AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0744440

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0744440 MtrunA17_Chr5g0447621 99.646 565 2 0 1 565 1 565 0.0 1163
Msa0744440 MtrunA17_Chr8g0376011 36.761 457 271 7 74 522 325 771 4.08e-87 286
Msa0744440 MtrunA17_Chr4g0019561 37.363 455 268 10 92 544 748 1187 8.57e-86 289
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0744440 AT5G48160.2 70.087 575 149 10 1 565 13 574 0.0 797
Msa0744440 AT5G48160.1 70.087 575 149 10 1 565 13 574 0.0 797
Msa0744440 AT3G07780.3 67.077 568 170 7 1 565 13 566 0.0 740
Msa0744440 AT3G07780.1 67.077 568 170 7 1 565 13 566 0.0 740
Msa0744440 AT3G07780.2 67.077 568 170 7 1 565 24 577 0.0 739
Msa0744440 AT3G63500.1 33.400 500 294 9 13 506 374 840 1.99e-78 265
Msa0744440 AT3G63500.2 36.105 421 251 6 92 506 707 1115 2.12e-77 266
Msa0744440 AT3G63500.3 36.105 421 251 6 92 506 707 1115 2.12e-77 266
Msa0744440 AT1G14740.1 34.396 439 243 6 89 522 305 703 7.12e-73 247

Find 159 sgRNAs with CRISPR-Local

Find 183 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAATTTAAACTTGAAAATT+AGG 0.148834 5_1:-65222843 Msa0744440:three_prime_UTR
TCCCTGAATTCCAGTATTTA+TGG 0.176236 5_1:+65224630 None:intergenic
CTAATGACTGCCACCCTTTC+CGG 0.193878 5_1:-65223103 Msa0744440:CDS
ATTAATTATAAGCACTTAAT+AGG 0.209293 5_1:+65222861 None:intergenic
GTAAAGGAGATCATGGATTT+TGG 0.213920 5_1:+65223154 None:intergenic
AAATATACTTAGCAACTAAT+TGG 0.214209 5_1:+65222749 None:intergenic
TGCCATAAATACTGGAATTC+AGG 0.237888 5_1:-65224632 Msa0744440:CDS
CTTGAGTTCTTCTGCTTCTC+TGG 0.253475 5_1:+65223478 None:intergenic
GGCACTGATCCACTCTTTAC+AGG 0.283245 5_1:+65224328 None:intergenic
CATCAGCTGGAAGCTGGTTT+TGG 0.287912 5_1:+65224527 None:intergenic
TGGTTCAATTCCATTGCATT+TGG 0.288651 5_1:-65222987 Msa0744440:three_prime_UTR
ATCTTTGTCTCTTGTTTCTT+CGG 0.301294 5_1:-65225082 Msa0744440:CDS
TTAGATTGACTGTCTGCTTT+GGG 0.315236 5_1:+65223122 None:intergenic
GGTTCAATTCCATTGCATTT+GGG 0.319689 5_1:-65222986 Msa0744440:three_prime_UTR
ATCCTTGTTGCCATAAATAC+TGG 0.341420 5_1:-65224640 Msa0744440:CDS
CTTCTGTTTCAAATAGTTGC+TGG 0.354580 5_1:+65223286 None:intergenic
TCCCTCCACGACAGCAATCC+CGG 0.370544 5_1:-65225122 Msa0744440:CDS
ATCGTGGGATGAACCTCTTT+TGG 0.374814 5_1:-65224147 Msa0744440:CDS
ATTAGATTGACTGTCTGCTT+TGG 0.379288 5_1:+65223121 None:intergenic
TGGCCTCATTAGCCTTCAAT+TGG 0.386901 5_1:+65223375 None:intergenic
GAGCCTCTCAGCCTCTCGTT+TGG 0.409388 5_1:+65223355 None:intergenic
TGTTCTATGCAGTGTTTGTA+TGG 0.409646 5_1:-65222894 Msa0744440:three_prime_UTR
TAGAAATTACAACCAACAAT+TGG 0.419727 5_1:+65223057 None:intergenic
TGCGGACAGAATCAGTCTTC+AGG 0.422728 5_1:-65224878 Msa0744440:CDS
GACATTGATTCGAGCTCATC+GGG 0.423325 5_1:-65224674 Msa0744440:CDS
GTATGAGAAAATCAAATTGC+AGG 0.425425 5_1:-65223231 Msa0744440:CDS
TTTGTTAGTAGGATCTTTCA+TGG 0.429043 5_1:-65224181 Msa0744440:CDS
GATGTGATTTGTGTTCTCAT+TGG 0.433032 5_1:-65224399 Msa0744440:CDS
CACCTGAAAACCATTTCACT+TGG 0.434640 5_1:+65224307 None:intergenic
GGCTGATATGTTCCAATTGA+AGG 0.436823 5_1:-65223387 Msa0744440:CDS
ATAAGTTGAAATTCGAAACT+AGG 0.440679 5_1:-65222919 Msa0744440:three_prime_UTR
GATAGCATCAGCAGTAGGCC+AGG 0.451734 5_1:+65224975 None:intergenic
CACTTTCAGTAGGTGATTCA+CGG 0.453441 5_1:+65225013 None:intergenic
TGGATCAGTGCCAAGTGAAA+TGG 0.456270 5_1:-65224317 Msa0744440:CDS
TCTTGCGAAGTCTGATAAAT+CGG 0.461104 5_1:-65223321 Msa0744440:CDS
CCCGGGATTGCTGTCGTGGA+GGG 0.462782 5_1:+65225121 None:intergenic
GCCATAAATACTGGAATTCA+GGG 0.463071 5_1:-65224631 Msa0744440:CDS
CCAGGCTCTTTGGAGAGTCT+AGG 0.472168 5_1:+65223672 None:intergenic
