Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0858070 | XP_003616568.1 | 98.893 | 271 | 3 | 0 | 1 | 271 | 1 | 271 | 0.0 | 564 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0858070 | sp|Q8S9H7|DIV_ANTMA | 58.879 | 214 | 76 | 5 | 3 | 206 | 1 | 212 | 1.25e-79 | 245 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0858070 | G7KBX0 | 98.893 | 271 | 3 | 0 | 1 | 271 | 1 | 271 | 0.0 | 564 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0858070 | TF | MYB |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0738190 | Msa0858070 | 0.907007 | 8.065893e-81 | -8.615850e-47 |
| Msa0769300 | Msa0858070 | 0.806339 | 8.632015e-50 | -8.615850e-47 |
| Msa0817630 | Msa0858070 | 0.898664 | 4.250460e-77 | -8.615850e-47 |
| Msa0537950 | Msa0858070 | 0.803729 | 3.034030e-49 | -8.615850e-47 |
| Msa0538190 | Msa0858070 | 0.821187 | 4.612542e-53 | -8.615850e-47 |
| Msa0556230 | Msa0858070 | -0.805762 | 1.141704e-49 | -8.615850e-47 |
| Msa0858070 | Msa1408110 | 0.819550 | 1.095025e-52 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0858070 | MtrunA17_Chr5g0436501 | 98.893 | 271 | 3 | 0 | 1 | 271 | 1 | 271 | 0.0 | 564 |
| Msa0858070 | MtrunA17_Chr7g0241761 | 62.150 | 214 | 72 | 2 | 3 | 209 | 11 | 222 | 7.45e-90 | 269 |
| Msa0858070 | MtrunA17_Chr5g0429521 | 61.386 | 202 | 71 | 3 | 2 | 198 | 4 | 203 | 7.75e-85 | 256 |
| Msa0858070 | MtrunA17_Chr3g0104071 | 64.796 | 196 | 56 | 4 | 16 | 200 | 27 | 220 | 1.74e-83 | 253 |
| Msa0858070 | MtrunA17_Chr1g0162081 | 51.832 | 191 | 76 | 4 | 25 | 202 | 10 | 197 | 2.34e-56 | 182 |
| Msa0858070 | MtrunA17_Chr1g0210471 | 49.733 | 187 | 80 | 4 | 25 | 204 | 22 | 201 | 3.72e-55 | 178 |
| Msa0858070 | MtrunA17_Chr4g0062741 | 42.381 | 210 | 81 | 5 | 27 | 201 | 8 | 212 | 2.47e-47 | 159 |
| Msa0858070 | MtrunA17_Chr8g0365011 | 43.229 | 192 | 79 | 4 | 25 | 202 | 6 | 181 | 1.04e-38 | 137 |
| Msa0858070 | MtrunA17_Chr2g0331031 | 61.111 | 90 | 33 | 1 | 120 | 209 | 104 | 191 | 1.30e-29 | 114 |
| Msa0858070 | MtrunA17_Chr6g0488471 | 69.863 | 73 | 20 | 1 | 119 | 191 | 91 | 161 | 6.77e-29 | 111 |
| Msa0858070 | MtrunA17_Chr4g0055931 | 64.198 | 81 | 27 | 1 | 120 | 200 | 90 | 168 | 6.12e-28 | 109 |
| Msa0858070 | MtrunA17_Chr8g0389241 | 60.714 | 84 | 30 | 1 | 116 | 199 | 96 | 176 | 5.88e-27 | 105 |
| Msa0858070 | MtrunA17_Chr5g0415191 | 60.000 | 90 | 34 | 1 | 112 | 201 | 89 | 176 | 6.52e-26 | 103 |
| Msa0858070 | MtrunA17_Chr4g0059701 | 71.186 | 59 | 17 | 0 | 113 | 171 | 72 | 130 | 1.02e-24 | 100 |
| Msa0858070 | MtrunA17_Chr5g0409841 | 49.524 | 105 | 49 | 1 | 92 | 192 | 55 | 159 | 7.93e-24 | 96.3 |
| Msa0858070 | MtrunA17_Chr8g0364741 | 40.984 | 122 | 66 | 2 | 25 | 142 | 8 | 127 | 1.20e-23 | 93.2 |
| Msa0858070 | MtrunA17_Chr5g0440741 | 53.933 | 89 | 39 | 2 | 105 | 192 | 81 | 168 | 4.36e-23 | 95.5 |
| Msa0858070 | MtrunA17_Chr3g0135671 | 51.429 | 105 | 44 | 4 | 90 | 191 | 58 | 158 | 8.35e-23 | 93.6 |
| Msa0858070 | MtrunA17_Chr5g0440731 | 58.904 | 73 | 29 | 1 | 121 | 192 | 101 | 173 | 9.01e-23 | 94.7 |
| Msa0858070 | MtrunA17_Chr1g0155181 | 55.882 | 68 | 30 | 0 | 22 | 89 | 2 | 69 | 4.17e-21 | 85.1 |
| Msa0858070 | MtrunA17_Chr3g0092621 | 57.971 | 69 | 27 | 1 | 125 | 193 | 84 | 150 | 1.65e-20 | 86.3 |
| Msa0858070 | MtrunA17_Chr3g0141601 | 53.846 | 65 | 30 | 0 | 25 | 89 | 5 | 69 | 1.49e-19 | 81.3 |
| Msa0858070 | MtrunA17_Chr6g0449111 | 52.941 | 68 | 32 | 0 | 22 | 89 | 5 | 72 | 8.35e-19 | 78.6 |
