AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0746220

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0746220 MtrunA17_Chr6g0457561 97.024 168 5 0 1 168 1 168 2.16e-119 334
Msa0746220 MtrunA17_Chr8g0391011 49.669 151 65 4 28 168 11 160 4.27e-35 120
Msa0746220 MtrunA17_Chr1g0209811 46.203 158 68 5 27 168 4 160 1.56e-31 111
Msa0746220 MtrunA17_Chr6g0484481 42.697 178 81 7 1 168 1 167 3.51e-30 108
Msa0746220 MtrunA17_Chr4g0045631 44.318 176 55 5 1 168 1 141 1.96e-28 102
Msa0746220 MtrunA17_Chr1g0171841 43.243 185 57 6 1 168 1 154 2.40e-28 102
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0746220 AT5G16110.1 53.005 183 66 5 1 168 67 244 4.51e-50 161
Msa0746220 AT3G02555.2 54.335 173 63 6 1 168 1 162 2.37e-44 144
Msa0746220 AT3G02555.1 54.335 173 63 6 1 168 1 162 2.37e-44 144
Msa0746220 AT1G68490.1 46.486 185 80 9 1 168 1 183 2.13e-40 135
Msa0746220 AT1G13390.1 41.111 180 89 7 1 168 2 176 5.92e-32 113
Msa0746220 AT1G13390.2 41.111 180 89 7 1 168 2 176 5.92e-32 113

Find 73 sgRNAs with CRISPR-Local

Find 155 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTTGCGGGATGCAGAATT+TGG 0.163389 5_1:-68375309 None:intergenic
ATGGCTTATTATATATTTAA+TGG 0.180904 5_1:+68375761 Msa0746220:three_prime_UTR
GTATAGAGAGTTGGTGATTT+TGG 0.220921 5_1:+68375699 Msa0746220:three_prime_UTR
TGCATGACCTAATGGTTTAT+AGG 0.293597 5_1:+68375821 Msa0746220:three_prime_UTR
CAGCAGGGACGCTTGAATTC+TGG 0.294975 5_1:-68375410 None:intergenic
CCGGCTGCCGTTAGAGTAGA+AGG 0.314222 5_1:+68375363 Msa0746220:CDS
CCTAACACATCCACCTTTGC+GGG 0.345952 5_1:-68375322 None:intergenic
TTGGTCCAGGATGCTCGATT+TGG 0.359597 5_1:+68375246 Msa0746220:CDS
TTTCCGTGTCTCCACCGGTC+AGG 0.370731 5_1:+68375211 Msa0746220:CDS
AAGGGTTCGACTGCCTCAAC+AGG 0.387499 5_1:+68375382 Msa0746220:CDS
AATTGATCCTATAAACCATT+AGG 0.396624 5_1:-68375828 None:intergenic
GGATGTGTTAGGATGAGTTT+TGG 0.398776 5_1:+68375333 Msa0746220:CDS
AAAATATCTTGAGTAGTTTG+AGG 0.405685 5_1:+68375535 Msa0746220:three_prime_UTR
ATGCCATTTGCATGACCTAA+TGG 0.405687 5_1:+68375813 Msa0746220:three_prime_UTR
GTCCTAAGCCACGACGAGTC+GGG 0.412417 5_1:+68374006 Msa0746220:CDS
TTTATATACACTTCTCTGAA+TGG 0.415088 5_1:-68375445 None:intergenic
TTCCGTGTCTCCACCGGTCA+GGG 0.433361 5_1:+68375212 Msa0746220:CDS
CCCGCAAAGGTGGATGTGTT+AGG 0.445900 5_1:+68375322 Msa0746220:CDS
GAGAGAGAGATTAACCTTGA+GGG 0.463059 5_1:-68373102 None:intergenic
GTAGTTTGAGGTTATAATGT+TGG 0.465141 5_1:+68375547 Msa0746220:three_prime_UTR
TCCTAACACATCCACCTTTG+CGG 0.472579 5_1:-68375323 None:intergenic