GATCGTCACACTTCCAAAAG+AGG 0.472222 5_1:+65224134 None:intergenic
ATACTCATCACGGGTTTGTC+CGG 0.472397 5_1:+65223084 None:intergenic
TGGAAGGTTGATTGCTCCAC+AGG 0.475183 5_1:-65223636 Msa0744440:CDS
TGGAAGCTAAAGCAGCATGC+AGG 0.476217 5_1:-65224084 Msa0744440:CDS
ACTCTTTGTCAATTGTATAA+CGG 0.476695 5_1:+65223010 None:intergenic
GTTCAATTCCATTGCATTTG+GGG 0.478354 5_1:-65222985 Msa0744440:three_prime_UTR
TCCAACATTGTGCACCATCA+TGG 0.480513 5_1:-65224234 Msa0744440:CDS
GGCCATTGTAAAGGAGATCA+TGG 0.483195 5_1:+65223147 None:intergenic
ATGAGTATTGGTCCAATTGT+TGG 0.486119 5_1:-65223069 Msa0744440:three_prime_UTR
CCAACCCGGGATTGCTGTCG+TGG 0.486754 5_1:+65225117 None:intergenic
CAGAATTGCTGAAGTGGTGC+AGG 0.488827 5_1:-65223603 Msa0744440:CDS
CGACGAATAACTGACTGATC+AGG 0.490361 5_1:+65224910 None:intergenic
TGCTATCGCTGCGAAGAAGA+TGG 0.495976 5_1:-65224959 Msa0744440:CDS
CTTTGGGAGGGCCATTGTAA+AGG 0.496266 5_1:+65223138 None:intergenic
GATTGACTGTCTGCTTTGGG+AGG 0.497767 5_1:+65223125 None:intergenic
ATTGCAGAAGCCACTGTTGT+TGG 0.498959 5_1:+65224483 None:intergenic
GGACTTCGAGTTATCCTTGA+GGG 0.499090 5_1:-65224775 Msa0744440:CDS
GCTTGTCTCCTGAGGATCCT+AGG 0.499181 5_1:-65225053 Msa0744440:CDS
AACAATCATCAGCTGGAAGC+TGG 0.500810 5_1:+65224521 None:intergenic
GCAGGAGGCTATACGAAAGA+TGG 0.504278 5_1:-65223585 Msa0744440:CDS
GCACTATAAACCTCTGTGAA+TGG 0.506968 5_1:-65222963 Msa0744440:three_prime_UTR
ACTTCGCAAGAGCAATCCTC+TGG 0.508330 5_1:+65223333 None:intergenic
GCAAATAAGTTGCTCCCGTA+TGG 0.512263 5_1:+65224360 None:intergenic
TCAGTCTTCAGGACATTGCT+CGG 0.513007 5_1:-65224867 Msa0744440:CDS
GGACTTCAGAACTGTTAGGC+TGG 0.513412 5_1:-65224270 Msa0744440:CDS
CTCACACAGATTGTGCCATA+CGG 0.513413 5_1:-65224375 Msa0744440:CDS
CCTAGACTCTCCAAAGAGCC+TGG 0.513548 5_1:-65223672 Msa0744440:CDS
CCCTCCACGACAGCAATCCC+GGG 0.515207 5_1:-65225121 Msa0744440:CDS
GTAAATACATGCCGTTGGAT+TGG 0.515809 5_1:-65224421 Msa0744440:CDS
GAAGGCTCGCTTAGCTGTTG+AGG 0.516614 5_1:-65223528 Msa0744440:CDS
ATCACGGGTTTGTCCGGAAA+GGG 0.518375 5_1:+65223090 None:intergenic
GACAAACCCGTGATGAGTAT+TGG 0.519442 5_1:-65223081 Msa0744440:exon
GAGTTTGACCTAGGATCCTC+AGG 0.521068 5_1:+65225045 None:intergenic
GTTCCAATTGAAGGCTAATG+AGG 0.521411 5_1:-65223378 Msa0744440:CDS
TTGAAGTAAATACATGCCGT+TGG 0.522716 5_1:-65224426 Msa0744440:CDS
CCCATGATGGTGCACAATGT+TGG 0.522804 5_1:+65224233 None:intergenic
GACTTCAGAACTGTTAGGCT+GGG 0.527791 5_1:-65224269 Msa0744440:CDS
GGCTGCGACAGACTAATGTT+AGG 0.528186 5_1:+65224598 None:intergenic
ATTGACTGTCTGCTTTGGGA+GGG 0.528716 5_1:+65223126 None:intergenic
AGAAGCAGAAGAACTCAAGA+TGG 0.529192 5_1:-65223474 Msa0744440:CDS
CAGTGTTTGTATGGATATGC+TGG 0.529285 5_1:-65222885 Msa0744440:three_prime_UTR
ACGGGAGCAACTTATTTGCA+TGG 0.531202 5_1:-65224356 Msa0744440:CDS
AGACATTGATTCGAGCTCAT+CGG 0.534133 5_1:-65224675 Msa0744440:CDS
ATGAGCTCGAATCAATGTCT+TGG 0.537389 5_1:+65224678 None:intergenic
GCTGCGAAGAAGATGGAGAA+TGG 0.541377 5_1:-65224952 Msa0744440:CDS
AGCCTGGAAAATGTAGAAAG+TGG 0.542593 5_1:-65223656 Msa0744440:CDS
TAAACCTCTGTGAATGGAAG+AGG 0.543524 5_1:-65222957 Msa0744440:three_prime_UTR
GTTATCCTTGAGGGAGGGAA+TGG 0.544054 5_1:-65224766 Msa0744440:CDS
TTGGAAGTGTGACGATCTCA+AGG 0.544756 5_1:-65224128 Msa0744440:CDS
CCAACATTGTGCACCATCAT+GGG 0.546323 5_1:-65224233 Msa0744440:CDS
CGACAGCAATCCCGGGTTGG+AGG 0.547481 5_1:-65225114 Msa0744440:CDS
TGTTTGCAGTCCTCCAACCC+GGG 0.548493 5_1:+65225104 None:intergenic
CAGTCTTCAGGACATTGCTC+GGG 0.548827 5_1:-65224866 Msa0744440:CDS
ATTCTGTTGCATGCCTCCTG+TGG 0.548889 5_1:+65223620 None:intergenic
ACCCGGGATTGCTGTCGTGG+AGG 0.553588 5_1:+65225120 None:intergenic