| Msa0858070 | MtrunA17_Chr5g0409831 | 58.904 | 73 | 29 | 1 | 121 | 192 | 73 | 145 | 1.16e-18 | 82.4 |
| Msa0858070 | MtrunA17_Chr5g0432921 | 33.000 | 200 | 119 | 7 | 26 | 218 | 20 | 211 | 1.85e-18 | 81.6 |
| Msa0858070 | MtrunA17_Chr3g0113121 | 40.764 | 157 | 79 | 7 | 48 | 196 | 21 | 171 | 2.74e-18 | 81.3 |
| Msa0858070 | MtrunA17_Chr7g0255841 | 52.941 | 68 | 32 | 0 | 30 | 97 | 15 | 82 | 4.31e-18 | 77.4 |
| Msa0858070 | MtrunA17_Chr8g0364731 | 60.294 | 68 | 27 | 0 | 135 | 202 | 11 | 78 | 6.43e-18 | 79.3 |
| Msa0858070 | MtrunA17_Chr6g0452791 | 57.627 | 59 | 25 | 0 | 125 | 183 | 80 | 138 | 9.87e-18 | 78.6 |
| Msa0858070 | MtrunA17_Chr5g0404101 | 45.333 | 75 | 41 | 0 | 15 | 89 | 7 | 81 | 1.31e-17 | 76.3 |
| Msa0858070 | MtrunA17_Chr3g0144631 | 52.381 | 63 | 30 | 0 | 27 | 89 | 16 | 78 | 1.55e-17 | 75.5 |
| Msa0858070 | MtrunA17_Chr3g0081631 | 47.945 | 73 | 37 | 1 | 18 | 89 | 1 | 73 | 9.85e-17 | 73.6 |
| Msa0858070 | MtrunA17_Chr5g0432941 | 30.000 | 200 | 124 | 5 | 26 | 218 | 21 | 211 | 1.08e-16 | 77.0 |
| Msa0858070 | MtrunA17_Chr8g0373131 | 49.231 | 65 | 32 | 1 | 25 | 89 | 3 | 66 | 2.15e-15 | 69.3 |
| Msa0858070 | MtrunA17_Chr8g0373111 | 49.231 | 65 | 32 | 1 | 25 | 89 | 3 | 66 | 2.26e-15 | 69.3 |
| Msa0858070 | MtrunA17_Chr8g0373121 | 49.231 | 65 | 32 | 1 | 25 | 89 | 3 | 66 | 2.26e-15 | 69.3 |
| Msa0858070 | MtrunA17_Chr8g0373101 | 50.820 | 61 | 29 | 1 | 27 | 87 | 5 | 64 | 4.73e-14 | 65.9 |
| Msa0858070 | MtrunA17_Chr5g0404301 | 50.820 | 61 | 29 | 1 | 27 | 87 | 4 | 63 | 2.41e-13 | 63.5 |
| Msa0858070 | MtrunA17_Chr1g0192061 | 55.714 | 70 | 29 | 2 | 127 | 195 | 63 | 131 | 2.59e-13 | 67.0 |
| Msa0858070 | MtrunA17_Chr3g0141641 | 38.806 | 67 | 41 | 0 | 27 | 93 | 3 | 69 | 3.80e-13 | 63.9 |
| Msa0858070 | MtrunA17_Chr8g0370121 | 50.000 | 50 | 25 | 0 | 122 | 171 | 97 | 146 | 7.62e-12 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0858070 | AT2G38090.1 | 59.716 | 211 | 69 | 2 | 3 | 199 | 5 | 213 | 4.35e-86 | 259 |
| Msa0858070 | AT3G11280.2 | 59.910 | 222 | 70 | 6 | 3 | 212 | 1 | 215 | 1.22e-80 | 244 |
| Msa0858070 | AT3G11280.1 | 59.910 | 222 | 70 | 6 | 3 | 212 | 1 | 215 | 1.22e-80 | 244 |
| Msa0858070 | AT5G05790.2 | 56.828 | 227 | 88 | 6 | 3 | 222 | 4 | 227 | 1.46e-79 | 241 |
| Msa0858070 | AT5G05790.1 | 56.828 | 227 | 88 | 6 | 3 | 222 | 4 | 227 | 1.46e-79 | 241 |
| Msa0858070 | AT5G58900.1 | 58.019 | 212 | 70 | 4 | 16 | 210 | 15 | 224 | 4.16e-77 | 236 |
| Msa0858070 | AT5G01200.1 | 54.067 | 209 | 73 | 6 | 6 | 196 | 12 | 215 | 4.20e-67 | 209 |
| Msa0858070 | AT5G08520.1 | 51.087 | 184 | 80 | 2 | 27 | 202 | 12 | 193 | 9.41e-54 | 176 |
| Msa0858070 | AT1G49010.1 | 45.050 | 202 | 81 | 4 | 21 | 194 | 2 | 201 | 1.82e-49 | 166 |
| Msa0858070 | AT5G04760.1 | 43.333 | 210 | 89 | 7 | 25 | 231 | 4 | 186 | 3.04e-46 | 154 |
| Msa0858070 | AT5G23650.1 | 42.654 | 211 | 89 | 5 | 20 | 215 | 6 | 199 | 5.68e-44 | 152 |
| Msa0858070 | AT3G10580.2 | 38.164 | 207 | 98 | 7 | 27 | 231 | 8 | 186 | 2.26e-35 | 127 |
| Msa0858070 | AT3G10580.1 | 38.164 | 207 | 98 | 7 | 27 | 231 | 8 | 186 | 4.64e-35 | 127 |
| Msa0858070 | AT4G09450.1 | 42.515 | 167 | 80 | 4 | 26 | 192 | 6 | 156 | 4.42e-32 | 117 |
| Msa0858070 | AT5G56840.1 | 63.415 | 82 | 29 | 1 | 120 | 201 | 86 | 166 | 1.14e-27 | 107 |
| Msa0858070 | AT5G47390.1 | 66.250 | 80 | 25 | 1 | 112 | 191 | 83 | 160 | 1.29e-27 | 109 |
| Msa0858070 | AT5G61620.1 | 52.941 | 102 | 46 | 1 | 91 | 192 | 74 | 173 | 7.23e-27 | 106 |
