AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1095960

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1095960 MtrunA17_Chr7g0252171 55.172 87 5 1 103 155 378 464 5.95e-18 80.5
Msa1095960 MtrunA17_Chr3g0081411 40.260 77 43 2 1 74 1 77 3.18e-16 70.1
Msa1095960 MtrunA17_Chr8g0371111 32.653 98 64 2 1 96 12 109 5.13e-13 63.2
Msa1095960 MtrunA17_Chr2g0302181 40.260 77 43 2 1 74 1 77 2.99e-12 60.8
Msa1095960 MtrunA17_Chr8g0393091 36.364 77 44 2 1 74 1 75 7.94e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 60 sgRNAs with CRISPR-Local

Find 272 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCATCTCCACTTGCTTTAT+TGG 0.176059 7_3:+19563217 Msa1095960:CDS
CTTCCTTCTTATCTCATAAA+TGG 0.193129 7_3:+19563488 Msa1095960:three_prime_UTR
TAATACATCAGGTGACTTAT+TGG 0.198252 7_3:+19563878 Msa1095960:three_prime_UTR
TATAATTTGATAGATATTAT+TGG 0.208885 7_3:+19563431 Msa1095960:three_prime_UTR
GGTGAAGTGGCTAACTTCTT+TGG 0.252456 7_3:+19563796 Msa1095960:three_prime_UTR
CTCATAAATGGATGCACTAA+TGG 0.290935 7_3:+19563500 Msa1095960:three_prime_UTR
AAGAAAGGAACAAAGATATA+TGG 0.298600 7_3:-19563847 None:intergenic
GAAATCATATAGGATGAAAA+AGG 0.331410 7_3:-19563381 None:intergenic
TCACATGCGAACTTGATCTT+TGG 0.334210 7_3:-19563244 None:intergenic
TCCTCCAATGAGTGCGCTAA+TGG 0.353003 7_3:+19563528 Msa1095960:three_prime_UTR
AAATATGTCAAAACATATAT+AGG 0.355946 7_3:+19563767 Msa1095960:three_prime_UTR
TAGGAACTATGGATCGGAAA+TGG 0.372343 7_3:+19559640 None:intergenic
AATCCCATGGATAGAAATAA+AGG 0.380452 7_3:+19560907 Msa1095960:CDS
CAAAACATATATAGGATTCC+TGG 0.384126 7_3:+19563775 Msa1095960:three_prime_UTR
TTTAAAGGGAGGAATGAATC+AGG 0.398018 7_3:+19563079 Msa1095960:CDS
CATGTGATAAACACAGTAAC+AGG 0.403502 7_3:+19563260 Msa1095960:CDS
CAAGATCACTTAGTATGTAA+TGG 0.408379 7_3:+19559807 Msa1095960:CDS
ATAAAATAACAAACTTCATC+AGG 0.411509 7_3:-19563945 None:intergenic
TATACCTTTATTTCTATCCA+TGG 0.411676 7_3:-19560911 None:intergenic
TGAACTTCTGATGCAATTGT+AGG 0.413663 7_3:+19564069 Msa1095960:three_prime_UTR
CTTTGACGAAATAATACATC+AGG 0.421986 7_3:+19563867 Msa1095960:three_prime_UTR
TATTTAGGCAGCAAGGCAAT+TGG 0.434701 7_3:+19563051 Msa1095960:intron
AATGAATCAGGAATTTCAAT+AGG 0.440417 7_3:+19563091 Msa1095960:CDS
AGAAAGGAACAAAGATATAT+GGG 0.447690 7_3:-19563846 None:intergenic
TGTATTATTTCGTCAAAGAA+AGG 0.463318 7_3:-19563862 None:intergenic
GTCTCCATTAGCGCACTCAT+TGG 0.465837 7_3:-19563532 None:intergenic
TAATTTGCAGGCAGAAGGCT+TGG 0.477127 7_3:+19563193 Msa1095960:intron
CACAGTAACAGGCTTCCAAC+AGG 0.481101 7_3:+19563271 Msa1095960:CDS
CTCAAAGTAATATTAGATAT+AGG 0.489671 7_3:-19563733 None:intergenic
CATCCATTTATGAGATAAGA+AGG 0.498458 7_3:-19563491 None:intergenic
AGGAACTATGGATCGGAAAT+GGG 0.501221 7_3:+19559641 None:intergenic
CCATGCTTAGAATGTTGTTA+TGG 0.504100 7_3:+19559762 Msa1095960:CDS
ATACCTTTATTTCTATCCAT+GGG 0.510170 7_3:-19560910 None:intergenic
AAACACATACACATTAGTTA+TGG 0.511165 7_3:+19563556 Msa1095960:three_prime_UTR
TCCATTAGCGCACTCATTGG+AGG 0.514548 7_3:-19563529 None:intergenic
TTTCTTAATTTGCAGGCAGA+AGG 0.528909 7_3:+19563188 Msa1095960:intron
TGTCTAGAACTCTTCTGGGA+TGG 0.529191 7_3:+19563690 Msa1095960:three_prime_UTR
GTCTGCGAATCGAGCGTCAA+AGG 0.529635 7_3:+19559665 Msa1095960:CDS
CCATAACAACATTCTAAGCA+TGG 0.543228 7_3:-19559762 None:intergenic
GCCACAGGTTTCCACTGCAA+AGG 0.543513 7_3:+19563663 Msa1095960:intron
TTCTTGATATTTAGGCAGCA+AGG 0.550166 7_3:+19563044 Msa1095960:intron
AAGAAGTTAGCCACTTCACC+AGG 0.558942 7_3:-19563793 None:intergenic
