AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0856060

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0856060 MtrunA17_Chr5g0433951 95.783 166 7 0 1 166 1 166 8.64e-117 327
Msa0856060 MtrunA17_Chr4g0008801 54.545 165 75 0 1 165 1 165 3.51e-60 184
Msa0856060 MtrunA17_Chr4g0032261 54.777 157 71 0 1 157 1 157 5.12e-59 181
Msa0856060 MtrunA17_Chr7g0240701 49.045 157 80 0 1 157 1 157 3.40e-54 169
Msa0856060 MtrunA17_Chr2g0294421 51.592 157 76 0 1 157 1 157 1.49e-52 164
Msa0856060 MtrunA17_Chr1g0194801 35.152 165 91 6 3 161 4 158 6.98e-21 83.6
Msa0856060 MtrunA17_Chr1g0192651 35.220 159 87 6 4 157 8 155 4.85e-19 79.3
Msa0856060 MtrunA17_Chr5g0427291 36.747 166 89 5 6 158 9 171 3.04e-18 77.4
Msa0856060 MtrunA17_Chr4g0001641 54.098 61 28 0 98 158 6 66 1.14e-17 73.2
Msa0856060 MtrunA17_Chr1g0176311 26.596 188 89 5 7 166 24 190 2.27e-16 72.8
Msa0856060 MtrunA17_Chr1g0176321 26.984 189 93 4 7 166 5 177 1.73e-15 70.1
Msa0856060 MtrunA17_Chr6g0450171 30.612 147 91 3 14 157 24 162 3.40e-15 69.3
Msa0856060 MtrunA17_Chr2g0323291 30.061 163 98 4 5 157 25 181 1.08e-13 66.2
Msa0856060 MtrunA17_Chr1g0195661 29.032 155 95 5 9 157 16 161 3.78e-13 63.9
Msa0856060 MtrunA17_Chr6g0464761 50.000 56 21 1 98 153 15 63 3.81e-12 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0856060 AT3G03270.2 70.253 158 47 0 1 158 1 158 2.76e-79 232
Msa0856060 AT3G03270.1 68.657 134 42 0 1 134 1 134 2.02e-63 194
Msa0856060 AT3G53990.1 50.318 157 78 0 1 157 1 157 2.98e-54 169
Msa0856060 AT3G17020.1 45.513 156 84 1 4 158 6 161 9.56e-49 155
Msa0856060 AT3G53990.2 42.857 98 56 0 1 98 1 98 2.48e-24 92.0
Msa0856060 AT3G62550.1 34.969 163 87 5 3 157 5 156 8.99e-19 78.6
Msa0856060 AT1G09740.2 37.107 159 88 4 7 157 10 164 1.16e-18 78.6
Msa0856060 AT1G09740.1 37.107 159 88 4 7 157 10 164 1.16e-18 78.6
Msa0856060 AT3G58450.2 31.707 164 85 6 7 157 32 181 7.63e-17 74.7
Msa0856060 AT3G17020.2 35.870 92 58 1 4 94 6 97 9.04e-17 72.4
Msa0856060 AT1G11360.2 29.487 156 107 1 5 157 38 193 2.98e-16 73.9
Msa0856060 AT1G11360.1 29.487 156 107 1 5 157 38 193 2.98e-16 73.9
Msa0856060 AT1G11360.4 29.487 156 107 1 5 157 38 193 2.98e-16 73.9
Msa0856060 AT1G11360.3 29.487 156 107 1 5 157 38 193 2.98e-16 73.9
Msa0856060 AT1G68300.1 32.468 154 93 4 7 157 12 157 3.19e-16 72.0
Msa0856060 AT3G01520.1 29.932 147 92 3 14 157 24 162 3.72e-16 72.4
Msa0856060 AT3G58450.1 31.953 169 85 6 7 157 32 188 6.60e-16 72.0
Msa0856060 AT5G14680.1 29.252 147 93 3 14 157 24 162 1.73e-15 70.5
Msa0856060 AT3G11930.4 29.560 159 101 2 9 157 37 194 6.56e-15 69.3
Msa0856060 AT3G11930.1 29.560 159 99 3 9 157 37 192 1.43e-14 68.6
Msa0856060 AT3G11930.2 29.560 159 100 3 9 157 37 193 3.01e-14 67.8
Msa0856060 AT2G47710.1 28.571 154 96 4 9 157 12 156 4.51e-11 58.5
Msa0856060 AT5G54430.3 29.530 149 90 3 5 144 48 190 8.70e-11 58.9
Msa0856060 AT5G54430.6 29.530 149 90 3 5 144 48 190 8.70e-11 58.9
Msa0856060 AT5G54430.1 29.530 149 90 3 5 144 48 190 8.79e-11 58.9