TCCTTCAACAGGAGAGTCAC+GGG 0.477973 5_1:+68375148 Msa0746220:intron
AACGGTTCATCTTCGATGCC+AGG 0.479659 5_1:-68373899 None:intergenic
ATTTAATGGATTAATTAAAG+TGG 0.479661 5_1:+68375775 Msa0746220:three_prime_UTR
CAAACCTTGTGTTCTTGAGA+AGG 0.481678 5_1:+68375576 Msa0746220:three_prime_UTR
GGCTGCAAATCCCTTGGTCC+AGG 0.494860 5_1:+68375233 Msa0746220:CDS
TTGGAGATGGCAAGCCTGAT+GGG 0.496255 5_1:-68375290 None:intergenic
CGGGATGCAGAATTTGGAGA+TGG 0.500725 5_1:-68375303 None:intergenic
CCTTCTACTCTAACGGCAGC+CGG 0.501686 5_1:-68375363 None:intergenic
TTTGTTTGATCTCTCCCTCA+AGG 0.503206 5_1:+68373088 Msa0746220:five_prime_UTR
CGTTTCCAAATCGAGCATCC+TGG 0.506071 5_1:-68375251 None:intergenic
ATCATATCATCATCCCCATC+AGG 0.509951 5_1:+68375276 Msa0746220:CDS
CAAAGTAGCTGGCTGCTACA+AGG 0.514858 5_1:-68373069 None:intergenic
CATCTCTTCACAAGCTGCAA+AGG 0.522362 5_1:-68373940 None:intergenic
AAACCATTAGGTCATGCAAA+TGG 0.527081 5_1:-68375816 None:intergenic
ATGGAATGTTAAGCGACAGC+AGG 0.533461 5_1:-68375426 None:intergenic
GAGAGATCAAACAAAGTAGC+TGG 0.533961 5_1:-68373080 None:intergenic
GGTCAGGGCTGCAAATCCCT+TGG 0.539999 5_1:+68375227 Msa0746220:CDS
TTCCTTCAACAGGAGAGTCA+CGG 0.541824 5_1:+68375147 Msa0746220:intron
TGAAATCTCAATTGTCGCAC+AGG 0.546029 5_1:-68374044 None:intergenic
TTTGGAGATGGCAAGCCTGA+TGG 0.546888 5_1:-68375291 None:intergenic
TCCTAAGCCACGACGAGTCG+GGG 0.547817 5_1:+68374007 Msa0746220:CDS
TCTCTATACATTCATTCTCA+TGG 0.548602 5_1:-68375685 None:intergenic
TGTTCATAGTCAACAAGCTG+AGG 0.552611 5_1:+68374158 Msa0746220:intron
AGCCCTGACCGGTGGAGACA+CGG 0.557082 5_1:-68375214 None:intergenic
GTTCATAGTCAACAAGCTGA+GGG 0.558392 5_1:+68374159 Msa0746220:intron
TGTTCTTAGGCGTTCTACTG+TGG 0.558532 5_1:+68373868 Msa0746220:intron
AATTCTGGCGATCCCTGTTG+AGG 0.559016 5_1:-68375395 None:intergenic
TCTACTGTGGAATAAGAACC+TGG 0.564324 5_1:+68373881 Msa0746220:five_prime_UTR
GTCGAACCCTTCTACTCTAA+CGG 0.572882 5_1:-68375370 None:intergenic
AATTCTGCATCCCGCAAAGG+TGG 0.573238 5_1:+68375312 Msa0746220:CDS
AGGCTCCTTGTGATCAGTAA+TGG 0.576308 5_1:-68373976 None:intergenic
CAAACCTTCTCAAGAACACA+AGG 0.580335 5_1:-68375580 None:intergenic
AGAATGAATGTATAGAGAGT+TGG 0.581329 5_1:+68375690 Msa0746220:three_prime_UTR
CAGAGAAGTGTATATAAAGA+CGG 0.583993 5_1:+68375450 Msa0746220:three_prime_UTR
TGTCCTAAGCCACGACGAGT+CGG 0.584427 5_1:+68374005 Msa0746220:CDS
CCAAATTCTGCATCCCGCAA+AGG 0.597293 5_1:+68375309 Msa0746220:CDS
GTAGCTGGCTGCTACAAGGA+AGG 0.600650 5_1:-68373065 None:intergenic
