Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0817630 | XP_003616568.1 | 98.155 | 271 | 5 | 0 | 1 | 271 | 1 | 271 | 0.0 | 560 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0817630 | sp|Q8S9H7|DIV_ANTMA | 58.879 | 214 | 76 | 5 | 3 | 206 | 1 | 212 | 2.06e-79 | 245 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0817630 | G7KBX0 | 98.155 | 271 | 5 | 0 | 1 | 271 | 1 | 271 | 0.0 | 560 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0817630 | TF | MYB |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0738190 | Msa0817630 | 0.925827 | 1.081900e-90 | -8.615850e-47 |
| Msa0817630 | Msa0858070 | 0.898664 | 4.250460e-77 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0817630 | MtrunA17_Chr5g0436501 | 98.155 | 271 | 5 | 0 | 1 | 271 | 1 | 271 | 0.0 | 560 |
| Msa0817630 | MtrunA17_Chr7g0241761 | 61.682 | 214 | 73 | 2 | 3 | 209 | 11 | 222 | 2.44e-89 | 267 |
| Msa0817630 | MtrunA17_Chr5g0429521 | 61.386 | 202 | 71 | 3 | 2 | 198 | 4 | 203 | 1.05e-84 | 255 |
| Msa0817630 | MtrunA17_Chr3g0104071 | 64.796 | 196 | 56 | 4 | 16 | 200 | 27 | 220 | 1.76e-83 | 253 |
| Msa0817630 | MtrunA17_Chr1g0162081 | 52.356 | 191 | 75 | 4 | 25 | 202 | 10 | 197 | 4.55e-57 | 184 |
| Msa0817630 | MtrunA17_Chr1g0210471 | 49.733 | 187 | 80 | 4 | 25 | 204 | 22 | 201 | 2.72e-55 | 178 |
| Msa0817630 | MtrunA17_Chr4g0062741 | 42.857 | 210 | 80 | 5 | 27 | 201 | 8 | 212 | 4.69e-48 | 161 |
| Msa0817630 | MtrunA17_Chr8g0365011 | 43.229 | 192 | 79 | 4 | 25 | 202 | 6 | 181 | 5.14e-39 | 137 |
| Msa0817630 | MtrunA17_Chr2g0331031 | 61.111 | 90 | 33 | 1 | 120 | 209 | 104 | 191 | 1.35e-29 | 114 |
| Msa0817630 | MtrunA17_Chr6g0488471 | 69.863 | 73 | 20 | 1 | 119 | 191 | 91 | 161 | 6.17e-29 | 111 |
| Msa0817630 | MtrunA17_Chr4g0055931 | 64.198 | 81 | 27 | 1 | 120 | 200 | 90 | 168 | 5.81e-28 | 109 |
| Msa0817630 | MtrunA17_Chr8g0389241 | 60.714 | 84 | 30 | 1 | 116 | 199 | 96 | 176 | 5.13e-27 | 105 |
| Msa0817630 | MtrunA17_Chr5g0415191 | 60.000 | 90 | 34 | 1 | 112 | 201 | 89 | 176 | 6.32e-26 | 103 |
| Msa0817630 | MtrunA17_Chr4g0059701 | 71.186 | 59 | 17 | 0 | 113 | 171 | 72 | 130 | 9.57e-25 | 100 |
| Msa0817630 | MtrunA17_Chr5g0409841 | 49.524 | 105 | 49 | 1 | 92 | 192 | 55 | 159 | 7.37e-24 | 96.7 |
| Msa0817630 | MtrunA17_Chr8g0364741 | 40.984 | 122 | 66 | 2 | 25 | 142 | 8 | 127 | 1.40e-23 | 92.8 |
| Msa0817630 | MtrunA17_Chr5g0440741 | 58.025 | 81 | 32 | 2 | 113 | 192 | 89 | 168 | 3.41e-23 | 95.9 |
| Msa0817630 | MtrunA17_Chr5g0440731 | 58.904 | 73 | 29 | 1 | 121 | 192 | 101 | 173 | 6.37e-23 | 95.5 |
| Msa0817630 | MtrunA17_Chr3g0135671 | 51.429 | 105 | 44 | 4 | 90 | 191 | 58 | 158 | 7.52e-23 | 94.0 |
| Msa0817630 | MtrunA17_Chr1g0155181 | 55.882 | 68 | 30 | 0 | 22 | 89 | 2 | 69 | 5.59e-21 | 84.7 |
| Msa0817630 | MtrunA17_Chr3g0092621 | 57.971 | 69 | 27 | 1 | 125 | 193 | 84 | 150 | 1.75e-20 | 85.9 |
| Msa0817630 | MtrunA17_Chr3g0141601 | 53.846 | 65 | 30 | 0 | 25 | 89 | 5 | 69 | 2.10e-19 | 80.9 |
| Msa0817630 | MtrunA17_Chr6g0449111 | 52.941 | 68 | 32 | 0 | 22 | 89 | 5 | 72 | 8.71e-19 | 78.6 |
| Msa0817630 | MtrunA17_Chr5g0409831 | 58.904 | 73 | 29 | 1 | 121 | 192 | 73 | 145 | 9.26e-19 | 82.8 |
| Msa0817630 | MtrunA17_Chr5g0432921 | 33.000 | 200 | 119 | 7 | 26 | 218 | 20 | 211 | 1.12e-18 | 82.4 |
| Msa0817630 | MtrunA17_Chr3g0113121 | 40.764 | 157 | 79 | 7 | 48 | 196 | 21 | 171 | 2.53e-18 | 81.3 |
