AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0744480

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0744480 MtrunA17_Chr7g0252171 55.446 101 22 1 96 173 364 464 4.58e-25 101
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 73 sgRNAs with CRISPR-Local

Find 151 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCATCTCCACTTGCTTTAT+TGG 0.176059 5_1:-65281947 Msa0744480:CDS
CTTCCTTCTTATCTCATAAA+TGG 0.193129 5_1:-65281676 Msa0744480:three_prime_UTR
TAATACATCAGGTGACTTAT+TGG 0.198252 5_1:-65281286 Msa0744480:three_prime_UTR
TATAATTTGATAGATATTAT+TGG 0.208885 5_1:-65281733 Msa0744480:three_prime_UTR
TAATCAGCAGTAACATTATT+TGG 0.248198 5_1:+65282094 None:intergenic
GGTGAAGTGGCTAACTTCTT+TGG 0.252456 5_1:-65281368 Msa0744480:three_prime_UTR
CTCATAAATGGATGCACTAA+TGG 0.290935 5_1:-65281664 Msa0744480:three_prime_UTR
GAGAGAGAGTGAGAAGGTTC+TGG 0.296791 5_1:+65283440 None:intergenic
AAGAAAGGAACAAAGATATA+TGG 0.298600 5_1:+65281317 None:intergenic
ACTAACATAACGGTCATTAT+TGG 0.298778 5_1:+65281072 None:intergenic
GAAATCATATAGGATGAAAA+AGG 0.331410 5_1:+65281783 None:intergenic
TCACATGCGAACTTGATCTT+TGG 0.334210 5_1:+65281920 None:intergenic
TCCTCCAATGAGTGCGCTAA+TGG 0.353003 5_1:-65281636 Msa0744480:three_prime_UTR
TATGAAAATCAGATTTCAAT+AGG 0.353141 5_1:-65282073 Msa0744480:CDS
AAATATGTCAAAACATATAT+AGG 0.355946 5_1:-65281397 Msa0744480:three_prime_UTR
GAGTGTTGGAACAGAAGTTC+TGG 0.357889 5_1:+65283353 None:intergenic
CAAAACATATATAGGATTCC+TGG 0.384126 5_1:-65281389 Msa0744480:three_prime_UTR
CATGTGATAAACACAGTAAC+AGG 0.403502 5_1:-65281904 Msa0744480:CDS
TTTAAAGGGAGGAATGAATC+AGG 0.405554 5_1:-65282118 Msa0744480:CDS
ATAAAATAACAAACTTCATC+AGG 0.411509 5_1:+65281219 None:intergenic
TGAACTTCTGATGCAATTGT+AGG 0.413663 5_1:-65281095 Msa0744480:three_prime_UTR
CTTTGACGAAATAATACATC+AGG 0.421986 5_1:-65281297 Msa0744480:three_prime_UTR
GAGTGAGAAGGTTCTGGAGA+TGG 0.425661 5_1:+65283446 None:intergenic
GAGTGAGAAAGAGAGGTTCC+TGG 0.427374 5_1:+65283407 None:intergenic
TATTTAGGCAGCAAGGCAAT+TGG 0.434701 5_1:-65282146 Msa0744480:intron
AGAAAGGAACAAAGATATAT+GGG 0.447690 5_1:+65281318 None:intergenic
TGTATTATTTCGTCAAAGAA+AGG 0.463318 5_1:+65281302 None:intergenic
GTCTCCATTAGCGCACTCAT+TGG 0.465837 5_1:+65281632 None:intergenic
TAATTTGCAGGCAGAAGGCT+TGG 0.477127 5_1:-65281971 Msa0744480:intron
CACAGTAACAGGCTTCCAAC+AGG 0.481101 5_1:-65281893 Msa0744480:CDS