Msa0856060 AT5G54430.4 29.530 149 90 3 5 144 48 190 8.79e-11 58.9
Msa0856060 AT5G54430.2 29.530 149 90 3 5 144 48 190 9.40e-11 58.9
Msa0856060 AT5G54430.5 29.530 149 90 3 5 144 48 190 9.40e-11 58.9

Find 54 sgRNAs with CRISPR-Local

Find 194 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGTGCCTCTAGAGGAATTA+AGG 0.233780 5_4:-50418892 Msa0856060:CDS
CATCTGGACTCATTGGTTAT+TGG 0.247760 5_4:-50418428 Msa0856060:CDS
GGTAGTGGCAAAGGTGTATT+GGG 0.310523 5_4:-50418492 Msa0856060:CDS
ATGATTATTAATATTTACTT+TGG 0.311087 5_4:-50418163 Msa0856060:three_prime_UTR
CTTCATTTCAGTGTGTTGTT+AGG 0.317283 5_4:-50418277 Msa0856060:intron
GTTATTGGAAGCAGGGGTTT+AGG 0.321337 5_4:-50418413 Msa0856060:CDS
GGGGTTTAGGTTCCATTAAA+AGG 0.346706 5_4:-50418400 Msa0856060:intron
TCAAGAATATCTATGACTTC+AGG 0.358570 5_4:+50418830 None:intergenic
CCAGCAAATTAGCACTTAGA+TGG 0.389656 5_4:-50419494 Msa0856060:CDS
CCATCTAAGTGCTAATTTGC+TGG 0.396841 5_4:+50419494 None:intergenic
AGATCTTCATCTGGACTCAT+TGG 0.398485 5_4:-50418435 Msa0856060:CDS
CATGAAACAATTGTCACATA+AGG 0.399976 5_4:-50417912 Msa0856060:three_prime_UTR
AAACAACATAAATACCCCTT+TGG 0.402357 5_4:+50418793 None:intergenic
ATAAACTTTACAAAGCAATA+TGG 0.410129 5_4:-50418866 Msa0856060:CDS
ATTCCCTTGACTACAGTAAC+CGG 0.414500 5_4:+50418217 None:intergenic
TGATTATTAATATTTACTTT+GGG 0.421374 5_4:-50418162 Msa0856060:three_prime_UTR
TGGTAGTGGCAAAGGTGTAT+TGG 0.429624 5_4:-50418493 Msa0856060:CDS
TGTGATCAGCTGAGGGAGGC+TGG 0.432159 5_4:+50419421 None:intergenic
GCATTGCACAATTTCTCCCT+TGG 0.434268 5_4:+50418464 None:intergenic
GGTGCCTCTAGAGGAATTAA+GGG 0.447240 5_4:-50418891 Msa0856060:CDS
TGCTGTGCAAGATCTTCATC+TGG 0.451178 5_4:-50418444 Msa0856060:CDS
ACTCAGATAAATTGTAATGA+TGG 0.464485 5_4:+50418013 None:intergenic
ATGAAACAATTGTCACATAA+GGG 0.468956 5_4:-50417911 Msa0856060:three_prime_UTR
CTCATTGGTTATTGGAAGCA+GGG 0.470129 5_4:-50418420 Msa0856060:CDS
ACTCATTGGTTATTGGAAGC+AGG 0.470238 5_4:-50418421 Msa0856060:CDS
ATGGCAAAAGCACGCATAGT+TGG 0.471789 5_4:-50419540 Msa0856060:CDS
CTTGAACCAGGCAATGGTAG+TGG 0.472382 5_4:-50418507 Msa0856060:intron
ATCAAATCAATGCCTAGACT+TGG 0.522276 5_4:+50418186 None:intergenic
CTTTCTAGTGTGATCAGCTG+AGG 0.523922 5_4:+50419413 None:intergenic
CTCAGCTGATCACACTAGAA+AGG 0.525726 5_4:-50419412 Msa0856060:CDS
ACACAACTAATAAGAAAAGA+TGG 0.529169 5_4:-50419559 Msa0856060:exon
GCTAGCCAAAGCATTTCAAA+AGG 0.531277 5_4:+50418097 None:intergenic
TGCAGCTTTGGTGCCTCTAG+AGG 0.535834 5_4:-50418900 Msa0856060:intron
CCAGGCAATGGTAGTGGCAA+AGG 0.540554 5_4:-50418501 Msa0856060:intron
ATCTCCCTTAATTCCTCTAG+AGG 0.547571 5_4:+50418887 None:intergenic
TTGCCCGGTTACTGTAGTCA+AGG 0.560158 5_4:-50418221 Msa0856060:CDS
AGGTGTATTGGGGAGATCCA+AGG 0.560692 5_4:-50418481 Msa0856060:CDS
GAATGCAACCTTCCAAGTCT+AGG 0.571630 5_4:-50418198 Msa0856060:CDS
AAAGCAATATGGAATTGCTA+AGG 0.573242 5_4:-50418855 Msa0856060:CDS
ACGCATAGTTGGAGTAGCTA+TGG 0.578284 5_4:-50419529 Msa0856060:CDS
TGCCCGGTTACTGTAGTCAA+GGG 0.580340 5_4:-50418220 Msa0856060:CDS