| Msa0817630 | MtrunA17_Chr8g0364731 | 60.294 | 68 | 27 | 0 | 135 | 202 | 11 | 78 | 5.62e-18 | 79.7 |
| Msa0817630 | MtrunA17_Chr7g0255841 | 51.471 | 68 | 33 | 0 | 30 | 97 | 15 | 82 | 9.11e-18 | 76.3 |
| Msa0817630 | MtrunA17_Chr6g0452791 | 57.627 | 59 | 25 | 0 | 125 | 183 | 80 | 138 | 9.37e-18 | 78.6 |
| Msa0817630 | MtrunA17_Chr3g0144631 | 52.381 | 63 | 30 | 0 | 27 | 89 | 16 | 78 | 1.58e-17 | 75.5 |
| Msa0817630 | MtrunA17_Chr5g0404101 | 44.000 | 75 | 42 | 0 | 15 | 89 | 7 | 81 | 4.05e-17 | 74.7 |
| Msa0817630 | MtrunA17_Chr5g0432941 | 30.000 | 200 | 124 | 5 | 26 | 218 | 21 | 211 | 6.15e-17 | 77.8 |
| Msa0817630 | MtrunA17_Chr3g0081631 | 47.945 | 73 | 37 | 1 | 18 | 89 | 1 | 73 | 1.29e-16 | 73.2 |
| Msa0817630 | MtrunA17_Chr8g0373111 | 49.231 | 65 | 32 | 1 | 25 | 89 | 3 | 66 | 1.95e-15 | 69.7 |
| Msa0817630 | MtrunA17_Chr8g0373121 | 49.231 | 65 | 32 | 1 | 25 | 89 | 3 | 66 | 1.95e-15 | 69.7 |
| Msa0817630 | MtrunA17_Chr8g0373131 | 49.231 | 65 | 32 | 1 | 25 | 89 | 3 | 66 | 1.96e-15 | 69.7 |
| Msa0817630 | MtrunA17_Chr8g0373101 | 50.820 | 61 | 29 | 1 | 27 | 87 | 5 | 64 | 6.70e-14 | 65.5 |
| Msa0817630 | MtrunA17_Chr1g0192061 | 55.714 | 70 | 29 | 2 | 127 | 195 | 63 | 131 | 2.35e-13 | 67.0 |
| Msa0817630 | MtrunA17_Chr5g0404301 | 50.820 | 61 | 29 | 1 | 27 | 87 | 4 | 63 | 3.08e-13 | 63.2 |
| Msa0817630 | MtrunA17_Chr3g0141641 | 38.806 | 67 | 41 | 0 | 27 | 93 | 3 | 69 | 3.13e-13 | 64.3 |
| Msa0817630 | MtrunA17_Chr8g0370121 | 50.000 | 50 | 25 | 0 | 122 | 171 | 97 | 146 | 8.39e-12 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0817630 | AT2G38090.1 | 59.242 | 211 | 70 | 2 | 3 | 199 | 5 | 213 | 1.61e-85 | 258 |
| Msa0817630 | AT3G11280.2 | 59.459 | 222 | 71 | 6 | 3 | 212 | 1 | 215 | 3.32e-80 | 243 |
| Msa0817630 | AT3G11280.1 | 59.459 | 222 | 71 | 6 | 3 | 212 | 1 | 215 | 3.32e-80 | 243 |
| Msa0817630 | AT5G05790.2 | 56.828 | 227 | 88 | 6 | 3 | 222 | 4 | 227 | 1.61e-79 | 241 |
| Msa0817630 | AT5G05790.1 | 56.828 | 227 | 88 | 6 | 3 | 222 | 4 | 227 | 1.61e-79 | 241 |
| Msa0817630 | AT5G58900.1 | 58.019 | 212 | 70 | 4 | 16 | 210 | 15 | 224 | 6.64e-77 | 235 |
| Msa0817630 | AT5G01200.1 | 53.589 | 209 | 74 | 6 | 6 | 196 | 12 | 215 | 1.15e-66 | 208 |
| Msa0817630 | AT5G08520.1 | 51.087 | 184 | 80 | 2 | 27 | 202 | 12 | 193 | 5.99e-54 | 177 |
| Msa0817630 | AT1G49010.1 | 44.554 | 202 | 82 | 4 | 21 | 194 | 2 | 201 | 6.31e-49 | 164 |
| Msa0817630 | AT5G04760.1 | 43.333 | 210 | 89 | 7 | 25 | 231 | 4 | 186 | 2.13e-46 | 155 |
| Msa0817630 | AT5G23650.1 | 42.654 | 211 | 89 | 5 | 20 | 215 | 6 | 199 | 2.79e-44 | 153 |
| Msa0817630 | AT3G10580.2 | 38.164 | 207 | 98 | 7 | 27 | 231 | 8 | 186 | 2.04e-35 | 127 |
| Msa0817630 | AT3G10580.1 | 38.164 | 207 | 98 | 7 | 27 | 231 | 8 | 186 | 5.11e-35 | 127 |
| Msa0817630 | AT4G09450.1 | 42.515 | 167 | 80 | 4 | 26 | 192 | 6 | 156 | 4.15e-32 | 117 |
| Msa0817630 | AT5G56840.1 | 63.415 | 82 | 29 | 1 | 120 | 201 | 86 | 166 | 1.05e-27 | 107 |
| Msa0817630 | AT5G47390.1 | 66.250 | 80 | 25 | 1 | 112 | 191 | 83 | 160 | 1.37e-27 | 109 |
| Msa0817630 | AT5G61620.1 | 52.941 | 102 | 46 | 1 | 91 | 192 | 74 | 173 | 6.86e-27 | 106 |
| Msa0817630 | AT1G19000.2 | 51.327 | 113 | 48 | 3 | 112 | 219 | 89 | 199 | 3.41e-25 | 101 |
| Msa0817630 | AT1G19000.1 | 51.327 | 113 | 48 | 3 | 112 | 219 | 89 | 199 | 3.41e-25 | 101 |
| Msa0817630 | AT1G70000.1 | 72.131 | 61 | 17 | 0 | 112 | 172 | 85 | 145 | 5.27e-25 | 100 |