AAATCGAGCATCCTGGACCA+AGG 0.614921 5_1:-68375244 None:intergenic
TGGAATGTTAAGCGACAGCA+GGG 0.618138 5_1:-68375425 None:intergenic
GCAGCTTGTGAAGAGATGAG+AGG 0.621316 5_1:+68373945 Msa0746220:CDS
GGATTTGCAGCCCTGACCGG+TGG 0.621974 5_1:-68375222 None:intergenic
GACAAGACCCCGACTCGTCG+TGG 0.623519 5_1:-68374014 None:intergenic
CGTTCTGCTGAAGACTGTAA+CGG 0.626857 5_1:-68373917 None:intergenic
AATCGAGCATCCTGGACCAA+GGG 0.628072 5_1:-68375243 None:intergenic
TGGCTGCTACAAGGAAGGAG+AGG 0.636394 5_1:-68373060 None:intergenic
GGGTTCTGATTCAAAAGCTG+CGG 0.642823 5_1:+68374179 Msa0746220:CDS
AGTTACCATTACTGATCACA+AGG 0.652918 5_1:+68373971 Msa0746220:CDS
TGGAGATGGCAAGCCTGATG+GGG 0.657463 5_1:-68375289 None:intergenic
CGGCTGCCGTTAGAGTAGAA+GGG 0.675818 5_1:+68375364 Msa0746220:CDS
CCTTCAACAGGAGAGTCACG+GGG 0.682882 5_1:+68375149 Msa0746220:intron
AGGGTTCGACTGCCTCAACA+GGG 0.685342 5_1:+68375383 Msa0746220:CDS
CGTGGCTTAGGACAAATGAG+AGG 0.704524 5_1:-68373996 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTATTTTTATATATTT+TGG + chr5_1:68373319-68373338 Msa0746220:intron 0.0%
!!! TTTTTTATGTTTGTTATTTT+TGG + chr5_1:68373376-68373395 Msa0746220:intron 10.0%
!!! ATTTGTTATAATCTTTTATT+AGG + chr5_1:68373633-68373652 Msa0746220:intron 10.0%
!!! TTTATTTTGATTTTGTTAAA+TGG + chr5_1:68374731-68374750 Msa0746220:intron 10.0%
!!! TCTTTAATTTATACTGTTTT+TGG + chr5_1:68374656-68374675 Msa0746220:intron 15.0%
!!! CTTTAATTTATACTGTTTTT+GGG + chr5_1:68374657-68374676 Msa0746220:intron 15.0%
!! CATTAAAATACTAAAATGAT+AGG - chr5_1:68374810-68374829 None:intergenic 15.0%
!!! TATTTTAATGTTCTGATTTT+TGG + chr5_1:68374820-68374839 Msa0746220:intron 15.0%
!!! GTGACTTAAATAATTTTTTT+AGG + chr5_1:68375656-68375675 Msa0746220:three_prime_UTR 15.0%
!! TTTAGAAAAATTTATGAGTA+TGG + chr5_1:68375742-68375761 Msa0746220:three_prime_UTR 15.0%
!! ATGGCTTATTATATATTTAA+TGG + chr5_1:68375761-68375780 Msa0746220:three_prime_UTR 15.0%
!!! ATTTAATGGATTAATTAAAG+TGG + chr5_1:68375775-68375794 Msa0746220:three_prime_UTR 15.0%
!!! TATATTTTGGATGATAATTG+AGG + chr5_1:68373332-68373351 Msa0746220:intron 20.0%
!!! TGGAAATTTTTTTGTTTTGT+CGG + chr5_1:68373402-68373421 Msa0746220:intron 20.0%
!!! AAAAGTTTGAGAATTTTTCA+AGG + chr5_1:68373477-68373496 Msa0746220:intron 20.0%
!! AATTTGTGAAAGATTGAAAA+AGG + chr5_1:68373562-68373581 Msa0746220:intron 20.0%
!!! TTTAGTTTTTTAGTGTAATG+TGG + chr5_1:68373605-68373624 Msa0746220:intron 20.0%
!! AAACAAACATAACAAAATCA+TGG - chr5_1:68374924-68374943 None:intergenic 20.0%