TTTCTAGTGTGATCAGCTGA+GGG 0.583250 5_4:+50419414 None:intergenic
GGTGACAAATGCTTCTTGCC+CGG 0.585729 5_4:-50418236 Msa0856060:CDS
AATCAATGCCTAGACTTGGA+AGG 0.590106 5_4:+50418190 None:intergenic
GGTGTATTGGGGAGATCCAA+GGG 0.593103 5_4:-50418480 Msa0856060:CDS
CTAAGTGCTAATTTGCTGGT+AGG 0.598950 5_4:+50419498 None:intergenic
CTAGTGTGATCAGCTGAGGG+AGG 0.611787 5_4:+50419417 None:intergenic
TCATTGGTTATTGGAAGCAG+GGG 0.616498 5_4:-50418419 Msa0856060:CDS
AAGAAACCACTGAAATTCAG+AGG 0.617542 5_4:-50419583 Msa0856060:five_prime_UTR
TGTGTGCCTCTGAATTTCAG+TGG 0.620282 5_4:+50419577 None:intergenic
TTCCCTTGACTACAGTAACC+GGG 0.638321 5_4:+50418218 None:intergenic
GTAGTGGCAAAGGTGTATTG+GGG 0.678633 5_4:-50418491 Msa0856060:CDS
ATGTGTCATATATTGATGAG+AGG 0.679189 5_4:-50417963 Msa0856060:three_prime_UTR
AGGCAGTGTTAGCAAGCATG+TGG 0.730937 5_4:-50418257 Msa0856060:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGATTATTAATATTTACTT+TGG - chr5_4:50419318-50419337 Msa0856060:intron 10.0%
!! TGATTATTAATATTTACTTT+GGG - chr5_4:50419319-50419338 Msa0856060:intron 10.0%
!! ATGATTATTAATATTTACTT+TGG - chr5_4:50419318-50419337 Msa0856060:intron 10.0%
!! TGATTATTAATATTTACTTT+GGG - chr5_4:50419319-50419338 Msa0856060:intron 10.0%
!!! TTTTTTTCTCATGTTATTAT+CGG + chr5_4:50418813-50418832 None:intergenic 15.0%
!!! TTTTTTTCTCATGTTATTAT+CGG + chr5_4:50418813-50418832 None:intergenic 15.0%
!! ATAAACTTTACAAAGCAATA+TGG - chr5_4:50418615-50418634 Msa0856060:intron 20.0%
!!! TAAACTATAGTACCTTTTAA+TGG + chr5_4:50419096-50419115 None:intergenic 20.0%
!! ATAAACTTTACAAAGCAATA+TGG - chr5_4:50418615-50418634 Msa0856060:intron 20.0%
!!! TAAACTATAGTACCTTTTAA+TGG + chr5_4:50419096-50419115 None:intergenic 20.0%
! ACACAACTAATAAGAAAAGA+TGG - chr5_4:50417922-50417941 Msa0856060:three_prime_UTR 25.0%
! TAAAAGAAAGAGAATGAAAG+AGG + chr5_4:50418126-50418145 None:intergenic 25.0%
!! ACTGATGTTATTTAAAAGCT+TGG + chr5_4:50418160-50418179 None:intergenic 25.0%
!! AAGATTTTAAATAGCAGAAG+TGG + chr5_4:50418363-50418382 None:intergenic 25.0%
! TATGTTGTATAGGTTTATCT+TGG - chr5_4:50418499-50418518 Msa0856060:CDS 25.0%
!!! TGACAATTTTGTGTTTTGTT+TGG - chr5_4:50418549-50418568 Msa0856060:intron 25.0%
!!! GATTTTTTATGTGTCTTTGT+TGG - chr5_4:50418948-50418967 Msa0856060:intron 25.0%
!! AGCTTGAATAATTGTCTTTT+TGG - chr5_4:50419404-50419423 Msa0856060:CDS 25.0%
! ACTCAGATAAATTGTAATGA+TGG + chr5_4:50419471-50419490 None:intergenic 25.0%
! ATGAAACAATTGTCACATAA+GGG - chr5_4:50419570-50419589 Msa0856060:five_prime_UTR 25.0%
! ACACAACTAATAAGAAAAGA+TGG - chr5_4:50417922-50417941 Msa0856060:three_prime_UTR 25.0%
! TAAAAGAAAGAGAATGAAAG+AGG + chr5_4:50418126-50418145 None:intergenic 25.0%
!! ACTGATGTTATTTAAAAGCT+TGG + chr5_4:50418160-50418179 None:intergenic 25.0%
!! AAGATTTTAAATAGCAGAAG+TGG + chr5_4:50418363-50418382 None:intergenic 25.0%
! TATGTTGTATAGGTTTATCT+TGG - chr5_4:50418499-50418518 Msa0856060:CDS 25.0%
!!! TGACAATTTTGTGTTTTGTT+TGG - chr5_4:50418549-50418568 Msa0856060:intron 25.0%