CTCAAAGTAATATTAGATAT+AGG 0.489671 5_1:+65281431 None:intergenic
TCTCTCTCCCTCACAGCTCC+AGG 0.492855 5_1:-65283425 Msa0744480:CDS
CATCCATTTATGAGATAAGA+AGG 0.498458 5_1:+65281673 None:intergenic
AGAGGTTCCTGGAGCTGTGA+GGG 0.500020 5_1:+65283418 None:intergenic
AAACACATACACATTAGTTA+TGG 0.511165 5_1:-65281608 Msa0744480:intron
TCCATTAGCGCACTCATTGG+AGG 0.514548 5_1:+65281635 None:intergenic
TTTCTTAATTTGCAGGCAGA+AGG 0.528909 5_1:-65281976 Msa0744480:intron
TGTCTAGAACTCTTCTGGGA+TGG 0.529191 5_1:-65281474 Msa0744480:three_prime_UTR
TGAGGGAGACAGTGATGGAA+TGG 0.541483 5_1:+65283226 None:intergenic
GCCACAGGTTTCCACTGCAA+AGG 0.543513 5_1:-65281501 Msa0744480:intron
GACAACGAAGAGAGTGACAG+AGG 0.548492 5_1:+65283322 None:intergenic
TTCTTGATATTTAGGCAGCA+AGG 0.550166 5_1:-65282153 Msa0744480:intron
AAGAAGTTAGCCACTTCACC+AGG 0.558942 5_1:+65281371 None:intergenic
AGAGAGTGAGAGTGATGGAG+CGG 0.564177 5_1:+65283270 None:intergenic
CTCTATGAGGGAGACAGTGA+TGG 0.564661 5_1:+65283221 None:intergenic
CAGAGGGAGAGAGTGAGTGT+TGG 0.569370 5_1:+65283339 None:intergenic
ACCTCTCTTTCTCACTCTCA+CGG 0.570435 5_1:-65283401 Msa0744480:CDS
ATATAGGATTCCTGGTGAAG+TGG 0.572171 5_1:-65281381 Msa0744480:three_prime_UTR
GAGGGAGAGAGTGAGAGTGA+TGG 0.573929 5_1:+65283265 None:intergenic
TGGTTTACCAATAAAGCAAG+TGG 0.574147 5_1:+65281940 None:intergenic
ATGAAGTGAATCAAAGGAGA+GGG 0.586945 5_1:+65283510 None:intergenic
GCCGTGAGAGTGAGAAAGAG+AGG 0.594932 5_1:+65283400 None:intergenic
TTAGCGCACTCATTGGAGGA+AGG 0.595443 5_1:+65281639 None:intergenic
TAGCGCACTCATTGGAGGAA+GGG 0.596922 5_1:+65281640 None:intergenic
TTATTGGAACTTACTGACCA+TGG 0.600702 5_1:-65281717 Msa0744480:three_prime_UTR
AGTGACGGCGGCGAGTGAGA+CGG 0.601405 5_1:+65283378 None:intergenic
CATCACTGTCTCCCTCATAG+AGG 0.602023 5_1:-65283220 Msa0744480:intron
TGCAACGGCTACAGTAACAG+AGG 0.604475 5_1:+65283295 None:intergenic
AAAGCGTGAAACTAACATAA+CGG 0.608728 5_1:+65281062 None:intergenic
TATGACTAGAGTGCACCTGT+TGG 0.613837 5_1:+65281878 None:intergenic
AGTGATGGAGCGGTTTGCAA+CGG 0.620178 5_1:+65283280 None:intergenic
ACAAAGCATATAAGTTACCA+TGG 0.622412 5_1:+65281700 None:intergenic
ACAACGAAGAGAGTGACAGA+GGG 0.627741 5_1:+65283323 None:intergenic
GAATGGAACGATGAGAGTGA+CGG 0.633619 5_1:+65283243 None:intergenic
GTGAGGGAGAGAGAGTGAGA+AGG 0.635071 5_1:+65283434 None:intergenic
GAGAGGTTCCTGGAGCTGTG+AGG 0.635130 5_1:+65283417 None:intergenic