TTGACTTGGGATGAAGGGAG+CGG 0.565101 7_3:-19560868 None:intergenic
TTGATGAAAGCTATACATGT+TGG 0.567051 7_3:+19559832 Msa1095960:CDS
ATATAGGATTCCTGGTGAAG+TGG 0.572171 7_3:+19563783 Msa1095960:three_prime_UTR
TGGTTTACCAATAAAGCAAG+TGG 0.574147 7_3:-19563224 None:intergenic
ATACATGTTGGACAATGCAC+GGG 0.577005 7_3:+19559844 Msa1095960:CDS
TATACATGTTGGACAATGCA+CGG 0.583990 7_3:+19559843 Msa1095960:CDS
CATAACAACATTCTAAGCAT+GGG 0.591005 7_3:-19559761 None:intergenic
TTAGCGCACTCATTGGAGGA+AGG 0.595443 7_3:-19563525 None:intergenic
CAAAGTCAAGTATAATCCCA+TGG 0.596399 7_3:+19560894 Msa1095960:CDS
TAGCGCACTCATTGGAGGAA+GGG 0.596922 7_3:-19563524 None:intergenic
TATGACTAGAGTGCACCTGT+TGG 0.613837 7_3:-19563286 None:intergenic
GCGTCAAAGGAGTACAGAGA+AGG 0.614443 7_3:+19559678 Msa1095960:CDS
AAGACCTAGGAACTATGGAT+CGG 0.635897 7_3:+19559634 None:intergenic
ACAAAGCATATAAGTTAACA+TGG 0.643457 7_3:-19563464 None:intergenic
TTGTTATGGAGCAGTTAGTG+CGG 0.671711 7_3:+19559776 Msa1095960:CDS
AATTTCAATAGGCTATGATG+AGG 0.681055 7_3:+19563102 Msa1095960:CDS
AAGGGAGCGGCAAATACACA+TGG 0.696420 7_3:-19560855 None:intergenic
TACATGTTGGACAATGCACG+GGG 0.721718 7_3:+19559845 Msa1095960:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCATAAATCAATTTTTTATT+TGG + chr7_3:19561974-19561993 Msa1095960:intron 10.0%
!!! CATAAATCAATTTTTTATTT+GGG + chr7_3:19561975-19561994 Msa1095960:intron 10.0%
!! AAAAAAATAAGAAATCATAT+AGG - chr7_3:19563394-19563413 None:intergenic 10.0%
!! TATAATTTGATAGATATTAT+TGG + chr7_3:19563431-19563450 Msa1095960:three_prime_UTR 10.0%
!!! TATGCATCATTTTATTTTAT+TGG + chr7_3:19560272-19560291 Msa1095960:intron 15.0%
!! GAAATATATGTAAATTATGA+TGG + chr7_3:19560624-19560643 Msa1095960:intron 15.0%
!! ATTTATGAAAGAGATAATAT+TGG + chr7_3:19561075-19561094 Msa1095960:intron 15.0%
!!! TATTTATATTTGAAGTACTT+TGG + chr7_3:19561213-19561232 Msa1095960:intron 15.0%
!!! GAGTTATTTTAAATAGTAAT+TGG + chr7_3:19561367-19561386 Msa1095960:intron 15.0%
!!! AATTTTTTATTTGGGAATTA+GGG + chr7_3:19561983-19562002 Msa1095960:intron 15.0%
!! TTGATGAAAAATCAATATTT+AGG + chr7_3:19562077-19562096 Msa1095960:intron 15.0%
!! TGATGAAAAATCAATATTTA+GGG + chr7_3:19562078-19562097 Msa1095960:intron 15.0%
!!! TTACAATTTAAGACAATTTA+AGG + chr7_3:19562123-19562142 Msa1095960:intron 15.0%
!! AACAATAGACATAATAATAT+TGG - chr7_3:19562396-19562415 None:intergenic 15.0%
!! AAATATGTCAAAACATATAT+AGG + chr7_3:19563767-19563786 Msa1095960:three_prime_UTR 15.0%
!! TATTAAACTTATGTGTCAAT+AGG - chr7_3:19560505-19560524 None:intergenic 20.0%
!!! AAGGTATATTTTCTAATCAA+TGG + chr7_3:19560926-19560945 Msa1095960:intron 20.0%
!!! AAATCACATACATACATTTT+CGG - chr7_3:19561119-19561138 None:intergenic 20.0%
!! GTAATCATATATATATAGCT+AGG + chr7_3:19561157-19561176 Msa1095960:intron 20.0%
!!! AAAAATTGCCTTAATTTTGT+GGG - chr7_3:19561568-19561587 None:intergenic 20.0%
!!! CAATTTTTTATTTGGGAATT+AGG + chr7_3:19561982-19562001 Msa1095960:intron 20.0%
!!! TCAATGAAATTGATAGTTAT+AGG + chr7_3:19562037-19562056 Msa1095960:intron 20.0%
!!! CAATGAAATTGATAGTTATA+GGG + chr7_3:19562038-19562057 Msa1095960:intron 20.0%
!! TGTAATATTGCAGAAAATAT+TGG - chr7_3:19562108-19562127 None:intergenic 20.0%
!!! ATGTATATGAAGTTTGTTTA+TGG - chr7_3:19562170-19562189 None:intergenic 20.0%
!!! TAATGAACTTTGTTGTTTTA+GGG + chr7_3:19562214-19562233 Msa1095960:intron 20.0%
!! ATCAAAACATTCATGATTAT+TGG - chr7_3:19562244-19562263 None:intergenic 20.0%
!!! AATAATCATGAATGTTTTGA+TGG + chr7_3:19562243-19562262 Msa1095960:intron 20.0%