| Msa0817630 | AT1G70000.2 | 72.131 | 61 | 17 | 0 | 112 | 172 | 85 | 145 | 5.27e-25 | 100 |
| Msa0817630 | AT3G16350.1 | 55.238 | 105 | 42 | 2 | 116 | 217 | 125 | 227 | 6.71e-25 | 102 |
| Msa0817630 | AT3G10590.1 | 40.196 | 204 | 96 | 12 | 27 | 216 | 7 | 198 | 5.65e-23 | 94.0 |
| Msa0817630 | AT1G74840.2 | 75.000 | 52 | 13 | 0 | 120 | 171 | 91 | 142 | 5.40e-22 | 92.0 |
| Msa0817630 | AT1G74840.1 | 75.000 | 52 | 13 | 0 | 120 | 171 | 91 | 142 | 7.11e-22 | 92.4 |
| Msa0817630 | AT1G75250.2 | 55.556 | 63 | 28 | 0 | 27 | 89 | 12 | 74 | 8.02e-18 | 77.0 |
| Msa0817630 | AT1G75250.1 | 55.556 | 63 | 28 | 0 | 27 | 89 | 12 | 74 | 9.55e-18 | 77.8 |
| Msa0817630 | AT4G39250.1 | 48.485 | 66 | 34 | 0 | 27 | 92 | 14 | 79 | 2.67e-17 | 75.9 |
| Msa0817630 | AT2G21650.1 | 47.761 | 67 | 35 | 0 | 27 | 93 | 14 | 80 | 5.45e-16 | 72.4 |
| Msa0817630 | AT2G18328.1 | 54.098 | 61 | 27 | 1 | 29 | 89 | 14 | 73 | 5.80e-14 | 66.2 |
| Msa0817630 | AT1G19510.1 | 51.724 | 58 | 28 | 0 | 32 | 89 | 17 | 74 | 6.74e-13 | 63.9 |
| Msa0817630 | AT3G10595.1 | 29.054 | 148 | 81 | 5 | 24 | 171 | 3 | 126 | 2.07e-12 | 64.7 |
Find 83 sgRNAs with CRISPR-Local
Find 127 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATTATTGCAGTTTGCTTTA+AGG | 0.090941 | 5_3:-52966295 | None:intergenic |
| CTCAATCCTTTAACAGAATT+TGG | 0.092721 | 5_3:+52966319 | Msa0817630:three_prime_UTR |
| AATAAGTTCTTATCTAAATT+TGG | 0.139369 | 5_3:+52966541 | Msa0817630:three_prime_UTR |
| TCCACTCCAAATTCTGTTAA+AGG | 0.150880 | 5_3:-52966325 | None:intergenic |
| TAGTAGGTATTTGTGATTTA+AGG | 0.177207 | 5_3:+52964709 | Msa0817630:intron |
| GTCTCCTATGCAGCAGAAAA+TGG | 0.222210 | 5_3:+52966073 | Msa0817630:CDS |
| TATGCTAAGCTGAAAGTTTC+GGG | 0.251091 | 5_3:+52966170 | Msa0817630:CDS |
| CTGATGGGACTTCTAAAGTA+TGG | 0.266546 | 5_3:+52965843 | Msa0817630:CDS |
| ACCAGGAATCGGAACATTTC+CGG | 0.268577 | 5_3:-52964974 | None:intergenic |
| TGCAGTTTGCTTTAAGGTTC+AGG | 0.269442 | 5_3:-52966289 | None:intergenic |
| AGAAGATATGAAAATATTAT+AGG | 0.300464 | 5_3:-52964351 | None:intergenic |
| GCCGGAAATGTTCCGATTCC+TGG | 0.317971 | 5_3:+52964973 | Msa0817630:CDS |
| TTATGCTAAGCTGAAAGTTT+CGG | 0.326332 | 5_3:+52966169 | Msa0817630:CDS |
| CTTCTCTACTCCATTCTGTT+TGG | 0.332735 | 5_3:-52964801 | None:intergenic |
| TAGTGATCATGTCTATCTTT+TGG | 0.334038 | 5_3:+52966396 | Msa0817630:three_prime_UTR |
| AGGAGACACATTGACTAATA+AGG | 0.335368 | 5_3:-52966057 | None:intergenic |
| ACATAAAATAAGGAGAATAA+AGG | 0.337256 | 5_3:-52964747 | None:intergenic |
| TGTGATTTAAGGTATGGATT+TGG | 0.345223 | 5_3:+52964720 | Msa0817630:exon |
| AGGGTGGTGTATAATATTCT+TGG | 0.349156 | 5_3:+52964431 | Msa0817630:five_prime_UTR |
| AGATTCTGTATGATACTAAA+AGG | 0.365142 | 5_3:+52964539 | Msa0817630:five_prime_UTR |
| AAAGTAGAAGTGAGTTCAAA+AGG | 0.365448 | 5_3:-52964120 | None:intergenic |
| TGATGTGATTCAGCAGTATA+GGG | 0.366604 | 5_3:+52964921 | Msa0817630:CDS |
| AGAAGGCATGTGACTGTTAG+AGG | 0.384719 | 5_3:+52965057 | Msa0817630:CDS |
| AAGATTGCTGAGATGATTCC+TGG | 0.391655 | 5_3:+52964886 | Msa0817630:CDS |
| ATATTCAACCTTTCTTTAAG+AGG | 0.395823 | 5_3:+52964411 | Msa0817630:five_prime_UTR |
| GGTATTTGTGATTTAAGGTA+TGG | 0.403756 | 5_3:+52964714 | Msa0817630:intron |
| TTTGTATCCAACATAAAATA+AGG | 0.405866 | 5_3:-52964757 | None:intergenic |
| TTGATGTGATTCAGCAGTAT+AGG | 0.412480 | 5_3:+52964920 | Msa0817630:CDS |
| ATAAGGCAAAAGGTTTCTTC+TGG | 0.415949 | 5_3:+52965951 | Msa0817630:CDS |
