Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0738190 | XP_003616568.1 | 98.893 | 271 | 3 | 0 | 1 | 271 | 1 | 271 | 0.0 | 564 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0738190 | sp|Q8S9H7|DIV_ANTMA | 58.879 | 214 | 76 | 5 | 3 | 206 | 1 | 212 | 1.25e-79 | 245 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0738190 | G7KBX0 | 98.893 | 271 | 3 | 0 | 1 | 271 | 1 | 271 | 0.0 | 564 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0738190 | TF | MYB |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0738190 | Msa0817630 | 0.925827 | 1.081900e-90 | -8.615850e-47 |
| Msa0738190 | Msa0858070 | 0.907007 | 8.065893e-81 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0738190 | MtrunA17_Chr5g0436501 | 98.893 | 271 | 3 | 0 | 1 | 271 | 1 | 271 | 0.0 | 564 |
| Msa0738190 | MtrunA17_Chr7g0241761 | 62.150 | 214 | 72 | 2 | 3 | 209 | 11 | 222 | 7.45e-90 | 269 |
| Msa0738190 | MtrunA17_Chr5g0429521 | 61.386 | 202 | 71 | 3 | 2 | 198 | 4 | 203 | 7.75e-85 | 256 |
| Msa0738190 | MtrunA17_Chr3g0104071 | 64.796 | 196 | 56 | 4 | 16 | 200 | 27 | 220 | 1.74e-83 | 253 |
| Msa0738190 | MtrunA17_Chr1g0162081 | 51.832 | 191 | 76 | 4 | 25 | 202 | 10 | 197 | 2.34e-56 | 182 |
| Msa0738190 | MtrunA17_Chr1g0210471 | 49.733 | 187 | 80 | 4 | 25 | 204 | 22 | 201 | 3.72e-55 | 178 |
| Msa0738190 | MtrunA17_Chr4g0062741 | 42.381 | 210 | 81 | 5 | 27 | 201 | 8 | 212 | 2.47e-47 | 159 |
| Msa0738190 | MtrunA17_Chr8g0365011 | 43.229 | 192 | 79 | 4 | 25 | 202 | 6 | 181 | 1.04e-38 | 137 |
| Msa0738190 | MtrunA17_Chr2g0331031 | 61.111 | 90 | 33 | 1 | 120 | 209 | 104 | 191 | 1.30e-29 | 114 |
| Msa0738190 | MtrunA17_Chr6g0488471 | 69.863 | 73 | 20 | 1 | 119 | 191 | 91 | 161 | 6.77e-29 | 111 |
| Msa0738190 | MtrunA17_Chr4g0055931 | 64.198 | 81 | 27 | 1 | 120 | 200 | 90 | 168 | 6.12e-28 | 109 |
| Msa0738190 | MtrunA17_Chr8g0389241 | 60.714 | 84 | 30 | 1 | 116 | 199 | 96 | 176 | 5.88e-27 | 105 |
| Msa0738190 | MtrunA17_Chr5g0415191 | 60.000 | 90 | 34 | 1 | 112 | 201 | 89 | 176 | 6.52e-26 | 103 |
| Msa0738190 | MtrunA17_Chr4g0059701 | 71.186 | 59 | 17 | 0 | 113 | 171 | 72 | 130 | 1.02e-24 | 100 |
| Msa0738190 | MtrunA17_Chr5g0409841 | 49.524 | 105 | 49 | 1 | 92 | 192 | 55 | 159 | 7.93e-24 | 96.3 |
| Msa0738190 | MtrunA17_Chr8g0364741 | 40.984 | 122 | 66 | 2 | 25 | 142 | 8 | 127 | 1.20e-23 | 93.2 |
| Msa0738190 | MtrunA17_Chr5g0440741 | 53.933 | 89 | 39 | 2 | 105 | 192 | 81 | 168 | 4.36e-23 | 95.5 |
| Msa0738190 | MtrunA17_Chr3g0135671 | 51.429 | 105 | 44 | 4 | 90 | 191 | 58 | 158 | 8.35e-23 | 93.6 |
| Msa0738190 | MtrunA17_Chr5g0440731 | 58.904 | 73 | 29 | 1 | 121 | 192 | 101 | 173 | 9.01e-23 | 94.7 |
| Msa0738190 | MtrunA17_Chr1g0155181 | 55.882 | 68 | 30 | 0 | 22 | 89 | 2 | 69 | 4.17e-21 | 85.1 |
| Msa0738190 | MtrunA17_Chr3g0092621 | 57.971 | 69 | 27 | 1 | 125 | 193 | 84 | 150 | 1.65e-20 | 86.3 |
| Msa0738190 | MtrunA17_Chr3g0141601 | 53.846 | 65 | 30 | 0 | 25 | 89 | 5 | 69 | 1.49e-19 | 81.3 |
| Msa0738190 | MtrunA17_Chr6g0449111 | 52.941 | 68 | 32 | 0 | 22 | 89 | 5 | 72 | 8.35e-19 | 78.6 |
| Msa0738190 | MtrunA17_Chr5g0409831 | 58.904 | 73 | 29 | 1 | 121 | 192 | 73 | 145 | 1.16e-18 | 82.4 |
| Msa0738190 | MtrunA17_Chr5g0432921 | 33.000 | 200 | 119 | 7 | 26 | 218 | 20 | 211 | 1.85e-18 | 81.6 |
| Msa0738190 | MtrunA17_Chr3g0113121 | 40.764 | 157 | 79 | 7 | 48 | 196 | 21 | 171 | 2.74e-18 | 81.3 |