!! AAAATAATCCAACACAATAA+AGG - chr7_3:19562343-19562362 None:intergenic 20.0%
!! GCTAATAATAGTTTAATTCT+TGG + chr7_3:19562530-19562549 Msa1095960:intron 20.0%
!! AATAGTTTAATTCTTGGTTT+AGG + chr7_3:19562536-19562555 Msa1095960:intron 20.0%
!! TGCAAAAAGAAAAACAAAAA+AGG - chr7_3:19563326-19563345 None:intergenic 20.0%
!! TAGTAAAAACAAGTCATTAA+TGG - chr7_3:19563355-19563374 None:intergenic 20.0%
!! CTCAAAGTAATATTAGATAT+AGG - chr7_3:19563736-19563755 None:intergenic 20.0%
!! ATAAAATAACAAACTTCATC+AGG - chr7_3:19563948-19563967 None:intergenic 20.0%
! TAAATTCACAAGATTGTCTA+CGG + chr7_3:19560043-19560062 Msa1095960:intron 25.0%
! AAACAAATGTTACGTTCTAT+TGG + chr7_3:19560191-19560210 Msa1095960:intron 25.0%
! CGAAATAAAATGCAATCTTT+AGG - chr7_3:19560245-19560264 None:intergenic 25.0%
!! TGTTCTGTTATTCCTATTTT+TGG + chr7_3:19560315-19560334 Msa1095960:intron 25.0%
!!! GTTCTGTTATTCCTATTTTT+GGG + chr7_3:19560316-19560335 Msa1095960:intron 25.0%
! AGCTGAGATGATATATTATA+TGG + chr7_3:19560689-19560708 Msa1095960:intron 25.0%
! ATATTATATGGATTCATTGC+CGG + chr7_3:19560701-19560720 Msa1095960:intron 25.0%
!!! ATACCTTTATTTCTATCCAT+GGG - chr7_3:19560913-19560932 None:intergenic 25.0%
!!! TATACCTTTATTTCTATCCA+TGG - chr7_3:19560914-19560933 None:intergenic 25.0%
!!! TTTTTTGACAACGTAATCAA+TGG + chr7_3:19560952-19560971 Msa1095960:intron 25.0%
!!! GGTATTTTTAATTCATCATG+TGG + chr7_3:19560973-19560992 Msa1095960:intron 25.0%
!! AATCACATACATACATTTTC+GGG - chr7_3:19561118-19561137 None:intergenic 25.0%
!!! AACTAGCTTATCTTTAGAAT+AGG - chr7_3:19561256-19561275 None:intergenic 25.0%
! TCACTATTTCAACTAATTTG+CGG - chr7_3:19561491-19561510 None:intergenic 25.0%
!! TAACAAATGCTTTTCAATCT+TGG + chr7_3:19561529-19561548 Msa1095960:intron 25.0%
!! AAAATTGCCTTAATTTTGTG+GGG - chr7_3:19561567-19561586 None:intergenic 25.0%
!! GAAAAATTGCCTTAATTTTG+TGG - chr7_3:19561569-19561588 None:intergenic 25.0%
! GAATAAAAATACCTCTATGA+GGG - chr7_3:19561948-19561967 None:intergenic 25.0%
! AGAATAAAAATACCTCTATG+AGG - chr7_3:19561949-19561968 None:intergenic 25.0%
!!! GTAATGAACTTTGTTGTTTT+AGG + chr7_3:19562213-19562232 Msa1095960:intron 25.0%
!! GATGGTTGAAATATAACTTT+TGG + chr7_3:19562261-19562280 Msa1095960:intron 25.0%
!!! ATTATTTTTCGCTTCTATGT+TGG + chr7_3:19562355-19562374 Msa1095960:intron 25.0%
! TGTTCTAATTAAACTCTGTT+TGG + chr7_3:19562412-19562431 Msa1095960:intron 25.0%
!! GTTAGAAAGTTTCTAGATAA+TGG + chr7_3:19562450-19562469 Msa1095960:intron 25.0%
! GTTTATTCACTATGTCAATT+AGG + chr7_3:19562570-19562589 Msa1095960:intron 25.0%
!!! ATTTTAAGACACAATTTGTC+AGG + chr7_3:19562865-19562884 Msa1095960:intron 25.0%
! TCTCATATATCATTCTTACT+TGG + chr7_3:19562999-19563018 Msa1095960:intron 25.0%
! TTCCTTTCTTCTTGATATTT+AGG + chr7_3:19563036-19563055 Msa1095960:intron 25.0%
!!! AGGCAATTGGAATTTTTTAA+AGG + chr7_3:19563064-19563083 Msa1095960:CDS 25.0%
!!! GGCAATTGGAATTTTTTAAA+GGG + chr7_3:19563065-19563084 Msa1095960:CDS 25.0%
!!! AATTGGAATTTTTTAAAGGG+AGG + chr7_3:19563068-19563087 Msa1095960:CDS 25.0%
! AATGAATCAGGAATTTCAAT+AGG + chr7_3:19563091-19563110 Msa1095960:CDS 25.0%
! GAAATCATATAGGATGAAAA+AGG - chr7_3:19563384-19563403 None:intergenic 25.0%
! ACAAAGCATATAAGTTAACA+TGG - chr7_3:19563467-19563486 None:intergenic 25.0%
! AAACACATACACATTAGTTA+TGG + chr7_3:19563556-19563575 Msa1095960:three_prime_UTR 25.0%
! GGTATTCAAACACATTTATT+TGG + chr7_3:19563577-19563596 Msa1095960:intron 25.0%
! AGAAAGGAACAAAGATATAT+GGG - chr7_3:19563849-19563868 None:intergenic 25.0%
! AAGAAAGGAACAAAGATATA+TGG - chr7_3:19563850-19563869 None:intergenic 25.0%