| Msa0858070 | AT1G19000.2 | 51.327 | 113 | 48 | 3 | 112 | 219 | 89 | 199 | 3.44e-25 | 101 |
| Msa0858070 | AT1G19000.1 | 51.327 | 113 | 48 | 3 | 112 | 219 | 89 | 199 | 3.44e-25 | 101 |
| Msa0858070 | AT1G70000.1 | 72.131 | 61 | 17 | 0 | 112 | 172 | 85 | 145 | 6.16e-25 | 100 |
| Msa0858070 | AT1G70000.2 | 72.131 | 61 | 17 | 0 | 112 | 172 | 85 | 145 | 6.16e-25 | 100 |
| Msa0858070 | AT3G16350.1 | 55.238 | 105 | 42 | 2 | 116 | 217 | 125 | 227 | 6.26e-25 | 102 |
| Msa0858070 | AT3G10590.1 | 40.196 | 204 | 96 | 12 | 27 | 216 | 7 | 198 | 5.20e-23 | 94.0 |
| Msa0858070 | AT1G74840.2 | 75.000 | 52 | 13 | 0 | 120 | 171 | 91 | 142 | 5.69e-22 | 92.0 |
| Msa0858070 | AT1G74840.1 | 75.000 | 52 | 13 | 0 | 120 | 171 | 91 | 142 | 8.13e-22 | 92.0 |
| Msa0858070 | AT1G75250.2 | 55.556 | 63 | 28 | 0 | 27 | 89 | 12 | 74 | 6.93e-18 | 77.0 |
| Msa0858070 | AT1G75250.1 | 55.556 | 63 | 28 | 0 | 27 | 89 | 12 | 74 | 7.07e-18 | 78.2 |
| Msa0858070 | AT4G39250.1 | 48.485 | 66 | 34 | 0 | 27 | 92 | 14 | 79 | 1.59e-17 | 76.3 |
| Msa0858070 | AT2G21650.1 | 47.761 | 67 | 35 | 0 | 27 | 93 | 14 | 80 | 3.88e-16 | 72.8 |
| Msa0858070 | AT2G18328.1 | 54.098 | 61 | 27 | 1 | 29 | 89 | 14 | 73 | 4.18e-14 | 66.6 |
| Msa0858070 | AT1G19510.1 | 51.724 | 58 | 28 | 0 | 32 | 89 | 17 | 74 | 4.77e-13 | 64.3 |
| Msa0858070 | AT3G10595.1 | 29.054 | 148 | 81 | 5 | 24 | 171 | 3 | 126 | 2.65e-12 | 64.3 |
Find 84 sgRNAs with CRISPR-Local
Find 126 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATTATTGCAGTTTGCTTTA+AGG | 0.090941 | 5_4:-55101071 | None:intergenic |
| CTCAATCCTTTAACAGAATT+TGG | 0.092721 | 5_4:+55101095 | Msa0858070:three_prime_UTR |
| AATAAGTTCTTATCTAAATT+TGG | 0.139369 | 5_4:+55101315 | Msa0858070:three_prime_UTR |
| TCCACTCCAAATTCTGTTAA+AGG | 0.150880 | 5_4:-55101101 | None:intergenic |
| GTAGTAGTATTTGTGATTTA+AGG | 0.198334 | 5_4:+55099479 | Msa0858070:five_prime_UTR |
| GTCTCCTATGCAGCAGAAAA+TGG | 0.222210 | 5_4:+55100849 | Msa0858070:CDS |
| TATGCTAAGCTGAAAGTTTC+GGG | 0.251091 | 5_4:+55100946 | Msa0858070:CDS |
| CTGATGGGACTTCTAAAGTA+TGG | 0.266546 | 5_4:+55100619 | Msa0858070:CDS |
| ACCAGGAATCGGAACATTTC+CGG | 0.268577 | 5_4:-55099744 | None:intergenic |
| TGCAGTTTGCTTTAAGGTTC+AGG | 0.269442 | 5_4:-55101065 | None:intergenic |
| AGAAGATATGAAAATATTAT+AGG | 0.300464 | 5_4:-55099122 | None:intergenic |
| GCCGGAAATGTTCCGATTCC+TGG | 0.317971 | 5_4:+55099743 | Msa0858070:CDS |
| TTATGCTAAGCTGAAAGTTT+CGG | 0.326332 | 5_4:+55100945 | Msa0858070:CDS |
| CTTCTCTACTCCATTCTGTT+TGG | 0.332735 | 5_4:-55099571 | None:intergenic |
| TAGTGATCATGTCTATCTTT+TGG | 0.334038 | 5_4:+55101170 | Msa0858070:three_prime_UTR |
| AGGAGACACATTGACTAATA+AGG | 0.335368 | 5_4:-55100833 | None:intergenic |
| ACATAAAATAAGGAGAATAA+AGG | 0.337256 | 5_4:-55099517 | None:intergenic |
| TGTGATTTAAGGTATGGATT+TGG | 0.345223 | 5_4:+55099490 | Msa0858070:exon |
| AGGGTGGTGTATAATATTCT+TGG | 0.349156 | 5_4:+55099202 | Msa0858070:five_prime_UTR |
| TGATGTGATTAAGCAGTATA+GGG | 0.364968 | 5_4:+55099691 | Msa0858070:CDS |
| AGATTCTGTATGATACTAAA+AGG | 0.365142 | 5_4:+55099310 | Msa0858070:five_prime_UTR |
| AAAGTAGAAGTGAGTTCAAA+AGG | 0.365448 | 5_4:-55098886 | None:intergenic |
| AGAAGGCATGTGACTGTTAG+AGG | 0.384719 | 5_4:+55099827 | Msa0858070:CDS |
| AAATACTACTACAAAAGGTT+TGG | 0.386778 | 5_4:-55099468 | None:intergenic |
| AAGATTGCTGAGATGATTCC+TGG | 0.391655 | 5_4:+55099656 | Msa0858070:CDS |