!!! GATTTTTTATGTGTCTTTGT+TGG - chr5_4:50418948-50418967 Msa0856060:intron 25.0%
!! AGCTTGAATAATTGTCTTTT+TGG - chr5_4:50419404-50419423 Msa0856060:CDS 25.0%
! ACTCAGATAAATTGTAATGA+TGG + chr5_4:50419471-50419490 None:intergenic 25.0%
! ATGAAACAATTGTCACATAA+GGG - chr5_4:50419570-50419589 Msa0856060:five_prime_UTR 25.0%
CTGTTAAATTTATACGACTG+TGG - chr5_4:50418215-50418234 Msa0856060:CDS 30.0%
TTCATAACTACAGAAAACCA+CGG + chr5_4:50418314-50418333 None:intergenic 30.0%
TGCTGAAACAATCTCATTTA+TGG - chr5_4:50418383-50418402 Msa0856060:intron 30.0%
! GATTTTTGATGTCTCAACAA+CGG + chr5_4:50418415-50418434 None:intergenic 30.0%
AAAATCCATGAATGCAATCT+GGG + chr5_4:50418450-50418469 None:intergenic 30.0%
AAAAATCCATGAATGCAATC+TGG + chr5_4:50418451-50418470 None:intergenic 30.0%
GCACATACTATATGTTGTAT+AGG - chr5_4:50418489-50418508 Msa0856060:CDS 30.0%
AAAGCAATATGGAATTGCTA+AGG - chr5_4:50418626-50418645 Msa0856060:intron 30.0%
TCAAGAATATCTATGACTTC+AGG + chr5_4:50418654-50418673 None:intergenic 30.0%
AAACAACATAAATACCCCTT+TGG + chr5_4:50418691-50418710 None:intergenic 30.0%
TGGTGTAGTTAACACATATA+TGG - chr5_4:50418923-50418942 Msa0856060:intron 30.0%
!! GAATAATTGTCTTTTTGGCA+TGG - chr5_4:50419409-50419428 Msa0856060:CDS 30.0%
ATGTGTCATATATTGATGAG+AGG - chr5_4:50419518-50419537 Msa0856060:CDS 30.0%
CATGAAACAATTGTCACATA+AGG - chr5_4:50419569-50419588 Msa0856060:five_prime_UTR 30.0%
CTGTTAAATTTATACGACTG+TGG - chr5_4:50418215-50418234 Msa0856060:CDS 30.0%
TTCATAACTACAGAAAACCA+CGG + chr5_4:50418314-50418333 None:intergenic 30.0%
TGCTGAAACAATCTCATTTA+TGG - chr5_4:50418383-50418402 Msa0856060:intron 30.0%
! GATTTTTGATGTCTCAACAA+CGG + chr5_4:50418415-50418434 None:intergenic 30.0%
AAAATCCATGAATGCAATCT+GGG + chr5_4:50418450-50418469 None:intergenic 30.0%
AAAAATCCATGAATGCAATC+TGG + chr5_4:50418451-50418470 None:intergenic 30.0%
GCACATACTATATGTTGTAT+AGG - chr5_4:50418489-50418508 Msa0856060:CDS 30.0%
AAAGCAATATGGAATTGCTA+AGG - chr5_4:50418626-50418645 Msa0856060:intron 30.0%
TCAAGAATATCTATGACTTC+AGG + chr5_4:50418654-50418673 None:intergenic 30.0%
AAACAACATAAATACCCCTT+TGG + chr5_4:50418691-50418710 None:intergenic 30.0%
TGGTGTAGTTAACACATATA+TGG - chr5_4:50418923-50418942 Msa0856060:intron 30.0%
!! GAATAATTGTCTTTTTGGCA+TGG - chr5_4:50419409-50419428 Msa0856060:CDS 30.0%
ATGTGTCATATATTGATGAG+AGG - chr5_4:50419518-50419537 Msa0856060:CDS 30.0%
CATGAAACAATTGTCACATA+AGG - chr5_4:50419569-50419588 Msa0856060:five_prime_UTR 30.0%
AAATTTATACGACTGTGGTC+TGG - chr5_4:50418220-50418239 Msa0856060:CDS 35.0%
!! AGTCGATTTTGCCTCAATTA+TGG + chr5_4:50418283-50418302 None:intergenic 35.0%
CATATAGTATGTGCTAAGCA+AGG + chr5_4:50418483-50418502 None:intergenic 35.0%
AAACTGCCTCAAAAACCAAA+GGG - chr5_4:50418673-50418692 Msa0856060:intron 35.0%
!!! AAATACCCCTTTGGTTTTTG+AGG + chr5_4:50418682-50418701 None:intergenic 35.0%
! CTTCATTTCAGTGTGTTGTT+AGG - chr5_4:50419204-50419223 Msa0856060:intron 35.0%
ATCAAATCAATGCCTAGACT+TGG + chr5_4:50419298-50419317 None:intergenic 35.0%