TGGAACGATGAGAGTGACGG+AGG 0.636331 5_1:+65283246 None:intergenic
GCAACGGCTACAGTAACAGA+GGG 0.637006 5_1:+65283296 None:intergenic
AATGAAGTGAATCAAAGGAG+AGG 0.637383 5_1:+65283509 None:intergenic
ACAGAAGTTCTGGAGAGTGA+CGG 0.642423 5_1:+65283363 None:intergenic
GGAACGATGAGAGTGACGGA+GGG 0.642773 5_1:+65283247 None:intergenic
GATTTCAATAGGCTATGATG+AGG 0.681055 5_1:-65282062 Msa0744480:intron
GAAGTTCTGGAGAGTGACGG+CGG 0.708666 5_1:+65283366 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCATAAATCAATTTTTTATT+TGG - chr5_1:65281401-65281420 Msa0744480:three_prime_UTR 10.0%
!!! CATAAATCAATTTTTTATTT+GGG - chr5_1:65281402-65281421 Msa0744480:three_prime_UTR 10.0%
!! AAAAAAATAAGAAATCATAT+AGG + chr5_1:65282811-65282830 None:intergenic 10.0%
!! TATAATTTGATAGATATTAT+TGG - chr5_1:65282848-65282867 Msa0744480:intron 10.0%
!!! AATTTTTTATTTGGGAATTA+GGG - chr5_1:65281410-65281429 Msa0744480:three_prime_UTR 15.0%
!! TTGATGAAAAATCAATATTT+AGG - chr5_1:65281504-65281523 Msa0744480:intron 15.0%
!! TGATGAAAAATCAATATTTA+GGG - chr5_1:65281505-65281524 Msa0744480:intron 15.0%
!!! TTACAATTTAAGACAATTTA+AGG - chr5_1:65281550-65281569 Msa0744480:intron 15.0%
!! AACAATAGACATAATAATAT+TGG + chr5_1:65281825-65281844 None:intergenic 15.0%
!! AAATATGTCAAAACATATAT+AGG - chr5_1:65283184-65283203 Msa0744480:intron 15.0%
!!! CAATTTTTTATTTGGGAATT+AGG - chr5_1:65281409-65281428 Msa0744480:three_prime_UTR 20.0%
!!! TCAATGAAATTGATAGTTAT+AGG - chr5_1:65281464-65281483 Msa0744480:three_prime_UTR 20.0%
!!! CAATGAAATTGATAGTTATA+GGG - chr5_1:65281465-65281484 Msa0744480:three_prime_UTR 20.0%
!! TGTAATATTGCAGAAAATAT+TGG + chr5_1:65281535-65281554 None:intergenic 20.0%
!!! ATGTATATGAAGTTTGTTTA+TGG + chr5_1:65281597-65281616 None:intergenic 20.0%
!! ATCAAAACATTCATGATTAT+TGG + chr5_1:65281671-65281690 None:intergenic 20.0%
!!! AATAATCATGAATGTTTTGA+TGG - chr5_1:65281670-65281689 Msa0744480:three_prime_UTR 20.0%
!! AAAATAATCCAACACAATAA+AGG + chr5_1:65281772-65281791 None:intergenic 20.0%
!! GCTAATAATAGTTTAATTCT+TGG - chr5_1:65281959-65281978 Msa0744480:CDS 20.0%
!! AATAGTTTAATTCTTGGTTT+AGG - chr5_1:65281965-65281984 Msa0744480:intron 20.0%
!! TATGAAAATCAGATTTCAAT+AGG - chr5_1:65282508-65282527 Msa0744480:intron 20.0%
!! TGCAAAAAGAAAAACAAAAA+AGG + chr5_1:65282743-65282762 None:intergenic 20.0%
!! TAGTAAAAACAAGTCATTAA+TGG + chr5_1:65282772-65282791 None:intergenic 20.0%