TTCATGGACATGTCACCACC+AGG 0.557081 5_1:-65225181 Msa0744440:five_prime_UTR
AAAATCAAGCTCCCTCATCA+AGG 0.557639 5_1:+65224201 None:intergenic
TATTCTACGCAGTGTATGCA+TGG 0.558369 5_1:-65222818 Msa0744440:three_prime_UTR
TCACAAGAACAATCATCAGC+TGG 0.558649 5_1:+65224514 None:intergenic
CGGGAGCAACTTATTTGCAT+GGG 0.558837 5_1:-65224355 Msa0744440:CDS
ACAGAAGTTAAGTGAGGCTG+AGG 0.558933 5_1:-65223270 Msa0744440:CDS
GGAAGCTAAAGCAGCATGCA+GGG 0.560687 5_1:-65224083 Msa0744440:CDS
TCACACAGATTGTGCCATAC+GGG 0.562732 5_1:-65224374 Msa0744440:CDS
TCAGGTGTCAATCATGCAGT+AGG 0.562912 5_1:-65224291 Msa0744440:CDS
ATGTTTGCAGTCCTCCAACC+CGG 0.569198 5_1:+65225103 None:intergenic
GAATACATCAGCATTGAAGA+GGG 0.569622 5_1:+65223176 None:intergenic
ATTGTGCACCATCATGGGAT+GGG 0.570954 5_1:-65224228 Msa0744440:CDS
CTAGAACTAGCACTTTCAGT+AGG 0.578007 5_1:+65225003 None:intergenic
CATTGTGCACCATCATGGGA+TGG 0.578553 5_1:-65224229 Msa0744440:CDS
AGTAGGACTTCAGAACTGTT+AGG 0.579004 5_1:-65224274 Msa0744440:CDS
TTATACAATTGACAAAGAGT+TGG 0.579432 5_1:-65223007 Msa0744440:three_prime_UTR
GGACATTGCTCGGGAAAGAG+TGG 0.584255 5_1:-65224857 Msa0744440:CDS
GGAATACATCAGCATTGAAG+AGG 0.585828 5_1:+65223175 None:intergenic
CAATTGGAACATATCAGCCT+CGG 0.587516 5_1:+65223391 None:intergenic
GCATGCCATTCCCTCCCTCA+AGG 0.588171 5_1:+65224761 None:intergenic
CAGAAGAACTCAAGATGGAG+AGG 0.588684 5_1:-65223469 Msa0744440:CDS
CATCACGGGTTTGTCCGGAA+AGG 0.588989 5_1:+65223089 None:intergenic
TTGTGCACCATCATGGGATG+GGG 0.590598 5_1:-65224227 Msa0744440:CDS
AATTGGACCAATACTCATCA+CGG 0.591741 5_1:+65223074 None:intergenic
AAGGAATCCCCATCCCATGA+TGG 0.593870 5_1:+65224220 None:intergenic
GAAGATGAGAATGTTCAAGA+AGG 0.594556 5_1:-65223546 Msa0744440:CDS
GCTCTAATATAAAACAATTG+TGG 0.597759 5_1:-65225159 Msa0744440:five_prime_UTR
ATGGTAGTAAAGACCATCGT+GGG 0.598320 5_1:-65224162 Msa0744440:CDS
GAGAGGCTGAGAGGCTCCAG+AGG 0.598779 5_1:-65223349 Msa0744440:CDS
ATTGGACCAATACTCATCAC+GGG 0.601698 5_1:+65223075 None:intergenic
CCACGACAGCAATCCCGGGT+TGG 0.602524 5_1:-65225117 Msa0744440:CDS
GGATCCTCGCTTGTCTCCTG+AGG 0.602741 5_1:-65225061 Msa0744440:CDS
TTATAGTGCCCCAAATGCAA+TGG 0.603317 5_1:+65222977 None:intergenic
TTCCAGTATTTATGGCAACA+AGG 0.603589 5_1:+65224638 None:intergenic
AGAAATATCTCAATCAGCGA+TGG 0.603706 5_1:+65224577 None:intergenic
TGGAAAATGTAGAAAGTGGA+AGG 0.603944 5_1:-65223652 Msa0744440:CDS
ATGCAACAGAATTGCTGAAG+TGG 0.604458 5_1:-65223609 Msa0744440:CDS
ATCCATGATCTCCTTTACAA+TGG 0.611502 5_1:-65223149 Msa0744440:CDS
CTTCGAGTTATCCTTGAGGG+AGG 0.614388 5_1:-65224772 Msa0744440:CDS
TTCGAGTTATCCTTGAGGGA+GGG 0.615596 5_1:-65224771 Msa0744440:CDS
GGCTAATGAGGCCAAACGAG+AGG 0.618483 5_1:-65223366 Msa0744440:CDS
AATTGCTGAAGTGGTGCAGG+AGG 0.618880 5_1:-65223600 Msa0744440:CDS
AGGTTATCAGAGTTTGACCT+AGG 0.619181 5_1:+65225036 None:intergenic
TATTCGTCGTGTTTCTAGTG+CGG 0.628975 5_1:-65224896 Msa0744440:CDS
ACGGGTTTGTCCGGAAAGGG+TGG 0.629535 5_1:+65223093 None:intergenic
GTGCTAGTTCTAGAGAAACC+TGG 0.630706 5_1:-65224993 Msa0744440:CDS
TTTGAAACAGAAGTTAAGTG+AGG 0.634933 5_1:-65223276 Msa0744440:CDS
GCAGCGATAGCATCAGCAGT+AGG 0.640719 5_1:+65224970 None:intergenic
TGATCGTGAACTAGCAGACA+AGG 0.644648 5_1:-65223501 Msa0744440:CDS
ACAAATCACATCCAATCCAA+CGG 0.645811 5_1:+65224410 None:intergenic
GGATGGGGATTCCTTGATGA+GGG 0.648889 5_1:-65224212 Msa0744440:CDS
GGGATGGGGATTCCTTGATG+AGG 0.651572 5_1:-65224213 Msa0744440:CDS
AGGCCAAACGAGAGGCTGAG+AGG 0.658904 5_1:-65223358 Msa0744440:CDS
AAGGTTGATTGCTCCACAGG+AGG 0.659574 5_1:-65223633 Msa0744440:CDS
ATGGGTCCTCCTGTAAAGAG+TGG 0.666155 5_1:-65224337 Msa0744440:CDS