!!! CTGTACCTTTTGAAATGCTT+TGG - chr5_4:50419379-50419398 Msa0856060:intron 35.0%
AAATTTATACGACTGTGGTC+TGG - chr5_4:50418220-50418239 Msa0856060:CDS 35.0%
!! AGTCGATTTTGCCTCAATTA+TGG + chr5_4:50418283-50418302 None:intergenic 35.0%
CATATAGTATGTGCTAAGCA+AGG + chr5_4:50418483-50418502 None:intergenic 35.0%
AAACTGCCTCAAAAACCAAA+GGG - chr5_4:50418673-50418692 Msa0856060:intron 35.0%
!!! AAATACCCCTTTGGTTTTTG+AGG + chr5_4:50418682-50418701 None:intergenic 35.0%
! CTTCATTTCAGTGTGTTGTT+AGG - chr5_4:50419204-50419223 Msa0856060:intron 35.0%
ATCAAATCAATGCCTAGACT+TGG + chr5_4:50419298-50419317 None:intergenic 35.0%
!!! CTGTACCTTTTGAAATGCTT+TGG - chr5_4:50419379-50419398 Msa0856060:intron 35.0%
!! CTAAGTGCTAATTTGCTGGT+AGG + chr5_4:50417986-50418005 None:intergenic 40.0%
! CCATCTAAGTGCTAATTTGC+TGG + chr5_4:50417990-50418009 None:intergenic 40.0%
CCAGCAAATTAGCACTTAGA+TGG - chr5_4:50417987-50418006 Msa0856060:three_prime_UTR 40.0%
! TTTCTAGTGTGATCAGCTGA+GGG + chr5_4:50418070-50418089 None:intergenic 40.0%
!! AAGGAGCTTTTTGAAGACAC+TGG - chr5_4:50418088-50418107 Msa0856060:three_prime_UTR 40.0%
TGTCTCTACGACCATAATTG+AGG - chr5_4:50418269-50418288 Msa0856060:CDS 40.0%
AAATCGACTATAAGTGACCG+TGG - chr5_4:50418294-50418313 Msa0856060:intron 40.0%
!! TGGTTGATGATTTGCAGCTT+TGG - chr5_4:50418569-50418588 Msa0856060:intron 40.0%
ATCTCCCTTAATTCCTCTAG+AGG + chr5_4:50418597-50418616 None:intergenic 40.0%
GAAACTGCCTCAAAAACCAA+AGG - chr5_4:50418672-50418691 Msa0856060:intron 40.0%
AACTGCCTCAAAAACCAAAG+GGG - chr5_4:50418674-50418693 Msa0856060:intron 40.0%
TCTGATGTCTAAATCTGCGT+CGG + chr5_4:50418777-50418796 None:intergenic 40.0%
CTTGTATCTGTGTCAGATAG+TGG - chr5_4:50418858-50418877 Msa0856060:CDS 40.0%
!! TTGAAGTGTCGATGCTTCAT+AGG - chr5_4:50418894-50418913 Msa0856060:CDS 40.0%
CGATGCTTCATAGGAATGTA+TGG - chr5_4:50418903-50418922 Msa0856060:intron 40.0%
!! CTTTGTTGGTTTCTTGAACC+AGG - chr5_4:50418962-50418981 Msa0856060:intron 40.0%
AGATCTTCATCTGGACTCAT+TGG - chr5_4:50419046-50419065 Msa0856060:intron 40.0%
! CATCTGGACTCATTGGTTAT+TGG - chr5_4:50419053-50419072 Msa0856060:intron 40.0%
! ACTCATTGGTTATTGGAAGC+AGG - chr5_4:50419060-50419079 Msa0856060:intron 40.0%
! CTCATTGGTTATTGGAAGCA+GGG - chr5_4:50419061-50419080 Msa0856060:intron 40.0%
! TCATTGGTTATTGGAAGCAG+GGG - chr5_4:50419062-50419081 Msa0856060:intron 40.0%
! GGGGTTTAGGTTCCATTAAA+AGG - chr5_4:50419081-50419100 Msa0856060:intron 40.0%
ATTCCCTTGACTACAGTAAC+CGG + chr5_4:50419267-50419286 None:intergenic 40.0%
AATCAATGCCTAGACTTGGA+AGG + chr5_4:50419294-50419313 None:intergenic 40.0%
! GCTAGCCAAAGCATTTCAAA+AGG + chr5_4:50419387-50419406 None:intergenic 40.0%
!! CTAAGTGCTAATTTGCTGGT+AGG + chr5_4:50417986-50418005 None:intergenic 40.0%
! CCATCTAAGTGCTAATTTGC+TGG + chr5_4:50417990-50418009 None:intergenic 40.0%
CCAGCAAATTAGCACTTAGA+TGG - chr5_4:50417987-50418006 Msa0856060:three_prime_UTR 40.0%
! TTTCTAGTGTGATCAGCTGA+GGG + chr5_4:50418070-50418089 None:intergenic 40.0%
!! AAGGAGCTTTTTGAAGACAC+TGG - chr5_4:50418088-50418107 Msa0856060:three_prime_UTR 40.0%