| Msa0738190 | MtrunA17_Chr7g0255841 | 52.941 | 68 | 32 | 0 | 30 | 97 | 15 | 82 | 4.31e-18 | 77.4 |
| Msa0738190 | MtrunA17_Chr8g0364731 | 60.294 | 68 | 27 | 0 | 135 | 202 | 11 | 78 | 6.43e-18 | 79.3 |
| Msa0738190 | MtrunA17_Chr6g0452791 | 57.627 | 59 | 25 | 0 | 125 | 183 | 80 | 138 | 9.87e-18 | 78.6 |
| Msa0738190 | MtrunA17_Chr5g0404101 | 45.333 | 75 | 41 | 0 | 15 | 89 | 7 | 81 | 1.31e-17 | 76.3 |
| Msa0738190 | MtrunA17_Chr3g0144631 | 52.381 | 63 | 30 | 0 | 27 | 89 | 16 | 78 | 1.55e-17 | 75.5 |
| Msa0738190 | MtrunA17_Chr3g0081631 | 47.945 | 73 | 37 | 1 | 18 | 89 | 1 | 73 | 9.85e-17 | 73.6 |
| Msa0738190 | MtrunA17_Chr5g0432941 | 30.000 | 200 | 124 | 5 | 26 | 218 | 21 | 211 | 1.08e-16 | 77.0 |
| Msa0738190 | MtrunA17_Chr8g0373131 | 49.231 | 65 | 32 | 1 | 25 | 89 | 3 | 66 | 2.15e-15 | 69.3 |
| Msa0738190 | MtrunA17_Chr8g0373111 | 49.231 | 65 | 32 | 1 | 25 | 89 | 3 | 66 | 2.26e-15 | 69.3 |
| Msa0738190 | MtrunA17_Chr8g0373121 | 49.231 | 65 | 32 | 1 | 25 | 89 | 3 | 66 | 2.26e-15 | 69.3 |
| Msa0738190 | MtrunA17_Chr8g0373101 | 50.820 | 61 | 29 | 1 | 27 | 87 | 5 | 64 | 4.73e-14 | 65.9 |
| Msa0738190 | MtrunA17_Chr5g0404301 | 50.820 | 61 | 29 | 1 | 27 | 87 | 4 | 63 | 2.41e-13 | 63.5 |
| Msa0738190 | MtrunA17_Chr1g0192061 | 55.714 | 70 | 29 | 2 | 127 | 195 | 63 | 131 | 2.59e-13 | 67.0 |
| Msa0738190 | MtrunA17_Chr3g0141641 | 38.806 | 67 | 41 | 0 | 27 | 93 | 3 | 69 | 3.80e-13 | 63.9 |
| Msa0738190 | MtrunA17_Chr8g0370121 | 50.000 | 50 | 25 | 0 | 122 | 171 | 97 | 146 | 7.62e-12 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0738190 | AT2G38090.1 | 59.716 | 211 | 69 | 2 | 3 | 199 | 5 | 213 | 4.35e-86 | 259 |
| Msa0738190 | AT3G11280.2 | 59.910 | 222 | 70 | 6 | 3 | 212 | 1 | 215 | 1.22e-80 | 244 |
| Msa0738190 | AT3G11280.1 | 59.910 | 222 | 70 | 6 | 3 | 212 | 1 | 215 | 1.22e-80 | 244 |
| Msa0738190 | AT5G05790.2 | 56.828 | 227 | 88 | 6 | 3 | 222 | 4 | 227 | 1.46e-79 | 241 |
| Msa0738190 | AT5G05790.1 | 56.828 | 227 | 88 | 6 | 3 | 222 | 4 | 227 | 1.46e-79 | 241 |
| Msa0738190 | AT5G58900.1 | 58.019 | 212 | 70 | 4 | 16 | 210 | 15 | 224 | 4.16e-77 | 236 |
| Msa0738190 | AT5G01200.1 | 54.067 | 209 | 73 | 6 | 6 | 196 | 12 | 215 | 4.20e-67 | 209 |
| Msa0738190 | AT5G08520.1 | 51.087 | 184 | 80 | 2 | 27 | 202 | 12 | 193 | 9.41e-54 | 176 |
| Msa0738190 | AT1G49010.1 | 45.050 | 202 | 81 | 4 | 21 | 194 | 2 | 201 | 1.82e-49 | 166 |
| Msa0738190 | AT5G04760.1 | 43.333 | 210 | 89 | 7 | 25 | 231 | 4 | 186 | 3.04e-46 | 154 |
| Msa0738190 | AT5G23650.1 | 42.654 | 211 | 89 | 5 | 20 | 215 | 6 | 199 | 5.68e-44 | 152 |
| Msa0738190 | AT3G10580.2 | 38.164 | 207 | 98 | 7 | 27 | 231 | 8 | 186 | 2.26e-35 | 127 |
| Msa0738190 | AT3G10580.1 | 38.164 | 207 | 98 | 7 | 27 | 231 | 8 | 186 | 4.64e-35 | 127 |
| Msa0738190 | AT4G09450.1 | 42.515 | 167 | 80 | 4 | 26 | 192 | 6 | 156 | 4.42e-32 | 117 |
| Msa0738190 | AT5G56840.1 | 63.415 | 82 | 29 | 1 | 120 | 201 | 86 | 166 | 1.14e-27 | 107 |
| Msa0738190 | AT5G47390.1 | 66.250 | 80 | 25 | 1 | 112 | 191 | 83 | 160 | 1.29e-27 | 109 |
| Msa0738190 | AT5G61620.1 | 52.941 | 102 | 46 | 1 | 91 | 192 | 74 | 173 | 7.23e-27 | 106 |
| Msa0738190 | AT1G19000.2 | 51.327 | 113 | 48 | 3 | 112 | 219 | 89 | 199 | 3.44e-25 | 101 |
| Msa0738190 | AT1G19000.1 | 51.327 | 113 | 48 | 3 | 112 | 219 | 89 | 199 | 3.44e-25 | 101 |