| TTCGTATCCAACATAAAATA+AGG | 0.394704 | 5_4:-55099527 | None:intergenic |
| ATATTCAACCTTTCTTTAAG+AGG | 0.395823 | 5_4:+55099182 | Msa0858070:five_prime_UTR |
| TTGATGTGATTAAGCAGTAT+AGG | 0.399334 | 5_4:+55099690 | Msa0858070:CDS |
| AGTATTTGTGATTTAAGGTA+TGG | 0.403756 | 5_4:+55099484 | Msa0858070:exon |
| ATCACAAATACTACTACAAA+AGG | 0.412501 | 5_4:-55099473 | None:intergenic |
| ATAAGGCAAAAGGTTTCTTC+TGG | 0.415949 | 5_4:+55100727 | Msa0858070:CDS |
| GCAATCTTAAGCCATCTATC+TGG | 0.417904 | 5_4:-55099641 | None:intergenic |
| TAAAGTATGGCAAAGGCGAC+TGG | 0.439380 | 5_4:+55100632 | Msa0858070:CDS |
| CATAAAATAAGGAGAATAAA+GGG | 0.445828 | 5_4:-55099516 | None:intergenic |
| GATTAAGCAGTATAGGGAAT+TGG | 0.449715 | 5_4:+55099697 | Msa0858070:CDS |
| TGCTCTTGCTATATATGATA+AGG | 0.457525 | 5_4:+55099613 | Msa0858070:CDS |
| TGAGACACAGCATGTCTGAA+TGG | 0.469648 | 5_4:-55101219 | None:intergenic |
| TACACCACCCTCTTAAAGAA+AGG | 0.470360 | 5_4:-55099190 | None:intergenic |
| CACTTTGTCGTTTCTTCATG+AGG | 0.474646 | 5_4:-55098981 | None:intergenic |
| ATAAGGATACACCAGATAGA+TGG | 0.475384 | 5_4:+55099630 | Msa0858070:CDS |
| ATGCACAAAAGTACTACATA+AGG | 0.480834 | 5_4:+55100710 | Msa0858070:CDS |
| AGAGGAAGAAAGGTGTTCCT+TGG | 0.489408 | 5_4:+55099861 | Msa0858070:CDS |
| CAAGAGCAAGAGGGTGAAAG+AGG | 0.493861 | 5_4:-55099093 | None:intergenic |
| GAGACACAGCATGTCTGAAT+GGG | 0.499137 | 5_4:-55101218 | None:intergenic |
| AAGTGAGTTCAAAAGGAAGA+AGG | 0.507219 | 5_4:-55098879 | None:intergenic |
| AAAGTACTACATAAGGCAAA+AGG | 0.510073 | 5_4:+55100717 | Msa0858070:CDS |
| TATTCAACCTTTCTTTAAGA+GGG | 0.510102 | 5_4:+55099183 | Msa0858070:five_prime_UTR |
| GAAGCAACAATAAAAGCAAA+TGG | 0.511111 | 5_4:-55098959 | None:intergenic |
| GCATGACTTGCCACTTGTGT+TGG | 0.511936 | 5_4:-55100691 | None:intergenic |
| AATAAGGACTTGTTATCTGA+TGG | 0.512003 | 5_4:-55100817 | None:intergenic |
| GTTGATGACATGAAGCTACT+TGG | 0.515565 | 5_4:+55101031 | Msa0858070:three_prime_UTR |
| GAGGCAAGATAACCAGGAAT+CGG | 0.520347 | 5_4:-55099755 | None:intergenic |
| TGATGGATCACCACAAGGGC+AGG | 0.524353 | 5_4:+55100894 | Msa0858070:CDS |
| TCAGCTTAGCATAAGCCTCA+TGG | 0.535139 | 5_4:-55100936 | None:intergenic |
| TCTCCTATGCAGCAGAAAAT+GGG | 0.535185 | 5_4:+55100850 | Msa0858070:CDS |
| AGTACTAGCCACTACAATGA+TGG | 0.540673 | 5_4:+55100877 | Msa0858070:CDS |
| TCTGATCATGAGAGGAAGAA+AGG | 0.544690 | 5_4:+55099851 | Msa0858070:CDS |
| TCAACCTTTCTTTAAGAGGG+TGG | 0.551667 | 5_4:+55099186 | Msa0858070:five_prime_UTR |
| AAAGAAGAGGCAAGATAACC+AGG | 0.552310 | 5_4:-55099761 | None:intergenic |
| AAGAGAGTTACCAAACAGAA+TGG | 0.556624 | 5_4:+55099561 | Msa0858070:CDS |
| TCCTTTAACAGAATTTGGAG+TGG | 0.565291 | 5_4:+55101100 | Msa0858070:three_prime_UTR |
| GACAAAGACTCCAACACAAG+TGG | 0.578965 | 5_4:+55100681 | Msa0858070:CDS |
| AGGCAAAAGGTTTCTTCTGG+AGG | 0.582472 | 5_4:+55100730 | Msa0858070:CDS |
| AAGAGCAAGAGGGTGAAAGA+GGG | 0.582574 | 5_4:-55099092 | None:intergenic |
| ACTATGATGGAAACAGAAGA+AGG | 0.582942 | 5_4:+55099810 | Msa0858070:CDS |
| TCATAGAGATCCTGCCCTTG+TGG | 0.585420 | 5_4:-55100904 | None:intergenic |
| CTTGGACTGAAGAGGAACAT+AGG | 0.585629 | 5_4:+55099879 | Msa0858070:CDS |
| CTATGTTCCTCTTCAGTCCA+AGG | 0.586053 | 5_4:-55099878 | None:intergenic |
| CTATGCAGCAGAAAATGGGT+TGG | 0.587490 | 5_4:+55100854 | Msa0858070:CDS |
| AGGTGTTCCTTGGACTGAAG+AGG | 0.592742 | 5_4:+55099871 | Msa0858070:CDS |