!! CTCAAAGTAATATTAGATAT+AGG + chr5_1:65283153-65283172 None:intergenic 20.0%
!! ATAAAATAACAAACTTCATC+AGG + chr5_1:65283365-65283384 None:intergenic 20.0%
!! TTTTCAATGAAGTGAATCAA+AGG + chr5_1:65281080-65281099 None:intergenic 25.0%
! GAATAAAAATACCTCTATGA+GGG + chr5_1:65281375-65281394 None:intergenic 25.0%
! AGAATAAAAATACCTCTATG+AGG + chr5_1:65281376-65281395 None:intergenic 25.0%
!! TAATGAACTTTGTTGTCTTA+GGG - chr5_1:65281641-65281660 Msa0744480:three_prime_UTR 25.0%
!! GATGGTTGAAATATAACTTT+TGG - chr5_1:65281688-65281707 Msa0744480:three_prime_UTR 25.0%
!!! ATTATTTTTCGCTTCTATGT+TGG - chr5_1:65281784-65281803 Msa0744480:three_prime_UTR 25.0%
! TGTTCTAATTAAACTCTGTT+TGG - chr5_1:65281841-65281860 Msa0744480:CDS 25.0%
!! GTTAGAAAGTTTCTAGATAA+TGG - chr5_1:65281879-65281898 Msa0744480:CDS 25.0%
! GTTTATTCACTATGTCAATT+AGG - chr5_1:65281999-65282018 Msa0744480:intron 25.0%
!!! ATTTTAAGACACAATTTGTC+AGG - chr5_1:65282249-65282268 Msa0744480:intron 25.0%
! TCTCATATATCATTCTTACT+TGG - chr5_1:65282383-65282402 Msa0744480:intron 25.0%
! TTCCTTTCTTCTTGATATTT+AGG - chr5_1:65282420-65282439 Msa0744480:intron 25.0%
!!! AGGCAATTGGAATTTTTTAA+AGG - chr5_1:65282448-65282467 Msa0744480:intron 25.0%
!!! GGCAATTGGAATTTTTTAAA+GGG - chr5_1:65282449-65282468 Msa0744480:intron 25.0%
!!! AATTGGAATTTTTTAAAGGG+AGG - chr5_1:65282452-65282471 Msa0744480:intron 25.0%
! TAATCAGCAGTAACATTATT+TGG + chr5_1:65282490-65282509 None:intergenic 25.0%
! GAAATCATATAGGATGAAAA+AGG + chr5_1:65282801-65282820 None:intergenic 25.0%
! AAACACATACACATTAGTTA+TGG - chr5_1:65282973-65282992 Msa0744480:intron 25.0%
! GGTATTCAAACACATTTATT+TGG - chr5_1:65282994-65283013 Msa0744480:intron 25.0%
! AGAAAGGAACAAAGATATAT+GGG + chr5_1:65283266-65283285 None:intergenic 25.0%
! AAGAAAGGAACAAAGATATA+TGG + chr5_1:65283267-65283286 None:intergenic 25.0%
! TGTATTATTTCGTCAAAGAA+AGG + chr5_1:65283282-65283301 None:intergenic 25.0%
!! TTTTCATCTATCATTTTGGA+CGG + chr5_1:65283428-65283447 None:intergenic 25.0%
!! GCAATTTTCATCTATCATTT+TGG + chr5_1:65283432-65283451 None:intergenic 25.0%
! ATATGAAGTTTGTTTATGGC+TGG + chr5_1:65281593-65281612 None:intergenic 30.0%
! GTAATGAACTTTGTTGTCTT+AGG - chr5_1:65281640-65281659 Msa0744480:three_prime_UTR 30.0%
TACCATATCCTTTATTGTGT+TGG - chr5_1:65281761-65281780 Msa0744480:three_prime_UTR 30.0%
ATCCAACACAATAAAGGATA+TGG + chr5_1:65281766-65281785 None:intergenic 30.0%