| Msa0738190 | AT1G70000.1 | 72.131 | 61 | 17 | 0 | 112 | 172 | 85 | 145 | 6.16e-25 | 100 |
| Msa0738190 | AT1G70000.2 | 72.131 | 61 | 17 | 0 | 112 | 172 | 85 | 145 | 6.16e-25 | 100 |
| Msa0738190 | AT3G16350.1 | 55.238 | 105 | 42 | 2 | 116 | 217 | 125 | 227 | 6.26e-25 | 102 |
| Msa0738190 | AT3G10590.1 | 40.196 | 204 | 96 | 12 | 27 | 216 | 7 | 198 | 5.20e-23 | 94.0 |
| Msa0738190 | AT1G74840.2 | 75.000 | 52 | 13 | 0 | 120 | 171 | 91 | 142 | 5.69e-22 | 92.0 |
| Msa0738190 | AT1G74840.1 | 75.000 | 52 | 13 | 0 | 120 | 171 | 91 | 142 | 8.13e-22 | 92.0 |
| Msa0738190 | AT1G75250.2 | 55.556 | 63 | 28 | 0 | 27 | 89 | 12 | 74 | 6.93e-18 | 77.0 |
| Msa0738190 | AT1G75250.1 | 55.556 | 63 | 28 | 0 | 27 | 89 | 12 | 74 | 7.07e-18 | 78.2 |
| Msa0738190 | AT4G39250.1 | 48.485 | 66 | 34 | 0 | 27 | 92 | 14 | 79 | 1.59e-17 | 76.3 |
| Msa0738190 | AT2G21650.1 | 47.761 | 67 | 35 | 0 | 27 | 93 | 14 | 80 | 3.88e-16 | 72.8 |
| Msa0738190 | AT2G18328.1 | 54.098 | 61 | 27 | 1 | 29 | 89 | 14 | 73 | 4.18e-14 | 66.6 |
| Msa0738190 | AT1G19510.1 | 51.724 | 58 | 28 | 0 | 32 | 89 | 17 | 74 | 4.77e-13 | 64.3 |
| Msa0738190 | AT3G10595.1 | 29.054 | 148 | 81 | 5 | 24 | 171 | 3 | 126 | 2.65e-12 | 64.3 |
Find 83 sgRNAs with CRISPR-Local
Find 124 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATTATTGCAGTTTGCTTTA+AGG | 0.090941 | 5_1:-55891907 | None:intergenic |
| CTCAATCCTTTAACAGAATT+TGG | 0.092721 | 5_1:+55891931 | Msa0738190:three_prime_UTR |
| AATAAGTTCTTATCTAAATT+TGG | 0.139369 | 5_1:+55892150 | Msa0738190:three_prime_UTR |
| TCCACTCCAAATTCTGTTAA+AGG | 0.150880 | 5_1:-55891937 | None:intergenic |
| GTAGTAGTATTTGTGATTTA+AGG | 0.198334 | 5_1:+55890328 | Msa0738190:five_prime_UTR |
| GTCTCCTATGCAGCAGAAAA+TGG | 0.222210 | 5_1:+55891685 | Msa0738190:CDS |
| TATGCTAAGCTGAAAGTTTC+GGG | 0.251091 | 5_1:+55891782 | Msa0738190:CDS |
| CTGATGGGACTTCTAAAGTA+TGG | 0.266546 | 5_1:+55891455 | Msa0738190:CDS |
| ACCAGGAATCGGAACATTTC+CGG | 0.268577 | 5_1:-55890593 | None:intergenic |
| TGCAGTTTGCTTTAAGGTTC+AGG | 0.269442 | 5_1:-55891901 | None:intergenic |
| GCCGGAAATGTTCCGATTCC+TGG | 0.317971 | 5_1:+55890592 | Msa0738190:CDS |
| TTATGCTAAGCTGAAAGTTT+CGG | 0.326332 | 5_1:+55891781 | Msa0738190:CDS |
| CTTCTCTACTCCATTCTGTT+TGG | 0.332735 | 5_1:-55890420 | None:intergenic |
| TAGTGATCATGTCTATCTTT+TGG | 0.334038 | 5_1:+55892005 | Msa0738190:three_prime_UTR |
| AGGAGACACATTGACTAATA+AGG | 0.335368 | 5_1:-55891669 | None:intergenic |
| ACATAAAATAAGGAGAATAA+AGG | 0.337256 | 5_1:-55890366 | None:intergenic |
| TGTGATTTAAGGTATGGATT+TGG | 0.345223 | 5_1:+55890339 | Msa0738190:exon |
| AGGGTGGTGTATAATATTCT+TGG | 0.349156 | 5_1:+55890051 | Msa0738190:five_prime_UTR |
| TGATGTGATTAAGCAGTATA+GGG | 0.364968 | 5_1:+55890540 | Msa0738190:CDS |
| AGATTCTGTATGATACTAAA+AGG | 0.365142 | 5_1:+55890159 | Msa0738190:five_prime_UTR |
| AAAGTAGAAGTGAGTTCAAA+AGG | 0.365448 | 5_1:-55889740 | None:intergenic |
| AGAAGATATGAAAATATTAC+AGG | 0.384170 | 5_1:-55889971 | None:intergenic |
| AGAAGGCATGTGACTGTTAG+AGG | 0.384719 | 5_1:+55890676 | Msa0738190:CDS |
| AAGATTGCTGAGATGATTCC+TGG | 0.391655 | 5_1:+55890505 | Msa0738190:CDS |
| ATATTCAACCTTTCTTTAAG+AGG | 0.395823 | 5_1:+55890031 | Msa0738190:five_prime_UTR |
| TTGATGTGATTAAGCAGTAT+AGG | 0.399334 | 5_1:+55890539 | Msa0738190:CDS |