!!! AATTAATCGAAGTTTGAAAT+CGG - chr5_1:68375115-68375134 None:intergenic 20.0%
!!! TTCTTTTTTTGTGTACTAAA+AGG + chr5_1:68375619-68375638 Msa0746220:three_prime_UTR 20.0%
!!! ATAGGATCAATTTTGATATT+TGG + chr5_1:68375839-68375858 Msa0746220:three_prime_UTR 20.0%
! TGTAGTGAGTGTAAAAAAAA+GGG - chr5_1:68372982-68373001 None:intergenic 25.0%
! TTGTAGTGAGTGTAAAAAAA+AGG - chr5_1:68372983-68373002 None:intergenic 25.0%
! AACAAAAGATGTTACAAACA+TGG - chr5_1:68373176-68373195 None:intergenic 25.0%
!! AATTCTCAAACTTTTCATCT+GGG - chr5_1:68373472-68373491 None:intergenic 25.0%
!! AAATTCTCAAACTTTTCATC+TGG - chr5_1:68373473-68373492 None:intergenic 25.0%
!! TTTAAGAAATTTTCTGCAGA+TGG + chr5_1:68373665-68373684 Msa0746220:intron 25.0%
! ATTGACATTGAATATTGTTC+TGG + chr5_1:68374094-68374113 Msa0746220:intron 25.0%
! CCTATTTCATAAAGATAACT+GGG - chr5_1:68374531-68374550 None:intergenic 25.0%
! TCCTATTTCATAAAGATAAC+TGG - chr5_1:68374532-68374551 None:intergenic 25.0%
! TAAAGATAACCAAGCAAAAA+AGG - chr5_1:68374642-68374661 None:intergenic 25.0%
!! ATGATTAGTAATTTTCCTGT+AGG + chr5_1:68374978-68374997 Msa0746220:intron 25.0%
! TTTATATACACTTCTCTGAA+TGG - chr5_1:68375448-68375467 None:intergenic 25.0%
! AAAATATCTTGAGTAGTTTG+AGG + chr5_1:68375535-68375554 Msa0746220:three_prime_UTR 25.0%
! AATTGATCCTATAAACCATT+AGG - chr5_1:68375831-68375850 None:intergenic 25.0%
GTAGTGAGTGTAAAAAAAAG+GGG - chr5_1:68372981-68373000 None:intergenic 30.0%
TGAAACATAACATGTTTCCT+AGG + chr5_1:68373230-68373249 Msa0746220:intron 30.0%
!!! ATGTTTGTTATTTTTGGCGA+TGG + chr5_1:68373382-68373401 Msa0746220:intron 30.0%
!! GTCGGTTGATTTTAATGTAA+AGG + chr5_1:68373420-68373439 Msa0746220:intron 30.0%
TTCACAAAACCTACACAAAA+GGG - chr5_1:68373544-68373563 None:intergenic 30.0%
ATTCACAAAACCTACACAAA+AGG - chr5_1:68373545-68373564 None:intergenic 30.0%
! TTGTTGTGTTTTGTGTTCTT+AGG + chr5_1:68373855-68373874 Msa0746220:intron 30.0%
AATTTGTCGAGCTCATATTT+AGG + chr5_1:68374251-68374270 Msa0746220:intron 30.0%
AAATGTCTGTGTGATAATCT+TGG + chr5_1:68374289-68374308 Msa0746220:intron 30.0%
CCCAGTTATCTTTATGAAAT+AGG + chr5_1:68374528-68374547 Msa0746220:intron 30.0%
!! CTTATTAACGAAGGCATTAA+TGG - chr5_1:68374683-68374702 None:intergenic 30.0%
TGTTCTCTTAGAAAAAGAGT+TGG + chr5_1:68374756-68374775 Msa0746220:intron 30.0%
! GGATTCGTCTTATTATTAGT+CGG + chr5_1:68374777-68374796 Msa0746220:intron 30.0%
CAAAAATCACATTTACCTAC+AGG - chr5_1:68374996-68375015 None:intergenic 30.0%
! CTGTAGGTAAATGTGATTTT+TGG + chr5_1:68374994-68375013 Msa0746220:intron 30.0%