! TGTATTATTTCGTCAAAGAA+AGG - chr7_3:19563865-19563884 None:intergenic 25.0%
!! TTTTCATCTATCATTTTGGA+CGG - chr7_3:19564011-19564030 None:intergenic 25.0%
!! GCAATTTTCATCTATCATTT+TGG - chr7_3:19564015-19564034 None:intergenic 25.0%
CATAACAACATTCTAAGCAT+GGG - chr7_3:19559764-19559783 None:intergenic 30.0%
CAAGATCACTTAGTATGTAA+TGG + chr7_3:19559807-19559826 Msa1095960:CDS 30.0%
TTGATGAAAGCTATACATGT+TGG + chr7_3:19559832-19559851 Msa1095960:CDS 30.0%
TCAAAGATACCATTGTATGA+TGG + chr7_3:19560014-19560033 Msa1095960:intron 30.0%
! TATAACTTAAAAGCACGCAA+TGG + chr7_3:19560077-19560096 Msa1095960:intron 30.0%
TAACATTTGTTTAGCCTCAT+AGG - chr7_3:19560183-19560202 None:intergenic 30.0%
!! ATGTAAATTATGATGGATGC+AGG + chr7_3:19560631-19560650 Msa1095960:intron 30.0%
ATAATATATCATCTCAGCTC+CGG - chr7_3:19560688-19560707 None:intergenic 30.0%
TATTATATGGATTCATTGCC+GGG + chr7_3:19560702-19560721 Msa1095960:intron 30.0%
!!! CTTGACTTTGATTTTGACTT+GGG - chr7_3:19560884-19560903 None:intergenic 30.0%
!!! ACTTGACTTTGATTTTGACT+TGG - chr7_3:19560885-19560904 None:intergenic 30.0%
AATCCCATGGATAGAAATAA+AGG + chr7_3:19560907-19560926 Msa1095960:CDS 30.0%
! ATCACATACATACATTTTCG+GGG - chr7_3:19561117-19561136 None:intergenic 30.0%
! TTCTAAAGATAAGCTAGTTG+AGG + chr7_3:19561257-19561276 Msa1095960:intron 30.0%
TAAATAGTAATTGGTTGTGC+TGG + chr7_3:19561376-19561395 Msa1095960:intron 30.0%
! ACTATTTCAACTAATTTGCG+GGG - chr7_3:19561489-19561508 None:intergenic 30.0%
! CACTATTTCAACTAATTTGC+GGG - chr7_3:19561490-19561509 None:intergenic 30.0%
! AGCATTTGTTACAGCAAAAA+GGG - chr7_3:19561520-19561539 None:intergenic 30.0%
! AAGCATTTGTTACAGCAAAA+AGG - chr7_3:19561521-19561540 None:intergenic 30.0%
! AAATGCTTTTCAATCTTGGA+CGG + chr7_3:19561533-19561552 Msa1095960:intron 30.0%
! AAATTGCCTTAATTTTGTGG+GGG - chr7_3:19561566-19561585 None:intergenic 30.0%
! GTTTTCAATGAGTGAATCAA+AGG - chr7_3:19561654-19561673 None:intergenic 30.0%
! ATATGAAGTTTGTTTATGGC+TGG - chr7_3:19562166-19562185 None:intergenic 30.0%
TATACCATCCTTTATTGTGT+TGG + chr7_3:19562332-19562351 Msa1095960:intron 30.0%
TAATCCAACACAATAAAGGA+TGG - chr7_3:19562339-19562358 None:intergenic 30.0%
TGCCTAAATATCAAGAAGAA+AGG - chr7_3:19563041-19563060 None:intergenic 30.0%
AATTTCAATAGGCTATGATG+AGG + chr7_3:19563102-19563121 Msa1095960:CDS 30.0%
CTTCCTTCTTATCTCATAAA+TGG + chr7_3:19563488-19563507 Msa1095960:three_prime_UTR 30.0%
CATCCATTTATGAGATAAGA+AGG - chr7_3:19563494-19563513 None:intergenic 30.0%
!! TTTTTGTCTAGAACTCTTCT+GGG + chr7_3:19563686-19563705 Msa1095960:three_prime_UTR 30.0%
CAAAACATATATAGGATTCC+TGG + chr7_3:19563775-19563794 Msa1095960:three_prime_UTR 30.0%
CTTTGACGAAATAATACATC+AGG + chr7_3:19563867-19563886 Msa1095960:three_prime_UTR 30.0%
TAATACATCAGGTGACTTAT+TGG + chr7_3:19563878-19563897 Msa1095960:three_prime_UTR 30.0%
! TTTCATCTATCATTTTGGAC+GGG - chr7_3:19564010-19564029 None:intergenic 30.0%
CCATAACAACATTCTAAGCA+TGG - chr7_3:19559765-19559784 None:intergenic 35.0%
CCATGCTTAGAATGTTGTTA+TGG + chr7_3:19559762-19559781 Msa1095960:CDS 35.0%
TATACATGTTGGACAATGCA+CGG + chr7_3:19559843-19559862 Msa1095960:CDS 35.0%
ATTGTCCCAAAATGTTTGAG+AGG + chr7_3:19559979-19559998 Msa1095960:intron 35.0%
!! ACTAGCCTCTCAAACATTTT+GGG - chr7_3:19559987-19560006 None:intergenic 35.0%
!! TACTAGCCTCTCAAACATTT+TGG - chr7_3:19559988-19560007 None:intergenic 35.0%
TTAGCACATCCATCATACAA+TGG - chr7_3:19560026-19560045 None:intergenic 35.0%
AAAATTTGATCAAGCCTCGT+GGG + chr7_3:19560405-19560424 Msa1095960:intron 35.0%