TCTCGCTTATCGCAGAGCAG+TGG 0.671798 5_1:-65223203 Msa0744440:CDS
ACTGATCCACTCTTTACAGG+AGG 0.681348 5_1:+65224331 None:intergenic
CATGGTAGTAAAGACCATCG+TGG 0.686464 5_1:-65224163 Msa0744440:CDS
AAGAACTAGAGAGAATTGTG+AGG 0.696370 5_1:-65223424 Msa0744440:CDS
GAAACATGCACCAACAACAG+TGG 0.702295 5_1:-65224493 Msa0744440:CDS
TGGACTTCGAGTTATCCTTG+AGG 0.704359 5_1:-65224776 Msa0744440:CDS
GGATCCTCAGGAGACAAGCG+AGG 0.706361 5_1:+65225057 None:intergenic
AGTACCTCTTCCATTCACAG+AGG 0.714389 5_1:+65222953 None:intergenic
AAAAGAGGTTCATCCCACGA+TGG 0.725729 5_1:+65224149 None:intergenic
AATACATCAGCATTGAAGAG+GGG 0.734552 5_1:+65223177 None:intergenic
TTTCAGTAGGTGATTCACGG+AGG 0.740333 5_1:+65225016 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTCATTAAAAAAACTAAATT+AGG - chr5_1:65224026-65224045 Msa0744440:intron 10.0%
!! TTAATTTAAACTTGAAAATT+AGG - chr5_1:65225093-65225112 Msa0744440:CDS 10.0%
!! ATTAATTATAAGCACTTAAT+AGG + chr5_1:65225078-65225097 None:intergenic 15.0%
!! AAAACTGAAAAGTAGAAAAA+TGG - chr5_1:65223832-65223851 Msa0744440:intron 20.0%
!!! ATTCGTATGTGTTTAAAATT+GGG + chr5_1:65224106-65224125 None:intergenic 20.0%
!!! TATTCGTATGTGTTTAAAAT+TGG + chr5_1:65224107-65224126 None:intergenic 20.0%
!! GAATGAGGATAAATAAATAA+AGG + chr5_1:65224212-65224231 None:intergenic 20.0%
!!! AATTGTTTTATATTAGAGCC+TGG + chr5_1:65222776-65222795 None:intergenic 25.0%
! GCTCTAATATAAAACAATTG+TGG - chr5_1:65222777-65222796 Msa0744440:three_prime_UTR 25.0%
! TAGAAATTACAACCAACAAT+TGG + chr5_1:65224882-65224901 None:intergenic 25.0%
! ACTCTTTGTCAATTGTATAA+CGG + chr5_1:65224929-65224948 None:intergenic 25.0%
! TTATACAATTGACAAAGAGT+TGG - chr5_1:65224929-65224948 Msa0744440:CDS 25.0%
!! ATAAGTTGAAATTCGAAACT+AGG - chr5_1:65225017-65225036 Msa0744440:CDS 25.0%
! ATCTTTGTCTCTTGTTTCTT+CGG - chr5_1:65222854-65222873 Msa0744440:three_prime_UTR 30.0%
TTTGTTAGTAGGATCTTTCA+TGG - chr5_1:65223755-65223774 Msa0744440:intron 30.0%
!! GCAATATTGACTGTTTTTCA+AGG - chr5_1:65223875-65223894 Msa0744440:intron 30.0%
! TATCTGCATTGACATAATTG+TGG - chr5_1:65223938-65223957 Msa0744440:intron 30.0%
CTCACTTTCTATATCACATT+TGG - chr5_1:65223982-65224001 Msa0744440:intron 30.0%
TTTGGTTATGTCTGAACAAA+TGG - chr5_1:65224000-65224019 Msa0744440:intron 30.0%
!! GAAAAGCATTTGTTAGAATG+AGG + chr5_1:65224227-65224246 None:intergenic 30.0%
! TTTGAAACAGAAGTTAAGTG+AGG - chr5_1:65224660-65224679 Msa0744440:CDS 30.0%
GTATGAGAAAATCAAATTGC+AGG - chr5_1:65224705-65224724 Msa0744440:CDS 30.0%
! TGTTTTATATTAGAGCCTGG+TGG + chr5_1:65222773-65222792 None:intergenic 35.0%
CTAAAAATTCACCAGGTAGA+CGG + chr5_1:65223127-65223146 None:intergenic 35.0%
!! TCTACCTGGTGAATTTTTAG+AGG - chr5_1:65223127-65223146 Msa0744440:CDS 35.0%
!! TTTTTAGAGGAGCTGAAAAC+TGG - chr5_1:65223140-65223159 Msa0744440:CDS 35.0%
ATCCTTGTTGCCATAAATAC+TGG - chr5_1:65223296-65223315 Msa0744440:CDS 35.0%
TTCCAGTATTTATGGCAACA+AGG + chr5_1:65223301-65223320 None:intergenic 35.0%
TGCCATAAATACTGGAATTC+AGG - chr5_1:65223304-65223323 Msa0744440:CDS 35.0%
GCCATAAATACTGGAATTCA+GGG - chr5_1:65223305-65223324 Msa0744440:CDS 35.0%
TCCCTGAATTCCAGTATTTA+TGG + chr5_1:65223309-65223328 None:intergenic 35.0%
AGAAATATCTCAATCAGCGA+TGG + chr5_1:65223362-65223381 None:intergenic 35.0%
TTGAAGTAAATACATGCCGT+TGG - chr5_1:65223510-65223529 Msa0744440:CDS 35.0%
ACAAATCACATCCAATCCAA+CGG + chr5_1:65223529-65223548 None:intergenic 35.0%
! GATGTGATTTGTGTTCTCAT+TGG - chr5_1:65223537-65223556 Msa0744440:CDS 35.0%
! TTCCACTTTCTACATTTTCC+AGG + chr5_1:65224285-65224304 None:intergenic 35.0%
TGGAAAATGTAGAAAGTGGA+AGG - chr5_1:65224284-65224303 Msa0744440:CDS 35.0%
