AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0748220

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0748220 MtrunA17_Chr7g0255911 98.969 97 1 0 1 97 1 97 1.53e-63 187
Msa0748220 MtrunA17_Chr3g0139681 74.227 97 25 0 1 97 1 97 3.84e-48 148
Msa0748220 MtrunA17_Chr3g0117191 64.706 85 30 0 1 85 1 85 4.72e-35 115
Msa0748220 MtrunA17_Chr3g0117431 74.359 39 10 0 1 39 1 39 4.18e-15 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0748220 AT1G14980.1 70.103 97 29 0 1 97 1 97 5.30e-47 145
Msa0748220 AT1G23100.1 69.072 97 30 0 1 97 1 97 5.62e-45 140
Msa0748220 AT1G14980.2 70.732 82 24 0 1 82 1 82 8.82e-38 122

Find 46 sgRNAs with CRISPR-Local

Find 170 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCCTCTGGTTTAGTTATTT+TGG 0.077085 5_2:+1374844 Msa0748220:three_prime_UTR
CAAAACATTTCTTAGAAATT+TGG 0.080809 5_2:+1374705 Msa0748220:three_prime_UTR
TCGCTTCGCCATTTCGAAAT+TGG 0.172281 5_2:-1371903 None:intergenic
GTCAAAGTCATTGGCGGATT+TGG 0.232289 5_2:+1374572 Msa0748220:three_prime_UTR
TTGTTGCTTTAGCTGAATTC+TGG 0.244257 5_2:+1373092 Msa0748220:intron
AAAACATTTCTTAGAAATTT+GGG 0.271475 5_2:+1374706 Msa0748220:three_prime_UTR
GTATGGAGATGACGATATAT+TGG 0.325927 5_2:+1374531 Msa0748220:CDS
TCCATTGTTCAACCGTGTTC+TGG 0.339609 5_2:+1371931 Msa0748220:CDS
GCTGTTGGCCCTGGCGTTCA+TGG 0.343925 5_2:+1373125 Msa0748220:CDS
TACATTCCATAGCCTGTTTG+TGG 0.344028 5_2:+1374801 Msa0748220:three_prime_UTR
TCACCTTCCTTTACAGCAAC+AGG 0.376842 5_2:-1373167 None:intergenic
AAAATACTAATCTTGATTGT+TGG 0.377725 5_2:-1374729 None:intergenic
ACAGGCTATGGAATGTAATA+AGG 0.379259 5_2:-1374795 None:intergenic
ACCTCAACTCCTCCGTATTC+AGG 0.397560 5_2:-1373203 None:intergenic
TTTGGCAGGTATTATCTGTA+TGG 0.419891 5_2:+1374514 Msa0748220:intron
ACTCCTTCCTGTTGCTGTAA+AGG 0.425958 5_2:+1373160 Msa0748220:CDS
TAAATTTAAATGCATGCCTC+TGG 0.426058 5_2:+1374830 Msa0748220:three_prime_UTR
CGTTTGCTCCAATTTCGAAA+TGG 0.427778 5_2:+1371895 Msa0748220:exon
AATTTAAATAAGCCACAAAC+AGG 0.443958 5_2:-1374813 None:intergenic
AGTATATAAATTACTACAGC+AGG 0.450662 5_2:-1374597 None:intergenic
CCGTCCTTACCATGAACGCC+AGG 0.451462 5_2:-1373134 None:intergenic
TATGGAGATGACGATATATT+GGG 0.451979 5_2:+1374532 Msa0748220:CDS
AATAAGCCACAAACAGGCTA+TGG 0.490821 5_2:-1374807 None:intergenic
TGGCCCTGGCGTTCATGGTA+AGG 0.497703 5_2:+1373130 Msa0748220:CDS
ACTGTTCTGTTACCTGAATA+CGG 0.515420 5_2:+1373191 Msa0748220:CDS
ATGATTGATGTCAAAGTCAT+TGG 0.522903 5_2:+1374563 Msa0748220:exon
AAAGTTGTTGCTGTTGGCCC+TGG 0.524580 5_2:+1373116 Msa0748220:CDS
GCTGTAGTAATTTATATACT+AGG 0.535706 5_2:+1374600 Msa0748220:three_prime_UTR
