGTATTGGGGAGATCCAA+GGG - chr5_3:41854131-41854150 Msa0812030:intron 50.0%
AGGCAGTGTTAGCAAGCATG+TGG - chr5_3:41854354-41854373 Msa0812030:intron 50.0%
GGTGACAAATGCTTCTTGCC+CGG - chr5_3:41854375-41854394 Msa0812030:intron 50.0%
TTGCCCGGTTACTGTAGTCA+AGG - chr5_3:41854390-41854409 Msa0812030:intron 50.0%
TGCCCGGTTACTGTAGTCAA+GGG - chr5_3:41854391-41854410 Msa0812030:intron 50.0%
TTCCGAGGCACAATACTCCT+AGG - chr5_3:41853042-41853061 Msa0812030:three_prime_UTR 50.0%
TATACGACTGTGGTCTGGAC+CGG - chr5_3:41853354-41853373 Msa0812030:CDS 50.0%
CTTGAACCAGGCAATGGTAG+TGG - chr5_3:41854104-41854123 Msa0812030:intron 50.0%
AGGTGTATTGGGGAGATCCA+AGG - chr5_3:41854130-41854149 Msa0812030:intron 50.0%
GGTGTATTGGGGAGATCCAA+GGG - chr5_3:41854131-41854150 Msa0812030:intron 50.0%
AGGCAGTGTTAGCAAGCATG+TGG - chr5_3:41854354-41854373 Msa0812030:intron 50.0%
GGTGACAAATGCTTCTTGCC+CGG - chr5_3:41854375-41854394 Msa0812030:intron 50.0%
TTGCCCGGTTACTGTAGTCA+AGG - chr5_3:41854390-41854409 Msa0812030:intron 50.0%
TGCCCGGTTACTGTAGTCAA+GGG - chr5_3:41854391-41854410 Msa0812030:intron 50.0%
CTAGTGTGATCAGCTGAGGG+AGG + chr5_3:41853196-41853215 None:intergenic 55.0%
ATACGACTGTGGTCTGGACC+GGG - chr5_3:41853355-41853374 Msa0812030:CDS 55.0%
CGCAGCCCAGATTGCATTCA+TGG - chr5_3:41853571-41853590 Msa0812030:CDS 55.0%
!! TGCAGCTTTGGTGCCTCTAG+AGG - chr5_3:41853710-41853729 Msa0812030:intron 55.0%
CCTTTGCCACTACCATTGCC+TGG + chr5_3:41854113-41854132 None:intergenic 55.0%
!! CCAGGCAATGGTAGTGGCAA+AGG - chr5_3:41854110-41854129 Msa0812030:intron 55.0%
CTAGTGTGATCAGCTGAGGG+AGG + chr5_3:41853196-41853215 None:intergenic 55.0%
ATACGACTGTGGTCTGGACC+GGG - chr5_3:41853355-41853374 Msa0812030:CDS 55.0%
CGCAGCCCAGATTGCATTCA+TGG - chr5_3:41853571-41853590 Msa0812030:CDS 55.0%
!! TGCAGCTTTGGTGCCTCTAG+AGG - chr5_3:41853710-41853729 Msa0812030:intron 55.0%
CCTTTGCCACTACCATTGCC+TGG + chr5_3:41854113-41854132 None:intergenic 55.0%
!! CCAGGCAATGGTAGTGGCAA+AGG - chr5_3:41854110-41854129 Msa0812030:intron 55.0%
GAAACCCTGATGTTGCGGCC+CGG + chr5_3:41853376-41853395 None:intergenic 60.0%
GAAACCCTGATGTTGCGGCC+CGG + chr5_3:41853376-41853395 None:intergenic 60.0%
TGGACCGGGCCGCAACATCA+GGG - chr5_3:41853369-41853388 Msa0812030:CDS 65.0%
TGGACCGGGCCGCAACATCA+GGG - chr5_3:41853369-41853388 Msa0812030:CDS 65.0%
CTGGACCGGGCCGCAACATC+AGG - chr5_3:41853368-41853387 Msa0812030:CDS 70.0%
CTGGACCGGGCCGCAACATC+AGG - chr5_3:41853368-41853387 Msa0812030:CDS 70.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 41853028 41854734 41853028 ID=Msa0812030;Name=Msa0812030
chr5_3 mRNA 41853028 41854734 41853028 ID=Msa0812030-mRNA-1;Parent=Msa0812030;Name=Msa0812030-mRNA-1;_AED=0.09;_eAED=0.09;_QI=42|1|1|1|1|1|4|293|166
chr5_3 exon 41853028 41853417 41853028 ID=Msa0812030-mRNA-1:exon:12844;Parent=Msa0812030-mRNA-1
chr5_3 exon 41853530 41853648 41853530 ID=Msa0812030-mRNA-1:exon:12843;Parent=Msa0812030-mRNA-1
chr5_3 exon 41853938 41854047 41853938 ID=Msa0812030-mRNA-1:exon:12842;Parent=Msa0812030-mRNA-1
chr5_3 exon 41854518 41854734 41854518 ID=Msa0812030-mRNA-1:exon:12841;Parent=Msa0812030-mRNA-1
chr5_3 five_prime_UTR 41854693 41854734 41854693 ID=Msa0812030-mRNA-1:five_prime_utr;Parent=Msa0812030-mRNA-1
chr5_3 CDS 41854518 41854692 41854518 ID=Msa0812030-mRNA-1:cds;Parent=Msa0812030-mRNA-1
chr5_3 CDS 41853938 41854047 41853938 ID=Msa0812030-mRNA-1:cds;Parent=Msa0812030-mRNA-1
chr5_3 CDS 41853530 41853648 41853530 ID=Msa0812030-mRNA-1:cds;Parent=Msa0812030-mRNA-1
chr5_3 CDS 41853321 41853417 41853321 ID=Msa0812030-mRNA-1:cds;Parent=Msa0812030-mRNA-1
chr5_3 three_prime_UTR 41853028 41853320 41853028 ID=Msa0812030-mRNA-1:three_prime_utr;Parent=Msa0812030-mRNA-1
Gene Sequence

>Msa0812030

ATGGCAAAAGCACACATAGTTGGAGTAGCTATGGACTTCTCTCCTACCAGTAAATTAGCACTTAGATGGGCTGTTGATAATCTGATCAACAAAAATGATCAAATCATCATGATCAATGTTCAGCCTCCCTCAGCTGATCACACTAGAAAGGAGCTTTTTGAAGACACTGGATCACCTTTGGTGCCTCTAGAGGAATTAAGGGAGATAAACTTTACAAAGCAATATGGAATTGCTAAGGATCCTGAAGTCATAGATATTCTTGAAACTGCCTCAAAAACCAAAGGGGCAATGGTAGTGGCAAAGGTGTATTGGGGAGATCCAAGGGAGAAATTGTGCAATGCTGTGCAAGATCTTCATCTGGACTCATTGGTTATTGGAAGCAGGGGTTTAGGTTCCATTAAAAGTGTGTTGTTAGGCAGTGTTAGCAAGCATGTGGTGACAAATGCTTCTTGCCCGGTTACTGTAGTCAAGGGAATGCAACCTTCCAAGTTTAGGCATTGA
Protein sequence

>Msa0812030

MAKAHIVGVAMDFSPTSKLALRWAVDNLINKNDQIIMINVQPPSADHTRKELFEDTGSPLVPLEELREINFTKQYGIAKDPEVIDILETASKTKGAMVVAKVYWGDPREKLCNAVQDLHLDSLVIGSRGLGSIKSVLLGSVSKHVVTNASCPVTVVKGMQPSKFRH*