GAAGATGAGAATGTTCAAGA+AGG - chr5_1:65224390-65224409 Msa0744440:CDS 35.0%
AAGAACTAGAGAGAATTGTG+AGG - chr5_1:65224512-65224531 Msa0744440:CDS 35.0%
TCTTGCGAAGTCTGATAAAT+CGG - chr5_1:65224615-65224634 Msa0744440:CDS 35.0%
CTTCTGTTTCAAATAGTTGC+TGG + chr5_1:65224653-65224672 None:intergenic 35.0%
! AATACATCAGCATTGAAGAG+GGG + chr5_1:65224762-65224781 None:intergenic 35.0%
! GAATACATCAGCATTGAAGA+GGG + chr5_1:65224763-65224782 None:intergenic 35.0%
! GTAAAGGAGATCATGGATTT+TGG + chr5_1:65224785-65224804 None:intergenic 35.0%
ATCCATGATCTCCTTTACAA+TGG - chr5_1:65224787-65224806 Msa0744440:CDS 35.0%
TTAGATTGACTGTCTGCTTT+GGG + chr5_1:65224817-65224836 None:intergenic 35.0%
ATTAGATTGACTGTCTGCTT+TGG + chr5_1:65224818-65224837 None:intergenic 35.0%
AATTGGACCAATACTCATCA+CGG + chr5_1:65224865-65224884 None:intergenic 35.0%
!! ATGAGTATTGGTCCAATTGT+TGG - chr5_1:65224867-65224886 Msa0744440:CDS 35.0%
TGGTTCAATTCCATTGCATT+TGG - chr5_1:65224949-65224968 Msa0744440:CDS 35.0%
GGTTCAATTCCATTGCATTT+GGG - chr5_1:65224950-65224969 Msa0744440:CDS 35.0%
GTTCAATTCCATTGCATTTG+GGG - chr5_1:65224951-65224970 Msa0744440:CDS 35.0%
TGTTCTATGCAGTGTTTGTA+TGG - chr5_1:65225042-65225061 Msa0744440:CDS 35.0%
AGGTTATCAGAGTTTGACCT+AGG + chr5_1:65222903-65222922 None:intergenic 40.0%
CACTTTCAGTAGGTGATTCA+CGG + chr5_1:65222926-65222945 None:intergenic 40.0%
CTAGAACTAGCACTTTCAGT+AGG + chr5_1:65222936-65222955 None:intergenic 40.0%
!! TATTCGTCGTGTTTCTAGTG+CGG - chr5_1:65223040-65223059 Msa0744440:three_prime_UTR 40.0%
! AGCTCCTCTAAAAATTCACC+AGG + chr5_1:65223134-65223153 None:intergenic 40.0%
ATGAGCTCGAATCAATGTCT+TGG + chr5_1:65223261-65223280 None:intergenic 40.0%
!! AGACATTGATTCGAGCTCAT+CGG - chr5_1:65223261-65223280 Msa0744440:CDS 40.0%
!! GGTTTTGGCAAGCTATGTTT+CGG + chr5_1:65223397-65223416 None:intergenic 40.0%
TCACAAGAACAATCATCAGC+TGG + chr5_1:65223425-65223444 None:intergenic 40.0%
GTAAATACATGCCGTTGGAT+TGG - chr5_1:65223515-65223534 Msa0744440:CDS 40.0%
! TGCCAAGTGAAATGGTTTTC+AGG - chr5_1:65223627-65223646 Msa0744440:CDS 40.0%
CACCTGAAAACCATTTCACT+TGG + chr5_1:65223632-65223651 None:intergenic 40.0%
AGTAGGACTTCAGAACTGTT+AGG - chr5_1:65223662-65223681 Msa0744440:CDS 40.0%
AAAATCAAGCTCCCTCATCA+AGG + chr5_1:65223738-65223757 None:intergenic 40.0%
! GGGAGCTTGATTTTGTTAGT+AGG - chr5_1:65223744-65223763 Msa0744440:intron 40.0%
ATGGTAGTAAAGACCATCGT+GGG - chr5_1:65223774-65223793 Msa0744440:intron 40.0%
! TCTACATTTTCCAGGCTCTT+TGG + chr5_1:65224277-65224296 None:intergenic 40.0%
AGCCTGGAAAATGTAGAAAG+TGG - chr5_1:65224280-65224299 Msa0744440:CDS 40.0%
ATGCAACAGAATTGCTGAAG+TGG - chr5_1:65224327-65224346 Msa0744440:CDS 40.0%
AGAAGCAGAAGAACTCAAGA+TGG - chr5_1:65224462-65224481 Msa0744440:CDS 40.0%
CAATTGGAACATATCAGCCT+CGG + chr5_1:65224548-65224567 None:intergenic 40.0%
GGCTGATATGTTCCAATTGA+AGG - chr5_1:65224549-65224568 Msa0744440:CDS 40.0%
GTTCCAATTGAAGGCTAATG+AGG - chr5_1:65224558-65224577 Msa0744440:CDS 40.0%
! GGAATACATCAGCATTGAAG+AGG + chr5_1:65224764-65224783 None:intergenic 40.0%
ATTGGACCAATACTCATCAC+GGG + chr5_1:65224864-65224883 None:intergenic 40.0%
TTATAGTGCCCCAAATGCAA+TGG + chr5_1:65224962-65224981 None:intergenic 40.0%
GCACTATAAACCTCTGTGAA+TGG - chr5_1:65224973-65224992 Msa0744440:CDS 40.0%
TAAACCTCTGTGAATGGAAG+AGG - chr5_1:65224979-65224998 Msa0744440:CDS 40.0%
CAGTGTTTGTATGGATATGC+TGG - chr5_1:65225051-65225070 Msa0744440:CDS 40.0%
TATTCTACGCAGTGTATGCA+TGG - chr5_1:65225118-65225137 Msa0744440:CDS 40.0%
TTTCAGTAGGTGATTCACGG+AGG + chr5_1:65222923-65222942 None:intergenic 45.0%
!! GTGCTAGTTCTAGAGAAACC+TGG - chr5_1:65222943-65222962 Msa0744440:three_prime_UTR 45.0%