| TAGGAACATTCAAGAGCAAG+AGG | 0.598552 | 5_4:-55099103 | None:intergenic |
| GTTGTGATATCATGAATGCT+TGG | 0.600782 | 5_4:-55100769 | None:intergenic |
| CTATGACTGCTCTTTCCATG+AGG | 0.601527 | 5_4:+55100921 | Msa0858070:CDS |
| GCTTCTAGAGACTTCAACAA+AGG | 0.612158 | 5_4:+55100979 | Msa0858070:CDS |
| GATGTGAGTGAAATTGAGGC+CGG | 0.613302 | 5_4:+55099725 | Msa0858070:CDS |
| TACAATGATGGATCACCACA+AGG | 0.622159 | 5_4:+55100889 | Msa0858070:CDS |
| AACTTCAAAGGTGAAAGAAG+AGG | 0.626616 | 5_4:-55099774 | None:intergenic |
| ACATCAATAACAGTCTTACC+AGG | 0.630015 | 5_4:-55099674 | None:intergenic |
| TTAGAGGTTCTGATCATGAG+AGG | 0.633143 | 5_4:+55099843 | Msa0858070:CDS |
| GTGGTGATCCATCATTGTAG+TGG | 0.633599 | 5_4:-55100885 | None:intergenic |
| AGAAGATGTGAGTGAAATTG+AGG | 0.635385 | 5_4:+55099721 | Msa0858070:CDS |
| GGACTTCTAAAGTATGGCAA+AGG | 0.636983 | 5_4:+55100625 | Msa0858070:CDS |
| AGGAACATTCAAGAGCAAGA+GGG | 0.687281 | 5_4:-55099102 | None:intergenic |
| ACAATGATGGATCACCACAA+GGG | 0.720384 | 5_4:+55100890 | Msa0858070:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGAAGATATGAAAATATTAT+AGG | - | chr5_4:55099125-55099144 | None:intergenic | 15.0% |
| !! | AAATAAAAAACCTAAAATCT+AGG | - | chr5_4:55100248-55100267 | None:intergenic | 15.0% |
| !!! | ATAACAAGTAACTTTTAAAA+GGG | - | chr5_4:55100422-55100441 | None:intergenic | 15.0% |
| !!! | AATAACAAGTAACTTTTAAA+AGG | - | chr5_4:55100423-55100442 | None:intergenic | 15.0% |
| !! | AATAAGTTCTTATCTAAATT+TGG | + | chr5_4:55101315-55101334 | Msa0858070:three_prime_UTR | 15.0% |
| !!! | TATCTTTTTTTTCTCTCTAT+TGG | + | chr5_4:55099050-55099069 | Msa0858070:five_prime_UTR | 20.0% |
| !!! | TTCATATCTTCTATCTTTTT+TGG | + | chr5_4:55099133-55099152 | Msa0858070:five_prime_UTR | 20.0% |
| !! | CATAAAATAAGGAGAATAAA+GGG | - | chr5_4:55099519-55099538 | None:intergenic | 20.0% |
| !! | ACATAAAATAAGGAGAATAA+AGG | - | chr5_4:55099520-55099539 | None:intergenic | 20.0% |
| !!! | TTATTCTCCTTATTTTATGT+TGG | + | chr5_4:55099520-55099539 | Msa0858070:CDS | 20.0% |
| !! | AGTAAAAGAACAAAAAGAAA+AGG | - | chr5_4:55099936-55099955 | None:intergenic | 20.0% |
| !!! | ATTTCATTTTCCTAGATTTT+AGG | + | chr5_4:55100235-55100254 | Msa0858070:intron | 20.0% |
| !!! | TAACAAGTAACTTTTAAAAG+GGG | - | chr5_4:55100421-55100440 | None:intergenic | 20.0% |
| !! | ATTGAACTTGATTATTAGTT+TGG | + | chr5_4:55100440-55100459 | Msa0858070:intron | 20.0% |
| !!! | TAACAAGTAACTTTTAAACT+GGG | - | chr5_4:55100550-55100569 | None:intergenic | 20.0% |
| !!! | ATAACAAGTAACTTTTAAAC+TGG | - | chr5_4:55100551-55100570 | None:intergenic | 20.0% |
| ! | ATCAAACAAAGGAAAAAAGA+TGG | - | chr5_4:55099019-55099038 | None:intergenic | 25.0% |
| !! | GATTCTTTTCAATCAAACAA+AGG | - | chr5_4:55099030-55099049 | None:intergenic | 25.0% |
| ! | ATATTCAACCTTTCTTTAAG+AGG | + | chr5_4:55099182-55099201 | Msa0858070:five_prime_UTR | 25.0% |
| !! | TATTCAACCTTTCTTTAAGA+GGG | + | chr5_4:55099183-55099202 | Msa0858070:five_prime_UTR | 25.0% |
| !!! | TTTGAGACTATTGATTTTGA+TGG | - | chr5_4:55099234-55099253 | None:intergenic | 25.0% |
| ! | AGATTCTGTATGATACTAAA+AGG | + | chr5_4:55099310-55099329 | Msa0858070:five_prime_UTR | 25.0% |
| !!! | CTATAATTTCCTCTTTTTTG+TGG | + | chr5_4:55099425-55099444 | Msa0858070:five_prime_UTR | 25.0% |
| ! | CATAAAAAACCACAAAAAAG+AGG | - | chr5_4:55099437-55099456 | None:intergenic | 25.0% |