TCTGGAAAAGTTGTTGCTGT+TGG 0.541060 5_2:+1373110 Msa0748220:CDS
ATTGATGTCAAAGTCATTGG+CGG 0.544417 5_2:+1374566 Msa0748220:exon
CGGTAACAAAATTCCAGCGT+TGG 0.547494 5_2:-1371981 None:intergenic
ACCTGAATACGGAGGAGTTG+AGG 0.577975 5_2:+1373202 Msa0748220:CDS
CTTCCTGTTGCTGTAAAGGA+AGG 0.582243 5_2:+1373164 Msa0748220:CDS
ACCAGAACACGGTTGAACAA+TGG 0.591274 5_2:-1371932 None:intergenic
CCTGGCGTTCATGGTAAGGA+CGG 0.598384 5_2:+1373134 Msa0748220:CDS
GTTACCGGAGAAAATCTCCA+AGG 0.620509 5_2:+1371997 Msa0748220:CDS
GTATATAAATTACTACAGCA+GGG 0.622541 5_2:-1374596 None:intergenic
GTCCAAAATAACTAAACCAG+AGG 0.622937 5_2:-1374846 None:intergenic
CTTCCTTTACAGCAACAGGA+AGG 0.636048 5_2:-1373163 None:intergenic
CAATCTTCTCAACCAGAACA+CGG 0.641660 5_2:-1371943 None:intergenic
GAGCAAACGACAGCAACACA+AGG 0.644728 5_2:-1371881 None:intergenic
ATGGTATGTTTGAACATGCA+TGG 0.648776 5_2:-1374905 None:intergenic
TATATAAATTACTACAGCAG+GGG 0.649865 5_2:-1374595 None:intergenic
CGTCCTTACCATGAACGCCA+GGG 0.657122 5_2:-1373133 None:intergenic
GAATGTAATAAGGTACGTTG+CGG 0.660109 5_2:-1374785 None:intergenic
GTTCTGTTACCTGAATACGG+AGG 0.813129 5_2:+1373194 Msa0748220:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTAAAATCTTAAACATTT+TGG - chr5_2:1372793-1372812 None:intergenic 10.0%
!!! GTTTCTTAGTTTTAATTTAT+TGG + chr5_2:1373326-1373345 Msa0748220:intron 15.0%
!! ATAAAAATACATTATACCAA+TGG - chr5_2:1373704-1373723 None:intergenic 15.0%
!! TAAAAAAAATTGAATAGAGA+TGG - chr5_2:1373806-1373825 None:intergenic 15.0%
!!! ATATTTTCATAGTCTTAATT+AGG + chr5_2:1373825-1373844 Msa0748220:intron 15.0%
!! TTTGTAATTTACATTCTATA+TGG - chr5_2:1374002-1374021 None:intergenic 15.0%
!! TAGAATGTAAATTACAAAAT+TGG + chr5_2:1374004-1374023 Msa0748220:intron 15.0%
!!! TGAATTTTTTAATATTGTCA+TGG + chr5_2:1374040-1374059 Msa0748220:intron 15.0%
!! AAAAACTAGGAATTTATTTA+GGG - chr5_2:1374143-1374162 None:intergenic 15.0%
!! CAAAAAAATATGATCTAATT+AGG - chr5_2:1374498-1374517 None:intergenic 15.0%
!!! CTAATTAGATCATATTTTTT+TGG + chr5_2:1374496-1374515 Msa0748220:intron 15.0%
!! AAAACATTTCTTAGAAATTT+GGG + chr5_2:1374706-1374725 Msa0748220:three_prime_UTR 15.0%
!!! ATGCAAATAAATAAGCTTTT+AGG + chr5_2:1372297-1372316 Msa0748220:intron 20.0%
!! TCAAAAAACAAACATTTGTT+TGG - chr5_2:1372495-1372514 None:intergenic 20.0%
!! AACAATTATTCTATAAGAAC+CGG - chr5_2:1372531-1372550 None:intergenic 20.0%
!! TAGATAATCAAAATTCTACT+AGG - chr5_2:1372658-1372677 None:intergenic 20.0%
!! ATGTATATCATAAAAATGCA+TGG - chr5_2:1372895-1372914 None:intergenic 20.0%
!! ACCAAGAAAATAAATTATCT+AGG - chr5_2:1373540-1373559 None:intergenic 20.0%