TGTCTCTACGACCATAATTG+AGG - chr5_4:50418269-50418288 Msa0856060:CDS 40.0%
AAATCGACTATAAGTGACCG+TGG - chr5_4:50418294-50418313 Msa0856060:intron 40.0%
!! TGGTTGATGATTTGCAGCTT+TGG - chr5_4:50418569-50418588 Msa0856060:intron 40.0%
ATCTCCCTTAATTCCTCTAG+AGG + chr5_4:50418597-50418616 None:intergenic 40.0%
GAAACTGCCTCAAAAACCAA+AGG - chr5_4:50418672-50418691 Msa0856060:intron 40.0%
AACTGCCTCAAAAACCAAAG+GGG - chr5_4:50418674-50418693 Msa0856060:intron 40.0%
TCTGATGTCTAAATCTGCGT+CGG + chr5_4:50418777-50418796 None:intergenic 40.0%
CTTGTATCTGTGTCAGATAG+TGG - chr5_4:50418858-50418877 Msa0856060:CDS 40.0%
!! TTGAAGTGTCGATGCTTCAT+AGG - chr5_4:50418894-50418913 Msa0856060:CDS 40.0%
CGATGCTTCATAGGAATGTA+TGG - chr5_4:50418903-50418922 Msa0856060:intron 40.0%
!! CTTTGTTGGTTTCTTGAACC+AGG - chr5_4:50418962-50418981 Msa0856060:intron 40.0%
AGATCTTCATCTGGACTCAT+TGG - chr5_4:50419046-50419065 Msa0856060:intron 40.0%
! CATCTGGACTCATTGGTTAT+TGG - chr5_4:50419053-50419072 Msa0856060:intron 40.0%
! ACTCATTGGTTATTGGAAGC+AGG - chr5_4:50419060-50419079 Msa0856060:intron 40.0%
! CTCATTGGTTATTGGAAGCA+GGG - chr5_4:50419061-50419080 Msa0856060:intron 40.0%
! TCATTGGTTATTGGAAGCAG+GGG - chr5_4:50419062-50419081 Msa0856060:intron 40.0%
! GGGGTTTAGGTTCCATTAAA+AGG - chr5_4:50419081-50419100 Msa0856060:intron 40.0%
ATTCCCTTGACTACAGTAAC+CGG + chr5_4:50419267-50419286 None:intergenic 40.0%
AATCAATGCCTAGACTTGGA+AGG + chr5_4:50419294-50419313 None:intergenic 40.0%
! GCTAGCCAAAGCATTTCAAA+AGG + chr5_4:50419387-50419406 None:intergenic 40.0%
TGTGTGCCTCTGAATTTCAG+TGG + chr5_4:50417907-50417926 None:intergenic 45.0%
! ATGGCAAAAGCACGCATAGT+TGG - chr5_4:50417941-50417960 Msa0856060:three_prime_UTR 45.0%
ACGCATAGTTGGAGTAGCTA+TGG - chr5_4:50417952-50417971 Msa0856060:three_prime_UTR 45.0%
! CTTTCTAGTGTGATCAGCTG+AGG + chr5_4:50418071-50418090 None:intergenic 45.0%
CTCAGCTGATCACACTAGAA+AGG - chr5_4:50418069-50418088 Msa0856060:three_prime_UTR 45.0%
ACATCGAAACCCTGATGTTG+CGG + chr5_4:50418252-50418271 None:intergenic 45.0%
! TGGTGCCTCTAGAGGAATTA+AGG - chr5_4:50418589-50418608 Msa0856060:intron 45.0%
! GGTGCCTCTAGAGGAATTAA+GGG - chr5_4:50418590-50418609 Msa0856060:intron 45.0%
TGGTTTCTTGAACCAGGCAA+TGG - chr5_4:50418968-50418987 Msa0856060:intron 45.0%
!! TGGTAGTGGCAAAGGTGTAT+TGG - chr5_4:50418988-50419007 Msa0856060:intron 45.0%
! GGTAGTGGCAAAGGTGTATT+GGG - chr5_4:50418989-50419008 Msa0856060:intron 45.0%
! GTAGTGGCAAAGGTGTATTG+GGG - chr5_4:50418990-50419009 Msa0856060:intron 45.0%
GCATTGCACAATTTCTCCCT+TGG + chr5_4:50419020-50419039 None:intergenic 45.0%
TGCTGTGCAAGATCTTCATC+TGG - chr5_4:50419037-50419056 Msa0856060:intron 45.0%
GTTATTGGAAGCAGGGGTTT+AGG - chr5_4:50419068-50419087 Msa0856060:intron 45.0%
TTCCCTTGACTACAGTAACC+GGG + chr5_4:50419266-50419285 None:intergenic 45.0%
GAATGCAACCTTCCAAGTCT+AGG - chr5_4:50419283-50419302 Msa0856060:intron 45.0%
TGTGTGCCTCTGAATTTCAG+TGG + chr5_4:50417907-50417926 None:intergenic 45.0%