| GCAATCTTAAGCCATCTATC+TGG | 0.417904 | 5_3:-52964871 | None:intergenic |
| TAAAGTATGGCAAAGGCGAC+TGG | 0.439380 | 5_3:+52965856 | Msa0817630:CDS |
| CATAAAATAAGGAGAATAAA+GGG | 0.445828 | 5_3:-52964746 | None:intergenic |
| GATTCAGCAGTATAGGGAAT+TGG | 0.452633 | 5_3:+52964927 | Msa0817630:CDS |
| TGCTCTTGCTATATATGATA+AGG | 0.457525 | 5_3:+52964843 | Msa0817630:CDS |
| TGAGACACAGCATGTCTGAA+TGG | 0.469648 | 5_3:-52966445 | None:intergenic |
| TACACCACCCTCTTAAAGAA+AGG | 0.470360 | 5_3:-52964419 | None:intergenic |
| ATCATACTTAAAAGATAAAG+AGG | 0.470899 | 5_3:-52966374 | None:intergenic |
| CACTTTGTCGTTTCTTCATG+AGG | 0.474646 | 5_3:-52964215 | None:intergenic |
| ATAAGGATACACCAGATAGA+TGG | 0.475384 | 5_3:+52964860 | Msa0817630:CDS |
| ATGCACAAAAGTACTACATA+AGG | 0.480834 | 5_3:+52965934 | Msa0817630:CDS |
| AGAGGAAGAAAGGTGTTCCT+TGG | 0.489408 | 5_3:+52965091 | Msa0817630:CDS |
| GAGACACAGCATGTCTGAAT+GGG | 0.499137 | 5_3:-52966444 | None:intergenic |
| AAGTGAGTTCAAAAGGAAGA+AGG | 0.507219 | 5_3:-52964113 | None:intergenic |
| AAAGTACTACATAAGGCAAA+AGG | 0.510073 | 5_3:+52965941 | Msa0817630:CDS |
| TATTCAACCTTTCTTTAAGA+GGG | 0.510102 | 5_3:+52964412 | Msa0817630:five_prime_UTR |
| GAAGCAACAATAAAAGCAAA+TGG | 0.511111 | 5_3:-52964193 | None:intergenic |
| GCATGACTTGCCACTTGTGT+TGG | 0.511936 | 5_3:-52965915 | None:intergenic |
| AATAAGGACTTGTTATCTGA+TGG | 0.512003 | 5_3:-52966041 | None:intergenic |
| GTTGATGACATGAAGCTACT+TGG | 0.515565 | 5_3:+52966255 | Msa0817630:three_prime_UTR |
| GAGGCAAGATAACCAGGAAT+CGG | 0.520347 | 5_3:-52964985 | None:intergenic |
| TGATGGATCACCACAAGGGC+AGG | 0.524353 | 5_3:+52966118 | Msa0817630:CDS |
| TCAGCTTAGCATAAGCCTCA+TGG | 0.535139 | 5_3:-52966160 | None:intergenic |
| TCTCCTATGCAGCAGAAAAT+GGG | 0.535185 | 5_3:+52966074 | Msa0817630:CDS |
| AGTACTAGCCACTACAATGA+TGG | 0.540673 | 5_3:+52966101 | Msa0817630:CDS |
| TCTGATCATGAGAGGAAGAA+AGG | 0.544690 | 5_3:+52965081 | Msa0817630:CDS |
| CAAGAGCAAGAGGGTGAGAG+AGG | 0.550777 | 5_3:-52964322 | None:intergenic |
| TCAACCTTTCTTTAAGAGGG+TGG | 0.551667 | 5_3:+52964415 | Msa0817630:five_prime_UTR |
| AAAGAAGAGGCAAGATAACC+AGG | 0.552310 | 5_3:-52964991 | None:intergenic |
| AAGAGAGTTACCAAACAGAA+TGG | 0.556624 | 5_3:+52964791 | Msa0817630:CDS |
| TCCTTTAACAGAATTTGGAG+TGG | 0.565291 | 5_3:+52966324 | Msa0817630:three_prime_UTR |
| GACAAAGACTCCAACACAAG+TGG | 0.578965 | 5_3:+52965905 | Msa0817630:CDS |
| AGGCAAAAGGTTTCTTCTGG+AGG | 0.582472 | 5_3:+52965954 | Msa0817630:CDS |
| ACTATGATGGAAACAGAAGA+AGG | 0.582942 | 5_3:+52965040 | Msa0817630:CDS |
| TCATAGAGATCCTGCCCTTG+TGG | 0.585420 | 5_3:-52966128 | None:intergenic |
| CTTGGACTGAAGAGGAACAT+AGG | 0.585629 | 5_3:+52965109 | Msa0817630:CDS |
| CTATGTTCCTCTTCAGTCCA+AGG | 0.586053 | 5_3:-52965108 | None:intergenic |
| CTATGCAGCAGAAAATGGGT+TGG | 0.587490 | 5_3:+52966078 | Msa0817630:CDS |
| AGGTGTTCCTTGGACTGAAG+AGG | 0.592742 | 5_3:+52965101 | Msa0817630:CDS |
| AAGAGCAAGAGGGTGAGAGA+GGG | 0.594367 | 5_3:-52964321 | None:intergenic |
| TAGGAACATTCAAGAGCAAG+AGG | 0.598552 | 5_3:-52964332 | None:intergenic |
| GTTGTGATATCATGAATGCT+TGG | 0.600782 | 5_3:-52965993 | None:intergenic |
| CTATGACTGCTCTTTCCATG+AGG | 0.601527 | 5_3:+52966145 | Msa0817630:CDS |
| GCTTCTAGAGACTTCAACAA+AGG | 0.612158 | 5_3:+52966203 | Msa0817630:CDS |
| GATGTGAGTGAAATTGAGGC+CGG | 0.613302 | 5_3:+52964955 | Msa0817630:CDS |
