AlfalfaGEDB | Msa1095960

M. sativa cultivar zhongmu-4 / Msa1095960

KEGG: -
GO: -
PFAM: PF13963.9

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1095960	XP_024628770.2	84.810	79	12	0	1	79	24	102	3.90e-42	150

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1095960	A0A1S3VFN4	59.494	79	31	1	1	78	1	79	1.24e-24	109

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0744480	Msa1095960	0.817428	3.314392e-52	-8.615850e-47
Msa0845370	Msa1095960	0.822081	2.866207e-53	-8.615850e-47
Msa1021420	Msa1095960	0.846005	2.849207e-59	-8.615850e-47
Msa1095960	Msa1131350	-0.800010	1.760824e-48	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1095960	MtrunA17_Chr7g0252171	55.172	87	5	1	103	155	378	464	5.95e-18	80.5
Msa1095960	MtrunA17_Chr3g0081411	40.260	77	43	2	1	74	1	77	3.18e-16	70.1
Msa1095960	MtrunA17_Chr8g0371111	32.653	98	64	2	1	96	12	109	5.13e-13	63.2
Msa1095960	MtrunA17_Chr2g0302181	40.260	77	43	2	1	74	1	77	2.99e-12	60.8
Msa1095960	MtrunA17_Chr8g0393091	36.364	77	44	2	1	74	1	75	7.94e-11	60.1

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 60 sgRNAs with CRISPR-Local

Find 272 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACACATACACATTAGTTA+TGG	0.511165	7_3:+19563556	Msa1095960:three_prime_UTR
AAATATGTCAAAACATATAT+AGG	0.355946	7_3:+19563767	Msa1095960:three_prime_UTR
AAGAAAGGAACAAAGATATA+TGG	0.298600	7_3:-19563847	None:intergenic
AAGAAGTTAGCCACTTCACC+AGG	0.558942	7_3:-19563793	None:intergenic
AAGACCTAGGAACTATGGAT+CGG	0.635897	7_3:+19559634	None:intergenic
AAGGGAGCGGCAAATACACA+TGG	0.696420	7_3:-19560855	None:intergenic
AATCCCATGGATAGAAATAA+AGG	0.380452	7_3:+19560907	Msa1095960:CDS
AATGAATCAGGAATTTCAAT+AGG	0.440417	7_3:+19563091	Msa1095960:CDS
AATTTCAATAGGCTATGATG+AGG	0.681055	7_3:+19563102	Msa1095960:CDS
ACAAAGCATATAAGTTAACA+TGG	0.643457	7_3:-19563464	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CATAACAACATTCTAAGCAT+GGG	-	chr7_3:19559764-19559783	None:intergenic	30.0%
CAAGATCACTTAGTATGTAA+TGG	+	chr7_3:19559807-19559826	Msa1095960:CDS	30.0%
TTGATGAAAGCTATACATGT+TGG	+	chr7_3:19559832-19559851	Msa1095960:CDS	30.0%
TCAAAGATACCATTGTATGA+TGG	+	chr7_3:19560014-19560033	Msa1095960:intron	30.0%
TAACATTTGTTTAGCCTCAT+AGG	-	chr7_3:19560183-19560202	None:intergenic	30.0%
ATAATATATCATCTCAGCTC+CGG	-	chr7_3:19560688-19560707	None:intergenic	30.0%
TATTATATGGATTCATTGCC+GGG	+	chr7_3:19560702-19560721	Msa1095960:intron	30.0%
AATCCCATGGATAGAAATAA+AGG	+	chr7_3:19560907-19560926	Msa1095960:CDS	30.0%
TAAATAGTAATTGGTTGTGC+TGG	+	chr7_3:19561376-19561395	Msa1095960:intron	30.0%
TATACCATCCTTTATTGTGT+TGG	+	chr7_3:19562332-19562351	Msa1095960:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7_3	gene	19559648	19564088	19559648	ID=Msa1095960;Name=Msa1095960
chr7_3	mRNA	19559648	19564088	19559648	ID=Msa1095960-mRNA-1;Parent=Msa1095960;Name=Msa1095960-mRNA-1;_AED=0.37;_eAED=0.37;_QI=0\|0.5\|0.6\|0.8\|0.25\|0.4\|5\|638\|176
chr7_3	exon	19559648	19559880	19559648	ID=Msa1095960-mRNA-1:exon:4230;Parent=Msa1095960-mRNA-1
chr7_3	exon	19560853	19560928	19560853	ID=Msa1095960-mRNA-1:exon:4231;Parent=Msa1095960-mRNA-1
chr7_3	exon	19563058	19563123	19563058	ID=Msa1095960-mRNA-1:exon:4232;Parent=Msa1095960-mRNA-1
chr7_3	exon	19563203	19563577	19563203	ID=Msa1095960-mRNA-1:exon:4233;Parent=Msa1095960-mRNA-1
chr7_3	exon	19563670	19564088	19563670	ID=Msa1095960-mRNA-1:exon:4234;Parent=Msa1095960-mRNA-1
chr7_3	CDS	19559648	19559880	19559648	ID=Msa1095960-mRNA-1:cds;Parent=Msa1095960-mRNA-1
chr7_3	CDS	19560853	19560928	19560853	ID=Msa1095960-mRNA-1:cds;Parent=Msa1095960-mRNA-1
chr7_3	CDS	19563058	19563123	19563058	ID=Msa1095960-mRNA-1:cds;Parent=Msa1095960-mRNA-1
chr7_3	CDS	19563203	19563358	19563203	ID=Msa1095960-mRNA-1:cds;Parent=Msa1095960-mRNA-1
chr7_3	three_prime_UTR	19563359	19563577	19563359	ID=Msa1095960-mRNA-1:three_prime_utr;Parent=Msa1095960-mRNA-1
chr7_3	three_prime_UTR	19563670	19564088	19563670	ID=Msa1095960-mRNA-1:three_prime_utr;Parent=Msa1095960-mRNA-1

Gene Sequence

>Msa1095960

ATGGATCGGAAATGGGTGTCTGCGAATCGAGCGTCAAAGGAGTACAGAGAAGGAGTGAAAGAGTTTGTTGAGTTTGTTGTTGCTCATGCAAAAGACAGCAATAACATCATATGCCCATGCTTAGAATGTTGTTATGGAGCAGTTAGTGCGGAGCATTTGCAAGATCACTTAGTATGTAATGGAATTGATGAAAGCTATACATGTTGGACAATGCACGGGGAAGAAGAAACTGATGCCATGTGTATTTGCCGCTCCCTTCATCCCAAGTCAAAATCAAAGTCAAGTATAATCCCATGGATAGAAATAAAGGCAGCAAGGCAATTGGAATTTTTTAAAGGGAGGAATGAATCAGGAATTTCAATAGGCTATGATGAGGCAGAAGGCTTGGTTGCATCTCCACTTGCTTTATTGGTAAACCAAAGATCAAGTTCGCATGTGATAAACACAGTAACAGGCTTCCAACAGGTGCACTCTAGTCATAGAATTCATTTATCTCCTTTTTTGTTTTTCTTTTTGCAAGAAATCCATTAA

Protein sequence

>Msa1095960

MDRKWVSANRASKEYREGVKEFVEFVVAHAKDSNNIICPCLECCYGAVSAEHLQDHLVCNGIDESYTCWTMHGEEETDAMCICRSLHPKSKSKSSIIPWIEIKAARQLEFFKGRNESGISIGYDEAEGLVASPLALLVNQRSSSHVINTVTGFQQVHSSHRIHLSPFLFFFLQEIH*