AlfalfaGEDB | Msa0746550

M. sativa cultivar zhongmu-4 / Msa0746550

KEGG: -
GO: GO:0005575 GO:0005911 GO:0006950 GO:0006952 GO:0008150 GO:0009506 GO:0009605 GO:0009607 GO:0009617 GO:0030054 GO:0042742 GO:0043207 GO:0050896 GO:0051704 GO:0051707 GO:0055044 GO:0098542
PFAM: PF03168.16

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0746550	XP_013461074.1	90.476	252	19	1	1	247	1	252	3.63e-146	419

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0746550	sp\|Q9ZVD2\|NHL13_ARATH	52.107	261	111	2	1	248	1	260	2.20e-96	285

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0746550	A0A072V156	90.476	252	19	1	1	247	1	252	1.74e-146	419

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0710810	Msa0746550	0.804044	2.609127e-49	-8.615850e-47
Msa0746550	Msa0810250	0.848689	5.223505e-60	-8.615850e-47
Msa0746550	Msa0853250	0.803515	3.360759e-49	-8.615850e-47
Msa0746550	Msa0881750	0.806787	6.942620e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0746550	MtrunA17_Chr3g0118371	90.476	252	19	1	1	247	1	252	3.34e-150	419
Msa0746550	MtrunA17_Chr5g0440571	63.672	256	84	3	1	248	1	255	1.59e-118	338
Msa0746550	MtrunA17_Chr2g0281401	49.615	260	109	5	1	241	1	257	1.85e-79	240
Msa0746550	MtrunA17_Chr3g0118381	67.808	146	45	2	104	247	1	146	4.78e-68	206
Msa0746550	MtrunA17_Chr3g0118391	56.784	199	84	2	43	241	4	200	5.00e-65	201
Msa0746550	MtrunA17_Chr1g0188801	32.669	251	158	5	3	249	7	250	4.10e-34	123
Msa0746550	MtrunA17_Chr5g0433201	32.197	264	161	6	1	247	1	263	1.33e-33	122
Msa0746550	MtrunA17_Chr3g0109121	36.782	174	99	3	81	249	85	252	1.08e-29	111
Msa0746550	MtrunA17_Chr3g0115651	27.876	226	142	7	13	230	16	228	2.50e-26	103
Msa0746550	MtrunA17_Chr8g0382431	28.448	232	144	6	24	248	43	259	4.72e-23	94.4

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0746550	AT2G27080.1	52.107	261	111	2	1	248	1	260	2.24e-97	285
Msa0746550	AT2G27080.2	52.107	261	111	2	1	248	1	260	2.24e-97	285
Msa0746550	AT5G21130.1	45.415	229	124	1	19	247	53	280	2.32e-70	218
Msa0746550	AT5G36970.1	33.591	259	149	9	1	247	1	248	7.51e-33	120
Msa0746550	AT1G65690.1	32.653	245	158	4	7	249	13	252	5.35e-29	110
Msa0746550	AT1G54540.1	32.143	252	153	7	1	247	1	239	5.76e-28	107
Msa0746550	AT2G35980.1	32.984	191	120	6	36	224	16	200	1.15e-23	95.5
Msa0746550	AT2G22180.1	27.966	236	140	8	19	245	75	289	2.50e-20	88.2
Msa0746550	AT3G11650.1	27.723	202	128	5	36	224	17	213	7.42e-17	77.8
Msa0746550	AT5G22870.1	25.946	185	125	4	62	241	26	203	1.54e-16	76.3

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Find 65 sgRNAs with CRISPR-Local