CGACGAATAACTGACTGATC+AGG + chr5_1:65223029-65223048 None:intergenic 45.0%
TCAGTCTTCAGGACATTGCT+CGG - chr5_1:65223069-65223088 Msa0744440:three_prime_UTR 45.0%
GAAAAAATGCTCCGTCTACC+TGG - chr5_1:65223113-65223132 Msa0744440:CDS 45.0%
! TGGACTTCGAGTTATCCTTG+AGG - chr5_1:65223160-65223179 Msa0744440:CDS 45.0%
! GGACTTCGAGTTATCCTTGA+GGG - chr5_1:65223161-65223180 Msa0744440:CDS 45.0%
! TTCGAGTTATCCTTGAGGGA+GGG - chr5_1:65223165-65223184 Msa0744440:CDS 45.0%
!! GACATTGATTCGAGCTCATC+GGG - chr5_1:65223262-65223281 Msa0744440:CDS 45.0%
AACAATCATCAGCTGGAAGC+TGG + chr5_1:65223418-65223437 None:intergenic 45.0%
GAAACATGCACCAACAACAG+TGG - chr5_1:65223443-65223462 Msa0744440:CDS 45.0%
ATTGCAGAAGCCACTGTTGT+TGG + chr5_1:65223456-65223475 None:intergenic 45.0%
CTCACACAGATTGTGCCATA+CGG - chr5_1:65223561-65223580 Msa0744440:CDS 45.0%
TCACACAGATTGTGCCATAC+GGG - chr5_1:65223562-65223581 Msa0744440:CDS 45.0%
GCAAATAAGTTGCTCCCGTA+TGG + chr5_1:65223579-65223598 None:intergenic 45.0%
ACGGGAGCAACTTATTTGCA+TGG - chr5_1:65223580-65223599 Msa0744440:CDS 45.0%
CGGGAGCAACTTATTTGCAT+GGG - chr5_1:65223581-65223600 Msa0744440:CDS 45.0%
ACTGATCCACTCTTTACAGG+AGG + chr5_1:65223608-65223627 None:intergenic 45.0%
TGGATCAGTGCCAAGTGAAA+TGG - chr5_1:65223619-65223638 Msa0744440:CDS 45.0%
TCAGGTGTCAATCATGCAGT+AGG - chr5_1:65223645-65223664 Msa0744440:CDS 45.0%
GACTTCAGAACTGTTAGGCT+GGG - chr5_1:65223667-65223686 Msa0744440:CDS 45.0%
TCCAACATTGTGCACCATCA+TGG - chr5_1:65223702-65223721 Msa0744440:intron 45.0%
CCAACATTGTGCACCATCAT+GGG - chr5_1:65223703-65223722 Msa0744440:intron 45.0%
ATTGTGCACCATCATGGGAT+GGG - chr5_1:65223708-65223727 Msa0744440:intron 45.0%
CATGGTAGTAAAGACCATCG+TGG - chr5_1:65223773-65223792 Msa0744440:intron 45.0%
AAAAGAGGTTCATCCCACGA+TGG + chr5_1:65223790-65223809 None:intergenic 45.0%
! ATCGTGGGATGAACCTCTTT+TGG - chr5_1:65223789-65223808 Msa0744440:intron 45.0%
GATCGTCACACTTCCAAAAG+AGG + chr5_1:65223805-65223824 None:intergenic 45.0%
! TTGGAAGTGTGACGATCTCA+AGG - chr5_1:65223808-65223827 Msa0744440:intron 45.0%
AAAGGCAACAGCAGCATATC+TGG + chr5_1:65224194-65224213 None:intergenic 45.0%
CTAGGTCAAGCTCTTCATGT+AGG + chr5_1:65224249-65224268 None:intergenic 45.0%
TGATCGTGAACTAGCAGACA+AGG - chr5_1:65224435-65224454 Msa0744440:CDS 45.0%
CTTGAGTTCTTCTGCTTCTC+TGG + chr5_1:65224461-65224480 None:intergenic 45.0%
CAGAAGAACTCAAGATGGAG+AGG - chr5_1:65224467-65224486 Msa0744440:CDS 45.0%
TGTGAGGCTTAAAAATGCCG+AGG - chr5_1:65224528-65224547 Msa0744440:CDS 45.0%
!! TGGCCTCATTAGCCTTCAAT+TGG + chr5_1:65224564-65224583 None:intergenic 45.0%
ACAGAAGTTAAGTGAGGCTG+AGG - chr5_1:65224666-65224685 Msa0744440:CDS 45.0%
GGCCATTGTAAAGGAGATCA+TGG + chr5_1:65224792-65224811 None:intergenic 45.0%
!! ATTGACTGTCTGCTTTGGGA+GGG + chr5_1:65224813-65224832 None:intergenic 45.0%
ATACTCATCACGGGTTTGTC+CGG + chr5_1:65224855-65224874 None:intergenic 45.0%
GACAAACCCGTGATGAGTAT+TGG - chr5_1:65224855-65224874 Msa0744440:CDS 45.0%
AGTACCTCTTCCATTCACAG+AGG + chr5_1:65224986-65225005 None:intergenic 45.0%
TTCATGGACATGTCACCACC+AGG - chr5_1:65222755-65222774 Msa0744440:three_prime_UTR 50.0%
ATGTTTGCAGTCCTCCAACC+CGG + chr5_1:65222836-65222855 None:intergenic 50.0%
GAGTTTGACCTAGGATCCTC+AGG + chr5_1:65222894-65222913 None:intergenic 50.0%
TGCTATCGCTGCGAAGAAGA+TGG - chr5_1:65222977-65222996 Msa0744440:three_prime_UTR 50.0%
GCTGCGAAGAAGATGGAGAA+TGG - chr5_1:65222984-65223003 Msa0744440:three_prime_UTR 50.0%
TGCGGACAGAATCAGTCTTC+AGG - chr5_1:65223058-65223077 Msa0744440:three_prime_UTR 50.0%