| ! | AAATACTACTACAAAAGGTT+TGG | - | chr5_4:55099471-55099490 | None:intergenic | 25.0% |
| ! | ATCACAAATACTACTACAAA+AGG | - | chr5_4:55099476-55099495 | None:intergenic | 25.0% |
| ! | GTAGTAGTATTTGTGATTTA+AGG | + | chr5_4:55099479-55099498 | Msa0858070:five_prime_UTR | 25.0% |
| !! | AGTATTTGTGATTTAAGGTA+TGG | + | chr5_4:55099484-55099503 | Msa0858070:exon | 25.0% |
| ! | TTCGTATCCAACATAAAATA+AGG | - | chr5_4:55099530-55099549 | None:intergenic | 25.0% |
| !! | ATTTTATGTTGGATACGAAT+TGG | + | chr5_4:55099531-55099550 | Msa0858070:CDS | 25.0% |
| !!! | TTTTTCTAGTAGTATACCAT+AGG | - | chr5_4:55100003-55100022 | None:intergenic | 25.0% |
| ! | AGAAAAATTGAAGTAAGACA+CGG | + | chr5_4:55100016-55100035 | Msa0858070:intron | 25.0% |
| ! | AGAGACGGAAAAAATAAAAT+CGG | + | chr5_4:55100044-55100063 | Msa0858070:intron | 25.0% |
| ! | TAGCTAATGAATCATATTCA+TGG | + | chr5_4:55100184-55100203 | Msa0858070:intron | 25.0% |
| !!! | ATGTTAAATTGAGTTGGTTT+AGG | + | chr5_4:55100512-55100531 | Msa0858070:intron | 25.0% |
| AAAGTAGAAGTGAGTTCAAA+AGG | - | chr5_4:55098889-55098908 | None:intergenic | 30.0% | |
| GAAGCAACAATAAAAGCAAA+TGG | - | chr5_4:55098962-55098981 | None:intergenic | 30.0% | |
| ! | TGTGATTTAAGGTATGGATT+TGG | + | chr5_4:55099490-55099509 | Msa0858070:exon | 30.0% |
| ! | TGCTCTTGCTATATATGATA+AGG | + | chr5_4:55099613-55099632 | Msa0858070:CDS | 30.0% |
| TTGATGTGATTAAGCAGTAT+AGG | + | chr5_4:55099690-55099709 | Msa0858070:CDS | 30.0% | |
| TGATGTGATTAAGCAGTATA+GGG | + | chr5_4:55099691-55099710 | Msa0858070:CDS | 30.0% | |
| !! | TTGTTTTTCGACAACTTCAA+AGG | - | chr5_4:55099789-55099808 | None:intergenic | 30.0% |
| GTCGAAAAACAAAACTATGA+TGG | + | chr5_4:55099797-55099816 | Msa0858070:CDS | 30.0% | |
| ! | TTCTTTTACTCCTTTCTCTA+TGG | + | chr5_4:55099946-55099965 | Msa0858070:intron | 30.0% |
| GAAAAAACTTCCATAGAGAA+AGG | - | chr5_4:55099959-55099978 | None:intergenic | 30.0% | |
| !!! | GGAAGTTTTTTCTTAGATTG+TGG | + | chr5_4:55099967-55099986 | Msa0858070:intron | 30.0% |
| !! | GTAACTTTTAAAAGGGGTAA+AGG | - | chr5_4:55100415-55100434 | None:intergenic | 30.0% |
| ATCGACATGTTAAATTGAGT+TGG | + | chr5_4:55100506-55100525 | Msa0858070:intron | 30.0% | |
| ! | GTAACTTTTAAACTGGGTAA+AGG | - | chr5_4:55100544-55100563 | None:intergenic | 30.0% |
| ATTTACTCTTTGTGCATGAT+TGG | + | chr5_4:55100574-55100593 | Msa0858070:intron | 30.0% | |
| !! | ATGCACAAAAGTACTACATA+AGG | + | chr5_4:55100710-55100729 | Msa0858070:CDS | 30.0% |
| ! | AAAGTACTACATAAGGCAAA+AGG | + | chr5_4:55100717-55100736 | Msa0858070:CDS | 30.0% |
| !! | AATAAGGACTTGTTATCTGA+TGG | - | chr5_4:55100820-55100839 | None:intergenic | 30.0% |
| TTATGCTAAGCTGAAAGTTT+CGG | + | chr5_4:55100945-55100964 | Msa0858070:CDS | 30.0% | |
| ! | CATTATTGCAGTTTGCTTTA+AGG | - | chr5_4:55101074-55101093 | None:intergenic | 30.0% |
| CTCAATCCTTTAACAGAATT+TGG | + | chr5_4:55101095-55101114 | Msa0858070:three_prime_UTR | 30.0% | |
| ! | TAGTGATCATGTCTATCTTT+TGG | + | chr5_4:55101170-55101189 | Msa0858070:three_prime_UTR | 30.0% |
| AAGTGAGTTCAAAAGGAAGA+AGG | - | chr5_4:55098882-55098901 | None:intergenic | 35.0% | |
| AGGGTGGTGTATAATATTCT+TGG | + | chr5_4:55099202-55099221 | Msa0858070:five_prime_UTR | 35.0% | |
| AAGAGAGTTACCAAACAGAA+TGG | + | chr5_4:55099561-55099580 | Msa0858070:CDS | 35.0% | |
| ATAAGGATACACCAGATAGA+TGG | + | chr5_4:55099630-55099649 | Msa0858070:CDS | 35.0% | |