Find 142 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATAGTAGATAAAGATGTT+AGG	0.480050	5_1:-68974590	None:intergenic
AACACCGTCACATTATTCGA+CGG	0.648636	5_1:-68974750	None:intergenic
AACGTTGAAGCTGAGAGCAC+CGG	0.589685	5_1:+68974863	Msa0746550:CDS
ACACGATCGATCGTGTATTT+CGG	0.396195	5_1:-68974549	None:intergenic
ACACTCGCCGCAAAAGTCGC+CGG	0.610216	5_1:+68974418	Msa0746550:CDS
ACCGCTCCATCGCCGCCGTC+CGG	0.448630	5_1:+68974318	Msa0746550:CDS
AGAGGCGTTAGTCTCTGTAA+CGG	0.674629	5_1:+68974705	Msa0746550:CDS
AGCGGTGTTGGAAGGTGGAG+AGG	0.599059	5_1:-68974301	None:intergenic
AGCGTTGGTGAATGCGGTTA+CGG	0.509259	5_1:+68974824	Msa0746550:CDS
AGGATCTGTGAAGACGTGGA+AGG	0.675912	5_1:+68974902	Msa0746550:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTAACAGAAACATCAAACTC+GGG	-	chr5_1:68974618-68974637	None:intergenic	30.0%
GTTTCTGTTAAAGCTGATAA+CGG	+	chr5_1:68974627-68974646	Msa0746550:CDS	30.0%
TTAACAGAAACATCAAACTC+GGG	-	chr5_1:68974618-68974637	None:intergenic	30.0%
GTTTCTGTTAAAGCTGATAA+CGG	+	chr5_1:68974627-68974646	Msa0746550:CDS	30.0%
TAAGGTTGACTGTGATCTAA+CGG	+	chr5_1:68974932-68974951	Msa0746550:CDS	35.0%
TAAGGTTGACTGTGATCTAA+CGG	+	chr5_1:68974932-68974951	Msa0746550:CDS	35.0%
ATTTACTCTGATCGTGCTCA+TGG	+	chr5_1:68974482-68974501	Msa0746550:CDS	40.0%
GTAAAAAATACCAGCAGCGA+TGG	-	chr5_1:68974508-68974527	None:intergenic	40.0%
ACACGATCGATCGTGTATTT+CGG	-	chr5_1:68974552-68974571	None:intergenic	40.0%
TGTTAGGTTCATTCCCTTGA+CGG	-	chr5_1:68974577-68974596	None:intergenic	40.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_1	gene	68974276	68975025	68974276	ID=Msa0746550;Name=Msa0746550
chr5_1	mRNA	68974276	68975025	68974276	ID=Msa0746550-mRNA-1;Parent=Msa0746550;Name=Msa0746550-mRNA-1;_AED=0.38;_eAED=0.39;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|249
chr5_1	exon	68974276	68975025	68974276	ID=Msa0746550-mRNA-1:exon:20550;Parent=Msa0746550-mRNA-1
chr5_1	CDS	68974276	68975025	68974276	ID=Msa0746550-mRNA-1:cds;Parent=Msa0746550-mRNA-1

Gene Sequence

>Msa0746550

ATGGCTGATCGTGTTCACCCTAACGCCTCTCCACCTTCCAACACCGCTCCATCGCCGCCGTCCGGAACATATGTCATCCAGCTCCCCAAGGATCAAACCTACCGCATCCCACCACCAGAGAATGCACAACGTTACGCCAACTACACTCGCCGCAAAAGTCGCCGGTGCGGATGCTGTTGCTGTCTTTGCTGGTTCATCGGTATTTTATTTACTCTGATCGTGCTCATGGCCATCGCTGCTGGTATTTTTTACTTGATTGTTCGGCCGGAAGCACCGAAATACACGATCGATCGTGTCTCCGTCAAGGGAATGAACCTAACATCTTTATCTACTATTTCACCCGAGTTTGATGTTTCTGTTAAAGCTGATAACGGTAACAAAAAGATCGGAATCTACTACGAAAGCGATAGCAGCGTTGAAATGTTTTACAGAGGCGTTAGTCTCTGTAACGGCGTGTTGCCAGTGTTTTATCAGCCGTCGAATAATGTGACGGTGTTTCAGACGGTGTTGAAAGGTAACGGTATCGAACTTGCGAGATCGGATCAGAGAGCGTTGGTGAATGCGGTTACGGAACGAAGCGTGCCGTTAACGTTGAAGCTGAGAGCACCGGTGAAGATTAAAGTTGGAGGATCTGTGAAGACGTGGAAGGTTAGAGTTAAGGTTGACTGTGATCTAACGGTGGATCAGTTAACGGCGCAGGCGAAGATTGTTGACAAGGATTGTAGTTATGAATTTGATCTTTGGTTATGA

Protein sequence

>Msa0746550

MADRVHPNASPPSNTAPSPPSGTYVIQLPKDQTYRIPPPENAQRYANYTRRKSRRCGCCCCLCWFIGILFTLIVLMAIAAGIFYLIVRPEAPKYTIDRVSVKGMNLTSLSTISPEFDVSVKADNGNKKIGIYYESDSSVEMFYRGVSLCNGVLPVFYQPSNNVTVFQTVLKGNGIELARSDQRALVNAVTERSVPLTLKLRAPVKIKVGGSVKTWKVRVKVDCDLTVDQLTAQAKIVDKDCSYEFDLWL*