CAGTCTTCAGGACATTGCTC+GGG - chr5_1:65223070-65223089 Msa0744440:three_prime_UTR 50.0%
! CTTCGAGTTATCCTTGAGGG+AGG - chr5_1:65223164-65223183 Msa0744440:CDS 50.0%
GTTATCCTTGAGGGAGGGAA+TGG - chr5_1:65223170-65223189 Msa0744440:CDS 50.0%
GGCTGCGACAGACTAATGTT+AGG + chr5_1:65223341-65223360 None:intergenic 50.0%
!! CATCAGCTGGAAGCTGGTTT+TGG + chr5_1:65223412-65223431 None:intergenic 50.0%
ATGGGTCCTCCTGTAAAGAG+TGG - chr5_1:65223599-65223618 Msa0744440:CDS 50.0%
!! GGCACTGATCCACTCTTTAC+AGG + chr5_1:65223611-65223630 None:intergenic 50.0%
GGACTTCAGAACTGTTAGGC+TGG - chr5_1:65223666-65223685 Msa0744440:CDS 50.0%
! CCCATGATGGTGCACAATGT+TGG + chr5_1:65223706-65223725 None:intergenic 50.0%
CATTGTGCACCATCATGGGA+TGG - chr5_1:65223707-65223726 Msa0744440:intron 50.0%
TTGTGCACCATCATGGGATG+GGG - chr5_1:65223709-65223728 Msa0744440:intron 50.0%
AAGGAATCCCCATCCCATGA+TGG + chr5_1:65223719-65223738 None:intergenic 50.0%
GGATGGGGATTCCTTGATGA+GGG - chr5_1:65223724-65223743 Msa0744440:intron 50.0%
TGGAAGCTAAAGCAGCATGC+AGG - chr5_1:65223852-65223871 Msa0744440:intron 50.0%
GGAAGCTAAAGCAGCATGCA+GGG - chr5_1:65223853-65223872 Msa0744440:intron 50.0%
! TGGAAGGTTGATTGCTCCAC+AGG - chr5_1:65224300-65224319 Msa0744440:CDS 50.0%
! AAGGTTGATTGCTCCACAGG+AGG - chr5_1:65224303-65224322 Msa0744440:CDS 50.0%
ATTCTGTTGCATGCCTCCTG+TGG + chr5_1:65224319-65224338 None:intergenic 50.0%
CAGAATTGCTGAAGTGGTGC+AGG - chr5_1:65224333-65224352 Msa0744440:CDS 50.0%
AATTGCTGAAGTGGTGCAGG+AGG - chr5_1:65224336-65224355 Msa0744440:CDS 50.0%
GCAGGAGGCTATACGAAAGA+TGG - chr5_1:65224351-65224370 Msa0744440:CDS 50.0%
ACTTCGCAAGAGCAATCCTC+TGG + chr5_1:65224606-65224625 None:intergenic 50.0%
! CTTTGGGAGGGCCATTGTAA+AGG + chr5_1:65224801-65224820 None:intergenic 50.0%
! GATTGACTGTCTGCTTTGGG+AGG + chr5_1:65224814-65224833 None:intergenic 50.0%
CTAATGACTGCCACCCTTTC+CGG - chr5_1:65224833-65224852 Msa0744440:CDS 50.0%
ATCACGGGTTTGTCCGGAAA+GGG + chr5_1:65224849-65224868 None:intergenic 50.0%
TGTTTGCAGTCCTCCAACCC+GGG + chr5_1:65222835-65222854 None:intergenic 55.0%
GCTTGTCTCCTGAGGATCCT+AGG - chr5_1:65222883-65222902 Msa0744440:three_prime_UTR 55.0%
GATAGCATCAGCAGTAGGCC+AGG + chr5_1:65222964-65222983 None:intergenic 55.0%
GCAGCGATAGCATCAGCAGT+AGG + chr5_1:65222969-65222988 None:intergenic 55.0%
GGACATTGCTCGGGAAAGAG+TGG - chr5_1:65223079-65223098 Msa0744440:exon 55.0%
GGGATGGGGATTCCTTGATG+AGG - chr5_1:65223723-65223742 Msa0744440:intron 55.0%
! CCAGGCTCTTTGGAGAGTCT+AGG + chr5_1:65224267-65224286 None:intergenic 55.0%
CCTAGACTCTCCAAAGAGCC+TGG - chr5_1:65224264-65224283 Msa0744440:CDS 55.0%
GAAGGCTCGCTTAGCTGTTG+AGG - chr5_1:65224408-65224427 Msa0744440:CDS 55.0%
GGCTAATGAGGCCAAACGAG+AGG - chr5_1:65224570-65224589 Msa0744440:CDS 55.0%
TCTCGCTTATCGCAGAGCAG+TGG - chr5_1:65224733-65224752 Msa0744440:CDS 55.0%
CATCACGGGTTTGTCCGGAA+AGG + chr5_1:65224850-65224869 None:intergenic 55.0%
TCCCTCCACGACAGCAATCC+CGG - chr5_1:65222814-65222833 Msa0744440:three_prime_UTR 60.0%
GGATCCTCGCTTGTCTCCTG+AGG - chr5_1:65222875-65222894 Msa0744440:three_prime_UTR 60.0%
GGATCCTCAGGAGACAAGCG+AGG + chr5_1:65222882-65222901 None:intergenic 60.0%
GCATGCCATTCCCTCCCTCA+AGG + chr5_1:65223178-65223197 None:intergenic 60.0%
AGGCCAAACGAGAGGCTGAG+AGG - chr5_1:65224578-65224597 Msa0744440:CDS 60.0%
GAGCCTCTCAGCCTCTCGTT+TGG + chr5_1:65224584-65224603 None:intergenic 60.0%
ACGGGTTTGTCCGGAAAGGG+TGG + chr5_1:65224846-65224865 None:intergenic 60.0%
CCCGGGATTGCTGTCGTGGA+GGG + chr5_1:65222818-65222837 None:intergenic 65.0%
CCCTCCACGACAGCAATCCC+GGG - chr5_1:65222815-65222834 Msa0744440:three_prime_UTR 65.0%