| AGTATTTGTGATTTAAGGTA+TGG | 0.403756 | 5_1:+55890333 | Msa0738190:exon |
| TTTGTATCCAACATAAAATA+AGG | 0.405866 | 5_1:-55890376 | None:intergenic |
| ATAAGGCAAAAGGTTTCTTC+TGG | 0.415949 | 5_1:+55891563 | Msa0738190:CDS |
| GCAATCTTAAGCCATCTATC+TGG | 0.417904 | 5_1:-55890490 | None:intergenic |
| TAAAGTATGGCAAAGGCGAC+TGG | 0.439380 | 5_1:+55891468 | Msa0738190:CDS |
| ATCACAAATACTACTACAAA+AGG | 0.439751 | 5_1:-55890322 | None:intergenic |
| CATAAAATAAGGAGAATAAA+GGG | 0.445828 | 5_1:-55890365 | None:intergenic |
| GATTAAGCAGTATAGGGAAT+TGG | 0.449715 | 5_1:+55890546 | Msa0738190:CDS |
| GCATGACTTGCTACTTGTGT+TGG | 0.455619 | 5_1:-55891527 | None:intergenic |
| TGCTCTTGCTATATATGATA+AGG | 0.457525 | 5_1:+55890462 | Msa0738190:CDS |
| TGAGACACAGCATGTCTGAA+TGG | 0.469648 | 5_1:-55892054 | None:intergenic |
| TACACCACCCTCTTAAAGAA+AGG | 0.470360 | 5_1:-55890039 | None:intergenic |
| CACTTTGTCGTTTCTTCATG+AGG | 0.474646 | 5_1:-55889835 | None:intergenic |
| ATAAGGATACACCAGATAGA+TGG | 0.475384 | 5_1:+55890479 | Msa0738190:CDS |
| ATGCACAAAAGTACTACATA+AGG | 0.480834 | 5_1:+55891546 | Msa0738190:CDS |
| AGAGGAAGAAAGGTGTTCCT+TGG | 0.489408 | 5_1:+55890710 | Msa0738190:CDS |
| AAATACTACTACAAAAGGAT+TGG | 0.490445 | 5_1:-55890317 | None:intergenic |
| CAAGAGCAAGAGGGTGAAAG+AGG | 0.493861 | 5_1:-55889942 | None:intergenic |
| GAGACACAGCATGTCTGAAT+GGG | 0.499137 | 5_1:-55892053 | None:intergenic |
| AAGTGAGTTCAAAAGGAAGA+AGG | 0.507219 | 5_1:-55889733 | None:intergenic |
| AAAGTACTACATAAGGCAAA+AGG | 0.510073 | 5_1:+55891553 | Msa0738190:CDS |
| TATTCAACCTTTCTTTAAGA+GGG | 0.510102 | 5_1:+55890032 | Msa0738190:five_prime_UTR |
| GAAGCAACAATAAAAGCAAA+TGG | 0.511111 | 5_1:-55889813 | None:intergenic |
| AATAAGGACTTGTTATCTGA+TGG | 0.512003 | 5_1:-55891653 | None:intergenic |
| GTTGATGACATGAAGCTACT+TGG | 0.515565 | 5_1:+55891867 | Msa0738190:three_prime_UTR |
| GAGGCAAGATAACCAGGAAT+CGG | 0.520347 | 5_1:-55890604 | None:intergenic |
| TGATGGATCACCACAAGGGC+AGG | 0.524353 | 5_1:+55891730 | Msa0738190:CDS |
| TCAGCTTAGCATAAGCCTCA+TGG | 0.535139 | 5_1:-55891772 | None:intergenic |
| TCTCCTATGCAGCAGAAAAT+GGG | 0.535185 | 5_1:+55891686 | Msa0738190:CDS |
| AGTACTAGCCACTACAATGA+TGG | 0.540673 | 5_1:+55891713 | Msa0738190:CDS |
| TCTGATCATGAGAGGAAGAA+AGG | 0.544690 | 5_1:+55890700 | Msa0738190:CDS |
| TCAACCTTTCTTTAAGAGGG+TGG | 0.551667 | 5_1:+55890035 | Msa0738190:five_prime_UTR |
| AAAGAAGAGGCAAGATAACC+AGG | 0.552310 | 5_1:-55890610 | None:intergenic |
| AAGAGAGTTACCAAACAGAA+TGG | 0.556624 | 5_1:+55890410 | Msa0738190:CDS |
| TCCTTTAACAGAATTTGGAG+TGG | 0.565291 | 5_1:+55891936 | Msa0738190:three_prime_UTR |
| AGGCAAAAGGTTTCTTCTGG+AGG | 0.582472 | 5_1:+55891566 | Msa0738190:CDS |
| AAGAGCAAGAGGGTGAAAGA+GGG | 0.582574 | 5_1:-55889941 | None:intergenic |
| ACTATGATGGAAACAGAAGA+AGG | 0.582942 | 5_1:+55890659 | Msa0738190:CDS |
| TCATAGAGATCCTGCCCTTG+TGG | 0.585420 | 5_1:-55891740 | None:intergenic |
| CTTGGACTGAAGAGGAACAT+AGG | 0.585629 | 5_1:+55890728 | Msa0738190:CDS |
| CTATGTTCCTCTTCAGTCCA+AGG | 0.586053 | 5_1:-55890727 | None:intergenic |
| CTATGCAGCAGAAAATGGGT+TGG | 0.587490 | 5_1:+55891690 | Msa0738190:CDS |
| AGGTGTTCCTTGGACTGAAG+AGG | 0.592742 | 5_1:+55890720 | Msa0738190:CDS |
| CAGGAACATTCAAGAGCAAG+AGG | 0.595900 | 5_1:-55889952 | None:intergenic |