! CAGAGAAGTGTATATAAAGA+CGG + chr5_1:68375450-68375469 Msa0746220:three_prime_UTR 30.0%
! GTAGTTTGAGGTTATAATGT+TGG + chr5_1:68375547-68375566 Msa0746220:three_prime_UTR 30.0%
TCTCTATACATTCATTCTCA+TGG - chr5_1:68375688-68375707 None:intergenic 30.0%
AGAATGAATGTATAGAGAGT+TGG + chr5_1:68375690-68375709 Msa0746220:three_prime_UTR 30.0%
CAATTGAACAGATCTATCCT+AGG - chr5_1:68373250-68373269 None:intergenic 35.0%
!! ATTAAGTTGCCCTTTTGTGT+AGG + chr5_1:68373532-68373551 Msa0746220:intron 35.0%
AGTTACCATTACTGATCACA+AGG + chr5_1:68373971-68373990 Msa0746220:CDS 35.0%
!! TATTGTTCTGGATGCCTTTT+AGG + chr5_1:68374106-68374125 Msa0746220:intron 35.0%
!! GCTTGACATGATTTTTGAGA+AGG + chr5_1:68374209-68374228 Msa0746220:CDS 35.0%
TCAAATTACACTGCACTACT+AGG - chr5_1:68374318-68374337 None:intergenic 35.0%
TAAAGATAACTGGGCAAATC+AGG - chr5_1:68374522-68374541 None:intergenic 35.0%
AAATAGGATTGCGAGATTCT+TGG + chr5_1:68374544-68374563 Msa0746220:intron 35.0%
! AATAAGACACACGGTTTGTT+TGG + chr5_1:68374697-68374716 Msa0746220:intron 35.0%
!!! TATGTTTTGATGCACGGTTA+AGG + chr5_1:68374871-68374890 Msa0746220:intron 35.0%
CTAATCATATTCAACAAGCG+TGG - chr5_1:68374966-68374985 None:intergenic 35.0%
CTTCTAGTCAAACTGAGATT+TGG - chr5_1:68375057-68375076 None:intergenic 35.0%
! TTGCATTCTTTTCCTTCAAC+AGG + chr5_1:68375137-68375156 Msa0746220:intron 35.0%
!!! GATGAGTTTTGGACTTAAAC+CGG + chr5_1:68375344-68375363 Msa0746220:CDS 35.0%
!!! GTATAGAGAGTTGGTGATTT+TGG + chr5_1:68375699-68375718 Msa0746220:three_prime_UTR 35.0%
AAACCATTAGGTCATGCAAA+TGG - chr5_1:68375819-68375838 None:intergenic 35.0%
TGCATGACCTAATGGTTTAT+AGG + chr5_1:68375821-68375840 Msa0746220:three_prime_UTR 35.0%
GAGAGATCAAACAAAGTAGC+TGG - chr5_1:68373083-68373102 None:intergenic 40.0%
TTTGTTTGATCTCTCCCTCA+AGG + chr5_1:68373088-68373107 Msa0746220:five_prime_UTR 40.0%
GAGAGAGAGATTAACCTTGA+GGG - chr5_1:68373105-68373124 None:intergenic 40.0%
AGAGAGAGAGATTAACCTTG+AGG - chr5_1:68373106-68373125 None:intergenic 40.0%
TCTACTGTGGAATAAGAACC+TGG + chr5_1:68373881-68373900 Msa0746220:five_prime_UTR 40.0%
TGAAATCTCAATTGTCGCAC+AGG - chr5_1:68374047-68374066 None:intergenic 40.0%
TTACAGATCAGCCACCTAAA+AGG - chr5_1:68374123-68374142 None:intergenic 40.0%
TGTTCATAGTCAACAAGCTG+AGG + chr5_1:68374158-68374177 Msa0746220:intron 40.0%
GTTCATAGTCAACAAGCTGA+GGG + chr5_1:68374159-68374178 Msa0746220:intron 40.0%
GCTGCATATGTTGTCAATCA+TGG - chr5_1:68374392-68374411 None:intergenic 40.0%
GAACACGATTGCAAGAATCT+TGG + chr5_1:68374425-68374444 Msa0746220:intron 40.0%