ACCCGGGATTGCTGTCGTGG+AGG + chr5_1:65222819-65222838 None:intergenic 65.0%
CCAACCCGGGATTGCTGTCG+TGG + chr5_1:65222822-65222841 None:intergenic 65.0%
CCACGACAGCAATCCCGGGT+TGG - chr5_1:65222819-65222838 Msa0744440:three_prime_UTR 65.0%
! CGACAGCAATCCCGGGTTGG+AGG - chr5_1:65222822-65222841 Msa0744440:three_prime_UTR 65.0%
GAGAGGCTGAGAGGCTCCAG+AGG - chr5_1:65224587-65224606 Msa0744440:CDS 65.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 65222752 65225206 65222752 ID=Msa0744440;Name=Msa0744440
chr5_1 mRNA 65222752 65225206 65222752 ID=Msa0744440-mRNA-1;Parent=Msa0744440;Name=Msa0744440-mRNA-1;_AED=0.01;_eAED=0.01;_QI=58|1|1|1|1|1|2|339|565
chr5_1 exon 65222752 65223701 65222752 ID=Msa0744440-mRNA-1:exon:19358;Parent=Msa0744440-mRNA-1
chr5_1 exon 65224062 65225206 65224062 ID=Msa0744440-mRNA-1:exon:19357;Parent=Msa0744440-mRNA-1
chr5_1 five_prime_UTR 65225149 65225206 65225149 ID=Msa0744440-mRNA-1:five_prime_utr;Parent=Msa0744440-mRNA-1
chr5_1 CDS 65224062 65225148 65224062 ID=Msa0744440-mRNA-1:cds;Parent=Msa0744440-mRNA-1
chr5_1 CDS 65223091 65223701 65223091 ID=Msa0744440-mRNA-1:cds;Parent=Msa0744440-mRNA-1
chr5_1 three_prime_UTR 65222752 65223090 65222752 ID=Msa0744440-mRNA-1:three_prime_utr;Parent=Msa0744440-mRNA-1
Gene Sequence

>Msa0744440

ATGCTCCCTCCACGACAGCAATCCCGGGTTGGAGGACTGCAAACATCTTTGTCTCTTGTTTCTTCGGATCCTCGCTTGTCTCCTGAGGATCCTAGGTCAAACTCTGATAACCTCCGTGAATCACCTACTGAAAGTGCTAGTTCTAGAGAAACCTGGCCTACTGCTGATGCTATCGCTGCGAAGAAGATGGAGAATGGAAAAGCTGATATTGACTGTCCTGATCAGTCAGTTATTCGTCGTGTTTCTAGTGCGGACAGAATCAGTCTTCAGGACATTGCTCGGGAAAGAGTGGATGTAATACATGAAAAAATGCTCCGTCTACCTGGTGAATTTTTAGAGGAGCTGAAAACTGGACTTCGAGTTATCCTTGAGGGAGGGAATGGCATGCAGCATAGAGATGAGTTTTTCATTTTGCAGAAGCTTGTTCAGAGTAGATCTGATTTGACAGCCAAGACATTGATTCGAGCTCATCGGGTGCAGCTTGAAATCCTTGTTGCCATAAATACTGGAATTCAGGGATTTTTACATCCTAACATTAGTCTGTCGCAGCCATCGCTGATTGAGATATTTCTCTACAAGAGATGCCGAAACATAGCTTGCCAAAACCAGCTTCCAGCTGATGATTGTTCTTGTGAAACATGCACCAACAACAGTGGCTTCTGCAATCTTTGCATGTGTACTATCTGCAGCAAGTTTGACTTTGAAGTAAATACATGCCGTTGGATTGGATGTGATTTGTGTTCTCATTGGACTCACACAGATTGTGCCATACGGGAGCAACTTATTTGCATGGGTCCTCCTGTAAAGAGTGGATCAGTGCCAAGTGAAATGGTTTTCAGGTGTCAATCATGCAGTAGGACTTCAGAACTGTTAGGCTGGGTTAAAGATGTCTTCCAACATTGTGCACCATCATGGGATGGGGATTCCTTGATGAGGGAGCTTGATTTTGTTAGTAGGATCTTTCATGGTAGTAAAGACCATCGTGGGATGAACCTCTTTTGGAAGTGTGACGATCTCAAGGAAAAACTGAAAAGTAGAAAAATGGAAGCTAAAGCAGCATGCAGGGCAATATTGACTGTTTTTCAAGAGCTTGACCTAGACTCTCCAAAGAGCCTGGAAAATGTAGAAAGTGGAAGGTTGATTGCTCCACAGGAGGCATGCAACAGAATTGCTGAAGTGGTGCAGGAGGCTATACGAAAGATGGAATTTGTAGCTGATGAGAAGATGAGAATGTTCAAGAAGGCTCGCTTAGCTGTTGAGGCTTGTGATCGTGAACTAGCAGACAAGGCCAGAGAAGCAGAAGAACTCAAGATGGAGAGGCAAAAAAAGAAGTCACAGATTGAAGAACTAGAGAGAATTGTGAGGCTTAAAAATGCCGAGGCTGATATGTTCCAATTGAAGGCTAATGAGGCCAAACGAGAGGCTGAGAGGCTCCAGAGGATTGCTCTTGCGAAGTCTGATAAATCGGAAGAAGAATATACCAGCAACTATTTGAAACAGAAGTTAAGTGAGGCTGAGGCTGAGAAGCAATATCTGTATGAGAAAATCAAATTGCAGGAGAGTTCTCGCTTATCGCAGAGCAGTGGTGACCCCTCTTCAATGCTGATGTATTCCAAAATCCATGATCTCCTTTACAATGGCCCTCCCAAAGCAGACAGTCAATCTAATGACTGCCACCCTTTCCGGACAAACCCGTGA
Protein sequence

>Msa0744440

MLPPRQQSRVGGLQTSLSLVSSDPRLSPEDPRSNSDNLRESPTESASSRETWPTADAIAAKKMENGKADIDCPDQSVIRRVSSADRISLQDIARERVDVIHEKMLRLPGEFLEELKTGLRVILEGGNGMQHRDEFFILQKLVQSRSDLTAKTLIRAHRVQLEILVAINTGIQGFLHPNISLSQPSLIEIFLYKRCRNIACQNQLPADDCSCETCTNNSGFCNLCMCTICSKFDFEVNTCRWIGCDLCSHWTHTDCAIREQLICMGPPVKSGSVPSEMVFRCQSCSRTSELLGWVKDVFQHCAPSWDGDSLMRELDFVSRIFHGSKDHRGMNLFWKCDDLKEKLKSRKMEAKAACRAILTVFQELDLDSPKSLENVESGRLIAPQEACNRIAEVVQEAIRKMEFVADEKMRMFKKARLAVEACDRELADKAREAEELKMERQKKKSQIEELERIVRLKNAEADMFQLKANEAKREAERLQRIALAKSDKSEEEYTSNYLKQKLSEAEAEKQYLYEKIKLQESSRLSQSSGDPSSMLMYSKIHDLLYNGPPKADSQSNDCHPFRTNP*