!! TAATTTACATTCTATATGGT+TGG - chr5_2:1373998-1374017 None:intergenic 20.0%
!! TATGATATACATTGAATTGA+AGG + chr5_2:1374087-1374106 Msa0748220:intron 20.0%
!! AAAACTAGGAATTTATTTAG+GGG - chr5_2:1374142-1374161 None:intergenic 20.0%
!! GAAAAACTAGGAATTTATTT+AGG - chr5_2:1374144-1374163 None:intergenic 20.0%
!! TAATCATAATGATATGTAGT+CGG + chr5_2:1374391-1374410 Msa0748220:intron 20.0%
!! CAAAACATTTCTTAGAAATT+TGG + chr5_2:1374705-1374724 Msa0748220:three_prime_UTR 20.0%
!! AAAATACTAATCTTGATTGT+TGG - chr5_2:1374732-1374751 None:intergenic 20.0%
!!! TAGTATTTTTATGCAACTAT+TGG + chr5_2:1374743-1374762 Msa0748220:three_prime_UTR 20.0%
!!! AGTATTTTTATGCAACTATT+GGG + chr5_2:1374744-1374763 Msa0748220:three_prime_UTR 20.0%
!! TATGTTCTTATCACACTTTT+AGG + chr5_2:1372141-1372160 Msa0748220:intron 25.0%
! ATATTCATCTATTCTGTCTT+AGG + chr5_2:1372230-1372249 Msa0748220:intron 25.0%
! TATTCATCTATTCTGTCTTA+GGG + chr5_2:1372231-1372250 Msa0748220:intron 25.0%
! AAACAAACATTTGTTTGGTT+TGG - chr5_2:1372490-1372509 None:intergenic 25.0%
!! TTTTGACGTTGTTTATATTC+CGG + chr5_2:1372509-1372528 Msa0748220:intron 25.0%
! AATTTGAATGTTCTTGATTC+AGG + chr5_2:1372809-1372828 Msa0748220:intron 25.0%
! ATTTGAATGTTCTTGATTCA+GGG + chr5_2:1372810-1372829 Msa0748220:intron 25.0%
! TGGTTTATGATAGAACTTAA+TGG + chr5_2:1372970-1372989 Msa0748220:intron 25.0%
! TTAATTCAATTAGTCAGTTG+AGG + chr5_2:1373443-1373462 Msa0748220:intron 25.0%
! TAATTCAATTAGTCAGTTGA+GGG + chr5_2:1373444-1373463 Msa0748220:intron 25.0%
! AAATTATCTAGGCATCATAT+GGG - chr5_2:1373529-1373548 None:intergenic 25.0%
! TAAATTATCTAGGCATCATA+TGG - chr5_2:1373530-1373549 None:intergenic 25.0%
!!! GCCTAGATAATTTATTTTCT+TGG + chr5_2:1373536-1373555 Msa0748220:intron 25.0%
!! TTTAAATTGCTGTTGAATGT+TGG + chr5_2:1373580-1373599 Msa0748220:intron 25.0%
! AAAATACATTATACCAATGG+TGG - chr5_2:1373701-1373720 None:intergenic 25.0%
!! TTTTTAATATTGTCATGGTC+TGG + chr5_2:1374045-1374064 Msa0748220:intron 25.0%
!! TTTTAATATTGTCATGGTCT+GGG + chr5_2:1374046-1374065 Msa0748220:intron 25.0%
!! ATATCATACATTTTCAGAAC+TGG - chr5_2:1374075-1374094 None:intergenic 25.0%
! TATAAACTTTGTGAAAGAGA+AGG - chr5_2:1374176-1374195 None:intergenic 25.0%
! TGTGAATAAATTAGATGTGA+TGG + chr5_2:1374417-1374436 Msa0748220:intron 25.0%
!!! TTAGATCATATTTTTTTGGC+AGG + chr5_2:1374500-1374519 Msa0748220:intron 25.0%
! TATATAAATTACTACAGCAG+GGG - chr5_2:1374598-1374617 None:intergenic 25.0%
! GTATATAAATTACTACAGCA+GGG - chr5_2:1374599-1374618 None:intergenic 25.0%
! AGTATATAAATTACTACAGC+AGG - chr5_2:1374600-1374619 None:intergenic 25.0%