| TACAATGATGGATCACCACA+AGG | 0.622159 | 5_3:+52966113 | Msa0817630:CDS |
| AACTTCAAAGGTGAAAGAAG+AGG | 0.626616 | 5_3:-52965004 | None:intergenic |
| ACATCAATAACAGTCTTACC+AGG | 0.630015 | 5_3:-52964904 | None:intergenic |
| TTAGAGGTTCTGATCATGAG+AGG | 0.633143 | 5_3:+52965073 | Msa0817630:CDS |
| GTGGTGATCCATCATTGTAG+TGG | 0.633599 | 5_3:-52966109 | None:intergenic |
| AGAAGATGTGAGTGAAATTG+AGG | 0.635385 | 5_3:+52964951 | Msa0817630:CDS |
| GGACTTCTAAAGTATGGCAA+AGG | 0.636983 | 5_3:+52965849 | Msa0817630:CDS |
| AGGAACATTCAAGAGCAAGA+GGG | 0.687281 | 5_3:-52964331 | None:intergenic |
| ACAATGATGGATCACCACAA+GGG | 0.720384 | 5_3:+52966114 | Msa0817630:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGAAGATATGAAAATATTAT+AGG | - | chr5_3:52964354-52964373 | None:intergenic | 15.0% |
| !! | AAATAAAAAACCTAAAATCT+AGG | - | chr5_3:52965472-52965491 | None:intergenic | 15.0% |
| !!! | ATAACAAGTAACTTTTAAAA+GGG | - | chr5_3:52965646-52965665 | None:intergenic | 15.0% |
| !!! | AATAACAAGTAACTTTTAAA+AGG | - | chr5_3:52965647-52965666 | None:intergenic | 15.0% |
| !!! | AATAACAAGTAACTTTTAAA+CGG | - | chr5_3:52965647-52965666 | None:intergenic | 15.0% |
| !! | AATAAGTTCTTATCTAAATT+TGG | + | chr5_3:52966541-52966560 | Msa0817630:three_prime_UTR | 15.0% |
| !!! | ATCTATCTTTTTTTCTCTAT+TGG | + | chr5_3:52964281-52964300 | Msa0817630:five_prime_UTR | 20.0% |
| !!! | TTCATATCTTCTATCTTTTT+TGG | + | chr5_3:52964362-52964381 | Msa0817630:five_prime_UTR | 20.0% |
| !! | CATAAAATAAGGAGAATAAA+GGG | - | chr5_3:52964749-52964768 | None:intergenic | 20.0% |
| !! | ACATAAAATAAGGAGAATAA+AGG | - | chr5_3:52964750-52964769 | None:intergenic | 20.0% |
| !!! | TTATTCTCCTTATTTTATGT+TGG | + | chr5_3:52964750-52964769 | Msa0817630:CDS | 20.0% |
| !! | TTTGTATCCAACATAAAATA+AGG | - | chr5_3:52964760-52964779 | None:intergenic | 20.0% |
| !!! | ATTTTATGTTGGATACAAAT+TGG | + | chr5_3:52964761-52964780 | Msa0817630:CDS | 20.0% |
| !!! | ATTTCATTTTCCTAGATTTT+AGG | + | chr5_3:52965459-52965478 | Msa0817630:intron | 20.0% |
| !!! | TAACAAGTAACTTTTAAAAG+GGG | - | chr5_3:52965645-52965664 | None:intergenic | 20.0% |
| !! | ATTGAACTTGATTATTAGTT+TGG | + | chr5_3:52965664-52965683 | Msa0817630:intron | 20.0% |
| !!! | ATAACAAGTAACTTTTAAAC+GGG | - | chr5_3:52965775-52965794 | None:intergenic | 20.0% |
| !! | ATCATACTTAAAAGATAAAG+AGG | - | chr5_3:52966377-52966396 | None:intergenic | 20.0% |
| ! | ATCAAACAAAGGAAAAAAGA+TGG | - | chr5_3:52964253-52964272 | None:intergenic | 25.0% |
| !! | GATTCTTTTCAATCAAACAA+AGG | - | chr5_3:52964264-52964283 | None:intergenic | 25.0% |
| ! | ATATTCAACCTTTCTTTAAG+AGG | + | chr5_3:52964411-52964430 | Msa0817630:five_prime_UTR | 25.0% |
| !! | TATTCAACCTTTCTTTAAGA+GGG | + | chr5_3:52964412-52964431 | Msa0817630:five_prime_UTR | 25.0% |
| !!! | TTTGAGACTATTGATTTTGA+TGG | - | chr5_3:52964463-52964482 | None:intergenic | 25.0% |
| ! | AGATTCTGTATGATACTAAA+AGG | + | chr5_3:52964539-52964558 | Msa0817630:five_prime_UTR | 25.0% |
| !!! | CTATAATTTCCTCTTTTTTG+TGG | + | chr5_3:52964654-52964673 | Msa0817630:intron | 25.0% |
| ! | CATAAAAAACCACAAAAAAG+AGG | - | chr5_3:52964666-52964685 | None:intergenic | 25.0% |
| ! | TAGTAGGTATTTGTGATTTA+AGG | + | chr5_3:52964709-52964728 | Msa0817630:intron | 25.0% |
| ! | ATAAGACAATTGAAGATTCT+AGG | - | chr5_3:52965148-52965167 | None:intergenic | 25.0% |