TTGCAAGAATCTTGGATGAG+TGG + chr5_1:68374433-68374452 Msa0746220:intron 40.0%
! TACGTGTTTTCCGTGCATTT+TGG + chr5_1:68374490-68374509 Msa0746220:intron 40.0%
!!! TGGCGAATACCTTTTTTGCT+TGG + chr5_1:68374630-68374649 Msa0746220:intron 40.0%
GCCTTCGTTAATAAGACACA+CGG + chr5_1:68374688-68374707 Msa0746220:intron 40.0%
! ACCGTGTGTCTTATTAACGA+AGG - chr5_1:68374692-68374711 None:intergenic 40.0%
!!! ACCGTGTATGTTTTGATGCA+CGG + chr5_1:68374865-68374884 Msa0746220:intron 40.0%
ACCGTGCATCAAAACATACA+CGG - chr5_1:68374869-68374888 None:intergenic 40.0%
GGAAAAATATGGAGGAGATG+AGG - chr5_1:68375196-68375215 None:intergenic 40.0%
! ATATTTTTCCGTGTCTCCAC+CGG + chr5_1:68375206-68375225 Msa0746220:CDS 40.0%
ATCATATCATCATCCCCATC+AGG + chr5_1:68375276-68375295 Msa0746220:CDS 40.0%
! GGATGTGTTAGGATGAGTTT+TGG + chr5_1:68375333-68375352 Msa0746220:CDS 40.0%
CAAACCTTGTGTTCTTGAGA+AGG + chr5_1:68375576-68375595 Msa0746220:three_prime_UTR 40.0%
CAAACCTTCTCAAGAACACA+AGG - chr5_1:68375583-68375602 None:intergenic 40.0%
! ATGCCATTTGCATGACCTAA+TGG + chr5_1:68375813-68375832 Msa0746220:three_prime_UTR 40.0%
TGTTCTTAGGCGTTCTACTG+TGG + chr5_1:68373868-68373887 Msa0746220:intron 45.0%
CGTTCTGCTGAAGACTGTAA+CGG - chr5_1:68373920-68373939 None:intergenic 45.0%
CATCTCTTCACAAGCTGCAA+AGG - chr5_1:68373943-68373962 None:intergenic 45.0%
AGGCTCCTTGTGATCAGTAA+TGG - chr5_1:68373979-68373998 None:intergenic 45.0%
!! TGTTCTGGATGCCTTTTAGG+TGG + chr5_1:68374109-68374128 Msa0746220:intron 45.0%
! GGGTTCTGATTCAAAAGCTG+CGG + chr5_1:68374179-68374198 Msa0746220:CDS 45.0%
TCGAGCTCATATTTAGGCTC+AGG + chr5_1:68374257-68374276 Msa0746220:intron 45.0%
CAGGTATTCGCCAAAATGCA+CGG - chr5_1:68374503-68374522 None:intergenic 45.0%
TTGCGAGATTCTTGGATGAG+TGG + chr5_1:68374552-68374571 Msa0746220:intron 45.0%
TATGCACTTTCCGTGCATTC+TGG + chr5_1:68374610-68374629 Msa0746220:intron 45.0%
! AAGGTATTCGCCAGAATGCA+CGG - chr5_1:68374623-68374642 None:intergenic 45.0%
TTCCTTCAACAGGAGAGTCA+CGG + chr5_1:68375147-68375166 Msa0746220:intron 45.0%
GGAGACACGGAAAAATATGG+AGG - chr5_1:68375204-68375223 None:intergenic 45.0%
GGTGGAGACACGGAAAAATA+TGG - chr5_1:68375207-68375226 None:intergenic 45.0%
TCCTAACACATCCACCTTTG+CGG - chr5_1:68375326-68375345 None:intergenic 45.0%
GTCGAACCCTTCTACTCTAA+CGG - chr5_1:68375373-68375392 None:intergenic 45.0%
TGGAATGTTAAGCGACAGCA+GGG - chr5_1:68375428-68375447 None:intergenic 45.0%
ATGGAATGTTAAGCGACAGC+AGG - chr5_1:68375429-68375448 None:intergenic 45.0%
CAAAGTAGCTGGCTGCTACA+AGG - chr5_1:68373072-68373091 None:intergenic 50.0%