! GCTGTAGTAATTTATATACT+AGG + chr5_2:1374600-1374619 Msa0748220:three_prime_UTR 25.0%
!!! ACAATCTATATCTTGTTTTG+AGG + chr5_2:1374682-1374701 Msa0748220:three_prime_UTR 25.0%
!!! GTATTTTTATGCAACTATTG+GGG + chr5_2:1374745-1374764 Msa0748220:three_prime_UTR 25.0%
! AATTTAAATAAGCCACAAAC+AGG - chr5_2:1374816-1374835 None:intergenic 25.0%
TTGATTGTTGATCATGACAT+TGG + chr5_2:1372198-1372217 Msa0748220:intron 30.0%
!! TTTGTTTGGTTTGGTAACAA+GGG - chr5_2:1372481-1372500 None:intergenic 30.0%
!! ATTTGTTTGGTTTGGTAACA+AGG - chr5_2:1372482-1372501 None:intergenic 30.0%
!! GCTTCTAGGATTTCTAAAAT+AGG + chr5_2:1372717-1372736 Msa0748220:intron 30.0%
!! CAGGGTATTTTTCGATTTTA+TGG + chr5_2:1372828-1372847 Msa0748220:intron 30.0%
GTTCATTTCATTAGACTGAT+TGG + chr5_2:1372850-1372869 Msa0748220:intron 30.0%
TATGATATACATTCGTGCTT+TGG + chr5_2:1372903-1372922 Msa0748220:intron 30.0%
AAACTTTCATAATCAGCTTC+AGG - chr5_2:1373425-1373444 None:intergenic 30.0%
CAACAGCAATTTAAACATGA+AGG - chr5_2:1373575-1373594 None:intergenic 30.0%
! ACAAAGCTAGTCCTTATTTA+TGG + chr5_2:1373623-1373642 Msa0748220:intron 30.0%
TATCCTTTCCTCCATAAATA+AGG - chr5_2:1373637-1373656 None:intergenic 30.0%
AAACATTGACATGCAATATC+AGG - chr5_2:1373662-1373681 None:intergenic 30.0%
TGATATTGCATGTCAATGTT+TGG + chr5_2:1373661-1373680 Msa0748220:intron 30.0%
GATATTGCATGTCAATGTTT+GGG + chr5_2:1373662-1373681 Msa0748220:intron 30.0%
!! ATTTGATTTTACTCCACCAT+TGG + chr5_2:1373685-1373704 Msa0748220:intron 30.0%
TCAACAGTATATAAGTGAGA+AGG - chr5_2:1373917-1373936 None:intergenic 30.0%
TTCTCACTTATATACTGTTG+AGG + chr5_2:1373916-1373935 Msa0748220:intron 30.0%
TCTCACTTATATACTGTTGA+GGG + chr5_2:1373917-1373936 Msa0748220:intron 30.0%
TACAAAATGTTGTCCAATTG+CGG + chr5_2:1374292-1374311 Msa0748220:intron 30.0%
TATGGAGATGACGATATATT+GGG + chr5_2:1374532-1374551 Msa0748220:CDS 30.0%
ATGATTGATGTCAAAGTCAT+TGG + chr5_2:1374563-1374582 Msa0748220:exon 30.0%
TAAATTTAAATGCATGCCTC+TGG + chr5_2:1374830-1374849 Msa0748220:three_prime_UTR 30.0%
! AAAACCTTGGAGATTTTCTC+CGG - chr5_2:1372004-1372023 None:intergenic 35.0%
AGAGGAGAATGAAAAAACCT+TGG - chr5_2:1372017-1372036 None:intergenic 35.0%
!! ATGGGTATGAATGGATTAAG+AGG - chr5_2:1372035-1372054 None:intergenic 35.0%
AAGAAAAACGAAGACCCAAA+AGG - chr5_2:1372068-1372087 None:intergenic 35.0%
! CAATTGAATTTTACACCGCA+AGG + chr5_2:1372595-1372614 Msa0748220:intron 35.0%
!! CCTTAATTTGTTCTACCTTG+CGG - chr5_2:1372613-1372632 None:intergenic 35.0%
CGCAAGGTAGAACAAATTAA+GGG + chr5_2:1372611-1372630 Msa0748220:intron 35.0%
AAGGTAGAACAAATTAAGGG+TGG + chr5_2:1372614-1372633 Msa0748220:intron 35.0%