| !!! | TTGTTTTACTCTTTTCTCTA+TGG | + | chr5_3:52965176-52965195 | Msa0817630:intron | 25.0% |
| ! | CTAGAAAAATTGAAGTAAGA+CGG | + | chr5_3:52965239-52965258 | Msa0817630:intron | 25.0% |
| ! | AGAGACGGAAAAAATAAAAT+CGG | + | chr5_3:52965267-52965286 | Msa0817630:intron | 25.0% |
| ! | TAGCTAATGAATCATATTCA+TGG | + | chr5_3:52965408-52965427 | Msa0817630:intron | 25.0% |
| !!! | ATGTTAAATTGAGTTGGTTT+AGG | + | chr5_3:52965736-52965755 | Msa0817630:intron | 25.0% |
| !! | TAACAAGTAACTTTTAAACG+GGG | - | chr5_3:52965774-52965793 | None:intergenic | 25.0% |
| AAAGTAGAAGTGAGTTCAAA+AGG | - | chr5_3:52964123-52964142 | None:intergenic | 30.0% | |
| GAAGCAACAATAAAAGCAAA+TGG | - | chr5_3:52964196-52964215 | None:intergenic | 30.0% | |
| ! | AAAACCAATCCTTTTGTAGT+AGG | + | chr5_3:52964693-52964712 | Msa0817630:intron | 30.0% |
| AATACCTACTACAAAAGGAT+TGG | - | chr5_3:52964700-52964719 | None:intergenic | 30.0% | |
| TCACAAATACCTACTACAAA+AGG | - | chr5_3:52964705-52964724 | None:intergenic | 30.0% | |
| ! | GGTATTTGTGATTTAAGGTA+TGG | + | chr5_3:52964714-52964733 | Msa0817630:intron | 30.0% |
| ! | TGTGATTTAAGGTATGGATT+TGG | + | chr5_3:52964720-52964739 | Msa0817630:exon | 30.0% |
| ! | TGCTCTTGCTATATATGATA+AGG | + | chr5_3:52964843-52964862 | Msa0817630:CDS | 30.0% |
| !! | TTGTTTTTCGACAACTTCAA+AGG | - | chr5_3:52965019-52965038 | None:intergenic | 30.0% |
| GTCGAAAAACAAAACTATGA+TGG | + | chr5_3:52965027-52965046 | Msa0817630:CDS | 30.0% | |
| !!! | GGAAGTTTTTTCTTAGATTG+TGG | + | chr5_3:52965197-52965216 | Msa0817630:intron | 30.0% |
| !!! | AGTTTTTTCTTAGATTGTGG+AGG | + | chr5_3:52965200-52965219 | Msa0817630:intron | 30.0% |
| !! | GTAACTTTTAAAAGGGGTAA+AGG | - | chr5_3:52965639-52965658 | None:intergenic | 30.0% |
| ATCGACATGTTAAATTGAGT+TGG | + | chr5_3:52965730-52965749 | Msa0817630:intron | 30.0% | |
| ATTTACTCTTTGTGCATGAT+TGG | + | chr5_3:52965798-52965817 | Msa0817630:intron | 30.0% | |
| !! | ATGCACAAAAGTACTACATA+AGG | + | chr5_3:52965934-52965953 | Msa0817630:CDS | 30.0% |
| ! | AAAGTACTACATAAGGCAAA+AGG | + | chr5_3:52965941-52965960 | Msa0817630:CDS | 30.0% |
| !! | AATAAGGACTTGTTATCTGA+TGG | - | chr5_3:52966044-52966063 | None:intergenic | 30.0% |
| TTATGCTAAGCTGAAAGTTT+CGG | + | chr5_3:52966169-52966188 | Msa0817630:CDS | 30.0% | |
| ! | CATTATTGCAGTTTGCTTTA+AGG | - | chr5_3:52966298-52966317 | None:intergenic | 30.0% |
| CTCAATCCTTTAACAGAATT+TGG | + | chr5_3:52966319-52966338 | Msa0817630:three_prime_UTR | 30.0% | |
| ! | TAGTGATCATGTCTATCTTT+TGG | + | chr5_3:52966396-52966415 | Msa0817630:three_prime_UTR | 30.0% |
| AAGTGAGTTCAAAAGGAAGA+AGG | - | chr5_3:52964116-52964135 | None:intergenic | 35.0% | |
| AGGGTGGTGTATAATATTCT+TGG | + | chr5_3:52964431-52964450 | Msa0817630:five_prime_UTR | 35.0% | |
| AAGAGAGTTACCAAACAGAA+TGG | + | chr5_3:52964791-52964810 | Msa0817630:CDS | 35.0% | |
| ATAAGGATACACCAGATAGA+TGG | + | chr5_3:52964860-52964879 | Msa0817630:CDS | 35.0% | |
| ACATCAATAACAGTCTTACC+AGG | - | chr5_3:52964907-52964926 | None:intergenic | 35.0% | |
| TTGATGTGATTCAGCAGTAT+AGG | + | chr5_3:52964920-52964939 | Msa0817630:CDS | 35.0% | |
| TGATGTGATTCAGCAGTATA+GGG | + | chr5_3:52964921-52964940 | Msa0817630:CDS | 35.0% | |
| AGAAGATGTGAGTGAAATTG+AGG | + | chr5_3:52964951-52964970 | Msa0817630:CDS | 35.0% | |
| AACTTCAAAGGTGAAAGAAG+AGG | - | chr5_3:52965007-52965026 | None:intergenic | 35.0% | |