! ATGGCAAAAGCACGCATAGT+TGG - chr5_4:50417941-50417960 Msa0856060:three_prime_UTR 45.0%
ACGCATAGTTGGAGTAGCTA+TGG - chr5_4:50417952-50417971 Msa0856060:three_prime_UTR 45.0%
! CTTTCTAGTGTGATCAGCTG+AGG + chr5_4:50418071-50418090 None:intergenic 45.0%
CTCAGCTGATCACACTAGAA+AGG - chr5_4:50418069-50418088 Msa0856060:three_prime_UTR 45.0%
ACATCGAAACCCTGATGTTG+CGG + chr5_4:50418252-50418271 None:intergenic 45.0%
! TGGTGCCTCTAGAGGAATTA+AGG - chr5_4:50418589-50418608 Msa0856060:intron 45.0%
! GGTGCCTCTAGAGGAATTAA+GGG - chr5_4:50418590-50418609 Msa0856060:intron 45.0%
TGGTTTCTTGAACCAGGCAA+TGG - chr5_4:50418968-50418987 Msa0856060:intron 45.0%
!! TGGTAGTGGCAAAGGTGTAT+TGG - chr5_4:50418988-50419007 Msa0856060:intron 45.0%
! GGTAGTGGCAAAGGTGTATT+GGG - chr5_4:50418989-50419008 Msa0856060:intron 45.0%
! GTAGTGGCAAAGGTGTATTG+GGG - chr5_4:50418990-50419009 Msa0856060:intron 45.0%
GCATTGCACAATTTCTCCCT+TGG + chr5_4:50419020-50419039 None:intergenic 45.0%
TGCTGTGCAAGATCTTCATC+TGG - chr5_4:50419037-50419056 Msa0856060:intron 45.0%
GTTATTGGAAGCAGGGGTTT+AGG - chr5_4:50419068-50419087 Msa0856060:intron 45.0%
TTCCCTTGACTACAGTAACC+GGG + chr5_4:50419266-50419285 None:intergenic 45.0%
GAATGCAACCTTCCAAGTCT+AGG - chr5_4:50419283-50419302 Msa0856060:intron 45.0%
TATACGACTGTGGTCTGGAC+CGG - chr5_4:50418225-50418244 Msa0856060:CDS 50.0%
CTTGAACCAGGCAATGGTAG+TGG - chr5_4:50418974-50418993 Msa0856060:intron 50.0%
AGGTGTATTGGGGAGATCCA+AGG - chr5_4:50419000-50419019 Msa0856060:intron 50.0%
GGTGTATTGGGGAGATCCAA+GGG - chr5_4:50419001-50419020 Msa0856060:intron 50.0%
AGGCAGTGTTAGCAAGCATG+TGG - chr5_4:50419224-50419243 Msa0856060:intron 50.0%
GGTGACAAATGCTTCTTGCC+CGG - chr5_4:50419245-50419264 Msa0856060:intron 50.0%
TTGCCCGGTTACTGTAGTCA+AGG - chr5_4:50419260-50419279 Msa0856060:intron 50.0%
TGCCCGGTTACTGTAGTCAA+GGG - chr5_4:50419261-50419280 Msa0856060:intron 50.0%
TATACGACTGTGGTCTGGAC+CGG - chr5_4:50418225-50418244 Msa0856060:CDS 50.0%
CTTGAACCAGGCAATGGTAG+TGG - chr5_4:50418974-50418993 Msa0856060:intron 50.0%
AGGTGTATTGGGGAGATCCA+AGG - chr5_4:50419000-50419019 Msa0856060:intron 50.0%
GGTGTATTGGGGAGATCCAA+GGG - chr5_4:50419001-50419020 Msa0856060:intron 50.0%
AGGCAGTGTTAGCAAGCATG+TGG - chr5_4:50419224-50419243 Msa0856060:intron 50.0%
GGTGACAAATGCTTCTTGCC+CGG - chr5_4:50419245-50419264 Msa0856060:intron 50.0%
TTGCCCGGTTACTGTAGTCA+AGG - chr5_4:50419260-50419279 Msa0856060:intron 50.0%
TGCCCGGTTACTGTAGTCAA+GGG - chr5_4:50419261-50419280 Msa0856060:intron 50.0%
CTAGTGTGATCAGCTGAGGG+AGG + chr5_4:50418067-50418086 None:intergenic 55.0%
ATACGACTGTGGTCTGGACC+GGG - chr5_4:50418226-50418245 Msa0856060:CDS 55.0%
CGCAGCCCAGATTGCATTCA+TGG - chr5_4:50418442-50418461 Msa0856060:CDS 55.0%
!! TGCAGCTTTGGTGCCTCTAG+AGG - chr5_4:50418581-50418600 Msa0856060:intron 55.0%
CCTTTGCCACTACCATTGCC+TGG + chr5_4:50418983-50419002 None:intergenic 55.0%
!! CCAGGCAATGGTAGTGGCAA+AGG - chr5_4:50418980-50418999 Msa0856060:intron 55.0%