GAAAAGGTTTACTAGGAACT+AGG - chr5_2:1372687-1372706 None:intergenic 35.0%
AGCTCAAGAAAAGGTTTACT+AGG - chr5_2:1372694-1372713 None:intergenic 35.0%
AGAAGCAAAAGCTCAAGAAA+AGG - chr5_2:1372703-1372722 None:intergenic 35.0%
! TTTCTTGAGCTTTTGCTTCT+AGG + chr5_2:1372703-1372722 Msa0748220:intron 35.0%
GAAAGATCTAGAGATAGAGT+TGG + chr5_2:1372938-1372957 Msa0748220:intron 35.0%
GATAGAGTTGGATCGAAATA+TGG + chr5_2:1372950-1372969 Msa0748220:intron 35.0%
!! TTGTTGCTTTAGCTGAATTC+TGG + chr5_2:1373092-1373111 Msa0748220:intron 35.0%
ACTGTTCTGTTACCTGAATA+CGG + chr5_2:1373191-1373210 Msa0748220:CDS 35.0%
CTTAATCATGACTGATCTGA+TGG + chr5_2:1373298-1373317 Msa0748220:intron 35.0%
CTTCAGGACTATTACTCAAA+CGG - chr5_2:1373409-1373428 None:intergenic 35.0%
AGTGATTGCAAGCGTAATAT+AGG - chr5_2:1373470-1373489 None:intergenic 35.0%
! AAGCTAGTCCTTATTTATGG+AGG + chr5_2:1373626-1373645 Msa0748220:intron 35.0%
! AGTCCTTATTTATGGAGGAA+AGG + chr5_2:1373631-1373650 Msa0748220:intron 35.0%
AGGATTGCACAAGAAAAACT+AGG - chr5_2:1374156-1374175 None:intergenic 35.0%
TTGCAGAACGTAGAAAATAG+TGG - chr5_2:1374353-1374372 None:intergenic 35.0%
TTTGGCAGGTATTATCTGTA+TGG + chr5_2:1374514-1374533 Msa0748220:intron 35.0%
GTATGGAGATGACGATATAT+TGG + chr5_2:1374531-1374550 Msa0748220:CDS 35.0%
ATTGATGTCAAAGTCATTGG+CGG + chr5_2:1374566-1374585 Msa0748220:exon 35.0%
!!! TTGGGGAAACCTTTTTTTGA+AGG + chr5_2:1374762-1374781 Msa0748220:three_prime_UTR 35.0%
GAATGTAATAAGGTACGTTG+CGG - chr5_2:1374788-1374807 None:intergenic 35.0%
ACAGGCTATGGAATGTAATA+AGG - chr5_2:1374798-1374817 None:intergenic 35.0%
!! TGCCTCTGGTTTAGTTATTT+TGG + chr5_2:1374844-1374863 Msa0748220:three_prime_UTR 35.0%
GTCCAAAATAACTAAACCAG+AGG - chr5_2:1374849-1374868 None:intergenic 35.0%
CGTTTGCTCCAATTTCGAAA+TGG + chr5_2:1371895-1371914 Msa0748220:exon 40.0%
CAATCTTCTCAACCAGAACA+CGG - chr5_2:1371946-1371965 None:intergenic 40.0%
!!! ATTCCAGCGTTGGTTTTTGA+TGG - chr5_2:1371974-1371993 None:intergenic 40.0%
! CAACGCTGGAATTTTGTTAC+CGG + chr5_2:1371982-1372001 Msa0748220:CDS 40.0%
GGAGATGAAATGGGTATGAA+TGG - chr5_2:1372044-1372063 None:intergenic 40.0%
CCAAAAGGTGGAGATGAAAT+GGG - chr5_2:1372053-1372072 None:intergenic 40.0%
! CCATTTCATCTCCACCTTTT+GGG + chr5_2:1372051-1372070 Msa0748220:intron 40.0%
AAAAACGAAGACCCAAAAGG+TGG - chr5_2:1372065-1372084 None:intergenic 40.0%
! ATTCTGTCTTAGGGCTTGTT+TGG + chr5_2:1372240-1372259 Msa0748220:intron 40.0%
AGTAAAAGACCAATCCAGAC+AGG + chr5_2:1372455-1372474 Msa0748220:intron 40.0%
CCGCAAGGTAGAACAAATTA+AGG + chr5_2:1372610-1372629 Msa0748220:intron 40.0%
!! ATAGTCTTATCCCGCTAAGT+GGG - chr5_2:1373020-1373039 None:intergenic 40.0%