| GTTGTGATATCATGAATGCT+TGG | 0.600782 | 5_1:-55891605 | None:intergenic |
| CTATGACTGCTCTTTCCATG+AGG | 0.601527 | 5_1:+55891757 | Msa0738190:CDS |
| GCTTCTAGAGACTTCAACAA+AGG | 0.612158 | 5_1:+55891815 | Msa0738190:CDS |
| GATGTGAGTGAAATTGAGGC+CGG | 0.613302 | 5_1:+55890574 | Msa0738190:CDS |
| TACAATGATGGATCACCACA+AGG | 0.622159 | 5_1:+55891725 | Msa0738190:CDS |
| AACTTCAAAGGTGAAAGAAG+AGG | 0.626616 | 5_1:-55890623 | None:intergenic |
| ACATCAATAACAGTCTTACC+AGG | 0.630015 | 5_1:-55890523 | None:intergenic |
| TTAGAGGTTCTGATCATGAG+AGG | 0.633143 | 5_1:+55890692 | Msa0738190:CDS |
| GTGGTGATCCATCATTGTAG+TGG | 0.633599 | 5_1:-55891721 | None:intergenic |
| AGAAGATGTGAGTGAAATTG+AGG | 0.635385 | 5_1:+55890570 | Msa0738190:CDS |
| GGACTTCTAAAGTATGGCAA+AGG | 0.636983 | 5_1:+55891461 | Msa0738190:CDS |
| AGGAACATTCAAGAGCAAGA+GGG | 0.693394 | 5_1:-55889951 | None:intergenic |
| ACAATGATGGATCACCACAA+GGG | 0.720384 | 5_1:+55891726 | Msa0738190:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGTAAAAGAATAAAAAGAAA+AGG | - | chr5_1:55890785-55890804 | None:intergenic | 15.0% |
| !! | AAATAAAAAACCTAAAATCT+AGG | - | chr5_1:55891084-55891103 | None:intergenic | 15.0% |
| !!! | ATAACAAGTAACTTTTAAAA+GGG | - | chr5_1:55891258-55891277 | None:intergenic | 15.0% |
| !!! | AATAACAAGTAACTTTTAAA+AGG | - | chr5_1:55891259-55891278 | None:intergenic | 15.0% |
| !!! | AATAACAAGTAACTTTTAAA+CGG | - | chr5_1:55891259-55891278 | None:intergenic | 15.0% |
| !! | AATAAGTTCTTATCTAAATT+TGG | + | chr5_1:55892150-55892169 | Msa0738190:three_prime_UTR | 15.0% |
| !! | AGAAGATATGAAAATATTAC+AGG | - | chr5_1:55889974-55889993 | None:intergenic | 20.0% |
| !!! | TTCATATCTTCTATCTTTTT+TGG | + | chr5_1:55889982-55890001 | Msa0738190:five_prime_UTR | 20.0% |
| !! | CATAAAATAAGGAGAATAAA+GGG | - | chr5_1:55890368-55890387 | None:intergenic | 20.0% |
| !! | ACATAAAATAAGGAGAATAA+AGG | - | chr5_1:55890369-55890388 | None:intergenic | 20.0% |
| !!! | TTATTCTCCTTATTTTATGT+TGG | + | chr5_1:55890369-55890388 | Msa0738190:CDS | 20.0% |
| !! | TTTGTATCCAACATAAAATA+AGG | - | chr5_1:55890379-55890398 | None:intergenic | 20.0% |
| !!! | ATTTTATGTTGGATACAAAT+TGG | + | chr5_1:55890380-55890399 | Msa0738190:CDS | 20.0% |
| !!! | TTTTTCTAGTAGTATACTAT+AGG | - | chr5_1:55890852-55890871 | None:intergenic | 20.0% |
| !!! | ATTTCATTTTCCTAGATTTT+AGG | + | chr5_1:55891071-55891090 | Msa0738190:intron | 20.0% |
| !!! | TAACAAGTAACTTTTAAAAG+GGG | - | chr5_1:55891257-55891276 | None:intergenic | 20.0% |
| !! | ATTGAACTTGATTATTAGTT+TGG | + | chr5_1:55891276-55891295 | Msa0738190:intron | 20.0% |
| !!! | ATAACAAGTAACTTTTAAAC+GGG | - | chr5_1:55891387-55891406 | None:intergenic | 20.0% |
| ! | ATCAAACAAAGGAAAAAAGA+TGG | - | chr5_1:55889873-55889892 | None:intergenic | 25.0% |
| !! | GATTCTTTTCAATCAAACAA+AGG | - | chr5_1:55889884-55889903 | None:intergenic | 25.0% |
| !!! | ATCTATCTTTTTTTCGCTAT+TGG | + | chr5_1:55889901-55889920 | Msa0738190:five_prime_UTR | 25.0% |
| ! | ATATTCAACCTTTCTTTAAG+AGG | + | chr5_1:55890031-55890050 | Msa0738190:five_prime_UTR | 25.0% |
| !! | TATTCAACCTTTCTTTAAGA+GGG | + | chr5_1:55890032-55890051 | Msa0738190:five_prime_UTR | 25.0% |
| !!! | TTTGAGACTATTGATTTTGA+TGG | - | chr5_1:55890083-55890102 | None:intergenic | 25.0% |
| ! | AGATTCTGTATGATACTAAA+AGG | + | chr5_1:55890159-55890178 | Msa0738190:five_prime_UTR | 25.0% |