CTAGTGTGATCAGCTGAGGG+AGG + chr5_4:50418067-50418086 None:intergenic 55.0%
ATACGACTGTGGTCTGGACC+GGG - chr5_4:50418226-50418245 Msa0856060:CDS 55.0%
CGCAGCCCAGATTGCATTCA+TGG - chr5_4:50418442-50418461 Msa0856060:CDS 55.0%
!! TGCAGCTTTGGTGCCTCTAG+AGG - chr5_4:50418581-50418600 Msa0856060:intron 55.0%
CCTTTGCCACTACCATTGCC+TGG + chr5_4:50418983-50419002 None:intergenic 55.0%
!! CCAGGCAATGGTAGTGGCAA+AGG - chr5_4:50418980-50418999 Msa0856060:intron 55.0%
TGTGATCAGCTGAGGGAGGC+TGG + chr5_4:50418063-50418082 None:intergenic 60.0%
GAAACCCTGATGTTGCGGCC+CGG + chr5_4:50418247-50418266 None:intergenic 60.0%
TGTGATCAGCTGAGGGAGGC+TGG + chr5_4:50418063-50418082 None:intergenic 60.0%
GAAACCCTGATGTTGCGGCC+CGG + chr5_4:50418247-50418266 None:intergenic 60.0%
TGGACCGGGCCGCAACATCA+GGG - chr5_4:50418240-50418259 Msa0856060:CDS 65.0%
TGGACCGGGCCGCAACATCA+GGG - chr5_4:50418240-50418259 Msa0856060:CDS 65.0%
CTGGACCGGGCCGCAACATC+AGG - chr5_4:50418239-50418258 Msa0856060:CDS 70.0%
CTGGACCGGGCCGCAACATC+AGG - chr5_4:50418239-50418258 Msa0856060:CDS 70.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 50417899 50419604 50417899 ID=Msa0856060;Name=Msa0856060
chr5_4 mRNA 50417899 50419604 50417899 ID=Msa0856060-mRNA-1;Parent=Msa0856060;Name=Msa0856060-mRNA-1;_AED=0.09;_eAED=0.09;_QI=42|1|1|1|1|1|4|293|166
chr5_4 exon 50417899 50418288 50417899 ID=Msa0856060-mRNA-1:exon:15811;Parent=Msa0856060-mRNA-1
chr5_4 exon 50418401 50418519 50418401 ID=Msa0856060-mRNA-1:exon:15810;Parent=Msa0856060-mRNA-1
chr5_4 exon 50418808 50418917 50418808 ID=Msa0856060-mRNA-1:exon:15809;Parent=Msa0856060-mRNA-1
chr5_4 exon 50419388 50419604 50419388 ID=Msa0856060-mRNA-1:exon:15808;Parent=Msa0856060-mRNA-1
chr5_4 five_prime_UTR 50419563 50419604 50419563 ID=Msa0856060-mRNA-1:five_prime_utr;Parent=Msa0856060-mRNA-1
chr5_4 CDS 50419388 50419562 50419388 ID=Msa0856060-mRNA-1:cds;Parent=Msa0856060-mRNA-1
chr5_4 CDS 50418808 50418917 50418808 ID=Msa0856060-mRNA-1:cds;Parent=Msa0856060-mRNA-1
chr5_4 CDS 50418401 50418519 50418401 ID=Msa0856060-mRNA-1:cds;Parent=Msa0856060-mRNA-1
chr5_4 CDS 50418192 50418288 50418192 ID=Msa0856060-mRNA-1:cds;Parent=Msa0856060-mRNA-1
chr5_4 three_prime_UTR 50417899 50418191 50417899 ID=Msa0856060-mRNA-1:three_prime_utr;Parent=Msa0856060-mRNA-1
Gene Sequence

>Msa0856060

ATGGCAAAAGCACGCATAGTTGGAGTAGCTATGGACTTCTCTCCTACCAGCAAATTAGCACTTAGATGGACTGTTGATAATCTGATCAACAAAAATGATCAAATCATCATGATCAATGTCCAGCCTCCCTCAGCTGATCACACTAGAAAGGAGCTTTTTGAAGACACTGGATCACCTTTGGTGCCTCTAGAGGAATTAAGGGAGATAAACTTTACAAAGCAATATGGAATTGCTAAGGATCCTGAAGTCATAGATATTCTTGAAACTGCCTCAAAAACCAAAGGGGCAATGGTAGTGGCAAAGGTGTATTGGGGAGATCCAAGGGAGAAATTGTGCAATGCTGTGCAAGATCTTCATCTGGACTCATTGGTTATTGGAAGCAGGGGTTTAGGTTCCATTAAAAGTGTGTTGTTAGGCAGTGTTAGCAAGCATGTGGTGACAAATGCTTCTTGCCCGGTTACTGTAGTCAAGGGAATGCAACCTTCCAAGTCTAGGCATTGA
Protein sequence

>Msa0856060

MAKARIVGVAMDFSPTSKLALRWTVDNLINKNDQIIMINVQPPSADHTRKELFEDTGSPLVPLEELREINFTKQYGIAKDPEVIDILETASKTKGAMVVAKVYWGDPREKLCNAVQDLHLDSLVIGSRGLGSIKSVLLGSVSKHVVTNASCPVTVVKGMQPSKSRH*