!! AATAGTCTTATCCCGCTAAG+TGG - chr5_2:1373021-1373040 None:intergenic 40.0%
TCTGGAAAAGTTGTTGCTGT+TGG + chr5_2:1373110-1373129 Msa0748220:CDS 40.0%
! GACCTTTTATGCGTCATTTG+CGG - chr5_2:1373377-1373396 None:intergenic 40.0%
ATTTATTTAGGGGCCAAGAG+AGG - chr5_2:1374132-1374151 None:intergenic 40.0%
AACAAACCGCTATTGTTCTG+CGG + chr5_2:1374260-1374279 Msa0748220:intron 40.0%
AACATACCGCAGAACAATAG+CGG - chr5_2:1374269-1374288 None:intergenic 40.0%
TACATTCCATAGCCTGTTTG+TGG + chr5_2:1374801-1374820 Msa0748220:three_prime_UTR 40.0%
AATAAGCCACAAACAGGCTA+TGG - chr5_2:1374810-1374829 None:intergenic 40.0%
TCGCTTCGCCATTTCGAAAT+TGG - chr5_2:1371906-1371925 None:intergenic 45.0%
TCCATTGTTCAACCGTGTTC+TGG + chr5_2:1371931-1371950 Msa0748220:CDS 45.0%
ACCAGAACACGGTTGAACAA+TGG - chr5_2:1371935-1371954 None:intergenic 45.0%
CGGTAACAAAATTCCAGCGT+TGG - chr5_2:1371984-1372003 None:intergenic 45.0%
GTTACCGGAGAAAATCTCCA+AGG + chr5_2:1371997-1372016 Msa0748220:CDS 45.0%
! CCCATTTCATCTCCACCTTT+TGG + chr5_2:1372050-1372069 Msa0748220:intron 45.0%
CCCAAAAGGTGGAGATGAAA+TGG - chr5_2:1372054-1372073 None:intergenic 45.0%
ACTCCTTCCTGTTGCTGTAA+AGG + chr5_2:1373160-1373179 Msa0748220:CDS 45.0%
CTTCCTTTACAGCAACAGGA+AGG - chr5_2:1373166-1373185 None:intergenic 45.0%
CTTCCTGTTGCTGTAAAGGA+AGG + chr5_2:1373164-1373183 Msa0748220:CDS 45.0%
TCACCTTCCTTTACAGCAAC+AGG - chr5_2:1373170-1373189 None:intergenic 45.0%
GTTCTGTTACCTGAATACGG+AGG + chr5_2:1373194-1373213 Msa0748220:CDS 45.0%
! CATGACTGATCTGATGGACA+CGG + chr5_2:1373304-1373323 Msa0748220:intron 45.0%
AGCCGCAAATGACGCATAAA+AGG + chr5_2:1373372-1373391 Msa0748220:intron 45.0%
AGACATGAAGCTCTGCATCA+AGG - chr5_2:1373760-1373779 None:intergenic 45.0%
! AGCTTCATGTCTGAAGCTGA+GGG + chr5_2:1373768-1373787 Msa0748220:intron 45.0%
GCTATAGCACTGTAGCATAG+CGG + chr5_2:1374231-1374250 Msa0748220:intron 45.0%
TCCAATTGCGGCTATAGCAT+TGG + chr5_2:1374304-1374323 Msa0748220:intron 45.0%
TCCAATGCTATAGCCGCAAT+TGG - chr5_2:1374308-1374327 None:intergenic 45.0%
! GCTATAGCATTGGAGCATAG+CGG + chr5_2:1374314-1374333 Msa0748220:intron 45.0%
!! GTCAAAGTCATTGGCGGATT+TGG + chr5_2:1374572-1374591 Msa0748220:three_prime_UTR 45.0%
ACGTTGCGGCCTTCAAAAAA+AGG - chr5_2:1374774-1374793 None:intergenic 45.0%
GAGCAAACGACAGCAACACA+AGG - chr5_2:1371884-1371903 None:intergenic 50.0%
!!! CCAGCGTTGGTTTTTGATGG+AGG - chr5_2:1371971-1371990 None:intergenic 50.0%
CCTCCATCAAAAACCAACGC+TGG + chr5_2:1371968-1371987 Msa0748220:CDS 50.0%
TAACAAGGGCCTGTCTGGAT+TGG - chr5_2:1372467-1372486 None:intergenic 50.0%
! TTTGGTAACAAGGGCCTGTC+TGG - chr5_2:1372472-1372491 None:intergenic 50.0%