TATCTCACGAGGATAAACAA+GGG + chr7_3:19560462-19560481 Msa1095960:intron 35.0%
!! TAAGAAGCTGATTTTTGCAC+CGG + chr7_3:19560523-19560542 Msa1095960:intron 35.0%
!! ATTTTTGCACCGGTCAATTT+AGG + chr7_3:19560533-19560552 Msa1095960:intron 35.0%
!! TTTTTGCACCGGTCAATTTA+GGG + chr7_3:19560534-19560553 Msa1095960:intron 35.0%
AAAACTCACCCTAAATTGAC+CGG - chr7_3:19560545-19560564 None:intergenic 35.0%
TAATATATCATCTCAGCTCC+GGG - chr7_3:19560687-19560706 None:intergenic 35.0%
ATTATATGGATTCATTGCCG+GGG + chr7_3:19560703-19560722 Msa1095960:intron 35.0%
CTTCTTACAACGTCAATGTT+AGG - chr7_3:19560735-19560754 None:intergenic 35.0%
!!! TGATTTTGACTTGGGATGAA+GGG - chr7_3:19560876-19560895 None:intergenic 35.0%
!!! TTGATTTTGACTTGGGATGA+AGG - chr7_3:19560877-19560896 None:intergenic 35.0%
CAAAGTCAAGTATAATCCCA+TGG + chr7_3:19560894-19560913 Msa1095960:CDS 35.0%
!! TCAATGCCATTAGTTTGCAT+AGG - chr7_3:19561034-19561053 None:intergenic 35.0%
ACTAAGATACTCGACCATAA+AGG - chr7_3:19561301-19561320 None:intergenic 35.0%
! CTATTTCAACTAATTTGCGG+GGG - chr7_3:19561488-19561507 None:intergenic 35.0%
! CATTTGTTACAGCAAAAAGG+GGG - chr7_3:19561518-19561537 None:intergenic 35.0%
! GCATTTGTTACAGCAAAAAG+GGG - chr7_3:19561519-19561538 None:intergenic 35.0%
TTCAAACCCCCACAAAATTA+AGG + chr7_3:19561557-19561576 Msa1095960:intron 35.0%
AATGAGTGAATCAAAGGAGA+GGG - chr7_3:19561648-19561667 None:intergenic 35.0%
AGACAACAAACTTCAAACCA+GGG - chr7_3:19562598-19562617 None:intergenic 35.0%
TAGACAACAAACTTCAAACC+AGG - chr7_3:19562599-19562618 None:intergenic 35.0%
! TGTTGATACAATGCATTGAC+TGG - chr7_3:19562718-19562737 None:intergenic 35.0%
TTGTATCAACACCATATACC+AGG + chr7_3:19562727-19562746 Msa1095960:intron 35.0%
TGTATCAACACCATATACCA+GGG + chr7_3:19562728-19562747 Msa1095960:intron 35.0%
GTAAAAATAACTAACTCCCC+TGG - chr7_3:19562748-19562767 None:intergenic 35.0%
TGAGCTCTCTAAATTATGTG+TGG - chr7_3:19562838-19562857 None:intergenic 35.0%
TTGCTCATCCAAATGCATAT+TGG + chr7_3:19562892-19562911 Msa1095960:intron 35.0%
TAACCAGTCCAATATGCATT+TGG - chr7_3:19562903-19562922 None:intergenic 35.0%
!!! GGACTGGTTATTTTACAAGT+AGG + chr7_3:19562913-19562932 Msa1095960:intron 35.0%
TCATGTAACCCTATAAAGCT+TGG - chr7_3:19562939-19562958 None:intergenic 35.0%
GGTTACATGAGAATAAAGAG+TGG + chr7_3:19562949-19562968 Msa1095960:intron 35.0%
TTCTTGATATTTAGGCAGCA+AGG + chr7_3:19563044-19563063 Msa1095960:intron 35.0%
TTTAAAGGGAGGAATGAATC+AGG + chr7_3:19563079-19563098 Msa1095960:CDS 35.0%
!! TCCACGATTTCTTAATTTGC+AGG + chr7_3:19563181-19563200 Msa1095960:intron 35.0%
!! TTTCTTAATTTGCAGGCAGA+AGG + chr7_3:19563188-19563207 Msa1095960:intron 35.0%
TGGTTTACCAATAAAGCAAG+TGG - chr7_3:19563227-19563246 None:intergenic 35.0%
CATGTGATAAACACAGTAAC+AGG + chr7_3:19563260-19563279 Msa1095960:CDS 35.0%
CTCATAAATGGATGCACTAA+TGG + chr7_3:19563500-19563519 Msa1095960:three_prime_UTR 35.0%
!! GTTTTTGTCTAGAACTCTTC+TGG + chr7_3:19563685-19563704 Msa1095960:three_prime_UTR 35.0%
TTGTTATGGAGCAGTTAGTG+CGG + chr7_3:19559776-19559795 Msa1095960:CDS 40.0%
ATACATGTTGGACAATGCAC+GGG + chr7_3:19559844-19559863 Msa1095960:CDS 40.0%
GAAGAAGAAACTGAGTTTGC+TGG + chr7_3:19559867-19559886 Msa1095960:intron 40.0%
!! AAACTGAGTTTGCTGGTTTG+AGG + chr7_3:19559874-19559893 Msa1095960:intron 40.0%
! ACTTAAAAGCACGCAATGGA+TGG + chr7_3:19560081-19560100 Msa1095960:intron 40.0%
AACAGCACACTCCCAAAAAT+AGG - chr7_3:19560330-19560349 None:intergenic 40.0%
!! ATTTTTGGGAGTGTGCTGTT+TGG + chr7_3:19560330-19560349 Msa1095960:intron 40.0%