| ! | ACTATGATGGAAACAGAAGA+AGG | + | chr5_3:52965040-52965059 | Msa0817630:CDS | 35.0% |
| GAGTCTGTAACACTTAATGA+TGG | + | chr5_3:52965301-52965320 | Msa0817630:intron | 35.0% | |
| ! | GTAACTTTTAAACGGGGTAA+AGG | - | chr5_3:52965768-52965787 | None:intergenic | 35.0% |
| TTTGTGCATGATTGGAATCA+CGG | + | chr5_3:52965806-52965825 | Msa0817630:intron | 35.0% | |
| ATAAGGCAAAAGGTTTCTTC+TGG | + | chr5_3:52965951-52965970 | Msa0817630:CDS | 35.0% | |
| GTTGTGATATCATGAATGCT+TGG | - | chr5_3:52965996-52966015 | None:intergenic | 35.0% | |
| ! | AGGAGACACATTGACTAATA+AGG | - | chr5_3:52966060-52966079 | None:intergenic | 35.0% |
| TATGCTAAGCTGAAAGTTTC+GGG | + | chr5_3:52966170-52966189 | Msa0817630:CDS | 35.0% | |
| !! | TTCAACAAAGGAGCTGTTTT+TGG | + | chr5_3:52966215-52966234 | Msa0817630:CDS | 35.0% |
| TCCTTTAACAGAATTTGGAG+TGG | + | chr5_3:52966324-52966343 | Msa0817630:three_prime_UTR | 35.0% | |
| TCCACTCCAAATTCTGTTAA+AGG | - | chr5_3:52966328-52966347 | None:intergenic | 35.0% | |
| ! | CACTTTGTCGTTTCTTCATG+AGG | - | chr5_3:52964218-52964237 | None:intergenic | 40.0% |
| AGGAACATTCAAGAGCAAGA+GGG | - | chr5_3:52964334-52964353 | None:intergenic | 40.0% | |
| TAGGAACATTCAAGAGCAAG+AGG | - | chr5_3:52964335-52964354 | None:intergenic | 40.0% | |
| ! | TCAACCTTTCTTTAAGAGGG+TGG | + | chr5_3:52964415-52964434 | Msa0817630:five_prime_UTR | 40.0% |
| TACACCACCCTCTTAAAGAA+AGG | - | chr5_3:52964422-52964441 | None:intergenic | 40.0% | |
| CTTCTCTACTCCATTCTGTT+TGG | - | chr5_3:52964804-52964823 | None:intergenic | 40.0% | |
| GCAATCTTAAGCCATCTATC+TGG | - | chr5_3:52964874-52964893 | None:intergenic | 40.0% | |
| AAGATTGCTGAGATGATTCC+TGG | + | chr5_3:52964886-52964905 | Msa0817630:CDS | 40.0% | |
| GATTCAGCAGTATAGGGAAT+TGG | + | chr5_3:52964927-52964946 | Msa0817630:CDS | 40.0% | |
| AAAGAAGAGGCAAGATAACC+AGG | - | chr5_3:52964994-52965013 | None:intergenic | 40.0% | |
| ! | TTAGAGGTTCTGATCATGAG+AGG | + | chr5_3:52965073-52965092 | Msa0817630:CDS | 40.0% |
| TCTGATCATGAGAGGAAGAA+AGG | + | chr5_3:52965081-52965100 | Msa0817630:CDS | 40.0% | |
| AGTAAGACGGAAGTTAGAGA+CGG | + | chr5_3:52965252-52965271 | Msa0817630:intron | 40.0% | |
| GTAAAGGCAAACTCCAATTG+AGG | - | chr5_3:52965623-52965642 | None:intergenic | 40.0% | |
| ! | GCATACAGACGTTTTCTGAT+GGG | + | chr5_3:52965828-52965847 | Msa0817630:intron | 40.0% |
| !! | CTGATGGGACTTCTAAAGTA+TGG | + | chr5_3:52965843-52965862 | Msa0817630:CDS | 40.0% |
| !! | GGACTTCTAAAGTATGGCAA+AGG | + | chr5_3:52965849-52965868 | Msa0817630:CDS | 40.0% |
| TCTCCTATGCAGCAGAAAAT+GGG | + | chr5_3:52966074-52966093 | Msa0817630:CDS | 40.0% | |
| AGTACTAGCCACTACAATGA+TGG | + | chr5_3:52966101-52966120 | Msa0817630:CDS | 40.0% | |
| !! | TACAATGATGGATCACCACA+AGG | + | chr5_3:52966113-52966132 | Msa0817630:CDS | 40.0% |
| !! | ACAATGATGGATCACCACAA+GGG | + | chr5_3:52966114-52966133 | Msa0817630:CDS | 40.0% |
| ! | GCTTCTAGAGACTTCAACAA+AGG | + | chr5_3:52966203-52966222 | Msa0817630:CDS | 40.0% |
| !! | GTTGATGACATGAAGCTACT+TGG | + | chr5_3:52966255-52966274 | Msa0817630:three_prime_UTR | 40.0% |
| !! | TGCAGTTTGCTTTAAGGTTC+AGG | - | chr5_3:52966292-52966311 | None:intergenic | 40.0% |
| GATGTGAGTGAAATTGAGGC+CGG | + | chr5_3:52964955-52964974 | Msa0817630:CDS | 45.0% | |
| ACCAGGAATCGGAACATTTC+CGG | - | chr5_3:52964977-52964996 | None:intergenic | 45.0% | |
| GAGGCAAGATAACCAGGAAT+CGG | - | chr5_3:52964988-52965007 | None:intergenic | 45.0% | |