ATGTAGCCGATCCCACTTAG+CGG + chr5_2:1373006-1373025 Msa0748220:intron 50.0%
CTTATCCCGCTAAGTGGGAT+CGG - chr5_2:1373015-1373034 None:intergenic 50.0%
! AAAGTTGTTGCTGTTGGCCC+TGG + chr5_2:1373116-1373135 Msa0748220:CDS 50.0%
ACCTGAATACGGAGGAGTTG+AGG + chr5_2:1373202-1373221 Msa0748220:CDS 50.0%
ACCTCAACTCCTCCGTATTC+AGG - chr5_2:1373206-1373225 None:intergenic 50.0%
! GAGCTTCATGTCTGAAGCTG+AGG + chr5_2:1373767-1373786 Msa0748220:intron 50.0%
CTGCAAGATGCATCCTCTCT+TGG + chr5_2:1374116-1374135 Msa0748220:intron 50.0%
GCTAAGTGGGATCGGCTACA+TGG - chr5_2:1373007-1373026 None:intergenic 55.0%
TGTAGCCGATCCCACTTAGC+GGG + chr5_2:1373007-1373026 Msa0748220:intron 55.0%
CGTCCTTACCATGAACGCCA+GGG - chr5_2:1373136-1373155 None:intergenic 55.0%
CCTGGCGTTCATGGTAAGGA+CGG + chr5_2:1373134-1373153 Msa0748220:CDS 55.0%
TGGCCCTGGCGTTCATGGTA+AGG + chr5_2:1373130-1373149 Msa0748220:CDS 60.0%
CCGTCCTTACCATGAACGCC+AGG - chr5_2:1373137-1373156 None:intergenic 60.0%
! ATGTCTGAAGCTGAGGGCGC+AGG + chr5_2:1373774-1373793 Msa0748220:intron 60.0%
GCTGTTGGCCCTGGCGTTCA+TGG + chr5_2:1373125-1373144 Msa0748220:CDS 65.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 1371863 1374920 1371863 ID=Msa0748220;Name=Msa0748220
chr5_2 mRNA 1371863 1374920 1371863 ID=Msa0748220-mRNA-1;Parent=Msa0748220;Name=Msa0748220-mRNA-1;_AED=0.01;_eAED=0.01;_QI=51|1|1|1|1|1|3|350|97
chr5_2 exon 1371863 1372018 1371863 ID=Msa0748220-mRNA-1:exon:320;Parent=Msa0748220-mRNA-1
chr5_2 exon 1373104 1373243 1373104 ID=Msa0748220-mRNA-1:exon:321;Parent=Msa0748220-mRNA-1
chr5_2 exon 1374522 1374920 1374522 ID=Msa0748220-mRNA-1:exon:322;Parent=Msa0748220-mRNA-1
chr5_2 five_prime_UTR 1371863 1371913 1371863 ID=Msa0748220-mRNA-1:five_prime_utr;Parent=Msa0748220-mRNA-1
chr5_2 CDS 1371914 1372018 1371914 ID=Msa0748220-mRNA-1:cds;Parent=Msa0748220-mRNA-1
chr5_2 CDS 1373104 1373243 1373104 ID=Msa0748220-mRNA-1:cds;Parent=Msa0748220-mRNA-1
chr5_2 CDS 1374522 1374570 1374522 ID=Msa0748220-mRNA-1:cds;Parent=Msa0748220-mRNA-1
chr5_2 three_prime_UTR 1374571 1374920 1374571 ID=Msa0748220-mRNA-1:three_prime_utr;Parent=Msa0748220-mRNA-1
Gene Sequence

>Msa0748220

ATGGCGAAGCGATTGATTCCATTGTTCAACCGTGTTCTGGTTGAGAAGATTGTTCCTCCATCAAAAACCAACGCTGGAATTTTGTTACCGGAGAAAATCTCCAAGCTGAATTCTGGAAAAGTTGTTGCTGTTGGCCCTGGCGTTCATGGTAAGGACGGCAAACTCCTTCCTGTTGCTGTAAAGGAAGGTGACACTGTTCTGTTACCTGAATACGGAGGAGTTGAGGTGAAGCTTGATCACAAAGAGTATTATCTGTATGGAGATGACGATATATTGGGAACACTGCATGATTGA
Protein sequence

>Msa0748220

MAKRLIPLFNRVLVEKIVPPSKTNAGILLPEKISKLNSGKVVAVGPGVHGKDGKLLPVAVKEGDTVLLPEYGGVEVKLDHKEYYLYGDDDILGTLHD*