TGCCTAAATATCAAGAAGAA+AGG + chr5_1:65282425-65282444 None:intergenic 30.0%
ACAAAGCATATAAGTTACCA+TGG + chr5_1:65282884-65282903 None:intergenic 30.0%
CTTCCTTCTTATCTCATAAA+TGG - chr5_1:65282905-65282924 Msa0744480:intron 30.0%
CATCCATTTATGAGATAAGA+AGG + chr5_1:65282911-65282930 None:intergenic 30.0%
!! TTTTTGTCTAGAACTCTTCT+GGG - chr5_1:65283103-65283122 Msa0744480:intron 30.0%
CAAAACATATATAGGATTCC+TGG - chr5_1:65283192-65283211 Msa0744480:intron 30.0%
CTTTGACGAAATAATACATC+AGG - chr5_1:65283284-65283303 Msa0744480:CDS 30.0%
TAATACATCAGGTGACTTAT+TGG - chr5_1:65283295-65283314 Msa0744480:CDS 30.0%
! TTTCATCTATCATTTTGGAC+GGG + chr5_1:65283427-65283446 None:intergenic 30.0%
ACTAACATAACGGTCATTAT+TGG + chr5_1:65283512-65283531 None:intergenic 30.0%
AAAGCGTGAAACTAACATAA+CGG + chr5_1:65283522-65283541 None:intergenic 30.0%
ATGAAGTGAATCAAAGGAGA+GGG + chr5_1:65281074-65281093 None:intergenic 35.0%
AATGAAGTGAATCAAAGGAG+AGG + chr5_1:65281075-65281094 None:intergenic 35.0%
TAATTCTTGGTTTAGGCAGA+TGG - chr5_1:65281972-65281991 Msa0744480:intron 35.0%
AGACAACAAACTTCAAACCA+GGG + chr5_1:65282027-65282046 None:intergenic 35.0%
TAGACAACAAACTTCAAACC+AGG + chr5_1:65282028-65282047 None:intergenic 35.0%
AGTCACTTCGTCATTGTAAA+TGG + chr5_1:65282147-65282166 None:intergenic 35.0%
TGAGCTCTCTAAATTATGTG+TGG + chr5_1:65282222-65282241 None:intergenic 35.0%
TTGCTCATCCAAATGCATAT+TGG - chr5_1:65282276-65282295 Msa0744480:intron 35.0%
TAACCAGTCCAATATGCATT+TGG + chr5_1:65282287-65282306 None:intergenic 35.0%
!!! GGACTGGTTATTTTACAAGT+AGG - chr5_1:65282297-65282316 Msa0744480:intron 35.0%
TCATGTAACCCTATAAAGCT+TGG + chr5_1:65282323-65282342 None:intergenic 35.0%
GGTTACATGAGAATAAAGAG+TGG - chr5_1:65282333-65282352 Msa0744480:intron 35.0%
TTCTTGATATTTAGGCAGCA+AGG - chr5_1:65282428-65282447 Msa0744480:intron 35.0%
TTTAAAGGGAGGAATGAATC+AGG - chr5_1:65282463-65282482 Msa0744480:intron 35.0%
GATTTCAATAGGCTATGATG+AGG - chr5_1:65282519-65282538 Msa0744480:intron 35.0%
!! TCCACGATTTCTTAATTTGC+AGG - chr5_1:65282598-65282617 Msa0744480:intron 35.0%
!! TTTCTTAATTTGCAGGCAGA+AGG - chr5_1:65282605-65282624 Msa0744480:intron 35.0%
TGGTTTACCAATAAAGCAAG+TGG + chr5_1:65282644-65282663 None:intergenic 35.0%
CATGTGATAAACACAGTAAC+AGG - chr5_1:65282677-65282696 Msa0744480:intron 35.0%
TTATTGGAACTTACTGACCA+TGG - chr5_1:65282864-65282883 Msa0744480:intron 35.0%