| ACATCAATAACAGTCTTACC+AGG | - | chr5_4:55099677-55099696 | None:intergenic | 35.0% | |
| GATTAAGCAGTATAGGGAAT+TGG | + | chr5_4:55099697-55099716 | Msa0858070:CDS | 35.0% | |
| AGAAGATGTGAGTGAAATTG+AGG | + | chr5_4:55099721-55099740 | Msa0858070:CDS | 35.0% | |
| AACTTCAAAGGTGAAAGAAG+AGG | - | chr5_4:55099777-55099796 | None:intergenic | 35.0% | |
| ! | ACTATGATGGAAACAGAAGA+AGG | + | chr5_4:55099810-55099829 | Msa0858070:CDS | 35.0% |
| TTGTGGAGAAAGAAAACCTA+TGG | + | chr5_4:55099984-55100003 | Msa0858070:intron | 35.0% | |
| GAGTCTGTAACACTTAATGA+TGG | + | chr5_4:55100078-55100097 | Msa0858070:intron | 35.0% | |
| TTTGTGCATGATTGGAATCA+CGG | + | chr5_4:55100582-55100601 | Msa0858070:intron | 35.0% | |
| ATAAGGCAAAAGGTTTCTTC+TGG | + | chr5_4:55100727-55100746 | Msa0858070:CDS | 35.0% | |
| GTTGTGATATCATGAATGCT+TGG | - | chr5_4:55100772-55100791 | None:intergenic | 35.0% | |
| ! | AGGAGACACATTGACTAATA+AGG | - | chr5_4:55100836-55100855 | None:intergenic | 35.0% |
| TATGCTAAGCTGAAAGTTTC+GGG | + | chr5_4:55100946-55100965 | Msa0858070:CDS | 35.0% | |
| !! | TTCAACAAAGGAGCTGTTTT+TGG | + | chr5_4:55100991-55101010 | Msa0858070:CDS | 35.0% |
| TCCTTTAACAGAATTTGGAG+TGG | + | chr5_4:55101100-55101119 | Msa0858070:three_prime_UTR | 35.0% | |
| TCCACTCCAAATTCTGTTAA+AGG | - | chr5_4:55101104-55101123 | None:intergenic | 35.0% | |
| ! | CACTTTGTCGTTTCTTCATG+AGG | - | chr5_4:55098984-55099003 | None:intergenic | 40.0% |
| AGGAACATTCAAGAGCAAGA+GGG | - | chr5_4:55099105-55099124 | None:intergenic | 40.0% | |
| TAGGAACATTCAAGAGCAAG+AGG | - | chr5_4:55099106-55099125 | None:intergenic | 40.0% | |
| ! | TCAACCTTTCTTTAAGAGGG+TGG | + | chr5_4:55099186-55099205 | Msa0858070:five_prime_UTR | 40.0% |
| TACACCACCCTCTTAAAGAA+AGG | - | chr5_4:55099193-55099212 | None:intergenic | 40.0% | |
| CTTCTCTACTCCATTCTGTT+TGG | - | chr5_4:55099574-55099593 | None:intergenic | 40.0% | |
| GCAATCTTAAGCCATCTATC+TGG | - | chr5_4:55099644-55099663 | None:intergenic | 40.0% | |
| AAGATTGCTGAGATGATTCC+TGG | + | chr5_4:55099656-55099675 | Msa0858070:CDS | 40.0% | |
| AAAGAAGAGGCAAGATAACC+AGG | - | chr5_4:55099764-55099783 | None:intergenic | 40.0% | |
| ! | TTAGAGGTTCTGATCATGAG+AGG | + | chr5_4:55099843-55099862 | Msa0858070:CDS | 40.0% |
| TCTGATCATGAGAGGAAGAA+AGG | + | chr5_4:55099851-55099870 | Msa0858070:CDS | 40.0% | |
| GTAAAGGCAAACTCCAATTG+AGG | - | chr5_4:55100399-55100418 | None:intergenic | 40.0% | |
| ! | GCATACAGACGTTTTCTGAT+GGG | + | chr5_4:55100604-55100623 | Msa0858070:intron | 40.0% |
| !! | CTGATGGGACTTCTAAAGTA+TGG | + | chr5_4:55100619-55100638 | Msa0858070:CDS | 40.0% |
| !! | GGACTTCTAAAGTATGGCAA+AGG | + | chr5_4:55100625-55100644 | Msa0858070:CDS | 40.0% |
| TCTCCTATGCAGCAGAAAAT+GGG | + | chr5_4:55100850-55100869 | Msa0858070:CDS | 40.0% | |
| AGTACTAGCCACTACAATGA+TGG | + | chr5_4:55100877-55100896 | Msa0858070:CDS | 40.0% | |
| !! | TACAATGATGGATCACCACA+AGG | + | chr5_4:55100889-55100908 | Msa0858070:CDS | 40.0% |
| !! | ACAATGATGGATCACCACAA+GGG | + | chr5_4:55100890-55100909 | Msa0858070:CDS | 40.0% |
| ! | GCTTCTAGAGACTTCAACAA+AGG | + | chr5_4:55100979-55100998 | Msa0858070:CDS | 40.0% |
| !! | GTTGATGACATGAAGCTACT+TGG | + | chr5_4:55101031-55101050 | Msa0858070:three_prime_UTR | 40.0% |
| !! | TGCAGTTTGCTTTAAGGTTC+AGG | - | chr5_4:55101068-55101087 | None:intergenic | 40.0% |
| AAGAGCAAGAGGGTGAAAGA+GGG | - | chr5_4:55099095-55099114 | None:intergenic | 45.0% | |