| !!! | CTATAATTTCCTCTTTTTTG+TGG | + | chr5_1:55890274-55890293 | Msa0738190:five_prime_UTR | 25.0% |
| ! | CATAAAAAACCACAAAAAAG+AGG | - | chr5_1:55890286-55890305 | None:intergenic | 25.0% |
| ! | AAATACTACTACAAAAGGAT+TGG | - | chr5_1:55890320-55890339 | None:intergenic | 25.0% |
| ! | ATCACAAATACTACTACAAA+AGG | - | chr5_1:55890325-55890344 | None:intergenic | 25.0% |
| ! | GTAGTAGTATTTGTGATTTA+AGG | + | chr5_1:55890328-55890347 | Msa0738190:five_prime_UTR | 25.0% |
| !! | AGTATTTGTGATTTAAGGTA+TGG | + | chr5_1:55890333-55890352 | Msa0738190:exon | 25.0% |
| ! | AGAAAAATTGAAGTAAGACA+CGG | + | chr5_1:55890865-55890884 | Msa0738190:intron | 25.0% |
| ! | AGACACGGAAAAAATAAAAT+CGG | + | chr5_1:55890880-55890899 | Msa0738190:intron | 25.0% |
| ! | TAGCTAATGAATCATATTCA+TGG | + | chr5_1:55891020-55891039 | Msa0738190:intron | 25.0% |
| !!! | ATGTTAAATTGAGTTGGTTT+AGG | + | chr5_1:55891348-55891367 | Msa0738190:intron | 25.0% |
| !! | TAACAAGTAACTTTTAAACG+GGG | - | chr5_1:55891386-55891405 | None:intergenic | 25.0% |
| AAAGTAGAAGTGAGTTCAAA+AGG | - | chr5_1:55889743-55889762 | None:intergenic | 30.0% | |
| GAAGCAACAATAAAAGCAAA+TGG | - | chr5_1:55889816-55889835 | None:intergenic | 30.0% | |
| ! | TGTGATTTAAGGTATGGATT+TGG | + | chr5_1:55890339-55890358 | Msa0738190:exon | 30.0% |
| ! | TGCTCTTGCTATATATGATA+AGG | + | chr5_1:55890462-55890481 | Msa0738190:CDS | 30.0% |
| TTGATGTGATTAAGCAGTAT+AGG | + | chr5_1:55890539-55890558 | Msa0738190:CDS | 30.0% | |
| TGATGTGATTAAGCAGTATA+GGG | + | chr5_1:55890540-55890559 | Msa0738190:CDS | 30.0% | |
| !! | TTGTTTTTCGACAACTTCAA+AGG | - | chr5_1:55890638-55890657 | None:intergenic | 30.0% |
| GTCGAAAAACAAAACTATGA+TGG | + | chr5_1:55890646-55890665 | Msa0738190:CDS | 30.0% | |
| ! | TTCTTTTACTCCTTTCTCTA+TGG | + | chr5_1:55890795-55890814 | Msa0738190:intron | 30.0% |
| GAAAAAACTTCCATAGAGAA+AGG | - | chr5_1:55890808-55890827 | None:intergenic | 30.0% | |
| !!! | GGAAGTTTTTTCTTAGATTG+TGG | + | chr5_1:55890816-55890835 | Msa0738190:intron | 30.0% |
| !! | GTAACTTTTAAAAGGGGTAA+AGG | - | chr5_1:55891251-55891270 | None:intergenic | 30.0% |
| ATCGACATGTTAAATTGAGT+TGG | + | chr5_1:55891342-55891361 | Msa0738190:intron | 30.0% | |
| ATTTACTCTTTGTGCATGAT+TGG | + | chr5_1:55891410-55891429 | Msa0738190:intron | 30.0% | |
| !! | ATGCACAAAAGTACTACATA+AGG | + | chr5_1:55891546-55891565 | Msa0738190:CDS | 30.0% |
| ! | AAAGTACTACATAAGGCAAA+AGG | + | chr5_1:55891553-55891572 | Msa0738190:CDS | 30.0% |
| !! | AATAAGGACTTGTTATCTGA+TGG | - | chr5_1:55891656-55891675 | None:intergenic | 30.0% |
| TTATGCTAAGCTGAAAGTTT+CGG | + | chr5_1:55891781-55891800 | Msa0738190:CDS | 30.0% | |
| ! | CATTATTGCAGTTTGCTTTA+AGG | - | chr5_1:55891910-55891929 | None:intergenic | 30.0% |
| CTCAATCCTTTAACAGAATT+TGG | + | chr5_1:55891931-55891950 | Msa0738190:three_prime_UTR | 30.0% | |
| ! | TAGTGATCATGTCTATCTTT+TGG | + | chr5_1:55892005-55892024 | Msa0738190:three_prime_UTR | 30.0% |
| AAGTGAGTTCAAAAGGAAGA+AGG | - | chr5_1:55889736-55889755 | None:intergenic | 35.0% | |
| AGGGTGGTGTATAATATTCT+TGG | + | chr5_1:55890051-55890070 | Msa0738190:five_prime_UTR | 35.0% | |
| AAGAGAGTTACCAAACAGAA+TGG | + | chr5_1:55890410-55890429 | Msa0738190:CDS | 35.0% | |
| ATAAGGATACACCAGATAGA+TGG | + | chr5_1:55890479-55890498 | Msa0738190:CDS | 35.0% | |