AACGGTTCATCTTCGATGCC+AGG - chr5_1:68373902-68373921 None:intergenic 50.0%
GCAGCTTGTGAAGAGATGAG+AGG + chr5_1:68373945-68373964 Msa0746220:CDS 50.0%
CGTGGCTTAGGACAAATGAG+AGG - chr5_1:68373999-68374018 None:intergenic 50.0%
TCCTTCAACAGGAGAGTCAC+GGG + chr5_1:68375148-68375167 Msa0746220:intron 50.0%
AATCGAGCATCCTGGACCAA+GGG - chr5_1:68375246-68375265 None:intergenic 50.0%
AAATCGAGCATCCTGGACCA+AGG - chr5_1:68375247-68375266 None:intergenic 50.0%
TTGGTCCAGGATGCTCGATT+TGG + chr5_1:68375246-68375265 Msa0746220:CDS 50.0%
CGTTTCCAAATCGAGCATCC+TGG - chr5_1:68375254-68375273 None:intergenic 50.0%
TTGGAGATGGCAAGCCTGAT+GGG - chr5_1:68375293-68375312 None:intergenic 50.0%
TTTGGAGATGGCAAGCCTGA+TGG - chr5_1:68375294-68375313 None:intergenic 50.0%
CGGGATGCAGAATTTGGAGA+TGG - chr5_1:68375306-68375325 None:intergenic 50.0%
CCTTTGCGGGATGCAGAATT+TGG - chr5_1:68375312-68375331 None:intergenic 50.0%
CCAAATTCTGCATCCCGCAA+AGG + chr5_1:68375309-68375328 Msa0746220:CDS 50.0%
AATTCTGCATCCCGCAAAGG+TGG + chr5_1:68375312-68375331 Msa0746220:CDS 50.0%
CCTAACACATCCACCTTTGC+GGG - chr5_1:68375325-68375344 None:intergenic 50.0%
AATTCTGGCGATCCCTGTTG+AGG - chr5_1:68375398-68375417 None:intergenic 50.0%
TGGCTGCTACAAGGAAGGAG+AGG - chr5_1:68373063-68373082 None:intergenic 55.0%
GTAGCTGGCTGCTACAAGGA+AGG - chr5_1:68373068-68373087 None:intergenic 55.0%
TGTCCTAAGCCACGACGAGT+CGG + chr5_1:68374005-68374024 Msa0746220:CDS 55.0%
CCTTCAACAGGAGAGTCACG+GGG + chr5_1:68375149-68375168 Msa0746220:intron 55.0%
AAGGGATTTGCAGCCCTGAC+CGG - chr5_1:68375228-68375247 None:intergenic 55.0%
TGGAGATGGCAAGCCTGATG+GGG - chr5_1:68375292-68375311 None:intergenic 55.0%
CCCGCAAAGGTGGATGTGTT+AGG + chr5_1:68375322-68375341 Msa0746220:CDS 55.0%
CCTTCTACTCTAACGGCAGC+CGG - chr5_1:68375366-68375385 None:intergenic 55.0%
CGGCTGCCGTTAGAGTAGAA+GGG + chr5_1:68375364-68375383 Msa0746220:CDS 55.0%
AAGGGTTCGACTGCCTCAAC+AGG + chr5_1:68375382-68375401 Msa0746220:CDS 55.0%
AGGGTTCGACTGCCTCAACA+GGG + chr5_1:68375383-68375402 Msa0746220:CDS 55.0%
CAGCAGGGACGCTTGAATTC+TGG - chr5_1:68375413-68375432 None:intergenic 55.0%
GTCCTAAGCCACGACGAGTC+GGG + chr5_1:68374006-68374025 Msa0746220:CDS 60.0%
TCCTAAGCCACGACGAGTCG+GGG + chr5_1:68374007-68374026 Msa0746220:CDS 60.0%
CCCCGTGACTCTCCTGTTGA+AGG - chr5_1:68375152-68375171 None:intergenic 60.0%
TTTCCGTGTCTCCACCGGTC+AGG + chr5_1:68375211-68375230 Msa0746220:CDS 60.0%
TTCCGTGTCTCCACCGGTCA+GGG + chr5_1:68375212-68375231 Msa0746220:CDS 60.0%