!! GTGCTGTTTGTTCTGTTTCA+AGG + chr7_3:19560372-19560391 Msa1095960:intron 40.0%
GAAAATTTGATCAAGCCTCG+TGG + chr7_3:19560404-19560423 Msa1095960:intron 40.0%
!! AACTTATTTTCCAACCCACG+AGG - chr7_3:19560422-19560441 None:intergenic 40.0%
TATCCTCGTGAGATACATGT+GGG - chr7_3:19560457-19560476 None:intergenic 40.0%
TTATCCTCGTGAGATACATG+TGG - chr7_3:19560458-19560477 None:intergenic 40.0%
GTATCTCACGAGGATAAACA+AGG + chr7_3:19560461-19560480 Msa1095960:intron 40.0%
ATGGATGCAGGCTAAATCAT+TGG + chr7_3:19560643-19560662 Msa1095960:intron 40.0%
TGGATGCAGGCTAAATCATT+GGG + chr7_3:19560644-19560663 Msa1095960:intron 40.0%
TTATATGGATTCATTGCCGG+GGG + chr7_3:19560704-19560723 Msa1095960:intron 40.0%
!! CATTAGTTTGCATAGGACAC+TGG - chr7_3:19561027-19561046 None:intergenic 40.0%
CAGTGTCCTATGCAAACTAA+TGG + chr7_3:19561025-19561044 Msa1095960:intron 40.0%
!! CAAACTAATGGCATTGACTG+TGG + chr7_3:19561037-19561056 Msa1095960:intron 40.0%
ATACACTACAAAAAACGCGC+TGG + chr7_3:19561428-19561447 Msa1095960:intron 40.0%
CAATGAGTGAATCAAAGGAG+AGG - chr7_3:19561649-19561668 None:intergenic 40.0%
!! AAAGAAGTTCTGGAGAGTGA+CGG - chr7_3:19561794-19561813 None:intergenic 40.0%
!! GAGTGTTGGAAAAGAAGTTC+TGG - chr7_3:19561804-19561823 None:intergenic 40.0%
TTATGGCTGGTGATAGCTAT+TGG - chr7_3:19562153-19562172 None:intergenic 40.0%
ATCCTGAGAGAATACCAGTA+TGG - chr7_3:19562692-19562711 None:intergenic 40.0%
ATACTGGTATTCTCTCAGGA+TGG + chr7_3:19562691-19562710 Msa1095960:intron 40.0%
GTATCAACACCATATACCAG+GGG + chr7_3:19562729-19562748 Msa1095960:intron 40.0%
TAACTAACTCCCCTGGTATA+TGG - chr7_3:19562741-19562760 None:intergenic 40.0%
CATCCAAATGCATATTGGAC+TGG + chr7_3:19562897-19562916 Msa1095960:intron 40.0%
! ACAAGTAGGCCAAGCTTTAT+AGG + chr7_3:19562927-19562946 Msa1095960:intron 40.0%
! CAAGTAGGCCAAGCTTTATA+GGG + chr7_3:19562928-19562947 Msa1095960:intron 40.0%
TATTTAGGCAGCAAGGCAAT+TGG + chr7_3:19563051-19563070 Msa1095960:intron 40.0%
GCCTGCAAATTAAGAAATCG+TGG - chr7_3:19563185-19563204 None:intergenic 40.0%
TGCATCTCCACTTGCTTTAT+TGG + chr7_3:19563217-19563236 Msa1095960:CDS 40.0%
TCACATGCGAACTTGATCTT+TGG - chr7_3:19563247-19563266 None:intergenic 40.0%
CTAGACAAAAACCTTTGCAG+TGG - chr7_3:19563677-19563696 None:intergenic 40.0%
ATATAGGATTCCTGGTGAAG+TGG + chr7_3:19563783-19563802 Msa1095960:three_prime_UTR 40.0%
TACATGTTGGACAATGCACG+GGG + chr7_3:19559845-19559864 Msa1095960:CDS 45.0%
! CTAGGGAGCACATTATTCTC+AGG - chr7_3:19560155-19560174 None:intergenic 45.0%
TAGCCTCATAGGTTCGACTA+GGG - chr7_3:19560172-19560191 None:intergenic 45.0%
TTAGCCTCATAGGTTCGACT+AGG - chr7_3:19560173-19560192 None:intergenic 45.0%
ATCCTCGTGAGATACATGTG+GGG - chr7_3:19560456-19560475 None:intergenic 45.0%
!! GTGTTGCTTGTCATCAATCC+CGG + chr7_3:19560666-19560685 Msa1095960:intron 45.0%
TATATGGATTCATTGCCGGG+GGG + chr7_3:19560705-19560724 Msa1095960:intron 45.0%
AGATAAGCTAGTTGAGGTCG+AGG + chr7_3:19561263-19561282 Msa1095960:intron 45.0%
ACAAAAAACGCGCTGGATTG+CGG + chr7_3:19561435-19561454 Msa1095960:intron 45.0%
!!! GGATTGCGGAGGTTTTTTTG+CGG + chr7_3:19561449-19561468 Msa1095960:intron 45.0%
AAAGTGCGCTACACGTGAAA+GGG - chr7_3:19561614-19561633 None:intergenic 45.0%
! AAAAGTGCGCTACACGTGAA+AGG - chr7_3:19561615-19561634 None:intergenic 45.0%
ACCTCTCTTTCTCACTCTCA+CGG + chr7_3:19561753-19561772 Msa1095960:intron 45.0%
ACAACGAAGAGAGTGACAGA+GGG - chr7_3:19561834-19561853 None:intergenic 45.0%
GAATGGAACGATGAGAGTGA+CGG - chr7_3:19561914-19561933 None:intergenic 45.0%