| ACATCAATAACAGTCTTACC+AGG | - | chr5_1:55890526-55890545 | None:intergenic | 35.0% | |
| GATTAAGCAGTATAGGGAAT+TGG | + | chr5_1:55890546-55890565 | Msa0738190:CDS | 35.0% | |
| AGAAGATGTGAGTGAAATTG+AGG | + | chr5_1:55890570-55890589 | Msa0738190:CDS | 35.0% | |
| AACTTCAAAGGTGAAAGAAG+AGG | - | chr5_1:55890626-55890645 | None:intergenic | 35.0% | |
| ! | ACTATGATGGAAACAGAAGA+AGG | + | chr5_1:55890659-55890678 | Msa0738190:CDS | 35.0% |
| GAGTCTGTAACACTTAATGA+TGG | + | chr5_1:55890914-55890933 | Msa0738190:intron | 35.0% | |
| ! | GTAACTTTTAAACGGGGTAA+AGG | - | chr5_1:55891380-55891399 | None:intergenic | 35.0% |
| TTTGTGCATGATTGGAATCA+CGG | + | chr5_1:55891418-55891437 | Msa0738190:intron | 35.0% | |
| ATAAGGCAAAAGGTTTCTTC+TGG | + | chr5_1:55891563-55891582 | Msa0738190:CDS | 35.0% | |
| GTTGTGATATCATGAATGCT+TGG | - | chr5_1:55891608-55891627 | None:intergenic | 35.0% | |
| ! | AGGAGACACATTGACTAATA+AGG | - | chr5_1:55891672-55891691 | None:intergenic | 35.0% |
| TATGCTAAGCTGAAAGTTTC+GGG | + | chr5_1:55891782-55891801 | Msa0738190:CDS | 35.0% | |
| !! | TTCAACAAAGGAGCTGTTTT+TGG | + | chr5_1:55891827-55891846 | Msa0738190:CDS | 35.0% |
| TCCTTTAACAGAATTTGGAG+TGG | + | chr5_1:55891936-55891955 | Msa0738190:three_prime_UTR | 35.0% | |
| TCCACTCCAAATTCTGTTAA+AGG | - | chr5_1:55891940-55891959 | None:intergenic | 35.0% | |
| ! | CACTTTGTCGTTTCTTCATG+AGG | - | chr5_1:55889838-55889857 | None:intergenic | 40.0% |
| AGGAACATTCAAGAGCAAGA+GGG | - | chr5_1:55889954-55889973 | None:intergenic | 40.0% | |
| ! | TCAACCTTTCTTTAAGAGGG+TGG | + | chr5_1:55890035-55890054 | Msa0738190:five_prime_UTR | 40.0% |
| TACACCACCCTCTTAAAGAA+AGG | - | chr5_1:55890042-55890061 | None:intergenic | 40.0% | |
| CTTCTCTACTCCATTCTGTT+TGG | - | chr5_1:55890423-55890442 | None:intergenic | 40.0% | |
| GCAATCTTAAGCCATCTATC+TGG | - | chr5_1:55890493-55890512 | None:intergenic | 40.0% | |
| AAGATTGCTGAGATGATTCC+TGG | + | chr5_1:55890505-55890524 | Msa0738190:CDS | 40.0% | |
| AAAGAAGAGGCAAGATAACC+AGG | - | chr5_1:55890613-55890632 | None:intergenic | 40.0% | |
| ! | TTAGAGGTTCTGATCATGAG+AGG | + | chr5_1:55890692-55890711 | Msa0738190:CDS | 40.0% |
| TCTGATCATGAGAGGAAGAA+AGG | + | chr5_1:55890700-55890719 | Msa0738190:CDS | 40.0% | |
| GTAAAGGCAAACTCCAATTG+AGG | - | chr5_1:55891235-55891254 | None:intergenic | 40.0% | |
| ! | GCATACAGACGTTTTCTGAT+GGG | + | chr5_1:55891440-55891459 | Msa0738190:intron | 40.0% |
| !! | CTGATGGGACTTCTAAAGTA+TGG | + | chr5_1:55891455-55891474 | Msa0738190:CDS | 40.0% |
| !! | GGACTTCTAAAGTATGGCAA+AGG | + | chr5_1:55891461-55891480 | Msa0738190:CDS | 40.0% |
| TCTCCTATGCAGCAGAAAAT+GGG | + | chr5_1:55891686-55891705 | Msa0738190:CDS | 40.0% | |
| AGTACTAGCCACTACAATGA+TGG | + | chr5_1:55891713-55891732 | Msa0738190:CDS | 40.0% | |
| !! | TACAATGATGGATCACCACA+AGG | + | chr5_1:55891725-55891744 | Msa0738190:CDS | 40.0% |
| !! | ACAATGATGGATCACCACAA+GGG | + | chr5_1:55891726-55891745 | Msa0738190:CDS | 40.0% |
| ! | GCTTCTAGAGACTTCAACAA+AGG | + | chr5_1:55891815-55891834 | Msa0738190:CDS | 40.0% |
| !! | GTTGATGACATGAAGCTACT+TGG | + | chr5_1:55891867-55891886 | Msa0738190:three_prime_UTR | 40.0% |
| !! | TGCAGTTTGCTTTAAGGTTC+AGG | - | chr5_1:55891904-55891923 | None:intergenic | 40.0% |
| AAGAGCAAGAGGGTGAAAGA+GGG | - | chr5_1:55889944-55889963 | None:intergenic | 45.0% | |