GGTCAGGGCTGCAAATCCCT+TGG + chr5_1:68375227-68375246 Msa0746220:CDS 60.0%
GGCTGCAAATCCCTTGGTCC+AGG + chr5_1:68375233-68375252 Msa0746220:CDS 60.0%
CCGGCTGCCGTTAGAGTAGA+AGG + chr5_1:68375363-68375382 Msa0746220:CDS 60.0%
! ACCCCGACTCGTCGTGGCTT+AGG - chr5_1:68374011-68374030 None:intergenic 65.0%
! GACAAGACCCCGACTCGTCG+TGG - chr5_1:68374017-68374036 None:intergenic 65.0%
AGCCCTGACCGGTGGAGACA+CGG - chr5_1:68375217-68375236 None:intergenic 65.0%
GGATTTGCAGCCCTGACCGG+TGG - chr5_1:68375225-68375244 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 68372969 68375868 68372969 ID=Msa0746220;Name=Msa0746220
chr5_1 mRNA 68372969 68375868 68372969 ID=Msa0746220-mRNA-1;Parent=Msa0746220;Name=Msa0746220-mRNA-1;_AED=0.01;_eAED=0.01;_QI=176|1|1|1|0.66|0.5|4|428|168
chr5_1 exon 68372969 68373109 68372969 ID=Msa0746220-mRNA-1:exon:20364;Parent=Msa0746220-mRNA-1
chr5_1 exon 68373877 68374072 68373877 ID=Msa0746220-mRNA-1:exon:20365;Parent=Msa0746220-mRNA-1
chr5_1 exon 68374167 68374230 68374167 ID=Msa0746220-mRNA-1:exon:20366;Parent=Msa0746220-mRNA-1
chr5_1 exon 68375159 68375868 68375159 ID=Msa0746220-mRNA-1:exon:20367;Parent=Msa0746220-mRNA-1
chr5_1 five_prime_UTR 68372969 68373109 68372969 ID=Msa0746220-mRNA-1:five_prime_utr;Parent=Msa0746220-mRNA-1
chr5_1 five_prime_UTR 68373877 68373911 68373877 ID=Msa0746220-mRNA-1:five_prime_utr;Parent=Msa0746220-mRNA-1
chr5_1 CDS 68373912 68374072 68373912 ID=Msa0746220-mRNA-1:cds;Parent=Msa0746220-mRNA-1
chr5_1 CDS 68374167 68374230 68374167 ID=Msa0746220-mRNA-1:cds;Parent=Msa0746220-mRNA-1
chr5_1 CDS 68375159 68375440 68375159 ID=Msa0746220-mRNA-1:cds;Parent=Msa0746220-mRNA-1
chr5_1 three_prime_UTR 68375441 68375868 68375441 ID=Msa0746220-mRNA-1:three_prime_utr;Parent=Msa0746220-mRNA-1
Gene Sequence

>Msa0746220

ATGAACCGTTACAGTCTTCAGCAGAACGCCTTTGCAGCTTGTGAAGAGATGAGAGGCTCAGTTACCATTACTGATCACAAGGAGCCTCTCATTTGTCCTAAGCCACGACGAGTCGGGGTCTTGTCGAACGTGCCTGTGCGACAATTGAGATTTCATTTCAATCAACAAGCTGAGGGTTCTGATTCAAAAGCTGCGGCTGAGCTGCTTGACATGATTTTTGAGAAGGAGAGTCACGGGGACGAGTTTGCTAATCATGTAGCCTCATCTCCTCCATATTTTTCCGTGTCTCCACCGGTCAGGGCTGCAAATCCCTTGGTCCAGGATGCTCGATTTGGAAACGAGATCATATCATCATCCCCATCAGGCTTGCCATCTCCAAATTCTGCATCCCGCAAAGGTGGATGTGTTAGGATGAGTTTTGGACTTAAACCGGCTGCCGTTAGAGTAGAAGGGTTCGACTGCCTCAACAGGGATCGCCAGAATTCAAGCGTCCCTGCTGTCGCTTAA
Protein sequence

>Msa0746220

MNRYSLQQNAFAACEEMRGSVTITDHKEPLICPKPRRVGVLSNVPVRQLRFHFNQQAEGSDSKAAAELLDMIFEKESHGDEFANHVASSPPYFSVSPPVRAANPLVQDARFGNEIISSSPSGLPSPNSASRKGGCVRMSFGLKPAAVRVEGFDCLNRDRQNSSVPAVA*