ACTATGTCAATTAGGAGCCC+TGG + chr7_3:19562578-19562597 Msa1095960:intron 45.0%
ACTCACTGTTCATGCCATAC+TGG + chr7_3:19562675-19562694 Msa1095960:intron 45.0%
TGCCATACTGGTATTCTCTC+AGG + chr7_3:19562687-19562706 Msa1095960:intron 45.0%
AATAGGAAGTGAGAGATGCG+AGG - chr7_3:19562786-19562805 None:intergenic 45.0%
!! AGCTTTGAAGTGAAGGGGAA+GGG - chr7_3:19562814-19562833 None:intergenic 45.0%
!! TGTGGCAGCTTTGAAGTGAA+GGG - chr7_3:19562820-19562839 None:intergenic 45.0%
! TAATTTGCAGGCAGAAGGCT+TGG + chr7_3:19563193-19563212 Msa1095960:intron 45.0%
TATGACTAGAGTGCACCTGT+TGG - chr7_3:19563289-19563308 None:intergenic 45.0%
CTTCAGAACATACTTGCCAC+AGG + chr7_3:19563648-19563667 Msa1095960:intron 45.0%
! TGTCTAGAACTCTTCTGGGA+TGG + chr7_3:19563690-19563709 Msa1095960:three_prime_UTR 45.0%
AAGAAGTTAGCCACTTCACC+AGG - chr7_3:19563796-19563815 None:intergenic 45.0%
GGTGAAGTGGCTAACTTCTT+TGG + chr7_3:19563796-19563815 Msa1095960:three_prime_UTR 45.0%
!!! GTTATTTTATTTTTTAATTT+AGG + chr7_3:19563958-19563977 Msa1095960:three_prime_UTR 5.0%
! GCGTCAAAGGAGTACAGAGA+AGG + chr7_3:19559678-19559697 Msa1095960:CDS 50.0%
TTTGATCAAGCCTCGTGGGT+TGG + chr7_3:19560409-19560428 Msa1095960:intron 50.0%
ATCCCCACATGTATCTCACG+AGG + chr7_3:19560451-19560470 Msa1095960:intron 50.0%
TCAATGTTAGGATGTCCCCC+CGG - chr7_3:19560723-19560742 None:intergenic 50.0%
AAGGGAGCGGCAAATACACA+TGG - chr7_3:19560858-19560877 None:intergenic 50.0%
!! TTGACTTGGGATGAAGGGAG+CGG - chr7_3:19560871-19560890 None:intergenic 50.0%
TAAGCTAGTTGAGGTCGAGG+AGG + chr7_3:19561266-19561285 Msa1095960:intron 50.0%
TAGTTGAGGTCGAGGAGGAT+TGG + chr7_3:19561271-19561290 Msa1095960:intron 50.0%
AGTTGAGGTCGAGGAGGATT+GGG + chr7_3:19561272-19561291 Msa1095960:intron 50.0%
GGAGGATTGGGCAACCTTTA+TGG + chr7_3:19561284-19561303 Msa1095960:intron 50.0%
!!! TTGCGGAGGTTTTTTTGCGG+AGG + chr7_3:19561452-19561471 Msa1095960:intron 50.0%
!! GAGTGAGAAGGTTCTGGAGA+TGG - chr7_3:19561711-19561730 None:intergenic 50.0%
GAGAGAGAGTGAGAAGGTTC+TGG - chr7_3:19561717-19561736 None:intergenic 50.0%
GAGTGAGAAAGAGAGGTTCC+TGG - chr7_3:19561750-19561769 None:intergenic 50.0%
GACAACGAAGAGAGTGACAG+AGG - chr7_3:19561835-19561854 None:intergenic 50.0%
GCAACGGCTACAGTAACAGA+GGG - chr7_3:19561861-19561880 None:intergenic 50.0%
TGCAACGGCTACAGTAACAG+AGG - chr7_3:19561862-19561881 None:intergenic 50.0%
! AGTGATGGAGCGGTTTGCAA+CGG - chr7_3:19561877-19561896 None:intergenic 50.0%
! AGAGAGTGAGAGTGATGGAG+CGG - chr7_3:19561887-19561906 None:intergenic 50.0%
! TGAGGGAGACAGTGATGGAA+TGG - chr7_3:19561931-19561950 None:intergenic 50.0%
CTCTATGAGGGAGACAGTGA+TGG - chr7_3:19561936-19561955 None:intergenic 50.0%
CATCACTGTCTCCCTCATAG+AGG + chr7_3:19561934-19561953 Msa1095960:intron 50.0%
!! CAGCTTTGAAGTGAAGGGGA+AGG - chr7_3:19562815-19562834 None:intergenic 50.0%
!! GTGGCAGCTTTGAAGTGAAG+GGG - chr7_3:19562819-19562838 None:intergenic 50.0%
!! GTGTGGCAGCTTTGAAGTGA+AGG - chr7_3:19562821-19562840 None:intergenic 50.0%
CACAGTAACAGGCTTCCAAC+AGG + chr7_3:19563271-19563290 Msa1095960:CDS 50.0%
TAGCGCACTCATTGGAGGAA+GGG - chr7_3:19563527-19563546 None:intergenic 50.0%
TTAGCGCACTCATTGGAGGA+AGG - chr7_3:19563528-19563547 None:intergenic 50.0%
TCCTCCAATGAGTGCGCTAA+TGG + chr7_3:19563528-19563547 Msa1095960:three_prime_UTR 50.0%
TCCATTAGCGCACTCATTGG+AGG - chr7_3:19563532-19563551 None:intergenic 50.0%
GTCTCCATTAGCGCACTCAT+TGG - chr7_3:19563535-19563554 None:intergenic 50.0%
ACCTTTGCAGTGGAAACCTG+TGG - chr7_3:19563667-19563686 None:intergenic 50.0%
GTCTGCGAATCGAGCGTCAA+AGG + chr7_3:19559665-19559684 Msa1095960:CDS 55.0%