| ! | AGAAGGCATGTGACTGTTAG+AGG | + | chr5_3:52965057-52965076 | Msa0817630:CDS | 45.0% |
| !! | AGAGGAAGAAAGGTGTTCCT+TGG | + | chr5_3:52965091-52965110 | Msa0817630:CDS | 45.0% |
| CTATGTTCCTCTTCAGTCCA+AGG | - | chr5_3:52965111-52965130 | None:intergenic | 45.0% | |
| CTTGGACTGAAGAGGAACAT+AGG | + | chr5_3:52965109-52965128 | Msa0817630:CDS | 45.0% | |
| GCAGATAATGCAGCCTCAAT+TGG | + | chr5_3:52965607-52965626 | Msa0817630:intron | 45.0% | |
| ! | GGCATACAGACGTTTTCTGA+TGG | + | chr5_3:52965827-52965846 | Msa0817630:intron | 45.0% |
| TAAAGTATGGCAAAGGCGAC+TGG | + | chr5_3:52965856-52965875 | Msa0817630:CDS | 45.0% | |
| GACAAAGACTCCAACACAAG+TGG | + | chr5_3:52965905-52965924 | Msa0817630:CDS | 45.0% | |
| AGGCAAAAGGTTTCTTCTGG+AGG | + | chr5_3:52965954-52965973 | Msa0817630:CDS | 45.0% | |
| GTCTCCTATGCAGCAGAAAA+TGG | + | chr5_3:52966073-52966092 | Msa0817630:CDS | 45.0% | |
| ! | CAACCCATTTTCTGCTGCAT+AGG | - | chr5_3:52966080-52966099 | None:intergenic | 45.0% |
| CTATGCAGCAGAAAATGGGT+TGG | + | chr5_3:52966078-52966097 | Msa0817630:CDS | 45.0% | |
| GTGGTGATCCATCATTGTAG+TGG | - | chr5_3:52966112-52966131 | None:intergenic | 45.0% | |
| CTATGACTGCTCTTTCCATG+AGG | + | chr5_3:52966145-52966164 | Msa0817630:CDS | 45.0% | |
| TCAGCTTAGCATAAGCCTCA+TGG | - | chr5_3:52966163-52966182 | None:intergenic | 45.0% | |
| GAGACACAGCATGTCTGAAT+GGG | - | chr5_3:52966447-52966466 | None:intergenic | 45.0% | |
| TGAGACACAGCATGTCTGAA+TGG | - | chr5_3:52966448-52966467 | None:intergenic | 45.0% | |
| AAGAGCAAGAGGGTGAGAGA+GGG | - | chr5_3:52964324-52964343 | None:intergenic | 50.0% | |
| AGGTGTTCCTTGGACTGAAG+AGG | + | chr5_3:52965101-52965120 | Msa0817630:CDS | 50.0% | |
| ! | GCATGACTTGCCACTTGTGT+TGG | - | chr5_3:52965918-52965937 | None:intergenic | 50.0% |
| TCATAGAGATCCTGCCCTTG+TGG | - | chr5_3:52966131-52966150 | None:intergenic | 50.0% | |
| CAAGAGCAAGAGGGTGAGAG+AGG | - | chr5_3:52964325-52964344 | None:intergenic | 55.0% | |
| GCCGGAAATGTTCCGATTCC+TGG | + | chr5_3:52964973-52964992 | Msa0817630:CDS | 55.0% | |
| ! | TGATGGATCACCACAAGGGC+AGG | + | chr5_3:52966118-52966137 | Msa0817630:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_3 | gene | 52964066 | 52966574 | 52964066 | ID=Msa0817630;Name=Msa0817630 |
| chr5_3 | mRNA | 52964066 | 52966574 | 52964066 | ID=Msa0817630-mRNA-1;Parent=Msa0817630;Name=Msa0817630-mRNA-1;_AED=0.05;_eAED=0.05;_QI=513|1|1|1|0.5|0.33|3|321|271 |
| chr5_3 | exon | 52964066 | 52964560 | 52964066 | ID=Msa0817630-mRNA-1:exon:15922;Parent=Msa0817630-mRNA-1 |
| chr5_3 | exon | 52964715 | 52965130 | 52964715 | ID=Msa0817630-mRNA-1:exon:15923;Parent=Msa0817630-mRNA-1 |
| chr5_3 | exon | 52965836 | 52966574 | 52965836 | ID=Msa0817630-mRNA-1:exon:15924;Parent=Msa0817630-mRNA-1 |
| chr5_3 | five_prime_UTR | 52964066 | 52964560 | 52964066 | ID=Msa0817630-mRNA-1:five_prime_utr;Parent=Msa0817630-mRNA-1 |
| chr5_3 | five_prime_UTR | 52964715 | 52964732 | 52964715 | ID=Msa0817630-mRNA-1:five_prime_utr;Parent=Msa0817630-mRNA-1 |
| chr5_3 | CDS | 52964733 | 52965130 | 52964733 | ID=Msa0817630-mRNA-1:cds;Parent=Msa0817630-mRNA-1 |
| chr5_3 | CDS | 52965836 | 52966253 | 52965836 | ID=Msa0817630-mRNA-1:cds;Parent=Msa0817630-mRNA-1 |
| chr5_3 | three_prime_UTR | 52966254 | 52966574 | 52966254 | ID=Msa0817630-mRNA-1:three_prime_utr;Parent=Msa0817630-mRNA-1 |
| Gene Sequence |
| Protein sequence |