| GATGTGAGTGAAATTGAGGC+CGG | + | chr5_4:55099725-55099744 | Msa0858070:CDS | 45.0% | |
| ACCAGGAATCGGAACATTTC+CGG | - | chr5_4:55099747-55099766 | None:intergenic | 45.0% | |
| GAGGCAAGATAACCAGGAAT+CGG | - | chr5_4:55099758-55099777 | None:intergenic | 45.0% | |
| ! | AGAAGGCATGTGACTGTTAG+AGG | + | chr5_4:55099827-55099846 | Msa0858070:CDS | 45.0% |
| !! | AGAGGAAGAAAGGTGTTCCT+TGG | + | chr5_4:55099861-55099880 | Msa0858070:CDS | 45.0% |
| CTATGTTCCTCTTCAGTCCA+AGG | - | chr5_4:55099881-55099900 | None:intergenic | 45.0% | |
| CTTGGACTGAAGAGGAACAT+AGG | + | chr5_4:55099879-55099898 | Msa0858070:CDS | 45.0% | |
| TAAGACACGGAAGCTAGAGA+CGG | + | chr5_4:55100029-55100048 | Msa0858070:intron | 45.0% | |
| GCAGATAATGCAGCCTCAAT+TGG | + | chr5_4:55100383-55100402 | Msa0858070:intron | 45.0% | |
| ! | GGCATACAGACGTTTTCTGA+TGG | + | chr5_4:55100603-55100622 | Msa0858070:intron | 45.0% |
| TAAAGTATGGCAAAGGCGAC+TGG | + | chr5_4:55100632-55100651 | Msa0858070:CDS | 45.0% | |
| GACAAAGACTCCAACACAAG+TGG | + | chr5_4:55100681-55100700 | Msa0858070:CDS | 45.0% | |
| AGGCAAAAGGTTTCTTCTGG+AGG | + | chr5_4:55100730-55100749 | Msa0858070:CDS | 45.0% | |
| GTCTCCTATGCAGCAGAAAA+TGG | + | chr5_4:55100849-55100868 | Msa0858070:CDS | 45.0% | |
| ! | CAACCCATTTTCTGCTGCAT+AGG | - | chr5_4:55100856-55100875 | None:intergenic | 45.0% |
| CTATGCAGCAGAAAATGGGT+TGG | + | chr5_4:55100854-55100873 | Msa0858070:CDS | 45.0% | |
| GTGGTGATCCATCATTGTAG+TGG | - | chr5_4:55100888-55100907 | None:intergenic | 45.0% | |
| CTATGACTGCTCTTTCCATG+AGG | + | chr5_4:55100921-55100940 | Msa0858070:CDS | 45.0% | |
| TCAGCTTAGCATAAGCCTCA+TGG | - | chr5_4:55100939-55100958 | None:intergenic | 45.0% | |
| GAGACACAGCATGTCTGAAT+GGG | - | chr5_4:55101221-55101240 | None:intergenic | 45.0% | |
| TGAGACACAGCATGTCTGAA+TGG | - | chr5_4:55101222-55101241 | None:intergenic | 45.0% | |
| CAAGAGCAAGAGGGTGAAAG+AGG | - | chr5_4:55099096-55099115 | None:intergenic | 50.0% | |
| AGGTGTTCCTTGGACTGAAG+AGG | + | chr5_4:55099871-55099890 | Msa0858070:CDS | 50.0% | |
| ! | GCATGACTTGCCACTTGTGT+TGG | - | chr5_4:55100694-55100713 | None:intergenic | 50.0% |
| TCATAGAGATCCTGCCCTTG+TGG | - | chr5_4:55100907-55100926 | None:intergenic | 50.0% | |
| GCCGGAAATGTTCCGATTCC+TGG | + | chr5_4:55099743-55099762 | Msa0858070:CDS | 55.0% | |
| ! | TGATGGATCACCACAAGGGC+AGG | + | chr5_4:55100894-55100913 | Msa0858070:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_4 | gene | 55098832 | 55101348 | 55098832 | ID=Msa0858070;Name=Msa0858070 |
| chr5_4 | mRNA | 55098832 | 55101348 | 55098832 | ID=Msa0858070-mRNA-1;Parent=Msa0858070;Name=Msa0858070-mRNA-1;_AED=0.00;_eAED=0.00;_QI=671|1|1|1|1|1|2|319|271 |
| chr5_4 | exon | 55098832 | 55099900 | 55098832 | ID=Msa0858070-mRNA-1:exon:16926;Parent=Msa0858070-mRNA-1 |
| chr5_4 | exon | 55100612 | 55101348 | 55100612 | ID=Msa0858070-mRNA-1:exon:16927;Parent=Msa0858070-mRNA-1 |
| chr5_4 | five_prime_UTR | 55098832 | 55099502 | 55098832 | ID=Msa0858070-mRNA-1:five_prime_utr;Parent=Msa0858070-mRNA-1 |
| chr5_4 | CDS | 55099503 | 55099900 | 55099503 | ID=Msa0858070-mRNA-1:cds;Parent=Msa0858070-mRNA-1 |
| chr5_4 | CDS | 55100612 | 55101029 | 55100612 | ID=Msa0858070-mRNA-1:cds;Parent=Msa0858070-mRNA-1 |
| chr5_4 | three_prime_UTR | 55101030 | 55101348 | 55101030 | ID=Msa0858070-mRNA-1:three_prime_utr;Parent=Msa0858070-mRNA-1 |
| Gene Sequence |
| Protein sequence |