CTCATAAATGGATGCACTAA+TGG - chr5_1:65282917-65282936 Msa0744480:intron 35.0%
!! GTTTTTGTCTAGAACTCTTC+TGG - chr5_1:65283102-65283121 Msa0744480:intron 35.0%
TGAACTTCTGATGCAATTGT+AGG - chr5_1:65283486-65283505 Msa0744480:CDS 35.0%
TTATGGCTGGTGATAGCTAT+TGG + chr5_1:65281580-65281599 None:intergenic 40.0%
ATCCTGAGAGAATACCAGTA+TGG + chr5_1:65282121-65282140 None:intergenic 40.0%
ATACTGGTATTCTCTCAGGA+TGG - chr5_1:65282120-65282139 Msa0744480:CDS 40.0%
CATCCAAATGCATATTGGAC+TGG - chr5_1:65282281-65282300 Msa0744480:intron 40.0%
! ACAAGTAGGCCAAGCTTTAT+AGG - chr5_1:65282311-65282330 Msa0744480:intron 40.0%
! CAAGTAGGCCAAGCTTTATA+GGG - chr5_1:65282312-65282331 Msa0744480:intron 40.0%
TATTTAGGCAGCAAGGCAAT+TGG - chr5_1:65282435-65282454 Msa0744480:intron 40.0%
GCCTGCAAATTAAGAAATCG+TGG + chr5_1:65282602-65282621 None:intergenic 40.0%
TGCATCTCCACTTGCTTTAT+TGG - chr5_1:65282634-65282653 Msa0744480:intron 40.0%
TCACATGCGAACTTGATCTT+TGG + chr5_1:65282664-65282683 None:intergenic 40.0%
CTAGACAAAAACCTTTGCAG+TGG + chr5_1:65283094-65283113 None:intergenic 40.0%
ATATAGGATTCCTGGTGAAG+TGG - chr5_1:65283200-65283219 Msa0744480:intron 40.0%
ACCTCTCTTTCTCACTCTCA+CGG - chr5_1:65281180-65281199 Msa0744480:three_prime_UTR 45.0%
!! ACAGAAGTTCTGGAGAGTGA+CGG + chr5_1:65281221-65281240 None:intergenic 45.0%
!! GAGTGTTGGAACAGAAGTTC+TGG + chr5_1:65281231-65281250 None:intergenic 45.0%
ACAACGAAGAGAGTGACAGA+GGG + chr5_1:65281261-65281280 None:intergenic 45.0%
GAATGGAACGATGAGAGTGA+CGG + chr5_1:65281341-65281360 None:intergenic 45.0%
ACTATGTCAATTAGGAGCCC+TGG - chr5_1:65282007-65282026 Msa0744480:intron 45.0%
ACTCACTGTTCATGCCATAC+TGG - chr5_1:65282104-65282123 Msa0744480:CDS 45.0%
TGCCATACTGGTATTCTCTC+AGG - chr5_1:65282116-65282135 Msa0744480:CDS 45.0%
AATAGGAAGTGAGAGATGCG+AGG + chr5_1:65282170-65282189 None:intergenic 45.0%
!! AGCTTTGAAGTGAAGGGGAA+GGG + chr5_1:65282198-65282217 None:intergenic 45.0%
!! TGTGGCAGCTTTGAAGTGAA+GGG + chr5_1:65282204-65282223 None:intergenic 45.0%
! TAATTTGCAGGCAGAAGGCT+TGG - chr5_1:65282610-65282629 Msa0744480:intron 45.0%
TATGACTAGAGTGCACCTGT+TGG + chr5_1:65282706-65282725 None:intergenic 45.0%
CTTCAGAACATACTTGCCAC+AGG - chr5_1:65283065-65283084 Msa0744480:intron 45.0%
! TGTCTAGAACTCTTCTGGGA+TGG - chr5_1:65283107-65283126 Msa0744480:intron 45.0%
AAGAAGTTAGCCACTTCACC+AGG + chr5_1:65283213-65283232 None:intergenic 45.0%