| CAGGAACATTCAAGAGCAAG+AGG | - | chr5_1:55889955-55889974 | None:intergenic | 45.0% | |
| GATGTGAGTGAAATTGAGGC+CGG | + | chr5_1:55890574-55890593 | Msa0738190:CDS | 45.0% | |
| ACCAGGAATCGGAACATTTC+CGG | - | chr5_1:55890596-55890615 | None:intergenic | 45.0% | |
| GAGGCAAGATAACCAGGAAT+CGG | - | chr5_1:55890607-55890626 | None:intergenic | 45.0% | |
| ! | AGAAGGCATGTGACTGTTAG+AGG | + | chr5_1:55890676-55890695 | Msa0738190:CDS | 45.0% |
| !! | AGAGGAAGAAAGGTGTTCCT+TGG | + | chr5_1:55890710-55890729 | Msa0738190:CDS | 45.0% |
| CTATGTTCCTCTTCAGTCCA+AGG | - | chr5_1:55890730-55890749 | None:intergenic | 45.0% | |
| CTTGGACTGAAGAGGAACAT+AGG | + | chr5_1:55890728-55890747 | Msa0738190:CDS | 45.0% | |
| GCAGATAATGCAGCCTCAAT+TGG | + | chr5_1:55891219-55891238 | Msa0738190:intron | 45.0% | |
| ! | GGCATACAGACGTTTTCTGA+TGG | + | chr5_1:55891439-55891458 | Msa0738190:intron | 45.0% |
| TAAAGTATGGCAAAGGCGAC+TGG | + | chr5_1:55891468-55891487 | Msa0738190:CDS | 45.0% | |
| !! | GCATGACTTGCTACTTGTGT+TGG | - | chr5_1:55891530-55891549 | None:intergenic | 45.0% |
| AGGCAAAAGGTTTCTTCTGG+AGG | + | chr5_1:55891566-55891585 | Msa0738190:CDS | 45.0% | |
| GTCTCCTATGCAGCAGAAAA+TGG | + | chr5_1:55891685-55891704 | Msa0738190:CDS | 45.0% | |
| ! | CAACCCATTTTCTGCTGCAT+AGG | - | chr5_1:55891692-55891711 | None:intergenic | 45.0% |
| CTATGCAGCAGAAAATGGGT+TGG | + | chr5_1:55891690-55891709 | Msa0738190:CDS | 45.0% | |
| GTGGTGATCCATCATTGTAG+TGG | - | chr5_1:55891724-55891743 | None:intergenic | 45.0% | |
| CTATGACTGCTCTTTCCATG+AGG | + | chr5_1:55891757-55891776 | Msa0738190:CDS | 45.0% | |
| TCAGCTTAGCATAAGCCTCA+TGG | - | chr5_1:55891775-55891794 | None:intergenic | 45.0% | |
| GAGACACAGCATGTCTGAAT+GGG | - | chr5_1:55892056-55892075 | None:intergenic | 45.0% | |
| TGAGACACAGCATGTCTGAA+TGG | - | chr5_1:55892057-55892076 | None:intergenic | 45.0% | |
| CAAGAGCAAGAGGGTGAAAG+AGG | - | chr5_1:55889945-55889964 | None:intergenic | 50.0% | |
| AGGTGTTCCTTGGACTGAAG+AGG | + | chr5_1:55890720-55890739 | Msa0738190:CDS | 50.0% | |
| TCATAGAGATCCTGCCCTTG+TGG | - | chr5_1:55891743-55891762 | None:intergenic | 50.0% | |
| GCCGGAAATGTTCCGATTCC+TGG | + | chr5_1:55890592-55890611 | Msa0738190:CDS | 55.0% | |
| ! | TGATGGATCACCACAAGGGC+AGG | + | chr5_1:55891730-55891749 | Msa0738190:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_1 | gene | 55889686 | 55892183 | 55889686 | ID=Msa0738190;Name=Msa0738190 |
| chr5_1 | mRNA | 55889686 | 55892183 | 55889686 | ID=Msa0738190-mRNA-1;Parent=Msa0738190;Name=Msa0738190-mRNA-1;_AED=0.00;_eAED=0.00;_QI=666|1|1|1|1|1|2|318|271 |
| chr5_1 | exon | 55889686 | 55890749 | 55889686 | ID=Msa0738190-mRNA-1:exon:15803;Parent=Msa0738190-mRNA-1 |
| chr5_1 | exon | 55891448 | 55892183 | 55891448 | ID=Msa0738190-mRNA-1:exon:15804;Parent=Msa0738190-mRNA-1 |
| chr5_1 | five_prime_UTR | 55889686 | 55890351 | 55889686 | ID=Msa0738190-mRNA-1:five_prime_utr;Parent=Msa0738190-mRNA-1 |
| chr5_1 | CDS | 55890352 | 55890749 | 55890352 | ID=Msa0738190-mRNA-1:cds;Parent=Msa0738190-mRNA-1 |
| chr5_1 | CDS | 55891448 | 55891865 | 55891448 | ID=Msa0738190-mRNA-1:cds;Parent=Msa0738190-mRNA-1 |
| chr5_1 | three_prime_UTR | 55891866 | 55892183 | 55891866 | ID=Msa0738190-mRNA-1:three_prime_utr;Parent=Msa0738190-mRNA-1 |
| Gene Sequence |
| Protein sequence |