GCTCCCTAGTCGAACCTATG+AGG + chr7_3:19560166-19560185 Msa1095960:intron 55.0%
AAAAACGCGCTGGATTGCGG+AGG + chr7_3:19561438-19561457 Msa1095960:intron 55.0%
!! ACGTGAAAGGGTAGCGGGAT+AGG - chr7_3:19561602-19561621 None:intergenic 55.0%
GCTACACGTGAAAGGGTAGC+GGG - chr7_3:19561607-19561626 None:intergenic 55.0%
CGCTACACGTGAAAGGGTAG+CGG - chr7_3:19561608-19561627 None:intergenic 55.0%
GTGAGGGAGAGAGAGTGAGA+AGG - chr7_3:19561723-19561742 None:intergenic 55.0%
AGAGGTTCCTGGAGCTGTGA+GGG - chr7_3:19561739-19561758 None:intergenic 55.0%
GCCGTGAGAGTGAGAAAGAG+AGG - chr7_3:19561757-19561776 None:intergenic 55.0%
!! GAAGTTCTGGAGAGTGACGG+CGG - chr7_3:19561791-19561810 None:intergenic 55.0%
CAGAGGGAGAGAGTGAGTGT+TGG - chr7_3:19561818-19561837 None:intergenic 55.0%
GAGGGAGAGAGTGAGAGTGA+TGG - chr7_3:19561892-19561911 None:intergenic 55.0%
! GGAACGATGAGAGTGACGGA+GGG - chr7_3:19561910-19561929 None:intergenic 55.0%
TGGAACGATGAGAGTGACGG+AGG - chr7_3:19561911-19561930 None:intergenic 55.0%
GAAGGGGAAGGGACAGGAAT+AGG - chr7_3:19562803-19562822 None:intergenic 55.0%
TGAAGTGAAGGGGAAGGGAC+AGG - chr7_3:19562809-19562828 None:intergenic 55.0%
GCCACAGGTTTCCACTGCAA+AGG + chr7_3:19563663-19563682 Msa1095960:intron 55.0%
TCTCTCTCCCTCACAGCTCC+AGG + chr7_3:19561729-19561748 Msa1095960:intron 60.0%
GAGAGGTTCCTGGAGCTGTG+AGG - chr7_3:19561740-19561759 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 19559648 19564088 19559648 ID=Msa1095960;Name=Msa1095960
chr7_3 mRNA 19559648 19564088 19559648 ID=Msa1095960-mRNA-1;Parent=Msa1095960;Name=Msa1095960-mRNA-1;_AED=0.37;_eAED=0.37;_QI=0|0.5|0.6|0.8|0.25|0.4|5|638|176
chr7_3 exon 19559648 19559880 19559648 ID=Msa1095960-mRNA-1:exon:4230;Parent=Msa1095960-mRNA-1
chr7_3 exon 19560853 19560928 19560853 ID=Msa1095960-mRNA-1:exon:4231;Parent=Msa1095960-mRNA-1
chr7_3 exon 19563058 19563123 19563058 ID=Msa1095960-mRNA-1:exon:4232;Parent=Msa1095960-mRNA-1
chr7_3 exon 19563203 19563577 19563203 ID=Msa1095960-mRNA-1:exon:4233;Parent=Msa1095960-mRNA-1
chr7_3 exon 19563670 19564088 19563670 ID=Msa1095960-mRNA-1:exon:4234;Parent=Msa1095960-mRNA-1
chr7_3 CDS 19559648 19559880 19559648 ID=Msa1095960-mRNA-1:cds;Parent=Msa1095960-mRNA-1
chr7_3 CDS 19560853 19560928 19560853 ID=Msa1095960-mRNA-1:cds;Parent=Msa1095960-mRNA-1
chr7_3 CDS 19563058 19563123 19563058 ID=Msa1095960-mRNA-1:cds;Parent=Msa1095960-mRNA-1
chr7_3 CDS 19563203 19563358 19563203 ID=Msa1095960-mRNA-1:cds;Parent=Msa1095960-mRNA-1
chr7_3 three_prime_UTR 19563359 19563577 19563359 ID=Msa1095960-mRNA-1:three_prime_utr;Parent=Msa1095960-mRNA-1
chr7_3 three_prime_UTR 19563670 19564088 19563670 ID=Msa1095960-mRNA-1:three_prime_utr;Parent=Msa1095960-mRNA-1
Gene Sequence

>Msa1095960

ATGGATCGGAAATGGGTGTCTGCGAATCGAGCGTCAAAGGAGTACAGAGAAGGAGTGAAAGAGTTTGTTGAGTTTGTTGTTGCTCATGCAAAAGACAGCAATAACATCATATGCCCATGCTTAGAATGTTGTTATGGAGCAGTTAGTGCGGAGCATTTGCAAGATCACTTAGTATGTAATGGAATTGATGAAAGCTATACATGTTGGACAATGCACGGGGAAGAAGAAACTGATGCCATGTGTATTTGCCGCTCCCTTCATCCCAAGTCAAAATCAAAGTCAAGTATAATCCCATGGATAGAAATAAAGGCAGCAAGGCAATTGGAATTTTTTAAAGGGAGGAATGAATCAGGAATTTCAATAGGCTATGATGAGGCAGAAGGCTTGGTTGCATCTCCACTTGCTTTATTGGTAAACCAAAGATCAAGTTCGCATGTGATAAACACAGTAACAGGCTTCCAACAGGTGCACTCTAGTCATAGAATTCATTTATCTCCTTTTTTGTTTTTCTTTTTGCAAGAAATCCATTAA
Protein sequence

>Msa1095960

MDRKWVSANRASKEYREGVKEFVEFVVAHAKDSNNIICPCLECCYGAVSAEHLQDHLVCNGIDESYTCWTMHGEEETDAMCICRSLHPKSKSKSSIIPWIEIKAARQLEFFKGRNESGISIGYDEAEGLVASPLALLVNQRSSSHVINTVTGFQQVHSSHRIHLSPFLFFFLQEIH*