GGTGAAGTGGCTAACTTCTT+TGG - chr5_1:65283213-65283232 Msa0744480:intron 45.0%
!! GAGTGAGAAGGTTCTGGAGA+TGG + chr5_1:65281138-65281157 None:intergenic 50.0%
GAGAGAGAGTGAGAAGGTTC+TGG + chr5_1:65281144-65281163 None:intergenic 50.0%
GAGTGAGAAAGAGAGGTTCC+TGG + chr5_1:65281177-65281196 None:intergenic 50.0%
GACAACGAAGAGAGTGACAG+AGG + chr5_1:65281262-65281281 None:intergenic 50.0%
GCAACGGCTACAGTAACAGA+GGG + chr5_1:65281288-65281307 None:intergenic 50.0%
TGCAACGGCTACAGTAACAG+AGG + chr5_1:65281289-65281308 None:intergenic 50.0%
! AGTGATGGAGCGGTTTGCAA+CGG + chr5_1:65281304-65281323 None:intergenic 50.0%
! AGAGAGTGAGAGTGATGGAG+CGG + chr5_1:65281314-65281333 None:intergenic 50.0%
! TGAGGGAGACAGTGATGGAA+TGG + chr5_1:65281358-65281377 None:intergenic 50.0%
CTCTATGAGGGAGACAGTGA+TGG + chr5_1:65281363-65281382 None:intergenic 50.0%
CATCACTGTCTCCCTCATAG+AGG - chr5_1:65281361-65281380 Msa0744480:three_prime_UTR 50.0%
!! CAGCTTTGAAGTGAAGGGGA+AGG + chr5_1:65282199-65282218 None:intergenic 50.0%
!! GTGGCAGCTTTGAAGTGAAG+GGG + chr5_1:65282203-65282222 None:intergenic 50.0%
!! GTGTGGCAGCTTTGAAGTGA+AGG + chr5_1:65282205-65282224 None:intergenic 50.0%
CACAGTAACAGGCTTCCAAC+AGG - chr5_1:65282688-65282707 Msa0744480:intron 50.0%
TAGCGCACTCATTGGAGGAA+GGG + chr5_1:65282944-65282963 None:intergenic 50.0%
TTAGCGCACTCATTGGAGGA+AGG + chr5_1:65282945-65282964 None:intergenic 50.0%
TCCTCCAATGAGTGCGCTAA+TGG - chr5_1:65282945-65282964 Msa0744480:intron 50.0%
TCCATTAGCGCACTCATTGG+AGG + chr5_1:65282949-65282968 None:intergenic 50.0%
GTCTCCATTAGCGCACTCAT+TGG + chr5_1:65282952-65282971 None:intergenic 50.0%
ACCTTTGCAGTGGAAACCTG+TGG + chr5_1:65283084-65283103 None:intergenic 50.0%
GTGAGGGAGAGAGAGTGAGA+AGG + chr5_1:65281150-65281169 None:intergenic 55.0%
AGAGGTTCCTGGAGCTGTGA+GGG + chr5_1:65281166-65281185 None:intergenic 55.0%
GCCGTGAGAGTGAGAAAGAG+AGG + chr5_1:65281184-65281203 None:intergenic 55.0%
!! GAAGTTCTGGAGAGTGACGG+CGG + chr5_1:65281218-65281237 None:intergenic 55.0%
CAGAGGGAGAGAGTGAGTGT+TGG + chr5_1:65281245-65281264 None:intergenic 55.0%
GAGGGAGAGAGTGAGAGTGA+TGG + chr5_1:65281319-65281338 None:intergenic 55.0%
! GGAACGATGAGAGTGACGGA+GGG + chr5_1:65281337-65281356 None:intergenic 55.0%
TGGAACGATGAGAGTGACGG+AGG + chr5_1:65281338-65281357 None:intergenic 55.0%
GAAGGGGAAGGGACAGGAAT+AGG + chr5_1:65282187-65282206 None:intergenic 55.0%
TGAAGTGAAGGGGAAGGGAC+AGG + chr5_1:65282193-65282212 None:intergenic 55.0%
GCCACAGGTTTCCACTGCAA+AGG - chr5_1:65283080-65283099 Msa0744480:intron 55.0%
TCTCTCTCCCTCACAGCTCC+AGG - chr5_1:65281156-65281175 Msa0744480:three_prime_UTR 60.0%
GAGAGGTTCCTGGAGCTGTG+AGG + chr5_1:65281167-65281186 None:intergenic 60.0%
AGTGACGGCGGCGAGTGAGA+CGG + chr5_1:65281206-65281225 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 65281053 65283550 65281053 ID=Msa0744480;Name=Msa0744480
chr5_1 mRNA 65281053 65283550 65281053 ID=Msa0744480-mRNA-1;Parent=Msa0744480;Name=Msa0744480-mRNA-1;_AED=0.11;_eAED=0.11;_QI=0|0.66|0.75|1|0.33|0.25|4|683|194
chr5_1 exon 65281053 65281516 65281053 ID=Msa0744480-mRNA-1:exon:19379;Parent=Msa0744480-mRNA-1
chr5_1 exon 65281609 65281983 65281609 ID=Msa0744480-mRNA-1:exon:19378;Parent=Msa0744480-mRNA-1
chr5_1 exon 65282063 65282161 65282063 ID=Msa0744480-mRNA-1:exon:19377;Parent=Msa0744480-mRNA-1
chr5_1 exon 65283221 65283550 65283221 ID=Msa0744480-mRNA-1:exon:19376;Parent=Msa0744480-mRNA-1
chr5_1 CDS 65283221 65283550 65283221 ID=Msa0744480-mRNA-1:cds;Parent=Msa0744480-mRNA-1
chr5_1 CDS 65282063 65282161 65282063 ID=Msa0744480-mRNA-1:cds;Parent=Msa0744480-mRNA-1
chr5_1 CDS 65281828 65281983 65281828 ID=Msa0744480-mRNA-1:cds;Parent=Msa0744480-mRNA-1
chr5_1 three_prime_UTR 65281609 65281827 65281609 ID=Msa0744480-mRNA-1:three_prime_utr;Parent=Msa0744480-mRNA-1
chr5_1 three_prime_UTR 65281053 65281516 65281053 ID=Msa0744480-mRNA-1:three_prime_utr;Parent=Msa0744480-mRNA-1
Gene Sequence

>Msa0744480

GCACTTTTATTTTCGTTTCCCTCTCCTTTGATTCACTTCATTGAAAACAAATCAAAATCTCTTTCTCTCATTATCTCTTTCTCCATCTCCAGAACCTTCTCACTCTCTCTCCCTCACAGCTCCAGGAACCTCTCTTTCTCACTCTCACGGCCGTCTCACTCGCCGCCGTCACTCTCCAGAACTTCTGTTCCAACACTCACTCTCTCCCTCTGTCACTCTCTTCGTTGTCAAACCCTCTGTTACTGTAGCCGTTGCAAACCGCTCCATCACTCTCACTCTCTCCCTCCGTCACTCTCATCGTTCCATTCCATCACTGTCTCCCTCATAGAGGCAGCAAGGCAATTGGAATTTTTTAAAGGGAGGAATGAATCAGGACCAAATAATGTTACTGCTGATTATGAAAATCAGATTTCAATAGGCTATGATGAGGCAGAAGGCTTGGTTGCATCTCCACTTGCTTTATTGGTAAACCAAAGATCAAGTTCGCATGTGATAAACACAGTAACAGGCTTCCAACAGGTGCACTCTAGTCATAGAATTCATTTATCTCCTTTTTTGTTTTTCTTTTTGCAAGAAATCCATTAA
Protein sequence

>Msa0744480

ALLFSFPSPLIHFIENKSKSLSLIISFSISRTFSLSLPHSSRNLSFSLSRPSHSPPSLSRTSVPTLTLSLCHSLRCQTLCYCSRCKPLHHSHSLPPSLSSFHSITVSLIEAARQLEFFKGRNESGPNNVTADYENQISIGYDEAEGLVASPLALLVNQRSSSHVINTVTGFQQVHSSHRIHLSPFLFFFLQEIH*